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At least 55 records · Page 3

Active predation, phylogenetic diversity, and global prevalence of myxobacteria in wastewater treatment plants

The operation of modern wastewater treatment plants (WWTPs) is driven by activated sludge microbiota, a complex assemblage of trophically interacting microorganisms. Microbial predation is crucial to fundamental understanding of how biological interactions drive microbiome structuring and functioning of WWTPs. However, predatory bacteria have received little attention regarding their diversity, activity, and ecological function in activated sludge, limiting the exploitation of food web interactions for wastewater microbiome engineering. Here, by using rRNA-stable isotope probing of activated sludge microbiota with 13 C-labeled prey bacteria, we uncovered diverse as-yet-uncultivated putative predatory bacteria that actively incorporated 13 C-biomass. Myxobacteria, especially Haliangium and the mle1-27 clade, were found as the dominant active predators, refreshing conventional views based on a few predatory isolates of Bdellovibrionota from WWTPs. The identified predatory bacteria showed more selective predation on prey compared with the protists dominated by ciliates, providing in situ evidence for inter-domain predation behavior divergence in activated sludge. Putative predatory bacteria were tracked over a two-year microbiome monitoring effort at a local WWTP, revealing the predominance of Myxococcota (6.5 ± 1.3%) over Bdellovibrionota (1.0 ± 0.2%) lineages. Phylogenetic analysis unveiled highly diverse myxobacteria inhabiting activated sludge and suggested a habitat filtering effect in global WWTPs. Further mining of a global activated sludge microbiome dataset revealed the prevalence of Myxococcota (5.4 ± 0.1%) species and potential impacts of myxobacterial predation on process performance. Collectively, our findings provided unique insights into the predating activity, diversity, and prevalence of Myxococcota species in activated sludge, highlighting their links with wastewater treatment processes via trophic regulation of enteric and functional bacteria.

59 BASIC BIOLOGICAL SCIENCES↗

Pollen antigens and atmospheric circulation driven seasonal respiratory viral outbreak and its implication to the Covid-19 pandemic

Abstract The patterns of respiratory virus illness are expressed differently between temperate and tropical climates. Tropical outbreaks often peak in wet seasons. Temperate outbreaks typically peak during the winter. The prevailing causal hypotheses focus on sunlight, temperature and humidity variations. Yet no consistent factors have been identified to sufficiently explain seasonal virus emergence and decline at any latitude. Here we demonstrate close connections among global-scale atmospheric circulations, IgE antibody enhancement through seasonal pollen inhalation, and respiratory virus patterns at any populated latitude, with a focus on the US. Pollens emerge each Spring, and the renewed IgE titers in the population are argued to terminate each winter peak of respiratory illness. Globally circulated airborne viruses are postulated to subsequently deposit across the Southern US during lower zonal geostrophic winds each late Summer. This seasonally refreshed viral load is postulated to trigger a new influenza outbreak, once the existing IgE antibodies diminish to a critical value each Fall. Our study offers a new and consistent explanation for the seasonal diminishment of respiratory viral illnesses in temperate climates, the subdued seasonal signature in the tropics, the annually circulated virus phenotypes, and the northerly migration of influenza across the US every year. Our integrated geospatial and IgE hypothesis provides a new perspective for prediction, mitigation and prevention of the outbreak and spread of seasonal respiratory viruses including Covid-19 pandemic.

60 APPLIED LIFE SCIENCES↗

Extended X-ray absorption spectroscopy using an ultrashort pulse laboratory-scale laser-plasma accelerator

Laser-driven compact particle accelerators can provide ultrashort pulses of broadband X-rays, well suited for undertaking X-ray absorption spectroscopy measurements on a femtosecond timescale. Here the Extended X-ray Absorption Fine Structure (EXAFS) features of the K-edge of a copper sample have been observed over a 250 eV window in a single shot using a laser wakefield accelerator, providing information on both the electronic and ionic structure simultaneously. This capability will allow the investigation of ultrafast processes, and in particular, probing high-energy-density matter and physics far-from-equilibrium where the sample refresh rate is slow and shot number is limited. For example, states that replicate the tremendous pressures and temperatures of planetary bodies or the conditions inside nuclear fusion reactions. Using high-power lasers to pump these samples also has the advantage of being inherently synchronised to the laser-driven X-ray probe. A perspective on the additional strengths of a laboratory-based ultrafast X-ray absorption source is presented.

43 PARTICLE ACCELERATORS↗

Evaluating the WFIP2 updates to the HRRR model using scanning Doppler lidar measurements in the complex terrain of the Columbia River Basin

The wind-energy (WE) industry relies on numerical weather prediction (NWP) forecast models as foundational or base models for many purposes, including wind-resource assessment and wind-power forecasting. During the Second Wind Forecast Improvement Project (WFIP2) in the Columbia River Basin of Oregon and Washington, a significant effort was made to improve NWP forecasts through focused model development, to include experimental refinements to the High Resolution Rapid Refresh (HRRR) model physics and horizontal grid spacing. In this study, the performance of an experimental version of HRRR that includes these refinements is tested against a control version, which corresponds to that of the operational HRRR run by National Oceanic and Atmospheric Administration/National Centers for Environmental Protection at the outset of WFIP2. Furthermore, the effects of horizontal grid resolution were also tested by comparing wind forecasts from the HRRR (with 3-km grid spacing) with those from a finer-resolution HRRR nest with 750-m grid spacing. Model forecasts are validated against accurate wind-profile measurements by three scanning, pulsed Doppler lidars at sites separated by a total distance of 71 km. Model skill and improvements in model skill, attributable to physics refinements and improved horizontal grid resolution, varied by season, by site, and during periods of atmospheric phenomena relevant to WE. In general, model errors were the largest below 150 m above ground level (AGL). Experimental HRRR refinements tended to reduce the mean absolute error (MAE) and other error metrics for many conditions, but degradation in skill (increased MAE) was noted below 150 m AGL at the two lowest-elevation sites at night. Finer resolution was found to produce the most significant reductions in the error metrics.

17 WIND ENERGY↗

High pressure, high flow rate batch mixing apparatus for high throughput experiments

An automated, high pressure, high flow rate batch mixing apparatus has been designed and constructed for rapid, stable, and repeatable mixing of multiple gases and vapors. The apparatus operates as an intermittent batch mixer with cycles of topping off fresh mixture to maintain pressure in an accumulator tank until consumed in an experimental apparatus. At high duty cycles, the apparatus can also function at steady state. This style of mixing is suitable for experiments such as high repetition rate shock tubes and other devices with intermittent flow demands. It is compact and portable, facilitating use in locations such as synchrotron light sources. The entire apparatus is heated to permit the mixing of vapors from species with low volatilities. The apparatus is fully automated and runs for extended periods with the only intervention being to refresh reagent supplies. Here, the accuracy and repeatability of the apparatus were verified by periodic gas sampling and analysis with gas chromatography. Multi-component mixtures spanning a wide range of complexity, dilution, and volatility of constituents have been prepared. The compositions of the majority of the mixture were found to be stable over several filling cycles, repeatable, and with the proper calibration of set-point conditions, accurate. Challenges were encountered preparing a mixture from multi-component liquids, and potential solutions are discussed.

47 OTHER INSTRUMENTATION↗

Design and construction of a quantum matter synthesizer

The quantum matter synthesizer (QMS) is a new quantum simulation platform in which individual particles in a lattice can be resolved and re-arranged into arbitrary patterns. The ability to spatially manipulate ultracold atoms and control their tunneling and interactions at the single-particle level allows full control of a many-body quantum system. We present the design and characterization of the QMS, which integrates into a single ultra-stable apparatus a two-dimensional optical lattice, a moving optical tweezer array formed by a digital micromirror device, and site-resolved atomic imaging. We demonstrate excellent mechanical stability between the lattice and tweezer array with relative fluctuations below 10 nm, diffraction-limited imaging at a resolution of 655 nm, and high-speed real-time control of the tweezer array at a 2.52 kHz refresh rate, which will be adopted to realize fast rearrangement of atoms. The QMS also features new technologies and schemes, such as nanotextured anti-reflective windows and all-optical long-distance transport of atoms.

47 OTHER INSTRUMENTATION↗

Case study of a bore wind-ramp event from lidar measurements and HRRR simulations over ARM Southern Great Plains

The rapid change of wind speed and direction on 21 August 2017 is studied using Doppler lidar measurements at five sites of the Atmospheric Radiation Measurement (ARM) Southern Great Plains (SGP) facility in north-central Oklahoma. The Doppler lidar data were investigated along with meteorological variables such as temperature, humidity, and turbulence available from the large suite of instrumentation deployed at the SGP Central Facility (C1) during the Land-Atmosphere Feedback Experiment in August 2017. Lidar measurements at five sites, separated by 55-70 km, allowed us to document the development and evolution of the wind flow over the SGP area, examine synoptic conditions to understand the mechanism that leads to the ramp event, and estimate the ability of the High-Resolution Rapid Refresh model to reproduce this event. The flow feature in question is an atmospheric bore, a small-scale phenomenon that is challenging to represent in models, that was generated by a thunderstorm outflow northwest of the ARM SGP area. The small-scale nature of bores, its impact on power generation, and the modeling challenges associated with representing bores are discussed in this paper. In conclusion, the results also provide information about model errors between sites of different surface and vegetation types.

54 ENVIRONMENTAL SCIENCES↗

Overview of T and D–T results in JET with ITER-like wall

In 2021 JET exploited its unique capabilities to operate with T and D–T fuel with an ITER-like Be/W wall (JET-ILW). This second major JET D–T campaign (DTE2), after DTE1 in 1997, represented the culmination of a series of JET enhancements—new fusion diagnostics, new T injection capabilities, refurbishment of the T plant, increased auxiliary heating, in-vessel calibration of 14 MeV neutron yield monitors—as well as significant advances in plasma theory and modelling in the fusion community. DTE2 was complemented by a sequence of isotope physics campaigns encompassing operation in pure tritium at high T-NBI power. Carefully conducted for safe operation with tritium, the new T and D–T experiments used 1 kg of T (vs 100 g in DTE1), yielding the most fusion reactor relevant D–T plasmas to date and expanding our understanding of isotopes and D–T mixture physics. Furthermore, since the JET T and DTE2 campaigns occurred almost 25 years after the last major D–T tokamak experiment, it was also a strategic goal of the European fusion programme to refresh operational experience of a nuclear tokamak to prepare staff for ITER operation. The key physics results of the JET T and DTE2 experiments, carried out within the EUROfusion JET1 work package, are reported in this paper. Progress in the technological exploitation of JET D–T operations, development and validation of nuclear codes, neutronic tools and techniques for ITER operations carried out by EUROfusion (started within the Horizon 2020 Framework Programme and continuing under the Horizon Europe FP) are reported in (Litaudon et al Nucl. Fusion accepted), while JET experience on T and D–T operations is presented in (King et al Nucl. Fusion submitted).

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

100 Gb/s High Throughput Serial Protocol (HTSP) for data acquisition systems with interleaved streaming

Demands on Field-Programmable Gate Array (FPGA) data transport have been increasing over the years as frame sizes and refresh rates increase. As the bandwidths requirements increase the ability to implement data transport protocol layers using "soft" programmable logic becomes harder and start to require harden IP blocks implementation. To reduce the number of physical links and interconnects, it is common for data acquisition systems to require interleaving of streams on the same link (e.g. streaming data and streaming register access). Further, this paper presents a way to leverage existing FPGA harden IP blocks to achieve a robust, low latency 100 Gb/s point-to-point link with minimal programmable logic overhead geared towards the needs of data acquisition systems with interleaved streaming requirements.

47 OTHER INSTRUMENTATION↗

Hybrid biophysical systems for atmospheric CO 2 capture

Negative emissions technologies will be essential for limiting anthropogenic global temperature increases to 2 °C in the later years of the 21st century. Carbonic anhydrase (CA) metalloenzymes catalyze the otherwise slow conversion of CO 2 into carbonic acid (H 2 CO 3 ), suggesting their utility in the rapid hydration and downstream capture of dissolved CO 2 in aqueous media for a variety of CO 2 capture methods, such as thermal and pH swings and mineralization. The possibility of driving the rapid capture of CO 2 by catalyzing the CO 2 hydration bottleneck carries real potential for realizing efficient direct air capture (DAC) and direct ocean capture (DOC) systems. However, scaled application of CAs will be dependent on some way of economically sourcing the enzymes at volumes relevant to scaled DAC/DOC operations. In this perspective, we consider the prospect of catalyzing CO 2 hydration using a CA that is bound to the outer membrane of a cyanobacterial host, engineered constructs we call CyCAMs. Coupling extracellular CA expression to a marine, photosynthetic cyanobacterial host results in a CO 2 hydration catalyst that is continuously refreshed during the bacterial growth cycle, without the need for enzyme purification or harvesting steps. In the case of marine cyanobacteria, sunlight and the solutes native to seawater provide the respective energy, nutrients and sources of inorganic carbon essential to maintaining the bacterial population. We consider the potential impact of CA-mediated CO 2 hydration on a variety of DAC processes, with CO 2 mineralization explored as a primary use case.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Single-Cell Universal Logic-in-Memory Using 2T-nC FeRAM: An Area and Energy-Efficient Approach for Bulk Bitwise Computation

This work presents a novel approach to configure 2T-nC ferroelectric RAM (FeRAM) for performing single cell logic-in-memory operations, highlighting its advantages in energy-efficient computation over conventional DRAM-based approaches. Unlike conventional 1T-1C dynamic RAM (DRAM), which incurs refresh overhead, 2T-nC FeRAM offers a promising alternative as a non-volatile memory solution with low energy consumption. Our key findings include the potential of quasi-nondestructive readout (QNRO) sensing in 2T-nC FeRAM for logic-in-memory (LiM) applications, demonstrating its inherent capability to perform inverting logic without requiring external modifications, a feature absent in traditional 1T-1C DRAM. We successfully implement the MINORITY function within a single cell of 2T-nC FeRAM, enabling universal NAND and NOR logic, validated through SPICE simulations and experimental data. Additionally, the research investigates the feasibility of 3D integration with 2T-nC FeRAM, showing substantial improvements in storage and computational density, facilitating bulk-bitwise computation. Our evaluation of eight real-world, data-intensive applications reveals that 2T-nC FeRAM achieves 2× higher performance and 2.5× lower energy consumption compared to DRAM. Furthermore, the thermal stability of stacked 2T-nC FeRAM is validated, confirming its reliable operation when integrated on a compute die. These findings emphasize the advantages of 2T-nC FeRAM for LiM, offering superior performance and energy efficiency over conventional DRAM.

36 MATERIALS SCIENCE↗

Register-Like Storage Block Used as Histograms, Cluster Buffers, and Hough Transform Accumulators for HEP Trigger Systems

In high energy physics experiment trigger systems, block memories are utilized for various purposes, especially in binned searching algorithms. In these algorithms, the storages are demanded to perform like a large set of registers. The writing and reading operation must be performed in single clock cycle and once an event is processed, the memory must be globally reset. These demands can be fulfilled with registers but the cost of using registers for large memory is unaffordable. Another common requirement is the boundary coverage feature during reading process. Additionally, when a memory bin is addressed, the stored contents in the addressed bin and its neighboring bin must be output simultaneously. In this paper, a register-like block storage design scheme is described, which allows updating memory locations in single clock cycle, reading two adjacent bins, and effectively refreshing entire memory within a single clock. The implementation and test results are presented.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

Integration of New Technology Considering the Trade-Offs Between Operational Benefits and Risks: A Case Study of Dynamic Line Rating

Electric grid operators are adept at handling complexity and uncertainty. However, with increasing introduction of renewable generation, distributed energy resources, and more frequent severe weather events, operators will experience new workload and challenging decision scenarios. Here, this paper quantifies risks and benefits from an operator's perspective of introducing weather based forecast Dynamic Line Ratings (DLR) using variable wind conditions in addition to ambient temperature to relieve transmission congestion and facilitating more offshore wind (OSW). A concept of operations (CONOPS) applied to a forecast DLR implementation and its integration with OSW is defined. A method for evaluating tradeoffs of derating to make the rating more conservative but decreasing the benefit was developed and applied to a case study for two existing overhead transmission lines on Long Island, New York. The CONOPS uses historical day-ahead and hour-ahead High Resolution Rapid Refresh weather forecasts and weather station data to support planning and real-time operations. The analysis determines the risk of downgrades in real-time operational rating compared to the forecast and quantifies the frequency and severity of last-minute downgrades. The risk is compared against the benefits in increased capacity to provide insights on the additional amount of uncertainty DLR and OSW will add to the operator's workload.

17 WIND ENERGY↗

Enhancing Short-Range Weather Forecasts through Temporal Variation Encoding: A Multiperiod Embedding Approach

Machine learning (ML) techniques have emerged as promising approaches to improve regional weather forecast accuracy and reliability through data-driven methods. We propose a novel ML-based weather forecasting model, the Multiperiod Embed Net (MPENet). A key distinguishing feature of MPENet is its explicit utilization of the inherent cyclic nature in weather dynamics, unlike the autoregressive strategies commonly used in other ML weather forecasting approaches. Critical cyclic structures are identified via Fourier analyses of dynamic time series. Cyclicity in the convolutional representation is achieved by transforming one-dimensional time series of meteorological variables into two-dimensional tensors based on identified periods. This approach enables the model to leverage intrinsic weather patterns, enhancing regional forecast performance. To demonstrate the effectiveness of MPENet, we conduct a comparative analysis with Nvidia’s FourCastNet. Both models are trained on High-Resolution Rapid Refresh (HRRR) data from 2015 to 2022, over a 192 km × 192 km region in Tennessee. The comparisons are performed locally at two specific locations known to have different weather dynamics due to orographic effects: Crossville, on the relatively flat Cumberland Plateau with fewer topographic airflow disruptions, and Oak Ridge, in the ridge-and-valley region, where airflow is heavily influenced by surrounding valleys and mountains. Our results indicate that FourCastNet achieves strong accuracy at very short lead times, while MPENet maintains competitive skill and shows advantages in capturing temporal evolution over longer periods. Cross-correlation analyses of MPENet and FourCastNet predictions with the HRRR data suggest that encoding critical cyclicity into the network architecture leads to improvements in the forecasting skill.

Artificial intelligence↗

Improving Process Level Understanding of Boundary Layer Winds over the Northeast U.S. Shelf: The Third Wind Forecast Improvement Project (WFIP3)

The third Wind Forecast Improvement Project (WFIP3), a U.S. Department of Energy and National Oceanic and Atmospheric Administration sponsored investigation, sought to improve understanding of the physical phenomena in the atmosphere and ocean that dictate the structure and variability of wind and thermodynamic fields within the marine atmospheric boundary layer. WFIP3 focused on mesoscale and submesoscale flows -- including sea breezes, low-level jets, low-level clouds, and coastal storms -- and the ability of advanced numerical model parameterizations to represent them within fully coupled oceanic and atmospheric modeling systems and foundational weather forecast models. WFIP3 conducted a comprehensive 18-month observational study over the Northeast U.S. outer continental shelf, a high-use coastal zone, using a 3D multiscale sensor array to highly resolve the temporal, vertical, and horizontal structure of the coupled atmospheric and oceanic boundary layers. Multiple land-based study sites adjacent to the coastal ocean observed surface meteorology and vertical profiles of atmospheric properties via passive infrared and microwave radiometers, active lidars and radars, and radiosondes. At sea, an array of surface flux buoys and two vertical profiling lidar buoys observed both atmospheric and oceanic properties, augmented by land-based oceanographic radar systems and routine ship-based surveys. Intensive observations of the marine atmospheric boundary layer over the ocean was done from an air-sea interaction flux tower and extended deployments of a large autonomous barge platform. Numerous critical forecasting phenomena were observed that are being evaluated within regional coupled and uncoupled modeling systems, including the National Oceanic and Atmospheric Administration's foundational High-Resolution Rapid Refresh forecast model.

Kirincich, Anthony↗

Benchmarking Near-Surface Winds in the HRRR Analyses Using Multisource Observations over Complex Terrain in the Southeastern United States

Wind energy plays a crucial role in sustainable power generation, yet its full potential in the southeast United States (SEUS) remains underexplored. This study advances wind resource assessment in the SEUS using existing regional modeling and multisource observations. We find that terrain complexity, influenced by orography and forest canopy, significantly impacts the accuracy of modeled wind speed. While the High-Resolution Rapid Refresh (HRRR) model effectively simulates wind profiles over flat, nonforested terrain, larger errors are produced above forest canopies, and particularly for those stands in hilly and mountainous terrain where there are no observations aloft that could be used by HRRR’s data assimilation software. Seasonal variations, e.g., changes in the leaf area index, further complicate the relationship between terrain complexity and simulation errors. Here, our findings emphasize the critical need for comprehensive wind profile measurements, extending from below the canopy to height above the canopy and through the lower planetary boundary layer, to fully quantify model performance over and near forests, and for improving wind resource assessment, planning, and management. This study provides valuable insights not only for further model development but also for future field campaign deployment with the goal of further improving our understanding of wind resources in the region.

Complex terrain↗

Scale-Dependent Value of QPF for Real-Time Streamflow Forecasting

Incorporating rainfall forecasts into a real-time streamflow forecasting system extends the forecast lead time. Since quantitative precipitation forecasts (QPFs) are subject to substantial uncertainties, questions arise on the trade-off between the time horizon of the QPF and the accuracy of the streamflow forecasts. This study explores the problem systematically, exploring the uncertainties associated with QPFs and their hydrologic predictability. The focus is on scale dependence of the trade-off between the QPF time horizon, basin-scale, space-time scale of the QPF, and streamflow forecasting accuracy. To address this question, the study first performs a comprehensive independent evaluation of the QPFs at 140 U.S. Geological Survey (USGS) monitored basins with a wide range of spatial scales (~10 – 40,000 km 2 ) over the state of Iowa in the Midwestern United States. The study uses High-Resolution Rapid Refresh (HRRR) and Global Forecasting System (GFS) QPFs for short and medium-range forecasts, respectively. Using Multi-Radar Multi-Sensor (MRMS) quantitative precipitation estimate (QPE) as a reference, the results show that the rainfall-to-rainfall QPF errors are scale-dependent. The results from the hydrologic forecasting experiment show that both QPFs illustrate clear value for real-time streamflow forecasting at longer lead times in the short- to medium-range relative to the no-rain streamflow forecast. The value of QPFs for streamflow forecasting is particularly apparent for basin sizes below 1,000 km 2 . The space-time scale, or reference time t r ) (ratio of forecast lead time to basin travel time) ~ 1 depicts the largest streamflow forecasting skill with a systematic decrease in forecasting accuracy for t r > 1.

54 ENVIRONMENTAL SCIENCES↗

Observing Profiles of Derived Kinematic Field Quantities Using a Network of Profiling Sites

Abstract Observations of thermodynamic and kinematic parameters associated with derivatives of the thermodynamics and wind fields, namely, advection, vorticity, divergence, and deformation, can be obtained by applying Green’s theorem to a network of observing sites. The five nodes that comprise the Atmospheric Radiation Measurement (ARM) Southern Great Plains (SGP) profiling network, spaced 50–80 km apart, are used to obtain measurements of these parameters over a finite region. To demonstrate the applicability of this technique at this location, it is first applied to gridded model output from the High-Resolution Rapid Refresh (HRRR) numerical weather prediction model, using profiles from the locations of ARM network sites, so that values calculated from this method can be directly compared to finite difference calculations. Good agreement is found between both approaches as well as between the model and values calculated from the observations. Uncertainties for the observations are obtained via a Monte Carlo process in which the profiles are randomly perturbed in accordance with their known error characteristics. The existing size of the ARM network is well suited to capturing these parameters, with strong correlations to model values and smaller uncertainties than a more closely spaced network, yet it is small enough that it avoids the tendency for advection to go to zero over a large area.

54 ENVIRONMENTAL SCIENCES↗