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At least 55 records · Page 3

Iris Deep-Space Transponder for SLS EM-1 CubeSat Missions

Several key design upgrades have been recently made to the Iris Deep-Space Transponder baselined to be used on secondary payload missions of the upcoming Space Launch System Exploration Mission One (SLS EM-1). The Iris Transponder is a reconfigurable software-defined radio (SDR) designed for missions requiring interoperability with NASA's Deep Space Network (DSN) on X-band frequencies (7.2 GHz uplink, 8.4 GHz downlink). The transponder provides radiometric tracking support with the DSN to provide navigational products for precise orbit determination while performing standard uplink and downlink communications in a CubeSat/SmallSat-applicable package size. The recent hardware changes include a volume reduction of 30% from previous models, with the main transponder unit weighing less than 1.0 kg, while maintaining total ionizing dose (TID) radiation tolerance of >23 krads (Si) for deep-space missions. Lab tests indicate maximum downlink rates of up to 6.25 Msps can be supported by the transponder in conventional BPSK modulation, but most SLS EM-1 CubeSat missions are link-performance limited to less than 256 kbps. A prototype unit of the flight production units has been assembled and various performance and characterization tests are underway. This talk discusses the key design aspects and specifications of the Iris Transponder and presents preliminary results from early testing of the assembled transponder.

Kobayashi, M. Michael

Cryo-EM Structure of the Mnx Protein Complex Reveals a Tunnel Framework for the Mechanism of Manganese Biomineralization

The global manganese cycle relies on microbes to oxidize soluble Mn(II) to insoluble Mn(IV) oxides. Some microbes require peroxide or superoxide as oxidants, but others can use O 2 directly, via multicopper oxidase (MCO) enzymes. One of these, MnxG from Bacillus sp. strain PL-12, was isolated in tight association with small accessory proteins, MnxE and MnxF. The protein complex, called Mnx, has eluded crystallization efforts, but we now report the 3D structure of a point mutant using cryo-EM single particle analysis, cross-linking mass spectrometry, and AlphaFold Multimer prediction. The ß-sheet–rich complex features MnxG enzyme, capped by a heterohexameric ring of alternating MnxE and MnxF subunits, and a tunnel that runs through MnxG and its MnxE 3 F 3 cap. The tunnel dimensions and charges can accommodate the mechanistically inferred binuclear manganese intermediates. Furthermore, comparison with the Fe(II)-oxidizing MCO, ceruloplasmin, identifies likely coordinating groups for the Mn(II) substrate, at the entrance to the tunnel. Thus, the 3D structure provides a rationale for the established manganese oxidase mechanism, and a platform for further experiments to elucidate mechanistic details of manganese biomineralization.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH

Naturally ornate RNA-only complexes revealed by cryo-EM

The structures of natural RNAs remain poorly characterized and may hold numerous surprises. Here we report three-dimensional structures of three large ornate bacterial RNAs using cryo-electron microscopy (cryo-EM). GOLLD (Giant, Ornate, Lake- and Lactobacillales-Derived), ROOL (Rumen-Originating, Ornate, Large) and OLE (Ornate Large Extremophilic) RNAs form homo-oligomeric complexes whose stoichiometries are retained at lower concentrations than measured in cells. OLE RNA forms a dimeric complex with long co-axial pipes spanning two monomers. Both GOLLD and ROOL form distinct RNA-only multimeric nanocages with diameters larger than the ribosome, each empty except for a disordered loop. Extensive intramolecular and intermolecular A-minor interactions, kissing loops, an unusual A–A helix and other interactions stabilize the three complexes. Sequence covariation analysis of these large RNAs reveals evolutionary conservation of intermolecular interactions, supporting the biological importance of large, ornate RNA quaternary structures that can assemble without any involvement of proteins.

59 BASIC BIOLOGICAL SCIENCES

Cryo-EM structures of DNA-free and DNA-bound BsaXI: architecture of a Type IIB restriction–modification enzyme

Abstract We have determined multiple cryogenic electron microscopy (cryo-EM) structures of the Type IIB restriction–modification enzyme BsaXI. Such enzymes cleave DNA on both sides of their recognition sequence and share features of Types I, II, and III restriction systems. BsaXI forms a heterotrimeric (RM)2S assemblage in the presence and absence of bound DNA. Two unique structural motifs—a multi-helical “knob” and a long antiparallel double-helical “paddle”—are involved in DNA binding and cleavage. Binding of the DNA target triggers a large conformational change from an ‘open’ to ‘closed’ configuration, resulting in a mixture of two different conformations with respect to the positioning of the S subunit and its target recognition domains on the enzyme’s bipartite DNA target site. Structure-guided mutagenesis studies implicated two clusters of residues in the RM subunit as being critical for DNA cleavage, both are located proximal to a DNA cleavage site. One corresponds to a canonical PD-(D/E)xK endonuclease site in the N-terminal endonuclease domain, while the other corresponds to residues clustered within the paddle motif (near to the C-terminal end of the RM subunit). This analysis facilitates a comparison of three potential mechanisms by which such enzymes cleave DNA on each side of the bound target.

Shen, Betty W.

Cryo-EM structure of a photosystem I variant containing an unusual plastoquinone derivative in its electron transfer chain

Photosystem I (PS I) is a light-driven oxidoreductase responsible for converting photons into chemical bond energy. Its application for renewable energy was revolutionized by the creation of the MenB deletion (ΔmenB) variant in the cyanobacterium Synechocystis sp. PCC 6803, in which phylloquinone is replaced by plastoquinone-9 with a low binding affinity. This permits its exchange with exogenous quinones covalently coupled to dihydrogen catalysts that bind with high affinity, thereby converting PS I into a stable solar fuel catalyst. Here, we reveal the 2.03-Å-resolution cryo-EM structure of a recent MenB variant of PS I. The quinones and their binding environment are analyzed in the context of previous biophysical data, thereby enabling a protocol to solve future PS I hybrids and constructs from this genetically tractable cyanobacterium.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH

Trapping and imaging dynamic battery nanointerfaces via electrified cryo-EM

The electrified interface between a liquid and a solid underpins diverse phenomena, from ion-transfer during battery operation to action potentials enabling biological communication. However, conventional tools are blind to the nanoscale dynamics of this metastable interface. Here, we leverage electrified cryo–electron microscopy (eCryo-EM), a technique that rapidly freezes and kinetically traps these dynamic, nonequilibrium states during battery operation for nanoscale characterization. Collective snapshots of the electrified interface at controlled time intervals quantifies early-stage growth kinetics of the solid electrolyte interphase (SEI), a passivation film that governs electron and ion transport. Unexpectedly, the diffusivity of charged species of the two SEI films with differing chemistry and performance are estimated to be within 10% of the other, indicated by the slope of their diffusion-limited SEI growth regimes. Instead, the slope of the reaction-limited SEI growth regimes differs by a factor of 3, suggesting that lowered reactivity of the high-performance electrolyte is largely responsible for its high coulombic efficiency.

Science & Technology - Other Topics

Gateway Cities Unplugged: (em)Powering Affordable Housing (Final Technical Report)

The Gateway Cities Unplugged: (em)Powering Affordable Housing (the “Project”) was launched to address a critical challenge in the U.S. building and energy sectors: enabling multifamily buildings especially those serving low and moderate‑income (LMI) communities to actively support grid modernization through Grid‑Interactive Efficient Buildings (GEB). Multifamily housing represents a large share of national building energy consumption, yet the sector faces persistent barriers to adopting advanced load flexibility technologies, including capital constraints, aging infrastructure, and limited access to demand‑side management tools. This Project sought to overcome those barriers by defining, evaluating, and designing commercially viable GEB technology packages for six representative multifamily properties across Massachusetts, Connecticut, and New York.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI

Observations of the rapid oscillations of EM Cygni

High-speed two-color photometric observations have been made of the dwarf nova EM Cyg and rapid coherent oscillations with periods between 14.6 and 16.5 sec have been found. The minimum period is less than the period of a Keplerian orbit at the surface of a carbon white dwarf. The color temperature of the oscillating component is much higher than that of the constant component of the luminosity. These results suggest that the source of the oscillations is the white dwarf or the inner part of the accretion disk.

Stiening, R. F.

A uniform GTD analysis of the EM diffraction by a thin dielectric/ferrite half-plane and related configurations

A uniform geometrical theory of diffraction (UTD) solution is developed for the problem of the diffraction by a thin dielectric/ferrite half plane when it is excited by a plane, cylindrical, or surface wave field. Both transverse electric and transverse magnetic cases are considered. The solution of this problem is synthesized from the solutions to the related problems of EM diffraction by configurations involving perfectly conducting electric and magnetic walls covered by a dielectric/ferrite half-plane of one half the thickness of the original half-plane.

Rojas, Roberto G.

Analysis of the EM scattering from arbitrary open-ended waveguide cavities using axial Gaussian Beam tracking

The electromagnetic (EM) scattering from a planar termination located inside relatively arbitrarily shaped open-ended waveguide cavities with smoothly curved interior walls is analyzed using a Gaussian Beam (GB) expansion of the incident plane wave fields in the open end. The cavities under consideration may contain perfectly-conducting interior walls with or without a thin layer of material coating, or the walls may be characterized by an impedance boundary condition. In the present approach, the GB's are tracked only to the termination of the waveguide cavity via beam reflections from interior waveguide cavity walls. The Gaussian beams are tracked approximately only along their beam axes; this approximation which remains valid for relatively well focussed beams assumes that an incident GB gives rise to a reflected GB with parameters related to the incident beam and the radius of curvature of the wall. It is found that this approximation breaks down for GB's which come close to grazing a convex surface and when the width of the incident beam is comparable to the radius of curvature of the surface. The expansion of the fields at the open end depend on the incidence angle only through the expansion coefficients, so the GB's need to be tracked through the waveguide cavity only once for a wide range of incidence angles. At the termination, the sum of all the GB's are integrated using a result developed from a generalized reciprocity principle, to give the fields scattered from the interior of the cavity. The rim edge at the open end of the cavity is assumed to be sharp and the external scattering from the rim is added separately using Geometrical Theory of Diffraction. The results based on the present approach are compared with solutions based on the hybrid asymptotic modal method. The agreement is found to be very good for cavities made up of planar surfaces, and also for cavities with curved surfaces which are not too long with respect to their width.

Burkholder, R. J.

A hybrid asymptotic-modal analysis of the EM scattering by an open-ended S-shaped rectangular waveguide cavity

The electromagnetic fields (EM) backscatter from a 3-dimensional perfectly conducting S-shaped open-ended cavity with a planar interior termination is analyzed when it is illuminated by an external plane wave. The analysis is based on a self-consistent multiple scattering method which accounts for the multiple wave interactions between the open end and the interior termination. The scattering matrices which described the reflection and transmission coefficients of the waveguide modes reflected and transmitted at each junction between the different waveguide sections, as well at the scattering from the edges at the open end are found via asymptotic high frequency methods such as the geometrical and physical theories of diffraction used in conjunction with the equivalent current method. The numerical results for an S-shaped inlet cavity are compared with the backscatter from a straight inlet cavity; the backscattered patterns are different because the curvature of an S-shaped inlet cavity redistributes the energy reflected from the interior termination in a way that is different from a straight inlet cavity.

Law, P. H.

Modal, ray, and beam techniques for analyzing the EM scattering by open-ended waveguide cavities

The problem of high-frequency electromagnetic (EM) scattering by open-ended waveguide cavities with an interior termination is analyzed via three different approaches. When cavities can be adequately modeled by joining together piecewise separable waveguide sections, a hybrid combination of asymptotic high-frequency and modal techniques is employed. In the case of more arbitrarily shaped waveguide cavities for which modes cannot even be defined in the conventional sense, the geometrical optics ray approach proves to be highly useful. However, at sufficiently high frequencies, both of these approaches tend to become inefficient. Hence, a paraxial Gaussian batch technique, which retains much of the simplicity of the ray approximation but is potentially more efficient, is investigated. Typical numerical results based on the different approaches are discussed.

Pathak, Prabhakar H.

High-frequency asymptotic methods for analyzing the EM scattering by open-ended waveguide cavities

Four high-frequency methods are described for analyzing the electromagnetic (EM) scattering by electrically large open-ended cavities. They are: (1) a hybrid combination of waveguide modal analysis and high-frequency asymptotics, (2) geometrical optics (GO) ray shooting, (3) Gaussian beam (GB) shooting, and (4) the generalized ray expansion (GRE) method. The hybrid modal method gives very accurate results but is limited to cavities which are made up of sections of uniform waveguides for which the modal fields are known. The GO ray shooting method can be applied to much more arbitrary cavity geometries and can handle absorber treated interior walls, but it generally only predicts the major trends of the RCS pattern and not the details. Also, a very large number of rays need to be tracked for each new incidence angle. Like the GO ray shooting method, the GB shooting method can handle more arbitrary cavities, but it is much more efficient and generally more accurate than the GO method because it includes the fields diffracted by the rim at the open end which enter the cavity. However, due to beam divergence effects the GB method is limited to cavities which are not very long compared to their width. The GRE method overcomes the length-to-width limitation of the GB method by replacing the GB's with GO ray tubes which are launched in the same manner as the GB's to include the interior rim diffracted field. This method gives good accuracy and is generally more efficient than the GO method, but a large number of ray tubes needs to be tracked.

Burkholder, R. J.

Testing For EM Upsets In Aircraft Control Computers

Effects of transient electrical signals evaluated in laboratory tests. Method of evaluating nominally fault-tolerant, aircraft-type digital-computer-based control system devised. Provides for evaluation of susceptibility of system to upset and evaluation of integrity of control when system subjected to transient electrical signals like those induced by electromagnetic (EM) source, in this case lightning. Beyond aerospace applications, fault-tolerant control systems becoming more wide-spread in industry; such as in automobiles. Method supports practical, systematic tests for evaluation of designs of fault-tolerant control systems.

Belcastro, Celeste M.

Solar wind-magnetosphere interaction as simulated by a 3D, EM particle code

The results of simulating the solar wind-magnetosphere interaction with a three dimensional, electromagnetic (EM) particle code are presented. Hitherto such global simulations were done with magnetohydrodynamic (MHD) codes while lower dimensional particle or hybrid codes served to account for microscopic processes and such transport parameters as have to be introduced ad hoc in MHD. The kinetic model combines macroscopic and microscopic tasks. It relies only on the Maxwell curl equations and the Lorentz equation for particles. The preliminary results are for an unmagnetized solar wind plasma streaming past a dipolar magnetic field. The results show the formation of a bow shock and a magnetotail, the penetration of energetic particles into cusp and radiation belt regions, and dawn to dusk asymmetries.

Buneman, O.

The European Mobile System (EMS)

The European Space Agency is presently procuring an L band payload in order to promote a regional European L band system coping with the specific needs of the European market. The payload, and the two communications systems to be supported, are described below. The potential market for EMS in Europe is discussed.

Jongejans, A.

A uniform geometrical optics and an extended uniform geometrical theory of diffraction for evaluating high frequency EM fields near smooth caustics and composite shadow boundaries

A uniform geometrical optics (UGO) and an extended uniform geometrical theory of diffraction (EUTD) are developed for evaluating high frequency electromagnetic (EM) fields within transition regions associated with a two and three dimensional smooth caustic of reflected rays and a composite shadow boundary formed by the caustic termination or the confluence of the caustic with the reflection shadow boundary (RSB). The UGO is a uniform version of the classic geometrical optics (GO). It retains the simple ray optical expressions of classic GO and employs a new set of uniform reflection coefficients. The UGO also includes a uniform version of the complex GO ray field that exists on the dark side of the smooth caustic. The EUTD is an extension of the classic uniform geometrical theory of diffraction (UTD) and accounts for the non-ray optical behavior of the UGO reflected field near caustics by using a two-variable transition function in the expressions for the edge diffraction coefficients. It also uniformly recovers the classic UTD behavior of the edge diffracted field outside the composite shadow boundary transition region. The approach employed for constructing the UGO/EUTD solution is based on a spatial domain physical optics (PO) radiation integral representation for the fields which is then reduced using uniform asymptotic procedures. The UGO/EUTD analysis is also employed to investigate the far-zone RCS problem of plane wave scattering from two and three dimensional polynomial defined surfaces, and uniform reflection, zero-curvature, and edge diffraction coefficients are derived. Numerical results for the scattering and diffraction from cubic and fourth order polynomial strips are also shown and the UGO/EUTD solution is validated by comparison to an independent moment method (MM) solution. The UGO/EUTD solution is also compared with the classic GO/UTD solution. The failure of the classic techniques near caustics and composite shadow boundaries is clearly demonstrated and it is shown that the UGO/EUTD results remain valid and uniformly reduce to the classic results away from the transition regions. Mathematical details on the asymptotic properties and efficient numerical evaluation of the canonical functions involved in the UGO/EUTD expressions are also provided.

Constantinides, E. D.