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All sourcesNASA NTRSDOE OSTI
NASA NTRS1992

Purification and Properties of an ATPase from Sulfolobus solfataricus

A sulfite-activated ATPase isolated from Sulfolobus solfataricus had a relative molecular mass of 370,000. It was composed of three subunits whose relative molecular masses were 63,000, 48,000, and 24,000. The enzyme was inhibited by the vacuolar ATPase inhibitors nitrate and N-ethylmaleimide; 4-chloro-7-nitrobenzo-furazan (NBD-Cl) was also inhibitory. N-Ethylmaleimide was predominately bound to the largest subunit while NBD-CL was bound to both subunits. ATPase activity was inhibited by low concentrations of p-chloromercuri-phenyl sulfonate and the inhibition was reversed by cysteine which suggested that thiol groups were essential for activity. While the ATPase from S. solfataricus shared several properties with the ATPase from S. acidocaldarius there were significant differences. The latter enzyme was activated by sulfate and chloride and was unaffected by N-ethylmaleimide, whereas the S. solfataricus ATPase was inhibited by these anions as well as N-ethyimaleimide. These differences as well as differences that occur in other vacuolar-like ATPases isolated from the methanogenic and the extremely halophilic bacteria suggest the existence of several types of archaeal ATPases, none of which have been demonstrated to synthesize ATP.

Hochstein, Lawrence I.↗
NASA NTRS1988

Ionic binding properties of carrier ampholytes

Radioactive ampholytes were synthesized with specific activity of 638 microCi/g. These were used in studies of ampholyte binding to target proteins under nonionic conditions. These radioactive ampholytes bound to target proteins but were dissociable in sodium chloride solutions with dissociation occurring in a concentration dependent way. The ampholytes could be dissociated from target molecules using excess unlabelled ampholytes synthesized in the laboratory as well as commercial ampholytes. Radioactive ampholytes were bound to target proteins with different isoelectric points, and the bound ampholytes were eluted and analyzed by recycling isoelectric focusing. The results showed that acidic proteins bound basic ampholytes and basic proteins bound acidic ampholytes.

Rodkey, L. Scott↗
NASA NTRS1993

Regenerable biocide delivery unit

A method and apparatus are disclosed for maintaining continuous, long-term microbial control in the water supply for potable, hygiene, and experimental water for space activities, as well as treatment of water supplies on Earth. The water purification is accomplished by introduction of molecular iodine into the water supply to impart a desired iodine residual. The water is passed through an iodinated anion exchange resin bed. The iodine is bound as I-(sub n) at the anion exchange sites and releases I(sub 2) into the water stream flowing through the bed. The concentration of I(sub 2) in the flowing water gradually decreases and, in the prior art, the ion-exchange bed has had to be replaced. In a preferred embodiment, a bed of iodine crystals is provided with connections for flowing water therethrough to produce a concentrated (substantially saturated) aqueous iodine solution which is passed through the iodinated resin bed to recharge the bed with bound iodine. The bed of iodine crystals is connected in parallel with the iodinated resin bed and is activated periodically (e.g., by timer, by measured flow of water, or by iodine residual level) to recharge the bed. Novelty resides in the capability of inexpensively and repeatedly regenerating the ion-exchange bed in situ.

Sauer, Richard L.↗
NASA NTRS1998

Inner Core Rotation from Geomagnetic Westward Drift and a Stationary Spherical Vortex in Earth's Core

The idea that geomagnetic westward drift indicates convective leveling of the planetary momentum gradient within Earth's core is pursued in search of a differentially rotating mean state, upon which various oscillations and secular effects might be superimposed. The desired state conforms to roughly spherical boundary conditions, minimizes dissipative interference with convective cooling in the bulk of the core, yet may aid core cooling by depositing heat in the uppermost core and lower mantle. The variational calculus of stationary dissipation applied to a spherical vortex within the core yields an interesting differential rotation profile, akin to spherical Couette flow bounded by thin Hartmann layers. Four boundary conditions are required. To concentrate shear induced dissipation near the core-mantle boundary, these are taken to be: (i) no-slip at the core-mantle interface; (ii) geomagnetically estimated bulk westward flow at the base of the core-mantle boundary layer; (iii) no-slip at the inner-outer core interface; and, to describe magnetic locking of the inner core to the deep outer core; (iv) hydrodynamically stress-free at the inner-outer core boundary. By boldly assuming the axial core angular momentum anomaly to be zero, the super-rotation of the inner core relative to the mantle is calculated to be at most 1.5 deg./yr.

Voorhies, Coerte V.↗
NASA NTRS1999

Inner Core Rotation from Geomagnetic Westward Drift and a Stationary Spherical Vortex in Earth's Core

The idea that geomagnetic westward drift indicates convective leveling of the planetary momentum gradient within Earth's core is pursued in search of a differentially rotating mean state, upon which various oscillations and secular effects might be superimposed. The desired state conforms to roughly spherical boundary conditions, minimizes dissipative interference with convective cooling in the bulk of the core, yet may aide core cooling by depositing heat in the uppermost core and lower mantle. The variational calculus of stationary dissipation applied to a spherical vortex within the core yields an interesting differential rotation profile akin to spherical Couette flow bounded by thin Hartmann layers. Four boundary conditions are required. To concentrate shear induced dissipation near the core-mantle boundary, these are taken to be: (i) no-slip at the core-mantle interface; (ii) geomagnetically estimated bulk westward flow at the base of the core-mantle boundary layer; (iii) no-slip at the inner-outer core interface; and, to describe magnetic locking of the inner core to the deep outer core, (iv) hydrodynamically stress-free at the inner-outer core boundary. By boldly assuming the axial core angular momentum anomaly to be zero, the super-rotation of the inner core is calculated to be at most 1.5 degrees per year.

Voorhies, C. V.↗
NASA NTRS1982

Apollo lunar seismic experiment - Final summary

Processing and initial analysis of the entire set of Apollo lunar seismic data collected continuously from 1969 through 1977 have now been completed. Recent results include: (1) better defined deep moonquake locations, which appear to be bounded rather sharply between about 800 km and 1000 km depths with concentrations near both boundaries; and (2) middle mantle (approximately 500 to 1000 km depth) seismic velocities of V sub p = 8.3 + or - 0.4 km/sec and V sub s = 4.6 + or - 0.2 km/sec, which are significantly higher than previous estimates and represent an increase of velocities from the upper mantle as opposed to a decrease in previous estimates.

Nakamura, Y.↗
NASA NTRS1986

A comparison of the regional slope characteristics of Venus and earth - Implications for geologic processes on Venus

The range of 3 degree by 3 degree regional slopes of the Earth and Venus is similar (approximately 0.0-2.4 degrees), although the surface distribution of these values differs significantly. On earth, cratonic and abyssal plains form extensive regions of 0.0 degree slope. Within these regions a variety of features (mid-ocean ridges, volcanic island chains, subduction zones, and floded mountains) have regional slope characteristics influenced by seafloor spreading and plate recycling, as well as an active weathering regime. The plains provinces of Venus are much more rugged than earth's plains and are marked by numerous closely spaced circular and linear features (0.1-0.2 degree regional slope) concentrated into broad linear zones of global extent. Although Venus highlands are bounded by narrow zones of relatively steep slope, the margins of Aphrodite Terra and Beta Regio are not as steep as earth's continental margins and appear to be best developed parallel to the trends of major chasmata within these regions. Ishtar Terra's margins are significantly steeper and more continuous than other highland margins and are comparable to passive margins on earth. The Venus highlands do not contain appreciable smooth, flat interior regions, implying that highland topography is not significantly modified by erosion or deposition.

Sharpton, V. L.↗
NASA NTRS1998

Making the Impossible Possible: Strategies for Fast POMDP Monitoring

Systems modeled as partially observable Markov decision processes (POMDPs) can be tracked quickly with three restrictions: all actions are grouped together, the out-degree of each system state is bounded by a constant, and the number of non-zero elements in the belief state is bounded by a (different) constant. With these restrictions, the tracking algorithm operates in constant time and linear space. The first restriction assumes that the action itself is unobservable. The second restriction defines a subclass of POMDPs that covers however a wide range of problems. The third restriction is an approximation technique that can lead to a potentially vexing problem: an observation may be received that has probability according to the restricted belief state. This problem of impossibility will cause the belief state to collapse. In this paper we discuss the tradeoffs between the constant bound on the belief state and the quality of the solution. We concentrate on strategies for overcoming the impossibility problem and demonstrate initial experimental results that indicate promising directions.

Washington, Richard↗
NASA NTRS2002

Distinguishing Indigenous from Contaminating Microorganisms in Rock Samples from a Deep Au Mine in South Africa

The concentration and distribution of microbial biomass within deep subsurface rock strata is not well known To date, most analyses are from water samples and a few cores. Hand samples, block samples and cores from an actively mined Carbon Leader ore zone at 3.2 kilometers depth were collected for microbial analyses. The Carbon Leader was comprised of quartz, S-bearing aromatic hydrocarbons, Fe(III) oxyhydroxides, sulfides, uraninite, Au and minor amounts of sulfate. The porosity of the ore was 1% and the maximum pore throat diameter was less than 0.1 microns; whereas, the porosity of the adjacent quartzite was .02 to .9% with a maximum pore throat diameter of 0.9 microns. Rhodamine dye, fluorescent microspheres, microbial enrichments, autoradiography, phospholipid fatty acid (PLEA) and 16S rDNA analyses were performed on these rock samples and the mining water. The date indicate that the levels of solute contamination less than 0.01% for pared rock samples. Despite this low level of contamination, PLEA, microbial enrichment, DNA and tracer analyses and calculations indicate that most of the viable microorganisms in the Carbon Leader represent gram negative aerobic heterotrophs and ammonia oxidizers that are phylogenetically identical or closely related to service water microorganisms. These microbial contaminants probably infiltrated the low permeability rock through mining-induced microfractures. Geochemical data also detected drilling water in a fault zone approx. 1 meter behind the rock face encountered during coring. The mining induced macrofractures that are common at these great depths act as pathways for the drilling water borne microorganisms into the lower temperature zone that extends several meters into rock strata from the rock face. Combined PLEA and T- RFLP analyses of the service water and Carbon Leader samples indicate that the concentration of indigenous microorganisms was less than 10(exp 2) cells/gram. Such a low concentrations result from the submicron pore throat diameters. PLFA. SO4-35 autoradiography and tracer analyses indicate that the bounding quartzite contains thermophilic sulfate reducing bacteria at 10(exp 3) cells/gram that are not attributable to drilling water contamination. The microorganisms may be surviving on sulfate generated by oxidation of sulfide by radiolytic reactions resulting from the high U concentration in the ore zone. The presence of up to 8,000 ppm of Fe(III) oxyhydroxides in the host rock will also act to recycle sulfide generated by the sulfate reducing bacteria into sulfate. The activity of these sulfate-reducing bacteria may be enhanced by mining induced fracturing which can propagate up to 40 meters into virgin rock where the temperatures are ca. 50 C, and decrepitate of sulfate rich fluid inclusions. In ultra deep mines, judicious application of tracers and multiple microbial characterization techniques can distinguish microbial contamination caused by the near field fracturing and drilling water migration from the indigenous microbial communities in rock strata. The importance of far field fracturing on indigenous microbial communities, however, remains unknown.

Onstott, T. C.↗
NASA NTRS2009

Distillation and Air Stripping Designs for the Lunar Surface

Air stripping and distillation are two different gravity-based methods, which may be applied to the purification of wastewater on the lunar base. These gravity-based solutions to water processing are robust physical separation techniques, which may be advantageous to many other techniques for their simplicity in design and operation. The two techniques can be used in conjunction with each other to obtain high purity water. The components and feed compositions for modeling waste water streams are presented in conjunction with the Aspen property system for traditional stage distillation models and air stripping models. While the individual components for each of the waste streams will vary naturally within certain bounds, an analog model for waste water processing is suggested based on typical concentration ranges for these components. Target purity levels for the for recycled water are determined for each individual component based on NASA s required maximum contaminant levels for potable water Distillation processes are modeled separately and in tandem with air stripping to demonstrate the potential effectiveness and utility of these methods in recycling wastewater on the Moon. Optimum parameters such as reflux ratio, feed stage location, and processing rates are determined with respect to the power consumption of the process. Multistage distillation is evaluated for components in wastewater to determine the minimum number of stages necessary for each of 65 components in humidity condensate and urine wastewater mixed streams. Components of the wastewater streams are ranked by Henry s Law Constant and the suitability of air stripping in the purification of wastewater in terms of component removal is evaluated. Scaling factors for distillation and air stripping columns are presented to account for the difference in the lunar gravitation environment. Commercially available distillation and air stripping units which are considered suitable for Exploration Life Support are presented. The advantages to the various designs are summarized with respect to water purity levels, power consumption, and processing rates.

Boul, Peter J.↗
NASA NTRS2010

Distillation Designs for the Lunar Surface

Gravity-based distillation methods may be applied to the purification of wastewater on the lunar base. These solutions to water processing are robust physical separation techniques, which may be more advantageous than many other techniques for their simplicity in design and operation. The two techniques can be used in conjunction with each other to obtain high purity water. The components and feed compositions for modeling waste water streams are presented in conjunction with the Aspen property system for traditional stage distillation. While the individual components for each of the waste streams will vary naturally within certain bounds, an analog model for waste water processing is suggested based on typical concentration ranges for these components. Target purity levels for recycled water are determined for each individual component based on NASA s required maximum contaminant levels for potable water Optimum parameters such as reflux ratio, feed stage location, and processing rates are determined with respect to the power consumption of the process. Multistage distillation is evaluated for components in wastewater to determine the minimum number of stages necessary for each of 65 components in humidity condensate and urine wastewater mixed streams.

Boul, Peter J.↗
NASA NTRS1990

Atmospheric carbon monoxide - Latitudinal distribution of sources

Global measurements of CO, taken over 2-8 years at 16 sites, are used to estimate the latitudinal and seasonal distributions of the sources. It is found that the most concentrated sources of CO, amounting to some 30 percent of the global production, are in the industrialized regions bounded by 30 and 50 deg N. About 47 percent of CO comes from the whole of the midnorthern latitudes and another 40 percent comes from the tropics. The calculations show that the production of CO is significantly higher during spring and summer, particularly in the industrialized latitudes. From these results it is apparent that the observed seasonal cycle of CO concentrations cannot be explained by the seasonal variation of OH alone.

Khalil, M. A. K.↗
NASA NTRS1997

NGC 5291: Implications for the Formation of Dwarf Galaxies

The possible formation and evolution of dwarf irregular galaxies from material derived from perturbed evolved galaxies is addressed via an H I study of a likely example, the peculiar system NGC 5291. This system, located in the western outskirts of the cluster Abell 3574, contains the lenticular galaxy NGC 5291 which is in close proximity to a disturbed companion and is flanked by an extensive complex of numerous knots extending roughly 4 min north and 4 min south of the galaxy. In an initial optical and radio study, Longmore et al. (1979, MNRAS, 188, 285) showed that these knots have the spectra of vigorous star-forming regions, and suggested that some may in fact be young dwarf irregular galaxies. High resolution 21-cm line observations taken with the VLA are presented here and reveal that the H I distribution associated with this system encompasses not only the entire N-S complex of optical knots, but also forms an incomplete ring or tail that extends approximately 3 min to the west. The H I associated with NGC 5291 itself shows a high velocity range; the Seashell is not detected. The formation mechanism for this unusual system is unclear and two models - a large, low-luminosity ram-swept disk, and a ram-swept interaction-are discussed. The H I in the system contains numerous concentrations, mostly along the N-S arc of the star-forming complexes, which generally coincide with one or more optical knots; the larger H I features contain several x 10(exp 9) solar mass of gas. Each of the knots is compared to a set of criteria designed to determine if these objects are bound against their own internal kinetic energy and are tidally stable relative to the host galaxy. An analysis of the properties of the H I concentrations surrounding the optical star-forming complexes indicates that at least the largest of these is a bound system; it also possesses a stellar component. It is suggested that this object is a genuinely young dwarf irregular galaxy that has evolved from the material associated with the system and that this entire complex contains several proto- or young dwarf irregular galaxies in various stages of development. We are therefore witnessing the early evolution of a number of genuinely young galaxies. Given the evident importance of the NGC 5291 system as a 'nursery' for young galaxies, careful modeling is required if we are to understand this remarkable galaxy.

Malphrus, Benjamin K.↗
NASA NTRS1995

Competition of supermassive black holes and galactic spheroids in the destruction of globular clusters

The globular clusters that we observe in galaxies may be only a fraction of the initial population. Among the evolutionary influences on the population is the destruction of globular clusters by tidal forces as the cluster moves through the field of influence of a disk, a bulge, and/or a putative nuclear component (black hole). We have conducted a series of N-body simulations of globular clusters on bound and marginally bound orbits through poetentials that include black hole and speroidal components. The degree of concentration of the spheroidal component can have a considerable impact on the extent to which a globular cluster is disrupted. If half the mass of a 10(exp 10) solar mass spheroid is concentrated within 800 pc, then only black holes with masses greater than 10(exp 9) solar mass can have a significant tidal influence over that already exerted by the bulge. However, if the matter in the spheroidal component is not so strongly concentrated toward the center of the galaxy, a more modest central black hole (down to 10(exp 8) solar mass) could have a dominant influence on the globular cluster distribution, particularly if many of the clusters were initially on highly radial orbits. Our simulations show that the stars that are stripped from a globular cluster follow orbits with roughly the same eccentricity as the initial cluster orbit, spreading out along the orbit like a 'string of pearls.' Since only clusters on close to radial orbits will suffer substantial disruption, the population of stripped stars will be on orbits of high eccentricity.

Charlton, Jane C.↗
NASA NTRS2005

A First and Second Order Moment Approach to Probabilistic Control Synthesis

This paper presents a robust control design methodology based on the estimation of the first two order moments of the random variables and processes that describe the controlled response. Synthesis is performed by solving an multi-objective optimization problem where stability and performance requirements in time- and frequency domains are integrated. The use of the first two order moments allows for the efficient estimation of the cost function thus for a faster synthesis algorithm. While reliability requirements are taken into account by using bounds to failure probabilities, requirements related to undesirable variability are implemented by quantifying the concentration of the random outcome about a deterministic target. The Hammersley Sequence Sampling and the First- and Second-Moment- Second-Order approximations are used to estimate the moments, whose accuracy and associated computational complexity are compared numerically. Examples using output-feedback and full-state feedback with state estimation are used to demonstrate the ideas proposed.

Crespo, Luis G.↗
NASA NTRS2022

Global geologic map of asteroid (101955) Bennu indicates heterogeneous resurfacing in the past 500,000 years

Global geologic maps are useful tools for efficient interpretation of a planetary body, and they provide global context for the diversity and evolution of the surface. We used data acquired by the OSIRIS-REx spacecraft to create the first global geologic map of the near-Earth asteroid (101955) Bennu. As this is the first geologic map of a small, non-spherical, rubble-pile asteroid, we discuss the distinctive mapping challenges and best practices that may be useful for future exploration of similar asteroids, such as those to be visited with the Hera and Janus missions. By mapping on two centimeter-scale global image mosaics (2D projected space) and a centimeter-scale global shape model (3D space), we generated three input maps respectively describing Bennu’s shape features, geologic features, and surface texture. Based on these input maps, we defined two geologic units: the Smooth Unit and the Rugged Unit. The units are differentiated primarily on the basis of surface texture, concentrations of boulders, and the distributions of lineaments, mass movement features, and craters. They are bounded by several scarps. The Rugged Unit contains abundant boulders and signs of recent mass movement. It also has fewer small(<20 m), putatively fresh craters than the Smooth Unit, suggesting that such craters have been erased in the former. Based on these geologic indicators, we infer that the Rugged Unit has the younger surface of the two. Differential crater size-frequency distributions and the distribution of the freshest craters suggest that both unit surfaces formed ~10–65 million years ago, when Bennu was located in the Main Asteroid Belt, and the Smooth Unit has not been significantly resurfaced in the past 2 million years. Meanwhile, the Rugged Unit has experienced resurfacing within the past ~500,000years during Bennu’s lifetime as a near-Earth asteroid. The geologic units are consistent with global diversity in slope, surface roughness, normal albedo, and thermal emission spectral characteristics. The site on Bennu where the OSIRIS-REx mission collected a regolith sample is located in the Smooth Unit, in a small crater nested within a larger one. So although the Smooth Unit is an older surface than the Rugged Unit, the impact-crater settingindicates that the material sampled was recently exposed. Several similarities are apparent between Bennu and asteroid (162173) Ryugu from a global geologic perspective, including two geologic units distinguishable by variations in the number density of boulders, as well as in other datasets such as brightness.

E R Jawin↗
NASA NTRS2000

The Impact of Sea Ice Concentration Accuracies on Climate Model Simulations with the GISS GCM

The Goddard Institute for Space Studies global climate model (GISS GCM) is used to examine the sensitivity of the simulated climate to sea ice concentration specifications in the type of simulation done in the Atmospheric Modeling Intercomparison Project (AMIP), with specified oceanic boundary conditions. Results show that sea ice concentration uncertainties of +/- 7% can affect simulated regional temperatures by more than 6 C, and biases in sea ice concentrations of +7% and -7% alter simulated annually averaged global surface air temperatures by -0.10 C and +0.17 C, respectively, over those in the control simulation. The resulting 0.27 C difference in simulated annual global surface air temperatures is reduced by a third, to 0.18 C, when considering instead biases of +4% and -4%. More broadly, least-squares fits through the temperature results of 17 simulations with ice concentration input changes ranging from increases of 50% versus the control simulation to decreases of 50% yield a yearly average global impact of 0.0107 C warming for every 1% ice concentration decrease, i.e., 1.07 C warming for the full +50% to -50% range. Regionally and on a monthly average basis, the differences can be far greater, especially in the polar regions, where wintertime contrasts between the +50% and -50% cases can exceed 30 C. However, few statistically significant effects are found outside the polar latitudes, and temperature effects over the non-polar oceans tend to be under 1 C, due in part to the specification of an unvarying annual cycle of sea surface temperatures. The +/- 7% and 14% results provide bounds on the impact (on GISS GCM simulations making use of satellite data) of satellite-derived ice concentration inaccuracies, +/- 7% being the current estimated average accuracy of satellite retrievals and +/- 4% being the anticipated improved average accuracy for upcoming satellite instruments. Results show that the impact on simulated temperatures of imposed ice concentration changes is least in summer, encouragingly the same season in which the satellite accuracies are thought to be worst. Hence the impact of satellite inaccuracies is probably less than the use of an annually averaged satellite inaccuracy would suggest.

Parkinson, Claire L.↗
NASA NTRS1982

The detailed chemistry and thermodynamics of sodium in oxygen-rich flames

Measurement of sodium and OH concentrations in ten oxygen-rich H2/O2/N2 flames by respective saturated and low-power laser induced fluorescence techniques have permitted a detailed examination of the pronounced flame chemistry of sodium in such oxygen rich media. Previous interpretations have been shown to be largely incomplete or in error. The flame downstream profiles indicate that the amount of free sodium tracks the decay of H-atom and as the flame radicals decay sodium becomes increasingly bound in a molecular form. A detailed kinetic model indicates that the sodium is distributed between NaOH and NaO2 species. Concentrations of NaO are very small and NaH negligible. The actual distribution is controlled by the state of equilibrium of the flames' basic free radicals. Na, NaO2 and NaOH are all coupled to one another by fast reactions which can rapidly interconvert one to another as flame conditions vary. Above about 2000K, NaOH becomes dominant whereas NaO2 plays an increasingly important contribution at lower temperatures.

Hynes, A. J.↗