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At least 55 records · Page 3

Visual suppression of the vestibulo-ocular reflex during space flight

Visual suppression of the vestibulo-ocular reflex was studied in 16 subjects on 4 Space Shuttle missions. Eye movements were recorded by electro-oculography while subjects fixated a head mounted target during active sinusoidal head oscillation at 0.3 Hz. Adequacy of suppression was evaluated by the number of nystagmus beats, the mean amplitude of each beat, and the cumulative amplitude of nystagmus during two head oscillation cycles. Vestibulo-ocular reflex suppression was unaffected by space flight. Subjects with space motion sickness during flight had significantly more nystagmus beats than unaffected individuals. These susceptible subjects also tended to have more nystagmus beats before flight.

Uri, John J.↗

The pituitary - Aging and spaceflown rats

Decrements in growth hormone (GH) release we observed in two spaceflight experiments and four tail-suspended rat studies mimic age-associated changes in the mammalian pituitary GH system seen by Meites and others. The spaceflight data suggest that formation of high molecular weight bioactive disulfide-linked aggregates of the 20 and 22K monomeric GH forms may be reduced in microgravity, thereby, reducing target tissue activity. Correlative studies to confirm spaceflight as a model for pituitary GH system aging should include: (1) investigation of mechanisms of intracellular hormone packaging, (2) consequences to biological activity of the hormone molecule, and (3) study of intracellular microtubule dynamics.

Hymer, W. C.↗

Synchronized Flashing Lights For Approach And Docking

Proposed optoelectronic system for guiding vehicle in approaching and docking with another vehicle includes active optical targets (flashing lights) on approached vehicle synchronized with sensor and image-processing circuitry on approaching vehicle. Conceived for use in automated approach and docking of two spacecraft. Also applicable on Earth to manually controlled and automated approach and docking of land vehicles, aircraft, boats, and submersible vehicles, using GPS or terrestrial broadcast time signals for synchronization. Principal advantage: optical power reduced, with consequent enhancement of safety.

Book, Michael L.↗

Availability of the B beta(15-21) epitope on cross-linked human fibrin and its plasmic degradation products

The binding of radiolabeled monoclonal antifibrin antibody 59D8 (specific for fibrin but not fibrinogen) to a series of degraded fibrin clots showed that the availability of the B beta(15-21) epitope (against which 59D8 had been raised) was inversely proportional to the extent of clot lysis. Examination of digest supernatants revealed that the B beta(15-21) epitope was released from clots as a high molecular weight degradation product in the presence of calcium ions but that the generation of low molecular weight peptides occurred in the absence of calcium ions. To address the question of epitope accessibility, we compared levels of fibrin clot binding among four radioactively labeled antibodies: antifibrin monoclonal antibody 59D8, two antifibrinogen monoclonal antibodies that cross-reacted with fibrin, and an affinity-purified polyclonal antifibrinogen antibody. We expected that the antifibrinogen antibodies would show enhanced binding to clots in comparison with the antifibrin antibody. However, the epitope accessibility experiments showed that all four antibody preparations bound fibrin clots at comparable levels. Taken together, these studies demonstrated that one fibrin-specific epitope, B beta(15-21), remains available on clots as they undergo degradation by plasmin and, importantly, that the epitope is not solubilized at a rate faster than the rate at which the clot is itself solubilized. The availability of the B beta(15-21) epitope during the course of plasminolysis assures the potential utility of antifibrin antibodies such as 59D8 for detecting thrombi and targeting plasminogen activators.

NASA Discipline Regulatory Physiology↗

Earth's Earliest Ecosystems in the C: The Use of Microbial Mats to Demonstrate General Principles of Scientific Inquiry and Microbial Ecology

Microbial mats are living examples of the most ancient biological communities on Earth. As Earth's earliest ecosystems, they are centrally important to understanding the history of life on our planet and are useful models for the search for life elsewhere. As relatively compact (but complete) ecosystems, microbial mats are also extremely useful for educational activities. Mats may be used to demonstrate a wide variety of concepts in general and microbial ecology, including the biogeochemical cycling of elements, photosynthesis and respiration, and the origin of the Earth's present oxygen containing atmosphere. Microbial mats can be found in a number of common environments accessible to teachers, and laboratory microbial mats can be constructed using materials purchased from biological supply houses. With funding from NASA's Exobiology program, we have developed curriculum and web-based activities centered on the use of microbial mats as tools for demonstrating general principles in ecology, and the scientific process. Our web site (http://microbes.arc.nasa.gov) includes reference materials, lesson plans, and a "Web Lab", featuring living mats maintained in a mini-aquarium. The site also provides information as to how research on microbial mats supports NASA's goals, and various NASA missions. A photo gallery contains images of mats, microscopic views of the organisms that form them, and our own research activities. An animated educational video on the web site uses computer graphic and video microscopy to take students on a journey into a microbial mat. These activities are targeted to a middle school audience and are aligned with the National Science Standards.

Bebout, Brad M.↗

First Results from EUNIS-06

The Extreme Ultraviolet Normal-Incidence Spectrograph (EUNIS) sounding rocket experiment successfully completed its first flight on 12 April 2006 from White Sands Missile Range, obtaining 145 science images in each of two wavelength channels. EUNIS is designed to investigate the energetics of the solar corona and hotter transition region through high-resolution imaging spectroscopy with a rapid (2-3 second) cadence. The two independent optical systems of EUNIS simultaneously record spectra over two passbands (170-205 Angstroms and 300-370 Angstroms), each spatially resolved along slit lengths of about 660 arcsec. The longwave channel includes He II 304 Angstroms and strong lines from Fe XI-XVI. The shortwave passband has a sequence of very strong Fe IX-XIII lines. Together, the EUNIS telescopes furnish a wide range of temperature and density diagnostics and enables underflight calibration of instrumental passbands on the SOHO, TRACE, Solar-B and STEREO missions. We present an overview of the science images from the first flight with emphasis on transient phenomena. The target was active region NOAA 10871 and adjacent quiet areas. Spectra were recorded with exposure times as short as 0.1 s, demonstrating that EUNIS is the most sensitive solar EUV spectrograph in operation, with over 100 times the throughput of its predecessor, the Solar Extreme ultraviolet Research Telescope and Spectrograph (SERTS). EUNIS is supported by the NASA Heliophysics Division's Solar & Heliospheric Physics Supporting Research and Technology and Low Cost Access to Space Program.

Rabin, Douglas M.↗

Multi-Source Autonomous Response for Targeting and Monitoring of Volcanic Activity

The study of volcanoes is important for both purely scientific and human survival reasons. From a scientific standpoint, volcanic gas and ash emissions contribute significantly to the terrestrial atmosphere. Ash depositions and lava flows can also greatly affect local environments. From a human survival standpoint, many people live within the reach of active volcanoes, and therefore can be endangered by both atmospheric (ash, debris) toxicity and lava flow. There are many potential information sources that can be used to determine how to best monitor volcanic activity worldwide. These are of varying temporal frequency, spatial regard, method of access, and reliability. The problem is how to incorporate all of these inputs in a general framework to assign/task/reconfigure assets to monitor events in a timely fashion. In situ sensing can provide a valuable range of complementary information such as seismographic, discharge, acoustic, and other data. However, many volcanoes are not instrumented with in situ sensors, and those that have sensor networks are restricted to a relatively small numbers of point sensors. Consequently, ideal volcanic study synergistically combines space and in situ measurements. This work demonstrates an effort to integrate spaceborne sensing from MODIS (Terra and Aqua), ALI (EO-1), Worldview-2, and in situ sensing in an automated scheme to improve global volcano monitoring. Specifically, it is a "sensor web" concept in which a number of volcano monitoring systems are linked together to monitor volcanic activity more accurately, and this activity measurement automatically tasks space assets to acquire further satellite imagery of ongoing volcanic activity. A general framework was developed for evidence combination that accounts for multiple information sources in a scientist-directed fashion to weigh inputs and allocate observations based on the confidence of an events occurrence, rarity of the event at that location, and other scientists' inputs. The software framework uses multiple source languages and is a general framework for combining inputs and incrementally submitting observation requests/reconfigurations, accounting for prior requests. The autonomous aspect of operations is unique, especially in the context of the wide range of inputs that includes manually inputted electronic reports (such as the Air Force Weather Advisories), automated satellite-based detection methods (such as MODVOLC and GOESVOLC), and in situ sensor networks.

Davies, Ashley G.↗

The Hopper: A Wearable Robotic Device Testbed for Micro-Gravity Bone-Loading Proof-of-Concept

Wearable robotic systems are showing increased potential for addressing crew countermeasures needs. Wearable robots offer a compactness, programmability, and eccentric loading capability not present in more conventional exercise equipment. Correspondingly, advancements in the man to machine interface has progressed, allowing for higher loads to be applied directly to the person in new and novel ways. Recently, the X1 exoskeleton, a lower extremity wearable robot originally designed for mobility assistance and rehabilitation, underwent human subject testing to assess its potential as a knee dynamometer. This was of interest to NASA physiologists because currently strength is not assessed in flight due to hardware limitations, and thus there is a poor understanding of the time course of in-flight changes to muscle strength. The study concluded that the X1 compared well with the Biodex, the "gold standard" in terrestrial dynamometry, with coefficients of variation less than 6.0%. In a following study, the X1 powered ankle was evaluated for its efficacy in exercising calf muscles. Current on-orbit countermeasures equipment does not adequately protect the calf from atrophy. The results of this study were also positive (targeted muscle activity demonstrated via comparing pre- and post-exercise magnetic resonance imaging T2 measurements), again showing the efficacy of wearable robotic devices for addressing the countermeasure needs of our astronauts. Based on these successes and lessons learned, the Grasshopper was co-developed between IHMC (Florida Institute for Human and Machine Cognition) and NASA. The Grasshopper, or the Hopper for short, is a wearable robotic device designed to address muscle and bone density loss for astronauts spending extended periods of time in micro-gravity. The Grasshopper connects to the user's torso like a hiking backpack, over the shoulders and around the waist. At the feet are footplates that strap to the user. There are two actuators, one at each "knee" joint, which are capable of high fidelity torque control. Because the Hopper uses motors instead of gravity to create the load on the user, the device is suited for use on space missions. Exercise in zero-gravity conditions is critical to maintain muscle strength and bone mass. In operation, the actuators try to fold up, or collapse, the device, putting a compressive load between the user's feet and torso. This force is similar to carrying a heavy backpack. The user then bends and extends his or her knees, replicating a weightlifting squat exercise. The applied load is precisely controlled by a computer, and can be programmed to simulate gravitation loads or any desired load prescription, such as free-weight squat exercise. It is even possible to perform eccentric exercises, or negatives, without the need for a spotter. Because the hip joints, as well as the spine and long leg bones, are in the applied load path, there is the potential to stimulate bone growth, countering the typical bone loss when astronauts return from extended duration space travel.

Beck, C. E.↗

Guidance and Control Techniques for Titan Aerogravity Assist for Enceladus Observation

Enceladus is a prime scientific target due active geological features and evidence that liquid water might be present on this Saturnian moon. The surface of Enceladus is covered in active fissures known as “Tiger Stripes”. The plumes coming from these rifts were sampled by the Cassini mission, which detected carbon, hydrogen, oxygen, and nitrogen – all key signatures where life could exit. In order to access this moon, mission design using traditional fully-propulsive orbit insertion maneuvers is fuel expensive and time consuming, leading a prior study to rule traditional chemical and solar electric propulsion based missions infeasible. One alternative that enables this mission is Titan aerogravity assist, which is an aeroassist maneuver that combines atmospheric flight and gravity assist of Titan to allow a spacecraft to enter a planetary capture orbit about Saturn. This poster will look at the guidance and control techniques that are required to allow aerogravity assist to take place within Titan’s atmosphere.

Soumyo Dutta↗

Development of a calibration workstation for SIR-C

The paper describes the status of a comprehensive synthetic aperture radar (SAR) software package developed specifically to aid the calibration of a multifrequency and multipolarization radar like the Shuttle Imaging Radar, SIR-C. The primary data types processed are engineering telemetry, point targets (corner reflectors and polarimetric active radar calibrators), distributed targets (clutter), tone-generators, and ground receivers. The parameters derived to aid the calibration of the production processor include measurements of relative radiometric channel to channel imbalance, absolute calibration constants, antenna pattern corrections, noise levels, polarimetric cross-talk, and geometric offsets. The software reports standard performance measurements such as resolution, peak and integrated sidelobes, radiometric response accuracy, and geometrical distortions such as scale errors, position bias and standard deviation, and image skew. Furthermore, the inclusion of software to allow updates of the shuttle-state vector (position and velocity) is considered.

Madsen, S. N.↗

Expression and affinity purification of recombinant proteins from plants

With recent advances in plant biotechnology, transgenic plants have been targeted as an inexpensive means for the mass production of proteins for biopharmaceutical and industrial uses. However, the current plant purification techniques lack a generally applicable, economic, large-scale strategy. In this study, we demonstrate the purification of a model protein, beta-glucuronidase (GUS), by employing the protein calmodulin (CaM) as an affinity tag. In the proposed system, CaM is fused to GUS. In the presence of calcium, the calmodulin fusion protein binds specifically to a phenothiazine-modified surface of an affinity column. When calcium is removed with a complexing agent, e.g., EDTA, calmodulin undergoes a conformational change allowing the dissociation of the calmodulin-phenothiazine complex and, therefore, permitting the elution of the GUS-CaM fusion protein. The advantages of this approach are the fast, efficient, and economical isolation of the target protein under mild elution conditions, thus preserving the activity of the target protein. Two types of transformation methods were used in this study, namely, the Agrobacterium-mediated system and the viral-vector-mediated transformation system. Copyright 2002 Elsevier Science (USA).

Non-NASA Center↗

Extreme Deep Space Communications

Recent work in deep space telecommunication systems has been performed in support of NASA's Mission to the Solar System planning activity. The results show that high bandwidth communications (higher thatn 1 Mbps) are feasible with communication infrastructure investments at targets of high exploration activity. These targets include Mars, Jupiter, and Neptune. Infrastructure improvements must also be made at Earth.

space telecommunications Mission to the Solar Syst↗

TRAC based sensing for autonomous rendezvous

The Targeting Reflective Alignment Concept (TRAC) sensor is to be used in an effort to support an Autonomous Rendezvous and Docking (AR&D) flight experiment. The TRAC sensor uses a fixed-focus, fixed-iris CCD camera and a target that is a combination of active and passive components. The system experiment is anticipated to fly in 1994 using two Commercial Experiment Transporters (COMET's). The requirements for the sensor are: bearing error less than or equal to 0.075 deg; bearing error rate less than 0.3 deg/sec; attitude error less than 0.5 deg.; and attitude rate error less than 2.0 deg/sec. The range requirement depends on the range and the range rate of the vehicle. The active component of the target is several 'kilo-bright' LED's that can emit 2500 millicandela with 40 milliwatts of input power. Flashing the lights in a known pattern eliminates background illumination. The system should be able to rendezvous from 300 meters all the way to capture. A question that arose during the presentation: What is the life time of the LED's and their sensitivity to radiation? The LED's should be manufactured to Military Specifications, coated with silicon dioxide, and all other space qualified precautions should be taken. The LED's will not be on all the time so they should easily last the two-year mission.

Everett, Louis J.↗

The development and validation of command schedules for SeaWiFS

An automated method for developing and assessing spacecraft and instrument command schedules is presented for the Sea-viewing Wide Field-of-view Sensor (SeaWiFS) project. SeaWiFS is to be carried on the polar-orbiting SeaStar satellite in 1995. The primary goal of the SeaWiFS mission is to provide global ocean chlorophyll concentrations every four days by employing onboard recorders and a twice-a-day data downlink schedule. Global Area Coverage (GAC) data with about 4.5 km resolution will be used to produce the global coverage. Higher resolution (1.1 km resolution) Local Area Coverage (LAC) data will also be recorded to calibrate the sensor. In addition, LAC will be continuously transmitted from the satellite and received by High Resolution Picture Transmission (HRPT) stations. The methods used to generate commands for SeaWiFS employ numerous hierarchical checks as a means of maximizing coverage of the Earth's surface and fulfilling the LAC data requirements. The software code is modularized and written in Fortran with constructs to mirror the pre-defined mission rules. The overall method is specifically developed for low orbit Earth-observing satellites with finite onboard recording capabilities and regularly scheduled data downlinks. Two software packages using the Interactive Data Language (IDL) for graphically displaying and verifying the resultant command decisions are presented. Displays can be generated which show portions of the Earth viewed by the sensor and spacecraft sub-orbital locations during onboard calibration activities. An IDL-based interactive method of selecting and testing LAC targets and calibration activities for command generation is also discussed.

Woodward, Robert H.↗

Proximity operations considerations affecting spacecraft design

Proximity operations can be defined as the maneuvering of two or more spacecraft within 1 nautical mile range, with relative velocity less than 10 feet per second. The passive vehicle is nontranslating and should provide for maintenance of the desired approach attitude. It must accommodate the active (translating) vehicle induced structural loads and performance characteristics (mating hardware tolerances), and support sensor compatibility (transponder, visual targets, etc.). The active vehicle must provide adequate sensor systems (relative state information, field-of-view, redundancy), flight control hardware (thruster sizing, minimal cross-coupling, performance margins, redundancy) and software (reconfigurable, attitude/rate modes, translation and rotation fine control authority) characteristic, and adequate non-propulsive consumables such as power. Operational concerns must be considered. These include the following: (1) the desired approach trajectory and relative orientation; (2) the active vehicle thruster plume effects (forces, torques, contamination) on the passive vehicle; and (3) procedures for contingencies such as loss of communications, sensor or propulsion failures, and target vehicle loss of control.

Staas, Steven K.↗

Guidance, Navigation, and Control for Laser-Based Non-Cooperative Target Detumbling

Orbital debris is a well known and growing problem presenting risks to the future of spaceflight. Many pieces of debris are defunct satellites or rocket upper stages. These large objects could generate an exponentially growing cloud of debris should they be involved in a collision, i.e., Kessler Syndrome. Future orbital debris remediation efforts often propose perturbing the orbit of these large targets to mitigate risk. These plans often involve non-cooperative rendezvous. In the absence of active control, the targets are expected to be tumbling which makes rendezvous a dangerous prospect. In this work we describe a guidance, navigation, and control system for a contactless non-cooperative target detumbling methodology which will significantly increase the safety of non-cooperative rendezvous. An onboard laser generates thrust on the target by ablating its surface material. This thrust is used to control target attitude. Our guidance, navigation, and control system is able to estimate relative pose, target the laser, and maintain formation flight during the detumbling process. The system is validated with Monte Carlo simulation in a variety of scenarios.

Guidance↗

Nitric oxide inhibits calpain-mediated proteolysis of talin in skeletal muscle cells

We tested the hypothesis that nitric oxide can inhibit cytoskeletal breakdown in skeletal muscle cells by inhibiting calpain cleavage of talin. The nitric oxide donor sodium nitroprusside prevented many of the effects of calcium ionophore on C(2)C(12) muscle cells, including preventing talin proteolysis and release into the cytosol and reducing loss of vinculin, cell detachment, and loss of cellular protein. These results indicate that nitric oxide inhibition of calpain protected the cells from ionophore-induced proteolysis. Calpain inhibitor I and a cell-permeable calpastatin peptide also protected the cells from proteolysis, confirming that ionophore-induced proteolysis was primarily calpain mediated. The activity of m-calpain in a casein zymogram was inhibited by sodium nitroprusside, and this inhibition was reversed by dithiothreitol. Previous incubation with the active site-targeted calpain inhibitor I prevented most of the sodium nitroprusside-induced inhibition of m-calpain activity. These data suggest that nitric oxide inhibited m-calpain activity via S-nitrosylation of the active site cysteine. The results of this study indicate that nitric oxide produced endogenously by skeletal muscle and other cell types has the potential to inhibit m-calpain activity and cytoskeletal proteolysis.

NASA Discipline Musculoskeletal↗

Global Positioning System Synchronized Active Light Autonomous Docking System

A Global Positioning System Synchronized Active Light Autonomous Docking System (GPSSALADS) for automatically docking a chase vehicle with a target vehicle comprises at least one active light emitting target which is operatively attached to the target vehicle. The target includes a three-dimensional array of concomitantly flashing lights which flash at a controlled common frequency. The GPSSALADS further comprises a visual tracking sensor operatively attached to the chase vehicle for detecting and tracking the target vehicle. Its performance is synchronized with the flash frequency of the lights by a synchronization means which is comprised of first and second internal clocks operatively connected to the active light target and visual tracking sensor, respectively, for providing timing control signals thereto, respectively. The synchronization means further includes first and second Global Positioning System receivers operatively connected to the first and second internal clocks, respectively, for repeatedly providing simultaneous synchronization pulses to the internal clocks, respectively. In addition, the GPSSALADS includes a docking process controller means which is operatively attached to the chase vehicle and is responsive to the visual tracking sensor for producing commands for the guidance and propulsion system of the chase vehicle.

Howard, Richard↗