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At least 55 records · Page 3

Understanding the structure and composition of recalcitrant oligosaccharides in hydrolysate using high-throughput biotin-based glycome profiling and mass spectrometry

Novel Immunological and Mass Spectrometry Methods for Comprehensive Analysis of Recalcitrant Oligosaccharides in AFEX Pretreated Corn Stover. Lignocellulosic biomass is a sustainable alternative to fossil fuel and is extensively used for developing bio-based technologies to produce products such as food, feed, fuel, and chemicals. The key to these technologies is to develop cost competitive processes to convert complex carbohydrates present in plant cell wall to simple sugars such as glucose, xylose, and arabinose. Since lignocellulosic biomass is highly recalcitrant, it must undergo a combination of thermochemical treatment such as Ammonia Fiber Expansion (AFEX), dilute acid (DA), Ionic Liquid (IL) and biological treatment such as enzyme hydrolysis and microbial fermentation to produce desired products. However, when using commercial fungal enzymes during hydrolysis, only 75–85% of the soluble sugars generated are monomeric sugars, while the remaining 15–25% are soluble recalcitrant oligosaccharides that cannot be easily utilized by microorganisms. Previously, we successfully separated and purified the soluble recalcitrant oligosaccharides using a combination of charcoal and celite-based separation followed by size exclusion chromatography and studies their inhibitory properties on enzymes. We discovered that the oligosaccharides with higher degree of polymerization (DP) containing methylated uronic acid substitutions were more recalcitrant towards commercial enzyme mixtures than lower DP and neutral oligosaccharides. Here, we report the use of several complementary techniques that include glycome profiling using plant biomass glycan specific monoclonal antibodies (mAbs) to characterize sugar linkages in plant cell walls and enzymatic hydrolysate, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) using structurally-informative diagnostic peaks offered by negative ion post-secondary decay spectra, gas chromatography followed by mass spectrometry (GC–MS) to characterize oligosaccharide sugar linkages with and without derivatization. Since oligosaccharides (DP 4–20) are small, it is challenging to mobilize these molecules for mAbs binding and characterization. To overcome this problem, we have applied a new biotin-coupling based oligosaccharide immobilization method that successfully tagged most of the low DP soluble oligosaccharides on to a micro-plate surface followed by specific linkage analysis using mAbs in a high-throughput system. This new approach will help develop more advanced versions of future high throughput glycome profiling methods that can be used to separate and characterize oligosaccharides present in biomarkers for diagnostic applications.

59 BASIC BIOLOGICAL SCIENCES↗

JANUS: Resilient and Adaptive Data Transmission for Enabling Timely and Efficient Cross-Facility Scientific Workflows

In modern science, the growing complexity of large-scale scientific projects has led to an increasing reliance on cross-facility scientific workflows, where resources and expertise from multiple institutions and geographic locations are leveraged to accelerate scientific discovery. These workflows often require transmitting huge amounts of scientific data through wide-area networks. Although high-speed networks like ESnet and transfer services such as Globus have improved data mobility, several challenges remain. The sheer volume of data can overwhelm network bandwidth, widely used transport protocols such as TCP suffer from inefficiencies due to retransmissions triggered by packet loss, and existing fault-tolerance mechanisms like erasure coding introduce substantial overhead. In this paper, we propose Janus, a resilient and adaptable data transmission approach designed for cross-facility scientific workflows. Unlike traditional TCP-based methods, Janus leverages UDP, integrates erasure coding for fault tolerance, and combines it with error-bounded lossy compression to reduce overhead. This novel design allows users to balance data transmission time and accuracy, optimizing transfer performance based on specific scientific requirements. Additionally, Janus dynamically adjusts erasure coding parameters in response to real-time network conditions, ensuring efficient data transfers even in fluctuating environments. We develop optimization models for determining ideal configurations and implement adaptive data transfer protocols to enhance reliability. Through extensive simulations and real-network experiments, we demonstrate that Janus significantly improves transfer efficiency while maintaining data fidelity.

Esaulov, Vladislav [Georgia State University, Atla↗

Optimization of Adjustable Drivetrain Assistance Mechanisms for Efficient Robotic Bipeds

Legged robots promise radical mobility for challenging environments, but must be made more energy efficient to be practical. Historically, legged robot design has required efficiency to be traded against versatility. Much energy is lost in actuators and transmissions because few actuation systems are capable of operating efficiently across the wide range of operating conditions (e.g. different joint speeds and torques) required for legged locomotion. We describe a drivetrain topology that overcomes many of these limitations. Our approach combines high-torque electromagnetic motors and low-loss transmissions with a tailored and adjustable set of joint-specific passive mechanisms called support elements, which modulate the energy flow between motors and joints to minimize the electrical energy consumed. We present an optimization-based design method that draws on available bipedal gait data to select optimal support element configurations and parameters. Simple adjustments may be made to support elements at certain joints to enable a wide variety of locomotion with high efficiency. We present results, specific to the 3D humanoid bipedal STEPPR robot, in which support elements are co-optimized across a library of several gaits, converging on a set of designs that predict an average reduction of electrical energy of more than 50% across a set of 15 gaits, with energy savings reaching as much as 85% for some gaits. Concepts were prototyped and tested on a bench testbed, validating the predicted energy savings. Support elements were implemented on STEPPR, and energy savings of more than 35% were demonstrated.

42 ENGINEERING↗

MSAT voice modulation considerations

The challenge for Mobile satellite (MSAT) voice services is to provide near toll quality voice to the user, while minimizing the power and bandwidth resources of the satellite. The options for MSAT voice can be put into one of two groups: Analog and Digital. Analog, nominally narrowband single sideband techniques, have a shown robustness to the fading and shadowing environment. Digital techniques, a combination of low rate vocoders and bandwidth efficient modems, show the promise of enhanced fidelity, as well as easier networking to the emerging digital world. The problems and tradeoffs to designers are many, especially in the digital case. Processor speed vs. cost and MET power requirements, channel coding, bandwidth efficiency vs. power efficiency etc. While the list looks daunting, in fact an acceptable solution is well within the technology. The objectives are reviewed that the MSAT voice service must meet, along with the options that are seen for the future.

Bossler, Dan↗

Development of an Electrostatic Precipitator to Remove Martian Atmospheric Dust from ISRU Gas Intakes During Planetary Exploration Missions

Manned exploration missions to Mars will need dependable in situ resource utilization (ISRU) for the production of oxygen and other commodities. One of these resources is the Martian atmosphere itself, which is composed of carbon dioxide (95.3%), nitrogen (2.7%), argon (1.6%), oxygen (0.13%), carbon monoxide (0.07%), and water vapor (0.03%), as well as other trace gases. However, the Martian atmosphere also contains relatively large amounts of dust, uploaded by frequent dust devils and high Winds. To make this gas usable for oxygen extraction in specialized chambers requires the removal of most of the dust. An electrostatic precipitator (ESP) system is an obvious choice. But with an atmospheric pressure just one-hundredth of Earth's, electrical breakdown at low voltages makes the implementation of the electrostatic precipitator technology very challenging. Ion mobility, drag forces, dust particle charging, and migration velocity are also affected because the low gas pressure results in molecular mean free paths that are approximately one hundred times longer than those at Earth .atmospheric pressure. We report here on our efforts to develop this technology at the Kennedy Space Center, using gases with approximately the same composition as the Martian atmosphere in a vacuum chamber at 9 mbars, the atmospheric pressure on Mars. We also present I-V curves and large particle charging data for various versions of wire-cylinder and rod-cylinder geometry ESPs. Preliminary results suggest that use of an ESP for dust collection on Mars may be feasible, but further testing with Martian dust simulant is required.

Clements, J. Sidney↗

Aram Chaos: a Long Lived Subsurface Aqueous Environment with Strong Water Resources Potential for Human Missions on Mars

Aram Chaos, Mars is a crater 280 kilometers in diameter with elevations circa. minus 2 to minus 3 kilometers below datum that provides a compelling landing site for future human explorers as it features multiple scientific regions of interest (ROI) paired with a rich extensible Resource ROI that features poly-hydrated sulfates [1]. The geologic history of Aram Chaos suggests several past episodes of groundwater recharge and infilling by liquid water, ice, and other materials [1-3]. The creation of the fractured region with no known terrestrial equivalent may have been caused by melting of deep ice reservoirs that triggered the collapse of terrain followed by catastrophic water outflows over the region. Aram Chaos is of particular scientific interest because it is hypothesized that the chaotic terrain may be the source of water that contributed to the creation of nearby valleys such as Ares Vallis flowing toward Chryse Planitia. The liquid water was likely sourced as groundwater and therefore represents water derived from a protected subsurface environment making it a compelling astrobiological site [2]. The past history of water is also represented by high concentrations of hematite, Fe-oxyhydroxides, mono-hydrated and poly-hydrated sulfates [1, 2]. Poly-hydrated sulfates are likely to contain abundant water that evolves at temperatures below 500 degrees Centigrade thus conferring Aram Chaos a potentially high value for early in-situ resource utilization (ISRU) [4]. The geologic history also calls for future prospecting of deep ice deposits and possibly liquid water via deep drilling. The most recent stratigraphic units in the central part of Aram Chaos are not fractured, and are part of a dome-shaped formation that features bright, poorly-consolidated material that contains both hydrated sulfates and ferric oxides according to OMEGA (Observatoire pour la Minéralogie, l'Eau, les Glaces et l'Activité) data [5]. These surface material characteristics are preliminary indications of their potential use in civil engineering activities that involve regolith moving and hauling, while further study is needed to assess traverse-ability challenges. The widespread distribution of sulfates is also of interest as a resource for the use of sulfur as a binding compound in regolith-based concrete for constructions. The terrain depressions caused by the rock fracturing events may challenge surface mobility but also suggest the possibility of using such natural features for additional shielding from space radiation and as emplacement of nuclear surface power reactors for the same reason. The high concentration of hematite (up to 16 percent) in some of the smoother recent terrains of the central part of Aram Chaos [2] is a favorable attribute for metal extraction ISRU to create iron-based feedstock for in-situ fabrication of replacement parts or their repairs. Preliminary data on Aram Chaos indicate that it offers a combination of many critical criteria for human missions to the surface of Mars: equatorial region at low Mars Orbiter Laser Altimeter (MOLA), evidence of hydrated minerals over large areas and at high concentrations tied to historic evidence of liquid water over long periods.

Sibille, L.↗

UAV Research at NASA Langley: Towards Safe, Reliable, and Autonomous Operations

Unmanned Aerial Vehicles (UAV) are fundamental components in several aspects of research at NASA Langley, such as flight dynamics, mission-driven airframe design, airspace integration demonstrations, atmospheric science projects, and more. In particular, NASA Langley Research Center (Langley) is using UAVs to develop and demonstrate innovative capabilities that meet the autonomy and robotics challenges that are anticipated in science, space exploration, and aeronautics. These capabilities will enable new NASA missions such as asteroid rendezvous and retrieval (ARRM), Mars exploration, in-situ resource utilization (ISRU), pollution measurements in historically inaccessible areas, and the integration of UAVs into our everyday lives all missions of increasing complexity, distance, pace, and/or accessibility. Building on decades of NASA experience and success in the design, fabrication, and integration of robust and reliable automated systems for space and aeronautics, Langley Autonomy Incubator seeks to bridge the gap between automation and autonomy by enabling safe autonomous operations via onboard sensing and perception systems in both data-rich and data-deprived environments. The Autonomy Incubator is focused on the challenge of mobility and manipulation in dynamic and unstructured environments by integrating technologies such as computer vision, visual odometry, real-time mapping, path planning, object detection and avoidance, object classification, adaptive control, sensor fusion, machine learning, and natural human-machine teaming. These technologies are implemented in an architectural framework developed in-house for easy integration and interoperability of cutting-edge hardware and software.

Davila, Carlos G.↗

Urban Air Mobility Electric Motor Winding Insulation Reliability: Challenges in the Design and Qualification of High Reliability Electric Motors and NASA’s Research Plan

This work was motivated by the needs of the emerging Urban Air Mobility (UAM) concept. Guided by analyses of concept vehicles, electric motor winding insulation reliability was identified as a subsystem limiting the vehicle reliability. This paper covers NASA’s understanding of the current state-of-the-art for electric motor winding insulation reliability, life modeling and qualification testing in the context of UAM. The needed improvements to the accepted practice for qualification of high reliability UAM motors are highlighted. NASA’s Revolutionary Vertical Lift Technologies Project strives to develop new testing methods to support the rating and qualification of UAM motor winding insulation. We describe assumptions used to frame a research approach, and we outline a NASA RVLT research plan for motor winding insulation.

Thomas Tallerico↗

Challenges with Deploying and Integrating Environmental Control and Life Support Functions in a Lunar Architecture with High Degrees of Mobility

Visions of lunar outposts often depict a collection of fixed elements such as pressurized habitats, in and around which human inhabitants spend the large majority of their surface stay time. In such an outpost, an efficient deployment of environmental control and life support equipment can be achieved by centralizing certain functions within one or a minimum number of habitable elements and relying on the exchange of gases and liquids between elements via atmosphere ventilation and plumbed interfaces. However, a rigidly fixed outpost can constrain the degree to which the total lunar landscape can be explored. The capability to enable widespread access across the landscape makes a lunar architecture with a high degree of surface mobility attractive. Such mobility presents unique challenges to the efficient deployment of environmental control and life support functions in multiple elements that may for long periods of time be operated independently. This paper describes some of those anticipated challenges.

Bagdigian, Robert M.↗

Analysis of Electrical Grid Capacity in Major U.S. Metropolitan Areas for Urban Air Mobility

A wide range of challenges must be overcome to conduct Urban Air Mobility (UAM) operations at scale with electric vertical takeoff and landing aircraft as envisioned. This paper documents an analysis of one potential infrastructure challenge for UAM: available electrical grid power capacity. The success of UAM depends on the availability of electricity, which will be increasingly impacted by the proliferation of ground electric vehicles (EVs) over time. Under the worst-case assumption that each U.S. Metropolitan Statistical Area (MSA) can only access and utilize the electricity generated by power plants within its boundaries, it is estimated that the maximum number of UAM aircraft that can charge simultaneously in the 25 most populous MSAs will decrease by about 61% from about 129,000 in the year 2021 to about 51,000 in the year 2050, due to the projected increase in ground EVs. Also, it is estimated that the number of these MSAs that may lack any available electrical grid power capacity for UAM operations will increase from zero MSAs in 2021 to eleven MSAs in 2050—including three MSAs that prospective UAM operators have announced as target markets (Los Angeles, Miami, and Orlando)—unless electricity demand for other purposes is reduced.

electrical grid↗

Analysis of Electrical Grid Capacity in Major U.S. Metropolitan Areas for Urban Air Mobility

A wide range of challenges must be overcome to conduct Urban Air Mobility (UAM) operations at scale with electric vertical takeoff and landing aircraft as envisioned. This paper documents an analysis of one potential infrastructure challenge for UAM: available electrical grid power capacity. The success of UAM depends on the availability of electricity, which will be increasingly impacted by the proliferation of ground electric vehicles (EVs) over time. Under the worst-case assumption that each U.S. Metropolitan Statistical Area (MSA) can only access and utilize the electricity generated by power plants within its boundaries, it is estimated that the maximum number of UAM aircraft that can charge simultaneously in the 25 most populous MSAs will decrease by about 61% from about 129,000 in the year 2021 to about 51,000 in the year 2050, due to the projected increase in ground EVs. Also, it is estimated that the number of these MSAs that may lack any available electrical grid power capacity for UAM operations will increase from zero MSAs in 2021 to eleven MSAs in 2050—including three MSAs that prospective UAM operators have announced as target markets (Los Angeles, Miami, and Orlando)—unless electricity demand for other purposes is reduced.

electrical grid↗

Analysis of Electrical Grid Capacity by Interconnection for Urban Air Mobility

A wide range of challenges must be overcome to conduct Urban Air Mobility (UAM)operations at scale with electric vertical take-off and landing aircraft as envisioned. This paper documents an analysis of one potential infrastructure challenge for UAM: available electrical grid power capacity. The success of UAM depends on the availability of electricity, which will be increasingly impacted by the proliferation of ground electric vehicles(EVs) over time. Under the best-case assumption that electricity can be transmitted and distributed as needed within each interconnection (i.e., electrical grid), it is estimated that the maximum number of UAM aircraft that can charge simultaneously in the continental United States will decrease by 20% from about 595 thousand in the year 2021 to about 475 thousand in the year 2050due to the projected increase in ground EVs.

electrical grid↗

Analysis of Electrical Grid Capacity by Interconnection for Urban Air Mobility

A wide range of challenges must be overcome to conduct Urban Air Mobility (UAM)operations at scale with electric vertical take-off and landing aircraft as envisioned. This paper documents an analysis of one potential infrastructure challenge for UAM: available electrical grid power capacity. The success of UAM depends on the availability of electricity, which will be increasingly impacted by the proliferation of ground electric vehicles(EVs) over time. Under the best-case assumption that electricity can be transmitted and distributed as needed within each interconnection (i.e., electrical grid), it is estimated that the maximum number of UAM aircraft that can charge simultaneously in the continental United States will decrease by 20% from about 595 thousand in the year 2021 to about 475 thousand in the year 2050due to the projected increase in ground EVs.

electrical grid↗

Rovers for intelligent, agile traverse of challenging terrain

Planetary surface mobility has to date been limited to benign locations. If rover systems could be developed for more challenging terrain, e.g., sloped and irregularly feathered areas, then planetary science opportunities would be greatly expanded.

rover terrain surface mobility↗

The Challenge of Planning and Execution for Spacecraft Mobile Robots

The need for spacecraft mobile robots continues to grow. These robots offer the potential to increase the capability, productivity, and duration of space missions while decreasing mission risk and cost. Spacecraft Mobile Robots (SMRs) can serve a number of functions inside and outside of spacecraft from simpler tasks, such as performing visual diagnostics and crew support, to more complex tasks, such as performing maintenance and in-situ construction. One of the predominant challenges to deploying SMRs is to reduce the need for direct operator interaction. Teleoperation is often not practical due to the communication latencies incurred because of the distances involved and in many cases a crewmember would directly perform a task rather than teleoperate a robot to do it. By integrating a mixed-initiative constraint-based planner with an executive that supports adjustably autonomous control, we intend to demonstrate the feasibility of autonomous SMRs by deploying one inside the International Space Station (ISS) and demonstrate in simulation one that operates outside of the ISS. This paper discusses the progress made at NASA towards this end, the challenges ahead, and concludes with an invitation to the research community to participate.

Dorais, Gregory A.↗

Exploring the Utility-Privacy Trade-Off: Impacts of Semantic and Visit Types Ambiguities on Human Mobility Simulation

Humans are in perpetual movement, constantly traversing buildings, cities, waters, oceans, and countries. Mobility stands out as a major driving force shaping our modern societies. Capturing and explaining human behavior in a world of eight billion distinct mobility agendas is a complex challenge. With the rise of interconnected devices and platforms, such as smartphones, wearables, and point-of-interest data, largescale behavioral data has become more accessible, enabling rich insights into mobility patterns. However, the widespread availability of such data introduces significant ethical challenges. Detailed mobility data can inadvertently reveal sensitive personal information, including individuals' locations, habits, social interactions, and even political or religious affiliations. Beyond privacy breaches, the ethical implications of uncovering and potentially manipulating underlying behavioral patterns demand attention. Striking a balance between the utility of mobility models and the protection of individual privacy is therefore paramount. This paper explores the utility-privacy trade-offs in human mobility modeling, focusing on the impacts of introducing semantic and visit type ambiguities. By systematically examining how these ambiguities affect the fidelity of simulated trajectories and privacy risks, we provide a framework for evaluating ethical and privacy-conscious modeling practices. Our findings emphasize the need for methods that safeguard privacy without undermining the usefulness of mobility models, contributing to the responsible advancement of mobility science in alignment with ethical standards and societal expectations.

Amichi, Licia [ORNL] (ORCID:0000000177631394)↗

Efficient proactive vehicle relocation for on-demand mobility service with recurrent neural networks

One major challenge for on-demand mobility service (OMS) providers is to seamlessly match empty vehicles with trip requests so that the total vacant mileage is minimized. In this work, we develop an innovative data-driven approach for devising efficient vehicle relocation policy for OMS that (1) proactively relocates vehicles before the demand is observed and (2) reduces the inequality among drivers' income so that the proactive relocation policy is fair and is likely to be followed by drivers. Our approach represents the fusion of optimization and machine learning methods, which comprises three steps: First, we formulate the optimal proactive relocation as an optimal/stable matching problems and solve for global optimal solutions based on historical data. Second, the optimal solutions are then grouped and fed to train the deep learning models which consist of fully connected layers and long short-term memory networks. Low rank approximation is introduced to reduce the model complexity and improve the training performances. Finally, we use the trained model to predict the relocation policy which can be implemented in real time. We conduct comprehensive numerical experiments and sensitivity analyses to demonstrate the performances of the proposed method using New York City taxi data. Here, the results suggest that our method will reduce empty mileage per trip by 54-70% under optimal matching strategy, and a 25-32% reduction can also be achieved by following stable matching strategy. We also validate that the predicted relocation policies are robust in the presence of uncertain passenger demand level and passenger trip-requesting behavior.

33 ADVANCED PROPULSION SYSTEMS↗