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At least 559 records · Page 31

Continuous flow electrophoretic separation of proteins and cells from mammalian tissues

This paper describes an apparatus for continuous flow electrophoresis (CFE), designed to separate macromolecules and cells at conditions of microgravity. In this CFE, buffer flows upward in a 120-cm long flow chamber, which is 16-cm wide x 3.0-mm thick in the microgravity version (and 6-cm wide x 1.5-mm thick in the unit-gravity laboratory version). Ovalbumin and rat serum albumin were separated in space (flight STS-4) with the same resolution of the two proteins achieved at 25 percent total w/v concentration that was obtained in the laboratory at 0.2 percent w/v concentration. Rat anterior pituitary cells, cultured human embryonic kidney cells, and canine Langerhans cells were separated into subpopulations (flight STS-8) more effectively than in unit gravity, with comparable resolution having been achieved at 100 times the concentration possible on earth.

Hymer, W. C.↗

Control of the boundary layer separation about an airfoil by active surface heating

Application of active control to separated flow on the RC(6)-08 airfoil at high angle of attack by localized surface heating is numerically simulated by integrating the compressible two-dimensional nonlinear Navier-Stokes equations solver. Active control is simulated by local modification of the temperature boundary condition over a narrow strip on the upper surface of the airfoil. Both mean and perturbed profiles are favorably altered when excited with the same natural frequency of the shear layer by moderate surface heating for both laminar and turbulent separation. The shear layer is found to be very sensitive to localized surface heating in the vicinity of the separation point. The excitation field at the surface sufficiently altered both the local as well as the global circulation to cause a significant increase in lift and reduction in drag.

Maestrello, Lucio↗

Turbulent transport of He II in active and passive phase separators using slit devices and porous media

The turbulent transport mode of vapor liquid phase separators (VLPS) for He II has been investigated comparing passive porous plug separators with active phase separators (APS) using slits of variable flow paths within a common frame of reference. It is concluded that the basic transport regimes in both devices are identical. An integrated Gorter-Mellink (1949) equation, found previously to predict VLPS results of porous plugs, is employed to analyze APS data published in the literature. It is found that the Gorter-Mellink flow rate parameter for 9-micron and 14-micron APS slit widths are relatively independent of the slit width, having a rate constant of about 9 + or - 10 percent. This agrees with the early heat flow results for He II entropy transport at zero net mass flow in wide capillaries and slits.

Yuan, S. W. K.↗

Phase Separators And Fountain-Effect Pumps For He 11

Fused-glass microchannel arrays for use as HE 11 phase separators and fountain-effect pumps. Microchannel devices for use in low-gravity storage and cooling systems containing superfluid helium. Phase separators and pumps take advantage of thermomechanical effect peculiar to He 11 in restricted spaces. By creating thermal gradient in porous plug, direction of flow reversed, turning phase separator into pump. However, addition of heat disadvantage. Thermal gradient created by using Peltier effect to transfer heat across array, removing heat from He 11 supply and overcoming undesirable addition of heat.

Whitehouse, Paul L.↗

Study Of Phase Separation In Glass

Report describes an experimental study of effect of hydroxide content on phase separation in soda/silica glasses. Ordinary and gel glasses melted at 1,565 degree C, and melts stirred periodically. "Wet" glasses produced by passing bubbles of N2 saturated with water through melts; "dry" glasses prepared in similar manner, except N2 dried before passage through melts. Analyses of compositions of glasses performed by atomic-absorption and index-of-refraction measurements. Authors conclude hydroxide speeds up phase separation, regardless of method (gel or ordinary) by which glass prepared. Eventually helps material scientists to find ways to control morphology of phase separation.

Neilson, George F.↗

Demixing kinetics of phase separated polymer solutions in microgravity

Phase separated solutions of two neutral polymers in buffer provide a useful and versatile medium for the partition separation of biological cells. However, the efficiency of such separations is orders of magnitude lower than the thermodynamic limit. To test the hypothesis that this inefficiency is at least partially due to the convection and sedimentation that occur during the gravity driven demixing that follows introduction of cells to the systems, a series of experiments were begun aimed at performing cell partition in a low g environment. Demixing of isopycnic three polymer solvent systems was studied, experiments were performed on KC-135 aircraft and one shuttle middeck experiment was completed. Analysis of the results of these experiments and comparisons with the predictions of scaling relations for the dependence of phase domain size on time, derived for a number of possible demixing mechanisms, are presented.

Brooks, D. E.↗

On the Lagrangian description of unsteady boundary layer separation. Part 1: General theory

Although unsteady, high-Reynolds number, laminar boundary layers have conventionally been studied in terms of Eulerian coordinates, a Lagrangian approach may have significant analytical and computational advantages. In Lagrangian coordinates the classical boundary layer equations decouple into a momentum equation for the motion parallel to the boundary, and a hyperbolic continuity equation (essentially a conserved Jacobian) for the motion normal to the boundary. The momentum equations, plus the energy equation if the flow is compressible, can be solved independently of the continuity equation. Unsteady separation occurs when the continuity equation becomes singular as a result of touching characteristics, the condition for which can be expressed in terms of the solution of the momentum equations. The solutions to the momentum and energy equations remain regular. Asymptotic structures for a number of unsteady 3-D separating flows follow and depend on the symmetry properties of the flow. In the absence of any symmetry, the singularity structure just prior to separation is found to be quasi 2-D with a displacement thickness in the form of a crescent shaped ridge. Physically the singularities can be understood in terms of the behavior of a fluid element inside the boundary layer which contracts in a direction parallel to the boundary and expands normal to it, thus forcing the fluid above it to be ejected from the boundary layer.

Vandommelen, Leon L.↗

Oxygen separation from air using zirconia solid electrolyte membranes

Air separation using a zirconia solid electrolyte membrane is a possible alternative source of oxygen. The process of zirconia oxygen separation is reviewed, and an oxygen plant concept using such separation is described. Potential cell designs, stack designs, and testing procedures are examined. Fabrication of the materials used in a zirconia module as well as distribution plate design and fabrication are examined.

Suitor, J. W.↗

Turbulent flow separation control through passive techniques

Several passive separation control techniques for controlling moderate two-dimensional turbulent flow separation over a backward-facing ramp are studied. Small transverse and swept grooves, passive porous surfaces, large longitudinal grooves, and vortex generators were among the techniques used. It was found that, unlike the transverse and longitudinal grooves of an equivalent size, the 45-deg swept-groove configurations tested tended to enhance separation.

Lin, J. C.↗

Separation control on an airfoil by periodic forcing

The introduction of transverse velocity fluctuations into a separated shear layer on an airfoil at high angles of attack is presently demonstrated to be an effective separation-control technique. Airfoil aerodynamic characteristics, including poststall lift and drag as well as maximum lift coefficient and stall angle, all exhibited improvements controlled forcing at 20 deg angle of attack led to an increased spreading of the mean velocity profile, together with increased turbulence activity; separation moved from the leading edge to about 80 percent of chord.

Bar-Sever, A.↗

Acoustophoresis - A New Separation Concept

Ultrasound separates chemical species. Concept under development expands technology of chemical separation to include ultrasonic-radiation pressure. New technique separates species of particles according to ultrasonic properties. Acoustophoresis concept utilizes not only bulk compressional waves but also surface waves or boundary waves between solid (or liquid) container wall and subject liquid.

Heyman, Joseph S.↗

New test techniques to evaluate near field effects for supersonic store carriage and separation

Store separation and store carriage drag studies were conducted. A primary purpose is to develop new experimental methods to evaluate near field effects of store separation and levels of store carriage drag associated with a variety of carriage techniques for different store shapes and arrangements. Flow field measurements consisting of surface pressure distributions and vapor screen photographs are used to analyze the variations of the store separation characteristics with cavity geometry. Store carriage drag measurements representative of tangent, semi-submerged, and internal carriage installations are presented and discussed. Results are included from both fully metric models and models with only metric segments (metric pallets) and the relative merits of the two are discussed. Carriage drag measurements for store installations on an aircraft parent body are compared both with prediction methods and with installations on a generic parent body.

Sawyer, Wallace C.↗

Method and apparatus for detecting laminar flow separation and reattachment

The invention is a method and apparatus for detecting laminar flow separation and flow reattachment of a fluid stream by simultaneously sensing and comparing a plurality of output signals, each representing the dynamic shear stress at one of an equal number of sensors spaced along a straight line on the surface of an airfoil or the like that extends parallel to the fluid stream. The output signals are concurrently compared to detect the sensors across which a reversal in phase of said output signal occurs, said detected sensors being in the region of laminar separation or reattachment. The novelty in this invention is the discovery and use of the phase reversal phenomena to detect laminar separation and attachment of a fluid stream from any surface such as an airfoil supported therein.

Stack, John P.↗

Analysis of high-incidence separated flow past airfoils

An unsteady Navier-Stokes (NS) analysis is developed and used to carefully examine high-incidence aerodynamic separated flows past airfoils. Clustered conformal C-grids are employed for the 12 percent thick symmetric Joukowski airfoil as well as for the NACA 0012 airfoil with a sharp trailing edge. The clustering is controlled by appropriate one-dimensional stretching transformations. An attempt is made to resolve many of the dominant scales of an unsteady flow with massive separation, while maintaining the transformation metrics to be smooth and continuous in the entire flow field. A fully implicit time-marching alternating-direction implicit-block Gaussian elimination (ADI-BGE) method is employed, in which no use is made of any explicit artificial dissipation. Detailed results are obtained for massively separated, unsteady flow past symmetric Joukowski and NACA 0012 airfoils.

Chia, K. N.↗

On the Lagrangian description of unsteady boundary-layer separation. I - General theory

Although unsteady, high-Reynolds number, laminar boundary layers have conventionally been studied in terms of Eulerian coordinates, a Lagrangian approach may have significant analytical and computational advantages. In Lagrangian coordinates the classical boundary layer equations decouple into a momentum equation for the motion parallel to the boundary, and a hyperbolic continuity equation (essentially a conserved Jacobian) for the motion normal to the boundary. The momentum equations, plus the energy equation if the flow is compressible, can be solved independently of the continuity equation. Unsteady separation occurs when the continuity equation becomes singular as a result of touching characteristics, the condition for which can be expressed in terms of the solution of the momentum equations. The solutions to the momentum and energy equations remain regular. Asymptotic structures for a number of unsteady 3-D separating flows follow and depend on the symmetry properties of the flow. In the absence of any symmetry, the singularity structure just prior to separation is found to be quasi 2-D with a displacement thickness in the form of a crescent shaped ridge. Physically the singularities can be understood in terms of the behavior of a fluid element inside the boundary layer which contracts in a direction parallel to the boundary and expands normal to it, thus forcing the fluid above it to be ejected from the boundary layer.

Van Dommelen, Leon L.↗

Turbulent flow separation control over a backward-facing ramp via transverse and swept grooves

Separation control experiments were conducted in a 51-71-cm shear-flow control tunnel using a backward-facing ramp to investigate the performance of transverse and swept grooves for controlling a two-dimensional turbulent separated flow at low speeds and moderate Reynolds numbers. In the experiments, transverse grooves, located in the maximum +dP/dx region, with a height-to-width ratio greater than 2.5, reduced the reattachment distance by 20 percent over the baseline configuration. Unlike transverse and longitudinal grooves of equivalent size, the 45-degree swept-groove configurations tested enhanced separation.

Selby, G. V.↗

A computational study of incipient leading-edge separation on a 65-deg delta wing at M = 1.60

A computational study on a 65-deg delta wing at a freestream Mach number of 1.60 has been conducted by obtaining conical Reynolds-averaged Navier-Stokes solutions on a parametric series of geometries which varied in leading-edge radius and/or circular-arc camber. The computational results showed that increasing leading-edge radius or camber can delay the onset of leading-edge separation on the leeside of a delta wing at a specific angle of attack. Reynolds number was varied from 1 x 10 to the 6th to 5 x 10 to the 6th for a turbulent boundary-layer and was shown to have a minor effect on the effectiveness of leading-edge radius and/or camber in delaying the onset of leading-edge separation. Both laminar and turbulent boundary-layer models were investigated at a Reynolds number of 1 x 10 to the 6th, and the predicted flow pattern was found to change from attached flow for the turbulent boundary-layer model to separated flow for the laminar boundary-layer model. Based upon these results, three wind-tunnel models have been designed to be tested in the Langley Unitary Plan Wind Tunnel.

Mcmillin, S. Naomi↗

Application of localized surface heating to actively control the boundary layer separation

The use of localized surface heating to improve the performance of the airfoil of a fixed wing or rotating blade at high angle of attack is considered. Separation control by active means can be accomplished by acoustic perturbation of the separated area above the airfoil or by localized surface heating in a pressure gradient region. Active control by sound in the vicinity of the leading edge of an airfoil is shown to greatly reduce the area of separation. Therefore, the overall circulation is sufficiently changed to result in an increase in lift and a reduction in drag.

El-Hady, Nabit M.↗