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At least 541 records · Page 30
All sourcesNASA NTRSDOE OSTI
NASA NTRS2017

Coupling Between Metabolism and Compartmentalization: Vesicle Growth in the Presence of Dipeptides

A fundamental unresolved question in studies on the origin of life is: how different, ubiquitous protocellular functions begun to work in concert setting the stage for Darwinian evolution of nascent life? From this perspective, of particular significance is coupling between growth of protocellular compartments and the encapsulated, primordial metabolism, which is one of the focal topics of the current ISSOL meeting. Specifically, growth and division of cells facilitated by the products of a metabolic reaction would confer an evolutionary advantage on protocells encapsulating this reaction, as their population would increase at the expense of other protocells. Along these lines, Adamala and Szostak have recently demonstrated that a dipeptide captured inside fatty acid vesicles catalyzes the formation of other dipeptides from activated monomers. Some of the newly synthesized dipeptides, in turn, are capable to promote competitive growth of vesicles in the presence of fatty acid micelles. As vesicles become larger, they adapt filamentous shape, which has been shown to promote their division. On the basis of computer simulations, we provide a molecularly detailed explanation of this process and draw conclusions about its generality.

Wei, C.↗
NASA NTRS2019

Verification and Validation of a Three-Dimensional Composite Impact Model with Tabulated Input

A material model which incorporates several key capabilities which have been identified by the aerospace community as lacking in the composite impact models currently available in the commercial transient dynamic finite element code LS-DYNA® has been developed. The material model utilizes experimentally based tabulated input to define the evolution of plasticity and damage as opposed to specifying discrete input parameters (such as modulus and strength. The plasticity portion of the orthotropic, three-dimensional, macroscopic composite constitutive model is based on an extension of the Tsai-Wu composite failure model into a generalized yield function with a non-associative flow rule. The capability to account for the rate and temperature dependent deformation response of composites has also been incorporated into the material model. For the damage model, a strain equivalent formulation is utilized to allow for the uncoupling of the deformation and damage analyses. In the damage model, a diagonal damage tensor is defined to account for the directionally dependent variation of damage. However, the terms in the damage matrix are semi-coupled such that the damage in a particular coordinate direction is a function of the stresses and plastic strains in all of the coordinate directions. For the failure model, a tabulated approach is utilized in which a stress or strain based invariant is defined as a function of the location of the current stress state in stress space to define the initiation of failure, which allows an arbitrarily shaped failure surface to be defined. A systematic series of validation and verification studies, at a variety of length scales ranging from single element simulations to simulations of a flat panel impact test, have been performed to fully exercise and evaluate the capabilities of the developed model.

Impact↗
NASA NTRS1998

Extracellular calcium (Ca2+o)-sensing receptor in a mouse monocyte-macrophage cell line (J774): potential mediator of the actions of Ca2+o on the function of J774 cells

The calcium-sensing receptor (CaR) is a G protein-coupled receptor that plays key roles in extracellular calcium ion (Ca2+o) homeostasis in parathyroid gland and kidney. Macrophage-like mononuclear cells appear at sites of osteoclastic bone resorption during bone remodeling and may play a role in the "reversal" phase following osteoclastic resorption and preceding bone formation. Bone resorption produces substantial local increases in Ca2+o that could provide a signal for bone marrow mononuclear cells in the vicinity, leading us to investigate whether such mononuclear cells express the CaR. In this study, we used the mouse J774 cell line, which exhibits a pure monocyte-macrophage phenotype. Both immunocytochemistry and Western blot analysis, using polyclonal antisera specific for the CaR, detected CaR protein in J774 cells. The use of reverse transcriptase-polymerase chain reaction with CaR-specific primers, including a set of intron-spanning primers, followed by nucleotide sequencing of the amplified products, also identified CaR transcripts in J774 cells. Exposure of J774 cells to high Ca2+o (2.8 mM or more) or the polycationic CaR agonist, neomycin (100 microM), stimulated both chemotaxis and DNA synthesis in J774 cells. Therefore, taken together, our data strongly suggest that the monocyte-macrophage cell line, J774, possesses both CaR protein and mRNA very similar, if not identical, to those in parathyroid and kidney.

NASA Discipline Musculoskeletal↗
NASA NTRS1998

Extracellular calcium (Ca2+(o))-sensing receptor in a murine bone marrow-derived stromal cell line (ST2): potential mediator of the actions of Ca2+(o) on the function of ST2 cells

The calcium-sensing receptor (CaR) is a G protein-coupled receptor that plays key roles in extracellular calcium ion (Ca2+(o)) homeostasis by mediating the actions of Ca2+(o) on parathyroid gland and kidney. Bone marrow stromal cells support the formation of osteoclasts from their progenitors as well as the growth of hematopoietic stem cells by secreting humoral factors and through cell to cell contact. Stromal cells also have the capacity to differentiate into bone-forming osteoblasts. Bone resorption by osteoclasts probably produces substantial local increases in Ca2+(o) that could provide a signal for stromal cells in the immediate vicinity, leading us to determine whether such stromal cells express the CaR. In this study, we used the murine bone marrow-derived, stromal cell line, ST2. Both immunocytochemistry and Western blot analysis, using an antiserum specific for the CaR, detected CaR protein in ST2 cells. We also identified CaR transcripts in ST2 cells by Northern analysis using a CaR-specific probe and by RT-PCR with CaR-specific primers, followed by nucleotide sequencing of the amplified products. Exposure of ST2 cells to high Ca2+(o) (4.8 mM) or to the polycationic CaR agonists, neomycin (300 microM) or gadolinium (100 microM), stimulated both chemotaxis and DNA synthesis in ST2 cells. Therefore, taken together, our data strongly suggest that the bone marrow-derived stromal cell line, ST2, possesses both CaR protein and messenger RNA that are very similar if not identical to those in parathyroid and kidney. Furthermore, as ST2 cells have the potential to differentiate into osteoblasts, the CaR in stromal cells could participate in bone turnover by stimulating the proliferation and migration of such cells to sites of bone resorption as a result of local, osteoclast-mediated release of Ca2+(o) and, thereafter, initiating bone formation after their differentiation into osteoblasts.

NASA Discipline Musculoskeletal↗
NASA NTRS1993

Quantum wormholes and harmonic oscillators

The quantum state of a wormhole can be represented by a path integral over all asymptotically Euclidean four-geometries and all matter fields which have prescribed values, the arguments of the wave function, on a three-surface which divides the space time manifold into two disconnected parts. Minisuperspace models which consist of a homogeneous massless scalar field coupled to a Friedmann-Robertson-Walker space time are considered. Once the path integral over the lapse function is performed, the requirement that the space time be asymptotically Euclidean can be accomplished by fixing the asymptotic gravitational momentum in the remaining path integral. It is argued that there does not exist any wave function which corresponds to asymptotic field configurations such that the effective gravitational constant is negative in the asymptotic region. Then, the wormhole wave functions can be written as linear combinations of harmonic oscillator wave functions.

Garay, Luis J.↗
NASA NTRS2016

Incorporation of Damage and Failure into an Orthotropic Elasto-Plastic Three-Dimensional Model with Tabulated Input Suitable for Use in Composite Impact Problems

A material model which incorporates several key capabilities which have been identified by the aerospace community as lacking in the composite impact models currently available in LS-DYNA(Registered Trademark) is under development. In particular, the material model, which is being implemented as MAT 213 into a tailored version of LS-DYNA being jointly developed by the FAA and NASA, incorporates both plasticity and damage within the material model, utilizes experimentally based tabulated input to define the evolution of plasticity and damage as opposed to specifying discrete input parameters (such as modulus and strength), and is able to analyze the response of composites composed with a variety of fiber architectures. The plasticity portion of the orthotropic, three-dimensional, macroscopic composite constitutive model is based on an extension of the Tsai-Wu composite failure model into a generalized yield function with a non-associative flow rule. The capability to account for the rate and temperature dependent deformation response of composites has also been incorporated into the material model. For the damage model, a strain equivalent formulation is utilized to allow for the uncoupling of the deformation and damage analyses. In the damage model, a diagonal damage tensor is defined to account for the directionally dependent variation of damage. However, in composites it has been found that loading in one direction can lead to damage in multiple coordinate directions. To account for this phenomena, the terms in the damage matrix are semi-coupled such that the damage in a particular coordinate direction is a function of the stresses and plastic strains in all of the coordinate directions. The onset of material failure, and thus element deletion, is being developed to be a function of the stresses and plastic strains in the various coordinate directions. Systematic procedures are being developed to generate the required input parameters based on the results of experimental tests.

Finite Element Method↗
NASA NTRS1978

Navier-Stokes calculations with a coupled strongly implicit method. Part 2: Spline solutions

A coupled strongly implicit method is combined with a deferred-corrector spline solver for the vorticity-stream function form of the Navier-Stokes equation. Solutions for cavity, channel and cylinder flows are obtained with the fourth-order spline 4 procedure. The strongly coupled spline corrector method converges as rapidly as the finite difference calculations and also allows for arbitrary large time increments for the Reynolds numbers considered. In some cases fourth-order smoothing or filtering is required in order to suppress high frequency oscillations.

Rubin, S. G.↗
NASA NTRS1979

Data pattern sensitivity in tracking performance of an AC coupled Costas loop with hard-limited in-phase channel

This paper is concerned with data pattern sensitivity in carrier tracking performance of an AC coupled Costas loop with a suppressed BPSK signal. The signal amplitude suppression factor is derived as a function of data 'asymmetry ratio' - the ratio of '1's to the total number of bits in a period of a periodic signal. For an asymmetric pattern, the effect of AC coupling is noticeable whereas there is almost no effect for symmetric square wave. The tracking performance with an asymmetric pattern is worse than that with a symmetric pattern. However, it is also shown that as expected, the tracking performance of a DC coupled loop with an asymmetric pattern is better than that with a symmetric pattern.

Park, Y. H.↗
NASA NTRS1974

The effect of cyclic feathering motions on dynamic rotor loads

The dynamic loads of a helicopter rotor in forward flight are influenced significantly by the geometric pitch angles between the structural axes of the hub and blade sections and the plane of rotation. The analytical study presented includes elastic coupling between inplane and out-of-plane deflections as a function of geometric pitch between the plane of rotation and the principal axes of inertia of each blade. The numerical evaluation is based on a transient analysis using lumped masses and elastic substructure techniques. A comparison of cases with and without cyclic feathering motion shows the effect on computed dynamic rotor loads.

Harvey, K. W.↗
NASA NTRS1975

Effects of ionizing radiation on charge-coupled imagers

The effects of ionizing radiation on three different charge coupled imagers have been investigated. Device performance was evaluated as a function of total gamma ray dose. The principal failure mechanisms have been identified for each particular device structure. The clock and bias voltages required for high total dose operation of the devices are presented.

Killiany, J. M.↗
NASA NTRS1978

An experiment to verify that the weak interactions satisfy the strong equivalence principle

The construction of a clock based on the beta decay process is proposed to test for any violations by the weak interaction of the strong equivalence principle bu determining whether the weak interaction coupling constant beta is spatially constant or whether it is a function of gravitational potential (U). The clock can be constructed by simply counting the beta disintegrations of some suitable source. The total number of counts are to be taken a measure of elapsed time. The accuracy of the clock is limited by the statistical fluctuations in the number of counts, N, which is equal to the square root of N. Increasing N gives a corresponding increase in accuracy. A source based on the electron capture process can be used so as to avoid low energy electron discrimination problems. Solid state and gaseous detectors are being considered. While the accuracy of this type of beta decay clock is much less than clocks based on the electromagnetic interaction, there is a corresponding lack of knowledge of the behavior of beta as a function of gravitational potential. No predictions from nonmetric theories as to variations in beta are available as yet, but they may occur at the U/sg C level.

Eby, P. B.↗
NASA NTRS1986

Transonic viscous and inviscid solutions using a composite velocity procedure

Solutions for transonic viscous and inviscid flows using a composite velocity procedure are presented. The velocity components of the compressible Navier-Stokes equations are written in terms of a multiplicative composite consisting of a viscous or rotational velocity and an inviscid, irrotational, potential like function. For viscous flows, the governing equations are solved using a coupled strongly implicit procedure and the outer inviscid flow corresponds to an irrotational, potential flow. Further investigation of the inviscid flow solution is made so that the correct rotational flow solution may be obtained for the transonic case. The Euler equations are solved for the inviscid flow calculations. Solutions to the full potential equation are recovered unless the axial momentum equation is written in full conservation form.

Gordnier, R. E.↗
NASA NTRS1988

Aeroacoustics of advanced STOVL aircraft plumes

This paper summarizes a basic and well-controlled experimental study involving flow visualization and noise measurements to define the acoustic and flow fields of single plumes impinging on a simulated ground plane. The flow visualization was made by strobing a laser light source at the discrete frequencies generated by the impingement of the jets and measured by a nearfield microphone. This enabled visualization of instability waves generated by the interaction between the plumes and the sound generated during impingement, and also by dynamic coupling between the two plumes. These data were acquired as a function of distance between the ground and the nozzle exit. Nearfield acoustic data were acquired simultaneously. Data for nozzle diameters of 0.265 in. and 0.4 in. are described. For selected nozzles, effects of exit boundary layer characteristics and nozzle protrusion through a simulated aircraft body are also presented.

Ahuja, K. K.↗
NASA NTRS1989

The thermal catastrophe model of substorms

Equations are presented for the physical mechanisms involved in the resonant absorption of Alfven waves in the plasma sheet boundary layer. It is shown that energy absorbed by the plasma-sheet particles is a function of the central plasma sheet temperature. The heating curve, when coupled with convective transport, yielded an equation of state for the steady state plasma sheet, whose solution has the form of a mathematical catastrophe. The master equation includes dynamic terms describing the transition across the thermal catastrophe, making it possible to evaluate the time scale for the catastrophe to occur.

Goertz, C. K.↗
NASA NTRS1992

Crustal deformation along the San Andreas, California

The goal is to achieve a better understanding of the regional and local deformation and crustal straining processes in western North America, particularly the effects of the San Andreas and nearby faults on the spatial and temporal crustal deformation behavior. Construction of theoretical models based on the mechanics of coupled elastic plate, viscoelastic foundation and large scale crack mechanics provide a rational basis for the interpretation of seismic and aseismic anomalies and expedite efforts in forecasting the stability of plate boundary deformation. Special focus is placed on the three dimensional time dependent surface deformation due to localized slippage in a elastic layer coupled to a visco-elastic substrate. The numerical analysis is based on a 3-D boundary element technique. Extension to visco-elastic coupling demands the derivation of 3-D time dependent Green's function. This method was applied to analyze the viscoelastic surface displacements due to a dislocated embedded patch. Surface uplift as a function of time and position are obtained. Comparisons between surface uplift for long and short dislocated patches are made.

Li, Victor C.↗
NASA NTRS1994

Electron collection by a highly positive satellite in the ionosphere: Test aprticle simulation

Collection of electrons by a satellite at a relatively high potential in low Earth orbit, including the effects of the satellite's orbital motion, remains a poorly understood phenomenon. Using a test particle simulation in which charged particle motion is tracked in prescribed electric fields, we calculate here the current collection and the nature of the energy distribution function of the electrons collected by the satellite, including the effects of the satellite orbital motion. Calculations of the collected current without the orbital motion show an excellent agreement with the current from the Parker-Murphy model, but with the orbital motion of the satellite the current collection is enhanced, the degree of enhancement depending on the size of the satellite sheath extending along the magnetic field line. In the latter case, the flow of electrons around the satellite shows some interesting behaviors including azimuthal E x B drift around the satellite, axial trapping along the magnetic field, and formation of field-aligned flow of electrons in the wake region. The total energy of the collected electrons is approximately equal e phi(sub 0), where phi(sub 0) is the satellite potential, but the partition of the energy into components parallel (W(sub parallel)) and perpendicular (W(sub perpendicular)) to the magnetic field shows interesting features. For the magnetic field along the polar axis Z, the energy distributions near the poles are perfectly field aligned, that is, the parallel energy (W(sub parallel) = e phi(sub 0). The perpendicular component (W(sub perpendicular)) progressively increases toward the 'equator' of the spherical satellite. For a sufficiently large bias potential phi(sub 0), the equatorial energy distributions f(W(sub parallel)) and f(W(sub perpendicular)) show fine structures having multiple peaks. These features of the distribution functions result from the chaotic motion of electrons and the associated coupling between the electron motions parallel and perpendicular to the magnetic field in strongly nonuniform electric fields near the satellite.

Singh, Nagendra↗