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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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All sourcesNASA NTRSDOE OSTI
DOE OSTI2014

Beyond the Active Site: The Impact of the Outer Coordination Sphere on Electrocatalysts for Hydrogen Production and Oxidation

Hydrogenase enzymes provide inspiration for investigations of molecular catalysts utilizing structural and functional mimics of the active site. However, the resulting active site mimics cannot match the combination of high rates and low overpotentials of the enzyme, suggesting that the rest of the protein scaffold, i.e., the outer coordination sphere, is necessary for the efficiency of hydrogenase. Therefore, inclusion of outer coordination sphere elements onto molecular catalysts may enable us to achieve and ultimately surpass the overall enzymatic efficiency. In an effort to identify and include the missing enzymatic features, there has been recent effort to understand the effect of outer coordination sphere elements on molecular catalysts for hydrogen oxidation and production. Our focus has been to utilize amino acid or peptide based scaffolds on an active functional mimic for hydrogen oxidation and production, [Ni(PR2NR’2)2]2+. This bottom-up approach, i.e, building an outer coordination sphere around a functional molecular catalyst, has allowed us to evaluate individual contributions to catalysis, including enhancing proton movement, concentrating substrate and introducing structural features to control reactivity. Collectively, these studies have resulted in catalysts that can operate faster, can operate at lower overpotentials, have enhanced water solubility, and/or can provide more stability to oxygen or extreme conditions such as strongly acidic or basic conditions than their unmodified parent complexes. Common mechanisms have yet to be defined to predictably control these processes but our growing knowledge in this area is essential for the eventual mimicry of enzymes for developing efficient molecular catalysts for practical use. This account reviews previously published work supported by the US DOE Basic Energy Sciences (BES), Physical Bioscience program, the Office of Science Early Career Research Program through the USDOE, BES, the Center for Molecular Electrocatalysis, an Energy Frontier Research Center funded by the US DOE, Office of Science, Office of BES. Part of the research was conducted at the W.R. Wiley Environmental Molecular Sciences Laboratory, a national scientific user facility sponsored by U.S. DOE’s Office of Biological and Environmental Research program located at Pacific Northwest National Laboratory (PNNL). PNNL is operated by Battelle for the U.S. Department of Energy.

bioinspired↗
DOE OSTI2014

Oxidation of ethane to ethanol by N 2 O in a metal-organic framework with coordinatively unsaturated iron(II) sites

Enzymatic haem and non-haem high-valent iron–oxo species are known to activate strong C–H bonds, yet duplicating this reactivity in a synthetic system remains a formidable challenge. Although instability of the terminal iron–oxo moiety is perhaps the foremost obstacle, steric and electronic factors also limit the activity of previously reported mononuclear iron(IV)–oxo compounds. In particular, although nature's non-haem iron(IV)–oxo compounds possess high-spin S = 2 ground states, this electronic configuration has proved difficult to achieve in a molecular species. These challenges may be mitigated within metal–organic frameworks that feature site-isolated iron centres in a constrained, weak-field ligand environment. Here, we show that the metal–organic framework Fe 2 (dobdc) (dobdc 4- = 2,5-dioxido-1,4-benzenedicarboxylate) and its magnesium-diluted analogue, Fe 0.1 Mg 1.9 (dobdc), are able to activate the C–H bonds of ethane and convert it into ethanol and acetaldehyde using nitrous oxide as the terminal oxidant. Electronic structure calculations indicate that the active oxidant is likely to be a high-spin S = 2 iron(IV)–oxo species.

Xiao, Dianne J.↗
DOE OSTI2011

Asymmetric cation nonstoichiometry in spinels: Site occupancy in Co{sub 2}ZnO{sub 4} and Rh{sub 2}ZnO{sub 4}

Two cations A and B in A{sub 2}BO{sub 4} spinels appear in precise 2:1 Daltonian ratio ('line compounds') only at very low temperature. More typically, at finite temperature, they tend to become either A rich or B rich. Here we survey the experimentally observed stoichiometry asymmetries and describe the first-principles framework for calculating these. Defect calculations based on first principles are used to calculate the enthalpies of substitution of A atom {Delta}H(A{sub T{sub d}}) and B atom {Delta}H(B{sub O{sub h}}) and determine their site occupancies leading to (non)-stoichiometry. In Co{sub 2}ZnO{sub 4}, the result of the calculation for site occupancy compares well with that measured via anomalous x-ray diffraction. Further, the calculated phase boundary also compares well with that measured via Rietveld refinement of x-ray diffraction data on bulk ceramic sintered samples of Co{sub 2}ZnO{sub 4} and Rh{sub 2}ZnO{sub 4}. These results show that Co{sub 2}ZnO{sub 4} is heavily Co nonstoichiometric above 500 deg. C, whereas Rh{sub 2}ZnO{sub 4} is slightly Zn nonstoichiometric. We found that, in general, the calculated {Delta}H(A{sub T{sub d}}) is smaller than {Delta}H(B{sub O{sub h}}), if the A-rich competing phase is isostructural with the A{sub 2}BO{sub 4} host, for example, A{sub 2}AO{sub 4}, whereas B-rich competing phase is not, for example, BO. This observation is used to qualitatively explain nonstoichiometry and solid solutions observed in other spinels.

36 MATERIALS SCIENCE↗
DOE OSTI2020

Compilation of Site Names and Spatial Extents for Sites in the Hanford Site Disposition Database

Several analyses that will be completed as part of the Hanford Composite Analysis (CA) and Cumulative Impact Evaluation (CIE) require the extents of facilities, waste sites, and other locations on the Hanford Site. The purpose of this calculation is to provide spatial extents and consistent naming convention for those locations used as part of the analyses in a single dataset. This calculation documents the process used to develop the dataset.

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗
DOE OSTI2018

Initial Site-Specific Studies for Removing SNF from Six Shutdown Sites - 18042

The US Department of Energy (DOE) is exploring options for developing a large-scale transportation system for the safe transport of spent nuclear fuel (SNF) and high-level radioactive waste to future storage or disposal facilities. This complex, large-scale transportation system will involve coordinated, integrated activities. Interfaces with various transportation and non-transportation activities must be defined, and systems must be developed to ensure successful integration when the system becomes operational. To provide an understanding of the tasks and interfaces required for the complete de-inventory of particular sites where SNF is stored, DOE solicited bids from integrating contractors to develop initial site-specific de-inventory reports. The reports were developed for several nuclear power plant sites selected to represent a range of geographic locations, existing on-site SNF storage systems, and expected transportation modal options. Each report is presented in a self-contained narrative describing a potential campaign for removing all SNF and greater-than-class-C low-level radioactive waste (GTCC waste) from each specified site. Each study narrative includes site information; an analysis of the near-site transportation infrastructure; a concept of operations for the de-inventory; an estimated budget and schedule for the duration of operations; and a safety and security plan with site-specific considerations, emergency response considerations, and recommendations for the next steps DOE could take to de-inventory the site. The reports provide cost estimates for analytical purposes only; they do not attempt to specify the party or parties responsible for the costs. These reports present a first look at how an integrating contractor might recommend removing SNF from particular sites. They are written from the perspective of the contractor and do not represent DOE's plans. At this writing, six sites have been studied: - Connecticut Yankee, - Big Rock Point, - Humboldt Bay, - Maine Yankee, - Trojan, - Kewaunee. DOE may develop de-inventory reports for all reactor sites, with an initial focus on shutdown reactor sites. The order in which sites have been selected for study should not be considered an indicator of the order used by DOE to remove SNF from the sites. Since the SNF's destination is not important for the purpose of this work, DOE tasked the contractor with planning transportation of the SNF from the site to a generic railroad location in the approximate geographical center of the United States. These reports represent an initial study of the specific sites. Per the terms of the contract, the contractor was not permitted to engage in dialogue with power plant site personnel; near-site railroad personnel; or any local, state, or regional stakeholders about the studies. DOE provided site-specific data. The contractor was tasked to rely on past corporate experience, data available from DOE, and publicly available data when making site-specific recommendations for removing all SNF from each site. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗
DOE OSTI2016

Transition and Transfer of Remediated Formerly Utilized Sites Remedial Action Program (FUSRAP) Sites from U.S. Army Corps of Engineers (USACE) to U.S. Department of Energy (DOE)

The US Department of Energy (DOE) expects to receive the transfer of 10 FUSRAP Sites from the US Army Corps of Engineers (USACE) over the next 10 years; however, the timing of the transfers is highly dependent upon federal funding of the ongoing remedial actions. When remediation for each site is complete and the 2-year operations and maintenance period has concluded, each site will transfer from USACE to DOE for long-term surveillance and maintenance (LTS&M). US DOE’s Office of Legacy Management (LM) will accept program responsibility for these sites and conduct LTS&M activities required to maintain protectiveness, preserve site-specific knowledge, and retain the cleanup and stewardship records while keeping stakeholders informed. Since the last FUSRAP site transfer occurred in 2007, LM in coordination with USACE intends to establish a transition process to promote the seamless transfer of sites from the time when the first record of decision is signed to the completion of FUSRAP activities. The approach to transfer active FUSRAP sites to completed sites status has been historically outlined in foundational documents such as the 1999 Memorandum of Understanding and supporting letters of agreement between the two agencies. As more complex FUSRAP sites are completed, this transition process will provide a model between the two agencies to communicate future long-term care liabilities. Ultimately, the FUSRAP transition process is structured to acquire and preserve site knowledge and information necessary for protecting the environment and public health. As of 2015, LM has transitioned and accepted programmatic responsibility for over 90 sites. From LM’s perspective, successful transition of any site includes understanding the long-term environmental liabilities. LM uses site transition framework requirements from past transitions to develop site-specific transition plans. Site-specific transition plans are developed by LM in coordination with USACE and executed during the 2-year operations and maintenance period. An integrated project team of subject matter experts is assembled to address the conditions of the transitioning site; acquire a site records collection; evaluate site operations and final site conditions and associated risks; identify and contact stakeholders; and document the basis for site LTS&M requirements. While the majority of the transition activities are completed by LM, close coordination between US DOE LM and USACE throughout this process is essential for an effective and seamless transfer to assure that there is no lapse in site protectiveness.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2012

Nevada National Security Site Industrial Sites Project Closeout - 12498

The U.S. Department of Energy (DOE), National Nuclear Security Administration Nevada Site Office is responsible for environmental restoration (ER) at the Nevada National Security Site (NNSS). This includes remediation at Industrial Sites where past nuclear testing activities and activities that supported nuclear testing may have or are known to have resulted in the release of contaminants into the environment. Industrial Sites at the NNSS have included nuclear facilities that supported the nuclear rocket/missile development programs, gas stations, landfills, spill sites, ordnance sites, and numerous other waste disposal and release sites. The NNSS Industrial Sites activities neared completion at the end of fiscal year 2011 while other activities required under the Federal Facility Agreement and Consent Order (FFACO) and part of the same NNSS ER Project are forecasted to extend to 2027 or beyond. With the majority of Industrial Sites corrective action units (CAUs) completed (more than 250 CAUs and over 1,800 corrective action sites), it was determined that an activity closeout process should be implemented to ensure that the work completed over the past 15 years is well documented in a comprehensive and concise summary. While the process used to close each individual CAU is described in approved documents, no single document describes in summary fashion the work completed to close the many individual Industrial Sites. The activity closeout process will be used to develop an Industrial Sites closeout document that describes these years of work. This document will summarize the number of Industrial Sites closed under the FFACO and provide general descriptions of projects, contaminants removed, and sites closed in place with corresponding Use Restrictions. Other pertinent information related to Industrial Sites work such as the project history, closure decisions, historical declarations, remediation strategies, and final CAU status will be included in the closeout document, along with a table listing each CAU and corresponding corrective action sites within each CAU. Using this process of conducting the activity closeout and developing a closeout document may prove useful for other ER projects within the DOE complex in describing how a long period of ER can be summarized in a single document. The NNSS Industrial Sites activities were completed over the span of 15 years and involved the investigation, cleanup or Use Restriction, and closure of more than 260 CAUs and over 1,800 sites. These activities will conclude in FY 2012 (with the exception of one CAU). In order to capture the work completed over this length of time and document decisions made during the activities, a closeout effort was initiated. The closeout will review the work conducted during the Industrial Sites activities and produce a single document that summarizes Industrial Sites activities. This closeout is being conducted at an interim stage in the overall NNSA/NSO ER Project since the Soils and UGTA activities will continue for a number of years, but the completion of the Industrial Sites project warrants conducting a closeout now while personnel are available and information is still current. The process followed by NNSA/NSO in conducing project closeout for the Industrial Sites portion of the ER program may prove useful within the DOE complex in demonstrating how a large ER project can be summarized. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗
DOE OSTI2024

Recommendations for improving rigor and reproducibility in site specific characterization

Heterogeneous catalysis is driven by the interaction of reactant molecules and the catalyst surface. The locus of this interaction as well as the surrounding ensemble of atoms is referred to as the catalyst active site. Active site characterization attempts to distinguish active catalytic sites from inactive surface sites, to elucidate the structural and chemical nature of active sites, and to quantify active site concentration. Numerous techniques have been demonstrated to provide compositional and structural information about the active sites within a catalyst. However, each technique has its own limitations and experimental pitfalls that can lead to data misinterpretation or irreproducible results. Further, this work aims to provide an overview of the types of data that can be collected, to outline common experimental challenges and how to avoid them, and to assemble relevant references for the most used active site characterization techniques. More broadly, we aim to outline best practices for researchers to collect, interpret, and report active site characterization data in a way that provides the most benefit to the broader catalysis community. Increasing the rigor and reproducibility of active site characterization offers a strategy to better link properties with catalytic performance and to enable the community to develop consensus concerning these relationships.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
DOE OSTI2013

Monitoring Soil Erosion on a Burned Site in the Mojave-Great Basin Transition Zone: Final Report for the Jacob Fire Site

A historic return interval of 100 years for large fires in the U.S. southwestern deserts is being replaced by one where fires may reoccur as frequently as every 20 to 30 years. The shortened return interval, which translates to an increase in fires, has implications for management of Soil Corrective Action Units (CAUs) and Corrective Action Sites (CASs) for which the Department of Energy, National Nuclear Security Administration Nevada Field Office has responsibility. A series of studies was initiated at uncontaminated analog sites to better understand the possible impacts of erosion and transport by wind and water should contaminated soil sites burn. The first of these studies was undertaken at the Jacob Fire site approximately 12 kilometers (7.5 miles) north of Hiko, Nevada. A lightning-caused fire burned approximately 200 hectares during August 6-8, 2008. The site is representative of a transition between Mojave and Great Basin desert ecoregions on the Nevada National Security Site (NNSS), where the largest number of Soil CAUs/CASs are located. The area that burned at the Jacob Fire site was primarily a Coleogyne ramosissima (blackbrush) and Ephedra nevadensis (Mormon tea) community, also an abundant shrub assemblage in the similar transition zone on the NNSS. This report summarizes three years of measurements after the fire. Seven measurement campaigns at the Jacob Fire site were completed. Measurements were made on burned ridge (upland) and drainage sites, and on burned and unburned sites beneath and between vegetation. A Portable In-Situ Wind Erosion Lab (PI-SWERL) was used to estimate emissions of suspended particles at different wind speeds. Context for these measurements was provided through a meteorological tower that was installed at the Jacob Fire site to obtain local, relevant environmental parameters. Filter samples, collected from the exhaust of the PI-SWERL during measurements, were analyzed for chemical composition. Runoff and water erosion were quantified through a series of rainfall/runoff simulation tests in which controlled amounts of water were delivered to the soil surface in a specified amount of time. Runoff data were collected from understory and interspace soils on burned ridge and drainage areas. Runoff volume and suspended sediment in the runoff were sampled; the particle size distribution of the sediment was determined by laboratory analysis. Several land surface and soil characteristics associated with runoff were integrated by the calculation of site-specific curve numbers. Several vegetation surveys were conducted to assess post-burn recovery. Data from plots in both burned and unburned areas included species identification, counts, and location. Characterization of fire-affected area included measures at both the landscape scale and at specific sites. Although wind erosion measurements indicate that there are seasonal influences on almost all parameters measured, several trends were observed. PI-SWERL measurements indicated the potential for PM 10 windblown dust emissions was higher on areas that were burned compared to areas that were not. Among the burned areas, understory soils in drainage areas were the most emissive, and interspace soils along burned ridges were least emissive. By 34 months after the burn (MAB), at the end of the study, emissions from all burned soil sites were virtually indistinguishable from unburned levels. Like the amount of emissions, the chemical signature of the fire (indicated by the EC-Soil ratio) was elevated immediately after the fire and approached pre-burn levels by 24 MAB. Thus, the potential for wind erosion at the Jacob Fire site, as measured by the amount and type of emissions, increased significantly after the fire and returned to unburned levels by 24 MAB. The effect of fire on the potential for water erosion at the Jacob Fire site was more ambiguous. Runoff and sediment from ridge interspace soils and unburned interspace soils were similar throughout the study period. Seldom, if ever, did runoff and sediment occur in burned drainage area soils. For burned soils where runoff occurred at 1 MAB, the sediment size was finer than on unburned sites, but this effect disappeared by 3 MAB. For the three year study under the conditions tested at the Jacob Fire site, the potential for water erosion appeared relatively unaffected by the fire. Vegetation responses were documented for each year following the fire. By the end of the study, there was a substantial difference in plant densities and richness between drainage and ridge sites. Cheatgrass densities were higher in unburned plots, and cheatgrass was also more dominant in the community composition in unburned plots. Cheatgrass had increased in the burned area but so did other native species. Three years after the fire, the burned landscape continued to revegetate but had yet to approximate the condition of an unburned landscape. The results from the vegetation surveys support the wind erosion results, where the primary source of windborne particles originate from the understory, where lower plant diversity and densities were found. The soil appears to be more resilient and have a much shorter recovery time than the vegetation in this particular community.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2013

Closure Report for Corrective Action Unit 104: Area 7 Yucca Flat Atmospheric Test Sites, Nevada National Security Site, Nevada

This Closure Report (CR) presents information supporting closure of Corrective Action Unit (CAU) 104, Area 7 Yucca Flat Atmospheric Test Sites, and provides documentation supporting the completed corrective actions and confirmation that closure objectives for CAU 104 were met. This CR complies with the requirements of the Federal Facility Agreement and Consent Order (FFACO) that was agreed to by the State of Nevada; the U.S. Department of Energy (DOE), Environmental Management; the U.S. Department of Defense; and DOE, Legacy Management. CAU 104 consists of the following 15 Corrective Action Sites (CASs), located in Area 7 of the Nevada National Security Site: · CAS 07-23-03, Atmospheric Test Site T-7C · CAS 07-23-04, Atmospheric Test Site T7-1 · CAS 07-23-05, Atmospheric Test Site · CAS 07-23-06, Atmospheric Test Site T7-5a · CAS 07-23-07, Atmospheric Test Site - Dog (T-S) · CAS 07-23-08, Atmospheric Test Site - Baker (T-S) · CAS 07-23-09, Atmospheric Test Site - Charlie (T-S) · CAS 07-23-10, Atmospheric Test Site - Dixie · CAS 07-23-11, Atmospheric Test Site - Dixie · CAS 07-23-12, Atmospheric Test Site - Charlie (Bus) · CAS 07-23-13, Atmospheric Test Site - Baker (Buster) · CAS 07-23-14, Atmospheric Test Site - Ruth · CAS 07-23-15, Atmospheric Test Site T7-4 · CAS 07-23-16, Atmospheric Test Site B7-b · CAS 07-23-17, Atmospheric Test Site - Climax Closure activities began in October 2012 and were completed in April 2013. Activities were conducted according to the Corrective Action Decision Document/Corrective Action Plan for CAU 104. The corrective actions included No Further Action and Clean Closure. Closure activities generated sanitary waste, mixed waste, and recyclable material. Some wastes exceeded land disposal limits and required treatment prior to disposal. Other wastes met land disposal restrictions and were disposed in appropriate onsite landfills. The U.S. Department of Energy, National Nuclear Security Administration Nevada Field Office (NNSA/NFO) requests the following: · A Notice of Completion from the Nevada Division of Environmental Protection to NNSA/NFO for closure of CAU 104 · The transfer of CAU 104 from Appendix III to Appendix IV, Closed Corrective Action Units, of the FFACO

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗
DOE OSTI2018

Evaluation of Potential Dose to Off-site Receptors from On-site Removable Contamination - 18521

Calculations used in making corrective action decisions estimate the maximum potential dose to on-site receptors exposed to contamination from nuclear testing activities at U.S. Department of Energy, Environmental Management Nevada Program Soils Activity release sites. At many of these release sites, removable contamination is present at levels that require controls to be in place under Title 10 Code of Federal Regulations Part 835, 'Occupational Radiation Protection.' When significant levels of removable contamination are present at a release site, it may be necessary to evaluate the potential dose to off-site receptors from removable contamination that has the potential to be transported off the contaminated site. The protocols described herein could be used to evaluate the potential dose to off-site receptors to ensure that corrective action decisions consider both on-site and off-site potential receptors. The calculation of estimated dose to a receptor can be accomplished by establishing a scenario that describes the exposure (e.g., time and pathway) of the individual to the radiological contamination and by using the properties of the contaminants (e.g., dose conversion factors). Radiological contamination transported from the site has the potential to expose off-site receptors under a potentially large number of exposure scenarios that cannot be comprehensively defined. However, a scenario was developed to represent a maximally exposed off-site receptor. Because the estimated dose to the maximally exposed off-site receptor based on maximum post-closure release site activity levels is less than the on-site dose decision level, corrective action decisions at the Nevada National Security Site Soils Activity release sites are also protective of potential off-site receptors when significant levels of removable contamination are present. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗
DOE OSTI2016

On the applicability of surrogate-based Markov chain Monte Carlo-Bayesian inversion to the Community Land Model: Case studies at flux tower sites: SURROGATE-BASED MCMC FOR CLM

The Community Land Model (CLM) has been widely used in climate and Earth system modeling. Accurate estimation of model parameters is needed for reliable model simulations and predictions under current and future conditions, respectively. In our previous work, a subset of hydrological parameters has been identified to have significant impact on surface energy fluxes at selected flux tower sites based on parameter screening and sensitivity analysis, which indicate that the parameters could potentially be estimated from surface flux observations at the towers. To date, such estimates do not exist. In this paper, we assess the feasibility of applying a Bayesian model calibration technique to estimate CLM parameters at selected flux tower sites under various site conditions. The parameters are estimated as a joint probability density function (PDF) that provides estimates of uncertainty of the parameters being inverted, conditional on climatologically-average latent heat fluxes derived from observations. We find that the simulated mean latent heat fluxes from CLM using the calibrated parameters are generally improved at all sites when compared to those obtained with CLM simulations using default parameter sets. Further, our calibration method also results in credibility bounds around the simulated mean fluxes which bracket the measured data. The modes (or maximum a posteriori values) and 95% credibility intervals of the site-specific posterior PDFs are tabulated as suggested parameter values for each site. Analysis of relationships between the posterior PDFs and site conditions suggests that the parameter values are likely correlated with the plant functional type, which needs to be confirmed in future studies by extending the approach to more sites.

Huang, Maoyi↗
DOE OSTI2018

A transient post-translational modification of active site cysteine alters binding properties of the parkinsonism protein DJ-1

Highlights: • A fraction of bacterially expressed polyhistidine-tagged human DJ-1 tightly binds to Ni-nitrilotriacetate (Ni-NTA) column. • The color of Ni-NTA column changes from light cyan to blue violet upon tight binding to DJ-1. • An active site cysteine is post-translationally modified in the fraction of DJ-1 tightly bound to Ni-NTA. • The modificationconferring tightbinding to Ni-NTA is unstable. • Crystal structure shows the unstable modification converts to S-carboxymethylcysteine upon elution from Ni-NTA. Mutations in the human protein DJ-1 cause early onset of Parkinson's disease. A reactive cysteine residue (Cys{sup 106}) of DJ-1 is crucial for its protective function, although the underlying mechanisms are unclear. Here we show that a fraction of bacterially expressed polyhistidine-tagged human DJ-1 could not be eluted from a Ni-nitrilotriacetate (Ni-NTA) column with 150 mM imidazole. This unusually tight binding was accompanied by the appearance of blue violet color on the Ni-NTA column. We demonstrate by X-ray crystallography that Cys{sup 106} is carboxymethylated in a fraction of DJ-1 tightly bound to Ni-NTA and that the replacement of Cys{sup 106} by serine abrogates the tight binding and the appearance of blue violet color. However, carboxymethylation of purified DJ-1 is insufficient to confer the tight binding to Ni-NTA. Moreover, when eluted protein was re-applied to the Ni-NTA column, no tight binding was observed, indicating that the formation of high affinity complex with Ni-NTA depends on a transient modification of Cys{sup 106} that transforms into a Cys{sup 106}-carboxymethyl adduct upon elution from Ni-NTA. We conclude that an unknown metabolite reacts with Cys{sup 106} of DJ-1 to result in a transient post-translational modification. This modification is distinct from simple oxidation to sulfinic or sulfenic acids and confers altered binding properties to DJ-1 suggesting that it could serve as a signal for sensing oxidant stress.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2023

Lawrence Livermore National Laboratory Experimental Test Site 300 (Site 300): Site 300 Roadway Improvements - Chem Mag Loop Soil Sampling and Analysis Plan (May 2023)

This Soil Sampling and Analysis Plan (SAP) was prepared by the Environmental Function Area (EFA)/Technical Services Department (TSD) of the Environment, Safety & Health (ES&H) Directorate for the Project Management Office (PMO) for the proposed Roadway Improvements Project at the Chem Mag Loop (project). The purpose of the SAP is to describe the procedures for collection and analysis of environmental samples and evaluation of analytical data (chemical and radiological) to determine management options of excavated soil during project construction. This SAP follows criteria established in Lawrence Livermore National Laboratory’s (LLNL) Soils Screening and Management Plan (SSMP) (LLNL 2022), which was formalized in accordance with U.S. Environmental Protection Agency (EPA) guidance for developing Data Quality Objectives for environmental data (EPA 2006) and the Multi-Agency Radiation Survey and Site Investigation Manual (MARSSIM) guidance (U.S. NRC, U.S. EPA, U.S. DOE, and U.S. DOD 2000). The scope of this SAP is based on preliminary design information provided to EFA by PMO.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2023

Lawrence Livermore National Laboratory Experimental Test Site 300 (Site 300): Site 300 Roadway Improvements - 836 Complex Soil Sampling and Analysis Plan (May 2023)

This Soil Sampling and Analysis Plan (SAP) was prepared by the Environmental Functional Area (EFA)/Technical Services Department (TSD) of the Environment, Safety & Health (ES&H) Directorate for the Project Management Office (PMO) for the proposed Roadway Improvements Project at the 836 Complex (project). The purpose of the SAP is to describe the procedures for collection and analysis of environmental samples and evaluation of analytical data (chemical and radiological) to determine management options of excavated soil during project construction. This SAP follows criteria established in Lawrence Livermore National Laboratory’s (LLNL) Soils Screening and Management Plan (SSMP) (LLNL 2022), which was formalized in accordance with U.S. Environmental Protection Agency (EPA) guidance for developing Data Quality Objectives for environmental data (EPA 2006) and the Multi-Agency Radiation Survey and Site Investigation Manual (MARSSIM) guidance (U.S. NRC, U.S. EPA, U.S. DOE, and U.S. DOD 2000). The scope of this SAP is based on preliminary design information provided by PMO.

54 ENVIRONMENTAL SCIENCES↗
NASA NTRS1975

Soil moisture ground truth, Lafayette, Indiana, site; St. Charles Missouri, site; Centralia, Missouri, site

The soil moisture ground-truth measurements and ground-cover descriptions taken at three soil moisture survey sites located near Lafayette, Indiana; St. Charles, Missouri; and Centralia, Missouri are given. The data were taken on November 10, 1975, in connection with airborne remote sensing missions being flown by the Environmental Research Institute of Michigan under the auspices of the National Aeronautics and Space Administration. Emphasis was placed on the soil moisture in bare fields. Soil moisture was sampled in the top 0 to 1 in. and 0 to 6 in. by means of a soil sampling push tube. These samples were then placed in plastic bags and awaited gravimetric analysis.

Jones, E. B.↗
DOE OSTI2014

2013 Annual Site Inspection and Monitoring Report for Uranium Mill Tailings Radiation Control Act Title I Disposal Sites

This report, in fulfillment of a license requirement, presents the results of long-term surveillance and maintenance activities conducted by the U.S. Department of Energy (DOE) Office of Legacy Management (LM) in 2013 at 19 uranium mill tailings disposal sites established under Title I of the Uranium Mill Tailings Radiation Control Act (UMTRCA) of 1978.1 These activities verified that the UMTRCA Title I disposal sites remain in compliance with license requirements. DOE operates 18 UMTRCA Title I sites under a general license granted by the U.S. Nuclear Regulatory Commission (NRC) in accordance with Title 10 Code of Federal Regulations Part 40.27 (10 CFR 40.27). As required under the general license, a long-term surveillance plan (LTSP) for each site was prepared by DOE and accepted by NRC. The Grand Junction, Colorado, Disposal Site, one of the 19 Title I sites, will not be included under the general license until the open, operating portion of the cell is closed. The open portion will be closed either when it is filled or in 2023. This site is inspected in accordance with an interim LTSP. Long-term surveillance and maintenance services for these disposal sites include inspecting and maintaining the sites; monitoring environmental media and institutional controls; conducting any necessary corrective actions; and performing administrative, records, stakeholder relations, and other regulatory stewardship functions. Annual site inspections and monitoring are conducted in accordance with site-specific LTSPs and procedures established by DOE to comply with license requirements. Each site inspection is performed to verify the integrity of visible features at the site; to identify changes or new conditions that may affect the long-term performance of the site; and to determine the need, if any, for maintenance, follow-up or contingency inspections, or corrective action in accordance with the LTSP. LTSPs and site compliance reports are available on the Internet at http://www.lm.doe.gov/.

2013↗