Engineering Papers⌕ Search

SEARCH · Engineering Papers

Results for “Er”

Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 559 records · Page 31
All sourcesNASA NTRSDOE OSTI
NASA NTRS1996

SUCCESS Studies of the Impact of Aircraft on Cirrus Clouds

During April of 1996 NASA will sponsor the SUCCESS project to better understand the impact of subsonic aircraft on the Earth's radiation budget. We plan to better determine the radiative properties of cirrus clouds and of contrails so that satellite observations can better determine their impact on Earth's radiation budget. We hope to determine how cirrus clouds form, whether the exhaust from subsonic aircraft presently affects the formation of cirrus clouds, and if the exhaust does affect the clouds whether the changes induced are of climatological significance. We seek to pave the way for future studies by developing and testing several new instruments. We also plan to better determine the characteristics of gaseous and particulate exhaust products from subsonic aircraft and their evolution in the region near the aircraft. In order to achieve our experimental objectives we plan to use the DC-8 aircraft as an in situ sampling platform. It will carry a wide variety of gaseous, particulate, radiative, and meteorological instruments. We will also use a T-39 aircraft primarily to sample the exhaust from other aircraft. It will carry a suite of instruments to measure particles and gases. We will employ an ER-2 aircraft as a remote sensing platform. The ER-2 will act as a surrogate satellite so that remote sensing observations can be related to the in situ parameters measured by the DC-8 and T-39. The mission strategy calls for a 5 week deployment beginning on April 8, 1996, and ending on May 10, 1996. During this time all three aircraft will be based in Salina, Kansas. A series of flights, averaging one every other day during this period, will be made mainly near the Department of Energy's Climate and Radiation Testbed site (CART) located in Northern Oklahoma, and Southern Kansas. During this same time period an extensive set of ground based measurements will be made by the DOE, which will also be operating several aircraft in the area to better understand the radiative properties of the atmosphere. Additional flights will be made over the Rocky Mountains, to investigate wave clouds. Flights will also be made over the Gulf of Mexico to utilize an oceanic background for remote sensing measurements. The results of this mission will be presented in this talk.

Toon, Owen B.↗
NASA NTRS1997

An Overview of the Tropospheric Aerosol Radiative Forcing Observational Experiment

Aerosol effects on atmospheric radiation are a leading source of uncertainty in predicting future climate. As a result, the International Global Atmospheric Chemistry Program has established a Focus on Atmospheric Aerosols (IGAC/FAA) and endorsed a series of aerosol field campaigns. TARFOX, the second in the IGAC/FAA series, was designed to reduce this uncertainty by measuring aerosol properties and effects in the US eastern seaboard, where one of the world's major plumes of industrial haze moves from the continent over the Atlantic Ocean. TARFOX's objectives are to: 1. Make simultaneous measurements of: (a) aerosol effects on radiation fields, and (b) the chemical, physical, and optical properties of the aerosols causing those effects. 2. Perform a variety of closure studies by using overdetermined data sets to test the mutual consistency of measurements and calculations of a wide range of aerosol properties and effects. 3. Use the results of the closure studies to assess and reduce uncertainties in estimates of aerosol radiative forcing, as well as to guide future field programs. An important subset of the closure studies is tests and improvements of algorithms used to derive aerosol properties and radiative effects from satellite measurements. The TARFOX Intensive Field Period (IFP) was conducted July 10-31, 1996. It included coordinated measurements from four satellites (GOES-8, NOAA-14, ERS-2, LANDSAT), four aircraft (ER-2, C-130, C-131, and a modified Cessna), land sites, and ships. A variety of aerosol conditions was sampled, ranging from relatively clean behind frontal passages to moderately polluted with aerosol optical depths exceeding 0.5 at mid-visible wavelengths. The latter conditions included separate incidents of enhancements caused primarily by anthropogenic sources and another incident of enhancement apparently influenced by recent fog processing. Spatial gradients of aerosol optical thickness were sampled to aid in isolating aerosol effects from other radiative effects and to more tightly constrain closure tests, including those of satellite retrievals. This talk gives an overview of TARFOX goals, rationale, methods, and initial key findings.

Russell, P. B.↗
NASA NTRS1998

Observations of Subvisible Cirrus Clouds and Gravity Waves at the Tropical Tropopause

Thin, subvisible cirrus (SVC) clouds at the tropical tropopause have been observed by a number of methods in a variety of observational programs, including in situ sampling and aircraft and space-based lidar. Modeling studies suggest that these clouds play an important role in dehydrating tropospheric air as it enters the stratosphere. This is because particles large enough to have significant fall speeds can form under the conditions of slow cooling that are implied by the large horizontal extent of the SVC sheets. The IR radiation that these clouds absorb, and the upward vertical motion this implies, also makes them candidates for a tropical troposphere-to-stratosphere mass transfer mechanism. They may also play a role in the earth's radiation budget. These sheets were observed on five flights during the Tropical Ozone Transport Experiment (TOTE) by the NASA Langley DIAL lidar aboard NASA's DC-8 research aircraft operating during December 1995 and February 1996 south of Hawaii. The relationship of the SVC's observed during TOTE to convection was not a simple one. One class of SVC's are within 1000 km of the persistent strong convection near 15S (the SPCZ). Trajectory analyses indicated that the SVC air masses have in fact passed through the SPCZ within a few days of observation. These clouds are very close to the tropopause, with maximum potential temperatures not much higher than 370K, consistent with in situ water and total water measurements near the tropopause made during the Stratosphere Troposphere Exchange Project in January 1987 at Darwin, Australia. A second class of SVC's are not immediately downstream of convection. These clouds tend to be higher, reaching potential temperatures of 390K or more. Trajectory analyses indicate that the air in these SVC's originates either in the equatorial western Pacific or along the subtropical jet. In any case, the warm temperatures the SVC air masses encounter just prior to the observation time along the back trajectory imply that the clouds cannot be residual particles from cirrus blowoff, but must form locally as the air move upward and equatorward south of Hawaii. Since all the parcels have encountered colder temperatures than those at the time of observation early in their history, subsynoptic scale temperatures colder than the analysis temperatures appear to be required to explain the formation of ice particles. In fact, the sloping shapes of the SVC's do suggest that they are gravity or inertia-gravity waves. In situ meteorological measurements made by the ER-2 within a day of the DC-8 remote lidar observations show a gravity wave structure near the equator with an estimated period of about 30 hours. This is sufficiently long to allow large particles to form and fall out (thus allowing dehydration). Other ER-2 flights south of Hawaii at other times of year show gravity and inertia-gravity waves with a poleward wavenumber component and significant (5 degrees peak to peak) temperature perturbation.

Pfister, Leonhard↗
NASA NTRS2001

Dynamical Interpolation of Mesoscale Flows in the TOPEX/ Poseidon Diamond Surrounding the U.S. Joint Global Ocean Flux Study Bermuda Atlantic Time-Series Study Site

An open boundary ocean model is configured in a domain bounded by the four TOPEX/Poseidon (TIP) ground tracks surrounding the U.S. Joint Global Ocean Flux Study Bermuda Atlantic Time-series Study (BATS) site. This implementation facilitates prescription of model boundary conditions directly from altimetric measurements (both TIP and ERS-2). The expected error characteristics for a domain of this size with periodically updated boundary conditions are established with idealized numerical experiments using simulated data. A hindcast simulation is then constructed using actual altimetric observations during the period October 1992 through September 1998. Quantitative evaluation of the simulation suggests significant skill. The correlation coefficient between predicted sea level anomaly and ERS observations in the model interior is 0.89; that for predicted versus observed dynamic height anomaly based on hydrography at the BATS site is 0.73. Comparison with the idealized experiments suggests that the main source of error in the hindcast is temporal undersampling of the boundary conditions. The hindcast simulation described herein provides a basis for retrospective analysis of BATS observations in the context of the mesoscale eddy field.

McGillicuddy, D. J.↗
NASA NTRS2002

Structure of the Highly Sheared Tropical Storm Chantal During CAMEX -4

On 20 August 2001 during the Convection and Moisture Experiment 4 (CAMEX-4) and NOAA Hurricane Field Program (HFP2001), the NASA high-altitude ER-2 and medium-altitude DC-8, and lower-altitude NOAA P3 aircraft conducted a coordinated Quantitative Precipitation Estimation (QPE) mission focused on convection in Tropical Storm Chantal. This storm first became a depression on 14 August, a tropical storm on 17 August, and it maintained maximum winds of about 65-70 mph during 19-20 August with minimum pressures ranging from 1008 mb on 19 August to 1001 mb late on 20 August. The storm was westward moving and was forecasted to intensify and landfall near the Yucatan-Belize border late on 20 August. Chanter failed to intensify and instead exhibited a highly sheared structure with an open low-level circulation and intense convection well to the northeast of this circulation center. The NASA ER-2 and DC-8 aircraft were closely coordinated with the NOAA P3 (NOAA-42). The NASA aircraft collected remote sensing and in situ data sets, while the P3 collected lower level in situ and radar data; both the DC-8 and P3 released 7 and 24 dropsondes, respectively. These aircraft measurements provided a unique opportunity to examine the structure of a sheared system and why it did not develop as forecasted a few days earlier. This paper will describe a preliminary study of the precipitation and wind structure provided by the NASA aircraft within the context of the NOAA P3 measurements.

Heymsfield, Gerald M.↗
NASA NTRS1998

Mesoscale Variability in SUCCESS Data

Analysis of meteorological, chemical and microphysical data from the airborne SUCCESS (SUbsonic aircraft Contrail and Cloud Effects Special Study) mission is reported. Careful analysis of the complex DC-8 flight pattern of May 2, 1996 reveals 19 linear flight segments within six main geographical areas, which we have analyzed. Significant mountain wave activity is revealed in the data from the MMS (Meteorology Measurement System) and MTP (Microwave Temperature Profiler) instruments on the DC-8, which resembles previous observations of mountain wave structures near Boulder, Colorado. Strong mountain-wave-induced upwelling downwind of the Rockies is noted. Turbulence is also noted in regions of the mountain wave consistent with overturning near the tropopause. Zonal winds recorded on the ER-2 are shown to be consistent with mountain wave breaking at or near critical levels in the stratosphere, consistent with the strong turbulence reported by the pilot during the ER-2 flight. These observations have been supported with spectral analyses and modeling studies. 'Postcasts' of mountain wave activity on May 2, 1996 using the Naval Research Laboratory Mountain Wave Forecast Model predicts both strong mountain wave activity near the tropopause and strong mountain-wave-induced turbulence in the stratosphere.

Eckermann, Stephen D.↗
NASA NTRS2003

Satellite-Based Stratospheric and Tropospheric Measurements: Determination of Global Ozone and Other Trace Species

This grant is an extension to our previous NASA Grant NAG5-3461, providing incremental funding to continue GOME (Global Ozone Monitoring Experiment) and SCIAMACHY (SCanning Imaging Absorption SpectroMeter for Atmospheric CHartographY) studies. This report summarizes research done under these grants through December 31, 2002. The research performed during this reporting period includes development and maintenance of scientific software for the GOME retrieval algorithms, consultation on operational software development for GOME, consultation and development for SCIAMACHY near-real-time (NRT) and off-line (OL) data products, and participation in initial SCIAMACHY validation studies. The Global Ozone Monitoring Experiment was successfully launched on the ERS-2 satellite on April 20, 1995, and remains working in normal fashion. SCIAMACHY was launched March 1, 2002 on the ESA Envisat satellite. Three GOME-2 instruments are now scheduled to fly on the Metop series of operational meteorological satellites (Eumetsat). K. Chance is a member of the reconstituted GOME Scientific Advisory Group, which will guide the GOME-2 program as well as the continuing ERS-2 GOME program.

Chance, Kelly↗
NASA NTRS2002

Evaluation of GEOSAT Follow-On Precise Orbit Ephemeris

The GEOSAT Follow-On spacecraft (GFO-l), launched in 1998, began continuous radar altimeter coverage of the oceans in 2000, and after an extensive series of calibration campaigns in 1999 and 2000 was finally accepted by the US Navy on November 29, 2000. By providing high quality altimeter data, GFO can supplement TOPEX/POSEIDON(T/P), ERS-1, and ERS-2 data, providing a different synoptic sampling of the oceans with its 17-day ground track repeat cycle. Altimeter analysis suggests GFO is capable of POSEIDON class altimetry, with orbit error the largest contributor to the GFO altimeter error budget. Satellite laser ranging (SLR), especially in combination with altimeter crossover data, offer the only means of determining high-quality precise orbits. SLR tracking is also augmented by Doppler (Tranet style) beacons. These data were used to tune the gravity field and macromodel (3D representation of the spacecraft geometry and surface properties). Beginning with January 2000 GSFC has produced a 2.5 year span of the Precision Orbit Ephemeris (POE) intended for use on the GFO GDR and for the Pathfinder Project. Using GFO GDR and IGDR altimeter data from NOAA, a two year span of POE orbits are evaluated with respect to the TOPEX orbits by adjusting the GFO data into the TOPEX frame with the help of TOPEX/GFO altimeter crossovers. A 2.5 year span of orbits are also evaluated using tracking data residual analysis, GFO altimeter crossover residual analysis, and direct orbit comparison. In an effort to improve the current POE orbit several ideas are considered, including using recent gravity models which include CHAMP data, the reduced-dynamic approach, and including TOPEX/GFO altimeter crossover data in the orbit solution.

Lemoine, Frank G.↗
NASA NTRS2003

University of Washington Airborne Studies in Support of the CLAMS-2001 Field Study

The main activity under this grant was participation in the Chesapeake Lighthouse and Aircraft Measurements for Satellites (CLAMS) field study from 10 July through 2 August 2001. The Cloud and Aerosol Research Group (CARG) from the University of Washington (UW) flew its Convair-580 research aircraft on thirteen occasions, for a total of 45 research flight hours, in support of CLAMS. Some of the main accomplishments of these flights were: 1) Aerosol and trace gas measurements and sunphotometer measurements of aerosol optical depth and column water vapor and ozone from close to Ocean surface to approx. 10,000 ft off Delmarva Peninsula on various occasions; 2) Measurements of aerosol properties on seven occasions beneath the Terra satellite, once beneath AVHRR, and five times beneath the ER-2 aircraft; 3) Measurements of aerosol properties in the vicinity of the (CERES instrumented) Chesapeake Bay lighthouse (COVE) on nine occasions; 4) Use of the NASA Goddard Cloud Absorption Radiometer (CAR) to obtain measurements of BRDF of the ocean surface on fifteen occasions and over Great Dismal Swamp on two occasions; 5) Measurements of aerosol properties over instrumented buoys 44014, 44004, and 41001. 6) On July 17 (a CLAMS 'Golden Day') six aircraft, including the Convair-580 and ER-2, were stacked above the Chesapeake Bay lighthouse under clear skies at the time of the Terra overpass.

Hobbs, Peter V.↗
NASA NTRS2002

Russian-US Partnership to Study the 23-km-diameter El'gygtgyn Impact Crater, Northeast Russia

El'gygytgyn crater, located within Eastern Siberia, is a Pliocene-aged (3.6 Ma), well-preserved impact crater with a rim diameter of roughly 23 km. The target rocks are a coherent assemblage of crystalline rocks ranging from andesite to basalt. At the time of impact the region was forested and the Arctic Ocean was nearly ice-free. A 15-km lake fills the center of the feature and water depths are approximately 175 m. Evidence of shock metamorphism, -- including coesite, fused mineral glasses, and planar deformation features in quartz -- has been reported. This feature is one of the youngest and best preserved complex craters on Earth. Because of its remote Arctic setting, however, El gygytgyn crater remains poorly investigated. The objectives of this three-year project are to establish and maintain a research partnership between scientists from Russia and the United States interested in the El gygytgyn crater. The principal institutions in the U.S. will be the Geophysical Institute, University of Alaska Fairbanks and the University of Massachusetts Amherst. The principal institution in Russia will be the North East Interdisciplinary Scientific Research Institute (NEISRI), which is the Far-East Branch of the Russian Academy of Science. Three science tasks are identified for the exchange program: (1) Evaluate impactite samples collected during previous field excursions for evidence of and level of shock deformation. (2) Build a high-resolution digital elevation model for the crater and its surroundings using interferometric synthetic aperture radar techniques on JERS-1, ERS-1, ERS-2, and/or RadarSat range-doppler data. (3) Gather all existing surface data available from Russian and U.S. institutions (DEM, remote sensing image data, field-based lithological and sample maps, and existing geophysical data) and assemble into a Geographic Information Systems database.

Sharpton, Virgil L.↗
NASA NTRS2002

Convective Bursts and Hot Towers Observed During CAMEX-3 and CAMEX-4

The role of convective bursts in the inner core regions of tropical storms has been if interest for many years with respect to their role in intensification. The structure of these convective bursts is best observed in satellite observations, but their structure has also been observed in some of the airborne radar studies over the past decade. Recently, a convective burst was documented during Hurricane Bonnie during NASA's Convection and Moisure Experiment-3 (CAMEX-3, 1998) conducted jointly with NOAA's Hurricane Field Program (Heymsfield et al., 2001). CAMEX-3 involved the NASA ER-2 high-altitude and DC-8 medium altitude aircraft instrumented with a variety of remote sensing instruments. The Bonnie convective burst was suggested to contribute intensification if ots warm core vased on radar measurements provided by the ER-2 Doppler Radar (EDOP). During the 2001 hurricane season, data sets form additional storms were collected during CAMEX-4. This paper documents the internal structure of convective bursts observed by EDOP and supporting measurements during foour storms (Bonnie, 1998; Georges, 1998; Chantal, 2001; Humberto, 2001) with respect to draft intensities, height profiles of reflectivity, location with respect to the storm center, occurence relative to the sotrm evolutjion, and upper level outlflows associated with the burst. These cases will be presented in the poster and will be compared with Bonnie's convective burst whic was associated with a strong subsiding current which appeared to contribute to the storm intensification. For one of the sotrms (Chantal), a very intense convective burst was nearly 100 km east of the low level circulation center and thus was infavorably located for intensifying the storm.

Heymsfield, Gerald↗
NASA NTRS2004

Tropical Cyclone Precipitation Types and Electrical Field Information Observed by High Altitude Aircraft Instrumentation

During the 1998 and 200 1 hurricane seasons of the Atlantic Ocean Basin, the Advanced Microwave Precipitation Radiometer (AMPR), the ER-2 Doppler (EDOP) radar, and the Lightning Instrument Package (LIP) were flown aboard the National Aeronautics and Space Administration (NASA) ER-2 high altitude aircraft as part of the Third Convection And Moisture Experiment (CAMEX-3) and the Fourth Convection And Moisture Experiment (CAMEX-4). Several hurricanes and tropical storms were sampled during these experiments. A rainfall screening technique has been developed using AMPR passive microwave observations of these tropical cyclones (TC) collected at frequencies of 10.7, 19.35,37.1, and 85.5 GHz and verified using vertical profiles of EDOP reflectivity and lower altitude horizontal reflectivity scam collected by the National Oceanic and Atmospheric Administration (NOM) P-3 radar. Matching the rainfall classification results with coincident electrical field information collected by the LIP readily identifl convective rain regions within the TC precipitation fields. Strengths and weaknesses of the rainfall classification procedure will be discussed as well as its potential as a real-time analysis tool for monitoring vertical updrafl strength and convective intensity from a remotely operated or uninhabited aerial vehicle.

Hood, Robbie E.↗
NASA NTRS2004

Classification of Particle Shapes from Lidar Depolarization Ratios in Convective Ice Clouds Compared to in situ Observations During CRYSTAL-FACE

This manuscript describes a method to class@ cirrus cloud ice particle shape using lidar depolarization measurements as a basis for segregating different particle shape regimes. Measurements from the ER-2 Cloud Physics Lidar (CPL) system during CRYSTAL-FACE provide the basis for this work. While the CPL onboard the ER-2 aircraft was providing remote sensing measurements of cirrus clouds, the Cloud Particle Imager (CPI) onboard the WB-57 aircraft was flying inside those same clouds to sample particle sizes. The results of classifying particle shapes using the CPL data are compared to the in situ measurements made using the CPI , and there is found to be good agreement between the particle shape inferred from the CPL data and that actually measured by the CPI. If proven practical, application of this technique to spaceborne observations could lead to large-scale classification of cirrus cloud particle shapes.

Noel, Vincent↗
NASA NTRS2004

Classification of Tropical Oceanic Precipitation using High Altitude Aircraft: Microwave and Electric Field Measurements

During the 1998 and 2001 hurricane seasons of the western Atlantic Ocean and Gulf of Mexico, the Advanced Microwave Precipitation Radiometer (AMPR), the ER-2 Doppler (EDOP) radar, and the Lightning Instrument Package (LIP) were flown aboard the National Aeronautics and Space Administration ER-2 high altitude aircraft as part of the Third Convection and Moisture Experiment (CAMEX-3) and the Fourth Convection and Moisture Experiment (CAMEX-4). Several hurricanes, tropical storms, and other precipitation systems were sampled during these experiments. An oceanic rainfall screening technique has been developed using AMPR passive microwave observations of these systems collected at frequencies of 10.7, 19.35,37.1, and 85.5 GHz. This technique combines the information content of the four AMPR frequencies regarding the gross vertical structure of hydrometeors into an intuitive and easily executable precipitation mapping format. The results have been verified using vertical profiles of EDOP reflectivity and lower altitude horizontal reflectivity scans collected by the National Oceanic and Atmospheric Administration WP-3D Orion radar. Matching the rainfall classification results with coincident electric field information collected by the LIP readily identifies convective rain regions within the precipitation fields. This technique shows promise as a real-time research and analysis tool for monitoring vertical updraft strength and convective intensity from airborne platforms such as remotely operated or uninhabited aerial vehicles. The technique is analyzed and discussed for a wide variety of precipitation types using the 26 August 1998 observations of Hurricane Bonnie near landfall.

Hood, Robbie E.↗
NASA NTRS2001

Updated Electronic Testbed System

As we continue to advance in exploring space frontiers, technology must also advance. The need for faster data recovery and data processing is crucial. In this, the less equipment used, and lighter that equipment is, the better. Because integrated circuits become more sensitive in high altitude, experimental verification and quantification is required. The Center for Applied Radiation Research (CARR) at Prairie View A&M University was awarded a grant by NASA to participate in the NASA ER-2 Flight Program, the APEX balloon flight program, and the Student Launch Program. These programs are to test anomalous errors in integrated circuits due to single event effects (SEE). CARR had already begun experiments characterizing the SEE behavior of high speed and high density SRAM's. The research center built a error testing system using a PC-104 computer unit, an Iomega Zip drive for storage, a test board with the components under test, and a latchup detection and reset unit. A test program was written to continuously monitor a stored data pattern in the SRAM chip and record errors. The devices under test were eight 4Mbit memory chips totaling 4Mbytes of memory. CARR was successful at obtaining data using the Electronic TestBed System (EBS) in various NASA ER-2 test flights. These series of high altitude flights of up to 70,000 feet, were effective at yielding the conditions which single event effects usually occur. However, the data received from the series of flights indicated one error per twenty-four hours. Because flight test time is very expensive, the initial design proved not to be cost effective. The need for orders of magnitude with more memory became essential. Therefore, a project which could test more memory within a given time was created. The goal of this project was not only to test more memory within a given time, but also to have a system with a faster processing speed, and which used less peripherals. This paper will describe procedures used to build an updated Electronic Testbed System.

Brewer, Kevin L.↗
NASA NTRS2003

Increased Agrobacterium-mediated transformation and rooting efficiencies in canola (Brassica napus L.) from hypocotyl segment explants

An efficient protocol for the production of transgenic Brassica napus cv. Westar plants was developed by optimizing two important parameters: preconditioning time and co-cultivation time. Agrobacterium tumefaciens-mediated transformation was performed using hypocotyls as explant tissue. Two variants of a green fluorescent protein (GFP)-encoding gene--mGFP5-ER and eGFP--both under the constitutive expression of the cauliflower mosaic virus 35S promoter, were used for the experiments. Optimizing the preconditioning time to 72 h and co-cultivation time with Agrobacterium to 48 h provided the increase in the transformation efficiency from a baseline of 4% to 25%. With mGFP5-ER, the transformation rate was 17% and with eGFP it was 25%. Transgenic shoots were selected on 200 mg/l kanamycin. Rooting efficiency was 100% on half-strength Murashige and Skoog medium with 10 g/l sucrose and 0.5 mg/l indole butyric acid in the presence of kanamycin.

NASA Discipline Life Sciences Technologies↗
NASA NTRS2003

Elevated levels of N-lauroylethanolamine, an endogenous constituent of desiccated seeds, disrupt normal root development in Arabidopsis thaliana seedlings

N-Acylethanolamines (NAEs) are prevalent in desiccated seeds of various plant species, and their levels decline substantially during seed imbibition and germination. Here, seeds of Arabidopsis thaliana (L.) Heynh. were germinated in, and seedlings maintained on, micromolar concentrations of N-lauroylethanolamine (NAE 12:0). NAE 12:0 inhibited root elongation, increased radial swelling of root tips, and reduced root hair numbers in a highly selective and concentration-dependent manner. These effects were reversible when seedlings were transferred to NAE-free medium. Older seedlings (14 days old) acclimated to exogenous NAE by increased formation of lateral roots, and generally, these lateral roots did not exhibit the severe symptoms observed in primary roots. Cells of NAE-treated primary roots were swollen and irregular in shape, and in many cases showed evidence, at the light- and electron-microscope levels, of improper cell wall formation. Microtubule arrangement was disrupted in severely distorted cells close to the root tip, and endoplasmic reticulum (ER)-localized green fluorescent protein (mGFP5-ER) was more abundant, aggregated and distributed differently in NAE-treated root cells, suggesting disruption of proper cell division, endomembrane organization and vesicle trafficking. These results suggest that NAE 12:0 likely influences normal cell expansion in roots by interfering with intracellular membrane trafficking to and/or from the cell surface. The rapid metabolism of NAEs during seed imbibition/germination may be a mechanism to remove this endogenous class of lipid mediators to allow for synchronized membrane reorganization associated with cell expansion.

Non-NASA Center↗
NASA NTRS2000

Diversity and regulation of plant Ca2+ pumps: insights from expression in yeast

The spatial and temporal regulation of calcium concentration in plant cells depends on the coordinate activities of channels and active transporters located on different organelles and membranes. Several Ca2+ pumps have been identified and characterized by functional expression of plant genes in a yeast mutant (K616). This expression system has opened the way to a genetic and biochemical characterization of the regulatory and catalytic features of diverse Ca2+ pumps. Plant Ca(2+)-ATPases fall into two major types: AtECA1 represents one of four or more members of the type IIA (ER-type) Ca(2+)-ATPases in Arabidopsis, and AtACA2 is one of seven or more members of the type IIB (PM-type) Ca(2+)-ATPases that are regulated by a novel amino terminal domain. Type IIB pumps are widely distributed on membranes, including the PM (plasma membrane), vacuole, and ER (endoplasmic reticulum). The regulatory domain serves multiple functions, including autoinhibition, calmodulin binding, and sites for modification by phosphorylation. This domain, however, is considerably diverse among several type IIB ATPases, suggesting that the pumps are differentially regulated. Understanding of Ca2+ transporters at the molecular level is providing insights into their roles in signaling networks and in regulating fundamental processes of cell biology.

Review, Academic↗