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At least 541 records · Page 30

Preliminary Design of a Downstream Processing System for Protein Production in Space

Biomanufacturing is a promising technology to convert in situ resources into essential products including enzymes, therapeutics, biopolymers and other chemicals required to support deep-space missions that may not be easily supplied or produced by alternative means. In addition to the biomass production operations, vital down-stream steps including biomass harvesting/concentration, cell lysis, protein capture and purification are needed to produce an application-ready product. Commercially available terrestrial processes commonly require complex, heavy equipment and highly trained operators, which are not practical in deep space environments. In this work, we aim to identify approaches required to produce an intracellular, His-tagged recombinant enzyme using E. coli at 1 L production scales within the constraints of a deep-space mission as a model use-case scenario. Based on extensive literature review and commercially available products, we identified candidate technologies and products that could be integrated for deep space biomanufacturing. Different preliminary designs were then compared in terms of total system impacts on up-mass, processing time, and consumables required. Our analysis indicated that a biomass concentrator would significantly reduce the processing time and consumables required for the overall system without a large increase in the total mass. We also identified viable technologies for other steps such as cell lysis and protein purification. Predictions from our trade study will be validated in the laboratory by testing the most promising products with the results used to optimize the design. This research will help transfer technology that is well developed on Earth to a space-ready format to produce biological products from a wide variety of microorganisms that can support deep-space missions.

Biomanufacturing↗

Limiting Future Collision Risk to Spacecraft: An Assessment of NASA's Meteoroid and Orbital Debris Programs

Over the past 50 years, various NASA communities have contributed significantly to maturing NASA s meteoroid and orbital debris (MMOD)1 programs to their current state. As a result of these community efforts, and to NASA s credit, NASA s MMOD programs and models are now widely used and respected by the providers and users of both government and commercial satellites, nationally as well as internationally. Satellites have been redesigned to protect critical components from MMOD damage by moving critical components from exterior surfaces to deep inside a satellite s structure. Orbits are monitored and altered to minimize the risk of collision with tracked orbital debris. MMOD shielding added to the International Space Station (ISS) protects critical components and astronauts from potentially catastrophic damage that might result from smaller, untracked debris and meteoroid impacts. The space shuttle, as it orbited Earth, and whether docked to the ISS or not, was optimally oriented to protect its fragile thermal protection and thermal radiation systems from MMOD damage. In addition, astronauts inspected its thermal protection system for MMOD damage before the shuttle reentered Earth s atmosphere; Orion, NASA s capsule to carry astronauts to low Earth orbit, includes designs to mitigate the threat of MMOD damage and provide increased safety to the crew. When a handful of reasonable assumptions are used in NASA s MMOD models, scenarios are uncovered that conclude that the current orbital debris environment has already reached a "tipping point." That is, the amount of debris - in terms of the population of large debris objects, as well as overall mass of debris in orbit - currently in orbit has reached a threshold where it will continually collide with itself, further increasing the population of orbital debris. This increase will lead to corresponding increases in spacecraft failures, which will only create more feedback into the system, increasing the debris population growth rate. The increase thus far has been most rapid in low Earth orbit (LEO), with geosynchronous Earth orbits (GEOs) potentially suffering the same fate, but over a much longer time period. The exact timing and pace of this exponential growth are uncertain, but the serious implications of such a scenario require careful attention because of the strategic importance of U.S. space operations. The Office of Science and Technology Policy and the Office of Management and Budget contracted with the National Research Council for a study to perform three tasks: review NASA s MMOD programs and efforts, recommend in which of those NASA should increase or decrease its effort or change focus, and determine whether NASA should pursue work in any new MMOD areas. The official letter requesting the study and the full statement of task for the Committee for the Assessment of NASA s Orbital Debris Programs are in Appendixes A and B, respectively.

Source record↗

Energy and Mass Transport of Magnetospheric Plasmas during the November 2003 Magnetic Storm

Intensive energy and mass transport from the solar wind across the magnetosphere boundary is a trigger of magnetic storms. The storm on 20-21 November 2003 was elicited by a high-speed solar wind and strong southward component of interplanetary magnetic field. This storm attained a minimum Dst of -422 nT. During the storm, some of the solar wind particles enter the magnetosphere and eventually become part of the ring current. At the same time, the fierce solar wind powers strong outflow of H+ and O+ from the ionosphere, as well as from the plasmasphere. We examine the contribution of plasmas from the solar wind, ionosphere and plasmasphere to the storm-time ring current. Our simulation shows, for this particular storm, ionospheric O+ and solar wind ions are the major sources of the ring current particles. The polar wind and plasmaspheric H+ have only minor impacts. In the storm main phase, the strong penetration of solar wind electric field pushes ions from the geosynchronous orbit to L shells of 2 and below. Ring current is greatly intensified during the earthward transport and produces a large magnetic depression in the surface field. When the convection subsides, the deep penetrating ions experience strong charge exchange loss, causing rapid decay of the ring current and fast initial storm recovery. Our simulation reproduces very well the storm development indicated by the Dst index.

Fok, Mei-Chging↗

Neuroimmune responses to space radiation

One of the main health risks in human deep space exploration is central nervous system (CNS) damage by ionizing radiation due to exposure to galactic cosmic rays (GCRs). In animal models, irradiation with simulated GCRs or their components has been shown to cause neurodegeneration and neuroinflammation associated with cognitive and behavioral dysfunction. The extent of CNS damage is partially mediated by the blood-brain barrier (BBB), which regulates the interaction between CNS and systemic responses to stressors in the rest of the body. The main cellular regulators of BBB permeability are astrocytes, which also modulate neuronal death, neuroinflammation and oxidative stress. However, studies on BBB and astrocyte functions in regulating CNS responses to ionizing radiation have been limited, especially in human tissue/organ analogs. Therefore, we developed a high-throughput 3D organ-on-a-chip system to study human CNS and BBB impairments caused by deep space radiation. We investigated both immediate and delayed CNS responses to major GCR components: 0.3-0.8Gy 600MeV/n 56Fe ions; as well as to 0.5-1Gy X-rays. We observed ionizing radiation-mediated increases in BBB permeability that was exacerbated by astrocyte presence and accompanied by damage to endothelial cells and tight junctions, altered cytokine expression including TNFalpha upregulation, and increased oxidative stress. In particular, 600MeV/n 56Fe particle irradiation selectively induced astrocyte damage and increased blood-brain barrier permeability only in models that contained astrocytes in addition to endothelial cells, indicating astrocytes as a particularly radiosensitive component of the CNS that could therefore be a suitable a target for neuroprotection. Future studies will compare human and mouse CNS model responses to simulated GCRs and evaluate the induction of an anti-inflammatory phenotype in astrocytes as a potential countermeasure. Furthermore, in our lab we have been exploring the individual variability, genomic associations and secreted biomarkers of responses to space radiation, which could eventually be combined to address personalized CNS health risk and develop individual countermeasures. Ultimately, we aim to expand upon these results to uncover novel cellular and mechanistic targets for countermeasure development to mitigate human CNS damage in deep space exploration.

space radiation↗

Impacts of Soil-aquifer Heat and Water Fluxes on Simulated Global Climate

Climate models have traditionally only represented heat and water fluxes within relatively shallow soil layers, but there is increasing interest in the possible role of heat and water exchanges with the deeper subsurface. Here, we integrate an idealized 50m deep aquifer into the land surface module of the GISS ModelE general circulation model to test the influence of aquifer-soil moisture and heat exchanges on climate variables. We evaluate the impact on the modeled climate of aquifer-soil heat and water fluxes separately, as well as in combination. The addition of the aquifer to ModelE has limited impact on annual-mean climate, with little change in global mean land temperature, precipitation, or evaporation. The seasonal amplitude of deep soil temperature is strongly damped by the soil-aquifer heat flux. This not only improves the model representation of permafrost area but propagates to the surface, resulting in an increase in the seasonal amplitude of surface air temperature of >1K in the Arctic. The soil-aquifer water and heat fluxes both slightly decrease interannual variability in soil moisture and in landsurface temperature, and decrease the soil moisture memory of the land surface on seasonal to annual timescales. The results of this experiment suggest that deepening the modeled land surface, compared to modeling only a shallower soil column with a no-flux bottom boundary condition, has limited impact on mean climate but does affect seasonality and interannual persistence.

soil↗

A theory of rotating stall of multistage axial compressors

A theoretical analysis was made of rotating stall in axial compressors of many stages, finding conditions for a permanent, straight-through traveling disturbance, with the steady compressor characteristic assumed known, and with simple lag processes ascribed to the flows in the inlet, blade passages, and exit regions. For weak disturbances, predicted stall propagation speeds agree well with experimental results. For a locally-parabolic compressor characteristic, an exact nonlinear solution is found and discussed. For deep stall, the stall-zone boundary is most abrupt at the trailing edge, as expected. When a complete characteristic having unstalling and reverse-flow features is adopted, limit cycles governed by a Lienard's equation are found. Analysis of these cycles yields predictions of recovery from rotating stall; a relaxation oscillation is found at some limiting flow coefficient, above which no solution exists. Recovery is apparently independent of lag processes in the blade passages, but instead depends on the lags originating in the inlet and exit flows, and also on the shape of the given characteristic diagram. Small external lags and tall diagrams favor early recovery. Implications for future research are discussed.

Moore, F. K.↗

The Amazon and climate

The climatologies of cloudiness and precipitation for the Amazon, are reviewed and the physical causes of some of the observed features and those which are not well known are explained. The atmospheric circulation over the Amazon is discussed on the large scale tropical circulations forced by deep diabatic heating sources. Weather deforestation which leads to a reduction in evapotranspiration into the atmosphere, and a reduction in precipitation and its implicated for the gobal climate is discussed. It is indicated that a large scale clearing of tropical rainforests there would be a reduction in rainfall which would have global effects on climate and weather both in the tropical and extratropical regions.

Nobre, C. A.↗

The Telecommunications and Data Acquisition Progress Report 42-77

Activities in space communication, radio navigation, radio science, and ground-based astronomy are reported. Advanced systems for the Deep Space Network and its Ground-Communications Facility are discussed including station control and system technology. Network sustaining as well as data and information systems are covered. Studies of geodynamics, investigations of the microwave spectrum, and the search for extraterrestrial intelligence are reported.

Posner, E. C.↗

Crustal magnetization and temperature at depth beneath the Yilgarn block, Western Australia inferred from Magsat data

Variations in crustal magnetization along a seismic section across the Archean Yilgarn block of Western Australia inferred from Magsat data are interpreted as a subtle thermal effect arising from variations in depth to the Curie isotherm. The isotherm lies deep within the mantle of the eastern part of the province, but transects the crust-mantle transition and rises well into the crust on the western side. The model is consistent with heat flow variations along the section line. The mean crustal magnetization implied by the model is approximately 2 A/m. The temperature variation implied by the model is consistent with the hypothesis that the crust-mantle transition seen seismically corresponds to the mafic granulite-eclogite phase transition within a zone of igneous crustal underplating.

Mayhew, M. A.↗

Improved Estimates of Clear Sky Longwave Flux and Application to the Tropical Greenhouse Effect

The first objective of this investigation is to eliminate the clear-sky offset introduced by the scene-identification procedures developed for the Earth Radiation Budget Experiment (ERBE). Estimates of this systematic bias range from 10 to as high as 30 W/sq m. The initial version of the ScaRaB data is being processed with the original ERBE algorithm. Since the ERBE procedure for scene identification is based upon zonal flux averages, clear scenes with longwave emission well below the zonal mean value are mistakenly classified as cloudy. The erroneous classification is more frequent in regions with deep convection and enhanced mid- and upper-tropospheric humidity. We will develop scene identification parameters with zonal and/or time dependence to reduce or eliminate the bias in the clear- sky data. The modified scene identification procedure could be used for the ScaRaB-specific version of the Earth-radiation products. The second objective is to investigate changes in the clear-sky Outgoing Longwave Radiation (OLR) associated with decadal variations in the tropical and subtropical climate. There is considerable evidence for a shift in the climate state starting in approximately 1977. The shift is accompanied by higher SSTs in the equatorial Pacific, increased tropical convection, and higher values of atmospheric humidity. Other evidence indicates that the humidity in the tropical troposphere has been steadily increasing over the last 30 years. It is not known whether the atmospheric greenhouse effect has increased during this period in response to these changes in SST and precipitable water. We will investigate the decadal-scale fluctuations in the greenhouse effect using Nimbus-7, ERBE, and ScaRaB measurements spaning 1979 to the present. The data from the different satellites will be intercalibrated by comparison with model calculations based upon ship radiosonde observations. The fluxes calculated from the radiation model will also be used for validation of the ScaRaB fluxes.

Collins, W. D.↗

A Global Ocean Tide Model From TOPEX/POSEIDON Altimetry: GOT99.2

Goddard Ocean Tide model GOT99.2 is a new solution for the amplitudes and phases of the global oceanic tides, based on over six years of sea-surface height measurements by the TOPEX/POSEIDON satellite altimeter. Comparison with deep-ocean tide-gauge measurements show that this new tidal solution is an improvement over previous global models, with accuracies for the main semidiurnal lunar constituent M2 now below 1.5 cm (deep water only). The new solution benefits from use of prior hydrodynamic models, several in shallow and inland seas as well as the global finite-element model FES94.1. This report describes some of the data processing details involved in handling the altimetry, and it provides a comprehensive set of global cotidal charts of the resulting solutions. Various derived tidal charts are also provided, including tidal loading deformation charts, tidal gravimetric charts, and tidal current velocity (or transport) charts. Finally, low-degree spherical harmonic coefficients are computed by numerical quadrature and are tabulated for the major short-period tides; these are useful for a variety of geodetic and geophysical purposes, especially in combination with similar estimates from satellite laser ranging.

Ray, Richard D.↗

Deep Space Control Challenges of the New Millennium

The exploration of deep space presents a variety of significant control challenges. Long communication delays coupled with challenging new science objectives require high levels of system autonomy and increasingly demanding pointing and control capabilities. Historically, missions based on the use of a large single spacecraft have been successful and popular since the early days of NASA. However, these large spacecraft missions are currently being displaced by more frequent and more focused missions based on the use of smaller and less expensive spacecraft designs. This trend drives the need to design smart software and good algorithms which together with the miniaturization of control components will improve performance while replacing the heavier and more expensive hardware used in the past. NASA's future space exploration will also include mission types that have never been attempted before, posing significant challenges to the underlying control system. This includes controlled landing on small bodies (e.g., asteroids and comets), sample return missions (where samples are brought back from other planets), robotic exploration of planetary surfaces (e.g., intelligent rovers), high precision formation flying, and deep space optical interferometry, While the control of planetary spacecraft for traditional flyby and orbiter missions are based on well-understood methodologies, control approaches for many future missions will be fundamentally different. This paradigm shift will require completely new control system development approaches, system architectures, and much greater levels of system autonomy to meet expected performance in the presence of significant environmental disturbances, and plant uncertainties. This paper will trace the motivation for these changes and will layout the approach taken to meet the new challenges. Emerging missions will be used to explain and illustrate the need for these changes.

Bayard, David S.↗

Drought Indicators Based on Model Assimilated GRACE Terrestrial Water Storage Observations

The Gravity Recovery and Climate Experiment (GRACE) twin satellites observe time variations in Earth's gravity field which yield valuable information about changes in terrestrial water storage (TWS). GRACE is characterized by low spatial (greater than 150,000 square kilometers) and temporal (greater than 10 day) resolution but has the unique ability to sense water stored at all levels (including groundwater) systematically and continuously. The GRACE Data Assimilation System (GRACE-DAS), based on the Catchment Land Surface Model (CLSM) enhances the value of the GRACE water storage data by enabling spatial and temporal downscaling and vertical decomposition into moisture 39 components (i.e. groundwater, soil moisture, snow), which individually are more useful for scientific applications. In this study, GRACE-DAS was applied to North America and GRACE-based drought indicators were developed as part of a larger effort that investigates the possibility of more comprehensive and objective identification of drought conditions by integrating spatially, temporally and vertically disaggregated GRACE data into the U.S. and North American Drought Monitors. Previously, the Drought Monitors lacked objective information on deep soil moisture and groundwater conditions, which are useful indicators of drought. Extensive datasets of groundwater storage from USGS monitoring wells and soil moisture from the Soil Climate Analysis Network (SCAN) were used to assess improvements in the hydrological modeling skill resulting from the assimilation of GRACE TWS data. The results point toward modest, but statistically significant, improvements in the hydrological modeling skill across major parts of the United States, highlighting the potential value of GRACE assimilated water storage field for improving drought detection.

Houborg, Rasmus↗

Reduction of Non-uniform Beam Filling Effects by Vertical Decorrelation: Theory and Simulations

Algorithms for estimating precipitation rates from spaceborne radar observations of apparent radar reflectivity depend on attenuation correction procedures. The algorithm suite for the Ku-band precipitation radar aboard the Tropical Rainfall Measuring Mission satellite is one such example. The well-known problem of nonuniform beam filling is a source of error in the estimates, especially in regions where intense deep convection occurs. The error is caused by unresolved horizontal variability in precipitation characteristics such as specific attenuation, rain rate, and effective reflectivity factor. This paper proposes the use of vertical decorrelation for correcting the nonuniform beam filling error developed under the assumption of a perfect vertical correlation. Empirical tests conducted using ground-based radar observations in the current simulation study show that decorrelation effects are evident in tilted convective cells. However, the problem of obtaining reasonable estimates of a governing parameter from the satellite data remains unresolved.

radar reflectivity↗

Extreme Temperature Performance of Automotive-Grade Small Signal Bipolar Junction Transistors

Electronics designed for space exploration missions must display efficient and reliable operation under extreme temperature conditions. For example, lunar outposts, Mars rovers and landers, James Webb Space Telescope, Europa orbiter, and deep space probes represent examples of missions where extreme temperatures and thermal cycling are encountered. Switching transistors, small signal as well as power level devices, are widely used in electronic controllers, data instrumentation, and power management and distribution systems. Little is known, however, about their performance in extreme temperature environments beyond their specified operating range; in particular under cryogenic conditions. This report summarizes preliminary results obtained on the evaluation of commercial-off-the-shelf (COTS) automotive-grade NPN small signal transistors over a wide temperature range and thermal cycling. The investigations were carried out to establish a baseline on functionality of these transistors and to determine suitability for use outside their recommended temperature limits.

Thermal Cycling↗

Architectural and Operational Considerations Emerging from Hybrid RF-Optical Network Loading Simulations

A technology demonstration of free space optical communication at interplanetary distances is planned via one or more future NASA deep-space missions. Such demonstrations will “pave the way” for operational use of optical communications on future robotic/potential Human missions. Hence, the Deep Space Network architecture will need to evolve. Preliminary attempts to model the anticipated future mission set and simulate how well it loads onto assumed architectures with combinations of RF and optical apertures have been evaluated. This paper discusses the results of preliminary loading simulations for hybrid RF-optical network architectures and highlights key mission and ground infrastructure considerations that emerge.

loading simulations↗

A Quantitative Analysis On the Use Of Supervised Machine Learning in Earth Science

Several recent papers have investigated different challenges in applying machine learning (ML) techniques to Earth science problems. The challenges listed range from interpretability of the results to computational demand to data issues. In this paper, we focus on specific challenges listed in the review papers that are centered around training data, as the size of training data is important in applying deep learning (DL) techniques. We are in the process of conducting a literature survey to better understand these challenges as well as to understand any trends. As part of this survey, our review has encompassed Earth science papers from AGU, AMS, IEEE and SPIE journals covering the last ten years and focused on papers that utilize supervised ML techniques.

Katrina S Virts↗

Modification of Caloris ejecta blocks by long-lived mass-wasting: A volatile-driven process?

The Caloris basin is the largest well-preserved impact basin on Mercury. As such, Caloris ejecta afford us an opportunity to study material from Mercury's deep interior with remote sensing. We have made observations of the geomorphology, colour, distribution, and flank slopes of the circum-Caloris knobs. Our observations suggest that these circum-Caloris knobs are modified ejecta blocks from the Caloris impact. High-resolution MESSENGER images show that knobs are conical and relatively uncratered compared with the surrounding plains, which implies the knobs have undergone resurfacing. We have observed material that has sloughed off knobs superposing impact craters that demonstrably postdate the Caloris impact, which requires some knob modification to have been more recent. We have observed hollows, depressions in Mercury's surface generally believed to have been caused by volatile-loss, on and closely associated with several knobs, which indicates that many knobs contain volatile material and that knob modification could extend into Mercury's recent past. Our measurements show that knob flanks typically have slopes of ∼21°, which is steep for a mound of unconsolidated material that was originally emplaced ∼3.8 Ga. The conical shape of knobs, their steep slopes, the dearth of superposed craters on knobs, and knob superposition relationships with other landforms suggest that Caloris ejecta blocks of arbitrary original shape were modified into their present shapes by long-lived mass-wasting. Mass-wasting must have dominated over impact gardening, which would have produced domal morphologies only. We suggest that mass-wasting was probably driven by volatile-loss, in a manner analogous to terrestrial landforms called ‘molards’. If the circum-Caloris knobs are analogous to molards, then they represent a landform and a process hitherto undocumented on Mercury, with implications for the volatile content of the planet's interior. These knobs therefore are prime targets for BepiColombo, which could search for fresh failures and volatile exposures in the knobs.

Mercury; Caloris basin; impact ejecta; volatiles; ↗