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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 541 records · Page 30
All sourcesNASA NTRSDOE OSTI
DOE OSTI2022

Summer thaw duration is a strong predictor of the soil microbiome and its response to permafrost thaw in arctic tundra

Abstract Climate warming has increased permafrost thaw in arctic tundra and extended the duration of annual thaw (number of thaw days in summer) along soil profiles. Predicting the microbial response to permafrost thaw depends largely on knowing how increased thaw duration affects the composition of the soil microbiome. Here, we determined soil microbiome composition from the annually thawed surface active layer down through permafrost from two tundra types at each of three sites on the North Slope of Alaska, USA. Variations in soil microbial taxa were found between sites up to ~90 km apart, between tundra types, and between soil depths. Microbiome differences at a site were greatest across transitions from thawed to permafrost depths. Results from correlation analysis based on multi‐decadal thaw surveys show that differences in thaw duration by depth were significantly, positively correlated with the abundance of dominant taxa in the active layer and negatively correlated with dominant taxa in the permafrost. Microbiome composition within the transition zone was statistically similar to that in the permafrost, indicating that recent decades of intermittent thaw have not yet induced a shift from permafrost to active‐layer microbes. We suggest that thaw duration rather than thaw frequency has a greater impact on the composition of microbial taxa within arctic soils.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2024

Using ARM Data to Retrieve Entrainment Rates in Stratocumulus Cloud Systems (Final Report)

A stratocumulus-topped boundary layer (STBL) is a shallow convecting layer in which both the updraft and the downdraft branches of the convective circulation are saturated (cloudy) in the upper part of the boundary layer. The convective circulation is vigorous, yet the cloud-top entrainment velocity, $w_e$, is small and difficult to measure. Differences between currently used parameterizations of $w_e$ in GCMs can lead to STBLs that differ by as much as a factor of two in climatologically important properties such as liquid water path and boundary layer depth. We have developed a new method to retrieve entrainment rates in STBLs which uses vertical velocity retrievals from surface-based cloud radar, in conjunction with other surface-based measurements. These new estimates will allow a more comprehensive evaluation of parameterizations of entrainment rate for STBLs. The new method will also provide ground-based estimates of entrainment in STBLs over long time periods. We use retrievals of vertical velocity and liquid water content profiles to estimate the turbulent liquid water flux, which is then used in combination with observed cloud-top jumps of temperature, water vapor, and calculated radiative flux profiles to estimate the entrainment rate. We present results for three days for which observational data was not significantly contaminated by drizzle. The resulting average entrainment rates are 0.58 cm/s, 1.51 cm/s, and 0.31 cm/s.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2021

Geophysical and Environmental Monitoring Data, Lower Watershed, Teller Road Mile Marker 27, Seward Peninsula, Alaska, 2017-2019

This data set contains geophysical and environmental monitoring data acquired between September 2017 and 2019 at the lower Teller watershed, Seward Peninsula, Alaska. Geophysical data comprises processed resistivity data, acquired daily between Spring and Fall of 2018 and 2019, and a baseline measurement of Fall 2017. The environmental monitoring data comprises depth resolved, distributed soil moisture and soil temperature data. These measurements were obtained used Decagon 5TE sensors placed at 0.1 m, 0.2 m , 0.3 m, and 0.4 m depth. In addition, temperature data at 0.5 m, 1.0 m, and 1.5 m were acquired using Hobo temperature sensors. Data were collected to improve our understanding of the hydrological response of discontinuous permafrost systems, in particular focusing on multi-annual dynamics and short term disturbances, such as snowmelt or precipitation events. The electrical resistivity tomography (ERT) monitoring data are included as processed resistivity models, with model cells x dimension equal to the distance along the profile, and model z dimension being elevation. The start and end point of the transect are (UTM Zone 3N): E 454881.66 m, N 7178949.45 m, and E 454772.60, N 7178885.81. Locations of the soil moisture and temperature sensors are provided in the data package. This dataset is discussed in detail in the Uhlemann, S. et al 2021 paper listed in the references. This dataset includes one *.pdf user guide and 315 *.csv data files included within four zipped files.The Next-Generation Ecosystem Experiments: Arctic (NGEE Arctic), was a research effort to reduce uncertainty in Earth System Models by developing a predictive understanding of carbon-rich Arctic ecosystems and feedbacks to climate. NGEE Arctic was supported by the Department of Energy's Office of Biological and Environmental Research.The NGEE Arctic project had two field research sites: 1) located within the Arctic polygonal tundra coastal region on the Barrow Environmental Observatory (BEO) and the North Slope near Utqiagvik (Barrow), Alaska and 2) multiple areas on the discontinuous permafrost region of the Seward Peninsula north of Nome, Alaska.Through observations, experiments, and synthesis with existing datasets, NGEE Arctic provided an enhanced knowledge base for multi-scale modeling and contributed to improved process representation at global pan-Arctic scales within the Department of Energy's Earth system Model (the Energy Exascale Earth System Model, or E3SM), and specifically within the E3SM Land Model component (ELM).

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2025

Marine and continental stratocumulus cloud microphysical properties obtained from routine ARM Cimel sunphotometer observations

This study investigates marine and continental stratocumulus (Sc) cloud properties obtained from an automated implementation of a multispectral photometer retrieval. Photometer methods simultaneously retrieve cloud optical depth (τ) and cloud droplet effective radius (r e ), with estimates for liquid water path (LWP) calculated on the availability of those quantities. These applied methods evaluate retrieved cloud properties for Sc identified during a recent 6 year period over the U.S. Department of Energy Atmospheric Radiation Measurement (ARM) program sites in Oklahoma, USA (SGP) and in the Azores, Portugal (ENA). Modest agreement in key quantity retrievals is found between the routine photometer products and multisensor collocated profiling references. Cumulative breakdowns contingent on cloud thickness indicate increases in all retrieved quantities in thicker clouds, with larger discrepancies in the relative performance between the retrievals collected in the presence of drizzle. Under continental cloud conditions, the clouds of a similar thickness and r e to those sampled under marine conditions report a factor of 1.5 larger τ and LWP. An r 2 ≅0.65 is found between photometer τ retrievals and shadowband radiometer measurements, with photometer retrievals reporting a high (relative) bias. The τ intercomparisons indicate that variability between retrievals is a factor of three larger than errors reported from individual retrieval input perturbation tests. Photometer r e retrievals suggest a low r 2 (< 0.1) having a standard deviation ≅ 3 µm when compared to ARM baseline multi-sensor radar/radiometer references (accounting for offsets in the cloud droplet number concentration assumptions of the latter). However, photometer LWP calculations remain relatively unbiased in non-drizzling conditions, with errors O (50 g m −2 ) and r 2 ≅0.5 to collocated radiometer and interferometer references. Additional sensitivity tests for island influences on marine Sc properties suggest that while island-influenced winds may promote larger cloud LWP or thickness, the influence could be within retrieval method uncertainty and/or collocated instrument variability.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2021

Simultaneous x-ray and neutron 4D tomographic study of drying-driven hydro-mechanical behavior of cement-based materials at moderate temperatures

The drying of cement-based materials is intimately related to their durability, which has significant economic, social and environmental repercussions. The evolution of the saturation of the pore network and the associated drying shrinkage are in fact leading causes of cracking and of the ensuing penetration of aggressive chemicals. This process is highly heterogeneous, due to the thermo-hydric spatial gradients developing in the material from the exposed surfaces to its core and because of local effects, driven by the intrinsically heterogeneous micro-structure (e.g., by the distribution of pores and aggregates). It follows that macroscopic, sample-scale measurements cannot fully disclose the complexity of the underlying processes. In the last few decades, significant advances in full-field techniques have allowed an unprecedented insight into these local processes. For cement-based materials, x-ray and neutron tomography lend themselves as ideal, and highly complementary, tools for the study of their thermo-hydro-mechanical behavior. Notably, the high sensitivity to density variations of x-ray imaging gives access to the developments of fractures, in 4D (3D + time). On the other hand, neutron tomography allows the study of the evolution of the moisture field in 4D, thanks to its high hydrogen sensitivity. The combination of these two techniques provides a unique insight in thermo-hydro-mechanical couplings, e.g., the effect of cracks on the water content field. This contribution presents novel 5D datasets (3D tomographies along time, plus truly simultaneous x-ray and Neutron rapid acquisitions) in-operando of a cement paste and of a concrete sample heated at moderate temperatures (up to 140{sup ∘}C). The analysis of this 5D data-set (once aligned in time and across modalities) allows for example a quantification of the 4D moisture profiles which were found to predict an overall water loss at hydric equilibrium coherent with the corresponding analytical analysis. In the cement paste sample, the x-ray dataset captures the evolution of an extensive cracking network, opening and propagation toward the core of the sample. A novel analysis procedure is here proposed which allows the extraction of these fractures and the analysis of their interplay with local drying as captured through neutron imaging. This for example reveals the depth of penetration of drying in the vicinity of the fractures along time, which is essential for the assessment and calibration of hydro-mechanical coupled models.

36 MATERIALS SCIENCE↗
DOE OSTI2024

Thermal and Well Flow Performance of Closed-Loop Geothermal in the Wattenberg Area: Preprint

Closed-loop geothermal systems provide an alternative to resource-constrained hydrothermal systems and stimulation-intensive enhanced geothermal systems. In this work, we apply the slender-body theory (SBT) model, to simulate the well flow and heat transfer performance of U-loop well designs drilled in the Wattenberg area of the Denver-Julesburg Basin. Three U-loop well patterns are investigated including a single, double, and multi-lateral design. The subsurface within area is characterized by deep, hot (> 200degreesC) igneous/metamorphic basement rock underlying multiple sedimentary formations. The lateral section(s) of the U-loop lies within a target depth of 6 km where temperatures are estimated to approach 300degreesC. As a base case, conduction-only heat transfer is investigated through simulations with the SBT model within U-loops with open-hole laterals that exchange heat directly with the hot dry rock using water as a working fluid. The utilization of supercritical CO2 as a heat transfer fluid is also considered. For each scenario, the system performance in terms of annual heat production and temperature profile over a 20-year project lifetime are assessed. Also, the levelized costs of heat and electricity (LCOH and LCOE) are determined using a top-down technoeconomic analysis model. The results show that the performance and cost optimized U-loop design is one having an injection-production well spacing of 1,000 meters with ten 50-meter-spaced laterals that traverse a subsurface system with a temperature gradient of 60degreesC/km. By injecting 20 degreesC-water at a rate of 60 kg/s through this loop, an average heat production of 19 MWth can be achieved, resulting in an LCOE and LCOH of $136/MWh and $1.53/GJ, respectively, over a 20-year project life.

closed-loop geothermal↗
DOE OSTI2023

Influence of soil depth, irrigation, and plant genotype on the soil microbiome, metaphenome, and carbon chemistry

ABSTRACT Climate change is causing an increase in drought in many soil ecosystems and a loss of soil organic carbon. Calcareous soils may partially mitigate these losses via carbon capture and storage. Here, we aimed to determine how irrigation-supplied soil moisture and perennial plants impact biotic and abiotic soil properties that underpin deep soil carbon chemistry in an unfertilized calcareous soil. Soil was sampled up to 1 m in depth from irrigated and planted field treatments and was analyzed using a suite of omics and chemical analyses. The soil microbial community composition was impacted more by irrigation and plant cover treatments than by soil depth. By contrast, metabolomes, lipidomes, and proteomes differed more with soil depth than treatments. Deep soil (>50 cm) had higher soil pH and calcium concentrations and higher levels of organic acids, bicarbonate, and triacylglycerides. By contrast, surface soil (0–5 cm) had higher concentrations of soil organic matter, organic carbon, oxidizable carbon, and total nitrogen. Surface soils also had higher amounts of sugars, sugar alcohols, phosphocholines, and proteins that reflect osmotic and oxidative stress responses. The lipidome was more responsive to perennial tall wheatgrass treatments compared to the metabolome or proteome, with a striking change in diacylglyceride composition. Permanganate oxidizable carbon was more consistently correlated to metabolites and proteins than soil organic and inorganic carbon and soil organic matter. This study reveals specific compounds that reflect differences in organic, inorganic, and oxidizable soil carbon fractions that are impacted by interactions between irrigation-supplied moisture and plant cover in calcareous soil profiles. IMPORTANCE Carbon is cycled through the air, plants, and belowground environment. Understanding soil carbon cycling in deep soil profiles will be important to mitigate climate change. Soil carbon cycling is impacted by water, plants, and soil microorganisms, in addition to soil mineralogy. Measuring biotic and abiotic soil properties provides a perspective of how soil microorganisms interact with the surrounding chemical environment. This study emphasizes the importance of considering biotic interactions with inorganic and oxidizable soil carbon in addition to total organic carbon in carbonate-containing soils for better informing soil carbon management decisions.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2022

Machine learning and atomic layer deposition: Predicting saturation times from reactor growth profiles using artificial neural networks

In this work, we explore the application of deep neural networks to the optimization of atomic layer deposition (ALD) processes. In particular, we focus on a one-shot optimization problem, where we try to predict the optimal dose time that leads to saturation everywhere in the reactor based on thickness values measured at different points of an ALD reactor after a single trial growth. In order to tackle this problem, we introduce a dataset designed to train neural networks to predict saturation times based on these inputs for a cross-flow ALD reactor. Here, we then explore the predictive ability of artificial neural networks of different depths and sizes using a separate testing dataset to evaluate their accuracies. The results obtained show that networks trained using stochastic gradient descent methods can accurately predict saturation times without requiring any additional information on the surface kinetics. This provides a viable approach to minimize the number of experiments required to optimize new ALD processes in a known reactor, and it highlights the way machine learning can be leveraged for thin film growth and manufacturing. While the datasets and training procedure depend on the reactor geometry, the trained neural networks provide a general surrogate model connecting thickness values and trial dose times with optimal saturation times that can be reused for different ALD processes within the same reactor.

36 MATERIALS SCIENCE↗
DOE OSTI2022

Soil C stock and CO2 production data for Soong et al. 2021: Effects of five years of soil warming at Blodgett Forest, CA.

Subsoils below 20 cm are an important reservoir in the global carbon cycle, but little is known about their vulnerability under climate change. We measured subsoil carbon in warmed vs control plots of a conifer forest after 4.5 years of whole-soil warming (4°C). We also measured soil CO2 efflux at the surface and soil CO2 concentrations along the soil profile. Over the 4.5 years we observed that warming resulted in a loss of subsoil carbon stocks (−33 ± 11%), primarily from unprotected particulate organic matter. Warming also stimulated a sustained 30 ± 4% increase in soil CO2 efflux due to increased CO2 production through the whole-soil profile. The observed in situ decline in subsoil carbon stocks with warming is now definitive evidence of a positive soil carbon-climate feedback, which could not be concluded based on increases in CO2 effluxes alone. This dataset comprises (1) 13C and C recovery in 2014, 2016, and 2018 1m soil cores sampled in 10cm increments. (2) Monthly surface CO2 fluxes from 2014 to 2018. (3) Monthly gas well sample [CO2] and production data. And (4) density fraction data for three depths of cores recovered in 2017.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2025

Thermal and Well Flow Performance of Closed-Loop Geothermal in the Wattenberg Area

Closed-loop geothermal systems provide an alternative to resource-constrained hydrothermal systems and stimulation-intensive enhanced geothermal systems. In this work, we apply the slender-body theory (SBT) model, to simulate the well flow and heat transfer performance of U-loop well designs in the Wattenberg area of the Denver-Julesburg Basin. Three U-loop well patterns are investigated, including a single-, double-, and multi-lateral design. The subsurface within the area of interest is characterized by deep, hot (> 200 degrees C) igneous/metamorphic basement rock underlying multiple sedimentary formations. The lateral section(s) of the U-loop lie(s) within a target depth of 6 km, where temperatures are estimated to approach 300 degrees C. As a base case, conduction-only heat transfer is investigated through simulations with the SBT model within U-loops with open-hole laterals that exchange heat directly with the hot, dry rock using water as a working fluid. The utilization of supercritical CO2 as a heat transfer fluid is also considered. For each scenario, the system performance in terms of annual heat production and temperature profile over a 20-year project lifetime are assessed. Also, the levelized costs of heat and electricity (LCOH and LCOE) are determined using a top-down techno-economic analysis model. The results show that the performance- and cost-optimized U-loop design is one having an injection-production well spacing of 1,000 meters with ten 50-meter-spaced laterals that traverse a subsurface system with a temperature gradient of 60 degrees C/km. By injecting 20 degrees C-water at a rate of 60 kg/s through this loop, an average heat production of 19 MWth (i.e., 2.2 MWe net plant output) can be achieved, resulting in an LCOE and LCOH of $136/MWhe and $1.53/GJ, respectively, over a 20-year project life.

closed-loop geothermal↗
DOE OSTI2023

Dissecting the structural heterogeneity of proteins by native mass spectrometry

Abstract A single gene yields many forms of proteins via combinations of posttranscriptional/posttranslational modifications. Proteins also fold into higher‐order structures and interact with other molecules. The combined molecular diversity leads to the heterogeneity of proteins that manifests as distinct phenotypes. Structural biology has generated vast amounts of data, effectively enabling accurate structural prediction by computational methods. However, structures are often obtained heterologously under homogeneous states in vitro. The lack of native heterogeneity under cellular context creates challenges in precisely connecting the structural data to phenotypes. Mass spectrometry (MS) based proteomics methods can profile proteome composition of complex biological samples. Most MS methods follow the “bottom‐up” approach, which denatures and digests proteins into short peptide fragments for ease of detection. Coupled with chemical biology approaches, higher‐order structures can be probed via incorporation of covalent labels on native proteins that are maintained at the peptide level. Alternatively, native MS follows the “top‐down” approach and directly analyzes intact proteins under nondenaturing conditions. Various tandem MS activation methods can dissect the intact proteins for in‐depth structural elucidation. Herein, we review recent native MS applications for characterizing heterogeneous samples, including proteins binding to mixtures of ligands, homo/hetero‐complexes with varying stoichiometry, intrinsically disordered proteins with dynamic conformations, glycoprotein complexes with mixed modification states, and active membrane protein complexes in near‐native membrane environments. We summarize the benefits, challenges, and ongoing developments in native MS, with the hope to demonstrate an emerging technology that complements other tools by filling the knowledge gaps in understanding the molecular heterogeneity of proteins.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2024

Thermal Equations of State of Magnesite: Implication for the Complex Mid‐Lower Mantle Seismic Scatterers

Abstract Magnesite (MgCO 3 ) entering the lower mantle together with the subducted oceanic crust is an important carbon carrier. The reaction between magnesite and mantle minerals has been documented, but its influence on the density and velocity profiles of lower mantle remains unexplored. To decipher the deep carbon transportation and its associated effect, here we determined the thermal equations of state of magnesite up to 120 GPa and 2600 K using X‐ray diffraction in laser‐heated diamond anvil cells. The obtained thermal elastic parameters of magnesite facilitated a comprehensive understanding on the influence of magnesite‐SiO 2 reaction, variation of carbon and SiO 2 content, and temperature on the origin of lower‐mantle scatterers at 1,000–1,800 km depth. Our modeling revealed that the depth of the lower‐mantleV S scatterers is mainly controlled by the Al 2 O 3 content in SiO 2 , while its magnitude depends on the SiO 2 content. Along normal geotherm, the magnesite‐SiO 2 reaction would occur before the post‐stishovite transition, consuming substantial SiO 2 in the subducted oceanic crust. Depending on the amount of residual SiO 2 , the post‐stishovite transition can produce a 2.5–5.2 (2)%V S reduction, compatible with the observed seismic scatterers in Izu‐Bonin and Mariana subduction zones. Along slab geotherm, this reaction occurs after the post‐stishovite transition, generating a greaterV S reduction of 4.4–6.4 (4)%. We thus propose that the reaction between sinking MgCO 3 and SiO 2 in the slab is one of the potential factors influencing the magnitude of the lower‐Vs scatterers at 1,000–1,900 km depth. Our results provide new insights into the deep‐mantle carbonate transportation influencing regional geophysics.

Geochemistry & Geophysics↗
DOE OSTI2022

Self-similarity of scalar isosurface area density in a temporal mixing layer

Understanding the behaviour of scalar isosurfaces in a turbulent flow is of particular interest for many problems in turbulent mixing that contain sharp interfaces between regions of the flow. Common examples include combustion, where the chemical reactions occur in thin regions within the flow, and the turbulent/non-turbulent interface in shear flows, where a thin region separates the rotational, turbulent motions from the irrotational, non-turbulent background. Recent advances in computing technology allow for in-depth analysis of these interface problems that are difficult to quantify in a laboratory setting. In this paper, the results of a direct numerical simulation of a passive scalar $\varPhi$ evolving on a turbulent, temporally developing mixing layer are described. A novel approach has been taken to calculate the surface area of individual scalar isosurfaces, $A_{iso}$ , throughout the simulation, as well as the mean isosurface area density, $\varSigma$ , as a function of the cross-stream direction and time. A notable finding is that the profiles of $\varSigma$ develop in a self-similar manner when scaled by the Taylor scale of the scalar field, $\lambda _\phi$ . Remarkably, the scaling appears to hold for a wide range of isovalues. A rough scaling argument based on the formal definition of $\varSigma$ and properties of a temporal mixing layer is presented which also exposes a dependence on $\lambda _\phi$ . Based on these results, a possible scaling for the isosurface area is presented as $A_{iso}/A_0 \sim (Re \, Sc)^{1/2}$ , where $Re$ and $Sc$ are local Reynolds and Schmidt numbers, respectively.

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗
DOE OSTI2025

Porewater chemistry in Typha-dominated brackish tidal marsh, PIE LTER, Plum Island Sound, MA, July 2022–September 2024

This dataset contains profile measurements of porewater constituents taken on 3-4 days across the growing seasons in 2022, 2023, and 2024 in a tidal brackish marsh within the Plum Island Ecosystems Long Term Ecological Research site (PIE LTER), located in the Plum Island Sound, Massachusetts (MA). Measurements were taken to monitor changes in porewater chemistry induced by seasonal saltwater intrusion at the site. Samples were taken in two locations: one was close to the creek bank and the other in the marsh interior. Water was sampled from 2-5 depths between the surface to 50cm using a sipper consisting of a hollow stainless steel rod with an opening at the end similar to that described in (Berg & McGlathery, 2001). The rod was pushed into the sediment to the desired depth, typically every 10cm, and water samples were taken by syringe. Water was not obtained at all depths. Samples were preserved and analyzed in the lab. Metadata files Typha_porewater_sipper_dd.csv and Typha_porewater_sipper_flmd.csv contain detailed information on data variables, sampling and QA/QC methods, and site location.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2020

Impacts of Arctic Shrubs on Root Traits and Belowground Nutrient Cycles Across a Northern Alaskan Climate Gradient

Deciduous shrubs are expanding across the graminoid-dominated nutrient-poor arctic tundra. Absorptive root traits of shrubs are key determinants of nutrient acquisition strategy from tundra soils, but the variations of shrub root traits within and among common shrub genera across the arctic climatic gradient are not well resolved. Consequently, the impacts of arctic shrub expansion on belowground nutrient cycling remain largely unclear. Here, we collected roots from 170 plots of three commonly distributed shrub genera ( Alnus , Betula , and Salix ) and a widespread sedge ( Eriophorum vaginatum ) along a climatic gradient in northern Alaska. Absorptive root traits that are relevant to the strategy of plant nutrient acquisition were determined. The influence of aboveground dominant vegetation cover on the standing root biomass, root productivity, vertical rooting profile, as well as the soil nitrogen (N) pool in the active soil layer was examined. We found consistent root trait variation among arctic plant genera along the sampling transect. Alnus and Betula had relatively thicker and less branched, but more frequently ectomycorrhizal colonized absorptive roots than Salix , suggesting complementarity between root efficiency and ectomycorrhizal dependence among the co-existing shrubs. Shrub-dominated plots tended to have more productive absorptive roots than sedge-dominated plots. At the northern sites, deep absorptive roots (>20 cm depth) were more frequent in birch-dominated plots. We also found shrub roots extensively proliferated into the adjacent sedge-dominated plots. The soil N pool in the active layer generally decreased from south to north but did not vary among plots dominated by different shrub or sedge genera. Our results reveal diverse nutrient acquisition strategies and belowground impacts among different arctic shrubs, suggesting that further identifying the specific shrub genera in the tundra landscape will ultimately provide better predictions of belowground dynamics across the changing arctic.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2020

Policy-Driven Sustainable Saline Drainage Disposal and Forage Production in the Western San Joaquin Valley of California

Environmental policies to address water quality impairments in the San Joaquin River of California have focused on the reduction of salinity and selenium-contaminated subsurface agricultural drainage loads from westside sources. On 31 December 2019, all of the agricultural drainage from a 44,000 ha subarea on the western side of the San Joaquin River basin was curtailed. This policy requires the on-site disposal of all of the agricultural drainage water in perpetuity, except during flooding events, when emergency drainage to the River is sanctioned. The reuse of this saline agricultural drainage water to irrigate forage crops, such as ‘Jose’ tall wheatgrass and alfalfa, in a 2428 ha reuse facility provides an economic return on this pollutant disposal option. Irrigation with brackish water requires careful management to prevent salt accumulation in the crop root zone, which can impact forage yields. The objective of this study was to optimize the sustainability of this reuse facility by maximizing the evaporation potential while achieving cost recovery. This was achieved by assessing the spatial and temporal distribution of the root zone salinity in selected fields of ‘Jose’ tall wheatgrass and alfalfa in the drainage reuse facility, some of which have been irrigated with brackish subsurface drainage water for over fifteen years. Electromagnetic soil surveys using an EM-38 instrument were used to measure the spatial variability of the salinity in the soil profile. The tall wheatgrass fields were irrigated with higher salinity water (1.2–9.3 dS m -1 ) compared to the fields of alfalfa (0.5–6.5 dS m -1 ). Correspondingly, the soil salinity in the tall wheatgrass fields was higher (12.5 dS m -1 –19.3 dS m -1 ) compared to the alfalfa fields (8.97 dS mm -1 –14.4 dS mm -1 ) for the years 2016 and 2017. Better leaching of salts was observed in the fields with a subsurface drainage system installed (13–1 and 13–2). The depth-averaged root zone salinity data sets are being used for the calibration of the transient hydro-salinity computer model CSUID-ID (a one-dimensional version of the Colorado State University Irrigation Drainage Model). This user-friendly decision support tool currently provides a useful framework for the data collection needed to make credible, field-scale salinity budgets. In time, it will provide guidance for appropriate leaching requirements and potential blending decisions for sustainable forage production. This paper shows the tie between environmental drainage policy and the role of local governance in the development of sustainable irrigation practices, and how well-directed collaborative field research can guide future resource management.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2022

Metabolic activity and community structure of prokaryotes associated with particles in the twilight zone of the South China Sea

The twilight zone is an important depth of the ocean where particulate organic matter (POM) remineralization takes place, and prokaryotes contribute to more than 70% of the estimated remineralization. However, little is known about the microbial community and metabolic activity associated with different particles in the twilight zone. The composition and distribution of particle-attached prokaryotes in the twilight zone of the South China Sea (SCS) were investigated using high-throughput sequencing and quantitative PCR, together with the Biolog Ecoplate™ microplates culture to analyze the microbial metabolic activity. We found that α- and γ-Proteobacteria dominating at the lower and upper boundary of the twilight zone, respectively; Methanosarcinales and Halobacteriales of the Euyarchaeota occupied in the larger particles at the upper boundary. Similar microbial community existed between euphotic layer and the upper boundary. Higher amount of shared Operational Taxonomic Units (OTUs) in the larger particles along the water depths, might be due to the fast sinking and major contribution of carbon flux of the larger particles from the euphotic layer. In addition to polymers as the major carbon source, carbohydrates and amino acids were preferentially used by microbial community at the upper and lower boundary, respectively. This could potentially be attributed to the metabolic capabilities of attached microbial groups in different particles, and reflected the initial preference of the carbon source by the natural microbes in the twilight zone as well. The microbial structure and carbon metabolic profiles could be complemented with metatranscriptomic analysis in future studies to augment the understanding of the complex carbon cycling pathways in the twilight zone.

Liu, Hao↗