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NASA NTRS1979

Soft X-ray observations of degenerate dwarfs and cataclysmic variables

Observations in outburst of two dwarf novae, SS Cyg and U Gem, reveal soft, pulsed X-ray emission greatly enhanced over the emission present during the quiescent periods. In order to characterize the nature of the X-ray pulsations a random phase analysis was carried out, which indicated the phase of SS Cyg behaved like a random variable with zero mean and an r.m.s. value that increased with the square root of the time. Attention is also given to the properties of the detected dwarf novae during optical quiescences, to isolated white dwarfs, and to optical observations of AM Her. It is concluded that the dwarf novae, which occur in binary systems, appear to be controlled by the varying accretion of matter generated by the main sequence companion, and that the X-ray emission during outburst appears to consist of two separate components: a periodically varying (on about a 10 s timescale) soft component, together with a more slowly randomly varying hard component.

Garmire, G.↗

NASA NTRS1993

Preliminary report of lunar observations by the Near-Infrared Mapping Spectrometer (NIMS) during the second Galileo Earth-Moon encounter

The Galileo encounter with the Earth-Moon system on 8 Dec. 1992, provided a unique opportunity to observe the Moon. Galileo's closest approach to the Moon was at an altitude of about 110,000 km above an area at about latitude 60N, longitude 60E. During the 12 hours surrounding the time of lunar closest approach, 12 observational sequences were executed by the near-infrared mapping spectrometer (NIMS), many in coordination with other Galileo instruments. These NIMS observations provided nearly complete coverage of the illuminated crescent from phase angles of 123 deg to 14 deg. This phase-angle coverage with nearly constant illumination is not possible from Earth; it is expected to provide substantial new information on the nature of the lunar photometric function over wavelength. As of Jan. 1993, most of the analysis has focused on the highest resolution data (55 km/pixel).

Carlson, R. W.↗

NASA NTRS1993

On the estimation of the correlation dimension and its application to radar reflector discrimination

Recently, system theorists have recognized that low order systems of nonlinear differential equations can give rise to solutions which are neither periodic, constant, nor predictable in steady state, but which are nonetheless bounded and deterministic. This behavior, which was first described in the study of weather systems, has been termed 'chaotic.' Much study of chaotic systems has concentrated on analysis of the systems' phase space attractors. It has been recognized that invariant measures of the attractor possess inherent information about the system. One such measure is the dimension of the attractors. The dimension of a chaotic attractor has been shown to be noninteger, leading to the term 'strange attractor;' the attractor is said to have a fractal structure. The correlation dimension has become one of the most popular measures of dimension. However, many problems have been identified in correlation dimension estimation from time sequences. The most common methods for obtaining the correlation dimension have been least squares curves fitting to find the slope of the correlation integral and the Takens Estimator. However, these estimates show unacceptable sensitivity to the upper limit on the distance chosen. Here, a new method is proposed which is shown to be rather insensitive to the upper limit and to perform in a very stable manner, at least in the absence of noise. The correlation dimension is also shown to be an effective discriminant in distinguishing between radar returns resulting from weather and those from the ground. The weather returns are shown to have a correlation dimension generally between 2.0 and 3.0, while ground returns have a correlation dimension exceeding 3.0.

Barnett, Kevin D.↗

NASA NTRS1995

Thermal behavior spiral bevel gears

An experimental and analytical study of the thermal behavior of spiral bevel gears is presented. Experimental data were taken using thermocoupled test hardware and an infrared microscope. Many operational parameters were varied to investigate their effects on the thermal behavior. The data taken were also used to validate the boundary conditions applied to the analytical model. A finite element-based solution sequence was developed. The three-dimensional model was developed based on the manufacturing process for these gears. Contact between the meshing gears was found using tooth contact analysis to describe the location, curvatures, orientations, and surface velocities. This information was then used in a three-dimensional Hertzian contact analysis to predict contact ellipse size and maximum pressure. From these results, an estimate of the heat flux magnitude and the location on the finite element model was made. The finite element model used time-averaged boundary conditions to permit the solution to attain steady state in a computationally efficient manner.Then time- and position-varying boundary conditions were applied to the model to analyze the cyclic heating and cooling due to the gears meshing and transferring heat to the surroundings, respectively. The model was run in this mode until the temperature behavior stabilized. The transient flash temperature on the surface was therefore described. The analysis can be used to predict the overall expected thermal behavior of spiral bevel gears. The experimental and analytical results were compared for this study and also with a limited number of other studies. The experimental and analytical results attained in the current study were basically within 10% of each other for the cases compared. The experimental comparison was for bulk thermocouple locations and data taken with an infrared microscope. The results of a limited number of other studies were compared with those obtained herein and predicted the same basic behavior.

Handschuh, Robert F.↗

NASA NTRS2013

An Alternative Flight Software Trigger Paradigm: Applying Multivariate Logistic Regression to Sense Trigger Conditions Using Inaccurate or Scarce Information

In late 2014, NASA will fly the Orion capsule on a Delta IV-Heavy rocket for the Exploration Flight Test-1 (EFT-1) mission. For EFT-1, the Orion capsule will be flying with a new GPS receiver and new navigation software. Given the experimental nature of the flight, the flight software must be robust to the loss of GPS measurements. Once the high-speed entry is complete, the drogue parachutes must be deployed within the proper conditions to stabilize the vehicle prior to deploying the main parachutes. When GPS is available in nominal operations, the vehicle will deploy the drogue parachutes based on an altitude trigger. However, when GPS is unavailable, the navigated altitude errors become excessively large, driving the need for a backup barometric altimeter to improve altitude knowledge. In order to increase overall robustness, the vehicle also has an alternate method of triggering the parachute deployment sequence based on planet-relative velocity if both the GPS and the barometric altimeter fail. However, this backup trigger results in large altitude errors relative to the targeted altitude. Motivated by this challenge, this paper demonstrates how logistic regression may be employed to semi-automatically generate robust triggers based on statistical analysis. Logistic regression is used as a ground processor pre-flight to develop a statistical classifier. The classifier would then be implemented in flight software and executed in real-time. This technique offers improved performance even in the face of highly inaccurate measurements. Although the logistic regression-based trigger approach will not be implemented within EFT-1 flight software, the methodology can be carried forward for future missions and vehicles.

Smith, Kelly M.↗

NASA NTRS2013

An Alternative Flight Software Paradigm: Applying Multivariate Logistic Regression to Sense Trigger Conditions using Inaccurate or Scarce Information

Smith, Kelly↗

NASA NTRS2011

Automated Miniaturized Instrument for Space Biology Applications and the Monitoring of the Astronauts Health Onboard the ISS

Human space travelers experience a unique environment that affects homeostasis and physiologic adaptation. The spacecraft environment subjects the traveler to noise, chemical and microbiological contaminants, increased radiation, and variable gravity forces. As humans prepare for long-duration missions to the International Space Station (ISS) and beyond, effective measures must be developed, verified and implemented to ensure mission success. Limited biomedical quantitative capabilities are currently available onboard the ISS. Therefore, the development of versatile instruments to perform space biological analysis and to monitor astronauts' health is needed. We are developing a fully automated, miniaturized system for measuring gene expression on small spacecraft in order to better understand the influence of the space environment on biological systems. This low-cost, low-power, multi-purpose instrument represents a major scientific and technological advancement by providing data on cellular metabolism and regulation. The current system will support growth of microorganisms, extract and purify the RNA, hybridize it to the array, read the expression levels of a large number of genes by microarray analysis, and transmit the measurements to Earth. The system will help discover how bacteria develop resistance to antibiotics and how pathogenic bacteria sometimes increase their virulence in space, facilitating the development of adequate countermeasures to decrease risks associated with human spaceflight. The current stand-alone technology could be used as an integrated platform onboard the ISS to perform similar genetic analyses on any biological systems from the tree of life. Additionally, with some modification the system could be implemented to perform real-time in-situ microbial monitoring of the ISS environment (air, surface and water samples) and the astronaut's microbiome using 16SrRNA microarray technology. Furthermore, the current system can be enhanced substantially by combining it with other technologies for automated, miniaturized, high-throughput biological measurements, such as fast sequencing, protein identification (proteomics) and metabolite profiling (metabolomics). Thus, the system can be integrated with other biomedical instruments in order to support and enhance telemedicine capability onboard ISS. NASA's mission includes sustained investment in critical research leading to effective countermeasures to minimize the risks associated with human spaceflight, and the use of appropriate technology to sustain space exploration at reasonable cost. Our integrated microarray technology is expected to fulfill these two critical requirements and to enable the scientific community to better understand and monitor the effects of the space environment on microorganisms and on the astronaut, in the process leveraging current capabilities and overcoming present limitations.

Human space travelers↗

DOE OSTI2020

Time-sequence Spectroscopy of Epsilon CrA: The 518 nm Mg i Triplet Region Analyzed with Broadening Functions

High-resolution spectroscopic observations of the W UMa-type binary ϵ CrA obtained as a time-monitoring sequence on four full and four partial nights within two weeks have been used to derive orbital elements of the system and discuss the validity of the Lucy model for description of the radial-velocity data. The observations had more extensive temporal coverage and better quality than similar time-sequence observations of the contact binary AW UMa. The two binaries share several physical properties and show very similar deviations from the Lucy model: the primary component is a rapidly rotating star almost unaffected by the presence of the secondary component, while the latter is embedded in a complex gas flow and appears to have its own rotation-velocity field, in contradiction to the model. The spectroscopic mass ratio is found to be larger than the one derived from the light-curve analysis, as in many other W UMa-type binaries, but the discrepancy for ϵ CrA is relatively minor, much smaller than for AW UMa. The presence of the complex velocity flows contradicting the assumption of solid-body rotation suggests a necessity of modification to the Lucy model, possibly along the lines outlined by Stȩpień in his concept of energy transfer between the binary components.

79 ASTRONOMY AND ASTROPHYSICS↗

NASA NTRS1979

Ultraviolet spectrum of the Aurora /2000-2800 A/

Ultraviolet spectra between 2000 and 2800 delta-v of an IBC II(+) aurora were obtained by a rocket-borne Ebert-Fastie monochromator at 15-A resolution over Fort Churchill, Manitoba. The v-prime = 0-8 progressions of the N2 Vegard-Kaplan system, the delta-v = 8 sequence of the Lyman-Birge-Hopfield system, and a progression attributed to the Herman-Kaplan system were identified by a synthetic spectrum analysis. Relative populations of the first eight vibrational levels of the A3 Sigma u(+) state at high altitude were obtained from the theoretically generated spectra, and quenching rate coefficients were derived for these eight levels by using an atomic oxygen concentration derived from data from another experiment on board. For v = 0 the quenching rate coefficient was found to be 2.0 times 10 the minus 10th cu cm/sec and was not found to increase for higher vibrational levels. The intensity of the (0,2) band of the Herman-Kaplan system was found to be about 5 times the theoretically predicted value, but even so, the cascade from E3 Sigma g(+) provides less than 2% of the A3 Sigma u(+) state population.

Beiting, E. J., III↗

NASA NTRS2003

Microbial Diversity Aboard Spacecraft: Evaluation of the International Space Station

An evaluation of the microbial flora from air, water, and surface samples provided a baseline of microbial diversity onboard the International Space Station (ISS) to gain insight into bacterial and fungal contamination during the initial stages of construction and habitation. Using 16S genetic sequencing and rep-PeR, 63 bacterial strains were isolated for identification and fingerprinted for microbial tracking. The use of these molecular tools allowed for the identification of bacteria not previously identified using automated biochemical analysis and provided a clear indication of the source of several ISS contaminants. Fungal and bacterial data acquired during monitoring do not suggest there is a current microbial hazard to the spacecraft, nor does any trend indicate a potential health risk. Previous spacecraft environmental analysis indicated that microbial contamination will increase with time and require continued surveillance.

Castro, Victoria A.↗

NASA NTRS2017

Performance Enhancement of a Computational Persistent Homology Package

In recent years, persistent homology has become an attractive method for data analysis. It captures topological features, such as connected components, holes, voids, etc., from a point cloud by finding out when these features appear and disappear in the filtration sequence. In this project, we focus on improving the performance of Eirene, a fancy computational persistent homology package. Eirene is a 5000-line opensource software implemented by using the dynamic programming language Julia. We use the Julia profiling tools to identify the performance bottlenecks and develop different methods to manage the bottlenecks, including the parallelization of some time-consuming functions on the multicore/manycore hardware. The empirical results show that the performance can be greatly improved.

Profiling↗

DOE OSTI2021

Preliminary Analysis of Source Physics Experiment Explosion-Triggered Microseismicity Using the Back-Projection Method

A series of four chemical explosions were detonated in a deep borehole within the Yucca Flat Dry Alluvium Geology (DAG) at the Nevada National Security Site between 2018 and 2019. The two larger chemical explosions of 50 tons (DAG-2) and 10 tons (DAG-4) TNT equivalent yield triggered energetic aftershock sequences numbering 1392 and 347 microearthquakes, respectively, within the first 10 days. No significant aftershock activity was observed for the two smaller 1-ton explosions (DAG-1 and DAG-3). Here, we used a back-projection method based on travel-time migration and stacking of signal-to-noise ratio traces to detect, associate and locate aftershocks from a subset of 22-geophones within a larger 2 urn:x-wiley:21699313:media:jgrb54910:jgrb54910-math-0001 2 km seismic array surrounding the borehole. The aftershocks located within 300 m of the borehole and the depths were above the working points of 300 and 50 m depths of DAG-2 and DAG-4, respectively, ruling out triggering slip on geologic faults or disturbances beneath neighboring collapse craters. DAG-2 and DAG-4 aftershocks decayed at similar rates, with power-law exponents of p = 1.48 and p = 1.49, respectively. These decay rates are comparable to aftershocks sequences triggered by earthquakes and historical nuclear explosions at Yucca Flat. A smooth power-law aftershock decay within the first 10 days suggests a triggering mechanism from explosion generated stress relaxation due to the diffusion of high gas pressures in the cavity and radial fractures. A more random and episodic aftershock rate would be expected due to cavity collapse or falling rubble in chimney formation.

58 GEOSCIENCES↗

NASA NTRS2019

Does Seed Sanitization Affect the Plant Rhizosphere Microbiome and Its Ability to Compete with the Human Associated Pathogen, E. coli on Salad Crops?

Cultivation of crops in controlled environmental agricultural systems may limit microbial colonization and reduce diversity of the microbial communities. Practices like seed and growth medium sanitization may further impact microbial communities in the mature plant and the plant’s capacity to limit the growth of pathogens through competition. As humans expand their travels to space, understanding plant growth, health, and development in closed environments will be critical to the success of producing a safe, supplemental food source for astronauts. To determine the persistence of a potential human pathogen in plant growth and development, sanitized and unsanitized seeds from, mizuna (Brassica rapa var japonica) and red romaine lettuce (Lactuca sativa cultivar ‘Outredgeous’), were inoculated with Escherichia coli, ATCC 21445, germinated under simulated International Space Station (ISS) environmental conditions and harvested every 7 days until maturity. The persistence of E. coli in the rhizosphere was determined by plating on selective media, real time PCR (Polymerase Chain Reaction) and community sequencing of the rhizosphere communities. E. coli was detected in the crops’ roots and leaves for several weeks post germination. At day 28, plants from sanitized seeds had significantly higher counts of E. coli on the roots than those from unsanitized seeds. E. coli was also detected on a few uninoculated plants indicating airborne cross contamination among plants in the same growth chamber and suggesting an influence of the natural microbiome on human pathogen survival and persistence in leafy greens. Sequencing analysis revealed variations in composition and diversity between the communities. Understanding the microbial community of the rhizospheric microbiome is only the first step in determining the relationships between plants. Additional studies to include genotypic and phenotypic variations in the plants should be considered to determine if the natural microbes in the rhizosphere may contribute to the health and therefore, safety of the edible plants.

Khodadad, Christina L. M.↗

NASA NTRS2019

Tuning the Performance of a Computational Persistent Homology Package

In recent years, persistent homology has become an attractive method for data analysis. It captures topological features, such as connected components, holes, and voids from point cloud data and summarizes the way in which these features appear and disappear in a filtration sequence. In this project, we focus on improving the performanceof Eirene, a computational package for persistent homology. Eirene is a 5000-line open-source software library implemented in the dynamic programming language Julia. We use the Julia profiling tools to identify performance bottlenecks and develop novel methods to manage them, including the parallelization of some time-consuming functions on multicore/manycore hardware. Empirical results show that performance can be greatly improved.

Persistent Homology↗

DOE OSTI2022

Conserved conformational dynamics determine enzyme activity

Homologous enzymes often exhibit different catalytic rates despite a fully conserved active site. The canonical view is that an enzyme sequence defines its structure and function and, more recently, that intrinsic protein dynamics at different time scales enable and/or promote catalytic activity. Here, we show that, using the protein tyrosine phosphatase PTP1B, residues surrounding the PTP1B active site promote dynamically coordinated chemistry necessary for PTP1B function. However, residues distant to the active site also undergo distinct intermediate time scale dynamics and these dynamics are correlated with its catalytic activity and thus allow for different catalytic rates in this enzyme family. We identify these previously undetected motions using coevolutionary coupling analysis and nuclear magnetic resonance spectroscopy. Our findings strongly indicate that conserved dynamics drives the enzymatic activity of the PTP family. Characterization of these conserved dynamics allows for the identification of novel regulatory elements (therapeutic binding pockets) that can be leveraged for the control of enzymes.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2022

Long-Term and Low-Level Envelope C2V3 Stimulation by Highly Diverse Virus Isolates Leads to Frequent Development of Broad and Elite Antibody Neutralization in HIV-1-Infected Individuals

A minority of HIV-1-infected patients produce broadly neutralizing antibodies (bNAbs). Identification of viral and host correlates of bNAb production may help develop vaccines. We aimed to characterize the neutralizing response and viral and host-associated factors in Angola, which has one of the oldest, most dynamic, and most diverse HIV-1 epidemics in the world. Three hundred twenty-two HIV-1-infected adults from Angola were included in this retrospective study. Phylogenetic analysis of C2V3C3 env gene sequences was used for virus subtyping. Env-binding antibody reactivity was tested against polypeptides comprising the C2, V3, and C3 regions. Neutralizing-antibody responses were determined against a reference panel of tier 2 Env pseudoviruses in TZM-bl cells; neutralizing epitope specificities were predicted using ClustVis. All subtypes were found, along with untypeable strains and recombinant forms. Notably, 56% of the patients developed cross neutralizing, broadly neutralizing, or elite neutralizing responses. Broad and elite neutralization was associated with longer infection time, subtype C, lower CD4 + T cell counts, higher age, and higher titer of C2V3C3-specific antibodies relative to failure to develop bNAbs. Neutralizing antibodies targeted the V3-glycan supersite in most patients. V3 and C3 regions were significantly less variable in elite neutralizers than in weak neutralizers and nonneutralizers, suggesting an active role of V3C3-directed bNAbs in controlling HIV-1 replication and diversification. In conclusion, prolonged and low-level envelope V3C3 stimulation by highly diverse and ancestral HIV-1 isolates promotes the frequent elicitation of bNAbs. These results provide important clues for the development of an effective HIV-1 vaccine. Studies on neutralization by antibodies and their determinants in HIV-1-infected individuals have mostly been conducted in relatively recent epidemics caused by subtype B and C viruses. Results have suggested that elicitation of broadly neutralizing antibodies (bNAbs) is uncommon. The mechanisms underlying the elicitation of bNAbs are still largely unknown. We performed the first characterization of the plasma neutralizing response in a cohort of HIV-1-infected patients from Angola. Angola is characterized by an old and dynamic epidemic caused by highly diverse HIV-1 variants. Remarkably, more than half of the patients produced bNAbs, mostly targeting the V3-glycan supersite in HIV-1. This was associated with higher age, longer infection time, lower CD4 + T cell counts, subtype C infection, or higher titer of C2V3C3-specific antibodies relative to patients that did not develop bNAbs. These results may help develop the next generation of vaccine candidates for HIV-1.

59 BASIC BIOLOGICAL SCIENCES↗

NASA NTRS1978

Relative timing of rifting and volcanism on earth and its tectonic implications

It is suggested that two basic types of rifting-volcanism relative timing might be related to two basic modes of rifting. In one sequence, volcanism and, usually, local doming predate major rift formation, while in the other type, rifts form first and volcanism follows. In the first mode of rifting, the mantle plays an active role and convection 'plumes' dome up and crack the lithosphere, while in the second case, the horizontal movements of plates give rise to extension of the lithosphere and induce rifting, and the mantle is passive. Since numerous local conditions complicate this simple pattern, detailed stratigraphic/structural analysis of individual rifts is recommended.

Sengor, A. M. C.↗

NASA NTRS1991

Space architecture monograph series. Volume 4: Genesis 2: Advanced lunar outpost

This research and design study investigated advanced lunar habitats for astronauts and mission specialists on the Earth's moon. Design recommendations are based on environmental response to the lunar environment, human habitability (human factors and environmental behavior research), transportability (structural and materials system with least mass), constructability (minimizing extravehicular time), construction dependability and resilience, and suitability for NASA launch research missions in the 21st century. The recommended design uses lunar lava tubes, with construction being a combination of Space Station Freedom derived hard modules and light weight Kevlar laminate inflatable structures. The proposed habitat includes research labs and a biotron, crew quarters and crew support facility, mission control, health maintenance facility, maintenance work areas for psychological retreat, privacy, and comtemplation. Furniture, specialized equipment, and lighting are included in the analysis and design. Drawings include base master plans, construction sequencing, overall architectural configuration, detailed floor plans, sections and axonometrics, with interior perspectives.

Fieber, Joseph P.↗