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At least 541 records · Page 30

TRACER-Uncrewed Aircraft System (TRACER-UAS) Field Campaign Report

Convective processes drive the development of clouds across a variety of scales, thereby playing a central role in governing weather and climate globally. These processes help to set the stage for circulations that redistribute heat and moisture throughout the lower atmosphere, and simultaneously develop storms that supply precipitation to regions that depend on such rain events for supporting a variety of societal needs. At the same time, extreme precipitation events can result in localized flooding of low-lying areas, posing hazards to transportation and regional inhabitants, and potentially damaging structures and infrastructure. As a result, there is an inherent need to better understand the drivers and modulators of clouds and precipitation and leverage such understanding to make projections of how these important systems may evolve with a changing climate. With nearly three billion of Earth’s human inhabitants living within 200 km of a shoreline, development of such understanding is particularly important in coastal regimes. Central drivers of convective cloud development and life cycle in coastal environments include synoptic forcing, local meso- and microscale circulation regimes, microphysical evolution, and aerosol properties. Atmospheric boundary layer (ABL) development and its evolution can help drive the formation and organization of convective clouds, which then impart their own controls through dynamic processes related to latent heating and cooling (including cold-pool formation and propagation), three-dimensional radiative effects, and background environmental conditions (Fan et al. 2016). Such clouds have been contributing to record precipitation events in southeast Texas in recent years, relative to the last several decades of data from regional precipitation gauges (Fagnant et al. 2020). The greater Houston urban area has experienced particularly large increases in the frequency of extreme rainfall events, with enhanced intensity of extreme events (stretching of distribution tails) having potentially significant implications for watershed floodplain planning and mapping in greater Houston and Harris County. While the most extreme precipitation events were associated with tropical cyclones (e.g., Tropical Storm Allison and Hurricane Harvey; Blood 2014, Kao et al. 2019), strong thunderstorms can also deliver large amounts of precipitation over the area over relatively short periods.

54 ENVIRONMENTAL SCIENCES↗

Discovering the Multisectoral Impacts of Global Energy Sector Outcomes Through Multiple Ensemble Aggregation Measures

Understanding complex human-Earth system interactions often involves analyzing large scenario ensembles that encompass a wide range of plausible futures. These ensembles often require aggregation to summarize information based on specific criteria or conditions. However, previous research using global change scenario ensembles has largely overlooked how the choice of aggregation method influences the interpretation of results. To address this gap, we leverage a large ensemble data set designed to capture broad energy system dynamics generated using the Global Change Analysis Model. We first explore how energy-related uncertainties are propagated to both global and regional water-energy-food sectors. We then conduct a rank correlation analysis across seven ensemble aggregation measures and demonstrate the need to consider multiple measures in global change scenarios. Our results suggest that global water and food sector outcomes in the 21st century vary widely depending on different scenario assumptions. The global energy productivity is projected to improve by the end of the century across all scenarios. Moreover, regions facing water scarcity challenges in 2100 do not always overlap with those facing extreme energy and food sector outcomes. Although rank correlations across seven aggregation measures are relatively stable across sectors, we identify cases where relying on a single measure leads to losing critical information in the full ensemble. Reliance on a single aggregation measure can distort the interpretation of global change scenario outcomes. Instead, adopting multiple ensemble aggregation measures provides a more holistic understanding of global change scenario ensembles.

Kim, Gijoo↗

Antiviral discovery using sparse datasets by integrating experiments, molecular simulations, and machine learning

Computational methods have demonstrated success in identifying virucidal agents, effectively contributing to the discovery of novel virucidal molecules. In this study, we developed a machine learning (ML) model, trained on a small dataset, to predict inhibitors of human enterovirus 71 (EV71), a pathological agent that causes severe disease in children and immunocompromised adults. Despite the dataset’s limitation, comprising of only 36 compounds tested, our ML framework demonstrated significant predictive capability. Notably, experimental validation revealed that five out of the eight compounds predicted by our model from the Chinese cosmetic material list exhibited virucidal activity. The inhibitor effects displayed by the main active compounds were further confirmed by molecular dynamics simulation. This underscores the potential of our AI-driven approach to bypass data constraints in identifying active molecules against viral pathogens.

60 APPLIED LIFE SCIENCES↗

Global urban growth between 1870 and 2100 from integrated high resolution mapped data and urban dynamic modeling

Long term, global records of urban extent can help evaluate environmental impacts of anthropogenic activities. Remotely sensed observations can provide insights into historical urban dynamics, but only during the satellite era. Here, we develop a 1 km resolution global dataset of annual urban dynamics between 1870 and 2100 using an urban cellular automata model trained on satellite observations of urban extent between 1992 and 2013. Hindcast (1870–1990) and projected (2020–2100) urban dynamics under the five Shared Socioeconomic Pathways (SSPs) were modeled. We find that global urban growth under SSP5, the fossil-fuelled development scenario, was largest with a greater than 40-fold increase in urban extent since 1870. The high resolution dataset captures grid level urban sprawl over 200 years, which can provide insights into the urbanization life cycle of cities and help assess long-term environmental impacts of urbanization and human–environment interactions at a global scale.

54 ENVIRONMENTAL SCIENCES↗

Integrating Viral Metagenomics into an Ecological Framework

Viral metagenomics has expanded our knowledge of the ecology of uncultured viruses, within both environmental (e.g., terrestrial and aquatic) and host-associated (e.g., plants and animals, including humans) contexts. Here, we emphasize the implementation of an ecological framework in viral metagenomic studies to address questions in virology rarely considered ecological, which can change our perception of viruses and how they interact with their surroundings. Additionally, an ecological framework explicitly considers diverse variants of viruses in populations that make up communities of interacting viruses, with ecosystem-level effects. It provides a structure for the study of the diversity, distributions, dynamics, and interactions of viruses with one another, hosts, and the ecosystem, including interactions with abiotic factors. An ecological framework in viral metagenomics stands poised to broadly expand our knowledge in basic and applied virology. We highlight specific fundamental research needs to capitalize on its potential and advance the field.

59 BASIC BIOLOGICAL SCIENCES↗

Integrative structural and functional analysis of human malic enzyme 3: A potential therapeutic target for pancreatic cancer

Malic enzymes (ME1, ME2, and ME3) are involved in cellular energy regulation, redox homeostasis, and biosynthetic processes, through the production of pyruvate and reducing agent NAD(P)H. Recent studies have implicated the third and least well-characterized isoform, mitochondrial NADP + -dependent malic enzyme 3 (ME3), as a therapeutic target for pancreatic cancers. Here, we utilized an integrated structure approach to determine the structures of ME3 in various ligand-binding states at near-atomic resolutions. ME3 is captured in the open form existing as a stable tetramer and its dynamic Domain C is critical for activity. Catalytic assay results reveal that ME3 is a non-allosteric enzyme and does not require modulators for activity while structural analysis suggests that the inner stability of ME3 Domain A relative to ME2 disables allostery in ME3. With structural information available for all three malic enzymes, the foundation has been laid to understand the structural and biochemical differences of these enzymes and could aid in the development of specific malic enzyme small molecule drugs.

60 APPLIED LIFE SCIENCES↗

Ecohydrological decoupling under changing disturbances and climate

Terrestrial disturbances are increasing in frequency and severity, perturbing the hydrologic cycle by altering vegetation-mediated water use and microclimate. Changes in the hydrological cycle alter vegetation succession, which is additionally influenced by changing climate and atmospheric CO 2 . Here, the interacting drivers and feedbacks between disturbances, climate, and vegetation cause uncertainty regarding the sustainable provision of freshwater through streamflow. Here we synthesize the literature on post-disturbance ecohydrological coupling, in particular, including the mechanistic relationship between vegetation and streamflow, under changing disturbance regimes, atmospheric CO 2 , and climate. Disturbance can cause decoupling between transpiration and streamflow by altering the connectivity, size, availability, and spatial distribution of their source pools. Decoupling increases when soil moisture is low due to reduced connectivity, and when soil moisture is high and Q and T are independent. Successional trajectories regulate the physiological and physical features influencing influence the dynamics of source water partitioning. Changing climate and disturbance regimes can alter succession and prolong decoupling, with potential moderation by CO 2 . Increasing rates, severity, and spread of disturbances along with warming could promote greater decoupling globally. From this review emerges a framework of testable hypotheses that identify the critical processes regulating ecohydrological coupling and provides a roadmap for future research. Accurate prediction of post-disturbance coupling and subsequent water availability for human consumption requires understanding the degree of hydraulic connectivity between source water pools for transpiration and streamflow and their response to succession under changing disturbance and climate regimes.

54 ENVIRONMENTAL SCIENCES↗

Active site remodeling in tumor-relevant IDH1 mutants drives distinct kinetic features and potential resistance mechanisms

Abstract Mutations in human isocitrate dehydrogenase 1 (IDH1) drive tumor formation in a variety of cancers by replacing its conventional activity with a neomorphic activity that generates an oncometabolite. Little is understood of the mechanistic differences among tumor-driving IDH1 mutants. We previously reported that the R132Q mutant unusually preserves conventional activity while catalyzing robust oncometabolite production, allowing an opportunity to compare these reaction mechanisms within a single active site. Here, we employ static and dynamic structural methods and observe that, compared to R132H, the R132Q active site adopts a conformation primed for catalysis with optimized substrate binding and hydride transfer to drive improved conventional and neomorphic activity over R132H. This active site remodeling reveals a possible mechanism of resistance to selective mutant IDH1 therapeutic inhibitors. This work enhances our understanding of fundamental IDH1 mechanisms while pinpointing regions for improving inhibitor selectivity.

60 APPLIED LIFE SCIENCES↗

Resilient Inverter-Driven Black Start with Collective Parallel Grid-Forming Operation

As modern power systems are experiencing exceptional changes with increasing penetrations of inverter-based resources (IBRs), system restoration using IBRs has received attention. Using local grid-forming (GFM) assets near consumers, engineered to establish grid voltages in the absence of a stiff grid, i.e., bottom-up restoration, a distribution system could obtain high system resilience by not relying on the bulk power system restoration, which requires significant human intervention and procedure. This paper studies the technical feasibility of the novel approach with detailed electromagnetic transient (EMT) simulations. To thoroughly evaluate the potential of GFM inverters and the technical challenges in IBR-driven black start, a detailed three-phase inverter model is developed, including negative-sequence control for voltage balance and a phase-by- phase current limiter to sustain momentary overloading during the black start. To examine dynamic aspects of the black-start process, the EMT simulation also models transformer and motor dynamics to emulate their inrush and startup behaviors as well as network dynamics. In addition, active involvement of grid- following distributed energy resources is also studied to facilitate the black-start process. By allowing multiple GFM inverters to collectively black start without leader-follower coordination, we demonstrate that a system can achieve high resilience even with a fraction of assets lost. Two test cases of inverter-driven black start, using two and one GFM inverters, respectively, for a heavily unbalanced 2-MVA distribution feeder are demonstrated. Takeaways for further study and field deployment are provided.

grid-forming inverter↗

A computational modeling framework for pre-clinical evaluation of cardiac mapping systems

There are a variety of difficulties in evaluating clinical cardiac mapping systems, most notably the inability to record the transmembrane potential throughout the entire heart during patient procedures which prevents the comparison to a relevant “gold standard”. Cardiac mapping systems are comprised of hardware and software elements including sophisticated mathematical algorithms, both of which continue to undergo rapid innovation. The purpose of this study is to develop a computational modeling framework to evaluate the performance of cardiac mapping systems. The framework enables rigorous evaluation of a mapping system’s ability to localize and characterize (i.e., focal or reentrant) arrhythmogenic sources in the heart. The main component of our tool is a library of computer simulations of various dynamic patterns throughout the entire heart in which the type and location of the arrhythmogenic sources are known. Our framework allows for performance evaluation for various electrode configurations, heart geometries, arrhythmias, and electrogram noise levels and involves blind comparison of mapping systems against a “silver standard” comprised of computer simulations in which the precise transmembrane potential patterns throughout the heart are known. A feasibility study was performed using simulations of patterns in the human left atria and three hypothetical virtual catheter electrode arrays. Activation times (AcT) and patterns (AcP) were computed for three virtual electrode arrays: two basket arrays with good and poor contact and one high-resolution grid with uniform spacing. The average root mean squared difference of AcTs of electrograms and those of the nearest endocardial action potential was less than 1 ms and therefore appears to be a poor performance metric. In an effort to standardize performance evaluation of mapping systems a novel performance metric is introduced based on the number of AcPs identified correctly and those considered spurious as well as misclassifications of arrhythmia type; spatial and temporal localization accuracy of correctly identified patterns was also quantified. This approach provides a rigorous quantitative analysis of cardiac mapping system performance. Proof of concept of this computational evaluation framework suggests that it could help safeguard that mapping systems perform as expected as well as provide estimates of system accuracy.

59 BASIC BIOLOGICAL SCIENCES↗

ML based control systems for nuclear physics experiments

The Experimental Physics Software and Computing Infrastructure (EPSCI) group at Jefferson Lab is leading the use of machine learning (ML) to enhance control systems in nuclear physics experiments. Collaborating closely with domain experts and data scientists, we have developed an ML-based control system that uses a Gaussian process to dynamically adjust the high voltage of the GlueX Central Drift Chamber. This results in stable detector performance by adapting to environmental changes, thereby reducing the offline calibration effort. Furthermore, we are developing ML-driven systems for optimizing the polarization of photon beams and polarized cryotargets. These systems will maintain the optimal microwave frequency in cryogenic targets and make real-time adjustments to diamond radiators for polarized photon sources, tasks traditionally handled by human operators. By automating these functions, we aim to optimize the polarization, reduce downtime, and minimize human error. This talk will highlight the development of reliable ML-based control systems and the policies to ensure they are both effective and trustworthy.

Jeske, Torri↗

Resilient Inverter-Driven Black Start with Collective Parallel Grid-Forming Operation: Preprint

As the modern power systems are experiencing exceptional changes with increasing penetration of inverter-based resources (IBRs), system restoration using IBRs has received attention. Using local grid-forming (GFM) assets near consumers, engineered to establish grid voltages in the absence of a stiff grid, i.e., bottom-up restoration, a distribution system would obtain high system resilience, by not relying on the bulk power system restoration requiring significant human intervention and procedure to restore. This paper studies the technical feasibility of the novel approach with detailed electromagnetic transient (EMT) simulations. To thoroughly evaluate the potential of GFM inverters and technical challenges in IBR-driven black start, a detailed three-phase inverter model is developed, including negative-sequence control for voltage balance and a phase-by-phase current limiter to sustain momentary overloading during the black start. To examine dynamic aspects of the black start process, the EMT simulation also models transformer and motor dynamics to emulate their inrush and start-up behaviors as well as network dynamics. In addition, active involvement of grid-following distributed energy resources (DER) is also studied to facilitate the black start process. It is shown that, by allowing multiple GFM inverters to collectively black start without master-slave coordination, a system can achieve high resilience even with a fraction of assets lost. Two test cases of inverter-driven black start using two and one GFM inverters, respectively, for a heavily unbalanced 2-MVA distribution feeder are demonstrated. Takeaways for further study and field deployment are provided.

grid-forming inverter↗

‪A Novel Methodology for Longitudinal Studies of Home Thermal Comfort Perception and Behavior

Human-building interactions significantly influence building energy consumption and affect peak energy demand. For example, heating and cooling contribute 46% of daily peak residential energy demand. Grid-interactive efficient buildings (GEBs) can potentially increase energy-demand flexibility and accelerate the adoption of renewables. However, traditional demand response (DR) programs focused on shedding peak loads disregard the human-building interactions leading to occupant thermal frustration. Specifically, they do not model occupants’ ability to override thermostat controls, nor how indoor environmental conditions and sociocultural factors affect the timing and magnitude of overrides. Studies have found 20% of occupants override thermostat setpoints during DR events longer than 6 hours yet lack detail about the motivation that might guide more successful efforts. Balancing energy-demand flexibility with occupant thermal comfort requires understanding dynamic occupant behavior, the underlying psychophysiological drivers, in the context of homes. This paper presents methods for scalable longitudinal studies of residential occupant behavior dynamics to inform the development of psychophysiological occupant-centric building models. Smart sensors were installed to measure local environmental conditions in 20 homes in two regions of the United States. Just-in-time ecological momentary assessments (EMAs) provided qualitative data on occupant thermal comfort and local environmental conditions not captured in Internet-of-Things (IoT) based studies or existing datasets. Participant interviews provided insight into environmental attitudes, mental models, and the role of economics in comfort and behavior, which in turn may affect thermostat interactions. Based on these data, interventions in the next phase of the study will collect data and monitor occupant behavior during simulated DR events.

Kane, Michael↗

Broadly cross-reactive human antibodies that inhibit genogroup I and II noroviruses

The rational development of norovirus vaccine candidates requires a deep understanding of the antigenic diversity and mechanisms of neutralization of the virus. Here, we isolate and characterize a panel of broadly cross-reactive naturally occurring human monoclonal IgMs, IgAs and IgGs reactive with human norovirus (HuNoV) genogroup I or II (GI or GII). We note three binding patterns and identify monoclonal antibodies (mAbs) that neutralize at least one GI or GII HuNoV strain when using a histo-blood group antigen (HBGA) blocking assay. The HBGA blocking assay and a virus neutralization assay using human intestinal enteroids reveal that the GII-specific mAb NORO-320, mediates HBGA blocking and neutralization of multiple GII genotypes. The Fab form of NORO-320 neutralizes GII.4 infection more potently than the mAb, however, does not block HBGA binding. The crystal structure of NORO-320 Fab in complex with GII.4 P-domain shows that the antibody recognizes a highly conserved region in the P-domain distant from the HBGA binding site. Dynamic light scattering analysis of GII.4 virus-like particles with mAb NORO-320 shows severe aggregation, suggesting neutralization is by steric hindrance caused by multivalent cross-linking. Aggregation was not observed with the Fab form of NORO-320, suggesting that this clone also has additional inhibitory features.

59 BASIC BIOLOGICAL SCIENCES↗

Towards modeling phage therapy

Patients infected with life-threatening multi-drug resistant (MDR) bacteria have been treated with cocktails of bacteriophages. This is a complicated form of personalized medicine as the phages given to a patient have to be selected beforehand on the basis of their lytic capacity of the infecting bacteria. Because bacteria rapidly become resistant, the evolution of resistance to a diverse cocktail of phages is a complicated dynamical process, during which competing bacterial strains replace one another by accumulating several resistance mechanisms, each of which may involve a fitness cost. As a consequence, it is typically not known why a particular phage therapy succeeded or failed, and how one can optimize the composition of the cocktails to maximize the rate of success. To improve upon this, we extend an existing in vivo -calibrated mouse model into a novel mathematical model for the human situation, and include multiple phages infecting multiple bacterial strains, differing in their resistance to each of the phages. We adjust several parameter estimates of the bacterial model to the human situation, and use the model to describe a successful case of phage therapy involving several cocktails, each containing several phages. In the model, treatment success crucially depended on pretreatment resistance levels, and on the diversity and the timing of the cocktails. Once an appropriate cocktail is found, it is less important to further optimize the infection rates of the phages. Resistant bacterial strains expand rapidly when sensitive strains decline, and the higher the infectivity of the phages, the faster resistant strains expand. Because resistance evolves rapidly, it is best to provide a diverse set of phages right from the start of therapy, i.e., to hit hard and early, and create a high genetic barrier to bacterial resistance.

59 BASIC BIOLOGICAL SCIENCES↗

The Development of the Pooled Rideshare Acceptance Model (PRAM)

Due to the advancements in real-time information communication technologies and sharing economies, rideshare services have gained significant momentum by offering dynamic and/or on-demand services. Rideshare service companies evolved from personal rideshare, where riders traveled solo or with known individuals, into pooled rideshare (PR), where riders can travel with one to multiple unknown riders. Similar to other shared economy services, pooled rideshare is beneficial as it efficiently utilizes resources, resulting in reduced energy usage, as well as reduced costs for the riders. However, previous research has demonstrated that riders have concerns about using pooled rideshare, especially regarding personal safety. A U.S. national survey with 5385 participants was used to understand human factor-related barriers and user preferences to develop a novel Pooled Rideshare Acceptance Model (PRAM). This model used a covariance-based structural equation model (CB-SEM) to identify the relationships between willingness to consider PR factors (time/cost, privacy, safety, service experience, and traffic/environment) and optimizing one’s experience of PR factors (vehicle technology/accessibility, convenience, comfort/ease of use, and passenger safety), resulting in the higher-order factor trust service. We examined the factors’ relative contribution to one’s willingness/attitude towards PR and user acceptance of PR. Privacy, safety, trust service, and convenience were statistically significant factors in the model, as were the comfort/ease of use factor and the service experience, traffic/environment, and passenger safety factors. The only two non-significant factors in the model were time/cost and vehicle technology/accessibility; it is only when a rider feels safe that individuals then consider the additional non-significant variables of time, cost, technology, and accessibility. Privacy, safety, and service experience were factors that discouraged the use of PR, whereas the convenience factor greatly encouraged the acceptance of PR. Despite the time/cost factor’s lack of significance, individual items related to time and cost were crucial when viewed within the context of convenience. This highlights that while user perceptions of privacy and safety are paramount to their attitude towards PR, once safety concerns are addressed, and services are deemed convenient, time and cost elements significantly enhance their trust in pooled rideshare services. This study provides a comprehensive understanding of user acceptance of PR services and offers actionable insights for policymakers and rideshare companies to improve their services and increase user adoption.

dynamic rideshare system↗

Light Water Sustainability Program: Optimizing Information Automation Using a New Method Based on System-Theoretic Process Analysis

This report describes the interim progress for research supporting the design and optimization of information automation systems for nuclear power plants. Much of the domestic nuclear fleet is currently focused on modernizing technologies and processes, including transitioning toward digitalization in the control room and elsewhere throughout the plant, along with a greater use of automation, artificial intelligence, robotics, and other emerging technologies. While there are significant opportunities to apply these technologies toward greater plant safety, efficiency, and overall cost-effectiveness, optimizing their design and avoiding potential safety and performance risks depends on ensuring that human-performance-related organizational and technical design issues are identified and addressed. This report describes modeling tools and techniques, based on sociotechnical system theory, to support these design goals and their application in the current research effort. The report is intended for senior nuclear energy stakeholders, including regulators, corporate management, and senior plant management. We have developed and employed a method to design an optimized information automation ecosystem (IAE) based on the systems-theoretic constructs underlying sociotechnical systems theory in general and the Systems-Theoretic Accident Modeling and Processes (STAMP) approach in particular. We argue that an IAE can be modeled as an interactive information control system whose behavior can be understood in terms of dynamic control and feedback relationships amongst the system’s technical and organizational components. Up to this point, we have employed a Causal Analysis based on STAMP (CAST) technique to examine a performance- and safety-related incident at an industry partner’s plant that involved the unintentional activation of an emergency diesel generator. This analysis provided insight into the behavior of the plant’s current information control structure within the context of a specific, significant event. Our ongoing analysis is focused on identifying near-term process improvements and longer-term design requirements for an optimized IAE system. The latter analyses will employ a second STAMP-derived technique, System-Theoretic Process Analysis (STPA). STPA is a useful modeling tool for generating and analyzing actual or potential information control structures. Finally, we have begun modeling plantwide organizational relationships and processes. Organizational system modeling will supplement our CAST and STPA findings and provide a basis for mapping out a plantwide information control architecture. CAST analysis findings indicate an important underlying contributor to the incident under investigation, and a significant risk to information automation system performance, was perceived schedule pressure, which exposed weaknesses in interdepartmental coordination between and within responsible plant organizations and challenged the resilience of established plant processes, until a human caused the initiating event. These findings are discussed in terms of their risk to overall system performance and their implications for information automation system resilience and brittleness. We present two preliminary information automation models. The proactive issue resolution model is a test case of an information automation concept with significant near-term potential for application and subsequent reduction in significant plant events. The IAE model is a more general representation of a broader, plantwide information automation system. From our results, we have generated a set of preliminary system-level requirements and safety constraints. These requirements will be further developed over the remainder of our project in collaboration with nuclear industry subject matter experts and specialists in the technical systems under consideration. Additionally, we will continue to pursue the system analyses initiated in the first part of our effort, with a particular emphasis on STPA as the main tool to identify weak or weakening control structures that affect the resilience of organizations and programs. Our intent is to broaden the scope of the analysis from an individual use case to a related set of use cases (e.g., maintenance tasks, compliance tasks) with similar human-system performance challenges. This will enable more generalized findings to refine the Proactive Issue Resolution and IAE models, as well as their system-level requirements and safety constraints. We will use organizational system modeling analyses to supplement STPA findings and model development. We conclude the report with a set of summary recommendations and an initial draft list of system-level requirements and safety constraints for optimized information automation systems.

99 GENERAL AND MISCELLANEOUS↗

Effect of mammalian mesopredator exclusion on vertebrate scavenging communities

Carrion is a valuable resource used by facultative scavengers across the globe. Due to conficts with humans, many vertebrate scavengers have experienced population declines due to direct persecution or indirect efects of human activities. However, little is known about the implications of altered scavenger community composition on the fate and efciency of carrion removal within ecosystems. In particular, mammalian mesopredators are efcient scavengers that are often subjected to control, thus, it is important to understand how the reduction of this scavenger guild infuences the fate of carrion resources and efciency of carrion removal within ecosystems. We evaluated the infuence of the absence of mammalian mesopredators on vertebrate scavenging dynamics by comparing the efciency of carrion removal and species composition at carrion between sites where we experimentally manipulated mesopredator abundance and paired control sites. Overall scavenging rates were high, even within our mesopredator exclusion sites (79% of carcasses). Despite the exclusion of an entire guild of dominant scavengers, we saw little efect on scavenging dynamics due to the extensive acquisition of carrion by avian scavengers. However, we observed a slight reduction in vertebrate scavenging efciency in sites where mesopredators were excluded. Our results suggest vertebrate communities are highly efcient at carrion removal, as we saw a functional response by avian scavengers to increased carrion availability. These data provide insights into the impact of mesopredator control on food web dynamics, and build upon the growing body of knowledge investigating the role of vertebrate scavengers on ecosystem services provided through carrion removal.

54 ENVIRONMENTAL SCIENCES↗