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At least 55 records · Page 3

Tropical precipitation rates during SOP-1, FGGE, estimated from heat and moisture budgets

Using the NASA Goddard Laboratory analyses collected during the first FGGE Special Observing Period, global estimates of precipitation rates were derived for the domain between 30-deg N to 30-deg S, and the results were compared to OLR patterns and to each other to evaluate their consistency and reliability. Regional averages are presented to examine the variability of rainfall rates among selected regions of the Southern Hemisphere tropics. Finally, precipitable water was computed and compared to results derived from SMMR estimates and to the precipitation patterns. Results show that the heat and moisture budget estimates of precipitation compare favorably. Vertical profiles reveal that maximum convective heating occurs in the middle troposphere. The profile of the South Pacific convergence zone region compares best with profiles obtained over the western North Pacific.

Pedigo, Catherine B.↗

Observational and modeling study of the maintenance of short-term subtropical wind maxima in the Southern Hemisphere during SOP-1, FGGE

The findings of the earlier observational work of Hurrel and Vincent (1990) are substantiated using a recent version of the Goddard Laboratory for Atmospheres fourth-order general circulation model. The observed heating and subtropical zonal flow structure over the South Pacific is reproduced by a control integration with full model physics, for the period of 6-20 January 1979. A subtropical westerly maximum observed over the central South Pacific is found to be highly correlated to enhancements of poleward-moving divergent outflow from tropical heat sources. The divergent meridional flow accounted for much of the total ageostrophic meridional wind in the Southern Hemisphere subtropics, and, consequently, resulted in strong Coriolis accelerations in the entrance regions westerly maxima.

Hurrell, James W.↗

Coastal Observations of Weather Features in Senegal during the AMMA SOP-3 Period

During 15 August through 30 September 2006, ground and aircraft measurements were obtained from a multi-national group of students and scientists in Senegal. Key measurements were aimed at investigating and understanding precipitation processes, thermodynamic and dynamic environmental conditions, cloud, aerosol and microphysical processes and spaceborne sensors (TRMM, CloudSat/Calipso) validation. Ground and aircraft instruments include: ground based polarimetric radar, disdrometer measurements, a course and a high-density rain gauge network, surface chemical measurements, a 10 m flux tower, broadband IR, solar and microwave measurements, rawinsonde and radiosonde measurements, FA-20 dropsonde, in situ microphysics and cloud radar measurements. Highlights during SOP3 include ground and aircraft measurements of squall lines, African Easterly Waves (AEWs), Saharan Air Layer advances into Senegal, and aircraft measurements of AEWs -- including the perturbation that became Hurricane Isaac.

Jenkins, G.↗

Comments on "Atlantic Tropical Cyclogenetic Processes during SOP-3 NAMMA in the GEOS-5 Global Data Assimilation and Forecast System"

Considerable attention has been given to the potential negative impacts of the Saharan air layer (SAL) in recent years. Researchers recently raised questions about the negative impacts of Dunion and Velden and other studies in terms of storms that reached at least tropical storm strength and suggested that the SAL was an intrinsic part of the tropical cyclone environment for both storms that weaken after formation and those that intensify. Braun also suggested that several incorrect assumptions underlie many of the studies on the negative impacts of the SAL, including assumptions that most low-to-midlevel dry tropical air is SAL air, that the SAL is dry throughout its depth, and that the proximity of the SAL to storms struggling to intensify implies some role in that struggle. The recent paper by Reale et al.(RL1) is an example of the problems inherent in some of these assumptions. In their paper, RL1 analyze a simulation from the Global Earth Observing System (GEOS-5) global model and describe an extensive tongue of warm, dry air that stretches southward from at least 30 deg N (the northern limit of their plots) and wraps into a low pressure system during the period 26-29 August 2006, suppressing convection and possibly development of the African easterly wave associated with that low pressure system. They attributed the warm, dry tongue to the SAL (i.e., heating of the air mass during passage over the Sahara and radiative warming of the dust layer). Whether it was their intention, the implication is that this entire feature is due solely to the SAL and not to other possible sources of dry air or warmth. In addition, they suggested that a cool tongue of air in the boundary layer located directly beneath the elevated warm, dry tongue (forming a thermal dipole) was possibly the result of reduced solar radiation caused by an overlying dust layer. They stated that "the cool anomaly in the lower levels does not have any plausible explanation relying only on transport." In this comment, evidence from satellite and global meteorological analyses is presented that casts considerable doubt upon RL1 s interpretation of the GEOS-5 forecasts and their conclusion that the results implied a negative role of the SAL. We show that the major portion of the warm, dry air aloft was located in a nearly dust-free slot between two Saharan dust outbreaks, had a significant source from the midlatitudes (>30 N), and was likely driven by strong subsidence warming and drying. In addition, when wind fields are examined in a reference frame moving with the wave, National Centers for Environmental Prediction (NCEP) global meteorological analyses suggest that the cool tongue in the boundary layer can be readily explained by transport of cooler air from higher latitudes. At the very least, it offers a plausible alternative explanation for the cool tongue that does not rely on radiative impacts of the dust.

Braun, Scott A.↗

Energetics analysis of the global circulation during the special observation periods of FGGE

A global energetics analysis is presented for the FGGE SOP-1 and SOP-2 with ECMWF and GFDL data sets. Both global integral properties and spectral characteristics are examined. There is a large discrepancy between the present FGGE analysis and previously available estimates concerning the level of available potential energy and kinetic energy. This discrepancy is attributable to an earlier restriction of data coverage. There is a significant seasonal difference in the energy reservoir of the Northern Hemisphere, whereas the diference is minor in the Southern Hemisphere. This leads to a seasonal contrast of the globally integrated energy budget which comes mostly from the Northern Hemisphere. Both the global energy level and the intensity of the general circulation are more pronounced during SOP-1 than during SOP-2. One major focus of attention in this study is an energetics comparison of ECMWF and GFDL data sets. There is a twofold difference in the intensity of the general circulation as measured with these two data sets. The energetics diagnosis indicates that the operational modes of the general circulation as described by these two data sets also differ considerably. The contrasts between SOP-1 and SOP-2 and between the ECMWF and GFDL versions of the data sets are apparent in the pattern of energy flow in the wavenumber domain.

Kung, E. C.↗

Chemical Hygiene Program

The Chemical Management Team is responsible for ensuring compliance with the OSHA Laboratory Standard. The program at Lewis Research Center (LeRC) evolved over many years to include training, developing Standard Operating Procedures (SOPS) for each laboratory process, coordinating with other safety and health organizations and teams at the Center, and issuing an SOP binder. The Chemical Hygiene Policy was first established for the Center. The Chemical Hygiene Plan was established and reviewed by technical, laboratory and management for viability and applicability to the Center. A risk assessment was conducted for each laboratory. The laboratories were prioritized by order of risk, higher risk taking priority. A Chemical Management Team staff member interviewed the lead researcher for each laboratory process to gather the information needed to develop the SOP for the process. A binder containing the Chemical Hygiene Plan, the SOP, a map of the laboratory identifying the personal protective equipment and best egress, and glove guides, as well as other guides for safety and health. Each laboratory process has been captured in the form of an SOP. The chemicals used in the procedure have been identified and the information is used to reduce the number of chemicals in the lab. The Chemical Hygiene Plan binder is used as a training tool for new employees. LeRC is in compliance with the OSHA Standard. The program was designed to comply with the OSHA standard. In the process, we have been able to assess the usage of chemicals in the laboratories, as well as reduce or relocate the chemicals being stored in the laboratory. Our researchers are trained on the hazards of the materials they work with and have a better understanding of the hazards of the process and what is needed to prevent any incident. From the SOP process, we have been able to reduce our chemical inventory, determine and implement better hygiene procedures and equipment in the laboratories, and provide specific training to our employees. As a result of this program, we are adding labeling to the laboratories for emergency responders and initiating a certified chemical user program.

Mayor, Antoinette C.↗

Robust surfactant-assisted one-pot sample preparation for label-free single-cell and nanoscale proteomics

With advanced mass spectrometry (MS)-based proteomics, genome-scale proteome coverage can be achieved from bulk cells. However, such bulk measurement obscures cell to cell heterogeneity, precluding proteome profiling of single cells and small numbers of cells of interest. To address this issue, in recent 5 years there are a surge of small sample preparation methods developed for robust effective collection and processing of single cells and small numbers of cells for in-depth MS-based proteome profiling. Based on their broad accessibility, they can be categorized into two types: specific device- and standard PCR tube- or multi-well plate-based methods. Herein we describe the detailed protocol of our recently developed, easily adoptable, Surfactant-assisted One-Pot (SOP) sample preparation coupled with MS method termed SOP-MS for label-free single-cell and nanoscale proteomics. SOP-MS capitalizes on the combination of a MS-compatible surfactant, DDM (n-Dodecyl-ß-D-maltoside), and standard low-bind PCR tube or multi-well plate for ‘all-in-one’ one-pot sample preparation without sample transfer. With its robust and convenient features, SOP-MS can be readily implemented in any MS laboratory for single-cell and nanoscale proteomics. With further improvements in MS detection sensitivity and sample throughput, we believe that SOP-MS could open an avenue for single-cell proteomics with broad applicability in the biological and biomedical research.

Single-cell proteomics, nanoscale proteomics, SOP-↗

Facile One-Pot Nanoproteomics for Label-Free Proteome Profiling of 50–1000 Mammalian Cells

Recent advances in sample preparation enable label-free MS-based proteome profiling of small numbers of mammalian cells. However, specific devices are often required to downscale sample processing volume from the standard 50-200 µL to sub-µL for effective nanoproteomics, which greatly impedes the implementation of current nanoproteomics methods by broad proteomics research community. Here we report a facile one-pot nanoproteomics method termed SOPs-MS (Surfactant-assisted One-Pot sample processing at the standard volume coupled with MS) for convenient proteome profiling of 50-1000 mammalian cells. Building upon our recent development of SOP-MS for label-free single-cell proteomics at low µL volume (Commun Bio 2021, 4, 265), we have systematically evaluated its processing volume at 10-200 µL using 100 human cells for robust reproducible nanoproteomic analysis. The processing volume of 50 µL which is in the range of volume for standard proteomics sample preparation, has been selected for easy sample handling with benchtop micropipette. Using the commonly accessible LC-MS platform, SOPs-MS allows for reliable label-free quantification of ~1200-2700 protein groups from 50-1000 MCF10A cells. When applied to small subpopulations of mouse colon crypt cells, SOPs-MS can reveal distinct protein signatures between any two subpopulation cells with identification of ~1500-2500 protein groups for each subpopulation. SOPs-MS may pave the way for routine deep proteome profiling of small numbers of cells as well as low-input samples.

59 BASIC BIOLOGICAL SCIENCES↗

Localized spin-orbit polaron in magnetic Weyl semimetal Co 3 Sn 2 S 2

The kagome lattice Co 3 Sn 2 S 2 exhibits the quintessential topological phenomena of a magnetic Weyl semimetal such as the chiral anomaly and Fermi-arc surface states. Probing its magnetic properties is crucial for understanding this correlated topological state. Here, using spin-polarized scanning tunneling microscopy/spectroscopy (STM/S) and non-contact atomic force microscopy (nc-AFM) combined with first-principle calculations, we report the discovery of localized spin-orbit polarons (SOPs) with three-fold rotation symmetry nucleated around single S-vacancies in Co 3 Sn 2 S 2. The SOPs carry a magnetic moment and a large diamagnetic orbital magnetization of a possible topological origin associated relating to the diamagnetic circulating current around the S-vacancy. Appreciable magneto-elastic coupling of the SOP is detected by nc-AFM and STM. Our findings suggest that the SOPs can enhance magnetism and more robust time-reversal-symmetry-breaking topological phenomena. Controlled engineering of the SOPs may pave the way toward practical applications in functional quantum devices.

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗

Senseless acts as a binary switch during sensory organ precursor selection

During sensory organ precursor (SOP) specification, a single cell is selected from a proneural cluster of cells. Here, we present evidence that Senseless (Sens), a zinc-finger transcription factor, plays an important role in this process. We show that Sens is directly activated by proneural proteins in the presumptive SOPs and a few cells surrounding the SOP in most tissues. In the cells that express low levels of Sens, it acts in a DNA-binding-dependent manner to repress transcription of proneural genes. In the presumptive SOPs that express high levels of Sens, it acts as a transcriptional activator and synergizes with proneural proteins. We therefore propose that Sens acts as a binary switch that is fundamental to SOP selection.

NASA Program Fundamental Space Biology↗

Standard Operating Procedure for Optimal Deployment of Meteorological Instrumentation Within the Solar Radiation Research Laboratory: 2024 Edition

The objective of the National Renewable Energy Laboratory's (NREL's) Solar Radiation Research Laboratory (SRRL) is to collect and use high-quality solar radiation data sets for research leading to the widespread adoption of solar technologies. To appropriately populate and track the diverse array of instruments at the NREL-SRRL, NREL has established a Standard Operating Procedure (SOP) for optimal instrument deployment within the SRRL for both the Baseline Measurement System (BMS) and the Research Measurement System (RMS). Using best practices methodologies, the NREL-SRRL maintains a varied and extensive array of solar monitoring equipment to test, evaluate, and characterize the solar sensors used by federal and international agencies as well as the solar industry to determine the solar resource. The SOP provides the industry with guidance for solar resource assessment and is used for procedures in the long-term continuous monitoring of legacy instruments alongside state-of-the-art instruments. Based on the SOP, instruments are annually evaluated for continued deployment. Instruments that do not meet the SOP criteria are decommissioned, and new instruments that meet the criteria are deployed. Streamlining and optimizing the use of this facility ensures that the lab continues to be a world-leading solar calibration and measurement facility. This 2024 edition includes updates to the appendices to reflect the instrument changes from one year to another.

14 SOLAR ENERGY↗

Standard Operating Procedure for Optimal Deployment of Meteorological Instrumentation Within the Solar Radiation Research Laboratory: 2025 Edition

The objective of the National Renewable Energy Laboratory's (NREL's) Solar Radiation Research Laboratory (SRRL) is to collect and use high-quality solar radiation data sets for research leading to the widespread adoption of solar technologies. To appropriately populate and track the diverse array of instruments at the NREL-SRRL, NREL has established a Standard Operating Procedure (SOP) for optimal instrument deployment within the SRRL for both the Baseline Measurement System (BMS) and the Research Measurement System (RMS). Using best practices methodologies, the NREL-SRRL maintains a varied and extensive array of solar monitoring equipment to test, evaluate, and characterize the solar sensors used by federal and international agencies as well as the solar industry to determine the solar resource. The SOP provides the industry with guidance for solar resource assessment and is used for procedures in the long-term continuous monitoring of legacy instruments alongside state-of-the-art instruments. Based on the SOP, instruments are annually evaluated for continued deployment. Instruments that do not meet the SOP criteria are decommissioned, and new instruments that meet the criteria are deployed. Streamlining and optimizing the use of this facility ensures that the lab continues to be a world-leading solar calibration and measurement facility. This 2025 edition includes updates to the appendices to describe the current instrumentation of the NREL-SRRL.

14 SOLAR ENERGY↗

Influence of liquid conductance on the temporal evolution of self-organization patterns in atmospheric pressure DC glow discharges

Abstract Self-Organized Patterns (SOPs) at plasma-liquid interface in atmospheric pressure plasma discharges refer to the formation of intricate and puzzling structures due to the interplay of electrodynamic and hydrodynamic processes. Studies conducted to date have shown that this phenomenon results in the formation of distinctive patterns such as circular ring, star, gear, dots, spikes, etc., and primarily depends on working gas, electrolyte type, gap distance, current, conductivity, etc. However, an adequate understanding of how these patterns change from one type to another is still not available. This study aims to elucidate the influence of initial liquid conductance ( σ i ) on the temporal evolution of SOPs in liquid-anode discharges. The discharge was generated in a pin-to-liquid anode configuration at a constant helium (He) flow rate of 500 sccm and DC applied voltage of 6 kV at a gap distance of 12 mm. Through the gradual increment of σ i from 1.8 μ S to 4820 μ S, we observe that the trend in the evolution of SOPS takes place as solid discs, spikes, dots, rings, double rings, and stars. The continuous formation of reactive species onto the liquid anode in all conductive solutions results in a decrease in pH, an increase in bulk liquid temperature, and an increase in total dissolved solutes, and these have been confirmed through experimental measurements. Observations using optical emission spectroscopy show that the electrons at the plasma-liquid interface participate in the reduction of cations followed by their excitation & ionization due to which electron density as well as emissions from excited species (mainly hydroxyl radicals & excited nitrogen) decrease with time. Our investigation provides experimental evidence on the presence of cations at the plasma-liquid interface required for SOP formation.

Physics↗

Reducing Spontaneous Orientational Polarization via Semiconductor Dilution Improves OLED Efficiency and Lifetime

Spontaneous orientational polarization (SOP) in the electron-transport layer (ETL) of organic light-emitting diodes (OLEDs) is increasingly recognized as a key factor influencing their performance. Here, we show that SOP is dramatically reduced in the common electron-transport material 2,2',2"-(1,3,5-benzinetriyl)-tris(1-phenyl-1-H-benzimidazole) by coevaporating it together with medium-density polyethylene. Eliminating SOP from the ETL of blue fluorescent OLEDs in this manner reduces their operating voltage by 0.5 V, increases their external quantum efficiency (EQE) by 30%, and leads to a threefold increase in device lifetime. We show that the EQE and lifetime improvements both originate from reduced exciton-polaron annihilation in the emissive layer, and that this leads to a functional relationship between the two quantities that can be used to quantify the rate of annihilation-induced degradation in the device. Furthermore, these results highlight a substantial opportunity to improve OLED performance by controlling SOP through semiconductor dilution and suggest that this capability can be used to systematically isolate and understand exciton-polaron degradation in the pursuit of stable blue OLEDs.

36 MATERIALS SCIENCE↗

On testing stuck-open faults in CMOS combinational circuits

Recently it has been found that a class of failure related to a particular technology (CMOS) cannot be modelled as the conventional stuck-at fault model. These failures change the combinational behavior of CMOS logic gates into a sequential one. Such a failure is modelled as a fault, called the Stuck-Open fault (SOP). The object of this paper is to develop a procedure to detect single SOPs in combinational circuits. It is shown, that in general, tests generated for stuck-at faults when applied in a particular sequence will detect all single SOP faults. In case of single redundancy in the network, the SOP fault on the redundant line cannot be detected. When there is reconvergent fan-out in the network, there is a one-one correspondence between the conditions for stuck-at fault and stuck-open fault detectability.

Chandramouli, R.↗

Surfactant-assisted one-pot sample preparation for label-free single-cell proteomics

Large numbers of cells are generally required for quantitative global proteome profiling due to surface adsorption losses associated with sample processing. Such bulk measurement obscures important cell-to-cell variability (cell heterogeneity) and makes proteomic profiling impossible for rare cell populations (e.g., circulating tumor cells (CTCs)). Here we report a surfactant-assisted one-pot sample preparation coupled with mass spectrometry (MS) method termed SOP-MS for label-free global single-cell proteomics. SOP-MS capitalizes on the combination of a MS-compatible nonionic surfactant, n-Dodecyl-β-D-maltoside, and hydrophobic surface-based low-bind tubes or multi-well plates for ‘all-in-one’ one-pot sample preparation. This ‘all-in-one’ method including elimination of all sample transfer steps maximally reduces surface adsorption losses for effective processing of single cells, thus improving detection sensitivity for single-cell proteomics. This method allows convenient label-free quantification of hundreds of proteins from single human cells and ~1200 proteins from small tissue sections (close to ~20 cells). When applied to a patient CTC-derived xenograft (PCDX) model at the single-cell resolution, SOP-MS can reveal distinct protein signatures between primary tumor cells and early metastatic lung cells, which are related to the selection pressure of anti-tumor immunity during breast cancer metastasis. The approach paves the way for routine, precise, quantitative single-cell proteomics.

36 MATERIALS SCIENCE↗

Comparative energetics of the observed and simulated global circulation during the special observing periods of FGGE

Energetics of the observed and simulated global circulation are evaluated in the zonal spectral domain for the special observing periods of FGGE. The study utilizes GLA analyses of FGGE observational data and parallel simulation experiments. There are noticeable differences in energy transformations between the observation and simulation during SOP-1. These include the baroclinic conversion C(n) by the zonal mean motion and short-wave disturbances, and the nonlinear wave-wave interaction L(n) at the long and short waves. The energy transformations of the short-wave disturbances are much more intense in the simulated circulation than in the observation. However, good agreement is noted in the conversion and dissipation of kinetic energy in the large- and cyclone-wave range n = 1-10. Spectral distributions of global energy transformations at the long- and cyclone-wave range indicate that the SOP-2 simulation agrees more closely with the observed fields than the SOP-1 simulation. Other pertinent points of energetics diagnosis are also included in the discussion.

Kung, E. C.↗