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At least 541 records · Page 30
All sourcesNASA NTRSDOE OSTI
DOE OSTI2024

Analysis of twelve genomes of the bacterium Kerstersia gyiorum from brown-throated sloths ( Bradypus variegatus ), the first from a non-human host

Kerstersia gyiorum is a Gram-negative bacterium found in various animals, including humans, where it has been associated with various infections. Knowledge of the basic biology of K. gyiorum is essential to understand the evolutionary strategies of niche adaptation and how this organism contributes to infectious diseases; however, genomic data about K. gyiorum is very limited, especially from non-human hosts. In this work, we sequenced 12 K. gyiorum genomes isolated from healthy free-living brown-throated sloths (Bradypus variegatus) in the Parque Estadual das Fontes do Ipiranga (São Paulo, Brazil), and compared them with genomes from isolates of human origin, in order to gain insights into genomic diversity, phylogeny, and host specialization of this species. Phylogenetic analysis revealed that these K. gyiorum strains are structured according to host. Despite the fact that sloth isolates were sampled from a single geographic location, the intra-sloth K. gyiorum diversity was divided into three clusters, with differences of more than 1,000 single nucleotide polymorphisms between them, suggesting the circulation of various K. gyiorum lineages in sloths. Genes involved in mobilome and defense mechanisms against mobile genetic elements were the main source of gene content variation between isolates from different hosts. Sloth-specific K. gyiorum genome features include an IncN2 plasmid, a phage sequence, and a CRISPR-Cas system. The broad diversity of defense elements in K. gyiorum (14 systems) may prevent further mobile element flow and explain the low amount of mobile genetic elements in K. gyiorum genomes. Gene content variation may be important for the adaptation of K. gyiorum to different host niches. This study furthers our understanding of diversity, host adaptation, and evolution of K. gyiorum, by presenting and analyzing the first genomes of non-human isolates.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2023

Saccharomycopsis sp. strain UFMG-CM-Y6632 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence

Three yeast isolates were obtained from soil and rotting wood samples collected in an Amazonian rainforest biome in Brazil. Comparison of the intergenic spacer 5.8S region and the D1/D2 domains of the large subunit rRNA gene showed that the isolates represent a novel species of the genus Saccharomycopsis. A tree inferred from the D1/D2 sequences placed the novel species near a subclade containing Saccharomycopsis lassenensis, Saccharomycopsis fermentans, Saccharomycopsis javanensis, Saccharomycopsis babjevae, Saccharomycopsis schoenii and Saccharomycopsis oosterbeekiorum, but with low bootstrap support. In terms of sequence divergence, the novel species had the highest identity in the D1/D2 domains with Saccharomycopsis capsularis, from which it differed by 36 substitutions. In contrast, a phylogenomic analysis based on 1061 single-copy orthologs for a smaller set of Saccharomycopsis species whose whole genome sequences are available indicated that the novel species represented by strain UFMG-CM-Y6991 is phylogenetically closer to Saccharomycopsis fodiens and Saccharomycopsis sp. TF2021a (=Saccharomycopsis phalluae). The novel yeast is homothallic and produces asci with one spheroidal ascospore with an equatorial or subequatorial ledge. The name Saccharomycopsis praedatoria sp. nov. is proposed to accommodate the novel species. The holotype of Saccharomycopsis praedatoria is CBS 16589(T). The MycoBank number is MB849369. S. praedatoria was able to kill cells of Saccharomyces cerevisiae by means of penetration with infection pegs, a trait common to most species of Saccharomycopsis.

Amazonian Forest↗
DOE OSTI2022

Saccharomycopsis sp. isolate UFMG-CM-Y6632 large subunit ribosomal RNA gene, partial sequence

Three yeast isolates were obtained from soil and rotting wood samples collected in an Amazonian rainforest biome in Brazil. Comparison of the intergenic spacer 5.8S region and the D1/D2 domains of the large subunit rRNA gene showed that the isolates represent a novel species of the genus Saccharomycopsis. A tree inferred from the D1/D2 sequences placed the novel species near a subclade containing Saccharomycopsis lassenensis, Saccharomycopsis fermentans, Saccharomycopsis javanensis, Saccharomycopsis babjevae, Saccharomycopsis schoenii and Saccharomycopsis oosterbeekiorum, but with low bootstrap support. In terms of sequence divergence, the novel species had the highest identity in the D1/D2 domains with Saccharomycopsis capsularis, from which it differed by 36 substitutions. In contrast, a phylogenomic analysis based on 1061 single-copy orthologs for a smaller set of Saccharomycopsis species whose whole genome sequences are available indicated that the novel species represented by strain UFMG-CM-Y6991 is phylogenetically closer to Saccharomycopsis fodiens and Saccharomycopsis sp. TF2021a (=Saccharomycopsis phalluae). The novel yeast is homothallic and produces asci with one spheroidal ascospore with an equatorial or subequatorial ledge. The name Saccharomycopsis praedatoria sp. nov. is proposed to accommodate the novel species. The holotype of Saccharomycopsis praedatoria is CBS 16589(T). The MycoBank number is MB849369. S. praedatoria was able to kill cells of Saccharomyces cerevisiae by means of penetration with infection pegs, a trait common to most species of Saccharomycopsis.

Amazonian Forest↗
DOE OSTI2023

Saccharomycopsis sp. KB-2023a isolate UFMG-CM-Y6991, whole genome shotgun sequencing project

Three yeast isolates were obtained from soil and rotting wood samples collected in an Amazonian rainforest biome in Brazil. Comparison of the intergenic spacer 5.8S region and the D1/D2 domains of the large subunit rRNA gene showed that the isolates represent a novel species of the genus Saccharomycopsis. A tree inferred from the D1/D2 sequences placed the novel species near a subclade containing Saccharomycopsis lassenensis, Saccharomycopsis fermentans, Saccharomycopsis javanensis, Saccharomycopsis babjevae, Saccharomycopsis schoenii and Saccharomycopsis oosterbeekiorum, but with low bootstrap support. In terms of sequence divergence, the novel species had the highest identity in the D1/D2 domains with Saccharomycopsis capsularis, from which it differed by 36 substitutions. In contrast, a phylogenomic analysis based on 1061 single-copy orthologs for a smaller set of Saccharomycopsis species whose whole genome sequences are available indicated that the novel species represented by strain UFMG-CM-Y6991 is phylogenetically closer to Saccharomycopsis fodiens and Saccharomycopsis sp. TF2021a (=Saccharomycopsis phalluae). The novel yeast is homothallic and produces asci with one spheroidal ascospore with an equatorial or subequatorial ledge. The name Saccharomycopsis praedatoria sp. nov. is proposed to accommodate the novel species. The holotype of Saccharomycopsis praedatoria is CBS 16589(T). The MycoBank number is MB849369. S. praedatoria was able to kill cells of Saccharomyces cerevisiae by means of penetration with infection pegs, a trait common to most species of Saccharomycopsis.

Amazonian Forest↗
DOE OSTI2024

Pichia sp. KDB-2024a strain:UFMG-CM-Y531 Genome sequencing

Pichia senei was isolated from necrotic cactus tissues of Cereus saddianus and Micranthocereus dolichospermaticus, and from water tank (phytotelmata) of the bromeliad species Bromelia karatas, collected in the state of Tocantins, Brazil.

bromeliads↗
NASA NTRS1972

EXAMETNET: The first five years, 1966 - 1970

An assessment of the first five years of operation of the Experimental InterAmerican Meterological Rocket Network is presented. The formation of the network, and international and national relations are described. The technology and data procedures and techniques are reported, and their improvements and the research accomplished as a result of the cooperative effort among Argentina, Brazil, and the United States are discussed.

Source record↗
NASA NTRS1971

The spectra of ten galactic X-ray sources in the southern sky

Data on ten galactic X-ray sources were obtained during a rocket flight from Brazil in June 1969. Detailed spectra of these sources have been compared with bremsstrahlung, black body, and power law models, each including interstellar absorption. Six of the sources were fitted well by one or more of these models. In only one case were the data sufficient to distinguish the best model. Three of the sources were not fitted by any of the models, which suggests that more complex emission mechanisms are applicable. A comparison of our results with those of previous investigations provides evidence that five of the sources vary in intensity by a factor of 2 or more, and that three have variable spectra. New or substantially improved positions have been derived for four of the sources observed.

Cruddace, R.↗
NASA NTRS1972

The spectra of ten galactic X-ray sources in the southern sky.

Data on 10 galactic X-ray sources located between 320 and 20 deg galactic longitude were obtained during a rocket flight from Brazil in June 1969. Detailed spectra of these sources have been compared with bremsstrahlung, blackbody, and power-law models, each including interstellar absorption. Six of the sources were fitted well by one or more of these models. In only one case were the data sufficient to distinguish the best model. Three of the sources were not fitted by any of the models, which suggests that more complex emission mechanisms are applicable. A comparison of our results with those of previous investigations provides evidence that five of the sources vary in intensity by a factor of two or more, and that three have variable spectra.

Cruddace, R.↗
NASA NTRS1973

Conjugate photoelectron excitation of O I 4368 airglow emission.

Conjugate photoelectron fluxes are shown to be mainly responsible for the excitation of O I 4368 radiation observed with a grille spectrometer located at Langmuir Laboratory, New Mexico (L equals 1.7). On the basis of observations of O I 7774 and 4368 tropical emissions at Agulhas, Negras, Brazil (L equals 1.1), the contribution of radiative recombination to the New Mexico intensity is shown to be small. Conjugate photoelectron excitation at the Brazilian site has not been observed.

Christensen, A. B.↗
NASA NTRS1973

Satellite-tracking and earth-dynamics research programs

The following activities in Smithsonian Astrophysical Observatory's (SAO) earth-dynamics programs are covered: (1) satellite-tracking network operations; (2) satellite geodesy and geophysics programs; (3) atmospheric research. Approximately 46,000 successful range measurements were acquired by the SAO laser stations in Peru, South Africa, Brazil, and Arizona. The Peole satellite-tracking campaign conducted in conjunction with the Centre National d'Etudes Spatiales was completed in August 1973. The SAO network obtained 4482 validated returns of 310 arcs of Peole. These data are of particular value for obtaining more accurate gravity-field and zonal-harmonics coefficients.

Weiffenbach, G. C.↗
NASA NTRS1974

Observations and calculations of atmospheric helium 10830 emission

Observations and computer model calculations of helium excitation and transport were carried out. The following conclusions can be drawn: (1) the dawn helium density in the thermosphere is greater than the dusk helium density by 50 per cent or more, in contrast to the static diffusion models but in agreement with the dynamic models; (2) the computed He 10830 intensities are inconsistent with the observations when use of the new Penning ionization cross section for loss of atomic oxygen is used; (3) conjugate photoelectron precipitation is responsible for excitation of atmospheric helium at middle latitudes, but it is not evident in the observations at the equator or Brazil (L equals 1.1); (4) the computations predict greater conjugate effects than observed, leading to the possibility of strong interhemispheric absorption.

Christensen, A. B.↗
NASA NTRS1975

Design data collection with Skylab microwave radiometer-scatterometer S-193, volume 2

The author has identified the following significant results. Skylab S-193 radiometer/scatterometer produced terrain responses with various polarizations and observation angles for cells of 100 to 400 sq km area. Classification of the observations into natural categories was achieved by K-means and spatial clustering algorithms. Microwave data acquired over the Great Salt Lake Desert area by sensors aboard Skylab and Nimbus 5 indicate that the microwave emission and backscatter were strongly influenced by contributions from subsurface layers of sediment saturated with brine. Correlations were noted between microwave backscatter response at approximately 33 deg from scatterometer (operating at 13.9 GHz) and the configuration of ground targets in Brazil as discerned from coarse scale maps. With limited, available ground truth, these correlations were sufficient to permit the production of image-like displays which bear a marked resemblance to known terrain features in several instances.

Moore, R. K.↗
NASA NTRS1975

Design Data Collection with Skylab Microwave Radiometer-Scatterometer S-193, Volume 1

The author has identified the following significant results. Observations with S-193 have provided radar design information for systems to be flown on spacecraft, but only at 13.9 GHz and for land areas over the United States and Brazil plus a few other areas of the world for which this kind of analysis was not made. Observations only extended out to about 50 deg angle of incidence. The value of a sensor with such a gross resolution for most overland resource and status monitoring systems seems marginal, with the possible exception of monitoring soil moisture and major vegetation variations. The complementary nature of the scatterometer and radiometer systems was demonstrated by the correlation analysis. Although radiometers must have spatial resolutions dictated by antenna size, radars can use synthetic aperture techniques to achieve much finer resolutions. Multiplicity of modes in the S-193 sensors complicated both the system development and its employment. An attempt was made in the design of the S-193 to arrange optimum integration times for each angle and type of measurement. This unnecessarily complicated the design of the instrument, since the gains in precision achieved in this way were marginal. Either a software-controllable integration time or a set of only two or three integration times would have been better.

Moore, R. K.↗
NASA NTRS1977

Smithsonian Astrophysical Observatory laser tracking systems

The four SAO laser satellite-ranging systems, located in Brazil, Peru, Australia, and Arizona, have been in operation for more than five years and have provided ranging data at accuracy levels of a meter or better. The paper examines system hardware (laser transmitter, the electronics, mount, photoreceiver, minicomputer, and station timing) and software (prediction program, calibration programs, and data handling and quick-look programs) and also considers calibration, station operation, and system performance.

Pearlman, M. R.↗
NASA NTRS1977

Gamma-ray astronomy in the medium energy (10-50 MeV) range

To observe the medium energy component of the intense galactic center gamma-ray emission, two balloon flights of a medium energy gamma-ray spark chamber telescope were flown in Brazil in 1975. The results indicate the emission is higher than previously thought and above the predictions of a theoretical model proposed.

Kniffen, D. A.↗