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All sourcesNASA NTRSDOE OSTI
DOE OSTI2025

Toward a sustainable circular economy of multilayer plastic films: Life cycle and techno-economic assessment with a focus on end-of-life treatment and multiple recovery cycles

This study presents a life cycle assessment (LCA) and techno-economic analysis (TEA) of end-of-life technologies for treating polyethylene–polyamide barrier film waste, focusing on quality degradation across recovery cycles. Novel treatment methods are experimentally validated, while others are drawn from literature and industry consultations. A displacement approach, assuming no quality loss, is first applied. Results show that solvent-based recycling via the solvent-targeted recovery and precipitation (STRAP) process outperforms alternatives across environmental indicators, reducing global warming potential (GWP) by 40% compared to landfilling. Incineration performs worst in most categories, particularly eutrophication (80% higher than landfilling), due to nitrogen emissions. Experimentally validated downcycling (pelletizing) proves more economically viable. The assumption of infinite recoverability is overly optimistic. To address this, we propose a mathematical framework accounting for a finite number of recovery cycles. This refined model shows reduced GWP and cost savings for solvent recovery, making its benefits less pronounced than initially estimated. Sensitivity and uncertainty analyses reveal strong dependence on recovered material quality and solvent recovery efficiency, underscoring the need for optimized process design. Finally, hotspot analysis identifies greenhouse gas emissions from the polyamide supply chain as the dominant GWP contributor. In conclusion, the results underscore potential trade-offs across pathways and show that solvent-based recovery’s sustainability depends heavily on process conditions.

36 MATERIALS SCIENCE↗
NASA NTRS2012

A 'Warm Formamide' Scenario for the Origins of Life Might Not Be so Hot Comment on 'Formamide and the Origin of Life' by E. Di Mauro Et Al.

In this review, Saladino et al. present an intriguing hypothesis surrounding the role of formamide in the originsof life on Earth, backed by experimental results supporting each step from formamide to RNA polymers [1]. The overall premise is that, from formamide and inorganic phosphate, RNA molecules over 100 nucleotides in length canbe produced. In addition, many carboxylic acids likely relevant to prebiotic metabolism are formed along the way. Thus, from a rather simple organic molecule that has been observed in outer space (formamide), you can generatemany of the compounds necessary for the origins of life. However, because high temperatures (160 C) are requiredfor the formamide reactions, it remains unclear where the warm formamide scenario could have occurred.Low-temperature, aqueous hydrogen counter to the observation that all protein-catalyzed ligation and polymerization reactions of RNA and DNA requireactivated substrates. Detailed mechanistic studies of the reported reactions are warranted and could provide important insights for understanding the chemistry behind the origins of life.Because the authors have produced many of the experimental results supporting their hypothesis, they coulddemonstrate the validity of their hypothesis by converting formamide into 100 nucleotide RNA oligomers, usingthe products of one reaction as the reactants for the next reaction, under specific conditions plausible on the pre-bioticEarth. Such a demonstration would represent a milestone for our understanding of the origins of life.cyanide-based prebiotic chemistry that we know actually happened has beenshown to produce many of the molecules invoked in the formamide hypothesis: amino acids, carboxylic acids, sugaracids, and nucleobases have all been found in meteorites recovered on Earth [e.g. [24]], providing a plausible routefor their synthesis and delivery. In contrast, a large portion of the formamide hypothesis is based on relatively high-temperature reactions. A plausible milieu for high-temperature reactions with concentrated formamide is yet to bedescribed, and is critical for this hypothesis to be validated. Hydrothermal vents are attractive heat sources, and the higher boiling point of formamide has been invoked as a mechanism to concentrate it from an aqueous solution.Unless the water can actually evaporate, however, there would be no net enrichment. For example, in the context of a deep-sea vent, any water removed by heating would be quickly replaced.Some of the individual reactions underpinning the present hypothesis [1] have been met with skepticism becausethey go against conventional wisdom. To name a few of the surprising results: the observation that nucleosides can beconverted to cyclic phosphates when heated in the presence of minerals and inorganic phosphate [5]; that 35 cGMPand cAMP nucleotides polymerize rapidly into RNA oligomers, even in the absence of monovalent counterions [6];and that end-to-end ligation reactions between RNA oligomers occur in essentially pure water, without requiring any activating groups or counterions [7,8]. Because the polymerization reactions are simply transesterification reactions,that they readily occur in the absence of cations makes one wonder why nearly all ribozyme-catalyzed transesterification reactions are metal-ion dependent; similarly, that the end-to-end ligation reactions do not require activation runs.

life↗
NASA NTRS2015

The Life Cycle Cost (LCC) of Life Support Recycling and Resupply

Brief human space missions supply all the crew's water and oxygen from Earth. The multiyear International Space Station (ISS) program instead uses physicochemical life support systems to recycle water and oxygen. This paper compares the Life Cycle Cost (LCC) of recycling to the LCC of resupply for potential future long duration human space missions. Recycling systems have high initial development costs but relatively low durationdependent support costs. This means that recycling is more cost effective for longer missions. Resupplying all the water and oxygen requires little initial development cost but has a much higher launch mass and launch cost. The cost of resupply increases as the mission duration increases. Resupply is therefore more cost effective than recycling for shorter missions. A recycling system pays for itself when the resupply LCC grows greater over time than the recycling LCC. The time when this occurs is called the recycling breakeven date. Recycling will cost very much less than resupply for long duration missions within the Earth-Moon system, such as a future space station or Moon base. But recycling would cost about the same as resupply for long duration deep space missions, such as a Mars trip. Because it is not possible to provide emergency supplies or quick return options on the way to Mars, more expensive redundant recycling systems will be needed.

recycling↗
NASA NTRSDate not supplied

The Icebreaker Life Mission to Mars: A Search for Biomolecular Evidence of Recent Life

The Icebreaker mission is the first mission to search for life signatures on Mars using biomolecular methods. Icebreaker lands in the ice-rich mid-latitudes of Mars and drills and collects samples down to at least 1 m depth to 1) search for molecular signatures of life and 2) assess the habitability of the icy regolith in the context of recent orbital cycles. Icebreaker is a NASA Discovery-class mission.

Mars Life Detection↗
DOE OSTI2026

Data for Rewiring Yeast Metabolism for Producing 2,3-Butanediol and Two Downstream Applications: Techno-Economic Analysis and Life Cycle Assessment of Methyl Ethyl Ketone (MEK) and Agricultural Biostimulant Production

Rising concerns for sustainability and global climate change have driven the development of sustainable production pathways for biofuels and chemicals from lignocellulosic biomass via integrated biological and chemical processes. We constructed an engineered Saccharomyces cerevisiae capable of producing 2,3-butanediol (2,3-BDO) from glucose without accumulating ethanol and glycerol, which hinder downstream processing of 2,3-BDO, through extensive metabolic reprogramming. Specifically, we introduced heterologous 2,3-BDO biosynthetic enzymes and deleted the major isozymes of ethanol and glycerol biosynthetic enzymes. In addition, we introduced an NAD+ regenerating Pyruvate-Malate (PM) cycle and enhanced the NAD+ regenerating capability of the PM cycle to resolve the redox imbalance from the deletion of ethanol and glycerol production pathways. The resulting engineered yeast produced 109.9 g/L of 2,3-BDO with a productivity of 1.0 g/L/h and a yield of 0.36 g/g glucose in a fed-batch fermentation. We also conducted techno-economic analysis (TEA) and life cycle assessment (LCA) of the production of methyl ethyl ketone (MEK) through catalytic dehydration of 2,3-BDO. A TEA based on the experimental results indicated that the minimum product selling price (MPSP) was estimated to be $1.90/kg. Regarding cradle-to-grave LCA, 100-year global warming potential (GWP100) and fossil energy consumption (FEC) were found to be 0.37 kg CO2 eq/kg and 3.1 MJ/kg, respectively. These results demonstrated the feasibility of cost-competitive and sustainable bio-based MEK production via yeast fermentation. In addition, we explored the possibility of using the fermentation broth containing 2,3-BDO as a biostimulant inducing drought tolerance in plants. As a result, the yeast 2,3-BDO fermentation broth can induce drought tolerance in Arabidopsis thaliana without a complicated purification process.

Economics↗
DOE OSTI2024

Characterization of Precipitation-Strengthening Heat-Resistant Austenitic Stainless Steels for Life-Prediction Modeling

In this study, the role of minor alloying additions in 347H stainless steels (UNS34709, ASTM A240/240M) on creep-rupture properties at 650-750°C and microstructure evolution during isothermal exposure at 750°C has been investigated, aiming to provide the experimental dataset as boundary conditions of physics-based modeling for material/component life prediction. Four different 347H heats containing various amounts of boron and nitrogen additions were prepared and evaluated. The combined additions of B and N are found to stabilize the strengthening secondary M23C6 carbides and retarding the transition from M23C6 to sigma phase precipitates during thermal exposure. The observed kinetics of microstructure evolution reasonably explains the improvement of creep-rupture properties of 347H stainless steels with the B and N additions.

Yamamoto, Yukinori↗
NASA NTRS1970

Development of a portable life support system and emergency life support pack

The design, development, and fabrication of a feasibility model of a breathing bag life support system for extravehicular activity are discussed. The breathing vest and back pack portable life support system contains connectors which allow external water and gas supply. At a metabolic rate of 2000 BTU per hour, the two low pressure bottles provide 27 minutes of breathing gas for a total filled system weight of 30.5 pounds.

Source record↗
NASA NTRS1978

Ductility normalized-strain-range partitioning life relations for creep-fatigue life predictions

Techniques utilizing strainrange partitioning may be used to estimate the effects of the environment on the high-temperature, low-cycle, creep-fatigue resistance of alloys. Three levels of ductility-normalized strainrange-partitioning life relations are discussed: (1) strainrange partitioning relations from ductility data, (2) strainrange partitioning relations scaled by ductility ratios, and (3) strainrange partitioning life relations with measured PP lines. The procedures have demonstrated good agreement with available creep-fatigue data.

Halford, G. R.↗
NASA NTRS1985

System analysis study of space platform and station accommodations for life sciences research facilities. Volume 2: Study results. Appendix D: Life sciences research facility requirements

The purpose of this requirements document is to develop the foundation for concept development for the Life Sciences Research Facility (LSRF) on the Space Station. These requirements are developed from the perspective of a Space Station laboratory module outfitter. Science and mission requirements including those related to specimens are set forth. System requirements, including those for support, are detailed. Functional and design requirements are covered in the areas of structures, mechanisms, electrical power, thermal systems, data management system, life support, and habitability. Finally, interface requirements for the Command Module and Logistics Module are described.

Wiley, Lowell F.↗
NASA NTRS1995

Life Sciences and Space Research 24 (4): Planetary Biology and Origins of Life; Topical Meeting of the COSPAR Interdisciplinary Scientific Commission F (Meeting F3) of the COSPAR Plenary Meeting, 29th, Washington, DC, Aug. 28-Sep. 5, 1992

The proceedings include sessions on extraterrestrial organic chemistry and the origins of life; life on Mars: past, present and future; planetary protection of Mars missions; chemical evolution on Titan; origins and early evolution of biological (a) energy transduction and membranes (b) information and catalysis; and carbon chemistry and isotopic fractionations in astrophysical environments.

Greenberg, J. M.↗
NASA NTRS2000

Life into Space: Space Life Sciences Experiments, Ames Research Center, Kennedy Space Center, 1991-1998, Including Profiles of 1996-1998 Experiments

We have now conducted space life sciences research for more than four decades. The continuing interest in studying the way living systems function in space derives from two main benefits of that research. First, in order for humans to engage in long-term space travel, we must understand and develop measures to counteract the most detrimental effects of space flight on biological systems. Problems in returning to the conditions of Earth must be kept to a manageable level. Second, increasing our understanding of how organisms function in the absence of gravity gives us new understanding of fundamental biological processes. This information can be used to improve human health and the quality of life on Earth.

Souza, Kenneth↗
NASA NTRS2002

Structural Life and Reliability Metrics: Benchmarking and Verification of Probabilistic Life Prediction Codes

Over the past two decades there has been considerable effort by NASA Glenn and others to develop probabilistic codes to predict with reasonable engineering certainty the life and reliability of critical components in rotating machinery and, more specifically, in the rotating sections of airbreathing and rocket engines. These codes have, to a very limited extent, been verified with relatively small bench rig type specimens under uniaxial loading. Because of the small and very narrow database the acceptance of these codes within the aerospace community has been limited. An alternate approach to generating statistically significant data under complex loading and environments simulating aircraft and rocket engine conditions is to obtain, catalog and statistically analyze actual field data. End users of the engines, such as commercial airlines and the military, record and store operational and maintenance information. This presentation describes a cooperative program between the NASA GRC, United Airlines, USAF Wright Laboratory, U.S. Army Research Laboratory and Australian Aeronautical & Maritime Research Laboratory to obtain and analyze these airline data for selected components such as blades, disks and combustors. These airline data will be used to benchmark and compare existing life prediction codes.

Litt, Jonathan S.↗
NASA NTRS2005

Life in the Atacama - Year 2: Geologic Reconnaissance Through Long-Range Roving and Implications on the Search for Life

The "Life in the Atacama" (LITA) project included two field trials during the 2004 field season, each of which lasted about a week. The remote science team had no prior knowledge of the local geology, and relied entirely on orbital images and rover-acquired data to make interpretations. The sites for these trials were in different locations, and are designated "Site B" and "Site C" respectively. The primary objective of the experiment is to develop and test the means to locate, characterize, and identify habitats and life remotely through long-range roving, which included field testing the rover, named Zoe. Zoe has onboard autonomous navigation for long-range roving, a plow to overturn rocks and expose near-surface rock materials, and high-resolution imaging, spectral, and fluorescence sampling capabilities. Highlights from the experiment included characterizing the geology in and near the landing ellipse, assessing pre-mission, satellite-based hypotheses, and improving the approach and procedures used by the remote and field teams for upcoming experiments through combined satellite, field-based, and microscopic perspectives and long-range roving.

Dohm, J. M.↗
NASA NTRS2015

[Redesign of the Spacesuit Long Life Battery and the Personal Life Support System Battery]

This fall I was working on two different projects that culminated into a redesign of the spacesuit LLB (long life battery). I also did some work on the PLSS (personal life support system) battery with EC. My first project was redlining the work instruction for completing DPAs (destructive physical analysis) on battery cells in the department. The purpose of this document is to create a standard process and ensure that the data in the same way no matter who carries out the analysis. I observed three DPAs, conducted one with help, and conducted two on my own all while taking notes on the procedure. These notes were used to write the final work instruction that will become is the department standard. My second project continued the work of the summer co-op before me. I was testing aluminum heat sinks for their ability to provide good thermal conduction and structural support during a thermal runaway event. The heat sinks were designed by the summer intern but there was not much time for testing before he left. We ran tests with a heater on the bottom of a trigger cell to try to drive thermal runaway and ensure that it will not propagate to adjacent cells. We also ran heat-to-vent tests in an oven to see if the assembly provided structural support and prevented sidewall rupture during thermal runaway. These tests were carried out at ESTA (energy systems test area) and are providing very promising results that safe, high performing (greater than 180 Wh/kg) designs are possible. My main project was a redesign of the LLB battery. Another summer intern did some testing and concluded that there was no simple fix to mitigate thermal runaway propagation hazards in the current design. The only option was a clean sheet redesign of the battery. I was given a volume and ideal energy density and the rest of the design was up to me. First, I created new heat sink banks in Creo using the information gathered in the metal heat sink tests from the summer intern. After this, I made capture plates to hold the cells in place and I worked on nickel bussings for the electrical connections between the cells. Finally, I designed the test box enclosure that included sections for flame arresting materials. The battery brick design, which is the heart of the battery, promises to become the first for a manned spacecraft application to achieve greater than 180 Wh/kg. My work in redlining the DPA work instructions will also be used in selecting the cells for the battery. We had a few options of cells that would provide the necessary power output and needed to make a choice. We repeatedly charged and discharged cells for around a month until they went through 100 lifecycles. The plan is to compare the DPA results on fresh and cycled cells from each manufacturer to see if cycling introduces any differences. After the complete LLB design was approved, the parts were ordered and testing should begin the first week of December. Some of my side projects included working on the CAD data for the PLSS with EC and attending the NASA Aerospace Battery Workshop in Huntsville. I was also a member of the Tours and Lectures Committee for the USRA and Pathways interns. I coordinated Apollo Evening and was on the committee for touring KSC and seeing an Atlas 5 launch. I really enjoyed my time at JSC and I would like to continue working for NASA or another aerospace company in the future. I have worked other internships prior to this, but I think the heavy research and development focus is the best fit for me. I originally thought I would need to go to grad school to work in an environment like this, but I now see it is possible with a bachelor’s degree and hard work. I would like to go into the workforce and maybe continue my education with night classes.

Scharf, Stephanie↗
NASA NTRS2016

Enceladus Life Finder: the Search for Life in a Habitable Moon

Enceladus is one of the most intriguing bodies in the solar system. In addition to having one of the brightest and youngest surfaces, this small Saturnian moon was recently discovered to have a plume erupting from its south polar terrain and a global subsurface ocean. The Cassini Mission discovered organics and nitrogen-bearing molecules in the plume, as well as salts and silicates that strongly suggest ocean water in contact with a rocky core. However, Cassini's instruments lack sufficient resolution and mass range to determine if these organics are of biotic origin. The Enceladus Life Finder (ELF) is a Discovery-class mission that would use two state-of-the-art mass spectrometers to target the gas and grains of the plume and search for evidence of life in this alien ocean.

Saturn↗
NASA NTRS2019

Biosignatures of Past Life are Also Relevant to the Search for Extant Life

A biosignature (a “definitive biosignature” or DBS) is an object, substance pattern and/or process whose origin requires a biological agent. A potential biosignature (PBS) is a feature that might have a biological origin and thus compels investigators to gather more data before determining the presence or absence of life. The usefulness of a PBS is determined not only by the probability that life created it but also by the improbability that nonbiological processes produced it. Habitable planetary environments create nonbiological features that can mimic biosignatures, so these environments must be characterized to the extent necessary to confirm the presence of DBS. Environments also must have allowed biosignatures to be preserved and to remain amenable to detection.

Des Marais, D. J.↗
NASA NTRSDate not supplied

A Simulated Drilling Mission to Search for Bio-molecular Signatures of Life on Mars Performed in the Atacama Desert (Chile): Demonstrating Drilling, Sample Handling, and Life-Detection Instruments Remotely Operated with Mission-like Protocols

The search for evidence of life on Mars requires accessing materials that are protected from the oxidizing and irradiated conditions at the surface. Mars rovers Curiosity and Perseverance have performed shallow (few cm) drilling to access relevant samples. The upcoming ExoMars mission will acquire samples from up to 2 meters depth. Field experiments in Mars analog sites help prepare for this and other future deep drilling missions. In Sept. 2019 the ARADS (Atacama Rover Astrobiology Drilling Studies project (Glass et al. 2022) conducted a rover-based drilling mission to search for bio-molecular evidence of life in a Mars analog site in Atacama Chile.

Carol R. Stoker↗
NASA NTRSDate not supplied

A Simulated Drilling Mission to Search for Biomolecular Signatures of Life on Mars Performed in the Atacama Desert (Chile): Demonstrating Drilling, Sample Handling, and Life-Detection Instruments Remotely Operated with Mission-like Protocols

The search for evidence of life on Mars requires accessing materials that are protected from the oxidizing and irradiated conditions at the surface. Mars rovers Curiosity and Perseverance have performed shallow (few cm) drilling to access relevant samples. The upcoming ExoMars mission will acquire samples from up to 2 meters depth. Field experiments in Mars analog sites help prepare for this and other future deep drilling missions. In Sept. 2019 the ARADS (Atacama Rover Astrobiology Drilling Studies project (Glass et al. 2022) conducted a roverbased drilling mission to search for biomolecular evidence of life in a Mars analog site in Atacama, Chile.

Carol R. Stoker↗