Venus Global Reference Atmospheric Model (Venus-GRAM) Current and Future Upgrades
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atmospheric models↗
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Venus Global Reference Atmospheric Model (Venus-GRAM) Overview and Future Upgrades
Explore the source record for details and available documents.
atmospheric models↗
Meteorite Dunite Breccia MIL 03443: A Probable Crustal Cumulate Closely Related to Diogenites from the HED Parent Asteroid
There are numerous types of differentiated meteorites, but most represent either the crusts or cores of their parent asteroids. Ureilites, olivine-pyroxene-graphite rocks, are exceptions; they are mantle restites [1]. Dunite is expected to be a common mantle lithology in differentiated asteroids. In particular, models of the eucrite parent asteroid contain large volumes of dunite mantle [2-4]. Yet dunites are very rare among meteorites, and none are known associated with the howardite, eucrite, diogenite (HED) suite. Spectroscopic measurements of 4 Vesta, the probable HED parent asteroid, show one region with an olivine signature [5] although the surface is dominated by basaltic and orthopyroxenitic material equated with eucrites and diogenites [6]. One might expect that a small number of dunitic or olivine-rich meteorites might be delivered along with the HED suite. The 46 gram meteoritic dunite MIL 03443 (Fig. 1) was recovered from the Miller Range ice field of Antarctica. This meteorite was tentatively classified as a mesosiderite because large, dunitic clasts are found in this type of meteorite, but it was noted that MIL 03443 could represent a dunite sample of the HED suite [7]. Here I will present a preliminary petrologic study of two thin sections of this meteorite.
Mittlefehldt, David W.↗
Mars-GRAM Support for the Mars Ascent Vehicle
Mars sample return is a bold concept, which entails gathering a varied, scientifically-relevant collection of Martian rock core samples and bringing them to Earth for analysis. To support this endeavor, the Marshall Space Flight Center (MSFC) is developing the Mars Ascent Vehicle (MAV), which is responsible for getting the collected samples off the planet. The MAV Preliminary Architecture Assessment (PAA) study is designing two vehicle architectures based on different propulsion configurations: a two-stage solid-solid concept, and a hybrid concept. Given different thrust profiles for the two configurations, each concept uses a unique trajectory to reach the same orbit. In support of the PAA, The MSFC Natural Environments Branch (EV44) was asked to produce tables of atmospheric parameters along each of the two trajectories. The Mars Global Reference Atmospheric Model (Mars-GRAM) is an EV44 tool that is ideally suited for this analysis. Mars-GRAM will continue supporting MAV development in future design cycles.
Burns, K. L.↗
Airborne Radar for sUAS Sense and Avoid
A primary challenge for the safe integration of small UAS operations into the National Airspace System (NAS) is traffic deconfliction, both from manned and unmanned aircraft. The UAS Traffic Management (UTM) project being conducted at the National Aeronautics and Space Administration (NASA) considers a layered approach to separation provision, ranging from segregation of operations through airspace volumes (geofences) to autonomous sense and avoid (SAA) technologies for higher risk, densely occupied airspace. Cooperative SAA systems, such as Automatic Dependent Surveillance-Broadcast (ADS-B) and/or vehicle-to-vehicle communication systems provide significant additional risk mitigation but they fail to adequately mitigate collision risks for non-cooperative (non-transponder equipped) airborne aircraft. The RAAVIN (Radar on Autonomous Aircraft to Verify ICAROUS Navigation) flight test being conducted by NASA and the Mid-Atlantic Aviation Partnership (MAAP) was designed to investigate the applicability and performance of a prototype, commercially available sUAS radar to detect and track non-cooperative airborne traffic, both manned and unmanned. The radar selected for this research was a Frequency Modulated Continuous Wave (FMCW) radar with 120 degree azimuth and 80 degree elevation field of view operating at 24.55GHz center frequency with a 200 MHz bandwidth. The radar transmits 2 watts of power thru a Metamaterial Electronically Scanning Array antenna in horizontal polarization. When the radar is transmitting, personnel must be at least 1 meter away from the active array to limit nonionizing radiation exposure. The radar physical dimensions are 18.7cm by 12.1cm by 4.1cm and it weighs less than 820 grams making it well suited for installation on small UASs. The onboard, SAA capability, known as ICAROUS, (Independent Configurable Architecture for Reliable Operations of Unmanned Systems), developed by NASA to support sUAS operations, will provide autonomous guidance using the traffic radar tracks from the onboard radar. The RAAVIN set of studies will be conducted in three phases. The first phase included outdoor, ground-based radar evaluations performed at the Virginia Tech’s Kentland Farm testing range in Blacksburg, VA. The test was designed to measure how well the radar could detect and track a small UAS flying in the radar’s field of view. The radar was used to monitor 5 test flights consisting of outbound, inbound and crossing routes at different ranges and altitudes. The UAS flown during the ground test was the Inspire 2, a quad copter weighing less than 4250 grams (10 pounds) at maximum payload. The radar was set up to scan and track targets over its full azimuthal field of view from 0 to 40 degrees in elevation. The radar was configured to eliminate tracks generated from any targets located beyond 2000 meters from the radar and moving at velocities under 1.45 meters per second. For subsequent phases of the study the radar will be integrated with a sUAS platform to evaluate its performance in flight for SAA applications ranging from sUAS to manned GA aircraft detections and tracking. Preliminary data analysis from the first outdoor ground tests showed the radar performed well at tracking the vehicle as it flew outbound and repeatedly maintained a track out to 1000 meters (maximum 1387 meters) until the vehicle slowed to a stop to reverse direction to fly inbound. As the Inspire flew inbound tracks from beyond 800 meters, a reacquisition time delay was consistently observed between when the Inspire exceeds a speed of 1.45 meters per second and when the radar indicated an inbound target was present and maintained its track. The time delay varied between 6 seconds to over 37 seconds for the inbound flights examined, and typically resulted in about a 200 meter closure distance before the Inspire track was maintained. The radar performed well at both acquiring and tracking the vehicle as it flew crossing routes out past 400 meters across the azimuthal field of view. The radar and ICAROUS software will be integrated and flown on a BFD-1400-SE8-E UAS during the next phase of the RAAVIN project. The main goal at the conclusion of this effort is to determine if this radar technology can reliably support minimum requirements for SAA applications of sUAS. In particular, the study will measure the range of vehicle detections, lateral and vertical angular errors, false and missed/late detections, and estimated distance at closest point of approach after an avoidance maneuver is executed. This last metric is directly impacted by sensor performance and indicates its suitability for the task.
Szatkowski, George N.↗
Characterization of viable bacteria from Siberian permafrost by 16S rDNA sequencing
Viable bacteria were found in permafrost core samples from the Kolyma-Indigirka lowland of northeast Siberia. The samples were obtained at different depths; the deepest was about 3 million years old. The average temperature of the permafrost is -10 degrees C. Twenty-nine bacterial isolates were characterized by 16S rDNA sequencing and phylogenetic analysis, cell morphology, Gram staining, endospore formation, and growth at 30 degrees C. The majority of the bacterial isolates were rod shaped and grew well at 30 degrees C; but two of them did not grow at or above 28 degrees C, and had optimum growth temperatures around 20 degrees C. Thirty percent of the isolates could form endospores. Phylogenetic analysis revealed that the isolates fell into four categories: high-GC Gram-positive bacteria, beta-proteobacteria, gamma-proteobacteria, and low-GC Gram-positive bacteria. Most high-GC Gram-positive bacteria and beta-proteobacteria, and all gamma-proteobacteria, came from samples with an estimated age of 1.8-3.0 million years (Olyor suite). Most low-GC Gram-positive bacteria came from samples with an estimated age of 5,000-8,000 years (Alas suite).
Non-NASA Center↗
Mars Global Surveyor: Aerobraking and Observations Support Using a Mars Global Circulation Model
This is a Final Report for a Joint Research Interchange (JRI) between NASA Ames Research Center and San Jose State University, Department of Meteorology. Using a global atmospheric circulation model for Mars, the focus of this JRI has been to provide support for the Mars Global Surveyor (MGS) spacecraft aerobraking activities and interpretation guidance of preliminary observations. The primary atmospheric model applied in this investigation has been a high-top version of the NASA Ames Mars general circulation model (MGCM). Comparisons with an atmospheric model designed primarily for engineering purposes (Mars-GRAM) has also been carried out. From a suite of MGCM simulations, we have assessed plausible spatial and temporal variability in atmospheric density at high altitudes (e.g., 70-110 km) for seasonal dates and locations during Phase I aerobraking. Diagnostic tools have been developed to analyze circulation fields from the MGCM simulations, and these tools have been applied in the creation of a Mars climate catalogue database. Throughout Phase I aerobraking activities, analysis products have been provided to the MGS aerobraking atmospheric advisory group (AAG). Analyses of circulation variability at the coupling level between the MGCM and a Mars thermospheric global circulation model (MTGCM) has also been assessed. Finally, using a quasi-geostrophic dynamical formulation with the MGCM simulations, diagnosis of breaking planetary (Rossby) waves in Mars' middle atmosphere has been carried out. Titles of papers presented at scientific workshops and seminars, and a publication in the scientific literature are provided.
Hollingsworth, Jeffery L.↗
Mars Global Surveyor: Aerobraking and Observations Support Using a Mars Global Circulation Model
This is a Final Report for a Joint Research Interchange (JRI) between NASA Ames Research Center and San Jose State University, Department of Meteorology. Using a global atmospheric circulation model for Mars, the focus of this JRI has been to provide support for the Mars Global Surveyor (MGS) spacecraft aerobraking activities and interpretation guidance of preliminary observations. The primary atmospheric model applied in this investigation has been a high-top version of the NASA Ames Mars general circulation model (MGCM). Comparisons with an atmospheric model designed primarily for engineering purposes (Mars-GRAM) has also been carried out. From a suite of MGCM simulations, we have assessed plausible spatial and temporal variability in atmospheric density at high altitudes (e.g., 70-1 10 km) for seasonal dates and locations during Phase 1 aerobraking. Diagnostic tools have been developed to analyze circulation fields from the MGCM simulations, and these tools have been applied in the creation of a Mars climate catalogue database. Throughout Phase I aerobraking activities, analysis products have been provided to the MGS aerobraking atmospheric advisory group (AAG). Analyses of circulation variability at the coupling level between the MGCM and a Mars thermospheric global circulation model (MTGCM) has also been assessed. Finally, using a quasi-geostrophic dynamical formulation with the MGCM simulations, diagnosis of breaking planetary (Rossby) waves in Mars middle atmosphere has been carried out. Titles of papers presented at scientific workshops and seminars, and a publication in the scientific literature are provided.
Hollingsworth, Jeffery L.↗
Mars Global Surveyor: Aerobraking and Observations Support Using a Mars Global Circulation Model
This is a Final Report for a Joint Research Interchange (JRI) between NASA Ames Research Center and San Jose State University, Department of Meteorology. Using a global atmospheric circulation model for Mars, the focus of this JRI has been to provide support for the Mars Global Surveyor (MGS) spacecraft aerobraking activities and interpretation guidance of preliminary observations. ne primary atmospheric model applied in this investigation has been a high-top version of the NASA Ames Mars general circulation model (MGCM). Comparisons with an atmospheric model designed primarily for engineering purposes (Mars-GRAM) has also been carried out. From a suite of MGCM simulations, we have assessed plausible spatial and temporal variability in atmospheric density at high altitudes (e.g., 70-110 km) for seasonal dates and locations during Phase 1 aerobraking. Diagnostic tools have been developed to analyze circulation fields from the MGCM simulations, and these tools have been applied in the creation of a Mars climate catalogue database. Throughout Phase 1 aerobraking activities, analysis products have been provided to the MGS aerobraking atmospheric advisory group (AAG). Analyses of circulation variability at the coupling level between the MGCM and a Mars thermospheric global circulation model (MTGCM) has also been assessed. Finally, using a quasi-geostrophic dynamical formulation with the MGCM simulations, diagnosis of breaking planetary (Rossby) waves in Mars' middle atmosphere has been carried out. Titles of papers presented at scientific workshops and seminars, and a publication in the scientific literature are provided.
Hollingsworth, Jeffery L.↗
Optimizing Cupriavidus necator H16 as a host for aerobic C1 conversion
Biological systems capable of converting CO 2 or CO 2 -derived, single-carbon (C1) compounds can be used to reduce or reverse carbon emissions while establishing a circular bioeconomy to provide sustainable sources of the fuels, foods, and materials humanity relies on. A robust bioeconomy will rely upon a variety of microorganisms capable of assimilating C1 compounds and converting them to valuable products at industrial scale. While anaerobic microbes are ideal hosts for production of short-chain acids and alcohols, microbes capable of aerobic respiration are well suited for biosynthesis of higher molecular weight products. One such organism is the gram-negative soil bacterium Cupriavidus necator, which has been utilized in commercial production of biopolymers for decades. More recently, its capability of robust, aerobic growth on CO 2 has inspired research efforts that have advanced it toward becoming one of the leading bacterial hosts for C1-based biomanufacturing. This review highlights those efforts in the context of the characteristics that have historically made C. necator an excellent host for industrial bioconversion processes: its metabolic versatility, ability to grow rapidly to high cell densities, and genetic amenability.
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
Containerless Processing in Reduced Gravity Using the TEMPUS Facility
Containerless processing provides a high purity environment for the study of high-temperature, very reactive materials. It is an important method which provides access to the metastable state of an undercooled melt. In the absence of container walls, the nucleation rate is greatly reduced and undercooling up to (Tm-Tn)/Tm approx. 0.2 can be obtained, where Tm and Tn are the melting and nucleation temperatures, respectively. Electromagnetic levitation represents a method particularly well-suited for the study of metallic melts. The TEMPUS facility is a research instrument designed to perform electromagnetic levitation studies in reduced gravity. It provides temperatures up to 2600 C, levitation of several grams of material and access to the undercooled state for an extended period of time (up to hours).
Roger, Jan R.↗
LIRA: Probing the Lunar Surface for Resources.
The thermal, compositional and electrical properties of the lunar regolith, such as the triboelectric properties of lunar dust, the volatile content and the thermophysical properties of the polar regolith are essential to understand from a scientific perspective as well as for resource extraction and hazard mitigation. Understanding these properties specifically requires in situ measurement, as these properties are inherent in the ambient environment. With the Lunar In situ Regolith Analyzer (LIRA) we will combine mature surface sensor technologies from previous spacecraft missions (TECP) with new sensors (ClO4- ) and battery capability to achieve a compact in situ analysis package. The surface instrument suite is adapted for lunar conditions from a sensor developed as part of the Microscopy, Electrochemistry, and Conductivity Analyzer (MECA) instrument on the 2007 Mars Phoenix mission, as well as supplemental sensors. • The Thermal and Electrical Conductivity Probe (TECP), flown on MECA/Phoenix, which measures heat capacity, thermal conductivity, electrical resistance, and electrical permittivity between needles inserted into the soil. The TECP also measured atmospheric humidity, a capability that will be retained to search for subsurface H2O ice. The Mars TECP weighed 100-gram and by definition was at TRL 9. The lunar version (LIRA) is TRL 4. • The Perchlorate (ClO4) sensor is based on the fluorescence property of perchlorate (Mg, Fe, Na). We added a light source to excite the perchlorate (ClO4) and a photo diode to collect the emission wavelength. A sun-shade over the photo diode blocks out the ambient light during day light hours. The unifying goal of LIRA is to deliver a complete, integrated experiment suite that investigates properties that must be measured in situ, and provides the capability to power the suite at night or in PSRs, as well as a means to simulate aspects of the diurnal transition by delivering an optical pulse to the surface with intensity comparable to sunlight. We describe the scientific context of LIRA, touching on the electrical state of the near-surface environment, the thermal properties of the undisturbed regolith, and the distribution, geochemistry, and mobility of water. We also address the exploration implications of the work, examining hazards and resources in the context of strategic knowledge gaps (SKGs).
Heather Smith↗
New Hayabusa2 and OSIRIS REx Curation Facilities at Nasa Johnson Space Center
Asteroids are made up of rocky material that is left over from the formation of our solar system [1]. This material holds the key to unlocking information about the history of the planets and our sun. Hayabusa2 and OSIRIS-REx are two sample return missions that have collected rocky material from carbonaceous asteroids which are rich in water, carbon, and organic compounds [1]. Hayabusa2 is a spacecraft that collected samples from asteroid Ryugu launched by the Japan Aerospace Exploration Agency (JAXA). Samples were returned to Earth in December 2020 and 10 percent of the collected material is currently housed in a state-of-the-art laboratory at NASA Johnson Space Center (JSC) dedicated to Ryugu material [1]. OSIRIS-REx is a spacecraft launched by NASA that collected samples from asteroid Bennu. The samples are on their journey to Earth and will arrive in September 2023. They will be stored in a separate cleanroom dedicated to Bennu material. From these two missions, scientists will examine dust particles and rocky material to research clues on how the early solar system formed and how life began [2]. Because these samples are so precious and rare, it is important to keep them free from Earth contamination. In September of 2020, a massive construction project began to provide curation facilities to meet the needs of two incoming asteroid collections, Hayabusa2 and OSIRIS-REx. Despite numerous setbacks caused by the coronavirus pandemic, these facilities have been completed in the fall of 2021. Some of these setbacks included strict PPE requirements, keeping safe working distances as well as access to the facilities as NASA was operating under a Stage 3 condition in response to the pandemic. These facilities will provide curation laboratories to safely house the precious asteroid samples as well as facilities to manipulate samples, do sample preparation and process samples into and out of the labs for future studies by the scientific community. The lab suite consists of several different rooms including an initial anteroom (ISO 7), a staging area (ISO 7), two gowning areas (one for OSIRIS-REx and one for Hayabusa2; both ISO 6)), the Haybusa2 Curation Laboratory (ISO 5), the OSIRIS-REx Curation Laboratory (ISO 5), an ultramicrotomy laboratory (ISO 7) and a thin section laboratory (not a cleanroom). To maintain cleanliness levels necessary for these lab spaces quarterly to monthly Balazs organic and inorganic sampling has been conducted on a regular basis before, during and after the construction project to monitor the cleanliness levels in the new labs that will house the asteroid samples. These new facilities feature a Ruuvi (Grafana) monitoring system that measures temperature, humidity, and pressure of the lab suite. This is a small puck shaped sensor that sends signals to the Grafana cloud where the data can be plotted and set up on dashboards within the lab suite. These sensors are easy to set up and install and they run off a cell battery that is good for 3-5 years before needing replacement. This system can deliver text messages and emails to the appropriate personnel when values fall outside of set limits. In addition to the monitoring system, this new lab suite features viewing windows from several different locations and sliding motion censored doors. We have obtained some state-of-the-art equipment to outfit the lab spaces (desiccators, microscopes, ultramicrotome) and are in the process of outfitting the suite with additional items such as: nitrogen gloveboxes, additional desiccators, micromanipulators, etc. The Hayabusa2 samples provided to NASA by JAXA included 23 millimeter sized grains and 4 containers with finer material. OSIRIS-REx is expected to return as much as 400 grams of asteroid Bennu. Samples will be stored in a nitrogen glove box and separated into nitrogen sealed transfer containers. Small Bennu and Ryugu particles will be handled and prepared (e.g., ultra-thin sections for scanning electron microscopy (SEM) or transmission electron microscopy (TEM)) using an Axis Pro micromanipulator and Leica EM UC7 ultramicrotome. Analyzing samples at the atomic scale will provide greater insight to the origins of the solar system and life formation on Earth. All the curation preparation for these sample collections is essential to ongoing research and efforts to understand our solar system, both nowand for future generations.
Curation↗
Enhancing the Uranus PlanetGRAM with 2D Zonally-Averaged Atmospheric Variabilities
We developed an open-source Python package (tweModel.py1) that generates the 2D zonally-averaged atmospheric structure of Jupiter, Saturn, Uranus, and Neptune to be used as reference bases for NASA’s Planetary Global Reference Atmospheric Model (PlanetGRAM) Suite. The package outputs temperatures, pressures, densities, and zonal winds as functions of altitude and latitude given an input cloud-top zonal wind profile and a zonally averaged temperature map using a discretized form of the geostrophic thermal wind equation (TWE). We present 2D atmospheric structure outputs for Uranus in detail. Our results will be incorporated in the PlanetGRAM Suite to aid in the development of future in-situ missions in the outer solar system including the recently prioritized Uranus Flagship mission.
Uranus↗
Detecting Organic Compounds Released from Iron Oxidizing Bacteria using Sample Analysis at Mars (SAM) Like Instrument Protocols
Mars is a planet of great interest for Astrobiology since its past environmental conditions are thought to have been favourable for the emergence life. At present, the Red Planet is extremely cold and dry and the surface is exposed to intense UV and ionizing radiation, conditions generally considered to be incompatible with life as we know it on Earth. It was proposed that the shallow subsurface of Mars, where temperatures can be above freezing and liquid water can exist on rock surfaces, could harbor chemolithoautotrophic bacteria such as the iron oxidizing microorganism Pseudomonas sp. HerB. The Mars Science Laboratory (MSL) mission will provide the next opportunity to carry out in situ measurements for organic compounds of possible biological origin on Mars. One instrument onboard MSL, called the Sample Analysis at Mars (SAM) instrument suite, will carry out a broad and sensitive search for organic compounds in surface samples using either high temperature pyrolysis or chemical extraction followed by gas chromatography mass spectrometry. We present gas chromatograph mass spectrometer (GC/MS) data on crushed olivine rock powders that have been inoculated with Pseudomonas sp. HerB at different concentrations ranging from approx 10(exp 2) to 10(exp 7) cells per gram. The inoculated olivine samples were heated under helium carrier gas flow at 500 C and the pyrolysis products concentrated using a SAM-like hydrocarbon trap set at -20 C followed by trap heating and analysis by GC/Ms. In addition, the samples were also extracted using a low temperature "one-pot" chemical extraction technique using N-methyl, N-(tert-butyldimethylsilyl) trifluoroacetamide (MTBSTFA) as the silylating agent prior to GC/MS analysis. We identified several aldehydes, thiols, and alkene nitriles after pyrolysis GC/MS analysis of the bacteria that were not found in the olivine control samples that had not been inoculated with bacteria. The distribution of pyrolysis products extracted from the bacteria was clearly distinct from similar GC/MS analyses of the carbonaceous meteorite Murchison that was dominated by sulfur containing aromatic compounds. A similar comparison, if organic compounds are detected by SAM on Mars, could be useful to help discriminate between meteoritic or biological origins.
Glavin, D. P.↗
D/H ratios and H2O contents of mantle-derived amphibole megacrysts from Dish Hill, California
D/H ratios are, in principle, useful in characterizing reservoirs of mantle hydrogen and as tracers of volatile transfer processes in Earth's interior. In practice, however, interpretation of isotopic measurements on mantle derived H is complicated by surface processes such as contamination and degassing which may alter the primary D/H ratio. Although there are indications that water associated with subduction zones and certain chemically enriched basalts is enriched in D relative to 'typical' upper mantle water, the extent of isotopic heterogeneity of mantle H remains uncertain. Kaersutitic amphibole megacrysts in alkaline basalts are one of the most widespread sources of mantle water and are therefore potentially useful for large-scale regional studies of D/H variation. However, D/H ratios of these amphiboles vary widely (from plus 8 to minus 113 percent), even in samples from the same locality, so that this potential has yet to be realized. In order to investigate the origin of this variability, and to explore the possibility that primary mantle D/H ratios may be deduced from these amphiboles, we analyzed the D/H ratios and chemical compositions of a suite of 17 kaersutitic amphiboles from Dish Hill, California. This work contrasts with previous studies in which sampling is widespread, but representatives from any given locality are few. Samples were collected from a restricted area on the southern flank of the volcanic center and are associated with the basal volcanic breccia. Fourteen of the samples were large single crystals or crystal fragments (megacrysts, 0.4 to 30 grams), believed to derive from pegmatitic veins crystallized from melts in the mantle. Two were coarse-grained intergrowths of amphibole with olivine and spinel, and one was a thin (2 mm) selvage on a peridotite xenolith.
Bell, David R.↗
Northwest Africa 1401: A Polymict Cumulate Eucrite with a Unique Ferroan Heteradcumulate Mafic Clast
The howardite, eucrite and diogenite (HED) clan is the largest suite of achondrites available for study. The suite gives us a unique view of the magmatism that affected some asteroids early in solar system history. One problem with mining the HED clan for petrogenetic information is that there is only limited petrologic diversity among the rock types. Thus, discovering unusual HED materials holds the potential for revealing new insights into the petrologic evolution of the HED parent asteroid. Here we report on petrologic study of an unusual, 27 gram polymict eucrite, Northwest Africa (NWA) 1401. The thin section studied (approx. 20 x 10 mm) contains one large, ferroan clast described separately. The remainder of the rock, including mineral fragments and other, smaller lithic clasts, forms the host breccia.
Mittlefehldt, David W.↗
Enhanced Microbial Detection Capabilities by a Rapid Portable Instrument
We present data describing a progression of continuing technology development - from expanding the detection capabilities of the current PTS unit to re-outfitting the instrument with a protein microarray increasing the number of detectable compounds. To illustrate the adaptability of the cartridge format, on-orbit operations data from the ISS demonstrate the detection of the fungal cell wall compound beta-glucan using applicable LOCAD-PTS cartridges. LOCAD-PTS is a handheld device consisting of a spectrophotometer, an onboard pumping mechanism, and data storage capabilities. A suite of interchangeable cartridges lined with four distinct capillaries allow a hydrated sample to mix with necessary reagents in the channels before being pumped to the optical well for spectrophotometric analysis. The reagents housed in one type of cartridge trigger a reaction based on the Limulus Amebocyte Lysate (LAL) assay, which results in the release of paranitroaniline dye. The dye is measured using a 395 nm filter. The LAL assay detects the Gram-negative bacterial cell wall molecule, endotoxin or lipopolysaccharide (LPS). The more dye released, the greater the concentration of endotoxin in the sample. Sampling, quantitative analysis, and data retrieval require less than 20 minutes. This is significantly faster than standard culture-based methods, which require at least a 24 hour incubation period.Using modified cartridges, we demonstrate the detection of Gram negative bacteria with protein microarray technology. Additionally, we provide data from multiple field tests where both standard and advanced PTS technologies were used. These tests investigate the transfer of target microbial molecules from one surface to another. Collectively, these data demonstrate that the new cartridges expand the number of compounds detected by LOCAD-PTS, while maintaining the rapid, in situ analysis characteristic of the instrument. The unit provides relevant data for verifying sterile sample collection protocols, which are critical for conducting accurate scientific experiments during future missions to the Moon and Mars.
Morris, Heather↗