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The significance of finding viable extreme halophiles in halites associated with Permian-aged sedimentary deposits is considered. Issues related to the microbiology and geochemistry of the halite environment are addressed. Recommendations that related the significance of this phenomenon to NASA's interest in planetary exploration and the early evolution of life are provided.
Over the past several months, major industry vendors have made a business case for the network computer as a win-win solution toward lowering total cost of ownership. This report provides results from Phase I of the Ames Research Center network computer evaluation project. It identifies factors to be considered for determining cost of ownership; further, it examines where, when, and how network computer technology might fit in NASA's desktop computing architecture.
When claims for the long-term survival of viable organisms are made, either within terrestrial minerals or on Mars, considerations should be made of the limitations imposed by the naturally occurring radiation dose to which they have been exposed. We investigated the effect of ionizing radiation on different bacterial spores by measuring the inactivation constants for B. subtilis and s. marismortui spores in solution as well as for dry spores of B. subtilis and B. thuringiensis. S. marismortui is a halophilic spore that is genetically similar to the recently discovered 2-9-3 bacterium from a halite fluid inclusion, claimed to be 250 million years old, B. thuringiensis is a soil bacterium that is genetically similar to the human pathogens B. anthracis and B. cereus. To relate the inactivation constant to some realistic environments, we calculated the radiation regimen in a halite fluid inclusion and in the Martian subsurface over time. Our conclusion is that the ionizing dose of radiation in those environments limits the survival of viable bacterial spores over long periods. In the absence of an active repair mechanism in the dormant state, the long-term survival of spores is limited to less than 109 million years in halite fluid inclusions, to 100 to 160 million years in the Martian subsurface below 3 m, and to less than 600,000 years in the upper-most meter of Mars.
Cereal leaves subjected to the osmotica routinely used for protoplast isolation show a rapid increase in arginine decarboxylase activity, a massive accumulation of putrescine, and slow conversion of putrescine to the higher polyamines, spermidine and spermine (HE Flores, AW Galston 1984 Plant Physiol 75: 102). Mesophyll protoplasts from these leaves, which have a high putrescine:polyamine ratio, do not undergo sustained division. By contrast, in Nicotiana, Capsicum, Datura, Trigonella, and Vigna, dicot genera that readily regenerate plants from mesophyll protoplasts, the response of leaves to osmotic stress is opposite to that in cereals. Putrescine titer as well as arginine and ornithine decarboxylase activities decline in these osmotically stressed dicot leaves, while spermidine and spermine titers increase. Thus, the putrescine:polyamine ratio in Vigna protoplasts, which divide readily, is 4-fold lower than in oat protoplasts, which divide poorly. We suggest that this differing response of polyamine metabolism to osmotic stress may account in part for the failure of cereal mesophyll protoplasts to develop readily in vitro.
A suite of fluorescent intracellular stains and probes was used, in conjunction with viable plate counts, to assess the effect of chlorine disinfection on membrane potential (rhodamine 123; Rh123 and bis-(1,3-dibutylbarbituric acid) trimethine oxonol; DiBAC4(3)), membrane integrity (LIVE/DEAD BacLight kit), respiratory activity (5-cyano-2,3-ditolyl tetrazolium chloride; CTC) and substrate responsiveness (direct viable counts; DVC) in the commensal pathogen Escherichia coli O157:H7. After a 5 min exposure to the disinfectant, physiological indices were affected in the following order: viable plate counts > substrate responsiveness > membrane potential > respiratory activity > membrane integrity. In situ assessment of physiological activity by examining multiple targets, as demonstrated in this study, permits a more comprehensive determination of the site and extent of injury in bacterial cells following sublethal disinfection with chlorine. This approach to assessing altered bacterial physiology has application in various fields where detection of stressed bacteria is of interest.
An effective simulation of an object, process, or task must be similar to that object, process, or task. A simulation could consist of a physical device, a set of mathematical equations, a computer program, a person, or some combination of these. There are many reasons for the use of simulators. Although some of the reasons are unique to a specific situation, there are many general reasons and purposes for using simulators. Some are listed but not limited to (1) Safety, (2) Scarce resources, (3) Teaching/education, (4) Additional capabilities, (5) Flexibility and (6) Cost. Robot simulators are in use for all of these reasons. Virtual environments such as simulators will eliminate physical contact with humans and hence will increase the safety of work environment. Corporations with limited funding and resources may utilize simulators to accomplish their goals while saving manpower and money. A computer simulation is safer than working with a real robot. Robots are typically a scarce resource. Schools typically don t have a large number of robots, if any. Factories don t want the robots not performing useful work unless absolutely necessary. Robot simulators are useful in teaching robotics. A simulator gives a student hands-on experience, if only with a simulator. The simulator is more flexible. A user can quickly change the robot configuration, workcell, or even replace the robot with a different one altogether. In order to be useful, a robot simulator must create a model that accurately performs like the real robot. A powerful simulator is usually thought of as a combination of a CAD package with simulation capabilities. Computer Aided Design (CAD) techniques are used extensively by engineers in virtually all areas of engineering. Parts are designed interactively aided by the graphical display of both wireframe and more realistic shaded renderings. Once a part s dimensions have been specified to the CAD package, designers can view the part from any direction to examine how it will look and perform in relation to other parts. If changes are deemed necessary, the designer can easily make the changes and view the results graphically. However, a complex process of moving parts intended for operation in a complex environment can only be fully understood through the process of animated graphical simulation. A CAD package with simulation capabilities allows the designer to develop geometrical models of the process being designed, as well as the environment in which the process will be used, and then test the process in graphical animation much as the actual physical system would be run . By being able to operate the system of moving and stationary parts, the designer is able to see in simulation how the system will perform under a wide variety of conditions. If, for example, undesired collisions occur between parts of the system, design changes can be easily made without the expense or potential danger of testing the physical system.
Landcover test on Salt Lake test site illustrates potential issues with AWiFS/LISS-III for classification of certain land cover classes (evergreen, shrub/scrub, woody wetlands, emergent wetlands). Canopy and impervious graphs of product differences from source indicate slightly lower overall accuracies (shorter peaks, wider bases) for AWiFS/LISS-III, compared to L5/L7. Inspection of individual products from canopy and impervious estimate tests revealed issues with combining AWifs quadrants, and similar but less severe effects with combining multiple dates of L7 scan gap data.
As part of NASA's mission to explore habitable planets orbiting nearby stars, this article explores the detection and characterization capabilities of a 4 m space telescope plus 50 m starshade located at the Earth-Sun L2 point, known as the New Worlds Observer (NWO). Our calculations include the true spectral types and distribution of stars on the sky, an iterative target selection protocol designed to maximize efficiency based on prior detections, and realistic mission constraints. We conduct simulated observing runs for a wide range in exozodiacal background levels (epsilon = 1-100 times the local zodi brightness) and overall prevalence of Earth-like terrestrial planets (eta(sub solar halo))0.1-1). We find that even without any return visits, the NWO baseline architecture (IWA = 65 mas, limiting FPB = 4 x 10(exp -11) can achieve a 95% probability of detecting and spectrally characterizing at least one habitable Earth-like planet and an expectation value of approximately 3 planets found, within the mission lifetime and delta V budgets, even in the worst-case scenario (eta(sub solar halo) = 0.1 and = epsilon = 100 zodis for every target). This achievement requires about 1 yr of integration time spread over the 5 yr mission, leaving the remainder of the telescope time for UV-NIR general astrophysics. Cost and technical feasibility considerations point to a "sweet spot" in starshade design near a 50 m starshade effective diameter. with 12 or 16 petals, at a distance of 70,000-100,000 km from the telescope.
The National Aeronautics and Space Administration (NASA) is currently developing options for an Evolvable Mars Campaign (EMC) that expands human presence from Low Earth Orbit (LEO) into the solar system and to the surface of Mars. The Hybrid in-space transportation architecture is one option being investigated within the EMC. The architecture enables return of the entire in-space propulsion stage and habitat to cis-lunar space after a round trip to Mars. This concept of operations opens the door for a fully reusable Mars transportation system from cis-lunar space to a Mars parking orbit and back. This paper explores the reuse of in-space transportation systems, with a focus on the propulsion systems. It begins by examining why reusability should be pursued and defines reusability in space-flight context. A range of functions and enablers associated with preparing a system for reuse are identified and a vision for reusability is proposed that can be advanced and implemented as new capabilities are developed. Following this, past reusable spacecraft and servicing capabilities, as well as those currently in development are discussed. Using the Hybrid transportation architecture as an example, an assessment of the degree of reusability that can be incorporated into the architecture with current capabilities is provided and areas for development are identified that will enable greater levels of reuse in the future. Implications and implementation challenges specific to the architecture are also presented.
A major factor in long-term human exploration of the solar system is crop growth in microgravity. Space crops can provide fresh, nutritious food to supplement diets for astronauts. Important factors impacting space plant growth and consumption are water delivery to root zone in microgravity, sanitation methods for microbiological safety, plant responses to light quality/spectrum, and identifying optimal edible plants suitable for growth on the International Space Station (ISS). Astronauts growing their own food on the ISS provides necessary data for crop production for long duration deep space missions. The seed film project can be used in Advanced Plant Habitat and Veggies that are currently being utilized on the ISS.
Exploration of ballistic coefficient and entry flight path angle to determine loom requirements for weaving a dual-layer thermal protection material such as HEEET (Heatshield for Extreme Entry Environment Technology).
Exploration of ballistic coefficient and entry flight path angle to determine loom requirements for weaving a dual-layer thermal protection material such as HEEET
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It is possible that in the next twenty years NASA may fly crew quarters on twice as many spacecraft as it has in the past fifty years. In short, US experience with spacecraft crew quarters is limited and with few available standards to guide their design there is significant uncertainty facing spacecraft currently in development, several of which are also subject to substantial mass and volume challenges. Those spacecraft developments will face considerable pressure to minimize crew quarters size, including those intended for use on the lunar surface. Given that a crew quarters is the only space a crew member can call his or her own during missions that can last weeks to years in duration, providing an appropriate volume is especially important. This is even more critical when one considers the reality that all crew quarters flown to date have been smaller than minimum standards for US jail cells. This research will categorize functional capabilities of crew quarters and explore physical and virtual prototypes of small crew quarters that have attempted to include these capabilities. The Exploration Atmospheres Test at NASA Johnson Space Center represents the first opportunity to collect multi-day test data on crew quarters of this size in a gravitational environment. Intended to validate exploration prebreathe protocols, this test will house eight people inside a vacuum chamber that has been outfitted as a habitat prototype for twelve days. In addition to their other test activity, the crew will evaluate the acceptability of their crew quarters. This data will aid in establishing design guidelines for crew quarters in both short and long duration missions beyond low Earth orbit.
Urban Air Mobility (UAM) is an emerging aviation concept that could supplement today’s ground and air transportation systems. For UAM, it is generally assumed that the private sector will manage separation and not rely on the Federal Aviation Administration air traffic control system. To date, discussions of initial operations focus on using the visual abilities of the pilot to “see and avoid” (SAA) other aircraft. Decades of research on SAA has demonstrated that it is inadequate for reliable detection of aircraft that might pose a collision risk. The literature on multi-object tracking is also reviewed for findings on how well humans can visually track objects. The research shows that humans have limited resources for tracking and that this may be affected by object characteristics and cognitive skills. The conclusion is that SAA is a risky method for avoiding midair collisions. It is recommended that flight deck displays and automated collision avoidance systems be implemented for all UAM aircraft at the outset of their introduction.
Urban Air Mobility (UAM) is an emerging aviation concept that could supplement today’s ground and air transportation systems. For UAM, it is generally assumed that the private sector will manage separation and not rely on the Federal Aviation Administration air traffic control system. To date, discussions of initial operations focus on using the visual abilities of the pilot to “see and avoid” (SAA) other aircraft. Decades of research on SAA has demonstrated that it is inadequate for reliable detection of aircraft that might pose a collision risk. The literature on multi-object tracking is also reviewed for findings on how well humans can visually track objects. The research shows that humans have limited resources for tracking and that this may be affected by object characteristics and cognitive skills. The conclusion is that SAA is a risky method for avoiding midair collisions. It is recommended that flight deck displays and automated collision avoidance systems be implemented for all UAM aircraft at the outset of their introduction.
Urban Air Mobility (UAM) is an emerging aviation concept that could supplement today’s ground and air transportation systems. For UAM, it is generally assumed that the private sector will manage separation and not rely on the Federal Aviation Administration air traffic control system. To date, discussions of initial operations focus on using the visual abilities of the pilot to “see and avoid” (SAA) other aircraft. Decades of research on SAA has demonstrated that it is inadequate for reliable detection of aircraft that might pose a collision risk. The literature on multi-object tracking is also reviewed for findings on how well humans can visually track objects. The research shows that humans have limited resources for tracking and that this may be affected by object characteristics and cognitive skills. The conclusion is that SAA is a risky method for avoiding midair collisions. It is recommended that flight deck displays and automated collision avoidance systems be implemented for all UAM aircraft at the outset of their introduction.
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