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At least 55 records · Page 3

Magnetometer networks during the International Magnetosphere Study

The paper describes the geographical layout of the planned North American IMS magnetometer network and outlines some plans regarding instrumentation and data transmission services. The network consists of three meridional high-latitude chains, an east-west chain along the auroral zone, and a network of stations at mid-latitudes. In addition, the Air Force Cambridge Research Laboratories will have their own network with an east-west chain along geomagnetic latitude 54 N and some other mid-latitude stations. The preliminary magnetometer specifications require that the three-component flux gate magnetometer be accurate to within 0.25 gamma and that each axis be sampled every 10 sec. A block diagram is presented showing the proposed magnetometer station, of which the most complex piece of equipment will be the interface controller. The SMS-GOES satellite telemetry relay capability will be used for transmission of data from a selected network of magnetic stations to the Space Environment Laboratory in Boulder.

Lanzerotti, L. J.↗

Insights on carbon dioxide adsorption in a flexible 1-D coordination polymer from in situ X-ray scattering and density functional theory

A combination of in situ small-angle X-ray scattering (SAXS) microstructure characterization over scales ranging from 1 nm to 10 µm and powder X-ray diffraction (XRD) structure characterization under various gas/pressure/temperature conditions with density functional theory (DFT) calculations provides new insights for the CO2 sorption behavior of a one-dimensional porous coordination polymer: catena-bis­(di­benzoyl­methanato)(4,4′-bi­pyridyl)­nickel(II), denoted NiDBM-Bpy. The NiDBM-Bpy chains are held together by van der Waals forces, but the structure of guest-free NiDBM-Bpy is unsolved due to a lack of suitable crystals and high-quality powder XRD patterns. Nevertheless, SAXS and powder XRD can follow microstructural and structural changes as a function of gas pressure, composition and temperature. Both mixed-gas flow and static supercritical CO2 regimes are explored experimentally. DFT calculations are used to model the structural variation associated with XRD changes and hysteresis in the sorption isotherms. XRD and DFT calculations suggest that an orthorhombic Fddd structure with two CO2 per Ni emerges following a transition from the structure with lower molar volume and symmetry that exists without CO2 present.

Allen, Andrew J.↗

The LEMUR 3 Limbed Climbing Robot

This paper introduces a new four-limbed robot, LEMUR 3, that has demonstrated climbing on cliff faces and smooth glass. Each limb on the robot consists of seven identical actuators in a serial chain. Each limb terminates in a single axis force sensor that allows various end effectors to be mounted and connected to the robot’s power and communication system. Microspine grippers were used for climbing the rocky surface and gecko adhesive grippers were used for the glass solar panels. All other hardware and much of the software was common for the two demonstrations. The robot’s mechanical, electrical, and software systems, various gripping devices, and field demonstrations are described. Limbed mobility is of interest to JPL and NASA because of its potential to access extreme terrain, including that on Mars and in microgravity environments.

Kennedy, Brett↗

Enhancements of Nucleate Boiling Under Microgravity Conditions

This paper presents two means for enhancing nucleate boiling and critical heat flux under microgravity conditions: using micro-configured metal-graphite composites as the boiling surface and dilute aqueous solutions of long-chain alcohols as the working fluid. In the former, thermocapillary force induced by temperature difference between the graphite-fiber tips and the metal matrix plays an important role in bubble detachment. Thus boiling-heat transfer performance does not deteriorate in a reduced-gravity environment. In the latter cases, the surface tension-temperature gradient of the long-chain alcohol solutions turns positive as the temperature exceeds a certain value. Consequently, the Marangoni effect does not impede, but rather aids in bubble departure from the heating surface. This feature is most favorable in microgravity. As a result, the bubble size of departure is substantially reduced at higher frequencies. Based on the existing experimental data, and a two-tier theoretical model, correlation formulas are derived for nucleate boiling on the copper-graphite and aluminum-graphite composite surfaces, in both the isolated and coalesced bubble regimes. In addition, performance equations for nucleate boiling and critical heat flux in dilute aqueous solutions of long-chain alcohols are obtained.

Zhang, Nengli↗

Physics and Modeling of Quasi-Steady and Forced Shock Trains

The dual-mode scramjet is a propulsive concept for contemporary hypersonic vehicles promising flight cruise speeds upwards of Mach 5. Despite decades of development and a recent resurgence in interest, such engines are not routinely deployed for commercial or military purposes. Instead, they have been confined to the realm of ground-test research and limited one-off flight test missions. One of the key stumbling blocks on the critical path to deployment of a dual-mode scramjet is the optimal design and stable operation of the isolator, a typically constant area duct that separates the scramjet inlet from the combustor. During ramjet mode at Mach numbers from 3 to 5 and during the transition to scramjet mode at higher Mach numbers, the isolator contains the pseudo-shock. Necessary to meet the back-pressure in the combustor, the pseudo-shock is typically divided into a shock dominated shock train region followed by a turbulence driven mixing region. Fluctuations in combustor pressure due to vehicle maneuvering, throttling, or combustion instabilities drive the shock train to shift streamwise within the isolator. In response to sharp pressure fluctuations, the shock train is unable to stabilize within the isolator, and the scramjet unstarts by ejecting the shock train out the inlet. This work addresses three key gaps in understanding of the isolator pseudo-shock. The phenomenon is studied in three experimental facilities to investigate the quasi-steady and unsteady fluid dynamics of the shock train in fueled and un-fueled test conditions. The strong link between boundary layer morphology and shock train structure is studied in detail and used as a tool to evaluate the state-of-the-art definitions and models of the pseudo-shock. Identifying the limitations of these models, a new process driven flux-conserved pseudo-shock model is proposed. This provides a computationally efficient model grounded in the fluid dynamic mechanisms of the pseudo-shock with better predictive capability than the current state-of-the-art across all facilities tested. The unsteady behavior of the shock train is further investigated to identify and understand two distinct forced dynamic modes: mechanically induced, low-rate forcing and high-rate combustion driven unsteadiness that sheds upstream propagating shocks. The proposed process driven model is found to be effective in the the limiting case of high-rate combustion driven unsteadiness by evaluating the model in a shock stationary reference frame. Further analysis identifies the approximately rigid chain motion of the shock train structure to mechanically induced forcing. The time delay between the onset of forced dynamics is found to saturate to a constant value and maximum shock train speed along the isolator with respect to the perturbation rate of change. The result of this work provides dual-mode scramjet designers with improved tools and physical understanding to develop more efficient, robust, and capable propulsion systems for hypersonic vehicles.

JAXA↗

Effect of spaceflight on skeletal muscle: Mechanical properties and myosin isoform content of a slow muscle

This study examined changes in contractile, biochemical, and histochemical properties of slow antigravity skeletal muscle after a 6-day spaceflight mission. Twelve male Sprague-Dawley rats were randomly divided into two groups: flight and ground-based control. Approximately 3 h after the landing, in situ contractile measurements were made on the soleus muscles of the flight animals. The control animals were studied 24 h later. The contractile measurements included force-velocity relationship, force-frequency relationship, and fatigability. Biochemical measurements focused on the myosin heavy chain (MHC) and myosin light chain profiles. Adenosinetriphosphatase histochemistry was performed to identify cross-sectional area of slow and fast muscle fibers and to determine the percent fiber type distribution. The force-velocity relationships of the flight muscles were altered such that maximal isometric tension P(sub o) was decreased by 24% and maximal shortening velocity was increased by 14% (P less than 0.05). The force-frequency relationship of the flight muscles was shifted to the right of the control muscles. At the end of the 2-min fatigue test, the flight muscles generated only 34% of P(sub o), whereas the control muscles generated 64% of P(sub o). The flight muscles exhibited de novo expression of the type IIx MHC isoform as well as a slight decrease in the slow type I and fast type IIa MHC isoforms. Histochemical analyses of flight muscles demonstrated a small increase in the percentage of fast type II fibers and a greater atrophy of the slow type I fibers. The results demonstrate that contractile properties of slow antigravity skeletal muscle are sensitive to the microgravity environment and that changes begin to occur within the 1st wk. These changes were at least, in part, associated with changes in the amount and type of contractile protein expressed.

Caiozzo, Vincent J.↗

Experiments in cooperative-arm object manipulation with a two-armed free-flying robot

Developing computed-torque controllers for complex manipulator systems using current techniques and tools is difficult because they address the issues pertinent to simulation, as opposed to control. A new formulation of computed-torque (CT) control that leads to an automated computer-torque robot controller program is presented. This automated tool is used for simulations and experimental demonstrations of endpoint and object control from a free-flying robot. A new computed-torque formulation states the multibody control problem in an elegant, homogeneous, and practical form. A recursive dynamics algorithm is presented that numerically evaluates kinematics and dynamics terms for multibody systems given a topological description. Manipulators may be free-flying, and may have closed-chain constraints. With the exception of object squeeze-force control, the algorithm does not deal with actuator redundancy. The algorithm is used to implement an automated 2D computed-torque dynamics and control package that allows joint, endpoint, orientation, momentum, and object squeeze-force control. This package obviates the need for hand-derivation of kinematics and dynamics, and is used for both simulation and experimental control. Endpoint control experiments are performed on a laboratory robot that has two arms to manipulate payloads, and uses an air bearing to achieve very-low drag characteristics. Simulations and experimental data for endpoint and object controllers are presented for the experimental robot - a complex dynamic system. There is a certain rather wide set of conditions under which CT endpoint controllers can neglect robot base accelerations (but not motions) and achieve comparable performance including base accelerations in the model. The regime over which this simplification holds is explored by simulation and experiment.

Koningstein, Ross↗

Investigating the Strength and Variability of El Niño Southern Oscillation Teleconnections to Hydroclimate and Maize Yields in Southern and East Africa

The state of the El Niño Southern Oscillation (ENSO) is critical for seasonal climate forecasts, but recent events diverged substantially from expectations in many regions, including Sub-Saharan Africa where seasonal forecasts are critical tools for addressing food security. Here, we evaluate 39 years (1982–2020) of data on hydroclimate, leaf area index, and maize yields to investigate the strength of ENSO teleconnections in southern and East Africa. Teleconnections to precipitation, soil moisture, and leaf area index are generally stronger during ENSO phases that cause drought conditions (El Niño in southern Africa and La Niña in East Africa), with seasonality that aligns well with the maize growing seasons. Within maize growing areas, however, ENSO teleconnections to hydroclimate and vegetation are generally weaker compared to the broader geographic regions, especially in East Africa. There is also little evidence that the magnitude of the ENSO event affects the hydroclimate or vegetation response in these maize regions. Maize yields in Kenya, Malawi, South Africa, and Zimbabwe all correlate significantly with hydroclimate and leaf area index, with South Africa and Zimbabwe showing the strongest and most consistent yield responses to ENSO events. Our results highlight the chain of causality from El Niño and La Niña forcing of regional anomalies in hydroclimate to vegetation health and maize yields in southern and East Africa. The large spread across individual ENSO events, however, underscores the limitations of this climate mode for seasonal climate prediction in the region, and the importance of finding additional sources of skill for improving climate and yield forecasts.

El Niño Southern Oscillation↗

The effects of space flight on the contractile apparatus of antigravity muscles: implications for aging and deconditioning

Previous studies have shown that the unloading of skeletal muscle, as occurring during exposure to space flight, exerts a profound effect on both the mass (cross sectional area) of skeletal muscle fibers and the relative expression of protein isoforms comprising the contractile system. Available information suggests that slow (type I) fibers, comprising chiefly the antigravity muscles of experimental animals, in addition to atrophying, undergo alterations in the type of myosin heavy chain (MHC) expressed such that faster isoforms become concomitantly expressed in a sub-population of slow fibers when insufficient force-bearing activity is maintained on the muscle. Consequently, these transformations in both mass and myosin heavy chain phenotype could exert a significant impact on the functional properties of skeletal muscle as manifest in the strength, contractile speed, and endurance scope of the muscle. To further explore these issues, a study was performed in which young adult male rats were exposed to zero gravity for six days, following which, the antigravity soleus muscle was examined for a) contractile properties, determined in situ and b) isomyosin expression, as studied using biochemical, molecular biology, and histochemical/immunohistochemical techniques.

Flight Experiment↗

Investigating PVC polymer–plasticizer interactions with atomistic MD simulations and potential of mean force calculations

For this work, atomistic molecular dynamics (MD) simulations coupled with potential of mean force (PMF) calculations were employed to investigate the interactions between PVC polymer chains containing 6–20 repeating units and various plasticizers, with the goal of identifying potential replacements for the toxic plasticizer di(2-ethylhexyl)phthalate (DEHP) used in blood bags. The selected plasticizers belong to various chemical families, such as orthophthalates, citrates, adipates, and the terephthalate DEHT. Both the polymer and the plasticizers lack ionizable groups, and their interactions are primarily governed by van der Waals and electrostatic forces. A model correlating PMF profiles with interaction forces was developed and validated across polyvinyl chloride (PVC) polymers of different lengths and all investigated plasticizers. This model provides insight into how structural variations in plasticizers influence their respective PMF values. The study ranks the investigated plasticizers based on binding affinity, identifying TOTM (tris(2-ethylhexyl) trimellitate, TEHTM), BTHC (butyryl trihexyl citrate, Citroflex B-6), ATHC (acetyl trihexyl citrate, Citroflex A-6, CA-6), and DEHT (bis(2-ethylhexyl)terephthalate, DOTP/DEHTP) as promising alternatives for further investigation. This comprehensive study encompasses PVC polymers of four different lengths and 14 plasticizers, with all data averaged over 30 independent simulations. The approach provides a deeper understanding of molecular interactions, enabling the tailoring of polymer–plasticizer systems for diverse applications, including extractables and leachables, with relevance spanning materials science to biomedical engineering, and also serves as a basis for developing coarse-grained simulation protocols. Overall, this study provides valuable insights for designing safer and more efficient plasticizer substitutes and is well supported by other studies.

Shet, Sai Athmeeya G. [Sri Sathya Sai Institute of↗

All-Atom Modeling and Simulation of Biopolymer Interface: Dual Role of Antifouling Polymer Brushes

Antifouling polymer brushes are well-known for their exceptional resistance to unwanted protein adsorption. While experimental studies have extensively characterized protein–polymer brush interactions, computational investigations remain limited, largely due to the challenges in accurately modeling and integrating these complex, multicomponent systems without resorting to oversimplification. To address this challenge, this study presents one of the most comprehensive and realistic model systems to date, comprising the substrate, grafted polymers, and proteins. Our work interprets the interactions between polymer brush and protein based on realistic modeling without simplification. In particular, this study utilizes molecular modeling and simulation of polycationic and polyzwitterionic brushes─poly(dimethylaminoethyl methacrylate) (PDMAEMA), poly(2-(N-oxide-N,N-dimethylamino)ethyl methacrylate) (PNOMA), and poly(2-(N-3-sulfopropyl-N,N-dimethylammonium)ethyl methacrylate) (PSBMA)─grafted onto α-quartz substrates via polymerization initiator linkers. The brush models were developed to closely replicate experimentally synthesized samples and to provide detailed insights into structural and dynamical changes at the molecular level during protein adsorption. Using steered molecular dynamics simulations, we show that the PSBMA brush, due to its high local density, exhibits the greatest resistance to protein insertion. Cα root-mean-square deviation and interaction pattern analyses further reveal that the PSBMA brush also induces the most significant destabilization of lysozyme, while the PDMAEMA brush enhances protein stability through ion-mediated interactions. The PNOMA brush, while requiring the lowest force for protein adsorption, induces greater protein destabilization than the PDMAEMA brush, primarily due to electrostatic repulsion caused by a short carbon spacer length. Hydration analysis reveals that both the PSBMA brush and the lysozyme interacting with it exhibit the most rapid dehydration, attributed to the brush’s high local chain density, which results in the greatest lysozyme destabilization and the highest adsorption force. These findings highlight the dual role of antifouling polymer brushes: resisting protein adsorption and modulating protein structural dynamics. In conclusion, this study provides valuable insights for the rational design of next-generation antifouling materials and offers a framework for realistic model development in complex multicomponent systems.

Adsorption↗

The Chain-link Actuator: Exploiting the Bending Stiffness of McKibben Artificial Muscles to Achieve Larger Contraction Ratios

McKibben artificial muscles, comprised of an ex-pandable bladder wrapped in a double-helix-braided sheath,have the ability to generate forces without restricting motion tooccur exclusively along the direction of actuation. This makesthem attractive for a variety of applications including soft,wearable, and biomimetic robots. Despite their advantages, thetheoretical maximum contraction ratio of McKibben muscles isonly 36.3%, which restricts the range of motion of the systemsthey actuate. This work introduces a novel ‘chain-link actuator’that exploits the bending stiffness of McKibben muscles toachieve contraction ratios of more than 50%. A static modelthat captures the relationship between pressure, displacement,and force is presented and validated on several real chain-linkactuator systems.

Daniel Bruder↗

Transport coefficient approach for characterizing nonequilibrium dynamics in soft matter

Nonequilibrium states in soft condensed matter require a systematic approach to characterize and model materials, enhancing predictability and applications. Among the tools, X-ray photon correlation spectroscopy (XPCS) provides exceptional temporal and spatial resolution to extract dynamic insight into the properties of the material. However, existing models might overlook intricate details. We introduce an approach for extracting the transport coefficient, denoted as $J(t)$, from the XPCS studies. This coefficient is a fundamental parameter in nonequilibrium statistical mechanics and is crucial for characterizing transport processes within a system. Our method unifies the Green–Kubo formulas associated with various transport coefficients, including gradient flows, particle–particle interactions, friction matrices, and continuous noise. We achieve this by integrating the collective influence of random and systematic forces acting on the particles within the framework of a Markov chain. We initially validated this method using molecular dynamics simulations of a system subjected to changes in temperatures over time. Subsequently, we conducted further verification using experimental systems reported in the literature and known for their complex nonequilibrium characteristics. The results, including the derived $J(t)$ and other relevant physical parameters, align with the previous observations and reveal detailed dynamical information in nonequilibrium states. This approach represents an advancement in XPCS analysis, addressing the growing demand to extract intricate nonequilibrium dynamics. Further, the methods presented are agnostic to the nature of the material system and can be potentially expanded to hard condensed matter systems.

75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND↗

Monocoque structure for the SKITTER three-legged walker

The SKITTER 2 design is a monocoque version of the proposed lunar three-legged walker. By the definition of monocoque, the body and legs are a shell with no internal ribbing or supports added for absorbing stresses. The purpose of the monocoque is to encase the elements used for power transmission, power supply, and control of the motion. The material for the structure is a vinyl ester resin, Derakane 8084. This material is easily formable and locally obtainable. The body consists of a hexagonally shaped cylinder with truncated hexagonal pyramids on the top and botton. The legs are eight inch diameter cylinders. The legs are comprised of a tibia section and a femur section. The SKITTER 2 is powered by six actuators which provide linear forces that are transformed into rotary torques by a series of chains and sprockets. The joints connect the femur to the body and the tibia to the femur. Surrounding the joints are flexible rubber hoses that fully encase the chains and sprockets. The SKITTER 2 is capable of walking upside down, righting itself after being overturned, and has the ability to perform in many environments. Applications for this walker include lunar transport or drilling, undersea exploration, and operation in severe surroundings such as arctic temperatures or high radiation.

Bansek, Robert N.↗

"Electrostructural Phase Changes" In Charged Particulate Clouds: Planetary and Astrophysical Implications

There is empirical evidence that freely-suspended triboelectrostatically charged particulate clouds of dielectric materials undergo rapid conversion from (nominally) monodispersed "aerosols" to a system of well-defined grain aggregates after grain motion or fluid turbulence ceases within the cloud. In United States Microgravity Laboratory Space Shuttle experiments USML-1 and USML-2, it was found that ballistically-energized grain dispersions would rapidly convert into populations of filamentary aggregates after natural fluid (air) damping of grain motion. Unless continuously disrupted mechanically, it was impossible to maintain a non-aggregated state for the grain clouds of sand-size materials. Similarly, ground- based experiments with very fine dust-size material produced the same results: rapid, impulsive "collapse" of the dispersed grains into well-defined filamentary structures. In both ground-based and microgravity experiments, the chains or filaments were created by long-range dipole electrostatic forces and dipole-induced dielectric interactions, not by monopole interactions. Maintenance of the structures was assisted by short-range static boundary adhesion forces and van der Waals interactions. When the aggregate containers in the USML experiments were disturbed after aggregate formation, the quiescently disposed filaments would rearrange themselves into fractal bundles and tighter clusters as a result of enforced encounters with one another. The long-range dipole interactions that bring the grains together into aggregates are a product of randomly-distributed monopole charges on the grain surfaces. In computer simulations, it has been shown that when the force vectors of all the random charges (of both sign) on a grain are resolved mathematically by assuming Coulombic interaction between them, the net result is a dipole moment on individual grains, even though the grains are electrically neutral insofar as there is no predominance, on their surface, of one charge sign over another. The random charges of both sign derive from natural grain-to-grain interactions that produce triboelectrification via charge exchange every time grain surfaces make contact with one another. The conversion from a random distribution of grains (upon which there are randomly distributed charges) into an organization of electrostatically-ordered aggregates, can be regarded (within the framework of granular-material science) as an "electrical or Coulombic phase change" of the particulate cloud. It is not totally dissimilar from the more normal phase-change concept in which, for example, a gas with long free-path-molecules suddenly becomes a solid as a result of structural ordering of the molecules (notably, also the result of electronic forces, albeit at a different scale). In both the gas-to-solid case, and the aerosol-to-aggregate case, the same materials and charges are present before and after the phase change, but their arrangement now has a higher degree of order and a lower-energy configuration. An input of energy into the system is required to reverse the situation. The aggregates in the USML experiments were observed to undergo at least two phase changes as noted above. The point about phase changes, and by implication, the "electrostructural" reorganizations in particulate clouds, is the following: (a) they can occur very rapidly, almost spontaneously, above a critical cloud density, (b) in going from a higher energy state to a lower energy state, they convert to a denser system, (c) energy must be required to reverse the situation, implying that energy is released during the high-to-low energy phase change. In applying this information to natural particulate clouds, some inferences can be made (it is stressed that reference is still to dielectric materials attracted by dipole forces). There are several natural settings to which the USML observations apply, and to which the phase-change implications likewise apply. Dense clouds of triboelectrically-charged, kinetically-energized grains are to be found in volcanic eruptions (particularly on earth), aeolian dust storms (particularly on Mars), meteorite impact ejecta curtains (on all planets), in "immature" debris rings around planets (e.g., that from which our own Moon may have condensed), and in gravitationally collapsing protoplanetary dust/planetesimal debris disks where dielectric granules are being increasingly brought into collisional relationships with one another (increasing both charge exchange and physical proximity). It is noted that in many of these cases, the degree of electrical charging on the grains is likely to be much higher than that in the USML experiments where charging was not enhanced above the "normal", naturally encountered level for the particular materials (quartz, glass, and various silicate minerals). Application of the phase-change concept suggests that volcanic, aeolian, and impact debris clouds may, under certain circumstances, undergo rapid, impulsive, or even catastrophic collapse into a denser state that will lead to rapid precipitation or fall-out of suspended particulates. Although this idea has been suggested previously by the author , the phase change concept possibly permits some new insights into cloud-system behavior. For example, in a protoplanetary debris disk, the work of gravity may suddenly be enhanced by electrostatically driven collapse of the system when materials reach a critical intergranular spacing or grain number density. This might reduce the rate of planet formation by orders of magnitude (considering the ratio of g-forces to electrostatic ones for very small grains in close proximity), and indeed, it might drive the collapse into a system configuration that would perhaps not be created by gravity alone. Additional information is contained in the original.

Marshall, J. R.↗

Flight Software Dictionary Development for the Mars2020 Rover

The Mars2020 project, developed and operated by the Jet Propulsion Laboratory (JPL), successfully landed the Perseverance rover and its flying companion Ingenuity on the surface of Mars on February 18th 2021. Perseverance combines heritage and cutting-edge flight software and hardware to accomplish crucial mission requirements related to Martian surface sampling. The design, development, and operation of NASA’s large strategic science missions require the ability to communicate spacecraft capabilities to hundreds of engineers across multiple disciplines. The interaction between flight and ground software development, Verification and Validation (V&V), Assembly, Test, and Launch Operations (ATLO), and management each demand quick understanding of unique slices of information for each discipline. This information includes the current capabilities of the flight system as well as future capabilities and their status as they are developed and tested. Despite the fundamental and critical nature of this information, the flight software dictionaries used to track it are a stumbling block for many projects. These dictionaries provide the cornerstone for the interpretation of data sent from the spacecraft, allowing for quick comprehension by engineers on the ground. During both spacecraft development and operations, flight software dictionary management includes significant challenges due to the large number of interfacing systems and the subtle yet distinct needs of each.The engineering of flight software dictionaries for Mars2020 had numerous challenges, most-notably: parallel dictionary development to support simultaneous separate flight software build campaigns for each mission phase (cruise and surface), managing requests for operations-enabling information without perturbing the heritage interface with the rover, and the introduction of new tools by the dictionary stakeholders that forced the dictionary team to innovate and redesign the heritage tool chain. These challenges generated guiding principles for the dictionary development effort: emphasize coding best practices and unit testing in the dictionary code development tool chain, use institutionally provided COTS (commercial-off-the-shelf) tools whenever possible, and maintain the heritage flight-ground interface all while advancing operations-enabling information via a loosely coupled interface.Throughout development and operations, the Mars2020 dictionary toolchain included IBM DOORS Next Generation, GitHub, Microsoft Excel, Docker, Jenkins, and a significant custom-built Python codebase. Significant interfaces included JPL’s command and control software, heritage flight software team tools and processes, and the many cloud-based ground tools developed for the mission.This paper will discuss the requirements for the Mars2020 dictionary development, the development team’s response to those requirements, lessons learned throughout the process, steps taken towards automated deliveries and continuous integration of stakeholder inputs, potential toolchain improvements for Mars2020, and key takeaways that could be applied to future missions.

Pyrzak, Guy↗

BioWires: Conductive DNA Nanowires in a Computationally-Optimized, Synthetic Biological Platform for Nanoelectronic Fabrication

DNA is an ideal template for a biological nanowire-it has a linear structure several atoms thick; it possesses addressable nucleobase geometry that can be precisely defined; and it is massively scalable into branched networks. Until now, the drawback of DNA as a conducting nanowire been, simply put, its low conductance. To address this deficiency, we extensively characterize a chemical variant of canonical DNA that exploits the affinity of natural cytosine bases for silver ions. We successfully construct chains of single silver ions inside double-stranded DNA, confirm the basic dC-Ag+-dC bond geometry and kinetics, and show length-tunability dependent on mismatch distribution, ion availability and enzyme activity. An analysis of the absorbance spectra of natural DNA and silver-binding, poly-cytosine DNA demonstrates the heightened thermostability of the ion chain and its resistance to aqueous stresses such as precipitation, dialysis and forced reduction. These chemically critical traits lend themselves to an increase in electrical conductivity of over an order of magnitude for 11-base silver-paired duplexes over natural strands when assayed by STM break junction. We further construct and implement a genetic pathway in the E. coli bacterium for the biosynthesis of highly ionizable DNA sequences. Toward future circuits, we construct a model of transcription network architectures to determine the most efficient and robust connectivity for cell-based fabrication, and we perform sequence optimization with a genetic algorithm to identify oligonucleotides robust to changes in the base-pairing energy landscape. We propose that this system will serve as a synthetic biological fabrication platform for more complex DNA nanotechnology and nanoelectronics with applications to deep space and low resource environments.

DNA↗