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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 559 records · Page 31
All sourcesNASA NTRSDOE OSTI
NASA NTRS1995

Template switching between PNA and RNA oligonucleotides

The origin of the RNA world is not easily understood, as effective prebiotic syntheses of the components of RNA, the beta-ribofuranoside-5'-phosphates, are hard to envisage. Recognition of this difficulty has led to the proposal that other genetic systems, the components of which are more easily formed, may have preceded RNA. This raises the question of how transitions between one genetic system and another could occur. Peptide nucleic acid (PNA) resembles RNA in its ability to form double-helical complexes stabilized by Watson-Crick hydrogen bonding between adenine and thymine and between cytosine and guanine, but has a backbone that is held together by amide rather than by phosphodiester bonds. Oligonucleotides bases on RNA are known to act as templates that catalyse the non-enzymatic synthesis of their complements from activated mononucleotides, we now show that RNA oligonucleotides facilitate the synthesis of complementary PNA strands and vice versa. This suggests that a transition between different genetic systems can occur without loss of information.

NASA Discipline Exobiology↗
NASA NTRS1995

Asymmetric structure of five and six membered DNA hairpin loops

The tertiary structure of nucleic acid hairpins was elucidated by means of the accessibility of the single-strand-specific nuclease from mung bean. This molecular probe has proven especially useful in determining details of the structural arrangement of the nucleotides within a loop. In this study 3'-labeling is introduced to complement previously used 5'-labeling in order to assess and to exclude possible artifacts of the method. Both labeling procedures result in mutually consistent cleavage patterns. Therefore, methodological artifacts can be excluded and the potential of the nuclease as structural probe is increased. DNA hairpins with five and six membered loops reveal an asymmetric loop structure with a sharp bend of the phosphate-ribose backbone between the second and third nucleotide on the 3'-side of a loop. These hairpin structures differ from smaller loops with 3 or 4 members, which reveal this type of bend between the first and second 3' nucleotide, and resemble with respect to the asymmetry anticodon loops of tRNA.

NASA Discipline Exobiology↗
NASA NTRS1994

The beetle Tribolium castaneum has a fushi tarazu homolog expressed in stripes during segmentation

The genetic control of embryonic organization is far better understood for the fruit fly Drosophila melanogaster than for any other metazoan. A gene hierarchy acts during oogenesis and embryogenesis to regulate the establishment of segmentation along the anterior-posterior axis, and homeotic selector genes define developmental commitments within each parasegmental unit delineated. One of the most intensively studied Drosophila segmentation genes is fushi tarazu (ftz), a pair-rule gene expressed in stripes that is important for the establishment of the parasegmental boundaries. Although ftz is flanked by homeotic selector genes conserved throughout the metazoa, there is no evidence that it was part of the ancestral homeotic complex, and it has been unclear when the gene arose and acquired a role in segmentation. We show here that the beetle Tribolium castaneum has a ftz homolog located in its Homeotic complex and expressed in a pair-rule fashion, albeit in a register differing from that of the fly gene. These and other observations demonstrate that a ftz gene preexisted the radiation of holometabolous insects and suggest that it has a role in beetle embryogenesis which differs somewhat from that described in flies.

NASA Discipline Developmental Biology↗
NASA NTRS1994

Inventing and improving ribozyme function: rational design versus iterative selection methods

Two major strategies for generating novel biological catalysts exist. One relies on our knowledge of biopolymer structure and function to aid in the 'rational design' of new enzymes. The other, often called 'irrational design', aims to generate new catalysts, in the absence of detailed physicochemical knowledge, by using selection methods to search a library of molecules for functional variants. Both strategies have been applied, with considerable success, to the remodeling of existing ribozymes and the development of ribozymes with novel catalytic function. The two strategies are by no means mutually exclusive, and are best applied in a complementary fashion to obtain ribozymes with the desired catalytic properties.

Review, Tutorial↗
NASA NTRS1994

Purification of recombinant budgerigar fledgling disease virus VP1 capsid protein and its ability for in vitro capsid assembly

A recombinant system for the major capsid VP1 protein of budgerigar fledgling disease virus has been established. The VP1 gene was inserted into a truncated form of the pFlag-1 vector and expressed in Escherichia coli. The budgerigar fledgling disease virus VP1 protein was purified to near homogeneity by immunoaffinity chromatography. Fractions containing highly purified VP1 were pooled and found to constitute 3.3% of the original E. coli-expressed VP1 protein. Electron microscopy revealed that the VP1 protein was isolated as pentameric capsomeres. Electron microscopy also revealed that capsid-like particles were formed in vitro from purified VP1 capsomeres with the addition of Ca2+ ions and the removal of chelating and reducing agents.

NASA Discipline Developmental Biology↗
NASA NTRS1993

Phenotypic characterization of xpr, a global regulator of extracellular virulence factors in Staphylococcus aureus

We recently described a Tn551 insertion in the chromosome of Staphylococcus aureus S6C that resulted in drastically reduced expression of extracellular lipase (M. S. Smeltzer, S. R. Gill, and J. J. Iandolo, J. Bacteriol. 174:4000-4006, 1992). The insertion was localized to a chromosomal site (designated omega 1058) distinct from the lipase structural gene (geh) and the accessory gene regulator (agr), both of which were structurally intact in the lipase-negative (Lip-) mutants. In this report, we describe a phenotypic comparison between strains S6C, a hyperproducer of enterotoxin B; KSI9051, a derivative of S6C carrying the Tn551 insertion at omega 1058; ISP546, an 8325-4 strain that carries a Tn551 insertion in the agr locus; and ISP479C, the parent strain of ISP546 cured of the Tn551 delivery plasmid pI258repA36. Compared with their respective parent strains, ISP546 and KSI9051 produced greatly reduced amounts of lipase, alpha-toxin, delta-toxin, protease, and nuclease. KSI9051 also produced reduced amounts of staphylococcal enterotoxin B. Coagulase production was increased in ISP546 but not in KSI9051. Using a mouse model, we also demonstrated that ISP546 and KSI9051 were far less virulent than ISP479C and S6C. We have designated the genetic element defined by the Tn551 insertion at omega 1058 xpr to denote its role as a regulator of extracellular protein synthesis. We conclude that xpr and agr are similar and possibly interactive regulatory genes that play an important role in pathogenesis of staphylococcal disease.

NASA Discipline Cell Biology↗
NASA NTRS1992

Identification of a mouse B-type cyclin which exhibits developmentally regulated expression in the germ line

To begin to examine the function of cyclins in mammalian germ cells, we have screened an adult mouse testis cDNA library for the presence of B-type cyclins. We have isolated cDNAs that encode a murine B-type cyclin, which has been designated cycB1. cycB1 was shown to be expressed in several adult tissues and in the midgestation mouse embryo. In the adult tissues, the highest levels of cycB1 transcripts were seen in the testis and ovary, which contain germ cells at various stages of differentiation. The major transcripts corresponding to cycB1 are 1.7 and 2.5 kb, with the 1.7 kb species being the predominant testicular transcript and the 2.5 kb species more abundant in the ovary. Examination of cDNAs corresponding to the 2.5 kb and 1.7 kb mRNAs revealed that these transcripts encode identical proteins, differing only in the polyadenylation signal used and therefore in the length of their 3' untranslated regions. Northern blot and in situ hybridization analyses revealed that the predominant sites of cycB1 expression in the testis and ovary were in the germinal compartment, particularly in early round spermatids in the testis and growing oocytes in the ovary. Thus cycB1 is expressed in both meiotic and postmeiotic cells. This pattern of cycB1 expression further suggests that cycB1 may have different functions in the two cell types, only one of which correlates with progression of the cell cycle.

NASA Discipline Developmental Biology↗
NASA NTRS2004

Expression of gibberellin 3 beta-hydroxylase gene in a gravi-response mutant, weeping Japanese flowering cherry

Expressions of the gibberellin biosynthesis gene were investigated in a normal upright type and a gravi-response mutant, a weeping type of Japanese flowering cherry (Prunus spachiana), that is unable to support its own weight and elongates downward. A segment of the gibberellin 3 beta-hydroxylase cDNA of Prunus spachiana (Ps3ox), which is responsible for active gibberellin synthesis, was amplified by using real-time RT-PCR. The content of Ps3ox mRNA in the weeping type was much greater than that in the upright type, while the endogenous gibberellin level was much higher in the elongating zone of the weeping type. These results suggest that the amount and distribution of synthesized gibberellin regulate secondary xylem formation, and the unbalanced distribution of gibberellin affects the gravi-response of the Prunus tree.

Gravitropism/physiology↗
NASA NTRS2008

Spectroscopic Confirmation of a Massive Red-sequence Selected Galaxy Cluster at Z=1.34 in the SpARCS-South Cluster Survey

The Spitzer Adaptation of the Red-sequence Cluster Survey (SpARCS) is a z'-passband imaging survey, consisting of deep (z' approx. 24 AB) observations made from both hemispheres using the CFHT 3.6m and CTIO 4m telescopes. The survey was designed with the primary aim of detecting galaxy clusters at z > 1. In tandem with pre-existing 3.6 micron observations from the Spitzer Space Telescope SWIRE Legacy Survey, SpARCS detects clusters using an infrared adaptation of the two-filter red-sequence cluster technique. The total effective area of the SpARCS cluster survey is 41.9 sq deg. In this paper, we provide an overview of the 13.6 sq deg Southern CTIO/MOSAICII observations. The 28.3 sq deg Northern CFHT/MegaCam observations are summarized in a companion paper by Muzzin et al. (2008a). In this paper, we also report spectroscopic confirmation of SpARCS J003550-431224, a very rich galaxy cluster at z = 1.335, discovered in the ELAIS-S1 field. To date, this is the highest spectroscopically confirmed redshift for a galaxy cluster discovered using the red-sequence technique. Based on nine confirmed members, SpARCS J003550-431224 has a preliminary velocity dispersion of 1050+/-230 km/s. With its proven capability for efficient cluster detection, SpARCS is a demonstration that we have entered an era of large, homogeneously-selected z > 1 cluster surveys.

Wilson, Gillian↗
NASA NTRS2010

Seagrass Health Modeling and Prediction with NASA Science Data

Previous research has demonstrated that MODIS data products can be used as inputs into the seagrass productivity model developed by Fong and Harwell (1994). To further explore this use to predict seagrass productivity, Moderate Resolution Imaging Spectroradiometer (MODIS) custom data products, including Sea Surface Temperature, Light Attenuation, and Chlorophyll-a have been created for use as model parameter inputs. Coastal researchers can use these MODIS data products and model results in conjunction with historical and daily assessment of seagrass conditions to assess variables that affect the productivity of the seagrass beds. Current monitoring practices involve manual data collection (typically on a quarterly basis) and the data is often insufficient for evaluating the dynamic events that influence seagrass beds. As part of a NASA-funded research grant, the University of Mississippi, is working with researchers at NASA and Radiance Technologies to develop methods to deliver MODIS derived model output for the northern Gulf of Mexico (GOM) to coastal and environmental managers. The result of the project will be a data portal that provides access to MODIS data products and model results from the past 5 years, that includes an automated process to incorporate new data as it becomes available. All model parameters and final output will be available through the use National Oceanic and Atmospheric Administration?s (NOAA) Environmental Research Divisions Data Access Program (ERDDAP) tools as well as viewable using Thematic Realtime Environmental Distributed Data Services (THREDDS) and the Integrated Data Viewer (IDV). These tools provide the ability to create raster-based time sequences of model output and parameters as well as create graphs of model parameters versus time. This tool will provide researchers and coastal managers the ability to analyze the model inputs so that the factors influencing a change in seagrass productivity can be determined over time.

Robinson, Harold D.↗
NASA NTRS2019

Polarimetric Retrievals of Cloud Droplet Number Concentrations

Cloud droplet number concentration (Nd) is an important parameter of liquid clouds and is crucial to understanding aerosol-cloud interactions. It couples boundary layer aerosol composition, size and concentration with cloud reflectivity. It affects cloud evolution, precipitation, radiative forcing, global climate and, through observation, can be used to partially monitor the first indirect effect. With its unique combination of multi-wavelength, multi-angle, total and polarized reflectance measurements, the Research Scanning Polarimeter (RSP) retrieves Nd with relatively few assumptions. The approach involves measuring cloud optical thickness, mean droplet extinction cross-section and cloud physical thickness. Polarimetric observations are capable of measuring the effective variance, or width, of the droplet size distribution. Estimating cloud geometrical thickness is also an important component of the polarimetric Nd retrieval, which is accomplished using polarimetric measurements in a water vapor absorption band to retrieve the amount of in-cloud water vapor and relating this to physical thickness. We highlight the unique abilities and quantify uncertainties of the polarimetric approach. We validate the approach using observational data from the North Atlantic and Marine Ecosystems Study (NAAMES). NAAMES targets specific phases in the seasonal phytoplankton lifecycle and ocean-atmosphere linkages. This study provides an excellent opportunity for the RSP to evaluate its approach of sensing Nd over a range of concentrations and cloud types with in situ measurements from a Cloud Droplet Probe (CDP). The RSP and CDP, along with an array of other instruments, are flown on the NASA C-130 aircraft, which flies in situ and remote sensing legs in sequence. Cloud base heights retrieved by the RSP compare well with those derived in situ (R=0.83) and by a ceilometer aboard the R.V. Atlantis (R=0.79). Comparing geometric mean values from 12 science flights throughout the NAAMES-1 and NAAMES-2 campaigns, we find a strong correlation between Nd retrieved by the RSP and CDP (R=0.96). A linear least squares fit has a slope of 0.92 and an intercept of 0.3 cm−3. Uncertainty in this comparison can be attributed to cloud 3D effects, nonlinear liquid water profiles, multilayered clouds, measurement uncertainty, variation in spatial and temporal sampling, and assumptions used within the method. Radiometric uncertainties of the RSP measurements lead to biases on derived optical thickness and cloud physical thickness, but these biases largely cancel out when deriving Nd for most conditions and geometries. We find that a polarimetric approach to sensing Nd is viable and the RSP is capable of accurately retrieving Nd for a variety of cloud types and meteorological conditions.

Droplet concentration↗
NASA NTRSDate not supplied

Building a Real-Time Flood Prediction Model for Improving Early Warning Systems in Ellicott City, Maryland

As flood events in the United States grow in frequency and intensity, the use of applied remote sensing analyses is increasingly necessary for effective flood monitoring and warning systems. The NASA DEVELOP National Program partnered with the local government of Howard County, Maryland, to investigate the use of machine learning for advanced flood risk detection, and to test the feasibility of integrating this approach into the county’s flood early warning system. To strengthen the efforts of the Howard County Office of Emergency Management (OEM), the project developed a statistical model capable of hindcasting the two severe flash flood events that devastated Ellicott City and transitioned to a ‘Long Short-Term Memory’ based sequence-to-sequence deep learning model with 8-hour forecast capability. The team combined data inputs from public sources including river and precipitation gauges, NASA and NOAA Earth observations, and numerical weather model products using scripts written in the Google Colaboratory Python scripting environment. In addition to designing the deep learning architecture, the team trained and tested the model, and evaluated its performance using Nash-Sutcliffe Efficiency. The final product, the Sequentially Trained Real-time EstimAted Model (STREAM) predicts stage height for the Hudson Branch gauge in Ellicott City using data products available in near real-time, including the High-Resolution Rapid Refresh model’s accumulated precipitation forecasts supplemented by stream gauge data from the OEM and the U.S. Geological Survey. STREAM was incorporated into an online dashboard in a user-friendly interface capable of triggering the alarms that initiate emergency response protocols up to 8 hours in advance of a predicted severe flood event. The project demonstrated the potential for the integration of open data and Earth observations into a flood risk forecasting tool capable of informing near real-time decision making.

NASA DEVELOP↗
NASA NTRSDate not supplied

Building a Real-Time Predictive Flood Model for Improving Early Warning Systems in Ellicott City, Maryland

As flood events in the United States grow in frequency and intensity, the use of applied remote sensing analyses is increasingly necessary for effective flood monitoring and warning systems. The NASA DEVELOP National Program partnered with the Howard County government in Maryland to investigate the use of machine learning for advanced flood risk detection, and to test the feasibility of integrating this approach into the county’s flood early warning system. To strengthen the efforts of the Howard County Office of Emergency Management (OEM), the project developed a prediction model capable of hindcasting the two severe flash flood events that devastated Ellicott City, and transitioned to an Long Short-Term Memory (LSTM) based sequence-to-sequence deep learning model with 8-hour forecast capability. The team combined data inputs from public sources including river and precipitation gauges, NASA and NOAA Earth observations, and numerical weather model products using scripts written in the Google Colaboratory Python scripting environment. In addition to designing the deep learning architecture, the team trained and tested the model, and evaluated its performance using the Nash-Sutcliffe Efficiency (NSE). The final product, called the Sequentially Trained Real-time EstimAted Model (STREAM), predicts stage height for the Hudson Branch gauge in Ellicott City using data products available in near real-time, including the High-Resolution Rapid Refresh (HRRR) model’s accumulated precipitation forecasts supplemented by stream gauge data from the OEM and the U.S. Geological Survey. STREAM was incorporated into an online dashboard in a user-friendly interface capable of triggering the alarms that initiate the OEM’s emergency response protocols up to 8 hours in advance of a predicted severe flood event. The project demonstrated the potential for the integration of open data and Earth observations into a flood risk forecasting tool capable of informing near real-time decision making.

Ryan Hammock↗
DOE OSTI2018

Innovative nanopore sequencing technology including a self-assembled porous membrane

Methods and apparatus for long read, label-free, optical nanopore long chain molecule sequencing. In general, the present disclosure describes a novel sequencing technology based on the integration of nanochannels to deliver single long-chain molecules with widely spaced (>wavelength), ˜1-nm aperture “tortuous” nanopores that slow translocation sufficiently to provide massively parallel, single base resolution using optical techniques. A novel, directed self-assembly nanofabrication scheme using simple colloidal nanoparticles is used to form the nanopore arrays atop nanochannels that unfold the long chain molecules. At the surface of the nanoparticle array, strongly localized electromagnetic fields in engineered plasmonic/polaritonic structures allow for single base resolution using optical techniques.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
DOE OSTI2019

Temporal data system

A method for processing data is provided. Data is identified by a computer system. The data is processed in parallel by the computer system using temporal transformations to form pieces of temporal data. The pieces of temporal data are placed by the computer system in an order as the pieces of temporal data are generated by the temporal transformations to form a sequence of temporal data. The order of the sequence is based on a priority of when the pieces of temporal data should be processed, enabling performing an action.

Naegle, John H.↗