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Improved Scanners for Microscopic Hyperspectral Imaging

Improved scanners to be incorporated into hyperspectral microscope-based imaging systems have been invented. Heretofore, in microscopic imaging, including spectral imaging, it has been customary to either move the specimen relative to the optical assembly that includes the microscope or else move the entire assembly relative to the specimen. It becomes extremely difficult to control such scanning when submicron translation increments are required, because the high magnification of the microscope enlarges all movements in the specimen image on the focal plane. To overcome this difficulty, in a system based on this invention, no attempt would be made to move either the specimen or the optical assembly. Instead, an objective lens would be moved within the assembly so as to cause translation of the image at the focal plane: the effect would be equivalent to scanning in the focal plane. The upper part of the figure depicts a generic proposed microscope-based hyperspectral imaging system incorporating the invention. The optical assembly of this system would include an objective lens (normally, a microscope objective lens) and a charge-coupled-device (CCD) camera. The objective lens would be mounted on a servomotor-driven translation stage, which would be capable of moving the lens in precisely controlled increments, relative to the camera, parallel to the focal-plane scan axis. The output of the CCD camera would be digitized and fed to a frame grabber in a computer. The computer would store the frame-grabber output for subsequent viewing and/or processing of images. The computer would contain a position-control interface board, through which it would control the servomotor. There are several versions of the invention. An essential feature common to all versions is that the stationary optical subassembly containing the camera would also contain a spatial window, at the focal plane of the objective lens, that would pass only a selected portion of the image. In one version, the window would be a slit, the CCD would contain a one-dimensional array of pixels, and the objective lens would be moved along an axis perpendicular to the slit to spatially scan the image of the specimen in pushbroom fashion. The image built up by scanning in this case would be an ordinary (non-spectral) image. In another version, the optics of which are depicted in the lower part of the figure, the spatial window would be a slit, the CCD would contain a two-dimensional array of pixels, the slit image would be refocused onto the CCD by a relay-lens pair consisting of a collimating and a focusing lens, and a prism-gratingprism optical spectrometer would be placed between the collimating and focusing lenses. Consequently, the image on the CCD would be spatially resolved along the slit axis and spectrally resolved along the axis perpendicular to the slit. As in the first-mentioned version, the objective lens would be moved along an axis perpendicular to the slit to spatially scan the image of the specimen in pushbroom fashion.

Mao, Chengye↗

Hyperspectral Imaging on the International Space Station: An Innovative Approach to Commercial Development of Space

NASA s Space Partnership Division (SPD) was established to promote the commercial development of space by providing access to space ai opportunity to perform commercial research in the microgravity environment. NASA, through SPD, has established Research Partnership Centers (RPC s) that bring the government, universities at private industry together to perform research in space for commercial applica!.!lons. The SPD Office has fostered a re!ationship between an RPC and an aerospace company to perform hyperspectral imaging on the Window Observational Research Facility (WORF) on board the International Space Station (ISS). As a result of this relationship and M the capabilities of the WORF, the ISS will serve the private sector with platform to conduct hyperspectral imaging for commercial research.

Source record↗

Onboard Hyperspectral Image Classification via Transfer Learning for Communication-Limited Spacecraft

Employing deep-learning and artificial-intelligence (AI) techniques onboard spacecraft can dramatically improve priority data selection to ensure more effective use of the available downlink. However, deployment of effective deep-learning models requires significant training on the ground, which may not be feasible, due to limited data available in an unexplored environment. Therefore, this research explores building robust classification models for onboard data processing where training data is highly limited using transfer-learning techniques. In this paper, we focus on the use case of hyperspectral imaging for remote sensing, a domain where the high dimensionality of the data from the sensor can rapidly saturate the downlink bandwidth. With this bottleneck, there is an impending need to autonomously and robustly classify data onboard to optimize downlink of high-impact measurements, thus maximizing the scientific utility per bit transmitted to the ground. This paper examines the use of deep neural networks onboard for hyperspectral image classification in a communication-limited scenario to analyze how the models perform with limited training data. The use of transfer learning can ameliorate the issue of poor generalization by transferring features learned from training on a large source dataset for one classification task to the target classification task with limited training data. For two deep-learning models from literature, we compare the accuracy of the models trained using transfer learning to models trained from scratch using a random weight initialization with varying amounts of training data. We demonstrate the feasibility and performance of running inference of the deep-learning models on representative flight-like hardware.

Advanced Avionics, Machine Learning, Data Processi↗

Target Detection Using an AOTF Hyperspectral Imager

This paper reports results of a recent field experiment using a prototype system to evaluate the acousto-optic tunable filter polarimetric hyperspectral imaging technology for target detection applications.

acousto-optic tunable filter polarimetric hyperspe↗

ICER-3D: A Progressive Wavelet-Based Compressor for Hyperspectral Images

ICER-3D is a progressive, wavelet-based compressor for hyperspectral images. ICER-3D is derived from the ICER image compressor. ICER-3D can provide lossless and lossy compression, and incorporates an error-containment scheme to limit the effects of data loss during transmission. The three-dimensional wavelet decomposition structure used by ICER-3D exploits correlations in all three dimensions of hyperspectral data sets, while facilitating elimination of spectral ringing artifacts. Correlation is further exploited by a context modeler that effectively exploits spectral dependencies in the wavelet-transformed hyperspectral data. Performance results illustrating the benefits of these features are presented.

Kiely, A.↗

Atmospheric Correction for Hyperspectral Ocean Color Retrieval with Application to the Hyperspectral Imager for the Coastal Ocean (HICO)

The classical multi-spectral Atmospheric Correction (AC) algorithm is inadequate for the new generation of spaceborne hyperspectral sensors such as NASA's first hyperspectral Ocean Color Instrument (OCI) onboard the anticipated Plankton, Aerosol, Cloud, ocean Ecosystem (PACE) satellite mission. The AC process must estimate and remove the atmospheric path radiance contribution due to the Rayleigh scattering by air molecules and scattering by aerosols from the measured top-of-atmosphere (TOA) radiance, compensate for the absorption by atmospheric gases, and correct for reflection and refraction of the air-sea interface. In this work, we present and evaluate an improved AC for hyperspectral sensors developed within NASA's Sea-viewing Wide Field-of-view Sensor (SeaWiFS) Data Analysis System software package (SeaDAS). The improvement is based on combining the classical AC approach of multi-spectral capabilities to correct for the atmospheric path radiance, extended to hyperspectral, with a gas correction algorithm to compensate for absorbing gases in the atmosphere, including water vapor. The SeaDAS-hyperspectral version is capable of operationally processing the AC of any hyperspectral airborne or spaceborne sensor. The new algorithm development was evaluated and assessed using the Hyperspectral Imager for Coastal Ocean (HICO) scenes collected at the Marine Optical BuoY (MOBY) site, and other SeaWiFS Bio-optical Archive and Storage System (SeaBASS) and AERosol Robotic NETwork - Ocean Color (AERONET-OC) coastal sites. A hyperspectral vicarious calibration was applied to HICO, showing the validity and consistency of HICO's ocean color products. The hyperspectral AC capability is currently available in SeaDAS to the scientific community at https://oceancolor.gsfc.nasa.gov/.

Ocean Color↗

A Blind Convolutional Deep Autoencoder for Spectral Unmixing of Hyperspectral Images Over Waterbodies

Harmful algal blooms have dangerous repercussions for biodiversity, the ecosystem, and public health. Automatic identification based on remote sensing hyperspectral image analysis provides a valuable mechanism for extracting the spectral signatures of harmful algal blooms and their respective percentage in a region of interest. This paper proposes a new model called a non-symmetrical autoencoder for spectral unmixing to perform endmember extraction and fractional abundance estimation. The model is assessed in benchmark datasets, such as Jasper Ridge and Samson. Additionally, a case study of the HSI2 image acquired by NASA over Lake Erie in 2017 is conducted for extracting optical water types. The results using the proposed model for the benchmark datasets improve unmixing performance, as indicated by the spectral angle distance compared to five baseline algorithms. Improved results were obtained for various metrics. In the Samson dataset, the proposed model outperformed other methods for water (0.060) and soil (0.025) endmember extraction. Moreover, the proposed method exhibited superior performance in terms of mean spectral angle distance compared to the other five baseline algorithms. The non-symmetrical autoencoder for the spectral unmixing approach achieved better results for abundance map estimation, with a root mean square error of 0.091 for water and 0.187 for soil, compared to the ground truth. For the Jasper Ridge dataset, the non-symmetrical autoencoder for the spectral unmixing model excelled in the tree (0.039) and road (0.068) endmember extraction and also demonstrated improved results for water abundance maps (0.1121). The proposed model can identify the presence of chlorophyll-a in waterbodies. Chlorophyll-a is an essential indicator of the presence of the different concentrations of macrophytes and cyanobacteria. The non-symmetrical autoencoder for spectral unmixing achieves a value of 0.307 for the spectral angle distance metric compared to a reference ground truth spectral signature of chlorophyll-a. The source code for the proposed model, as implemented in this manuscript, can be found at https://github.com/EstefaniaAlfaro/autoencoder_owt_spectral.git.

hyperspectral imaging↗

A Polarimetric Hyperspectral Imaging Sensor

This paper reports recent field test results of a polarimetric hyperspectral imaging prototype sensor using a noncollinear acousto-optic tunable filter as a wavelength sorter and a polarizing beam splitter. The objective of this work is to evaluate the AOTF-PHI technology for a variety of remote sensing applications.

spectral bandpass filter acousto-optic tunable fil↗

Hyperspectral Imager for the Coastal Ocean (HICO): Overview, Operational Updates, and Coastal Ocean Applications

The Hyperspectral Imager for the Coastal Ocean (HICO) was built to measure in‐water properties of complex coastal regions. HICO enables synoptic coverage; 100‐meter spatial resolution for sampling the variability and spatial irregularity of coastal waters; and high spectral resolution to untangle the signals from chlorophyll, colored dissolved organic matter, suspended sediments and varying bottom types. HICO was built by the Naval Research Laboratory, installed on the International Space Station (ISS) in September 2009, and operated for ONR for the first three years. In 2013, NASA assumed sponsorship of operations in order to leverage HICO's ability to address their Earth monitoring mission. This has opened up access of HICO data to the broad research community. Over 8000 images are now available on NASA's Ocean Color Website (http://oceancolor.gsfc.nasa.gov/cgi/browse.pl?sen=hi). Oregon State University's HICO website (http://hico.coas.oregonstate.edu) remains the portal for researchers to request new collections and access their requested data. We will present updates on HICO's calibration and improvements in geolocation and show examples of the use of HICO data to address issues in the coastal ocean and Great Lakes.

Davis, Curtiss O.↗

Metric Learning for Hyperspectral Image Segmentation

We present a metric learning approach to improve the performance of unsupervised hyperspectral image segmentation. Unsupervised spatial segmentation can assist both user visualization and automatic recognition of surface features. Analysts can use spatially-continuous segments to decrease noise levels and/or localize feature boundaries. However, existing segmentation methods use tasks-agnostic measures of similarity. Here we learn task-specific similarity measures from training data, improving segment fidelity to classes of interest. Multiclass Linear Discriminate Analysis produces a linear transform that optimally separates a labeled set of training classes. The defines a distance metric that generalized to a new scenes, enabling graph-based segmentation that emphasizes key spectral features. We describe tests based on data from the Compact Reconnaissance Imaging Spectrometer (CRISM) in which learned metrics improve segment homogeneity with respect to mineralogical classes.

Compact Reconnaissance Imaging Spectrometer (CRISM↗

The Emerging Issue-3 Revision of the Ccsds-123.0-B Low-Complexity Lossless and Near-Lossless Multispectral and Hyperspectral Image Compression Standard

The CCSDS-123.0-B Low-Complexity Lossless and Near-Lossless Multispectral & Hyperspectral Image Compression standard provides state-of-the-art compression for imaging spectrometer data. Issue 1 of this standard, published in 2012, provides only lossless compression capability. In 2019, Issue 2 was published, adding new features and in particular augmenting the compressor to provide also near-lossless compression, i.e., compression with a user-specified error limit in each spectral band. This presentation will describe the Issue 3 revision currently under development by the CCSDS Data Compression working group. This revision will provide region-of-interest compression capability. That is, a user-provided spatial classification map can be used to specify different data fidelity parameters in different image regions. This would, for example, allow an onboard classification algorithm to identify the most important regions of an image, or regions obscured by clouds, and adjust compression fidelity accordingly in each region to maximize value of imaging spectrometer data returned over constrained space communication links. The revision also aims to add an option that is unrelated to region-of-interest compression but can improve compression effectiveness in some cases.

CCSDS Hyperspectral↗

Hyperspectral Image Classification using a Self-Organizing Map

The use of hyperspectral data to determine the abundance of constituents in a certain portion of the Earth's surface relies on the capability of imaging spectrometers to provide a large amount of information at each pixel of a certain scene. Today, hyperspectral imaging sensors are capable of generating unprecedented volumes of radiometric data. The Airborne Visible/Infrared Imaging Spectrometer (AVIRIS), for example, routinely produces image cubes with 224 spectral bands. This undoubtedly opens a wide range of new possibilities, but the analysis of such a massive amount of information is not an easy task. In fact, most of the existing algorithms devoted to analyzing multispectral images are not applicable in the hyperspectral domain, because of the size and high dimensionality of the images. The application of neural networks to perform unsupervised classification of hyperspectral data has been tested by several authors and also by us in some previous work. We have also focused on analyzing the intrinsic capability of neural networks to parallelize the whole hyperspectral unmixing process. The results shown in this work indicate that neural network models are able to find clusters of closely related hyperspectral signatures, and thus can be used as a powerful tool to achieve the desired classification. The present work discusses the possibility of using a Self Organizing neural network to perform unsupervised classification of hyperspectral images. In sections 3 and 4, the topology of the proposed neural network and the training algorithm are respectively described. Section 5 provides the results we have obtained after applying the proposed methodology to real hyperspectral data, described in section 2. Different parameters in the learning stage have been modified in order to obtain a detailed description of their influence on the final results. Finally, in section 6 we provide the conclusions at which we have arrived.

Martinez, P.↗

Hyperspectral Imaging and Spectroscopy of Fluorescently Coupled Acyl-CoA: Cholesterol Acyltransferase in Insect Cells

A high-performance hyperspectral imaging module with high throughput of light suitable for low-intensity fluorescence microscopic imaging and subsequent analysis, including single-pixel-defined emission spectroscopy, was tested on Sf21 insect cells expressing green fluorescence associated with recombinant green fluorescent protein linked or not with the membrane protein acyl-CoA:cholesterol acyltransferase. The imager utilized the phenomenon of optical activity as a new technique providing information over a spectral range of 220-1400 nm, and was inserted between the microscope and an 8-bit CCD video-rate camera. The resulting fluorescence image did not introduce observable image aberrations. The images provided parallel acquisition of well resolved concurrent spatial and spectral information such that fluorescence associated with green fluorescent protein alone was demonstrated to be diffuse within the Sf21 insect cell, and that green fluorescence associated with the membrane protein was shown to be specifically concentrated within regions of the cell cytoplasm. Emission spectra analyzed from different regions of the fluorescence image showed blue shift specific for the regions of concentration associated with the membrane protein.

Malak, H.↗

Real-Time Adaptive Lossless Hyperspectral Image Compression using CCSDS on Parallel GPGPU and Multicore Processor Systems

The proposed CCSDS (Consultative Committee for Space Data Systems) Lossless Hyperspectral Image Compression Algorithm was designed to facilitate a fast hardware implementation. This paper analyses that algorithm with regard to available parallelism and describes fast parallel implementations in software for GPGPU and Multicore CPU architectures. We show that careful software implementation, using hardware acceleration in the form of GPGPUs or even just multicore processors, can exceed the performance of existing hardware and software implementations by up to 11x and break the real-time barrier for the first time for a typical test application.

realtime↗

The Hyperspectral Imager Aboard the SSTI's Lewis Spacecraft: A Comparison with AVIRIS

This paper compares the performance and operational parameters of the Hyperspectral Imager (HSI), scheduled for launch aboard the Lewis spacecraft in July 1996, with those of the Airborne Visible/Infrared Imaging Spectrometer (AVIRIS). The HSI is a pushbroom, imaging spectrometer with 30 meter spatial resolution, generating 384 spectral channels over the range 400 to 2500 nm at 5.0 to 6.4 nm resolution for each of its 256 crosstrack pixels.

Sokolowski, James K.↗