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At least 541 records · Page 30

“Beam à la carte”: Laser heater shaping for attosecond pulses in a multiplexed x-ray free-electron laser

Electron beam shaping allows the control of the temporal properties of x-ray free-electron laser pulses from femtosecond to attosecond timescales. Here, we demonstrate the use of a laser heater to shape electron bunches and enable the generation of attosecond x-ray pulses. We demonstrate that this method can be applied in a selective way, shaping a targeted subset of bunches while leaving the remaining bunches unchanged. This experiment enables the delivery of shaped x-ray pulses to multiple undulator beamlines, with pulse properties tailored to specialized scientific applications.

43 PARTICLE ACCELERATORS↗

Arrayed in vivo barcoding for multiplexed sequence verification of plasmid DNA and demultiplexing of pooled libraries

Sequence verification of plasmid DNA is critical for many cloning and molecular biology workflows. To leverage high-throughput sequencing, several methods have been developed that add a unique DNA barcode to individual samples prior to pooling and sequencing. However, these methods require an individual plasmid extraction and/or in vitro barcoding reaction for each sample processed, limiting throughput and adding cost. Here, we develop an arrayed in vivo plasmid barcoding platform that enables pooled plasmid extraction and library preparation for Oxford Nanopore sequencing. This method has a high accuracy and recovery rate, and greatly increases throughput and reduces cost relative to other plasmid barcoding methods or Sanger sequencing. We use in vivo barcoding to sequence verify >45 000 plasmids and show that the method can be used to transform error-containing dispersed plasmid pools into sequence-perfect arrays or well-balanced pools. In vivo barcoding does not require any specialized equipment beyond a low-overhead Oxford Nanopore sequencer, enabling most labs to flexibly process hundreds to thousands of plasmids in parallel.

59 BASIC BIOLOGICAL SCIENCES↗

A Frequency Domain Multiplexing Technique for Multi-Channel Detector Instrumentation

Radiation detection often requires several detectors to be employed at once. Normally, each detector outputs a signal that must be transported, digitized, and stored. One common format for these signals is a finite, aperiodic pulse. Since the detector and data acquisition system are not necessarily physically close, transporting signals from several detectors to a central data acquisition system often requires the use of many cables. The technique presented here allows for the combination and later recovery of a number of analog detector signals using a single cable, by shifting their content to narrow bands in the frequency domain and summing them together into a single channel for transmission. The output is a linear combination of decaying sinusoids, whose peak frequencies and bandwidths in the frequency domain are controlled by inductor, resistor, and capacitor values in the circuit. By separating the outputs by several megahertz, and lowering the bandwidth to about 1 MHz, we can isolate each signal. The timing of the original input can be recovered by taking the inverse Fourier transform of the isolated peak and identifying the start time of the resulting single decaying sinusoid. The energy of the input signal is proportional to the square root of the integral of the corresponding power spectrum peak. We show that energy spectra can be reconstructed with minimal additions to energy resolution. We also show that coincidence timing measurements can be performed with an uncertainty smaller than 2 ns. The result is an N to 1 reduction in cabling, with obvious cost and complexity gains. Since most of the signal content exists only in a band around each peak frequency, noise outside of these bands does not impact the performance.

Belling, Samuel↗

High-sensitivity high-throughput chip based biosensor array for multiplexed detection of heavy metals

Heavy metal ions released into the environment from industrial processes lead to various health hazards. We propose an on-chip label-free detection approach that allows high-sensitivity and high-throughput detection of heavy metals. The sensing device consists of 2-dimensional photonic crystal microcavities that are combined by multimode interferometer to form a sensor array. Here, we experimentally demonstrate the detection of cadmium-chelate conjugate with concentration as low as 5 parts-per-billion (ppb).

42 ENGINEERING↗