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The Cell Utilized Partitioning Model as a Predictive Tool for Optimizing Counter-Current Chromatography Processes

Counter-current chromatography (CCC) is capable of unique elution modes that isolate analytes using the movement of the stationary phase in addition to moving the mobile phase. These modes include elution-extrusion CCC (EECCC) and dual-mode CCC (DM CCC) that are not possible in traditional solid-liquid chromatography systems. Although EECCC and DM CCC are widely used to recover highly retained components, to our knowledge, optimizing the elution process in these modes with predictive models has not been reported. To address this gap, we developed a predictive model for CCC dubbed the Cell Utilized Partitioning (CUP) model. The CUP model accurately predicts the effluents of multicomponent separations in EECCC and DM CCC modes when compared to experimental data. Furthermore, CUP model simulations were extended to investigate the influence of operating and intrinsic parameters on the yield and productivity, and to compare the separation performances of EECCC and DM CCC in various conditions. The results demonstrate that low distribution constants, usually a KD less than 1, and a selectivity > 1.3, under specific flowrate ranges, increase both productivity and yield. From these results, generalized optimization and scaleup guidelines are proposed that can apply to research settings and to industrial processes to maximize preparative CCC performance.

BIOMASS FUELS,INORGANIC, ORGANIC, PHYSICAL, AND AN↗

On principles, methods and recent advances in studies towards a GPS-based control system for geodesy and geodynamics

Although Very Long Baseline Interferometry (VLBI) and Satellite Laser Ranging (SLR) are becoming increasingly important tools for geodynamic studies, their future role may well be fulfilled by using alternative techniques such as those utilizing the signals from the Global Positioning System (GPS). GPS, without the full implementation of the system, already offers a favorable combination of cost and accuracy and has consistently demonstrated the capability to provide high precision densification control in the regional and local areas of the VLBI and SLR networks. This report reviews VLBI and SLR vis-a-vis GPS and outlines the capabilities and limitations of each technique and how their complementary application can be of benefit to geodetic and geodynamic operations. It demonstrates, albeit with a limited data set, that dual-frequency GPS observations and interferometric type analysis techniques make possible the modelling of the GPS orbits for several days with an accuracy of a few meters. The use of VLBI or SLR sites as fiducial stations together with refinements in the orbit determination procedures can greatly reduce the systematic errors in the GPS satellite orbits used to compute the positions of non-fiducial locations. In general, repeatability and comparison with VLBI of the GPS determined locations are of the order of between 2 parts in 10 to the 7th power and 5 parts in 10 to the 8th power for baseline lengths less than 2000 km. This report is mainly a synthesis of problems, assumptions, methods and recent advances in the studies towards the establishment of a GPS-based system for geodesy and geodynamics and is one phase in the continuing effort for the development of such a system. To some, including the author, it seems reasonable to expect within the next few years that more evidence will show GPS to be as a powerful and reliable a tool as mobile VLBI and SLR are today, but largely more economical.

Delikaraoglou, Demitris↗

EMU Lessons Learned Database

As manned space exploration takes on the task of traveling beyond low Earth orbit, many problems arise that must be solved in order to make the journey possible. One major task is protecting humans from the harsh space environment. The current method of protecting astronauts during Extravehicular Activity (EVA) is through use of the specially designed Extravehicular Mobility Unit (EMU). As more rigorous EVA conditions need to be endured at new destinations, the suit will need to be tailored and improved in order to accommodate the astronaut. The Objective behind the EMU Lessons Learned Database(LLD) is to be able to create a tool which will assist in the development of next-generation EMUs, along with maintenance and improvement of the current EMU, by compiling data from Failure Investigation and Analysis Reports (FIARs) which have information on past suit failures. FIARs use a system of codes that give more information on the aspects of the failure, but if one is unfamiliar with the EMU they will be unable to decipher the information. A goal of the EMU LLD is to not only compile the information, but to present it in a user-friendly, organized, searchable database accessible to all familiarity levels with the EMU; both newcomers and veterans alike. The EMU LLD originally started as an Excel database, which allowed easy navigation and analysis of the data through pivot charts. Creating an entry requires access to the Problem Reporting And Corrective Action database (PRACA), which contains the original FIAR data for all hardware. FIAR data are then transferred to, defined, and formatted in the LLD. Work is being done to create a web-based version of the LLD in order to increase accessibility to all of Johnson Space Center (JSC), which includes converting entries from Excel to the HTML format. FIARs related to the EMU have been completed in the Excel version, and now focus has shifted to expanding FIAR data in the LLD to include EVA tools and support hardware such as the Pistol Grip Tool (PGT) and the Battery Charger Module (BCM), while adding any recently closed EMU-related FIARs.

Matthews, Kevin M., Jr.↗

Assistive Detect and Avoid Technology in Urban Air Mobility Environments

The use of Assistive Detect and Avoid (Assistive DAA or ADAA) technology in Urban Air Mobility (UAM) environments poses potential benefits as well as challenges. Assistive DAA refers to the leveraged use of DAA technology, originally developed to replace see-and-avoid capabilities for remotely piloted aircraft, in onboard-piloted aircraft to augment (rather than replace) pilots’ see-and-avoid abilities and thus enhance the safety and efficiency of visual flight operations. ADAA is anticipated to be especially safety-enhancing in airspace where traffic density is high or traditional air traffic services are limited, such as in future UAM environments. ADAA may also enable higher-tempo UAM operations than with only see-and-avoid capabilities, while still maintaining acceptable levels of safety. UAM concepts under development by the FAA, NASA, and industry focus on operations moving people and cargo in urban and suburban areas using innovative technologies, operations, and aircraft, including electric vertical takeoff and landing (eVTOL) aircraft. Researchers at NASA Langley Research Center, in collaboration with FAA researchers at the William J. Hughes Technical Center in Atlantic City, NJ, have conducted a series of medium-fidelity, human-in-the-loop research simulations of potential future UAM operations and concepts in both Class C and Class B airspace environments. These simulations have included use of a Langley-developed ADAA research tool called DANTi, which enables configurable ADAA displays to be presented to pilots of simulated eVTOL aircraft participating in higher-density and higher-tempo UAM operations. Experience and observations made during testing of the NASA-developed DANTi ADAA capability in the UAM NFLITE simulation environment will be reported in this paper together with a discussion of airspace integration and regulatory topics.

Detect and Avoid↗

Assistive Detect and Avoid Technology in Urban Air Mobility Environments

The use of Assistive Detect and Avoid (Assistive DAA or ADAA) technology in Urban Air Mobility (UAM) environments poses potential benefits as well as challenges. Assistive DAA refers to the leveraged use of DAA technology, originally developed to replace see-and-avoid capabilities for remotely piloted aircraft, in onboard-piloted aircraft to augment (rather than replace) pilots’ see-and-avoid abilities and thus enhance the safety and efficiency of visual flight operations. ADAA is anticipated to be especially safety-enhancing in airspace where traffic density is high or traditional air traffic services are limited, such as in future UAM environments. ADAA may also enable higher-tempo UAM operations than with only see-and-avoid capabilities, while still maintaining acceptable levels of safety. UAM concepts under development by the FAA, NASA, and industry focus on operations moving people and cargo in urban and suburban areas using innovative technologies, operations, and aircraft, including electric vertical takeoff and landing (eVTOL) aircraft. Researchers at NASA Langley Research Center, in collaboration with FAA researchers at the William J. Hughes Technical Center in Atlantic City, NJ, have conducted a series of medium-fidelity, human-in-the-loop research simulations of potential future UAM operations and concepts in both Class C and Class B airspace environments. These simulations have included use of a Langley-developed ADAA research tool called DANTi, which enables configurable ADAA displays to be presented to pilots of simulated eVTOL aircraft participating in higher-density and higher-tempo UAM operations. Experience and observations made during testing of the NASA-developed DANTi ADAA capability in the UAM NFLITE simulation environment will be reported in this paper together with a discussion of airspace integration and regulatory topics.

Detect and Avoid↗

Benchmarking the PCMCI Causal Discovery Algorithm for Spatiotemporal Systems

Causal discovery algorithms construct hypothesized causal graphs that depict causal dependencies among variables in observational data. While powerful, the accuracy of these algorithms is highly sensitive to the underlying dynamics of the system in ways that have not been fully characterized in the literature. In this report, we benchmark the PCMCI causal discovery algorithm in its application to gridded spatiotemporal systems. Effectively computing grid-level causal graphs on large grids will enable analysis of the causal impacts of transient and mobile spatial phenomena in large systems, such as the Earth’s climate. We evaluate the performance of PCMCI with a set of structural causal models, using simulated spatial vector autoregressive processes in one- and two-dimensions. We develop computational and analytical tools for characterizing these processes and their associated causal graphs. Our findings suggest that direct application of PCMCI is not suitable for the analysis of dynamical spatiotemporal gridded systems, such as climatological data, without significant preprocessing and downscaling of the data. PCMCI requires unrealistic sample sizes to achieve acceptable performance on even modestly sized problems and suffers from a notable curse of dimensionality. This work suggests that, even under generous structural assumptions, significant additional algorithmic improvements are needed before causal discovery algorithms can be reliably applied to grid-level outputs of earth system models.

54 ENVIRONMENTAL SCIENCES↗

Simulating Fleet Noise for Notional UAM Vehicles and Operations in New York

This paper presents the results of systems-level simulations using Metrosim that were conducted for notional Urban Air Mobility (UAM)-style vehicles analyzed for two different scenarios for New York (NY). UAM is an aviation industry term for passenger or cargo-carrying air transportation services, which are often automated, operating in an urban/city environment. UAM-style vehicles are expected to use vertical takeoff and landing with fixed wing cruise flight. Metrosim is a metroplex-wide route and airport planning tool that can also be used in standalone mode as a simulation tool. The scenarios described and reported in this paper were used to evaluate a fleet noise prediction capability for this tool. The work was a collaborative effort between the National Aeronautics and Space Administration (NASA), Intelligent Automation, Inc (IAI), and the Port Authority of New York and New Jersey (PANYNJ). One scenario was designed to represent an expanded air-taxi operation from existing helipads around Manhattan to the major New York airports. The other case represented a farther term vision case with commuters using personal air vehicles to hub locations just outside New York, with an air-taxi service running frequent connector trips to a few key locations inside Manhattan. For both scenarios, the trajectories created for the entire fleet were passed to the Aircraft Environmental Design Tool (AEDT) to generate Day-Night Level (DNL) noise contours for inspection. Without data for actual UAM vehicles available, surrogate AEDT empirical Noise-Power-Distance (NPD) tables used a similar sized current day helicopter as the Baseline, and a version of that same data linearly scaled as a first guess at possible UAM noise data. Details are provided for each of the two scenario configurations, and the output noise contours are presented for the Baseline and reduced noise DNL cases.

Glaab, Patricia↗

A Summary of NASA Rotary Wing Research: Circa 2008–2018

The general public may not know that the first “A” in NASA stands for Aeronautics. If they do know, they will very likely be surprised that in addition to airplanes, the “A” includes research in helicopters, tiltrotors, and other vehicles adorned with rotors. There is, arguably, no subsonic air vehicle more difficult to accurately analyze than a vehicle with lift-producing rotors. No wonder that NASA has conducted rotary wing research since the days of the NACA and has partnered, since 1965, with the U.S. Army in order to overcome some of the most challenging obstacles to understanding the behavior of these vehicles. Since 2006, NASA rotary wing research has been performed under several different project names [Gorton et al., 2015]: Subsonic Rotary Wing (SRW) (2006–2012), Rotary Wing (RW) (2012–2014), and Revolutionary Vertical Lift Technology (RVLT) (2014–present). In 2009, the SRW Project published a report that assessed the status of NASA rotorcraft research; in particular, the predictive capability of NASA rotorcraft tools was addressed for a number of technical disciplines. A brief history of NASA rotorcraft research through 2009 was also provided [Yamauchi and Young, 2009]. Gorton et al. [2015] describes the system studies during 2009–2011 that informed the SRW/RW/RVLT project investment prioritization and organization. The authors also provided the status of research in the RW Project in engines, drive systems, aeromechanics, and impact dynamics as related to structural dynamics of vertical lift vehicles. Since 2009, the focus of research has shifted from large civil VTOL transports, to environmentally clean aircraft, to electrified VTOL aircraft for the urban air mobility (UAM) market. The changing focus of rotorcraft research has been a reflection of the evolving strategic direction of the NASA Aeronautics Research Mission Directorate (ARMD). By 2014, the project had been renamed the Revolutionary Vertical Lift Technology Project. In response to the 2014 NASA Strategic Plan, ARMD developed six Strategic Thrusts. Strategic Thrust 3B was defined as the “Ultra-Efficient Commercial Vehicles—Vertical Lift Aircraft.” Hochstetler et al. [2017] uses Thrust 3B as an example for developing metrics usable by ARMD to measure the effectiveness of each of the Strategic Thrusts. The authors provide near-, mid-, and long-term outcomes for Thrust 3B with corresponding benefits and capabilities. The importance of VTOL research, especially with the rapidly expanding UAM market, eventually resulted in a new Strategic Thrust (to begin in 2020): Thrust 4—Safe, Quiet, and Affordable Vertical Lift Air Vehicles. The underlying rotary wing analysis tools used by NASA are still applicable to traditional rotorcraft and have been expanded in capability to accommodate the growing number of VTOL configurations designed for UAM. The top-level goal of the RVLT Project remains unchanged since 2006: Develop and validate tools, technologies and concepts to overcome key barriers for vertical lift vehicles. In 2019, NASA rotary wing/VTOL research has never been more important for supporting new aircraft and advancements in technology. 2 A decade is a reasonable interval to pause and take stock of progress and accomplishments. In 10 years, digital technology has propelled progress in computational efficiency by orders of magnitude and expanded capabilities in measurement techniques. The purpose of this report is to provide a compilation of the NASA rotary wing research from ~2008 to ~2018. Brief summaries of publications from NASA, NASA-funded, and NASA-supported research are provided in 12 chapters: Acoustics, Aeromechanics, Computational Fluid Dynamics (External Flow), Experimental Methods, Flight Dynamics and Control, Drive Systems, Engines, Crashworthiness, Icing, Structures and Materials, Conceptual Design and System Analysis, and Mars Helicopter. We hope this report serves as a useful reference for future NASA vertical lift researchers.

Yamauchi, Gloria K.↗

Narrowband and wideband characterisation of satellite mobile/PCN channel

This paper presents models characterizing satellite mobile channel. Statistical narrowband models based on the CSER high elevation angle channel measurement campaign are reported. Such models are understood to be useful for communication system simulations. It has been shown from the modelling results that for the mobile satellite links at high elevation angles line-of-sight (LOS) signal is available most of the time, even under the heavy shadowing conditions. Wideband measurement campaign which CSER is about to undertake, and subsequently the modelling approach to be adopted is also discussed. It is noted that a wideband channel model is expected to provide a useful tool in investigating CDMA applications.

Butt, G.↗

Expedition UT-GOM2-2 Methods

Methods used during the University of Texas (UT) Deepwater Hydrate Coring Expedition (UT-GOM2-2) include work done onboard the Helix Q4000 in the offshore Gulf of America (Gulf of Mexico), herein “the Gulf”, “dockside” in Salt Lake City, Utah, and some shore-based work in individual laboratories. The goal of this report is two-fold: to provide enough detail on the methods so they can be repeated by others; and to provide a reference document for the team to enhance cross-disciplinary understanding and knowledge. Methods include drilling operations, depth references and depth modification, downhole tool deployment, coring tool performance assessment, core processing, lithostratigraphy, biostratigraphy, as well as physical properties, including core logging and imaging, rock magnetism, dissolved methane concentration, hydrate saturation, microbiology, and geochemistry. An extensive amount of operational work and planning was required before mobilization of the expedition to permit, build mobile labs, and test downhole tools for deepwater drilling.

03 NATURAL GAS↗

Spherical Coordinate Systems for Streamlining Suited Mobility Analysis

When describing human motion, biomechanists generally report joint angles in terms of Euler angle rotation sequences. However, there are known limitations in using this method to describe complex motions such as the shoulder joint during a baseball pitch. Euler angle notation uses a series of three rotations about an axis where each rotation is dependent upon the preceding rotation. As such, the Euler angles need to be regarded as a set to get accurate angle information. Unfortunately, it is often difficult to visualize and understand these complex motion representations. One of our key functions is to help design engineers understand how a human will perform with new designs and all too often traditional use of Euler rotations becomes as much of a hindrance as a help. It is believed that using a spherical coordinate system will allow ABF personnel to more quickly and easily transmit important mobility data to engineers, in a format that is readily understandable and directly translatable to their design efforts. Objectives: The goal of this project is to establish new analysis and visualization techniques to aid in the examination and comprehension of complex motions. Methods: This project consisted of a series of small sub‐projects, meant to validate and verify the method before it was implemented in the ABF's data analysis practices. The first stage was a proof of concept, where a mechanical test rig was built and instrumented with an inclinometer, so that its angle from horizontal was known. The test rig was tracked in 3D using an optical motion capture system, and its position and orientation were reported in both Euler and spherical reference systems. The rig was meant to simulate flexion/extension, transverse rotation and abduction/adduction of the human shoulder, but without the variability inherent in human motion. In the second phase of the project, the ABF estimated the error inherent in a spherical coordinate system, and evaluated how this error would vary within the reference frame. This stage also involved expanding a kinematic model of the shoulder, to include the torso, knees, ankle, elbows, wrists and neck. Part of this update included adding a representation of 'roll' about an axis, for upper arm and lower leg rotations. The third stage of the project involved creating visualization methods to assist in interpreting motion in a spherical frame. This visualization method will be incorporated in a tool to evaluate a database of suited mobility data, which is currently in development.

Benson, Elizabeth↗

Application of Manufacturing Quality Management Principles to PV System Installations

To help SETO/DOE achieve its goals, the IBTS team proposed a project addressing system reliability by improving installation standards and quality management. The proposed approach was designed to help achieve measurable reductions in installation defect density and improvements in the performance of PV systems by optimizing design and installation of residential and commercial PV systems. This approach addressed the soft costs associated with installations and quality management. The project demonstrated improved system reliability and reduced PV system installation costs. The software developed improved operations, decreased risk, and increased the overall value of PV systems across their lifecycle. The project used several data collection methods, including extensive industry surveys, face-to-face high-level interviews at industry conferences, stakeholder teleconferences, and in-depth interviews conducted by IBTS staff. Results from the research found the industry needs a uniform assessment method for national providers to be more efficient; the software should support both code officials and installers; most industry stakeholders would find value in a centralized software system that allows them to collect, report, and review information on in-process and completed solar installations; and mobile solutions that bridge existing knowledge gaps with inspectors and integrate with existing methodologies (such as permitting software) are of great value. The software solution developed is web-based, allowing for national access, and is built on a Google Firebase platform that can handle significant users and data. It can be used onsite or remotely, allowing for code compliance to continue despite ongoing pandemic related delays or shutdowns for local economies. The information provided by the software tool allows users to uniformly assess a system for compliance and use that aggregated data to identify training topics or create internal process designed to improving issues and reducing occurrence. This solution has multiple benefits in managing quality at time of use and promoting an increase in future safety and quality through education. Perhaps most importantly, this software increases public safety by ensuring compliance of installed systems and allows for local AHJs to remotely engage specialized and qualified solar specific expertise for oversite of the installation in their jurisdictions. Data analysis provides the quality feedback loop identifying the root cause of failure and drives installation practices to improve through training and education, resulting in systems with higher performance, greater reliability, and reduced operations and maintenance costs. With the successful completion of this project, the industry can expect reduced soft costs and increased performance and safety and will ultimately benefit from longer performing systems that cost less to operate.

14 SOLAR ENERGY↗

Final Technical Report-WestSmart EV: Western Smart Plug-in Electric Vehicle Community Partnership

The WestSmartEV (WSEV) project has accelerated adoption of plug-in electric vehicles (PEV) throughout the PacifiCorp/Rocky Mountain Power’s (RMP) service territory in the intermountain west by developing a large-scale, sustainable PEV charging infrastructure network with coordinated PEV adoption programs. The project objectives have strategically deployed 79 DC fast charging to create two primary electric interstate highway corridors along I-15 and I-80; incentivized installation of Level 2 AC chargers at workplace locations; incentivized the purchase of PEVs; provided all electric solutions for first-mile and last-mile trips, including electrified mobility service; provided centralized data collection, analysis, modeling, and tool development to inform investment and policy decisions; and developed education outreach materials and conducted workshops across the WSEV region.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

Extravehicular Activity (EVA) & Human Surface Mobility (HSM) Program (EHP) Joint EVA & HSM Test Team (JETT) Field Test 3 (JETT3) Report

The purpose of this field test and corresponding report is to evolve the lunar surface EVA concept of operations and hardware via developmental testing. This report compiles and summarizes the goals, infrastructure, test configuration, conclusions, and recommendations from the JETT3 field test. These insights are intended to inform future integrated testing efforts and broader Artemis campaign development activities.

NASA↗

Usability Evaluation of Fleet Management Interface for High Density Vertiplex Environments

To meet the rising demand for an Advanced Air Mobility (i.e. urban and rural unmanned aircraft systems) ecosystem, NASA Aeronautics Research Mission Directorate (ARMD) is hosting a series of simulations and flight tests under the High Density Vertiplex sub-project (HDV). HDV aims to develop an integrated automation architecture to support terminal area flight operations. The HDV simulations and flight tests address safety, integration, and operational challenges, while integrated systems and software demonstrate design readiness, robustness, and interoperability. During the initial HDV simulation in 2021, a prototype traffic management tool developed by NASA called Fleet Management Interface (FMI) was tested. FMI was designed to introduce an advanced level of human-automation interaction to aid both Ground Control Station Operators (GCSOs) and Fleet Managers (FMs) in remotely managing flights under their ownership. In a human-in-the-loop simulation, a usability study was conducted with FMI to identify optimal approaches for displaying information for human operators using subjective measures of usability, workload, situation awareness, risk, and trust, along with qualitative feedback. This study consisted of task analysis in which GCSO and FM subjects used an Urban Air Mobility (UAM) environment to develop and execute a plan for two different traffic scenarios of remotely controlled vehicles. In each scenario, a controlled vehicle completed a takeoff, active flight, and landing sequence while automated traffic flew in the background at a rate of 20 operations per hour. In the first scenario, the controlled vehicle flew a nominal route with takeoff and landing at the same vertiport. In the second scenario, the controlled vehicle started on the nominal route, then diverted to an unplanned location mid-flight. Results showed that self-reported performance, usability, trust, and situation awareness ratings of FMI were moderately to strongly high. There were small differences between scenarios, with Scenario 2 being perceived as more unstable, complex, variable, risky, and potentially harmful than Scenario 1. Furthermore, participants described improvements that could be made to create a better user experience. For example, users would like greater configurability of the interface based on their personal information requirements, and they would like the opportunity to review routes before assigning them. The results from this study will inform future development of the FMI with the end goal of creating a reference automation tool for airspace management procedures in AAM. The FMI could be introduced as a potential way to reduce dependency on traditional air navigation services through increased automation in high density vertiplex environments.

vertiplex↗

Virus-induced gene editing of stomatal regulators in Nicotiana benthamiana enables rapid functional genomics

Virus-induced gene editing (VIGE) holds promise as a rapid and scalable approach for functional genomics in plants. Here, we apply a tobacco rattle virus (TRV)-based single-guide RNA (sgRNA) delivery system to target key regulators of stomatal development in Nicotiana benthamiana using transgenic Cas9-expressing lines. sgRNAs fused to a mobile RNA element and co-delivered with TRV enabled both somatic and heritable genome editing across orthologs of STOMAGEN, EPF2, YODA, and SPEECHLESS. Somatic editing frequencies reached up to 95%, and heritable tetra-allelic mutations were recovered in multiple target genes. Mutants exhibited significant, gene-specific changes in stomatal density, with corresponding effects on leaf temperature indicative of altered evaporative cooling. Additionally, sgRNAs fused to an AmCyan reporter enabled visualization of virus-infected tissues, allowing stomatal phenotyping in edited M0 sectors. This TRV-based platform facilitates functional assessment of genes influencing stomatal patterning and offers a powerful tool for dissecting gene function in a developmentally and physiologically relevant context.

60 APPLIED LIFE SCIENCES↗

Mixed-flow design for microfluidic printing of two-component polymer semiconductor systems

The rational creation of two-component conjugated polymer systems with high levels of phase purity in each component is challenging but crucial for realizing printed soft-matter electronics. Here, we report a mixed-flow microfluidic printing (MFMP) approach for two-component π -polymer systems that significantly elevates phase purity in bulk-heterojunction solar cells and thin-film transistors. MFMP integrates laminar and extensional flows using a specially microstructured shear blade, designed with fluid flow simulation tools to tune the flow patterns and induce shear, stretch, and pushout effects. This optimizes polymer conformation and semiconducting blend order as assessed by atomic force microscopy (AFM), transmission electron microscopy (TEM), grazing incidence wide-angle X-ray scattering (GIWAXS), resonant soft X-ray scattering (R-SoXS), photovoltaic response, and field effect mobility. For printed all-polymer (poly[(5,6-difluoro-2-octyl-2H-benzotriazole-4,7-diyl)-2,5-thiophenediyl[4,8-bis[5-(2-hexyldecyl)-2-thienyl]benzo[1,2-b:4,5-b′]dithiophene-2,6-diyl]-2,5-thiophenediyl]) [J51]:(poly{[N,N′-bis(2-octyldodecyl)naphthalene-1,4,5,8-bis(dicarboximide)-2,6-diyl]-alt-5,5′-(2,2′-bithiophene)}) [N2200]) solar cells, this approach enhances short-circuit currents and fill factors, with power conversion efficiency increasing from 5.20% for conventional blade coating to 7.80% for MFMP. Moreover, the performance of mixed polymer ambipolar [poly(3-hexylthiophene-2,5-diyl) (P3HT):N2200] and semiconducting:insulating polymer unipolar (N2200:polystyrene) transistors is similarly enhanced, underscoring versatility for two-component π -polymer systems. Mixed-flow designs offer modalities for achieving high-performance organic optoelectronics via innovative printing methodologies.

42 ENGINEERING↗

Final Technical Report for DE-SC0022206

This project developed foundational genetic, genomic, and epigenetic tools for anaerobic fungi (Neocallimastigomycota), a group of microorganisms with exceptional natural abilities to deconstruct lignocellulosic biomass. Efficient biomass deconstruction remains a major barrier to economical production of renewable fuels, chemicals, and materials from agricultural and forestry residues. The project sought to enable mechanistic studies and future engineering of anaerobic fungi by improving genomic resources, establishing methods for gene expression, and investigating epigenetic regulation of biomass-degrading pathways. Major accomplishments included generation of the first chromosome-scale genome assemblies for multiple anaerobic fungal species, providing publicly available genomic resources that support both engineering and fundamental biological research. The project established the first reproducible system for heterologous gene expression in anaerobic fungi and identified genomic features and mobile genetic elements that may support future development of stable transformation technologies. In parallel, the project demonstrated direct conversion of untreated lignocellulosic biomass into fuels and specialty chemicals through a fungal-yeast bioprocess and identified anaerobic fungal enzymes with utility for metabolic engineering. The research also revealed that epigenetic regulation plays an important role in controlling fungal gene expression and enzyme production, identifying potential strategies for enhancing biomass degradation. Collectively, this work established anaerobic fungi as a tractable emerging platform for bioenergy and biomanufacturing research, generated valuable public resources, trained the next generation of researchers, and advanced DOE-BER goals related to predictive biology, sustainable bioprocessing, and the circular bioeconomy.

Solomon, Kevin [University of Delaware] (ORCID:000↗