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At least 541 records · Page 30

An ecological niche model to predict the geographic distribution of Haemagogus janthinomys, Dyar, 1921 a yellow fever and Mayaro virus vector, in South America

Yellow fever virus (YFV) has a long history of impacting human health in South America. Mayaro virus (MAYV) is an emerging arbovirus of public health concern in the Neotropics and its full impact is yet unknown. Both YFV and MAYV are primarily maintained via a sylvatic transmission cycle but can be opportunistically transmitted to humans by the bites of infected forest dwelling Haemagogus janthinomys Dyar, 1921. To better understand the potential risk of YFV and MAYV transmission to humans, a more detailed understanding of this vector species’ distribution is critical. This study compiled a comprehensive database of 177 unique Hg . janthinomys collection sites retrieved from the published literature, digitized museum specimens and publicly accessible mosquito surveillance data. Covariate analysis was performed to optimize a selection of environmental (topographic and bioclimatic) variables associated with predicting habitat suitability, and species distributions modelled across South America using a maximum entropy (MaxEnt) approach. Our results indicate that suitable habitat for Hg . janthinomys can be found across forested regions of South America including the Atlantic forests and interior Amazon.

60 APPLIED LIFE SCIENCES↗

Microbial ecology and biogeochemistry of hypersaline sediments in Orca Basin

In deep ocean hypersaline basins, the combination of high salinity, unusual ionic composition and anoxic conditions represents significant challenges for microbial life. We used geochemical porewater characterization and DNA sequencing based taxonomic surveys to enable environmental and microbial characterization of anoxic hypersaline sediments and brines in the Orca Basin, the largest brine basin in the Gulf of Mexico. Full-length bacterial 16S rRNA gene clone libraries from hypersaline sediments and the overlying brine were dominated by the uncultured halophilic KB1 lineage, Deltaproteobacteria related to cultured sulfate-reducing halophilic genera, and specific lineages of heterotrophic Bacteroidetes. Archaeal clones were dominated by members of the halophilic methanogen genus Methanohalophilus, and the ammonia-oxidizing Marine Group I (MG-I) within the Thaumarchaeota. Illumina sequencing revealed higher phylum- and subphylum-level complexity, especially in lower-salinity sediments from the Orca Basin slope. Illumina and clone library surveys consistently detected MG-I Thaumarchaeota and halotolerant Deltaproteobacteria in the hypersaline anoxic sediments, but relative abundances of the KB1 lineage differed between the two sequencing methods. The stable isotopic composition of dissolved inorganic carbon and methane in porewater, and sulfate concentrations decreasing downcore indicated methanogenesis and sulfate reduction in the anoxic sediments. While anaerobic microbial processes likely occur at low rates near their maximal salinity thresholds in Orca Basin, long-term accumulation of reaction products leads to high methane concentrations and reducing conditions within the Orca Basin brine and sediments.

54 ENVIRONMENTAL SCIENCES↗

Caribbean deepwater snappers: Application of the bomb radiocarbon age estimation validation in understanding aspects of ecology and life history

Reef fishes have been utilized as food fish throughout the U.S. Caribbean and Gulf of Mexico waters for centuries, with increasing fishing effort in recent decades. As a result, many species have experienced declines in landings, including deepwater snappers such as queen snapper Etelis oculatus and blackfin snapper Lutjanus buccanella . However, little to no peer-reviewed published research exists on basic life history parameters for either species. Confirming the accuracy of an age estimation method for a fish species is essential for ensuring sustainable fisheries management. This is because in the assessment of fisheries species population age-based parameters, including longevity, age at sexual maturity, growth rate, mortality, age-specific reproductive output, and lifetime reproductive output, are important in understanding overall life history strategies of managed stocks. The past stock assessment on U.S. Caribbean queen snapper utilized an estimated longevity of 8 y, derived from length frequencies for fish from St. Lucia. Blackfin snapper has an estimated longevity of 27 y based on a relatively small study from offshore waters of the southeastern U.S. The focus of our investigation was to estimate maximum longevity of two data-poor species in the U.S. Caribbean. The accuracy of ageing methods was tested via bomb radiocarbon age estimation validation and effects of depth on Δ 14 C in otolith cores and eye lens core values were examined. Results from our work indicate a maximum validated age of 45 y for queen snapper, and 43 y for blackfin snapper. Our findings indicate queen snapper and blackfin snapper are long-lived (> 40 y). The resulting Δ 14 C comparison between eye lens cores and otolith cores has important implications for the study of age validation, specifically when deepwater species are involved.

59 BASIC BIOLOGICAL SCIENCES↗

Scat Detection Dog Surveys for the San Joaquin Kit Fox on the Lawrence Livermore National Laboratory's Experimental Test Site (Site 300) and the Corral Hollow Ecological Reserve: 2020 Deployment

The San Joaquin kit fox (Vulpes macrotis mutica) historically occupied an extensive range in the San Joaquin Valley, California; however, their populations and habitat have since been significantly reduced by human impacts (United States Fish and Wildlife Service [USFWS] 1998). More than 95% of the potential habitat for kit foxes on the San Joaquin Valley floor has been converted to irrigated agriculture, urbanized, or industrialized lands. The San Joaquin kit fox was listed as endangered by the USFWS in 1967 and as threatened by the State of California in 1971. Records from local surveys, research projects, and incidental sightings indicate the present distribution of kit foxes extends from 1) southern Kern County north to Contra Costa, Alameda, and San Joaquin counties on the west side of the valley and to Stanislaus County on the east side; 2) into some of the larger, uncultivated valley-floor land parcels in Kern, Tulare, Kings, Fresno, Madera, and Merced counties; and 3) westward within 5 counties in the interior coastal range (USFWS 1998). Kit foxes have been denoted to occur in 3 geographically distinct core and several satellite populations in a heavily fragmented landscape, with the largest extant populations concentrated in the southern part of the range and smaller populations and isolated sightings in the central and northern portions (USFWS 1998, 2010).

54 ENVIRONMENTAL SCIENCES↗

Evaluating the Long-Term Ecological Responses to Riparian Ecosystem Restoration at the Fort Benning, Georgia Military Installation

Riparian and instream restorations are commonly implemented to improve ecosystem condition. However, very few restoration activities are monitored for multiple years to assess long-term effectiveness. At the Department of Defense’s Fort Benning Military Installation (FBMI) in west-central Georgia, two experimental restoration projects (ephemeral drainage revitalization and instream habitat augmentation [i.e., coarse woody debris (CWD) additions]) were implemented ~14 years ago with the goal of reducing environmental impacts of military training activities. A SERDP project found that instream restoration improved some aspects of ecosystem function and community structure but not others. The objective of the current study was to re-evaluate the effect of these restoration practices on stream ecosystem processes ~14 years after implementation.

54 ENVIRONMENTAL SCIENCES↗

Microbial Ecology of the Plant Rhizosphere (PlantMicrobe)

The growth of bioenergy crops has the potential to help fulfill the nation’s demand for energy. However, growing these crops on arable lands may lead to socioeconomic concerns. One proposed solution is to grow biofuel crops in nutrient poor soils that aren’t well suited for food-crop growth. Therefore, in order to understand the impact of nutrient poor environments on plants, there is a need to investigate model plants and their rhizospheres in these environments using high throughput molecular analyses. Here the rhizosphere can be defined as the zone around underground portion of the plant that is influenced by the plant’s roots, and can include chemical, biological, and physical influences.

09 BIOMASS FUELS↗

Quantitative, trait-based microbial ecology to accurately model the impacts of nitrogen deposition on soil carbon cycling in the Anthropocene (Final Technical Report)

Atmospheric nitrogen pollution has altered fundamental soil processes, challenging our understanding of the extent to which soils will continue to sequester carbon and slow the pace of future environmental change. While most evidence shows that increases in soil nitrogen have enhanced carbon storage in temperate forests, it remains unclear whether these effects will persist as nitrogen pollution continues to decline. Moreover, there remains uncertainty as to why some forests gain more carbon in response to nitrogen pollution than others. At the heart of this knowledge gap is a failure to link nitrogen–induced shifts in microbial biodiversity with gains or losses in their functional ability to decompose, assimilate, and ultimately stabilize soil carbon. Given that this uncertainty impedes the ability of predictive models to project future soil carbon stocks, there is a critical need to determine how key microbial traits drive soil carbon responses to nitrogen pollution. As such the goals of this project were to: (1) Quantify variation in taxon–specific and community–level microbial traits across gradients in microbial community composition, mycorrhizal symbioses, and nitrogen availability. (2) Integrate empirical data into a novel predictive framework that enhances our ability to project the regional soil carbon consequences of historical nitrogen pollution in temperate forests.

54 ENVIRONMENTAL SCIENCES↗

The Risk Assessment Information System Compendium of Ecological Screening Benchmarks for Chemicals and Radionuclides (2025) (Volume VIII – Chemicals for Sediment: General)

Volume VIII presents the chemical benchmarks for sediment: general. The benchmarks here are applicable to both freshwater sediments and marine sediments or where no differentiation was made by the source. These values are not repeated in the freshwater sediment and marine sediment categories. Likewise, values listed in the freshwater sediment and marine sediment categories are not repeated in the generic categories. Consult Volume I for information on the sources.

54 ENVIRONMENTAL SCIENCES↗

The Risk Assessment Information System Compendium of Ecological Screening Benchmarks for Chemicals and Radionuclides (2025) (Volume V – Chemicals for Surface Water: General)

Volume V presents the chemical benchmarks for surface water: general. The benchmarks here are applicable to both fresh water and marine or where no differentiation was made by the source. These values are not repeated in the fresh water and marine categories. Likewise, values listed in the fresh water and marine categories are not repeated in the generic categories. Consult Volume I for information on the sources.

54 ENVIRONMENTAL SCIENCES↗

Metabolic model-based ecological modeling for probiotic design

The microbial community composition in the human gut has a profound effect on human health. This observation has lead to extensive use of microbiome therapies, including over-the-counter ‘probiotic’ treatments intended to alter the composition of the microbiome. Despite so much promise and commercial interest, the factors that contribute to the success or failure of microbiome-targeted treatments remain unclear. We investigate the biotic interactions that lead to successful engraftment of a novel bacterial strain introduced to the microbiome as in probiotic treatments. We use pairwise genome-scale metabolic modeling with a generalized resource allocation constraint to build a network of interactions between taxa that appear in an experimental engraftment study. We create induced sub-graphs using the taxa present in individual samples and assess the likelihood of invader engraftment based on network structure. To do so, we use a generalized Lotka-Volterra model, which we show has strong ability to predict if a particular invader or probiotic will successfully engraft into an individual’s microbiome. Furthermore, we show that the mechanistic nature of the model is useful for revealing which microbe-microbe interactions potentially drive engraftment.

59 BASIC BIOLOGICAL SCIENCES↗