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At least 55 records · Page 3

Engineering temporal accumulation of a low recalcitrance polysaccharide leads to increased C6 sugar content in plant cell walls

Reduced cell wall recalcitrance and increased C6 monosaccharide content are desirable traits for future biofuel crops, as long as these biomass modifications do not significantly alter normal growth and development. Mixed-linkage glucan (MLG), a cell wall polysaccharide only present in grasses and related species among flowering plants, is comprised of glucose monomers linked by both β-1,3 and β-1,4 bonds. Previous data have shown that constitutive production of MLG in barley (Hordeum vulgare) severely compromises growth and development. Here, we used spatio-temporal strategies to engineer Arabidopsis thaliana plants to accumulate significant amounts of MLG in the cell wall by expressing the rice CslF6 MLG synthase using secondary cell wall and senescence-associated promoters. Results using secondary wall promoters were suboptimal. When the rice MLG synthase was expressed under the control of a senescence-associated promoter, we obtained up to four times more glucose in the matrix cell wall fraction and up to a 42% increase in saccharification compared to control lines. Importantly, these plants grew and developed normally. The induction of MLG deposition at senescence correlated with an increase of gluconic acid in cell wall extracts of transgenic plants in contrast to the other approaches presented in this study. MLG produced in Arabidopsis has an altered structure compared to the grass glucan, which likely affects its solubility, while its molecular size is unaffected. The induction of cell wall polysaccharide biosynthesis in senescing tissues offers a novel engineering alternative to enhance cell wall properties of lignocellulosic biofuel crops.

59 BASIC BIOLOGICAL SCIENCES↗

Effect of restricted mobility on RNA content and nucleotide composition and on protein content in motoneurons of spinal cord anterior horns

An investigation into the effect of hypokinesia on the ribonucleic acid (RNA) content, the nucleotide composition, and dynamics of protein content in the motoneuron of the rat spinal cord anterior horns is described. Methodology and findings are presented. The study results showed that the nucleotide composition of the total cellular RNA at all the studied periods of hypokinesia remained unchanged and is characteristic for the cytoplasmic, high polymer ribosomal RNA. This means that with a change in the functional state of the neuron the newly formed RNA of the nerve cell has the same composition of bases as the original RNA that belongs to the ribosomal type.

Gorbunova, A. V.↗

Relative water content of Spruce needles determined by the leaf water content index

Leaf relative water content (RWC) is defined as the volume of water in a leaf divided by the volume at full turgor. Using reflectance factors of wavelengths 0.83 micron and 1.6 microns, a Leaf Water Content Index (LWCI) was derived from the Lambert-Beer Law such that LWCI should equal RWC; LWCI was equal to RWC for Picea pungens, Picea rubens, Liquidambar styraciflua, and Quercus agrifolia. Algebraic manipulation shows that R(1.6)/R(0.83) termed the Moisture Stress Index (MSI), is near-linearly correlated to RWC and to the Equivalent Water Thickness (EWT). Five species tested so far had the same relationship between MSI and EWT, but EWT is not a measure of plant water status.

Hunt, E. Raymond, Jr.↗

Determining moisture content of Traditional Chinese Medicines using a near-infrared LED-based moisture content sensor with spectrum analysis

In Traditional Chinese Medicine (TCM), moisture content (MC) inspection using voltage level or a lock-in amplifier, with a features-based near-infrared light-emitting diode sensor is possible. Abundant absorption response in a wide frequency range and information in both the time-domain and frequency-domain can be obtained, with the square excitation method. However, planeness, heterogeneous particle sizes, and measurement noise negatively affect the time-domain feature during MC inspection. In this work, spectrum analysis (SA) for feature extraction is investigated, to precisely measure the MC of Dutong pills. The principal components for low and high frequencies, are aimed at counteracting the dimensional methods, which are used for supplying the lower dimensional features. The SA experimental results demonstrate that the low frequency components, ranging from the base to the 3rd harmonic components, have a high linear correlation with the MC. The calibration and validation experimental results reveal that the MC can be a monotonic function about the SA amplitudes or first principal component (PC1), with the proposed sensor. This method has the potential to be used for gauging automatic, in situ MCs, particularly for quality control and Process Analytical Technology in TCM. Future research opportunities include improving accuracy and application of the method under field conditions.

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗

First determination of the $CP$ content of D → π + π – π + π – and updated determination of the $CP$ contents of D → π + π – π 0 and D → K + K – π 0

Quantum-correlated ψ(3770)→DD¯ decays collected by the CLEO-c experiment are used to perform a first measurement of $F$ $^{4π}_{+}$, the fractional CP-even content of the self-conjugate decay D → π + π – π + π – , obtaining a value of 0.737 ± 0.028. An important input to the measurement comes from the use of D → $K$ $^{0}_{S}$π + π – and D → $K$ $^{0}_{L}$π + π – decays to tag the signal mode. This same technique is applied to the channels D → π + π – π 0 and D → K + K – π 0 , yielding $F$ $^{πππ0}_{+}$ =1.014 ± 0.045 ± 0.022 and $F$ $^{KKπ0}_{+}$ = 0.734 ± 0.106 ± 0.054, where the first uncertainty is statistical and the second systematic. These measurements are consistent with those of an earlier analysis, based on CP-eigenstate tags, and can be combined to give values of $F$ $^{πππ0}_{+}$ = 0.973 ± 0.017 and $F$ $^{KKπ0}_{+}$ = 0.732 ± 0.055. The results will enable the three modes to be included in a model-independent manner in measurements of the unitarity triangle angle γ using B ∓ → DK ∓ decays, and in time-dependent studies of CP violation and mixing in the D 0 D¯ 0 system.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

Possible signatures of the inflationary particle content: spin-2 fields

We study the imprints of a massive spin-2 field on inflationary observables, and in particular on the breaking of consistency relations. In this setup, the minimal inflationary field content interacts with the massive spin-2 field through dRGT interactions, thus guaranteeing the absence of Boulware-Deser ghostly degrees of freedom. The unitarity requirement on spinning particles, known as Higuchi bound, plays a crucial role for the size of the observable signal.

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗

The Variable Saturation Hydraulic Conductivity Method for Improving Soil Water Content Simulation in EPIC and APEX Models

Core Ideas We propose VSHC for calculating soil water percolation in continuous models. VSHC relates permeability to daily hydraulic properties to estimate hydraulic conductivity. VSHC performed well at field scale and improved estimates of peak streamflows. Soil water percolation is a key process in the life cycle of water in fields, watersheds, and river basins. The Environmental Policy Integrated Climate (EPIC) and the Agricultural Policy/Environmental eXtender (APEX) are continuous models developed for evaluating the environmental effects of agricultural management. Traditionally, these models have simulated soil water percolation processes using a tipping‐bucket approach, with the rate of flow limited by the saturated hydraulic conductivity. This simple approach often leads to inaccuracy in simulating elevated soil water conditions where soil water content (SWC) levels may remain above field capacity under prolonged wet weather periods or limited drainage. To overcome this deficiency, a new submodel, the variable saturation hydraulic conductivity (VSHC) method, was developed for simulating soil water percolation processes using a nonlinear equation to estimate the effective hydraulic conductivity as a function of the SWC and soil properties. The VSHC method was evaluated at three sites in the United States and two sites in Europe. In addition, a numerical solution of the Richards equation was used as a benchmark for SWC comparison. Results show that the VSHC method substantially improves the accuracy of the SWC simulation in long‐term simulations, particularly during wet periods. At the watershed scale, results on the Riesel Y2 watershed indicate that the VSHC method enhances model performance in the high‐flow regime of channel peak flows because of the improved estimation of SWC, which implies that the improved SWC simulation at the field scale is beneficial to hydrologic modeling at the watershed scale.

Doro, Luca↗

Push-pull tracer tests: Their information content and use for characterizing non-Fickian, mobile-immobile behavior: INFORMATION CONTENT OF PUSH-PULL TESTS

Path reversibility and radial symmetry are often assumed in push-pull tracer test analysis. In reality, heterogeneous flow fields mean that both assumptions are idealizations. In this paper, to understand their impact, we perform a parametric study which quantifies the scattering effects of ambient flow, local-scale dispersion, and velocity field heterogeneity on push-pull breakthrough curves and compares them to the effects of mobile-immobile mass transfer (MIMT) processes including sorption and diffusion into secondary porosity. We identify specific circumstances in which MIMT overwhelmingly determines the breakthrough curve, which may then be considered uninformative about drift and local-scale dispersion. Assuming path reversibility, we develop a continuous-time-random-walk-based interpretation framework which is flow-field-agnostic and well suited to quantifying MIMT. Adopting this perspective, we show that the radial flow assumption is often harmless: to the extent that solute paths are reversible, the breakthrough curve is uninformative about velocity field heterogeneity. Our interpretation method determines a mapping function (i.e., subordinator) from travel time in the absence of MIMT to travel time in its presence. A mathematical theory allowing this function to be directly “plugged into” an existing Laplace-domain transport model to incorporate MIMT is presented and demonstrated. Algorithms implementing the calibration are presented and applied to interpretation of data from a push-pull test performed in a heterogeneous environment. A successful four-parameter fit is obtained, of comparable fidelity to one obtained using a million-node 3-D numerical model. In conclusion, we demonstrate analytically and numerically how push-pull tests quantifying MIMT are sensitive to remobilization, but not immobilization, kinetics.

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗

Evolution of Tantalum Content During Vacuum Induction Melting and Electroslag Remelting of a Novel Martensitic Steel

The need for materials with superior thermal and mechanical properties while mitigating cost increases interest in new complex alloy compositions which brings challenges to manufacturing processes. In this investigation, vacuum induction melting (VIM) and electroslag remelting (ESR) of a novel tantalum (Ta)-containing martensitic steel was performed using standard industry practices at a laboratory scale. A 25 pct loss of Ta was measured from the VIM electrode to the ESR ingot using X-ray fluorescence. Several tools were used for broad characterization of the ingots, including LECO for chemistry analysis, scanning electron microscopy, and electron probe microanalysis for observation of the precipitate and inclusion phases post-VIM and post-ESR as well as computational modeling of the ESR process for the calculation of macrosegregation and inclusion travel. It was found that a significant amount of Ta{sub 2}O{sub 5} inclusions formed during VIM and were transferred to the slag during ESR. While ESR was particularly successful at decreasing the number density of inclusions by 95 pct, additional efforts are needed with regard to vacuum leak rate and purity of stock material when melting novel advanced steels.

36 MATERIALS SCIENCE↗