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At least 55 records · Page 3

Mars Observer mission plan

The paper describes plans devised for operating the Mars Observer mission during its five phases of activity: the launch, the cruise, the orbit insertion, the mapping, and the Mars 1994 support. The latter phase is to provide a data relay for a Sovier mission to Mars which will be launched in 1994. These plans will be used to plan detailed sequencing strategies for the Mars Observer mission. The low-altitude orbit and a full Martian year of observations will provide, both in temporal and spatial coverage and resolution, the most comprehensive study of an extraterrestrial planet to date.

Blume, William H.↗

Architecture for robot intelligence

An architecture for robot intelligence enables a robot to learn new behaviors and create new behavior sequences autonomously and interact with a dynamically changing environment. Sensory information is mapped onto a Sensory Ego-Sphere (SES) that rapidly identifies important changes in the environment and functions much like short term memory. Behaviors are stored in a DBAM that creates an active map from the robot's current state to a goal state and functions much like long term memory. A dream state converts recent activities stored in the SES and creates or modifies behaviors in the DBAM.

Peters, II, Richard Alan↗

Apparatus for multiprocessor-based control of a multiagent robot

An architecture for robot intelligence enables a robot to learn new behaviors and create new behavior sequences autonomously and interact with a dynamically changing environment. Sensory information is mapped onto a Sensory Ego-Sphere (SES) that rapidly identifies important changes in the environment and functions much like short term memory. Behaviors are stored in a DBAM that creates an active map from the robot's current state to a goal state and functions much like long term memory. A dream state converts recent activities stored in the SES and creates or modifies behaviors in the DBAM.

Peters, II, Richard Alan↗

Multiomics and deep learning dissect regulatory syntax in human development

Transcription factors establish cell identity during development by binding regulatory DNA in a sequence-specific manner, often promoting local chromatin accessibility and regulating gene expression1. Mapping accessible chromatin offers critical insights into transcriptional control, but available datasets for human development are restricted to bulk tissue, single organs or single modalities2. Here we present the Human Development Multiomic Atlas, a single-cell atlas of chromatin accessibility and gene expression from 817,740 fetal cells across 12 organs, spanning 203 cell types and more than 1 million candidate cis-regulatory elements, many of which exhibit organ-specific in vivo enhancer activity. Deep learning models trained to predict accessibility from local DNA sequence unravel a comprehensive lexicon of motifs that influence accessibility, including composite motifs exhibiting distinct syntactic constraints that are predicted to mediate transcription factor cooperativity. We identify ‘hard’ syntactic rules requiring precise motif spacing and orientation, ‘soft’ rules allowing flexible motif arrangements, and ubiquitous motifs inhibiting accessibility. Model-based interpretation of genetic variants reveals that disruption of motifs with positive and negative effects is associated with concordant effects on gene expression. Our work delineates how motif syntax governs cell-type-specific chromatin accessibility and provides a foundational resource for decoding cis-regulatory logic and interpreting genetic variation during human development.

59 BASIC BIOLOGICAL SCIENCES↗

Hierarchical Chiral Self-Assembly of Nanocylinders Composed of Sequence-Defined Mesogenic Dimers

Chiral ensembles can arise through supramolecular curvature that resolves geometric frustrations in the packing of bent, achiral molecular or colloidal building blocks. Here, we leverage orthogonal protection−deprotection click chemistry to create sequence-defined mesogenic heterodimers exhibiting emergent chirality. We compare the hierarchical self-assembly of the synthesized asymmetric, achiral heterodimers, which differ only in the position of a methyl substituent. Both dimers form chiral spherulites composed of nanocylinders. However, the detailed arrangement of nanocylinders depends on the position of the methyl substituent and the crystallization conditions. Despite the chemical similarity, in one dimer, two crystalline forms are optically active. They form conglomerates of dextrorotatory and levorotatory spherulites. The other dimer forms more highly anisotropic spherulites that mask circular birefringence arising from the misorientation of nanocylinders, while mapping of nanocylinder directors reveals a sense at the spherulite surface. We propose that differences in nanocylinder arrangements may arise from changes in nanocylinder curvature and dimensions dictated by the methyl substituent position, inducing chirality. These results demonstrate multiscale hierarchical assembly relevant to dense systems of tubular structures and highlight the role of sequence and molecular design in directing the bottom-up hierarchical self-assembly and chirality of mesogenic systems.

Alkyls↗

Infrared and millimeter-wave observations of the Sharpless 156 molecular cloud

Comprehensive near-infrared, far-infrared, and CO measurements of the Sharpless 156 region reveal five distinct sources embedded in a giant molecular cloud. These embedded sources, which appear to have formed independently within the cloud, form a diverse collection of objects; these include a prestellar condensation, massive stars with associated reflection and emission nebulosity, a bright compact H II region, and possibly even a luminous, post-main-sequence M star. The majority of the cloud has been mapped in (C-12)O and (C-13)O and has been surveyed at 2.2 microns. Also presented are 40-160 micron far-infrared maps and 1-20 micron near-infrared photometry of the active regions along with 6 arcsec resolution 10 micron and 20 micron maps of the central nebula. Inquiry is made into the nature of each source and into the process of star formation for the complex as a whole.

Joy, M.↗

Architecture for Multiple Interacting Robot Intelligences

An architecture for robot intelligence enables a robot to learn new behaviors and create new behavior sequences autonomously and interact with a dynamically changing environment. Sensory information is mapped onto a Sensory Ego-Sphere (SES) that rapidly identifies important changes in the environment and functions much like short term memory. Behaviors are stored in a database associative memory (DBAM) that creates an active map from the robot's current state to a goal state and functions much like long term memory. A dream state converts recent activities stored in the SES and creates or modifies behaviors in the DBAM.

Peters, Richard Alan, II↗

High Throughput expression and characterization of laccases in Saccharomyces cerevisiae

Laccases are oxidative enzymes containing 4 conserved copper heteroatoms. Laccases catalyze cleavage of bonds in lignin using radical chemistry, yet their exact specificity for bonds (such as the β-O-4 or C-C) in lignin remains unknown and may vary with the diversity of laccases across fungi, plants and bacteria. Bond specificity may perhaps even vary for the same enzyme across different reaction conditions. Determining these differences has been difficult due to the fact that heterologous expression of soluble, active laccases has proven difficult. Here we describe the successful heterologous expression of functional laccases in two strains of Saccharomyces cerevisiae, including one we genetically modified with CRISPR. We phylogenically map the enzymes that we successfully expressed, compared to those that did not express. We also describe differences protein sequence differences and pH and temperature profiles and their ability to functionally express, leading to a potential future screening platform for directed evolution of laccases and other ligninolytic enzymes such as peroxidases.

59 BASIC BIOLOGICAL SCIENCES↗

Genome-scale model development and genomic sequencing of the oleaginous clade Lipomyces

The Lipomyces clade contains oleaginous yeast species with advantageous metabolic features for biochemical and biofuel production. Limited knowledge about the metabolic networks of the species and limited tools for genetic engineering have led to a relatively small amount of research on the microbes. Here, a genome-scale metabolic model (GSM) of Lipomyces starkeyi NRRL Y-11557 was built using orthologous protein mappings to model yeast species. Phenotypic growth assays were used to validate the GSM (66% accuracy) and indicated that NRRL Y-11557 utilized diverse carbohydrates but had more limited catabolism of organic acids. The final GSM contained 2,193 reactions, 1,909 metabolites, and 996 genes and was thus named iLst996. The model contained 96 of the annotated carbohydrate-active enzymes. iLst996 predicted a flux distribution in line with oleaginous yeast measurements and was utilized to predict theoretical lipid yields. Twenty-five other yeasts in the Lipomyces clade were then genome sequenced and annotated. Sixteen of the Lipomyces species had orthologs for more than 97% of the iLst996 genes, demonstrating the usefulness of iLst996 as a broad GSM for Lipomyces metabolism. Pathways that diverged from iLst996 mainly revolved around alternate carbon metabolism, with ortholog groups excluding NRRL Y-11557 annotated to be involved in transport, glycerolipid, and starch metabolism, among others. Overall, this study provides a useful modeling tool and data for analyzing and understanding Lipomyces species metabolism and will assist further engineering efforts in Lipomyces .

59 BASIC BIOLOGICAL SCIENCES↗

ObstacleSense: Low-Power Neuromorphic Vision for Corridor Obstacle Awareness in Low-Level ADAS

The automotive industry’s pursuit of Level 5 autonomy is constrained by substantial perception-compute power requirements, often reaching 1, 000 + watts in full autonomy stacks. Reducing this energy burden requires rethinking perception not only at the high-end autonomy level, but also at the foundational Advanced Driver Assistance Systems (ADAS) level where low-power, safety-critical sensing can have broad impact. Neuromorphic vision provides a promising starting point: HD Dynamic Vision Sensors (DVS) can operate below 100 mW at the sensor level by reporting only asynchronous brightness changes. However, low-power sensing alone is insufficient if downstream perception reintroduces dense, energy-intensive computation. In particular, many event-driven object-detection pipelines still rely on CNN backbones, while purely spiking alternatives often trade away accuracy or ignore deployment constraints. We introduce ObstacleSense, a highly compact, CNN-free hybrid ANN–SNN framework for Level 0–1 forward-corridor obstacle awareness. Instead of performing full-scene object detection with a convolutional feature backbone, ObstacleSense targets the safety-critical question of whether the ego corridor is occupied and how far the nearest obstacle is. The architecture combines polarity-conditioned event encoding, lightweight temporal spiking dynamics, axial spatial mixing, and coarse-to-fine range estimation within a regular fixed-grid compute pattern. This design avoids the dense CNN backbone commonly used in event-based detection while maintaining a small state footprint suitable for eventual small-FPGA deployment. Before hardware mapping, we evaluate the software implementation using a model-side power proxy derived from MACs, weight and activation traffic, and spiking state updates under shared FP16 assumptions. On simulated CARLA event corpora, the deployment-oriented model achieves 0.9464 objectness F1, 0.9978 grid-level mAP, and 0.8987 m distance Mean Absolute Error at an estimated 1.92 mW proxy cost, while maintaining performance on unseen generalization test sequences.

Johnson-Scott, Zac [ORNL]↗

A Germline-Targeting Chimpanzee SIV Envelope Glycoprotein Elicits a New Class of V2-Apex Directed Cross-Neutralizing Antibodies

HIV-1 and its SIV precursors share a broadly neutralizing antibody (bNAb) epitope in variable loop 2 (V2) at the envelope glycoprotein (Env) trimer apex. Here, we tested the immunogenicity of germ line-targeting versions of a chimpanzee SIV (SIVcpz) Env in human V2-apex bNAb heavy-chain precursor-expressing knock-in mice and as chimeric simian-chimpanzee immunodeficiency viruses (SCIVs) in rhesus macaques (RMs). Trimer immunization of knock-in mice induced V2-directed NAbs, indicating activation of V2-apex bNAb precursor-expressing mouse B cells. SCIV infection of RMs elicited high-titer viremia, potent autologous tier 2 neutralizing antibodies, and rapid sequence escape in the canonical V2-apex epitope. Six of seven animals also developed low-titer heterologous plasma breadth that mapped to the V2-apex. Antibody cloning from two of these animals identified multiple expanded lineages with long heavy chain third complementarity determining regions that cross-neutralized as many as 7 of 19 primary HIV-1 strains, but with low potency. Negative stain electron microscopy (NSEM) of members of the two most cross-reactive lineages confirmed V2 targeting but identified an angle of approach distinct from prototypical V2-apex bNAbs, with antibody binding either requiring or inducing an occluded-open trimer. Probing with conformation-sensitive, nonneutralizing antibodies revealed that SCIV-expressed, but not wild-type SIVcpz Envs, as well as a subset of primary HIV-1 Envs, preferentially adopted a more open trimeric state. These results reveal the existence of a cryptic V2 epitope that is exposed in occluded-open SIVcpz and HIV-1 Env trimers and elicits cross-neutralizing responses of limited breadth and potency.

59 BASIC BIOLOGICAL SCIENCES↗

Assessment of computer techniques for processing digital LANDSAT MSS data for lithological discrimination of Serra do Ramalho, State of Bahia

Enhancement techniques and thematic classifications were applied to the metasediments of Bambui Super Group (Upper Proterozoic) in the Region of Serra do Ramalho, SW of the state of Bahia. Linear contrast stretch, band-ratios with contrast stretch, and color-composites allow lithological discriminations. The effects of human activities and of vegetation cover mask and limit, in several ways, the lithological discrimination with digital MSS data. Principal component images and color composite of linear contrast stretch of these products, show lithological discrimination through tonal gradations. This set of products allows the delineations of several metasedimentary sequences to a level superior to reconnaissance mapping. Supervised (maximum likelihood classifier) and nonsupervised (K-Means classifier) classification of the limestone sequence, host to fluorite mineralization show satisfactory results.

Paradella, W. R.↗

Converting from CVF to AAF

A computer program called dsn config converter automates what had been a manual process for updating the multimission adaptation file (multi.aaf) used by a multiple-mission-command-sequence-generating process comprised of a combination of the AUTOGEN and APGEN programs mentioned in the immediately preceding article. The program converts the dsn_config.cvf file that provides DSN (Deep Space Network) antenna configuration code mappings from a context variable file (CVF) format used in another part of the command generation process to an APGEN activity file (AAF) format used by AUTOGEN and APGEN. Whereas previously, the information in the dsn_config.cvf file was manually encoded into the multi.aaf file, now the program automatically generates a dsn_config.aaf file from the dsn_config.cvf file. As part of this development effort the multi.aaf file was adapted to use the new dsn_config.aaf representations. Through this automation a tedious error-prone step has now been replaced by a quick and robust step.

Gladden, Roy E.↗

Evidence for a complex archean deformational history; southwestern Michipicoten Greenstone Belt, Ontario

The Michipicoten Greenstone Belt extends for about 150 km ENE from the northeastern angle of Lake Superior. In common with many other Archean greenstone belts, it is characterized by generally steep bedding dips and a distribution of major lithologic types suggesting a crudely synclinal structure for the belt as a whole. Detailed mapping and determination of structural sequence demonstrates that the structure is much more complex. The Archean history of the belt includes formation of at least three regionally significant cleavages, kilometer-scale overturning, extensive shearing, and diabase intrusion. Most well defined, mappable 'packages' of sedimentary rocks appear to be bounded by faults. These faults were active relatively early in the structural history of the belt, when extensive overturning also occurred. Steepening of dips, NW-SE shortening, development of steep NE cleavage, and pervasive shearing all postdate the early faulting and the regional overturning, obscuring much of the detail needed to define the geometry of the earlier structures. The results obtained so far suggest, however, that the Michipicoten Greenstone Belt underwent an early stage of thrusting and associated isoclinal folding, probably in a convergent tectonic environment.

Mcgill, George E.↗

ALMA Observations of α Centauri First Detection of Main-Sequence Stars at 3 mm Wavelength

The precise mechanisms that provide the non-radiative energy for heating the chromosphere and the corona of the Sun and those of other stars constitute an active field of research. By studying stellar chromospheres one aims at identifying the relevant physical processes. Defining the permittable extent of the parameter space can also serve as a template for the Sun-as-a-star. This feedback will probably also help identify stars that potentially host planetary systems that are reminiscent of our own. Earlier observations with Herschel and APEX have revealed the temperature minimum of Alpha Cen, but these were unable to spatially resolve the binary into individual components. With the data reported in this Letter, we aim at remedying this shortcoming. Furthermore, these earlier data were limited to the wavelength region between 100 and 870 um. In the present context, we intend to extend the spectral mapping (SED) to longer wavelengths, where the contrast between stellar photospheric and chromospheric emission becomes increasingly evident. The Atacama Large Millimeter submillimeter Array (ALMA) is particularly suited to point sources, such as unresolved stars. ALMA provides the means to achieve our objectives with both its high sensitivity of the collecting area for the detection of weak signals and the high spatial resolving power of its adaptable interferometer for imaging close multiple stars. This is the first detection of main-sequence stars at a wavelength of 3 mm. Furthermore, the individual components of the binary Alpha CenAB are clearly detected and spatially well resolved at all ALMA wavelengths. The high signal-to-noise ratios of these data permit accurate determination of their relative flux ratios, i.e., S(sub y(sup B)) / S(sub y(sup A))> = 0.54 +/- 0.04 at 440 μm, = 0.46 +/- 0.01 at 870 μm, and = 0.47 +/- 0.006 at 3.1 mm, respectively.. The previously obtained flux ratio of 0.44+/-0.18, which was based on measurements in the optical and at 70 μm, is consistent with the present ALMA results, albeit with a large error bar. The observed 3.1 mm emission greatly exceeds what is predicted from the stellar photospheres, and undoubtedly arises predominantly as free-free emission in the ionized chromospheric plasmas of both stars. Given the distinct difference in their cyclic activity, the similarity of their submm SEDs appears surprising.

Herschel↗

Genome-Wide Analysis of Terpene Synthase Gene Family in Mentha longifolia and Catalytic Activity Analysis of a Single Terpene Synthase

Terpenoids are a wide variety of natural products and terpene synthase (TPS) plays a key role in the biosynthesis of terpenoids. Mentha plants are rich in essential oils, whose main components are terpenoids, and their biosynthetic pathways have been basically elucidated. However, there is a lack of systematic identification and study of TPS in Mentha plants. In this work, we genome-widely identified and analyzed the TPS gene family in Mentha longifolia, a model plant for functional genomic research in the genus Mentha. A total of 63 TPS genes were identified in the M. longifolia genome sequence assembly, which could be divided into six subfamilies. The TPS-b subfamily had the largest number of genes, which might be related to the abundant monoterpenoids in Mentha plants. The TPS-e subfamily had 18 members and showed a significant species-specific expansion compared with other sequenced Lamiaceae plant species. The 63 TPS genes could be mapped to nine scaffolds of the M. longifolia genome sequence assembly and the distribution of these genes is uneven. Tandem duplicates and fragment duplicates contributed greatly to the increase in the number of TPS genes in M. longifolia. The conserved motifs (RR(X)8W, NSE/DTE, RXR, and DDXXD) were analyzed in M. longifolia TPSs, and significant differentiation was found between different subfamilies. Adaptive evolution analysis showed that M. longifolia TPSs were subjected to purifying selection after the species-specific expansion, and some amino acid residues under positive selection were identified. Furthermore, we also cloned and analyzed the catalytic activity of a single terpene synthase, MlongTPS29, which belongs to the TPS-b subfamily. MlongTPS29 could encode a limonene synthase and catalyze the biosynthesis of limonene, an important precursor of essential oils from the genus Mentha. This study provides useful information for the biosynthesis of terpenoids in the genus Mentha.

59 BASIC BIOLOGICAL SCIENCES↗

Mars 2020 – Landing a 1-ton rover and helicopter in an ancient Martian Lake

The Mars 2020 spacecraft launched in July 2020 and landed the Perseverance rover and Ingenuity helicopter successfully in Jezero crater on Feb. 18, 2021. Mars 2020 is the first stage of the Mars Sample Return campaign that will bring back the first samples from another planet to Earth. The entry, descent, and landing (EDL) sequence of the Mars 2020 spacecraft largely leveraged the previous Mars Science Laboratory (MSL) mission from 2012. Mars 2020 retained most of the EDL sequences of MSL, including active maneuvering during hypersonic flight to accurately target the landing site and use of the Skycrane descent stage that slowly lowered the rover while hovering above the ground. But Mars 2020 also added Terrain Relative Navigation, a machine vision-based system that allowed the spacecraft to navigate using an on-board camera that mapped ground landmarks to an on-board map, allowing the spacecraft to safely land in locations that were too hazardous for any previous Martian mission. Come hear about the “Seven Minutes of Terror” and the eight years of effort that went into the engineering behind the spacecraft.

Soumyo Dutta↗

Opportunities and Challenges for Machine Learning-Assisted Enzyme Engineering

Enzymes can be engineered at the level of their amino acid sequences to optimize key properties such as expression, stability, substrate range, and catalytic efficiency or even to unlock new catalytic activities not found in nature. Because the search space of possible proteins is vast, enzyme engineering usually involves discovering an enzyme starting point that has some level of the desired activity followed by directed evolution to improve its “fitness” for a desired application. Recently, machine learning (ML) has emerged as a powerful tool to complement this empirical process. ML models can contribute to (1) starting point discovery by functional annotation of known protein sequences or generating novel protein sequences with desired functions and (2) navigating protein fitness landscapes for fitness optimization by learning mappings between protein sequences and their associated fitness values. In this Outlook, we explain how ML complements enzyme engineering and discuss its future potential to unlock improved engineering outcomes.

60 APPLIED LIFE SCIENCES↗