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At least 55 records · Page 3

Structure and sequence evolution in the pennycress ( Thlaspi arvense ) pangenome

Eukaryotic genomes harbor many forms of variation, including nucleotide diversity and structural polymorphisms, which experience natural selection and contribute to genome evolution and biodiversity. Harnessing this variation for agriculture hinges on our ability to detect, quantify, catalog, and deploy genetic diversity. Here, we explore seven complete genomes of the emerging biofuel crop pennycress ( Thlaspi arvense ) drawn from across the species' current genetic diversity to catalog variation in genome structure and content. Across this new pangenome resource, we find contrasting evolutionary modes in different genomic zones. Gene-poor, repeat-rich pericentromeric regions experience frequent rearrangements, including repeated centromere repositioning. By contrast, conserved gene-dense chromosome arms maintain large-scale synteny across accessions even in fast-evolving NOD-like receptor immune genes, where microsynteny breaks down across species, but gene cluster positioning macrosynteny is maintained. Our findings highlight that multiple elements of the genome experience dynamic evolution that conserves functional content on the chromosome scale but allows repositioning and presence–absence variation on a local scale. This diversity is invisible to classical reference-based strategies and highlights the strength and utility of pangenomic resources. These results provide a valuable case study of rapid genomic structural evolution within a species and powerful resources for crop development in an emerging biofuel crop.

Thlaspi arvense↗

Structural Insight into a Sixteen Cyanine Dye Construct via Exciton–Exciton Annihilation

Molecular (dye) aggregates play a prominent role in light harvesting and are of interest in quantum information science; however, there are limited reports hat programmably assemble many (>4) dye aggregates featuring strong coupling and exciton delocalization. Using oligonucleotides with four Cy5s covalently linked in series along the phosphate backbone, we bring four, eight, and sixteen Cy5s in close proximity by assembling four-armed junctions. We elucidate their structure via gel electrophoresis and steady-state and transient optical spectroscopy. We find that Cy5 has a strong propensity to form tetramer clusters, where the exciton is delocalized over all four Cy5s, that the exciton is not delocalized beyond tetramer clusters in the eight and sixteen Cy5 constructs, and that the sixteen Cy5 construct may consist of pairs of tetramer clusters that are isolated from one another. Many-dye aggregates such as these may serve useful as antennae for their intense light absorption and spatially directed energy transfer.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Dependence of Cumulus Anvil Radiative Properties on Environmental Conditions in the Tropical West Pacific

Areally extensive, optically thick anvil clouds associated with mesoscale convective clusters dominate the shortwave cloud forcing in the tropics and provide longwave forcing comparable to that of thin cirrus. Changes in the cover and optical thickness of tropical anvils as climate warms can regulate the sign of cloud feedback. As a prelude to the study of MMCR data from the ARM TWP sites, we analyze ISCCP-derived radiative characteristics of anvils observed in the tropical west Pacific during the TOGA-COARE IOP. Anvils with radius greater than 100 km were identified and tracked from inception to decay using the Machado-Rossow algorithm. Corresponding environmental conditions just prior to the start of the convectove event were diagnosed using the Lin-Johnson objective analysis product. Small clusters (100-200 km radius) are observed to have a broad range of optical thicknesses (10-50), while intermediate optical thickness clusters are observed to range in size from 100 km to almost 1000 km. Large-size clusters appear to be favored by strong pre-storm large scale upward motion throughout the troposphere, moist low-to-midlevel relative humidities, environments with slightly higher CAPE than those for smaller clusters, and strong front-to-rear flow. Optically thick anvils are favored in situations of strong low-level moisture convergence and strong upper-level shear.

Ye, B.↗

The X-ray properties of normal galaxies

The results of an Einstein Observatory study of 51 nearby normal galaxies, including the Large and Small Magellanic Clouds and M31, are reported. The X-ray luminosity of normal galaxies is about 0.0002 of the optical luminosity and shows no strong correlation with morphological type. Approximately 30 new supernova remnants were recognized in the Magellanic Clouds. Over 90 sources were detected in M31, of which at least 20 are identified with globular clusters. The number of luminous sources detected in the nearest galaxies per unit mass are similar to the number found in the Galaxy. Individual X-ray sources in the arms of nearby spirals can be very luminous; seven with luminosities in excess of 10 to the 39th ergs/s have been found. The nuclei of some, but not all, normal galaxies are luminous X-ray sources. Possible reasons for this emission are considered.

Long, K. S.↗

CO observations around galactic longitude l=45 deg

An area of about 1.5 deg x 2.0 deg, centered on l=45.5 deg and b=0.0 deg, was mapped in the (C-12)O line at intervals of one beamwidth. A total of 22 individual CO cloud complexes was identified; the brightest of these is associated with a massive H I cloud and the H II region complex G45.5+0.1. This object most likely represents a small OB star cluster in its early stages of development. All other H II regions in the area mapped are likewise associated with CO maxima. The survey results indicate a structure of the Sagittarius arm generally in agreement with that derived in earlier studies; they also indicate that the Galaxy as a whole contains of the order of 3500 molecular clouds larger than 10 pc in diameter.

Israel, F. P.↗

High-resolution IRCCD images of the Galactic center at 2.2 and 3.5 microns

An IRCCD camera was used to obtain high-resolution images at 2.2 and 3.5 microns of the Galactic center at subarcsecond resolution. These images show that the compact nonthermal radio source Sgr A-asterisk is not coincident with any part of IRS 16; Sgr A-asterisk is found to be 1.1 + or - 0.3 arcsec west of IRS 16C. At 3.5 microns, IRS 16 is a complex object. IRS 16SW is resolved into two sources, and IRS 16C may be double or extended. From the colors and sizes of the various components, it is difficult to explain these objects as stellar clusters; the required stellar density would be too high. Finally, there is evidence that there is a blue and a red source, separated by about 1 arcsec, for each of the northern arm sources, IRS 1W, IRS 5, and IRS 10.

Tollestrup, Eric V.↗

Metagenomic compendium of 189,680 DNA viruses from the human gut microbiome

Bacteriophages have important roles in the ecology of the human gut microbiome but are under-represented in reference databases. To address this problem, we assembled the Metagenomic Gut Virus catalogue that comprises 189,680 viral genomes from 11,810 publicly available human stool metagenomes. Over 75% of genomes represent double-stranded DNA phages that infect members of the Bacteroidia and Clostridia classes. Based on sequence clustering we identified 54,118 candidate viral species, 92% of which were not found in existing databases. The Metagenomic Gut Virus catalogue improves detection of viruses in stool metagenomes and accounts for nearly 40% of CRISPR spacers found in human gut Bacteria and Archaea. We also produced a catalogue of 459,375 viral protein clusters to explore the functional potential of the gut virome. This revealed tens of thousands of diversity-generating retroelements, which use error-prone reverse transcription to mutate target genes and may be involved in the molecular arms race between phages and their bacterial hosts.

59 BASIC BIOLOGICAL SCIENCES↗

Plan Position Indicator Hydrometeor Field Statistics (PPIHYD) Evaluation Data Product Version 1.0

The PPIHYD evaluation data product provides distinct hydrometeor field statistics calculated from U.S. Department of Energy Atmospheric Radiation Measurement (ARM) user facility scanning radar plan position indicator (PPI) scans. These statistics include the equivalent reflectivity factor and Doppler spectral width percentiles, min/max values, and first four moments (mean, standard deviation, skewness, and kurtosis) of distinct hydrometeor features (clustered hydrometeor fields). Statistics also include morphological properties, water content and precipitation rate parameterization-based estimates, and thermodynamic properties interpolated using the Interpolated Sonde value-added product (INTERPSONDE VAP). The data set is organized in tabular form and is accompanied by mask arrays with corresponding indices. This straightforward file structure simplifies scanning radar data processing and renders this data set useful for process understanding and model evaluation studies. This report describes the data set and its processing algorithm and provides some examples.

54 ENVIRONMENTAL SCIENCES↗

Frequency-Selectable Laser Source (FLS) Calibrator for CMB Bandpass Characterization

One of the biggest challenges for Cosmic Microwave Background (CMB) experiments comes from our detector bandpass calibration. Uncertainties in bandpass can severely limit our measurements by limiting foreground removal and spectral fitting, which is particularly important for high-$\ell$ observations like cluster science using the Sunyaev-Zeldovich (SZ) effect. Currently, CMB experiments typically use a Fourier Transform Spectrometer (FTS) to measure the detector bandpasses. However, the resolution of the FTS is dependent on the length of the interferometer arms, leading to a need for increasingly large FTS instruments as CMB experiments require tighter constraints on detector bandpasses. Additionally, systematic effects like shifts in bandpass shape from uneven illumination from the FTS further limit the calibration uncertainties. As a complement to the FTS, we have developed a Frequency-selectable Laser Source (FLS) calibrator, which uses a laser with adjustable frequency housed in a calibrator that allows for varying degrees of laser power attenuation. We present several tests used to characterize the first prototype design of the FLS calibrator, as well as the improvements to the calibrator design currently underway.

Saunders, Lauren [Fermilab]↗

Hubble Space Telescope Imaging of the Circumnuclear Environments of the CfA Seyfert Galaxies: Nuclear Spirals and Fueling

We present archival Hubble Space Telescope (HST) images of the nuclear regions of 43 of the 46 Seyfert galaxies found in the volume limited,spectroscopically complete CfA Redshift Survey sample. Using an improved method of image contrast enhancement, we created detailed high-quality " structure maps " that allow us to study the distributions of dust, star clusters, and emission-line gas in the circumnuclear regions (100-1000 pc scales) and in the associated host galaxy. Essentially all of these Seyfert galaxies have circumnuclear dust structures with morphologies ranging from grand-design two-armed spirals to chaotic dusty disks. In most Seyfert galaxies there is a clear physical connection between the nuclear dust spirals on hundreds of parsec scales and large-scale bars and spiral arms in the host galaxies proper. These connections are particularly striking in the interacting and barred galaxies. Such structures are predicted by numerical simulations of gas flows in barred and interacting galaxies and may be related to the fueling of active galactic nuclei by matter inflow from the host galaxy disks. We see no significant differences in the circumnuclear dust morphologies of Seyfert 1s and 2s, and very few Seyfert 2 nuclei are obscured by large-scale dust structures in the host galaxies. If Sevfert 2s are obscured Sevfert Is, then the obscuration must occur on smaller scales than those probed by HST.

Pogge, Richard W.↗

Detailed X-ray observations of M83

X-ray observation of M83 with the Einstein Observatory indicates an extent and shape for this emission that are comparable to those in the blue band, although the inner 2-arcmin region shows a relative X-ray excess that may be accounted for by a clustering of bulge-type X-ray sources in the inner region of the plane of the galaxy. A lack of correlation in high resolution X-ray observations is noted between X-ray emission and the spiral arm pattern, suggesting that most of the X-ray emission from the plane of M83 is due to unresolved sources, belonging to the 'smooth' disk population, whose ages are in the 100 million-1 billion year range. Attention is given to six bright sources in the plane and nuclear region of M83, whose high X-ray luminosities suggest that they are close accreting binaries.

Trinchieri, G.↗

Automated characterization of spatial and dynamical heterogeneity in supercooled liquids via implementation of machine learning

Abstract A computational approach by an implementation of the principle component analysis (PCA) with K -means and Gaussian mixture (GM) clustering methods from machine learning algorithms to identify structural and dynamical heterogeneities of supercooled liquids is developed. In this method, a collection of the average weighted coordination numbers ( W C N s ‾ ) of particles calculated from particles’ positions are used as an order parameter to build a low-dimensional representation of feature (structural) space for K -means clustering to sort the particles in the system into few meso-states using PCA. Nano-domains or aggregated clusters are also formed in configurational (real) space from a direct mapping using associated meso-states’ particle identities with some misclassified interfacial particles. These classification uncertainties can be improved by a co-learning strategy which utilizes the probabilistic GM clustering and the information transfer between the structural space and configurational space iteratively until convergence. A final classification of meso-states in structural space and domains in configurational space are stable over long times and measured to have dynamical heterogeneities. Armed with such a classification protocol, various studies over the thermodynamic and dynamical properties of these domains indicate that the observed heterogeneity is the result of liquid–liquid phase separation after quenching to a supercooled state.

75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND↗

Far-ultraviolet Variable Sources in M31

The Ultraviolet Imaging Telescope (UVIT) on board the AstroSat observatory has imaged the Andromeda galaxy (M31) from 2017 to 2019 in the far- and near-UV (FUV and NUV) with the high spatial resolution of ≃1″. The survey covered the large sky area of M31 with a set of observations (Fields), each 28′ in diameter. Field 1 was observed in two epochs with the F148W filter, separated by ≃1133 days (≃3.10 yr). The 6.4 kpc diameter Field 1 (at the distance of M31) includes a substantial part of the inner spiral arms of the galaxy. We identify UVIT sources in both epochs of Field 1 and obtain catalogs of sources that are variable in FUV at >3σ and >5σ confidence level. The fraction of FUV-variable sources is higher for brighter sources, and the fraction is higher in the two main spiral arms compared to other areas. This is evidence that a significant fraction of the FUV variables are associated with hot young stars. Source counterparts are found for 42 of the 86 >5σ FUV variables using existing catalogs. The counterparts include 10 star clusters, 6 H II regions, 5 regular or semiregular variables, 6 other variables, and 6 nova or nova candidates. The UVIT FUV–NUV and FUV–FUV color–magnitude diagrams confirm the association of most of the FUV variables with hot young stars. A catalog of UVIT photometry for the variable sources is presented.

47 OTHER INSTRUMENTATION↗

How Well Does the DOE Global Storm Resolving Model Simulate Clouds and Precipitation Over the Amazon?

This study assesses a 40-day 3.25-km global simulation of the Simple Cloud-Resolving E3SM Model (SCREAMv0) using high-resolution ground-based observations from the Atmospheric Radiation Measurement (ARM) Green Ocean Amazon (GoAmazon) field campaign. SCREAMv0 reasonably captures the diurnal timing of boundary layer clouds yet underestimates the boundary layer cloud fraction and mid-level congestus. SCREAMv0 well replicates the precipitation diurnal cycle, however it exhibits biases in the precipitation cluster size distribution compared to scanning radar observations. Specifically, SCREAMv0 overproduces clusters smaller than 128 km, and does not form enough large clusters. Such biases suggest an inhibition of convective upscale growth, preventing isolated deep convective clusters from evolving into larger mesoscale systems. This model bias is partially attributed to the misrepresentation of land-atmosphere coupling. This study highlights the potential use of high-resolution ground-based observations to diagnose convective processes in global storm resolving model simulations, identify key model deficiencies, and guide future process-oriented model sensitivity tests and detailed analyses.

54 ENVIRONMENTAL SCIENCES↗

Experiments to validate Thermodynamics and Transport models of Strongly Coupled Dusty Plasma Matter (Final Technical Report for DE-SC0023416)

The goal of this two-year grant is to provide access to the PI to dusty plasma experimental facilities at the DOE-funded Collaborative Research Facility Magnetized Plasma Research Laboratory, Auburn University to become a user of that facility, to obtain experimental data to support another ongoing grant DE-SC0021146 (an Early Career Award to the PI that is focused on modeling of dusty plasma thermodynamics and transport processes), to generate experimental data for funding proposals, and to provide exposure to University of Memphis students to advanced experimental techniques. The following technical accomplishments were made: 1. Development of a novel Bidirectional Electrode Control Arms Assembly (BECAA) for producing perfect 2D grain layers for complex plasma experimentation. BECAA uses movable electrode arms to tilt or move the electrode in a RF discharge from outside the chamber, allowing for the manipulation of grain clouds without needing to change the plasma parameters or gas pressure. This work addresses a longstanding gap in the literature for a method to produce clusters of selectable number of grains and that are perfectly two dimensional as opposed to being only quasi-2D. 2. Experimental investigation of the structural properties of finite-N clusters with N=2 to 50. Individual particle behavior in clusters could vary from grain to grain and this study measured systematically produced clusters for two different grain sizes. Analysis (funded by another grant DE-SC0021146) is currently underway to quantify the differences between grains that are found on the surface vs. the interior of clusters, the shell structure, and the decay of correlations in position, velocity, and kinetic energy. 3. An experimental method to measure the structural entropy of clusters was developed by observing the self-induced structural transitions between various possible arrangements. In a series of heating and cooling cycles, the number of times each possible arrangement was attained was experimentally observed and used to compute the probability of existence of that arrangement, and subsequently the configurational entropy of the cluster. Analysis (funded by another grant DE-SC0021146) is currently underway to produce the entropy of clusters as a function of the number of grains and use the same to compute thermodynamic state variables for 2D complex plasma/grain clusters. 4. A preliminary experimental study of multibody collisions between N grains (N=2 – 10) was conducted. The clusters were produced using the BECAA technique and velocities were imparted to the grains using manipulation laser pulses. Analysis (funded by another grant DE-SC0021146) is currently underway to develop a theoretical framework to describe multibody collisions analogous to classical two-body interactions.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

Long- and short-term temporal variability in cloud condensation nuclei spectra over a wide supersaturation range in the Southern Great Plains site

Abstract. When aerosol particles seed the formation of liquid water droplets in the atmosphere, they are called cloud condensation nuclei (CCN). Different aerosols will act as CCN under different degrees of water supersaturation (relative humidity above 100 %), depending on their size and composition. In this work, we build and analyze a best-estimate CCN spectrum product, tabulated at ∼ 45 min resolution, generated using high quality data from seven independent instruments at the U.S. Department of Energy Atmospheric Radiation Measurement (ARM) Southern Great Plains site. The data product spans a large supersaturation range, from 0.0001 % to ∼ 30 %, and time period of 5 years, from 2009–2013, and is available on the ARM data archive. We leverage this added statistical power to examine relationships that are unclear in smaller datasets. Our analysis is performed in three main areas. First, probability distributions of many aerosol and CCN metrics are found to exhibit skewed log-normal distribution shapes. Second, clustering analyses of CCN spectra reveal that the primary drivers of CCN differences are aerosol number size distributions, rather than hygroscopicity or composition, especially at supersaturations above 0.2 %, while also allowing for a simplified understanding of seasonal and diurnal variations in CCN behavior. The predictive ability of using limited hygroscopicity data with accurate number size distributions to estimate CCN spectra is investigated, and the uncertainties of this approach are estimated. Third, the dynamics of CCN spectral clusters and concentrations are examined with cross-correlation and autocorrelation analyses. We find that CCN concentrations change rapidly on the timescale of 1–3 h, with some conservation beyond that which is greatest for the lower supersaturation region of the spectrum.

54 ENVIRONMENTAL SCIENCES↗

Characterization of novel recombinant mycobacteriophages derived from homologous recombination between two temperate phages

Comparative analyses of mycobacteriophage genomes reveals extensive genetic diversity in genome organization and gene content, contributing to widespread mosaicism. We previously reported that the prophage of mycobacteriophage Butters (cluster N) provides defense against infection by Island3 (subcluster I1). To explore the anti-Island3 defense mechanism, we attempted to isolate Island3 defense escape mutants on a Butters lysogen, but only uncovered phages with recombinant genomes comprised of regions of Butters and Island3 arranged from left arm to right arm as Butters-Island3-Butters (BIBs). Recombination occurs within two distinct homologous regions that encompass lysin A, lysin B, and holin genes in one segment, and RecE and RecT genes in the other. Structural genes of mosaic BIB genomes are contributed by Butters while the immunity cassette is derived from Island3. Consequently, BIBs are morphologically identical to Butters (as shown by transmission electron microscopy) but are homoimmune with Island3. Recombinant phages overcome antiphage defense and silencing of the lytic cycle. We leverage this observation to propose a stratagem to generate novel phages for potential therapeutic use.

59 BASIC BIOLOGICAL SCIENCES↗

Wrist joint assembly

A wrist joint assembly is provided for use with a mechanical manipulator arm for finely positioning an end-effector carried by the wrist joint on the terminal end of the manipulator arm. The wrist joint assembly is pivotable about a first axis to produce a yaw motion, a second axis is to produce a pitch motion, and a third axis to produce a roll motion. The wrist joint assembly includes a disk segment affixed to the terminal end of the manipulator arm and a first housing member, a second housing member, and a third housing member. The third housing member and the mechanical end-effector are moved in the yaw, pitch, and roll motion. Drive means are provided for rotating each of the housings about their respective axis which includes a cluster of miniature motors having spur gears carried on the output drive shaft which mesh with a center drive gear affixed on the housing to be rotated.

Kersten, L.↗