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At least 55 records · Page 3

Single-Turnover Variable Chlorophyll Fluorescence as a Tool for Assessing Phytoplankton Photosynthesis and Primary Productivity: Opportunities, Caveats and Recommendations

Phytoplankton photosynthetic physiology can be investigated through single-turnover variable chlorophyll fluorescence (ST-ChlF) approaches, which carry unique potential to autonomously collect data at high spatial and temporal resolution. Over the past decades, significant progress has been made in the development and application of ST-ChlF methods in aquatic ecosystems, and in the interpretation of the resulting observations. At the same time, however, an increasing number of sensor types, sampling protocols, and data processing algorithms have created confusion and uncertainty among potential users, with a growing divergence of practice among different research groups. In this review, we assist the existing and upcoming user community by providing an overview of current approaches and consensus recommendations for the use of ST-ChlF measurements to examine in-situ phytoplankton productivity and photo-physiology. We argue that a consistency of practice and adherence to basic operational and quality control standards is critical to ensuring data inter-comparability. Large datasets of inter-comparable and globally coherent ST-ChlF observations hold the potential to reveal large-scale patterns and trends in phytoplankton photo-physiology, photosynthetic rates and bottom-up controls on primary productivity. As such, they hold great potential to provide invaluable physiological observations on the scales relevant for the development and validation of ecosystem models and remote sensing algorithms.

variable chlorophyll fluorescence↗

Robotic and Crewed Mars Missions Increasing the Demand for Planetary Protection Technology Needs

Planetary protection (PP) policy seeks to avoid harmful contamination by limiting biological and relevant organic contamination from spacecraft as well as preventing adverse changes to Earth’s biosphere when extraterrestial samples are brought back to Earth. The PP policy at NASA was updated in 2021 (NPR 8715.24) and 2022 (NASA-STD-8719.27) to enable missions by expanding the decades old prescriptive requirements to allow for an option of adopting performance-based requirements that are objectivesdriven, risk-informed and case-assured. In parallel, the final PP knowledge gap workshop was completed representing the international consensus on the key areas to be considered in developing crew PP policy. These knowledge gaps focused on key technology development areas in 1) microbial and human health monitoring, 2) technical and operations needed for contamination control and 3) natural transport of contamination on Mars. As robotic missions start to implement performance-based approaches and research and technology efforts commence to inform crew policy the demand for data quality driven verification and validation in relevant space environments. Examples of the types of testing that is envisioned includes test as you fly validation and verification of decontamination systems in a relevant on-orbit and Mars environment, developing lethality curves of terrestrial organisms to further our understanding of the biocidal impacts of Mars and the space environment, and particle transport model validation and verification. Thus, the PP discipline has identified the need for groundbased space environments to perform preliminary testing as validation and verification of flight systems and to advance the technology readiness level prior to further testing on-orbit or lunar environments to prepare for Mars.

J. Nick Benardini↗

Robotic and Crewed Mars Missions Increasing the Demand for Planetary Protection Technology Needs

Planetary protection (PP) policy seeks to avoid harmful contamination by limiting biological and relevant organic contamination from spacecraft as well as preventing adverse changes to Earth’s biosphere when extraterrestrial samples are brought back to Earth. The PP policy at NASA was updated in 2021 (NPR 8715.24) and 2022 (NASA-STD-8719.27) to enable missions by expanding the decades old prescriptive requirements to allow for an option of adopting performance-based requirements that are objectives-driven, risk-informed and case-assured. In parallel, the final PP knowledge gap workshop was completed representing the international consensus on the key areas to be considered in developing crew PP policy. These knowledge gaps focused on key technology development areas in 1) microbial and human health monitoring, 2) technical and operations needed for contamination control and 3) natural transport of contamination on Mars. As robotic missions start to implement performance-based approaches and research and technology efforts commence to inform crew policy the demand for data quality driven verification and validation in relevant space environments. Examples of the types of testing that is envisioned includes test as you fly validation and verification of decontamination systems in a relevant on-orbit and Mars environment, developing lethality curves of terrestrial organisms to further our understanding of the biocidal impacts of Mars and the space environment, and particle transport model validation and verification. Thus, the PP discipline has identified the need for ground-based space environments to perform preliminary testing as validation and verification of flight systems and to advance the technology readiness level prior to further testing on-orbit or lunar environments to prepare for Mars.

J Nick Benardini↗

NASA’s Planetary Protection Program to Assure Mission Safety and Success

NASA’s planetary protection program seeks to understand and control harmful contamination of solar systems targets of exploration by terrestrial contamination and prevent harmful biological contamination of the Earth-Moon system by extraterrestrial life, should it exist. To accomplish these objective’s NASA has developed a balanced safety and mission assurance strategy that leverages COSPAR Policy guidelines, workshops, scientific consensus, partnerships and international working groups to develop policy and implementation guidelines. Upcoming crewed missions to the Moon and Mars, as well as robotic missions to small solar system bodies, Europa, Titan and Mars are some of the driving activities of astrobiological interest that continue to emphasize the importance of planetary protection throughout the project life cycle. Development of a responsive and updated agency planetary protection policy has been a focus area in supporting upcoming mission opportunities for exploration to include Mars sample return and crewed mission concepts. An extensive update of this policy is underway which encompasses crewed and robotic procedural polices, a general technical requirements standard, and an implementation handbook. During this timeframe NASA has been working with the international community to develop scientific consensus, and to identify and fill in knowledge gaps for developing balanced policy guidelines, incorporation of risk informed decision making and quantitative technical standards. NASA has developed a planetary protection roadmap as a technology management strategy to track and monitor the development of each knowledge gaps. The Committee of Space Research (COSPAR) Policy on Planetary Protection and the National Academies of Science, Engineering, and Medicine’s Committee of Planetary Protection are used to inform updates to the planetary protection polices and guidelines. This integrated strategy for planetary protection seeks to provide a transparent, structured approach for enabling missions, providing guidance for NASA and NASA partnered missions, and being responsive to the increased interest and activities in space exploration whilst maintaining an understanding and control of harmful contamination.

James Benardini↗

NASA's Planetary Protection Program to Assure Mission Safety and Success

NASA's planetary protection program seeks to understand and control harmful contamination of solar systems targets of exploration by terrestrial contamination and prevent harmful biological contamination of the Earth-Moon system by extraterrestrial life, should it exist. To accomplish these objective's NASA has developed a balanced safety and mission assurance strategy that leverages COSPAR Policy guidelines, workshops, scientific consensus, partnerships and international working groups to develop policy and implementation guidelines. Upcoming crewed missions to the Moon and Mars, as well as robotic missions to small solar system bodies, Europa, Titan and Mars are some of the driving activities of astrobiological interest that continue to emphasize the importance of planetary protection throughout the project life cycle.Development of a responsive and updated agency planetary protection policy has been a focus area in supporting upcoming mission opportunities for exploration to include Mars sample return and crewed mission concepts. An extensive update of this policy is underway which encompasses crewed and robotic procedural polices, a general technical requirements standard, and an implementation handbook. During this timeframe NASA has been working with the international community to develop scientific consensus, and to identify and fill in knowledge gaps for developing balanced policy guidelines, incorporation of risk informed decision making and quantitative technical standards. NASA has developed a planetary protection roadmap as a technology management strategy to track and monitor the development of each knowledge gaps. The Committee of Space Research (COSPAR) Policy on Planetary Protection and the National Academies of Science, Engineering, and Medicine's Committee of Planetary Protection are used to inform updates to the planetary protection polices and guidelines. This integrated strategy for planetary protection seeks to provide a transparent, structured approach for enabling missions, providing guidance for NASA and NASA partnered missions, and being responsive to the increased interest and activities in space exploration whilst maintaining an understanding and control of harmful contamination.

Planetary Protection↗

Large Deployable Reflector (LDR) feasibility study update

In 1982 a workshop was held to refine the science rationale for large deployable reflectors (LDR) and develop technology requirements that support the science rationale. At the end of the workshop, a set of LDR consensus systems requirements was established. The subject study was undertaken to update the initial LDR study using the new systems requirements. The study included mirror materials selection and configuration, thermal analysis, structural concept definition and analysis, dynamic control analysis and recommendations for further study. The primary emphasis was on the dynamic controls requirements and the sophistication of the controls system needed to meet LDR performance goals.

Alff, W. H.↗

Investigating the Partitioning of Inorganic Elements Consumed by Humans between the Various Fractions of Human Wastes: An Alternative Approach

The elemental composition of food consumed by astronauts is well defined. The major elements carbon, hydrogen, oxygen, nitrogen and sulfur are taken up in large amounts and these are often associated with the organic fraction (carbohydrates, proteins, fats etc) of human tissue. On the other hand, a number of the elements are located in the extracellular fluids and can be accounted for in the liquid and solid waste fraction of humans. These elements fall into three major categories - cationic macroelements (e.g. Ca, K, Na, Mg and Si), anionic macroelements (e.g. P, S and Cl and 17 essential microelements, (e.g. Fe, Mn, Cr, Co, Cu, Zn, Se and Sr). When provided in the recommended concentrations to an adult healthy human, these elements should not normally accumulate in humans and will eventually be excreted in the different human wastes. Knowledge of the partitioning of these elements between the different human waste fractions is important in understanding (a) developing waste separation technologies, (b) decision-making on how these elements can be recovered for reuse in space habitats, and (c) to developing the processors for waste management. Though considerable literature exists on these elements, there is a lack of understanding and often conflicting data. Two major reasons for these problems include the lack of controlled experimental protocols and the inherently large variations between human subjects (Parker and Gallagher, 1988). We have used the existing knowledge of human nutrition and waste from the available literature and NASA documentation to build towards a consensus to typify and chemically characterize the various human wastes. It is our belief, that this could be a building block towards integrating a human life support and waste processing in a closed system.

Wignarajah, Kanapathipillai↗

Optimizing a Small RNAseq Analysis Pipeline for NASA GeneLab Using Open-Source Tools and Libraries

Small RNA sequencing (small RNAseq) is a powerful tool for studying the regulation of gene expression in various organisms. Small RNAseq has been leveraged in space biology research to study how expression of small RNAs, e.g. micro RNAs (miRNAs), small interfering RNAs (siRNAs), and piwi-interacting RNAs (piRNAs), change upon exposure to the space environment. NASA GeneLab currently hosts small RNAseq raw data derived from space-relevant experiments on the Open Science Data Repository (OSDR). To maximize the accessibility of these data to the scientific community, in addition to hosting raw data, which is only interpretable by bioinformaticians, GeneLab plans to process all small RNAseq datasets and make those processed data available to the scientific community via the OSDR. In this study, we present the development of the GeneLab standardized pipeline for processing small RNAseq datasets. Using human, plant, and synthetic small RNAseq datasets, we interrogate various open-source software and publicly available databases to evaluate their accuracy and reproducibility in each step of the pipeline. For quality control and adapter detection and trimming, we evaluated TrimGalore!, FASTX, SeqKit, and DNApi methods to optimize alignment to reference genomes. We compared BWA, Bowtie, and Bowtie2 to determine the optimal alignment tool. For each alignment tool we also assessed various reference databases, including Ensembl reference genomes and different types of small RNA reference databases, including genome, hairpin, and miRNA references from the miRbase and MirGeneDB databases. To quantify the aligned data, we compared SAMtools, HTSeq, and RSEM for counting alignment events from each alignment tool used. Finally, we evaluated various tools, including DESeq2 and EdgeR, for data normalization and subsequent differential expression analysis. We will present the results from our comparative analyses for each pipeline step and propose a consensus pipeline for processing small RNAseq data derived from various organisms exposed to the space environment.

SmallRNAseq, NASA GeneLab, quality control, adapte↗

An experiment with interactive planning models

Experiments on decision making in planning problems are described. Executives were tested in dealing with capital investments and competitive pricing decisions under conditions of uncertainty. A software package, the interactive risk analysis model system, was developed, and two controlled experiments were conducted. It is concluded that planning models can aid management, and predicted uses of the models are as a central tool, as an educational tool, to improve consistency in decision making, to improve communications, and as a tool for consensus decision making.

Beville, J.↗

The formation of the solar system - Consensus, alternatives, and missing factors

The current status on the theories of the solar-system formation is overviewed with emphasis placed on the principal concepts and processes involved. These processes include the formation of about 1 to 10 km diam planetesimals from the dust of the solar nebula; the physical processes that govern the interaction of these planetesimals with one another, which control their size and their velocity distribution; the circumstances that determine the way in which the planetesimals grow into planetary embryos; the processes that are likely to be important during the final stages of accumulation; and the possible origin of differences between the accumulation of the terrestrial planets, the giant planets, and the asteroids.

Wetherill, George W.↗

Recent Developments in Space Debris Mitigation Policy and Practices

In recent years, emphasis has shifted from national efforts to control the space debris population to international ones. Here, too, great progress has been made, most notably by the Inter-Agency Space Debris Coordination Committee (IADC) and the Committee on the Peaceful Uses of Outer Space (COPUOS) of the United Nations. Today, a firm international consensus is rapidly building on the principal space debris mitigation measures. The IADC is an association of the space agencies of ten countries (China, France, Germany, India, Italy, Japan, Russia, Ukraine, the United Kingdom, and the United States) and the European Space Agency, representing 17 countries of which four (France, Germany, Italy, and the United Kingdom) are also full IADC members. At the 17th meeting of the IADC in October 1999, a new Action Item (AI 17.2) was adopted to develop a set of consensus space debris mitigation guidelines. The purpose of the activity was to identify the most valuable space debris mitigation measures and to reach an international agreement on common directives. The IADC Space Debris Mitigation Guidelines (www.iadc-online.org/index.cgi?item=docs_pub) were formally adopted in October 2002 during the Second World Space Congress in Houston, Texas. Two years later a companion document, entitled Support to the IADC Space Debris Mitigation Guidelines, was completed to provide background and clarification for the guidelines.

Johnson, Nicholas L.↗

Environmental Stress Testing of the Single Sample Cylinder: A Proven Consensus Standard for Internal Gas Analysis (IGA) or Residual Gas Analysis (RGA)

In August 2008, Schuessler Consulting was contracted by NASA GSFC in support of the NASA Electronic Parts and Packaging (NEPP) program to perform two separate studies on moisture laden air in a stainless steel cylinder that had been designed to become a consensus standard for Test Method 1018. This Test Method was originally released for hybrids under Mil. Std. 883 but was quickly utilized on other microelectronic devices under the auspice of Mil. Std. 750. The cylinder had subsequently been fabricated for the 750 community. It was back-filled with moist air and subsequently analyzed over a period of time under a previous NASA contract. It had been shown that moisture in the 4000 - 5000 ppm range could be analyzed rather precisely with a mass spectrometer, commonly referred to as a Residual Gas Analyzer (RGA). The scope of this study was to ascertain if the composition and precision varied as a function of thermal shock at sub-zero temperatures and whether there was consensus when the standard was submitted to other RGA units. It was demonstrated and published that the consensus standard would yield precise RGA data for moisture within +/- 1% when optimized for a given RGA unit. It has been subsequently shown in this study at Oneida Research Services, that sub-zero storage did not affect that precision when a well-defined protocol for the analysis was followed. The consensus standard was taken to a second facility for analysis where it was found that moisture adsorption on the transfer lines caused precision to drop to +/- 12%. The Single Sample Cylinder (SSC) is a one liter stainless steel cylinder with associated sampling valves and has considerable weight and volume. But this considerable size allows for approximately 300 gas samples of the same composition to be delivered to any RGA unit. Lastly, a smaller cylinder, approximately 75 cc, of a second consensus standard was fabricated and tested with a different mix of fixed gases where moisture was kept in the 100 ppm range. This second standard has the potential of providing 30 gaseous samples and can be readily shipped to any analytical facility that desires to generate comparison RGA data. A series of comparison residual gas analyses was performed at the Honeywell Federal Manufacturing & Technologies facility in the National Nuclear Facility Administration s plant in Kansas City to complete this project. It was shown that improvements in the precision of a given RGA unit can be done by controlling the cycle time for each analysis and increasing analysis temperatures to minimize moisture adsorption. It was also found that a "one time event" in the subzero storage of the large SSC did not effect the units ability to continuously supply precise samples of the same chemistry, however the "event" caused a permanent +8% shift in the reported value of the moisture content. Lastly, a set of SSC RGA results was plotted on a common graph with DSCC "correlation study" RGA data. The result demonstrates the ability of the SSC to remove many of the individual variances that single, individual samples introduce. The consensus standards are now in storage at Oneida Research Services, one of the DSCC certified houses that does RGA to Military Standards, where they await future studies. The analytical data and the operational parameters of the instruments used are provided in the following discussion. Limitations and suggested means for improvement of both precision and accuracy are provided.

Schuessler, Philipp WH↗

Summary of studies for a solar optical telescope in space: 1968-1976

The primary objective of this review is to tabulate the basic recommendations of several solar telescope studies. A primary matrix, listing some of the basic optical parameters, was compiled and forms the basis for a table. From this table it is apparent that a strong consensus exists on the configuration of the telescope and on its fundamental dimensionless parameters. Other tables presented in this document address the basic approach of each study to the telescope design as well as to the design of critical subsystems. These subsystem problems include the material, coating, configuration, mounting, launch locks, and thermal control of the primary mirror, the structure of the main telescope and the instrument bay, the mechanisms for radiation rejection, thermal control, and meteoroid shielding, and methods of maintaining image quality by proper alignment and by image motion compensation.

Bremer, J.↗

Beyond Solar-B: MTRAP, the Magnetic Transition Region Probe

The next generation of solar missions will reveal and measure fine-scale solar magnetic fields and their effects in the solar atmosphere at heights, small scales, sensitivities, and fields of view well beyond the reach of Solar-B. The necessity for, and potential of, such observations for understanding solar magnetic fields, their generation in and below the photosphere, and their control of the solar atmosphere and heliosphere, were the focus of a science definition workshop, 'High-Resolution Solar Magnetography from Space: Beyond Solar-B,' held in Huntsville Alabama in April 2001. Forty internationally prominent scientists active in solar research involving fine-scale solar magnetism participated in this Workshop and reached consensus that the key science objective to be pursued beyond Solar-B is a physical understanding of the fine-scale magnetic structure and activity in the magnetic transition region, defined as the region between the photosphere and corona where neither the plasma nor the magnetic field strongly dominates the other. The observational objective requires high cadence (less than 10s) vector magnetic field maps, and spatially resolved spectra from the IR, visible, vacuum UV, to the EUV at high resolution (less than 50km) over a large FOV (approximately 140,000 km). A polarimetric resolution of one part in ten thousand is required to measure transverse magnetic fields of less than 30G. The latest SEC Roadmap includes a mission identified as MTRAP to meet these requirements. Enabling technology development requirements include large, lightweight, reflecting optics, large format sensors (16K x 16K pixels) with high QE at 150 nm, and extendable spacecraft structures. The Science Organizing Committee of the Beyond Solar-B Workshop recommends that: (1) Science and Technology Definition Teams should be established in FY04 to finalize the science requirements and to define technology development efforts needed to ensure the practicality of MTRAP's observational goals; (2) The necessary technology development funding should be included in Code S budgets for FY06 and beyond to prepare MTRAP for a new start no later than the nominal end of the Solar-B mission, around 2010.

Davis, John M.↗

Development of a New Departure Aversion Standard for Light Aircraft

The Federal Aviation Administration (FAA) and European Aviation Safety Agency (EASA) have recently established new light aircraft certification rules that introduce significant changes to the current regulations. The changes include moving from prescriptive design requirements to performance-based standards, transferring many of the acceptable means of compliance out of the rules and into consensus standards. In addition, the FAA/EASA rules change the performance requirements associated with some of the more salient safety issues regarding light aircraft. One significant change is the elimination of spin recovery demonstration. The new rules now call for enhanced stall warning and aircraft handling characteristics that demonstrate resistance to inadvertent departure from controlled flight. The means of compliance with these changes in a safe, cost-effective manner is a challenging problem. This paper discusses existing approaches to reducing the likelihood of departure from controlled flight and introduces a new approach, dubbed Departure Aversion, which allows applicants to tailor the amount of departure resistance, stall warning, and enhanced safety equipment to meet the new proposed rules. The Departure Aversion approach gives applicants the freedom to select the most cost-effective portfolio for their design, while meeting the safety intent of the new rules, by ensuring that any combination of the selected approaches will be at a higher equivalent level of safety than today's status quo.

Borer, Nicholas K.↗

An Updated TRMM Composite Climatology of Tropical Rainfall and Its Validation

An updated 15-yr Tropical Rainfall Measuring Mission (TRMM) composite climatology (TCC) is presented and evaluated. This climatology is based on a combination of individual rainfall estimates made with data from the primaryTRMMinstruments: theTRMM Microwave Imager (TMI) and the precipitation radar (PR). This combination climatology of passive microwave retrievals, radar-based retrievals, and an algorithm using both instruments simultaneously provides a consensus TRMM-based estimate of mean precipitation. The dispersion of the three estimates, as indicated by the standard deviation sigma among the estimates, is presented as a measure of confidence in the final estimate and as an estimate of the uncertainty thereof. The procedures utilized by the compositing technique, including adjustments and quality-control measures, are described. The results give a mean value of the TCC of 4.3mm day(exp -1) for the deep tropical ocean beltbetween 10 deg N and 10 deg S, with lower values outside that band. In general, the TCC values confirm ocean estimates from the Global Precipitation Climatology Project (GPCP) analysis, which is based on passive microwave results adjusted for sampling by infrared-based estimates. The pattern of uncertainty estimates shown by sigma is seen to be useful to indicate variations in confidence. Examples include differences between the eastern and western portions of the Pacific Ocean and high values in coastal and mountainous areas. Comparison of the TCC values (and the input products) to gauge analyses over land indicates the value of the radar-based estimates (small biases) and the limitations of the passive microwave algorithm (relatively large biases). Comparison with surface gauge information from western Pacific Ocean atolls shows a negative bias (~16%) for all the TRMM products, although the representativeness of the atoll gauges of open-ocean rainfall is still in question.

(GPCP) Global Precipitation Project analysis↗

The Space Communications Protocol Standards Program

In the fall of 1992 NASA and the Department of Defense chartered a technical team to explore the possibility of developing a common set of space data communications standards for potential dual-use across the U.S. national space mission support infrastructure. The team focused on the data communications needs of those activities associated with on-lined control of civil and military aircraft. A two-pronged approach was adopted: a top-down survey of representative civil and military space data communications requirements was conducted; and a bottom-up analysis of available standard data communications protocols was performed. A striking intersection of civil and military space mission requirements emerged, and an equally striking consensus on the approach towards joint civil and military space protocol development was reached. The team concluded that wide segments of the U.S. civil and military space communities have common needs for: (1) an efficient file transfer protocol; (2) various flavors of underlying data transport service; (3) an optional data protection mechanism to assure end-to-end security of message exchange; and (4) an efficient internetworking protocol. These recommendations led to initiating a program to develop a suite of protocols based on these findings. This paper describes the current status of this program.

Jeffries, Alan↗

Second Generation Reusable Launch Vehicle Development and Global Competitiveness of US Space Transportation Industry: Critical Success Factors Assessment

In response to the unrelenting call in both public and private sectors fora to reduce the high cost associated with space transportation, many innovative partially or fully RLV designs (X-34-37) were initiated. This call is directed at all levels of space missions including scientific, military, and commercial and all aspects of the missions such as nonrecurring development, manufacture, launch, and operations. According to Wertz (2000), over thirty years, the cost of space access has remained exceedingly high. For example, London (1996, 1994), Wertz (1996) and Hammond (1999) have discussed in details the reasons for the skyrocket launch costs. The consensus in the popular press is that to decrease the current astronomical cost of access to space, more safer, reliable, and economically viable SGRLVs must be developed. This current skyrocket cost of space launch poses a major constraint to broadening and expanding U.S. economic activities in LEO. As a result, affecting global competitiveness of U. S. CST industry. Foreign firms control greater share of the launch market once dominated by U.S. For example, in the mid 1980s U.S. service providers had approximately 100% of the commercial space launch market revenues, but today U.S. has less than 50% of that market (Graham, 2001). Further, because of the growth potential in the international launch market, countries such as Brazil, India, Japan and Israel are gearing up to enter the global launch market with their own commercial space launch vehicles. Considering this fact, NASA and U.S. space launch industry cannot afford to lag behind. Therefore developing SGRLVs will immeasurably improve the U.S. space transportation capabilities by helping the U.S. to regain the global commercial space markets while supporting the transportation capabilities of NASA's space mission.

Chris I. Enyinda↗