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At least 55 records · Page 3

Accumulation of trace elements in soil and fauna within a site historically contaminated with coal combustion residues

Legacy contaminants tied to energy production are a worldwide concern. Coal combustion residues (CCRs) contain high concentrations of potentially toxic trace elements such as arsenic (As), mercury (Hg), and selenium (Se), which can persist for decades after initial contamination. Coal combustion residue disposal methods, including aquatic settling basins and landfills, can facilitate environmental exposure through intentional and accidental releases. Wildlife exposed to CCRs can experience numerous deleterious effects, such as on development, reproduction, and survival. In the current study, we quantified and compared concentrations of As, Hg, Se, and strontium (Sr) within soils and target fauna (three vertebrate and three invertebrate taxa) from a CCR-contaminated site and a reference site within the U.S. Department of Energy’s Savannah River Site, South Carolina, United States. Our objectives were to (1) compare current concentrations of tested elements in soil and resident fauna to levels from our reference site, (2) assess natural attenuation of elements in soils by comparing current concentrations to historic levels, and (3) evaluate the biomagnification potential of the elements measured via body burden and trophic position correlations among fauna. Here, element concentrations were higher in contaminated soils than reference soils; however, concentrations in 2022 were unchanged from concentrations measured in 2003, suggesting no natural attenuation of tested elements. Additionally, target fauna had elevated As, Se, and Sr levels in comparison to reference samples. A positive correlation was observed in southern toads between Sr concentrations and trophic position, as assessed by nitrogen stable isotope ratios, suggesting potential for biomagnification of Sr within our study system. Collectively, our results demonstrate that legacy contaminants are still present and bioaccumulate in a diversity of taxa in a CCR-contaminated site that has not received effluents in more than 50 years, suggesting monitoring programs in CCR-contaminated sites should be maintained long term in the absence of remediation.

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTAN↗

Climate, food and humans predict communities of mammals in the United States

Abstract Aim The assembly of species into communities and ecoregions is the result of interacting factors that affect plant and animal distribution and abundance at biogeographic scales. Here, we empirically derive ecoregions for mammals to test whether human disturbance has become more important than climate and habitat resources in structuring communities. Location Conterminous United States. Time Period 2010–2021. Major Taxa Studied Twenty‐five species of mammals. Methods We analysed data from 25 mammal species recorded by camera traps at 6645 locations across the conterminous United States in a joint modelling framework to estimate relative abundance of each species. We then used a clustering analysis to describe 8 broad and 16 narrow mammal communities. Results Climate was the most important predictor of mammal abundance overall, while human population density and agriculture were less important, with mixed effects across species. Seed production by forests also predicted mammal abundance, especially hard‐mast tree species. The mammal community maps are similar to those of plants, with an east–west split driven by different dominant species of deer and squirrels. Communities vary along gradients of temperature in the east and precipitation in the west. Most fine‐scale mammal community boundaries aligned with established plant ecoregions and were distinguished by the presence of regional specialists or shifts in relative abundance of widespread species. Maps of potential ecosystem services provided by these communities suggest high herbivory in the Rocky Mountains and eastern forests, high invertebrate predation in the subtropical south and greater predation pressure on large vertebrates in the west. Main Conclusions Our results highlight the importance of climate to modern mammals and suggest that climate change will have strong impacts on these communities. Our new empirical approach to recognizing ecoregions has potential to be applied to expanded communities of mammals or other taxa.

Kays, Roland↗

Water Temperature, Prey Concentration and Salmonid Density Influence Daily Growth of Wild Juvenile Salmonids in Tributaries of the Upper Salmon River, Idaho ( USA )

ABSTRACT Theory, experiments and field studies indicate that the somatic growth rate of freshwater consumers is shaped by the individual, additive and multiplicative effects of multiple factors, including consumer size and condition, temperature, prey resources and biotic interactions. While our understanding of how these factors affect wild populations of freshwater consumers is improving, the topic remains poorly studied, especially with respect to mobile species. Here, we report on an 8‐year, seven‐stream ( n = 49 stream‐year combinations) observational study examining the individual and interactive effects of invertebrate prey concentration (F, mg/m 3 ), mean daily water temperature (T, °C) and juvenile Chinook salmon ( Oncorhynchus tshawytscha ) density (D, fish/100 m 2 ) on summer daily growth rates (%/d) of mobile, anadromous, juvenile Chinook salmon (age‐0+, n = 382) and sub‐yearling (age‐0+, n = 61) and yearling (age‐1+) steelhead trout ( O. mykiss , n = 70) rearing in cold (mean daily summer: 12.1°C, range: 4.2°C–16.7°C) mountain tributaries of the Salmon River basin in central Idaho (USA). AIC c model selection indicated that daily juvenile salmonid growth positively correlated with water temperature, prey biomass concentration, local juvenile Chinook density and the interaction between water temperature and food but with species and age‐specific differences. Water temperature was a covariate in all top‐ranked models, with daily growth (%/day) rate increasing (0.05%–0.23%/d) linearly with mean daily summer water temperature. In addition to a direct positive relationship with daily growth rate, there was evidence that prey concentration positively interacted with water temperature to accelerate daily growth (F × T). The positive relationship between juvenile salmonid daily growth rate and juvenile Chinook density is difficult to explain and could result from confounding factors. The individual success observed in these streams may contribute to population‐level benefits for the focal consumers, as prey‐rich, warm summers may result in larger individuals with higher energy reserves at the end of the summer/autumn growing season, contributing to improved overwinter survival. Our results, taken in combination with evidence from models, experiments and observational studies, have climate change implications. Current and predicted increases in water temperature will necessitate higher rates of prey consumption by aquatic ectothermic consumers to offset accelerated metabolic demands. Thus, to improve the resilience of mobile freshwater consumers in a warming climate, we suggest that natural resource managers not only consider physical and chemical habitat conditions but also biotic conditions, including the spatiotemporal quantity and quality of prey resources.

Kiffney, Peter. M. [Fish Ecology, Northwest Fisher↗

Intestinal microbiome profile of the brown rock sea cucumber ( Holothuria glaberrima ) using ITS and 16S rDNA amplicons from direct mechanical, enzymatic, and chemical metagenomic extraction

Using direct mechanical, enzymatic, and chemical extraction methods, the intestinal microbiome of the marine invertebrate Holothuria glaberrima was obtained. ITS and 16S rDNA regions were sequenced to enrich and investigate the prokaryotic and fungal diversity profiles from different anatomical regions within the sea cucumber’s intestinal biology.

16S↗

Ecological Benchmark for Chemicals

The Ecological Benchmark Tool dataset serves as a comprehensive repository of benchmarks designed to assess ecological risks at contaminated sites. This tool facilitates the evaluation of various environmental media and contaminants, supporting regulatory compliance and ecological protection. Benchmarks are available for air, biota, sediment, soil, and surface water. The dataset also provides species-specific benchmarks for fish, plants, birds, mammals, and invertebrates. Users can select benchmark sources, media, individual chemicals, and retrieve results in tabular or spreadsheet formats for analysis. Benchmarks are derived from authoritative sources, including government agencies, scientific councils, and academic publications. The dataset supports ecological risk assessments, regulatory decision-making, and environmental planning, with tools for benchmarking against chemical thresholds, sensitive species protection, and habitat impact evaluations. This structured approach ensures a robust evaluation of ecological risks tailored to site-specific and regulatory needs.

Stewart, Debra [Oak Ridge National Laboratory (ORN↗

Ecological Benchmark for Radionuclides

The Ecological Benchmark Tool for radionuclides dataset serves as a comprehensive repository of benchmarks designed to assess ecological risks at contaminated sites. This tool facilitates the evaluation of various environmental media and contaminants, supporting regulatory compliance and ecological protection. Benchmarks are available for sediment, soil, and surface water. The dataset also provides species-specific benchmarks for fish, plants, birds, mammals, and invertebrates. Users can select benchmark sources, media, individual radionuclides, and retrieve results in tabular or spreadsheet formats for analysis. Benchmarks are derived from authoritative sources, including government agencies, scientific councils, and academic publications. The dataset supports ecological risk assessments, regulatory decision-making, and environmental planning, with tools for benchmarking against radiological thresholds, sensitive species protection, and habitat impact evaluations. This structured approach ensures a robust evaluation of ecological risks tailored to site-specific and regulatory needs.

Stewart, Debra [Oak Ridge National Laboratory (ORN↗

Exploiting bacterial effector proteins to uncover evolutionarily conserved antiviral host machinery

Arboviruses are a diverse group of insect-transmitted pathogens that pose global public health challenges. Identifying evolutionarily conserved host factors that combat arbovirus replication in disparate eukaryotic hosts is important as they may tip the balance between productive and abortive viral replication, and thus determine virus host range. Here, we exploit naturally abortive arbovirus infections that we identified in lepidopteran cells and use bacterial effector proteins to uncover host factors restricting arbovirus replication. Bacterial effectors are proteins secreted by pathogenic bacteria into eukaryotic hosts cells that can inhibit antimicrobial defenses. Since bacteria and viruses can encounter common host defenses, we hypothesized that some bacterial effectors may inhibit host factors that restrict arbovirus replication in lepidopteran cells. Thus, we used bacterial effectors as molecular tools to identify host factors that restrict four distinct arboviruses in lepidopteran cells. By screening 210 effectors encoded by seven different bacterial pathogens, we identify several effectors that individually rescue the replication of all four arboviruses. We show that these effectors encode diverse enzymatic activities that are required to break arbovirus restriction. We further characterize Shigella flexneri-encoded IpaH4 as an E3 ubiquitin ligase that directly ubiquitinates two evolutionarily conserved proteins, SHOC2 and PSMC1, promoting their degradation in insect and human cells. We show that depletion of either SHOC2 or PSMC1 in insect or human cells promotes arbovirus replication, indicating that these are ancient virus restriction factors conserved across invertebrate and vertebrate hosts. Collectively, our study reveals a novel pathogen-guided approach to identify conserved antimicrobial machinery, new effector functions, and conserved roles for SHOC2 and PSMC1 in virus restriction.

59 BASIC BIOLOGICAL SCIENCES↗

U.S. Pacific Coast Workshop Report on Preconstruction Research Recommendations (U.S. Offshore Wind Synthesis of Environmental Effects Research (SEER) Project)

In May 2022, the U.S. Offshore Wind Synthesis of Environmental Effects Research (SEER) project team hosted a stakeholder workshop focused on preconstruction (baseline) research needs for potential floating offshore wind (OSW) energy development on the U.S. Pacific Coast, including California, Oregon, and Washington. Prior to the workshop, the SEER team developed a set of initial synthesized research recommendations that were identified based on a review of relevant, publicly available resources and with advisory group input. The workshop covered three marine life breakout groups on subsequent days to discuss research recommendations related to 1) marine mammals and sea turtles, 2) fish and invertebrates, and 3) birds and bats. As part of the workshop, over a hundred participants from the public and private sectors provided feedback on various aspects of the initial research recommendations, including associated data and knowledge gaps, benefits/limitations of available methods and technologies, and technological advancements or infrastructure needed to address the recommendation. Approximately 1,000 total comments were received on the workshop MURAL boards and were synthesized in this report. Based on workshop feedback, SEER developed a final database of over 500 specific research recommendations based on more than 40 resources. In Fall 2022, the full database and a tool with updated synthesized research recommendations were disseminated on Tethys (https://tethys.pnnl.gov) to assist with informing future funding opportunities and research programming. There is a continued need to improve awareness of the potential environmental effects, monitoring technologies, and management strategies for floating OSW energy development on the U.S. Pacific Coast. Coordination of these activities will require the sustained involvement of multiple stakeholders from across sectors. Beyond the baseline considerations discussed in this workshop, future state-of-the-science activities should be planned to consider research needs across wind energy life cycle phases for all relevant wildlife taxa and associated habitat and ecosystem processes.

17 WIND ENERGY↗

Larval connectivity for European green crab management in the Salish Sea and surrounding waters

The presence of invasive species is a growing concern in coastal marine ecosystems because of their adverse effects on biodiversity. The European green crab Carcinus maenas (EGC) is a small crab inhabiting inshore areas. Although it is native to the Northeast Atlantic Ocean and Baltic Sea, its distribution has expanded to North America, where it is an invasive species. Its main food sources are small invertebrate species that support valuable fisheries in the USA. The first presence of EGC in northern Washington was observed around 20 yr ago, along the Pacific Coast of the USA. Recently, EGC has been detected throughout the Salish Sea (Washington, USA, and British Columbia, Canada) wherein spread dynamics are unknown. The overall distribution of EGC is mainly driven by larval dispersal and, in the Salish Sea and surrounding waters, the assessment of EGC population dynamics is essential to understand its migration patterns and prevent its future expansion. To investigate the dispersal patterns of EGC larvae, a larval dispersal model was developed which couples a regional model of hydrodynamic circulation with an individual-based model of ichthyoplankton dynamics. Simulations were performed over 9 yr (2013-2022) to analyze average larval transport trends in the Salish Sea and surrounding waters, interannual variability of EGC larval connectivity, and the influence of larval behavior on connectivity patterns. Lastly, areas were identified to inform invasive species management moving forward. The prediction of likely sources and settlement locations of EGC larvae from the model will help improve the management of the population in the Salish Sea and surrounding waters.

60 APPLIED LIFE SCIENCES↗

Biological response of eelgrass epifauna, Taylor's Sea hare ( Phyllaplysia taylori ) and eelgrass isopod ( Idotea resecata ), to elevated ocean alkalinity

Abstract. Marine carbon dioxide removal (mCDR) approaches are under development to mitigate the effects of climate change by sequestering carbon in stable reservoirs, with the potential co-benefit of local reductions in coastal acidification impacts. One such method is ocean alkalinity enhancement (OAE). A specific OAE method is the generation of aqueous alkalinity via electrochemistry to enhance the alkalinity of the receiving water by the extraction of acid from seawater, thereby avoiding the issues of solid dissolution kinetics and the release of impurities into the ocean from alkaline minerals. While electrochemical acid extraction is a promising method for increasing the carbon dioxide sequestration potential of the ocean, the biological effects of increasing seawater alkalinity and pH within an OAE project site are relatively unknown. This study aims to address this knowledge gap by testing the effects of increased pH and alkalinity, delivered in the form of aqueous NaOH, on two eelgrass epifauna in the US Pacific Northwest, Taylor's sea hare (Phyllaplysia taylori) and eelgrass isopod (Idotea resecata), chosen for their ecological importance as salmon prey and for their role in eelgrass ecosystems. Four-day experiments were conducted in closed bottles to allow measurements of the evolution of carbonate species throughout the experiment, with water refreshed twice daily to maintain elevated pH, across pHNBS (NBS standard scale) treatments ranging from 7.8 to 9.3. Sea hares experienced mortality in all pH treatments, ranging from 37 % mortality at pHNBS 7.8 to 100 % mortality at pHNBS 9.3. Isopods experienced lower mortality rates in all treatment groups, ranging from 13 % at pHNBS 7.8 to 21 % at pHNBS 9.3, which did not significantly increase with higher pH treatments. These experiments represent an extreme of constant exposure to elevated pH and alkalinity, which should be considered in the context of both the natural variation and the dilution of alkalinity experienced by marine communities across an OAE project site. Different invertebrate species will likely have different responses to increased pH and alkalinity, depending on their physiological vulnerabilities. Investigation of the potential vulnerabilities of local marine species will help inform the decision-making process regarding mCDR planning and permitting.

marine carbon dioxide removal↗

Feeding

Nutritional biology and ecology of various marine invertebrates - suspension feeding

HYDROBIOLOGY↗

Algal mutualism

Symbiosis between unicellular algae and marine invertebrates

ALGAE↗

Reference earth orbital research and applications investigations (blue book). Volume 8: Life sciences

The functional program element for the life sciences facilities to operate aboard manned space stations is presented. The life sciences investigations will consist of the following subjects: (1) medical research, (2) vertebrate research, (3) plant research, (4) cells and tissue research, (5) invertebrate research, (6) life support and protection, and (7) man-system integration. The equipment required to provide the desired functional capability for the research facilities is defined. The goals and objectives of each research facility are described.

Source record↗