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All sources NASA NTRS DOE OSTI

DOE OSTI2017

Benchmarking viromics: an in silico evaluation of metagenome-enabled estimates of viral community composition and diversity

Background: Viral metagenomics (viromics) is increasingly used to obtain uncultivated viral genomes, evaluate community diversity, and assess ecological hypotheses. While viromic experimental methods are relatively mature and widely accepted by the research community, robust bioinformatics standards remain to be established. Here we used in silico mock viral communities to evaluate the viromic sequence-to-ecological-inference pipeline, including (i) read pre-processing and metagenome assembly, (ii) thresholds applied to estimate viral relative abundances based on read mapping to assembled contigs, and (iii) normalization methods applied to the matrix of viral relative abundances for alpha and beta diversity estimates. Results: Tools specifically designed for metagenomes, specifically metaSPAdes, MEGAHIT, and IDBA-UD, were the most effective at assembling viromes. Read pre-processing, such as partitioning, had virtually no impact on assembly output, but may be useful when hardware is limited. Viral populations with 2–5 × coverage typically assembled well, whereas lesser coverage led to fragmented assembly. Strain heterogeneity within populations hampered assembly, especially when strains were closely related (average nucleotide identity, or ANI ≥97%) and when the most abundant strain represented <50% of the population. Viral community composition assessments based on read recruitment were generally accurate when the following thresholds for detection were applied: (i) ≥10 kb contig lengths to define populations, (ii) coverage defined from reads mapping at ≥90% identity, and (iii) ≥75% of contig length with ≥1 × coverage. Finally, although data are limited to the most abundant viruses in a community, alpha and beta diversity patterns were robustly estimated (±10%) when comparing samples of similar sequencing depth, but more divergent (up to 80%) when sequencing depth was uneven across the dataset. In the latter cases, the use of normalization methods specifically developed for metagenomes provided the best estimates. Conclusions: These simulations provide benchmarks for selecting analysis cut-offs and establish that an optimized sample-to-ecological-inference viromics pipeline is robust for making ecological inferences from natural viral communities. Continued development to better accessing RNA, rare, and/or diverse viral populations and improved reference viral genome availability will alleviate many of viromics remaining limitations.

59 BASIC BIOLOGICAL SCIENCES↗

NASA NTRS1975

Evaluation of methods of reducing community noise impact around San Jose municipal airport

A computer simulation of the airport noise impact on the surrounding communities was used to evaluate alternate operational procedures, improved technology, and land use conversion as methods of reducing community noise impact in the airport vicinity. In addition, a constant density population distribution was analyzed for possible application to other airport communities with fairly uniform population densities and similar aircraft operational patterns. The introduction of sound absorption material (SAM) was found to reduce community noise annoyance by over 25 percent, and the introduction of refan was found to reduce community annoyance by over 60 percent. Replacing the present aircraft was found to reduce the noise problem to very small proportions, and the introduction of an advanced technology twin was found to essentially eliminate the community noise problem.

Glick, J. M.↗

NASA NTRS2000

A Cybernetic Approach to the Modeling of Agent Communities

In an earlier paper [1] examples of agent technology in a NASA context were presented. Both groundbased and space-based applications were addressed. This paper continues the discussion of one aspect of the Goddard Space Flight Center's continuing efforts to develop a community of agents that can support both ground-based and space-based systems autonomy. The paper focuses on an approach to agent-community modeling based on the theory of viable systems developed by Stafford Beer. It gives the status of an initial attempt to capture some of the agent-community behaviors in a viable system context. This paper is expository in nature and focuses on a discussion of the modeling of some of the underlying concepts and infrastructure that will serve as the basis of more detailed investigative work into the behavior of agent communities. The paper is organized as follows. First, a general introduction to agent community requirements is presented. Secondly, a brief introduction to the cybernetic concept of a viable system is given. This concept forms the foundation of the modeling approach. Then the concept of an agent community is modeled in the cybernetic context.

Truszkowski, Walt↗

NASA NTRS2012

Microbial Lipid and C Isotopic Biosignatures of a Unique Community at Grand Prismatic Spring, Yellowstone National Park

The microbial communities found in modern hot springs are considered analogs to ones that may have existed in hydrothermal systems on the early Earth and possibly Mars. Our goal was to characterize the microbial biosignatures and to assess the preservation of organic matter in the silica-depositing Grand Prismatic Spring in Yellowstone National Park. This study combines 16S rRNA surveys, lipid biomarkers, and C isotopes to query, "Who's there and what are they doing?" On the edge of the approximately 90 m diameter blue vent pool (56.1 C, pH 8.5), a floating green streamer community grew over a benthic pink community. The membrane lipids in the green streamers and pink mat were composed of unusual ester-linked fatty acids, indicating the presence of novel bacterial groups. In particular, we discovered a series of 2-methyl and 2,X-dimethyl phospholipid fatty acids (C18-22). We are attempting to use the 16S rRNA surveys to link these compounds to source organisms. Wax esters, biomarkers for Chloroflexi, were present in both communities, but displayed different profiles. A higher proportion of branched wax esters were found in the green streamers, and were associated with a relatively high concentration of long-chain di- and trienes (C29-31). This suggests that Chloroflexus primarily grew in the green streamers, while a pink mat of Roseiflexus grew on the sinter substrate underneath. Cyanobacterial alkanes were found in the green streamers (n-C17, 7-, 6- and 5-monomethyl-C17, 7,11-dimethyl-C17, n-C19, n-C19:1). We also detected a series of monoalkylglycerylethers and geologically relevant hopanoids in both communities. Carbon isotope analyses indicated that Chloroflexus was growing photoheterotrophically using cyanobacterial photosynthate. Roseiflexus also traditionally grows photoheterotrophically, but the C isotopic signatures of the lipids in the pink mat were approximately 10 %0 lighter than the cyanobacterial and Chloroflexus lipids, indicating a potentially novel metabolic mechanism or prior secondary reworking of substrates before reaching Roseiflexus. This arrangement of Synechococcus-Chloroflexus green streamers floating over a benthic pink community of Roseiflexus is different from the classical laminated Synechococcus-Chloroflexi mats at Octopus Hot Spring.

Jahnke, Linda↗

NASA NTRSDate not supplied

Advancements in Lunar Dust Mitigation and Leveraging the Contamination Control Community

Lunar Dust Contamination: As we get closer to putting landers, rovers, payloads, and astronauts on the Moon, dust mitigation is becoming more critical than ever. To address the many challenges of dust mitigation on the surface, engineers and scientists are looking at various technologies that may have relevance for the contamination control community. This includes active, passive, architectural, and operational solutions. There have been advances in coatings for dust mitigation at NASA and through commercial partnerships (see Wiesner, Wohl, et al). There have been advancements to previous solutions as well as novel solutions to address dust mitigation on the surface and on-orbit. There have been several efforts to better understand the effectiveness of various terrestrial and dust mitigation solutions with much of this data published in recent years. In the area of dust mitigation, dust contamination or infiltration refers to the impingement or contact of planetary dust with items not normally dusty and whose operation may therefore be compromised. Recent Advancements in Dust Mitigation: Dust mitigation strategies are becoming more common for hardware heading to the Moon, with most major programs implementing lunar dust contamination requirements for human health and equipment. In the last couple of years, NASA has created more guidance to assist hardware developers with dust mitigation. This includes NASA-STD-1008 (Classifications and Requirements for Testing Systems and Hardware to be Exposed to Dust in Planetary) and NASA TP 20220018746 (Lunar Dust Mitigation Guide and Reference). Several projects are testing hardware in relevant dusty environments to understand their tolerance to lunar dust. There has been a significant increase in the amount of testing with lunar simulants, with the NASA Simulant Advisory Committee serving a resource for NASA and our partners. There are new solutions for detecting and monitoring the dust internally and externally. Learning from the contamination control community: In addition to sharing potentially relevant solutions at the workshop, the dust mitigation community is also interested in learning from the contamination control community on ways to address lunar dust. For instance, a major aspect of managing the dust is monitoring and detection. This is critical for understanding when maintenance is needed and to inform future missions. Another challenge of working with lunar dust is quantifying and characterizing the smallest of particles. There are also challenges in modeling lunar dust and its behavior. The dust mitigation community can also leverage lessons learned from the planetary protection community.

dust mitagation↗

NASA NTRS2024

A Survey of the Severity of Mental Health Symptoms in the Planetary Science Community

There is a growing recognition of a mental health crisis within the academic and research communities. Members of the planetary science community have called for healthier work environments to improve mental well-being. As a preliminary step towards improving workplace culture, we sought to determine if the broader mental health crisis extends to planetary science and assess the severity of anxiety, depressive, and stress symptoms. Our 2022 mental health survey of the planetary science community suggests that the severity of anxiety and depressive symptoms in the community is greater than in the general US population. Further, the anxiety and depressive symptoms are more severe for graduate students and postdoctoral researchers than any other career stage. Comparing groups within planetary science, we found that anxiety, depressive, and/or stress symptoms appear greater among marginalized groups, such as women, people of color, and members of the LGBTQ+ community. A mental health problem is impacting the planetary science community. Improving well-being will promote enhanced research quality and productivity.

David Trang↗

DOE OSTI2016

Port Hope Area Initiative - Community Based Concepts Become Reality - 16538

The Port Hope Area Initiative (PHAI) involves the cleanup of historic low-level radioactive waste (LLRW) resulting from radium and uranium processing by the former Crown Corporation, Eldorado Nuclear Limited, and its predecessor companies, during the period between 1932 and 1988. The PHAI, which is based on a legal agreement between the Government of Canada and the adjacent municipalities of Port Hope and Clarington in Ontario, Canada, comprises two separate community-initiated projects for the long-term management of the historic LLRW located in each of these municipalities. The Port Hope Project involves the cleanup of approximately 1.2 million cubic metres of historic LLRW from various sites within the urban area of Port Hope, the construction and operation of an engineered aboveground mound where the waste will be safely contained, and the long-term maintenance and monitoring of the new waste management facility. The waste is currently located in 13 large-scale sites and an estimated 375 small-scale sites within the community. In contrast, the Port Granby Project involves the relocation of approximately 450,000 cubic metres of historic LLRW from the existing waste site on the north shore of Lake Ontario in rural Clarington, to a new engineered aboveground mound to be built 700 metres north of the shoreline. The work also involves the restoration of the existing site and long-term maintenance and monitoring the new facility. The PHAI entered Phase 2 (construction) of a three-phase program in early 2012, when $1.28 billion (Canadian) in federal funding was announced by the Minister of Natural Resources Canada. The funding, together with regulatory approval from the Canadian Nuclear Safety Commission (CNSC), which granted 10-year waste management licences to both projects, have permitted the PHAI to move forward with construction and remediation. Waste water treatment plants based on best-available technologies have been constructed for each project to remove a wide range of contaminants from groundwater, surface water and leachate collected during construction and operation of the aboveground mounds. Each treatment process has been designed to ensure the quality of treated water being discharged into Lake Ontario meets or exceeds stringent regulatory requirements, resulting in the enhanced protection of the Great Lakes Basin ecosystem. In 2015, the contract to build the Port Granby long-term waste management facility and remediate the existing waste site was awarded, and the long-awaited facility development will soon begin. The use of a dedicated internal waste transportation route along, which the LLRW will be trucked from the existing site to the new facility, is a key component of the design to minimize the impact on the local, rural community. In Port Hope, the removal of contaminated soil within the site for the new long-term management facility commenced in the fall of 2015. Over the next several years, multiple contracts will be awarded for construction and operation of the new waste management facility and remediation of the large and small-scale sites. In short, the Port Hope and Port Granby community-based concepts have matured into tangible projects with appropriate regulatory approvals and federal funding in place. Ongoing communications with the two communities have resulted in a well-informed public that understands the work to be done and is eager to see their projects take shape and become reality. As Canada's largest environmental remediation and restoration undertaking, the PHAI has never been closer to fulfilling its vision - working with the local communities to fulfil Canada's commitment for the cleanup of historic waste in Port Hope and Port Granby to leave an honourable legacy for future generations. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

DOE OSTI2016

The Economic and Societal Impact of Baseload Power Generation on Local Communities

In the United States (US), energy from coal, natural gas, and nuclear power plants produced approximately 3,500 TWh of electricity and accounted for 85% of total net generation in 2015. In 2015, coal and natural gas power plants each contributed about 33% of all generation in the US. generation II nuclear power plants represents about 20% of all electricity produced in the US and 62.4% of emission-free electricity in 2015. As a byproduct of electricity, combustion fossil fuel plants release hazardous emissions such as CO{sub 2}, N{sub 2}O, NOx and SO{sub 2}. CO{sub 2}, CH{sub 4}, and N{sub 2}O. Many of these emissions are major greenhouse gases (GHG) that have been linked to contributing to global climate change. The release of NOx and SO{sub 2} have a negative impact on human health and contribute to smog and acid rain. As a result, communities are often subjected to negative externalities such as pollution, noise, undesirable visuals, and safety concerns when sited near power plants. Though nuclear power plants do not produce any air emissions, there are concerns of nuclear waste, radiation leaks into the environment, and Fukushima-level accidents. With electricity generation, there are economic, safety, and environmental tradeoffs between the three technologies. These factors can contribute to negative and positive externalities for communities that are sited near baseload power plants. Davis (2010) using the hedonic price method found that communities living within 2 miles of power plants experience a 3-7% decrease in housing values. There is further evidence that those that live even closer experience larger decreases. While several studies focus on hedonic price methods and health effects, none have investigated and compared the socioeconomic indicators of communities near baseload power plants. The goal of this work is to investigate and examine relationship between societal and economic community indicators with respect to distance and generation technology. From a cost benefit standpoint an argument can be made based on the externalities from these power plants. Positive externalities that improve median incomes and home values could mean added tax revenue for local municipalities and governments. Typically, comparisons between generating technologies are made using the levelized cost of electricity (LCOE) and life cycle GHG emissions, however the impacts on local communities can also be used as a metric when comparing technologies. (authors)

29 ENERGY PLANNING, POLICY, AND ECONOMY↗

DOE OSTI2013

The Ecology of Microbial Communities Associated with Macrocystis pyrifera

Kelp forests are characterized by high biodiversity and productivity, and the cycling of kelp-produced carbon is a vital process in this ecosystem. Although bacteria are assumed to play a major role in kelp forest carbon cycling, knowledge of the composition and diversity of these bacterial communities is lacking. Bacterial communities on the surface of Macrocystis pyrifera and adjacent seawater were sampled at the Hopkins Marine Station in Monterey Bay, CA, and further studied using 454-tag pyrosequencing of 16S RNA genes. Our results suggest that M. pyrifera-dominated kelp forests harbor distinct microbial communities that vary temporally. The distribution of sequence tags assigned to Gammaproteobacteria, Alphaproteobacteria and Bacteriodetes differed between the surface of the kelp and the surrounding water. Several abundant Rhodobacteraceae, uncultivated Gammaproteobacteria and Bacteriodetes-associated tags displayed considerable temporal variation, often with similar trends in the seawater and the surface of the kelp. Bacterial community structure and membership correlated with the kelp surface serving as host, and varied over time. Several kelp-specific taxa were highly similar to other bacteria known to either prevent the colonization of eukaryotic larvae or exhibit antibacterial activities. Some of these kelp-specific bacterial associations might play an important role for M. pyrifera. This study provides the first assessment of the diversity and phylogenetic profile of the bacterial communities associated with M. pyrifera.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2023

Community Resilience Options: A Menu for Enhancing Local Energy Resilience

This document highlights areas of potential community resilience improvements, especially those that relate to clean energy deployment for communities and municipalities. The National Renewable Energy Laboratory (NREL) defines resilience as "a system's ability to anticipate, prepare for, and adapt to changing conditions and withstand, respond to, and recover rapidly from disruptions through sustainable, adaptable, and holistic planning and technical solutions." This document introduces 10 categories of resilience-enhancing projects at a high level, intended for community members and decision-makers new to the topic to build their understanding of which solutions fit their community best. These categories focus primarily on community-scale measures and different options may be available at larger scales. Full implementation of the measures described here requires in-depth, site-specific considerations that go beyond the scope of this document.

29 ENERGY PLANNING, POLICY, AND ECONOMY↗

DOE OSTI2022

Community Planning for Solar: Defining Realistic Solar Development Options

This guide is designed to aid in assessing community preferences for solar development and financing alternatives. To evaluate community preferences regarding solar, it is necessary to define the range of realistic options for solar development possible in a community, including potential future targets for solar capacity, the mixes of development possible based on the solar resources available, and potential community benefits and solar ownership structures. This guide can help community officials, volunteers, and regional planning agency staff in constructing a realistic suite of options for solar development, based on existing state and local laws, available solar resources and infrastructure, and economic and financial considerations.

14 SOLAR ENERGY↗

DOE OSTI2024

Community Microgrid Assistance Partnership (C-MAP) [Slides]

This informational webinar, held on Nov. 11, 2024, covered the structure, technical focus, and eligibility criteria for the first Request for Proposals (RFP) released by the Community Microgrid Assistance Partnership (C-MAP). Eligible communities are invited to propose a community-based microgrid development or improvement project for funding and/or technical assistance. C-MAP is a program led by the U.S. Department of Energy Office of Electricity with management and coordination by the National Renewable Energy Laboratory. C-MAP will bring together many of the organizations that are working to understand, improve, and implement advanced microgrid energy systems in underserved and Indigenous communities in remote areas, facilitating a new forum for engagement and collaboration. Wraparound services from regional organizations will support the participating communities in microgrid operations as it relates to advancing their energy goals.

24 POWER TRANSMISSION AND DISTRIBUTION↗

DOE OSTI2024

DERiving Community Economic Development through Distributed Solar

This report explores how distributed solar stakeholders can plan and align economic development with existing community priorities while advancing new solar projects. Because the amount of local community influence drives community economic development, we note the need for investment in community ownership models, which are scarce today. This report seeks to outline the current barriers to community-led economic development through distributed solar, best practices for implementation, and recommendations for sector stakeholders.

14 SOLAR ENERGY↗

DOE OSTI2024

DERiving Community Economic Development through Distributed Solar

14 SOLAR ENERGY↗

DOE OSTI2013

The Epsomitic Phototrophic Microbial Mat of Hot Lake, Washington. Community Structural Responses to Seasonal Cycling

Phototrophic microbial mats are compact ecosystems composed of highly interactive organisms in which energy and element cycling take place over millimeter-to-centimeter-scale distances. Although microbial mats are common in hypersaline environments, they have not been extensively characterized in systems dominated by divalent ions. Hot Lake is a meromictic, epsomitic lake that occupies a small, endorheic basin in north-central Washington. The lake harbors a benthic, phototrophic mat that assembles each spring, disassembles each fall, and is subject to greater than tenfold variation in salinity (primarily Mg 2+ and SO 2 -4 ) and irradiation over the annual cycle. We examined spatiotemporal variation in the mat community at five time points throughout the annual cycle with respect to prevailing physicochemical parameters by amplicon sequencing of the V4 region of the 16S rRNA gene coupled to near-full-length 16S RNA clone sequences. The composition of these microbial communities was relatively stable over the seasonal cycle and included dominant populations of Cyanobacteria, primarily a group IV cyanobacterium (Leptolyngbya), and Alphaproteobacteria (specifically, members of Rhodobacteraceae and Geminicoccus). Members of Gammaproteobacteria (e.g., Thioalkalivibrio and Halochromatium) and Deltaproteobacteria (e.g., Desulfofustis) that are likely to be involved in sulfur cycling peaked in summer and declined significantly by mid-fall, mirroring larger trends in mat community richness and evenness. Phylogenetic turnover analysis of abundant phylotypes employing environmental metadata suggests that seasonal shifts in light variability exert a dominant influence on the composition of Hot Lake microbial mat communities. The seasonal development and organization of these structured microbial mats provide opportunities for analysis of the temporal and physical dynamics that feed back to community function.

16S tag sequencing↗

DOE OSTI2026

Sharing the Sun: Community Solar Deployment and Subscriptions (As of January 2026)

The community solar market analysis presented here is based primarily on data collected through Sharing the Sun, an initiative of the National Community Solar Partnership+ (NCSP+). Sharing the Sun data collection and analysis are conducted by the National Laboratory of the Rockies (NLR) as part of its support for implementation of NCSP+. NLR first released a dataset of community solar projects in 2018 and updates it biannually. The January 2026 dataset, data collection methodology, and all the previous datasets are available from NLR's Data Catalog: https://data.nlr.gov/submissions/244. The dataset presents project-level information including location, capacity, operating utility, and year of interconnection. The dataset is created from multiple data sources such as utility data, public utility commissions, project developer websites, media releases, primary data collection by NLR, and data provided by developers under nondisclosure agreements. This presentation builds on a previous analysis of the community solar project dataset, Sharing the Sun: Community Solar Deployment and Subscriptions (as of June 2024). Dr. Gabriel Chan and his team at the University of Minnesota contribute to this effort. NCSP+ is led and funded by U.S. Department of Energy's Integrated Energy Systems Office (IESO).

14 SOLAR ENERGY↗

DOE OSTI2015

Effects of Precommercial Thinning and Midstory Control on Avian and Small Mammal Communities during Longleaf Pine Savanna Restoration.

Abstract - Restoring longleaf pine (Pinus palustris Mill.) savanna is a goal of many southern land managers, and longleaf plantations may provide a mechanism for savanna restoration. However, the effects of silvicultural treatments used in the management of longleaf pine plantations on wildlife communities are relatively unknown. Beginning in 1994, we examined effects of longleaf pine restoration with plantation silviculture on avian and small mammal communities using four treatments in four 8- to 11- year-old plantations within the Savannah River Site in South Carolina. Treatments included prescribed burning every 3 to 5 years, plus: (1) no additional treatment (burn-only control); (2) precommercial thinning; (3) non-pine woody control with herbicides; and (4) combined thinning and woody control. We surveyed birds (1996-2003) using 50-m point counts and small mammals with removal trapping. Thinning and woody control alone had short-lived effects on avian communities, and the combination treatment increased avian parameters over the burn-only control in all years. Small mammal abundance showed similar trends as avian abundance for all three treatments when compared with the burn-only control, but only for 2 years post-treatment. Both avian and small mammal communities were temporarily enhanced by controlling woody vegetation with chemicals in addition to prescribed fire and thinning. Therefore, precommercial thinning in longleaf plantations, particularly when combined with woody control and prescribed fire, may benefit early-successional avian and small mammal communities by developing stand conditions more typical of natural longleaf stands maintained by periodic fire.

60 APPLIED LIFE SCIENCES↗

DOE OSTI2022

Intersections of Disadvantaged Communities and Renewable Energy Potential: Analyses to Inform Equitable Investment Prioritization

Renewable energy development can bolster local economies through job creation, local tax revenues, and reduced energy costs; however, communities most in need of economic development and employment opportunities often see lower levels of renewable energy deployment. Megan Day and Liz Ross, along with their co-authors and supported by NREL's Sustainable Communities Catalyzer, identified areas where disadvantaged community indicators intersect with high potential for renewable energy deployment. Through a geospatial intersection of energy burden, environmental hazard, and sociodemographic data with the technical generation potential and levelized cost of energy for multiple renewable energy technologies, we identified trends across disadvantaged community indicators and renewable energy deployment potential. Combining metrics across several tools, including the State and Local Planning for Energy (SLOPE) platform, the Low-Income Energy Affordability (LEAD) tool, and the Environmental Justice Screening and Mapping (EJSCREEN) tool, we compiled a dataset that can be used to inform national- and state-level energy-related assistance programs, economic development efforts, and infrastructure programs seeking to prioritize investments in disadvantage communities.

catalyzer↗