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At least 55 records · Page 3

X-ray bright points and high-speed wind streams: A preliminary analysis from Yohkoh and Ulysses data

The following aspect of the solar wind mass flux, and of its variation, is examined: whether coronal plumes might be responsible for the long-term variability of the mass flux in high-speed streams emanating from coronal holes. The assumption that plumes are rooted in coronal bright points (BP's) is made. The behavior of X-ray BP's, imaged by the Yohkoh soft X-ray telescope (SXT), during a seven month period when Ulysses experiments observed a series of recurrent high-speed streams, is analyzed. If plumes/BP's are sources of the wind mass flux, changes in the coronal hole BP density to mimic changes of the mass flux in high-speed streams are expected. SOHO will have the capability of measuring the solar wind speed/density at small heliocentric distances while simultaneously observing coronal BP's and coronal plumes.

Poletto, Giannina↗

Calcium metabolism and cardiovascular function after spaceflight

To determine the influence of dietary calcium on spaceflight-induced alterations in calcium metabolism and blood pressure (BP), 9-wk-old spontaneously hypertensive rats, fed either high- (2%) or low-calcium (0.02%) diets, were flown on an 18-day shuttle flight. On landing, flight animals had increased ionized calcium (P < 0.001), elevated parathyroid hormone levels (P < 0.001), reduced calcitonin levels (P < 0.05), unchanged 1,25(OH)(2)D(3) levels, and elevated skull (P < 0.01) and reduced femur bone mineral density. Basal and thrombin-stimulated platelet free calcium (intracellular calcium concentration) were also reduced (P < 0.05). There was a tendency for indirect systolic BP to be reduced in conscious flight animals (P = 0.057). However, mean arterial pressure was elevated (P < 0.001) after anesthesia. Dietary calcium altered all aspects of calcium metabolism (P < 0.001), as well as BP (P < 0.001), but the only interaction with flight was a relatively greater increase in ionized calcium in flight animals fed low- compared with high-calcium diets (P < 0.05). The results indicate that 1) flight-induced disruptions of calcium metabolism are relatively impervious to dietary calcium in the short term, 2) increased ionized calcium did not normalize low-calcium-induced elevations of BP, and 3) parathyroid hormone was paradoxically increased in the high-calcium-fed flight animals after landing.

NASA Discipline Cardiopulmonary↗

Radiocarbon ages of lacustrine deposits in volcanic sequences of the Lomas Coloradas area, Socorro Island, Mexico

Extensive eruptions of alkalic basalt from low-elevation fissures and vents on the southern flank of the dormant volcano, Cerro Evermann, accompanied the most recent phase of volcanic activity on Socorro Island, and created the Lomas Coloradas, a broad, gently sloping terrain comprising the southern part of the island. We obtained 14C ages of 4690 +/- 270 BP (5000-5700 cal BP) and 5040 +/- 460 BP (5300-6300 cal BP) from lacustrine deposits that occur within volcanic sequences of the lower Lomas Coloradas. Apparently, the sediments accumulated within a topographic depression between two scoria cones shortly after they formed. The lacrustine environment was destroyed when the cones were breached by headward erosion of adjacent stream drainages. This was followed by the eruption of a thin basaltic flow from fissures near the base of the northernmost cone. The flow moved downslope for a short distance and into the drainages that presently bound the study area on the east and west. The flow postdates development of the present drainage system and may be very recent. Our 14C data, along with historical accounts of volcanic activity over the last century, including submarine eruptions that occurred a few km west of Socorro in early 1993, underscore the high risk for explosive volcanism in this region and the need for a detailed volcanic hazards plan and seismic monitoring.

NASA Center ARC↗

In vivo regulation of the beta-myosin heavy chain gene in soleus muscle of suspended and weight-bearing rats

In the weight-bearing hindlimb soleus muscle of the rat, approximately 90% of muscle fibers express the beta-myosin heavy chain (beta-MHC) isoform protein. Hindlimb suspension (HS) causes the MHC isoform population to shift from beta toward the fast MHC isoforms. Our aim was to establish a model to test the hypothesis that this shift in expression is transcriptionally regulated through specific cis elements of the beta-MHC promoter. With the use of a direct gene transfer approach, we determined the activity of different length beta-MHC promoter fragments, linked to a firefly luciferase reporter gene, in soleus muscle of control and HS rats. In weight-bearing rats, the relative luciferase activity of the longest beta-promoter fragment (-3500 bp) was threefold higher than the shorter promoter constructs, which suggests that an enhancer sequence is present in the upstream promoter region. After 1 wk of HS, the reporter activities of the -3500-, -914-, and -408-bp promoter constructs were significantly reduced ( approximately 40%), compared with the control muscles. However, using the -215-bp construct, no differences in promoter activity were observed between HS and control muscles, which indicates that the response to HS in the rodent appears to be regulated within the -408 and -215 bp of the promoter.

Non-NASA Center↗

Long-range correlation properties of coding and noncoding DNA sequences: GenBank analysis

An open question in computational molecular biology is whether long-range correlations are present in both coding and noncoding DNA or only in the latter. To answer this question, we consider all 33301 coding and all 29453 noncoding eukaryotic sequences--each of length larger than 512 base pairs (bp)--in the present release of the GenBank to dtermine whether there is any statistically significant distinction in their long-range correlation properties. Standard fast Fourier transform (FFT) analysis indicates that coding sequences have practically no correlations in the range from 10 bp to 100 bp (spectral exponent beta=0.00 +/- 0.04, where the uncertainty is two standard deviations). In contrast, for noncoding sequences, the average value of the spectral exponent beta is positive (0.16 +/- 0.05) which unambiguously shows the presence of long-range correlations. We also separately analyze the 874 coding and the 1157 noncoding sequences that have more than 4096 bp and find a larger region of power-law behavior. We calculate the probability that these two data sets (coding and noncoding) were drawn from the same distribution and we find that it is less than 10(-10). We obtain independent confirmation of these findings using the method of detrended fluctuation analysis (DFA), which is designed to treat sequences with statistical heterogeneity, such as DNA's known mosaic structure ("patchiness") arising from the nonstationarity of nucleotide concentration. The near-perfect agreement between the two independent analysis methods, FFT and DFA, increases the confidence in the reliability of our conclusion.

Non-NASA Center↗

Contrasting effects of vasodilators on blood pressure and sodium balance in the hypertension of autonomic failure

Supine hypertension, which is very common in patients with autonomic failure, limits the use of pressor agents and induces nighttime natriuresis. In 13 patients with severe orthostatic hypotension due to autonomic failure (7 women, 6 men, 72 +/- 3 yr) and supine hypertension, the effect of 30 mg nifedipine (n = 10) and 0.025 to 0.2 mg/h nitroglycerin patch (n = 11) on supine BP, renal sodium handling, and orthostatic tolerance was determined. Medications were given at 8 p.m.; patients stood up at 8 a.m. Nitroglycerin was removed at 6 a.m. Compared with placebo, nifedipine and nitroglycerin decreased systolic BP during the night by a maximum of 37 +/- 9 and 36 +/- 10 mmHg, respectively (P < 0.01). At 8 a.m., supine systolic BP was 23 +/- 7 mmHg lower with nifedipine than with placebo (P < 0.05), but was similar with nitroglycerin and placebo. Sodium excretion during the night was not reduced with nitroglycerin (0.13 +/- 0.02 mmol/mg creatinine [Cr] versus 0.15 +/- 0.03 mmol/mg Cr with placebo), but it was increased with nifedipine (0.35 +/- 0.06 mmol/mg Cr versus 0.13 +/- 0.02 mmol/mg Cr with placebo, P < 0.05). Nifedipine but not nitroglycerin worsened orthostatic hypotension in the morning. It is concluded that nifedipine and transdermal nitroglycerin are effective in controlling supine hypertension in patients with autonomic failure. However, nifedipine has a prolonged depressor effect and worsens orthostatic hypotension in the morning. The decrease in pressure natriuresis that would be expected with the substantial decrease in BP obtained with nitroglycerin and nifedipine may be offset by a direct effect of both drugs on renal sodium handling.

Clinical Trial↗

Chronic orthostatic intolerance: a disorder with discordant cardiac and vascular sympathetic control

BACKGROUND: Chronic orthostatic intolerance (COI) is a debilitating autonomic condition in young adults. Its neurohumoral and hemodynamic profiles suggest possible alterations of postural sympathetic function and of baroreflex control of heart rate (HR). METHODS AND RESULTS: In 16 COI patients and 16 healthy volunteers, intra-arterial blood pressure (BP), ECG, central venous pressure (CVP), and muscle sympathetic nerve activity (MSNA) were recorded at rest and during 75 degrees tilt. Spectral analysis of RR interval and systolic arterial pressure (SAP) variabilities provided indices of sympathovagal modulation of the sinoatrial node (ratio of low-frequency to high-frequency components, LF/HF) and of sympathetic vasomotor control (LFSAP). Baroreflex mechanisms were assessed (1) by the slope of the regression line obtained from changes of RR interval and MSNA evoked by pharmacologically induced alterations in BP and (2) by the index alpha, obtained from cross-spectral analysis of RR and SAP variabilities. At rest, HR, MSNA, LF/HF, and LFSAP were higher in COI patients, whereas BP and CVP were similar in the two groups. During tilt, BP did not change and CVP fell by the same extent in the 2 groups; the increase of HR and LF/HF was more pronounced in COI patients. Conversely, the increase of MSNA was lower in COI than in control subjects. Baroreflex sensitivity was similar in COI and control subjects at rest; tilt reduced alpha similarly in both groups. CONCLUSIONS: COI is characterized by an overall enhancement of noradrenergic tone at rest and by a blunted postganglionic sympathetic response to standing, with a compensatory cardiac sympathetic overactivity. Baroreflex mechanisms maintain their functional responsiveness. These data suggest that in COI, the functional distribution of central sympathetic tone to the heart and vasculature is abnormal.

Non-NASA Center↗

1,25-Dihydroxyvitamin D3 inhibition of col1a1 promoter expression in calvariae from neonatal transgenic mice

We studied the effect of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) on organ cultures of transgenic mouse calvariae containing segments of the Col1a1 promoter extending to -3518, -2297, -1997, -1794, -1763, and -1719 bp upstream of the transcription start site fused to the chloramphenicol acetyltransferase (CAT) reporter gene. 1,25(OH)2D3 had a dose-dependent inhibitory effect on the expression of the -3518 bp promoter construct (ColCAT3.6), with maximal inhibition of about 50% at 10 nM. This level of inhibition was consistent with the previously observed effect on the endogenous Col1a1 gene in bone cell models. All of the shorter constructs were also inhibited by 10 nM 1,25(OH)2D3, suggesting that the sequences required for 1, 25(OH)2D3 inhibition are downstream of -1719 bp. The inhibitory effect of 1,25(OH)2D3 on transgene mRNA was maintained in the presence of the protein synthesis inhibitor cycloheximide, suggesting that the inhibitory effect on Col1a1 gene transcription does not require de novo protein synthesis. We also examined the in vivo effect of 1,25(OH)2D3 treatment of transgenic mice on ColCAT activity, and found that 48 h treatment caused a dose-dependent inhibition of CAT activity in calvariae comparable to that observed in organ cultures. In conclusion, we demonstrated that 1,25(OH)2D3 inhibits Col1A1 promoter activity in transgenic mouse calvariae, both in vivo and in vitro. The results indicate that there is a 1, 25(OH)2D3 responsive element downstream of -1719 bp. The inhibitory effect does not require new protein synthesis.

NASA Discipline Cell Biology↗

Structural characterization and regulatory element analysis of the heart isoform of cytochrome c oxidase VIa

In order to investigate the mechanism(s) governing the striated muscle-specific expression of cytochrome c oxidase VIaH we have characterized the murine gene and analyzed its transcriptional regulatory elements in skeletal myogenic cell lines. The gene is single copy, spans 689 base pairs (bp), and is comprised of three exons. The 5'-ends of transcripts from the gene are heterogeneous, but the most abundant transcript includes a 5'-untranslated region of 30 nucleotides. When fused to the luciferase reporter gene, the 3.5-kilobase 5'-flanking region of the gene directed the expression of the heterologous protein selectively in differentiated Sol8 cells and transgenic mice, recapitulating the pattern of expression of the endogenous gene. Deletion analysis identified a 300-bp fragment sufficient to direct the myotube-specific expression of luciferase in Sol8 cells. The region lacks an apparent TATA element, and sequence motifs predicted to bind NRF-1, NRF-2, ox-box, or PPAR factors known to regulate other nuclear genes encoding mitochondrial proteins are not evident. Mutational analysis, however, identified two cis-elements necessary for the high level expression of the reporter protein: a MEF2 consensus element at -90 to -81 bp and an E-box element at -147 to -142 bp. Additional E-box motifs at closely located positions were mutated without loss of transcriptional activity. The dependence of transcriptional activation of cytochrome c oxidase VIaH on cis-elements similar to those found in contractile protein genes suggests that the striated muscle-specific expression is coregulated by mechanisms that control the lineage-specific expression of several contractile and cytosolic proteins.

Non-NASA Center↗

2011 NASA Lunabotics Mining Competition for Universities: Results and Lessons Learned

Overview: Design, build & compete remote controlled robot (Lunabot). Excavate Black Point 1 (BP-1) Lunar Simulant. Deposit minimum of 10 kg of BP-1 within 15 minutes $5000, $2500, $1000 Scholarships for most BP-1 excavated. May 23-28, 2011. Kennedy Space Center, FL. International Teams Allowed for the First Time. What is a Lunabot? a) Robot Controlled Remotely or Autonomously. b) Visual and Auditory Isolation from Operator. c) Excavates Black Point 1 (BP-l) Simulant. d) Weight Limit - 80 kg. e)Dimension Limits -1.5m width x .75m length x 2m height. f) Designed, Built and Tested by University Student Teams.

Mueller, Robert P.↗

Femoral Blood Flow and Cardiac Output During Blood Flow Restricted Leg Press Exercise

Low load blood flow restricted resistance exercise (LBFR) causes muscle hypertrophy that may be stimulated by the local ischemic environment created by the cuff pressure. However, local blood flow (BF) during such exercise is not well understood. PURPOSE: To characterize femoral artery BF and cardiac output (CO) during leg press exercise (LP) performed at a high load (HL) and low load (LL) with different levels of cuff pressure. METHODS: Eleven subjects (men/women 4/7, age 31.4+/-12.8 y, weight 68.9+/-13.2 kg, mean+/-SD) performed 3 sets of supine left LP to fatigue with 90 s of rest in 4 conditions: HL (%1-RM/cuff pressure: 80%/0); LL (20%/0); LBFR(sub DBP) (20%/1.3 x diastolic blood pressure, BP); LBFR(sub SBP) (20%/1.3 x supine systolic BP). The cuff remained inflated throughout the LBFR exercise sessions. Artery diameter, velocity time integral (VTI), and stroke volume (SV) were measured using Doppler ultrasound at rest and immediately after each set of exercise. Heart rate (HR) was monitored using a 3-lead ECG. BF was calculated as VTI x vessel cross-sectional area. CO was calculated as HR x SV. The data obtained after each set of exercise were averaged and used for analyses. Multi-level modeling was used to determine the effect of exercise condition on dependent variables. Statistical significance was set a priori at p< 0.05. RESULTS: Artery diameter did not change from baseline. BF increased (p<0.05) after exercise in each condition except LBFR(sub SBP) in the order of HL (12.73+/-1.42 cm3,mean+/-SE) > LL (9.92+/-0.82 cm3) > LBFR(sub dBP)(6.47+/-0.79 cm3) > LBFR(sub SBP) (3.51+/-0.59 cm3). Blunted exercise induced increases occurred in HR, SV, and CO after LBFR compared to HL and LL. HR increased 45% after HL and LL and 28% after LBFR (p<0.05), but SV increased (p<0.05) only after HL. Consequently, the increase (p<0.05) in CO was greater in HL and LL (approximately 3 L/min) than in LBFR (approximately 1 L/min). CONCLUSION: BF during LBFR(sub SBP) was 1/3 of that observed in LL, which supports the hypothesis that local ischemia stimulates the LBFR hypertrophic response. As the cuff did not compress the artery, the ischemia may have occurred because of the blunted rise in CO or because arterial BP cannot overcome the cuff pressure. As LBFR(sub DBP) effectively reduced BF and CO with cuff pressures less than systolic BP, future studies should investigate the hypertrophic potential of LBFR at even lower cuff pressures.

Everett, M. E.↗

Absorption Cross-Sections of Ozone in the Ultraviolet and Visible Spectral Regions: Status report 2015

The activity Absorption Cross-Sections of Ozone (ACSO) started in 2008 as a joint initiative of the International Ozone Commission (IO3C), the World Meteorological Organization (WMO) and the IGACO (Integrated Global Atmospheric Chemistry Observations) O3/UV subgroup to study, evaluate, and recommend the most suitable ozone absorption cross-section laboratory data to be used in atmospheric ozone measurements. The evaluation was basically restricted to ozone absorption cross-sections in the UV range with particular focus on the Huggins band. Up until now, the data of Bass and Paur published in 1985 (BP, 1985) are still officially recommended for such measurements. During the last decade it became obvious that BP (1985) cross-section data have deficits for use in advanced space-borne ozone measurements. At the same time, it was recognized that the origin of systematic differences in ground-based measurements of ozone required further investigation, in particular whether the BP (1985) cross-section data might contribute to these differences. In ACSO, different sets of laboratory ozone absorption cross-section data (including their dependence on temperature) of the group of Reims (France) (Brion et al., 1993, 1998, 1992, 1995, abbreviated as BDM, 1995) and those of Serdyuchenko et al. (2014), and Gorshelev et al. (2014), (abbreviated as SER, 2014) were examined for use in atmospheric ozone measurements in the Huggins band. In conclusion, ACSO recommends:(a) The spectroscopic data of BP (1985) should no longer be used for retrieval of atmospheric ozone measurements.(b) For retrieval of ground-based instruments of total ozone and ozone profile measurements by the Umkehr method performed by Brewer and Dobson instruments data of SER (2014) are recommended to be used. When SER (2014) is used, the difference between total ozone measurements of Brewer and Dobson instruments are very small and the difference between Dobson measurements at AD and CD wavelength pairs are diminished.(c) For ground-based Light Detection and Ranging (LIDAR) measurements the use of BDM (1995) or SER (2014) is recommended.(d) For satellite retrieval the presently widely used data of BDM (1995) should be used because SER (2014) seems less suitable for retrievals that use wavelengths close to 300 nm due to a deficiency in the signal-to-noise ratio in the SER (2014) dataset.The work of ACSO also showed: The need to continue laboratory cross-section measurements of ozone of highest quality. The importance of careful characterization of the uncertainties of the laboratory measurements. The need to extend the scope of such studies to other wavelength ranges (particularly to cover not only the Huggins band but also the comparison with the mid-infrared region). The need for regular cooperation of experts in spectral laboratory measurements and specialists in atmospheric (ozone) measurements.

Orphal, Johannes↗

Recovery: Fast and Slow—Vegetation Response During the 2012–2016 California Drought

The 2012–2016 California Drought severely impacted natural vegetation across a wide range of environmental gradient. Although several studies have reported an increase in plant water stress and mortality, the spatiotemporal variations of ecosystem productivity responses and the associated environmental and biological drivers remain unclear. Here, using Enhanced Vegetation Index from the Moderate resolution imaging spectrometer, we found that 45% of the natural ecosystems showed an abrupt change (breakpoint [BP]) in productivity during 2012–2016. There were three major contrasting temporal patterns of productivity responses: (i) a steady increase under higher temperature followed by a decline due to accumulated moisture depletion (high elevation forest) or temperature decrease (high elevation nonforest), (ii) gradual decline during the drought followed by a rapid recovery within 1 year after drought stress was partially relieved, and (iii) both a gradual decline and an abrupt decline. The magnitude of abrupt changes was negatively correlated (r = −0.80, p < 0.001) with initial gradual changes. Overall, changes during BP offset, on average, 57% of the preceding gradual responses. The spatial variability in ecosystem response patterns is driven by both environmental and biological factors. Particularly, for forests, positive BP was driven by increasing rainfall and decreasing temperature, while negative BP was mainly driven by the precipitation anomaly. By 2019, 33% of the natural vegetation have recovered to the level of EVI in 2010. Ecosystem responses to multiyear droughts can influence ecosystem dynamics in a complex pattern. Multiple ecohydrological factors should be considered to understand and predict the long-term drought impacts on ecosystems.

Xi Yang↗

Initial Validation of a Gas-Granular Flow Solver Using a Subscale, Reduced Pressure Plume Surface Interaction Ground Test

With NASA’s goal to land the next humans on the lunar surface in the next few years, it is vitally important to have a better understanding of the plume surface interaction (PSI) between the landing vehicles and the lunar regolith. The Fluid Dynamics Branch at NASA/MSFC has previously used the gas-granular flow solver Loci/GGFS to qualitatively predict crater formation due to PSI effects in a lunar (near vacuum) ambient environment. In this paper, validation of Loci/GGFS crater width and depth predictions in ambient near-lunar conditions are provided using experimental data collected at MSFC during the Physics-Focused Ground Test 1 (PFGT-1) campaign in 2022. To observe sensitivity to soil models, simulations were conducted with both monodisperse glass bead (MGB) and BP-1 lunar regolith simulant soil models in Loci/GGFS. Crater depth and width comparisons are made with PFGT-1 Run 56, which used BP-1 soil. The Loci/GGFS BP-1 soil model performed slightly better with a mean predicted crater depth within 10% of the experiment. Both soil models predicted crater width within 10%. Cratering occurred more quickly with the MGB soil model. Mesh and spatial order sensitivity are also examined for the BP-1 soil model.

Validation↗

On the evolution of the lunar orbit

It is generally accepted that the earth-moon separation is at present increasing due to tidal dissipation. Values for the corresponding lunar deceleration and the related slowing of the earth's rotation are obtained from astronomical observations and studies of ancient eclipses. Extrapolation of these values leads to a close approach of the earth and moon 1-3 b.y. BP. Periodicities in the Precambrian stromatolites may yield the number of solar days in a lunar month prior to 500 m.y. BP. These data combined with dynamic constraints on the number of solar days in a lunar month indicate a close approach of the earth and moon at 2.85 plus or minus 0.25 b.y. BP. Mare volcanism on the moon and high-temperature Archean volcanism on the earth prior to this date may be due to tidal heating. Strong tidal heating during a close approach could have contributed to the formation of the first living organisms.

Turcotte, D. L.↗

Holocene submergence of southern Long Island, New York

Samples of basal peat from south-central Long Island have been dated by the radiocarbon method, and a local curve of submergence has been obtained for the past 8,000 yr. The submergence curve indicates that from 7,000 and 3,000 yr BP the Long Island coast was being submerged at a rate of about 25 cm per 100 yr, a rate which slowed markedly to about 10 cm per 100 yr during the past 3,000 yr. Sea-level data for before 7,000 yr show considerable scatter. Samples from the fringing and mid-bay marsh areas of the Great South Bay taken at depths of -1.1 and -0.3 m MSL gave radiocarbon ages of about 1,015 and 300 yr, respectively. The inception of these marshes is estimated to have occurred at about 2,000 yr BP. It seems likely that the change from open lagoon to saltmarsh was initiated in the Great South Bay by a decrease in the rate of submergence at about 3,000 yr BP, which allowed sedimentation to build the floor of the lagoon to a level suitable for colonization by marsh grasses.

Rampino, M. R.↗

Inversion of surface parameters using fast learning neural networks

A neural network approach to the inversion of surface scattering parameters is presented. Simulated data sets based on a surface scattering model are used so that the data may be viewed as taken from a completely known randomly rough surface. The fast learning (FL) neural network and a multilayer perceptron (MLP) trained with backpropagation learning (BP network) are tested on the simulated backscattering data. The RMS error of training the FL network is found to be less than one half the error of the BP network while requiring one to two orders of magnitude less CPU time. When applied to inversion of parameters from a statistically rough surface, the FL method is successful at recovering the surface permittivity, the surface correlation length, and the RMS surface height in less time and with less error than the BP network. Further applications of the FL neural network to the inversion of parameters from backscatter measurements of an inhomogeneous layer above a half space are shown.

Dawson, M. S.↗

Sea ice classification using fast learning neural networks

A first learning neural network approach to the classification of sea ice is presented. The fast learning (FL) neural network and a multilayer perceptron (MLP) trained with backpropagation learning (BP network) were tested on simulated data sets based on the known dominant scattering characteristics of the target class. Four classes were used in the data simulation: open water, thick lossy saline ice, thin saline ice, and multiyear ice. The BP network was unable to consistently converge to less than 25 percent error while the FL method yielded an average error of approximately 1 percent on the first iteration of training. The fast learning method presented can significantly reduce the CPU time necessary to train a neural network as well as consistently yield higher classification accuracy than BP networks.

Dawson, M. S.↗