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NASA NTRS1948

NACA: University Conference on Aerodynamics

This document contains reproductions of the technical papers presented at the NACA - University Conference on Aerodynamics held at the Langley Aeronautical Laboratory on June 21, 22, and 23, 1948. The conference was held in recognition of the difficulties, imposed by security restrictions, in keeping abreast of the rapid advances in aerodynamics. The papers were prepared to review the status of a number of fields of interest, to summarize the more important wartime advances that are no longer classified, and to orient reference material for further study. The papers in this document are in the same form in which they were presented at the conference so that distribution of them might be prompt. The original presentation and this record are considered as complementary to, rather than as substitutes for, the Committee?s system of complete and formal reports.

Source record↗
NASA NTRS1992

Integrated software package STAMP for minor planets

The integrated software package STAMP allowed for rapid and exact reproduction of the tables of the year-book 'Ephemerides of Minor Planets.' Additionally, STAMP solved the typical problems connected with the use of the year-book. STAMP is described. The year-book 'Ephemerides of Minor Planets' (EMP) is a publication used in many astronomical institutions around the world. It contains all the necessary information on the orbits of the numbered minor planets. Also, the astronomical coordinates are provided for each planet during its suitable observation period.

Kochetova, O. M.↗
NASA NTRS2003

Non-Genomic Origins of Proteins and Metabolism

It is proposed that evolution of inanimate matter to cells endowed with a nucleic acid- based coding of genetic information was preceded by an evolutionary phase, in which peptides not coded by nucleic acids were able to self-organize into networks capable of evolution towards increasing metabolic complexity. Recent findings that truly different, simple peptides (Keefe and Szostak, 2001) can perform the same function (such as ATP binding) provide experimental support for this mechanism of early protobiological evolution. The central concept underlying this mechanism is that the reproduction of cellular functions alone was sufficient for self-maintenance of protocells, and that self- replication of macromolecules was not required at this stage of evolution. The precise transfer of information between successive generations of the earliest protocells was unnecessary and, possibly, undesirable. The key requirement in the initial stage of protocellular evolution was an ability to rapidly explore a large number of protein sequences in order to discover a set of molecules capable of supporting self- maintenance and growth of protocells. Undoubtedly, the essential protocellular functions were carried out by molecules not nearly as efficient or as specific as contemporary proteins. Many, potentially unrelated sequences could have performed each of these functions at an evolutionarily acceptable level. As evolution progressed, however proteins must have performed their functions with increasing efficiency and specificity. This, in turn, put additional constraints on protein sequences and the fraction of proteins capable of performing their functions at the required level decreased. At some point, the likelihood of generating a sufficiently efficient set of proteins through a non-coded synthesis was so small that further evolution was not possible without storing information about the sequences of these proteins. Beyond this point, further evolution required coupling between proteins and informational polymers that is characteristic to all known forms of life. The emergence of such coupling must be postulated in any scenario of the origin of life, no matter whether it starts with RNA or proteins. To examine the evolutionary potential of non-genomic systems, a simple, computationally tractable model, which is still capable of capturing the essential features of the real system, has been studied computationally. Both constructive and destructive processes have been introduced into the model in a stochastic manner. Instead of assuming random reaction sets, only a suite of protobiologically plausible reactions has been considered. Peptides have been explicitly considered as protoenzymes and their catalytic efficiencies have been assigned on the basis of biochemical principles and experimental estimates. Simulations have been carried out using a novel approach (The Next Reaction Method) that is appropriate even for very low concentrations of reactants. Studies have focused on global autocatalytic processes and their diversity.

Pohorille, Andrew↗
NASA NTRS2014

The Affect of the Space Environment on the Survival of Halorubrum Chaoviator and Synechococcus (Nageli): Data from the Space Experiment OSMO on EXPOSE-R

We have shown using ESA's Biopan facility flown in Earth orbit that when exposed to the space environment for 2 weeks the survival rate of Synechococcus (Nageli), a halophilic cyanobacterium isolated from the evaporitic gypsum-halite crusts that form along the marine intertidal, and Halorubrum chaoviator a member of the Halobacteriaceae isolated from an evaporitic NaCl crystal obtained from a salt evaporation pond, were higher than all other test organisms except Bacillus spores. These results led to the EXPOSE-R mission to extend and refine these experiments as part of the experimental package for the external platform space exposure facility on the ISS. The experiment was flown in February 2009 and the organisms were exposed to low-Earth orbit for nearly 2 years. Samples were either exposed to solar ultraviolet (UV)-radiation (lambda is greater than 110 nm or lambda is greater than 200 nm, cosmic radiation (dosage range 225-320 mGy), or kept in darkness shielded from solar UV-radiation. Half of each of the UV-radiation exposed samples and dark samples were exposed to space vacuum and half kept at 105 pascals in argon. Duplicate samples were kept in the laboratory to serve as unexposed controls. Ground simulation control experiments were also performed. After retrieval, organism viability was tested using Molecular Probes Live-Dead Bac-Lite stain and by their reproduction capability. Samples kept in the dark, but exposed to space vacuum had a 90 +/- 5% survival rate compared to the ground controls. Samples exposed to full UV-radiation for over a year were bleached and although results from Molecular Probes Live-Dead stain suggested approximately 10% survival, the data indicate that no survival was detected using cell growth and division using the most probable number method. Those samples exposed to attenuated UV-radiation exhibited limited survival. Results from of this study are relevant to understanding adaptation and evolution of life, the future of life beyond earth, the potential for interplanetary transfer of viable microbes via meteorites and dust particles as well as spacecraft, and the physiology of halophiles.

space flight↗
NASA NTRS1996

Radiation-induced chromosomal instability in human mammary epithelial cells

Karyotypes of human cells surviving X- and alpha-irradiation have been studied. Human mammary epithelial cells of the immortal, non-tumorigenic cell line H184B5 F5-1 M/10 were irradiated and surviving clones isolated and expanded in culture. Cytogenetic analysis was performed using dedicated software with an image analyzer. We have found that both high- and low-LET radiation induced chromosomal instability in long-term cultures, but with different characteristics. Complex chromosomal rearrangements were observed after X-rays, while chromosome loss predominated after alpha-particles. Deletions were observed in both cases. In clones derived from cells exposed to alpha-particles, some cells showed extensive chromosome breaking and double minutes. Genomic instability was correlated to delayed reproductive death and neoplastic transformation. These results indicate that chromosomal instability is a radiation-quality-dependent effect which could determine late genetic effects, and should therefore be carefully considered in the evaluation of risk for space missions.

NASA Center JSC↗
NASA NTRS2000

Experience with a Genetic Algorithm Implemented on a Multiprocessor Computer

Numerical experiments were conducted to find out the extent to which a Genetic Algorithm (GA) may benefit from a multiprocessor implementation, considering, on one hand, that analyses of individual designs in a population are independent of each other so that they may be executed concurrently on separate processors, and, on the other hand, that there are some operations in a GA that cannot be so distributed. The algorithm experimented with was based on a gaussian distribution rather than bit exchange in the GA reproductive mechanism, and the test case was a hub frame structure of up to 1080 design variables. The experimentation engaging up to 128 processors confirmed expectations of radical elapsed time reductions comparing to a conventional single processor implementation. It also demonstrated that the time spent in the non-distributable parts of the algorithm and the attendant cross-processor communication may have a very detrimental effect on the efficient utilization of the multiprocessor machine and on the number of processors that can be used effectively in a concurrent manner. Three techniques were devised and tested to mitigate that effect, resulting in efficiency increasing to exceed 99 percent.

Plassman, Gerald E.↗
NASA NTRS2000

Experience with a Genetic Algorithm Implemented on a Multiprocessor Computer

Numerical experiments were conducted to find out the extent to which a Genetic Algorithm (GA) may benefit from a multiprocessor implementation, considering, on one hand, that analyses of individual designs in a population are independent of each other so that they may be executed concurrently on separate processors, and, on the other hand, that there are some operations in a GA that cannot be so distributed. The algorithm experimented with was based on a gaussian distribution rather than bit exchange in the GA reproductive mechanism, and the test case was a hub frame structure of up to 1080 design variables. The experimentation engaging up to 128 processors confirmed expectations of radical elapsed time reductions comparing to a conventional single processor implementation. It also demonstrated that the time spent in the non-distributable parts of the algorithm and the attendant cross-processor communication may have a very detrimental effect on the efficient utilization of the multiprocessor machine and on the number of processors that can be used effectively in a concurrent manner. Three techniques were devised and tested to mitigate that effect, resulting in efficiency increasing to exceed 99 percent.

Plassman, Gerald E.↗
NASA NTRS1984

Hypergravity effects on litter size, nursing activity, prolactin, TSH, T3, and T4 in the rat

In a recent study of the effects of hypergravity (HG) on the reproductive system of the rat, it was found that the estrous cycle is perturbed in a pseudopregnancy-like pattern upon first exposure of animals to HG. This can be prevented by previous exposure to HG or by administration of bromergo cryptin. Adapted rats can mate and deliver in HG. However, little is known of the condition of the individual pregnant rat, fetuses, and newborn pups in this environment. There are also questions regarding the effects of HG on the number of fetuses, the mortality rate in different HG levels, and the effects on production and secretion of PRL and TSH. The present investigation is concerned with such questions, taking into account possible approaches to avoid or minimize the lethal effects of HG. The obtained results suggest that peripartum plasma PRL levels and thyroid hormone levels are critical for the survival of the litter.

Megory, E.↗
NASA NTRS1990

Nocturnal illumination maintains reproductive function and simulates the period-lengthening effect of constant light in the mature male Djungarian hamster (Phodopus sungorus)

Mature male Djungarian hamsters (Phodopus sungorus) were placed in individual light-tight, sound attenuated chambers and exposed to one of four lighting conditions for a duration of approximately seven weeks. The four lighting conditions were: constant light (LL); constant dark (DD); feedback lighting (LDFB; a condition that illuminates the cage in response to locomotor activity); or a feedback lighting neighbor control (LDFB NC; the animal receives the same light pattern as a paired animal in feedback lighting, but has no control over it). Exposure of hamsters to LL or LDFB produced significantly and similarly longer free-running periods of the locomotor activity rhythm than exposure of animals to DD. Hamsters exposed to LDFB NC did not free-run or entrain, but rather displayed "relative coordination". The paired testes and sex accessory glands weights suggest that in the Djungarian hamster, LL and LDFB exposed animals maintained reproductive function, whereas DD exposed animals did not. Animals exposed to LDFB NC had intermediate paired testes weights. Since several previous studies have demonstrated that short pulses of light, which are coincident with the subjective night, are photostimulatory, it is not surprising that LDFB maintained reproductive function in the mature Djungarian hamster. Feedback lighting, however, has been shown to be an insufficient stimulus to maintain reproductive function of mature male and female Syrian hamsters, and to the reproductive maturation of immature Djungarian hamsters. The results suggest that there may be slight, but significant differences in the way these two species interpret photoperiod, as well as a developmental change in the photoperiodic response of Djungarian hamsters.

NASA Program Space Biology↗
NASA NTRS2004

The Hindlimb Unloading Rat Model: Literature Overview, Comparison with Spaceflight Data, and Technique Update

The hindlimb unloading (HU) rodent model is used extensively to study the response of many physiological systems to certain aspects of spaceflight, as well as to disuse and recovery from disuse for Earth benefits. This chapter describes the evolution of HU, and is divided into three sections. The first section examines the characteristics of 1063 articles using or reviewing the HU model, published between 1976 and April 1, 2004. The characteristics include number of publications, journals, countries, major physiological systems, method modifications, species, gender, genetic strains and ages of rodents, experiment duration, and countermeasures. The second section provides a comparison of results between space flown and Hu animals from the 14-day Cosmos 2044 mission. The final section describes modifications to HU required by different experimental paradigms and a method to protect the tail harness for long duration studies. HU in rodents has enabled improved understanding of the responses of the musculoskeletal, cardiovascular, immune, renal, neural, metabolic, and reproductive systems to unloading and/or to reloading on Earth with implications for both long-duration human spaceflight and disuse on Earth.

Morey-Holton, Emily↗
NASA NTRS2005

The hindlimb unloading rat model: literature overview, technique update and comparison with space flight data

The hindlimb unloading rodent model is used extensively to study the response of many physiological systems to certain aspects of space flight, as well as to disuse and recovery from disuse for Earth benefits. This chapter describes the evolution of hindlimb unloading, and is divided into three sections. The first section examines the characteristics of 1064 articles using or reviewing the hindlimb unloading model, published between 1976 and April 1, 2004. The characteristics include number of publications, journals, countries, major physiological systems, method modifications, species, gender, genetic strains and ages of rodents, experiment duration, and countermeasures. The second section provides a comparison of results between space flown and hindlimb unloading animals from the 14-day Cosmos 2044 mission. The final section describes modifications to hindlimb unloading required by different experimental paradigms and a method to protect the tail harness for long duration studies. Hindlimb unloading in rodents has enabled improved understanding of the responses of the musculoskeletal, cardiovascular, immune, renal, neural, metabolic, and reproductive systems to unloading and/or to reloading on Earth with implications for both long-duration human space flight and disuse on Earth.

Flight Experiment↗
NASA NTRS1996

Adaptation of Motility Analysis Apparatus for Space Science and Microgravity Ground-Based Experiments

Previous space flight studies have described unfavorable effects of microgravity on testicular morphology and spermatogenesis (Cosmos 1887 Biosputnik flight, 9/29/87 - 10/12/87). The flight animals demonstrated small reductions in testicular and epididymal size, the phenomenon explained as resulting water loss. Yet, light microscopic histological preparations revealed few spermatozoa in the rete testis of the flight males compared to control animals. The cause for this finding was subjectively assessed to be due to "the anatomical dislocation of the organs... and a disturbance in testicular blood supply". Unfortunately, the reported effects of microgravity on the reproductive processes (particularly within males) are few and divergent. If habitation in space is a futuristic goal, more objective testing (of male and female gametes) in a microgravity environment will provide insight to the developmental potential of these reproductive cells. As part of the Marshall Space Flight Centers' Summer Faculty Fellowship Program within the Biophysics Branch, a key component of the research investigation was to develop a test to evaluate individual cell motility and orientation in varying gravitational environments, using computerized assessment of sperm cell concentration, morphology and motility to provide objective, quantitative experimental control. In previous work performed jointly by the author and a NASA colleague, it has been shown that macroscopic motile aggregates of spermatozoa were not altered by the absence of microgravity. Variations in the number of normal versus abnormal sperm due to microgravity influences have yet to be established. It is therefore of interest to monitor the cytoskeletal matrix (microtubulin) of these organisms as a possible indicator of cell viability and/or function.

Johnson, Jacqueline U.↗
NASA NTRS1988

The effects of feedback lighting on the circadian rhythm of locomotor activity and the reproductive maturation of the male Djungarian hamster (Phodopus sungorus)

The non-parametric model of entrainment suggests that brief pulses of light, delivered between dusk and dawn can simulate the phasing effects of full photoperiods or even constant light (LL). Feedback lighting (LDFB) is a lighting condition where individual animals, otherwise in constant darkness (DD), are exposed to light in response to a monitored behavior. The specific purpose of this type of illumination is to expose the circadian cycle to light only during the subjective night. LDFB has been used to support this hypothesis in several species of nocturnal rodents and one species of diurnal primate by producing similar free-running periods in LDFB as in LL. This lighting condition has also been used to test the hypothesis that exposing the subjective night to even short duration light pulses will maintain reproductive function in long day breeders. In the Syrian hamster (Mesocricetus auratus), however, LDFB is not as photostimulatory as LL despite extensive light exposure during the subjective night. In the experiments presented here, a group of immature male Djungarian hamsters (Phodopus sungorus) were placed in individual light-tight sound attenuated chambers where they had free access to food, water and an activity wheel. The animals were exposed to one of four lighting conditions [DD, LL, LDFB or a neighbor control of feedback lighting (LDFB NC)] for approximately 30 days shortly after weaning. LDFB NC is a lighting condition where a neighbor control hamster receives the identical lighting regime as a paired animal exposing itself to LDFB, yet the neighbor has no control over it. A fifth group was exposed to a light-dark cycle of 16 hours of light and 8 hours of dark (LD16:8). This group was housed in cages in a colony room and did not have access to a running wheel. The free-running periods of the locomotor activity rhythms for hamsters exposed to LDFB and LL were not similar, unlike the results for rats, Syrian hamsters, mice, monkeys and even mature Djungarian hamsters. Immature hamsters exposed to DD and LDFB NC developed more slowly than animals exposed to LL or LD16:8, while hamsters in LDFB developed at an intermediate rate. Thus, it appears that LDFB, although capable of inducing reproductive function in immature Djungarian hamsters, is not as photostimulatory as may have been expected from current photoperiodic models, despite substantial light exposure during the subjective night. Furthermore, this data may suggest that the circadian system of 18-48 day old Djungarian hamsters are still undergoing organizational maturation.

NASA Program Space Biology↗
NASA NTRS2022

Operational maize yield forecasts for Sub-Saharan Africa using Earth observation data and machine learning

Food insecurity continues to grow in Sub-Saharan Africa (SSA). In 2019, chronically malnourished people numbered nearly 240 million, or 20% of the population in SSA. Globally, numerous efforts have been made to anticipate potential droughts, crop conditions, and food shortages to foster improved food insecurity early warning and risk management. To support this goal, we develop an Earth Observation (EO) and machine-learning-based operational, subnational maize yield forecast system and evaluate its out-of-sample forecast skills during the growing seasons for Kenya, Somalia, Malawi, and Burkina Faso. In general, forecast skills improve substantially during the vegetative growth period (VP) and gradually during the reproductive development period (RP). Thus, mid-season assessment can provide effective early warning months before harvest. Skillful forecasts (Nash Sutcliffe Efficiency (NSE) > 0.6 and Mean Absolute Percentage Error (MAPE) < 20%) appear approximately two dekads after the VP; for example, they appear in May in Kenya and Somalia, January in Malawi, and July in Burkina Faso.

Donghoon Lee↗
NASA NTRS2000

A Parallel Genetic Algorithm for Automated Electronic Circuit Design

Parallelized versions of genetic algorithms (GAs) are popular primarily for three reasons: the GA is an inherently parallel algorithm, typical GA applications are very compute intensive, and powerful computing platforms, especially Beowulf-style computing clusters, are becoming more affordable and easier to implement. In addition, the low communication bandwidth required allows the use of inexpensive networking hardware such as standard office ethernet. In this paper we describe a parallel GA and its use in automated high-level circuit design. Genetic algorithms are a type of trial-and-error search technique that are guided by principles of Darwinian evolution. Just as the genetic material of two living organisms can intermix to produce offspring that are better adapted to their environment, GAs expose genetic material, frequently strings of 1s and Os, to the forces of artificial evolution: selection, mutation, recombination, etc. GAs start with a pool of randomly-generated candidate solutions which are then tested and scored with respect to their utility. Solutions are then bred by probabilistically selecting high quality parents and recombining their genetic representations to produce offspring solutions. Offspring are typically subjected to a small amount of random mutation. After a pool of offspring is produced, this process iterates until a satisfactory solution is found or an iteration limit is reached. Genetic algorithms have been applied to a wide variety of problems in many fields, including chemistry, biology, and many engineering disciplines. There are many styles of parallelism used in implementing parallel GAs. One such method is called the master-slave or processor farm approach. In this technique, slave nodes are used solely to compute fitness evaluations (the most time consuming part). The master processor collects fitness scores from the nodes and performs the genetic operators (selection, reproduction, variation, etc.). Because of dependency issues in the GA, it is possible to have idle processors. However, as long as the load at each processing node is similar, the processors are kept busy nearly all of the time. In applying GAs to circuit design, a suitable genetic representation 'is that of a circuit-construction program. We discuss one such circuit-construction programming language and show how evolution can generate useful analog circuit designs. This language has the desirable property that virtually all sets of combinations of primitives result in valid circuit graphs. Our system allows circuit size (number of devices), circuit topology, and device values to be evolved. Using a parallel genetic algorithm and circuit simulation software, we present experimental results as applied to three analog filter and two amplifier design tasks. For example, a figure shows an 85 dB amplifier design evolved by our system, and another figure shows the performance of that circuit (gain and frequency response). In all tasks, our system is able to generate circuits that achieve the target specifications.

Long, Jason D.↗
NASA NTRS2000

Gravity independence of seed-to-seed cycling in Brassica rapa

Growth of higher plants in the microgravity environment of orbital platforms has been problematic. Plants typically developed more slowly in space and often failed at the reproductive phase. Short-duration experiments on the Space Shuttle showed that early stages in the reproductive process could occur normally in microgravity, so we sought a long-duration opportunity to test gravity's role throughout the complete life cycle. During a 122-d opportunity on the Mir space station, full life cycles were completed in microgravity with Brassica rapa L. in a series of three experiments in the Svet greenhouse. Plant material was preserved in space by chemical fixation, freezing, and drying, and then compared to material preserved in the same way during a high-fidelity ground control. At sampling times 13 d after planting, plants on Mir were the same size and had the same number of flower buds as ground control plants. Following hand-pollination of the flowers by the astronaut, siliques formed. In microgravity, siliques ripened basipetally and contained smaller seeds with less than 20% of the cotyledon cells found in the seeds harvested from the ground control. Cytochemical localization of storage reserves in the mature embryos showed that starch was retained in the spaceflight material, whereas protein and lipid were the primary storage reserves in the ground control seeds. While these successful seed-to-seed cycles show that gravity is not absolutely required for any step in the plant life cycle, seed quality in Brassica is compromised by development in microgravity.

Flight Experiment↗
NASA NTRS2020

Impacts of 1.5 °C and 2.0 °C global warming above pre-industrial on potential winter wheat production of China

Keeping global temperatures below 2.0 °C above pre-industrial condition and pursuing efforts toward the more ambitious 1.5 °C goal in the late 21st century was the main target from the Paris Agreement in 2015. Here we assessed the likely challenges for the China’s winter wheat production under 1.5 °C and 2.0 °C increase of global temperature, with four wheat crop models (CERES-Wheat, Nwheat, WheatGrow, and APSIM-Wheat) and the latest climate projections from the Half a degree Additional warming, Prognosis and Projected Impacts project (HAPPI). Instead of using average “winter type” wheat cultivar, and same management and soil inputs for whole region, location-specific winter wheat cultivars with local agronomic information were calibrated for each of the representative wheat growing area of China, allowing a better spatial agronomic representation of the whole wheat planting area. The mean growing season temperature (GST) during the winter wheat vegetative stage was projected to increase by 0.6 to 1.4 °C for the 1.5 °C scenario, and 0.9 to 1.8 °C for the 2.0 °C scenario, while during the reproductive stage was decreased between 0 and 0.9 °C for the 1.5 °C scenario and -0.3 and 1.1 °C for the 2.0 °C scenario. Growing season duration (GSD) for the whole period was shortened by 6 to 15 days for the 1.5 °C scenario and 8 to 18 days for the 2.0 °C scenario, as a result of higher GST under global warming. Increase in GST and decrease in GSD was more obvious in the Southwest Subregion (SWS) than subregions in the north. The shortening GSD for the whole wheat growth period was mostly from the shortening vegetative period, as no appreciable difference in number of days from anthesis to maturity was found for the whole regions. Although there is variability among models, the indication is that wheat yields were projected to increase in the North Subregion (NS), the Huang-Huai Subregion (HHS), and the Middle-lower Researches of Yangzi River Subregion (MYS), but to decrease in the SWS under two warming scenarios. The effects of elevated CO2 concentration were mostly beneficial and tended to offset the negative impacts of increasing temperature at both global warming scenarios, with a rate of 7-14% yield increase per 100-ppm, except for locations with GST of baseline higher than 11 °C. Aggregating to regional wheat production, the total winter wheat production of China was projected to increase by 2.8% (1.6% to 3.0%, 25th percentile to 75th percentile) and 8.3% (7.0% to 9.6%, 25th percentile to 75th percentile) under 1.5 °C and 2.0 °C scenarios, and most of increase was observed in the north subregions due to the largest wheat planting area. Our results will lay the foundation for developing adaptation strategies to future climate change to ensure China and global wheat supply and food security.

Winter wheat↗
NASA NTRS2000

Influence of microgravity on ultrastructure and storage reserves in seeds of Brassica rapa L

Successful plant reproduction under spaceflight conditions has been problematic in the past. During a 122 d opportunity on the Mir space station, full life cycles of Brassica rapa L. were completed in microgravity in a series of three experiments in the Svet greenhouse. Ultrastructural and cytochemical analyses of storage reserves in mature dry seeds produced in these experiments were compared with those of seeds produced during a high-fidelity ground control. Additional analyses were performed on developing Brassica embryos, 15 d post pollination, which were produced during a separate experiment on the Shuttle (STS-87). Seeds produced on Mir had less than 20% of the cotyledon cell number found in seeds harvested from the ground control. Cytochemical localization of storage reserves in mature cotyledons showed that starch was retained in the spaceflight material, whereas protein and lipid were the primary storage reserves in ground control seeds. Protein bodies in mature cotyledons produced in space were 44% smaller than those in the ground control seeds. Fifteen days after pollination, cotyledon cells from mature embryos formed in space had large numbers of starch grains, and protein bodies were absent, while in developing ground control seeds at the same stage, protein bodies had already formed and fewer starch grains were evident. These data suggest that both the late stage of seed development and maturation are changed in Brassica by growth in a microgravity environment. While gravity is not absolutely required for any step in the plant life cycle, seed quality in Brassica is compromised by development in microgravity.

NASA Experiment Number 9600004↗