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SPRUCE Bud Cold Hardiness of Trees and Shrubs in Experimental Plots, Marcell Experimental Forest, Minnesota, 2021-2025

This dataset contains bud cold hardiness measurements from the Spruce and Peatland Responses Under Changing Environments (SPRUCE) experimental site (Hanson et al, 2017) within the Marcell Experimental Forest in northern Minnesota, USA. Cold hardiness was quantified as the temperature at which low temperature exotherms (LTEs) were identified (i.e., the temperature at which supercooled water within a bud freezes) measured by differential thermal analysis (DTA). Buds were sampled at semi-regular intervals from September 2021 through May 2025 (2021-09-25 to 2025-05-21) across four seasons (2021-2022 through 2024-2025) for four co-occurring boreal peatland tree and shrub species: Picea mariana (PIMA), Larix laricina (LALA), Rhododendron groenlandicum (RHGR), and Chamaedaphne calyculata (CHCA). Samples were collected across the experiment's warming gradient (ambient to +9 degrees Celsius (C)) and pooled by species within each enclosure. These data were used to assess the species- and season-specific effects of experimental warming on cold hardiness and cold damage risk in boreal peatland vegetation (Campos-Arguedas et al, accepted). This dataset contains one data file in comma-separate values (*.csv) format. Additional metadata are provided: a data dictionary and a file-level metadata file in comma-separate values (*.csv) format and a user guide in PDF (*.pdf) format.

Chamaedaphne calyculata↗

Electricity Baseline 2021 Background Data and Log File

The ElectricityLCI v2 Python package (https://github.com/USEPA/ElectricityLCI/tree/v2.0) was used to generate the 2021 electricity baseline: a regionalized life cycle inventory model of U.S. electricity generation, consumption, and distribution using standardized facility and generation data. ElectricityLCI implements a local data store for downloading and accessing public data on an individual's computer. The data store follows the folder definition provided by USEPA's esupy Python package (https://github.com/USEPA/esupy), which utilizes the appdirs Python dependency (https://pypi.org/project/appdirs/). An overview of the ElectricityLCI data stores may be found on the README (https://github.com/USEPA/ElectricityLCI/blob/v2.0/README.md#data-store). This submission includes the background data used to generate the 2021 electricity baseline inventory. Each zip archive stores the source files as found in their data stores. Sub-folders in each of the data stores are archived separately. For example, stewi.zip contains the JSON files, while stewi.facility.zip is the 'facility' sub-folder of stewi data store that stores the parquet files. To reproduce the data store, extract each zip file and drag-and-drop sub-folders in to their appropriate root folders to recreate the data stores, then copy the root folders to your data store folder (as returned by running the following on the command line: python -c "import appdirs; print(appdirs.user_data_dir())"). The main five data stores include: 'electricitylci', 'facilitymatcher', 'fedelemflowlist', 'stewi', and 'stewicombo'. The log file generated by the 2021 model run is also included, which contains the statements at the DEBUG level and above.

Electricity; LCA; LCI; Life Cycle; data inventory↗

2021 Consequence Management Hotline Drill After Action Report

In March 2021, a functional area drill was held at the Remote Sensing Laboratory–Nellis that focused on using CBRNResponder and the Digital Field Monitoring (DFM) tablets for sample hotline operations and the new paper Sample Control Forms (SCFs) for sample collection. Participants included staff trained and billeted as sample control specialists and Consequence Management Response Team (CMRT) field monitoring personnel. Teams were able to successfully gather and transfer samples to the sample control hotline staff through the manual process, though there were several noted areas for improvement. In July and October 2021, two additional functional area drills were held at Sandia National Laboratories that focused on field sample collection and custody transfer at the sample control hotline for the Consequence Management (CM) Radiological Assistance Program (RAP) program. The overarching goal of the drills was to evaluate the current CM process for sample collection, sample drop off, and sample control using the CBRNResponder mobile and web-based applications. The July 2021 drill had an additional focus to have a subset of samples analyzed by the local analytical laboratory, Radiation Protection Sample Diagnostics (RPSD) laboratory, to evaluate the Laboratory Access portal on CBRNResponder. All three drills were able to accomplish their objectives however, there were several issues noted (Observations: 25 Urgent, 29 Important, and 22 Improvement Opportunities). The observations were prioritized according to their impact on the mission as well as categorized to align with the programmatic functional area required to address the issue. This report provides additional detail on each observation for skillset/program leads and software developers to consider for future improvement or mandatory efforts.

96 KNOWLEDGE MANAGEMENT AND PRESERVATION↗

Idaho National Laboratory CY 2021 National Emission Standards for Hazardous Air Pollutants Analysis, Methodology and Results for Radionuclides

This report details calculations of potential dose at public receptor locations surrounding the Idaho National Laboratory (INL) Site boundary, and INL in-town facilities, from radionuclides reported to be in use and potentially emitted from INL facilities during calendar year (CY) 2021. All calculations were performed in accordance with the requirements in Code of Federal Regulations (CFR), Title 40, “Protection of the Environment,” Part 61, “National Emission Standards for Hazardous Air Pollutants (NESHAPs),” Subpart H, “National Emission Standards for Emissions of Radionuclides Other than Radon from Department of Energy Facilities” (40 CFR 61, Subpart H). Modeling methodology, model input parameters, and contribution to dose by facility, source, and radionuclide at the maximally exposed individual (MEI) location are also discussed. The information in this report supports the “National Emission Standards for Hazardous Air Pollutants - Calendar Year 2021 INL Report for Radionuclides” (INL 2022). In CY 2021, the estimated annual potential dose at the INL Site MEI location was 6.67E-02 mrem/yr, up slightly from the previous year, but far less than the regulatory standard of 10 mrem/yr (CFR 40 Part 61, Subpart H). Approximately 97% of the total dose to the INL Site MEI originated from MFC sources. Emissions from INL in-town facilities resulted in an estimated annual potential dose of 6.21E-03 mrem/yr to the MEI, down 40% from the CY 2020 estimated dose. Year-to-year variations in estimated annual dose can be attributed to adjustments in laboratory operations, changes to facility infrastructure, and variation in meteorological conditions.

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗

Financial Analysis of Experimental Releases Conducted at Glen Canyon Dam during Water Year 2021

This report was prepared by Argonne National Laboratory (Argonne) in support of a financial analysis of two Glen Canyon Dam (GCD) flow experiments that were conducted in Water Year 2021. The first experiment, called “spring disturbance flow”, was conducted from March 15 to 26, 2021. This disturbance flow experiment is designed to simulate a spring-timed runoff event so scientists can study its effects on the Colorado River ecosystem. The second experiment was a reduced and steady discharge from May 29 to June 4, 2021. This reduced steady flow experiment was conducted for the United States Geological Survey (USGS) to collect high-resolution aerial imagery over Grand Canyon National Park. This analysis was funded by the Colorado River Storage Project (CRSP) Office of the U.S. Department of Energy’s Western Area Power Administration (WAPA). CRSP markets electricity produced by hydroelectric facilities collectively known as the Salt Lake City Area Integrated Projects including dams equipped for power generation on the Colorado, Green, Gunnison, and Rio Grande Rivers and on Plateau Creek in the states of Arizona, Colorado, New Mexico, Utah, and Wyoming. Staff members in Argonne’s Energy Systems Division prepared this technical memorandum with assistance from WAPA’s CRSP and Energy Marketing and Management Offices (EMMO).

58 GEOSCIENCES↗

Composite Analysis for Low Level Waste Disposal in the Central Plateau of the Hanford Site (Annual Status Report, FY 2021)

In accordance with DOE M 435.1-1 requirements and as implemented by DOE/RL-2000-29, the U.S. Department of Energy (DOE), Richland Operations Office has prepared this annual summary for fiscal year (FY) 2021. Originally reported in PNNL-11800 and PNNL-11800, Addendum 1 (hereinafter collectively referred to as the Hanford Site Composite Analysis), the Hanford Site Composite Analysis was approved through issuance of a 2002 memorandum. As required by DOE/RL-2000-29, an annual evaluation of new information and data developed by a number of onsite programs was completed. The reporting period for this annual evaluation is FY 2021 (October 1, 2020 through September 30, 2021).

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

US Department of Energy, Office of Science High Performance Computing Facility Operational Assessment 2021: Oak Ridge Leadership Computing Facility

Oak Ridge National Laboratory’s (ORNL’s) Leadership Computing Facility (OLCF) continues to surpass its operational target goals of supporting users; delivering fast, reliable computational ecosystems; creating innovative solutions for high-performance computing (HPC) needs; contributing to the community to build the next generation HPC workforce, and managing risks, safety, and security associated with operating some of the most powerful computers in the world. The results can be seen in the cutting-edge science conducted by users and the praise from the research community. Calendar year (CY) 2021 saw continued excellence in research supported by the OLCF’s leadership-class computing resources, including Summit (the nation’s most powerful supercomputer), the global scratch file system Alpine, the Scalable Protected Infrastructure (SPI), the Exploratory Visualization Environment for Research in Science and Technology (EVEREST), and the archival mass-storage resource High-Performance Storage System (HPSS). While maintaining access and exceptional user support for Summit, the OLCF continued to make progress on the installation and deployment of Frontier, which will be the nation’s first exascale system when it comes online at the start of CY 2023. Users have already begun running and optimizing scientific codes on Crusher, the OLCF test and development system equipped with Frontier’s architecture. Throughout the year, the OLCF maintained a strong culture of operational excellence, including risk management, workplace safety, and cybersecurity. The OLCF’s rigorous risk management strategy anticipated and mitigated risks, and at this time there are no high-priority operational risks. Similarly, ORNL and the OLCF were committed to operating under the US Department of Energy’s (DOE’s) safety regulations that ensure a safe workplace. Technical staff tracked and monitored existing threats and vulnerabilities within the OLCF while continually developing tools and practices to enhance operations without increasing the facility’s risk. CY 2021 was filled with outstanding results and accomplishments, including a very high rating from users on overall satisfaction for the eighth consecutive year; a tremendous number of node hours delivered to 1,671 researchers on Summit; and the successful delivery of the allocation split of roughly 60%, 20%, and 20% of core-hours offered for the Innovative and Novel Computational Impact on Theory and Experiment (INCITE), Advanced Scientific Computing Research Leadership Computing Challenge (ALCC), and Director’s Discretionary (DD) programs, respectively (Section 2). COVID-19 research remained a focus in 2021, and the ALCC and DD programs allocated over 1 million Summit hours to the COVID-19 High Performance Computing Consortium. These accomplishments, coupled with the high utilization rates (i.e., overall and capability usage), represent the fulfillment of the promise of leadership class machines: efficient facilitation of leadership-class computational applications.

97 MATHEMATICS AND COMPUTING↗

Performance Assessment for the Environmental Restoration Disposal Facility (Annual Status Report FY 2021)

DOE O 435.1 and DOE M 435.1-1 require that a determination of continued adequacy of the performance assessment (PA) (CP-60089), composite analysis (CA), and disposal authorization statement (DAS) be made on an annual basis, and that the determination must consider the results of data collection and analysis from research, field studies, and monitoring as well as the need to update any Radioactive Waste Management Basis (RWMB) documents. Beginning in 1996, the Environmental Disposal Facility (ERDF) started accepting low-level radioactive, hazardous, and mixed wastes that were generated during cleanup activities at the Hanford Site. ERDF is composed of a series of cells or disposal areas and can accommodate future design expansions as needed. Currently, there are eight cells and two supercells in ERDF. Each supercell is the equivalent of two cells. During this reporting period (fiscal year 2021, extending from October 1, 2020, through September 30, 2021), approximately 9.14E+04 metric tons (1.01E+05 U.S. tons) of waste was disposed at ERDF. From ERDF inception through September 30, 2021, approximately 16.9 million metric tons (18.9 U.S. tons) of waste has been disposed of at ERDF, which equates to consumption of approximately 88.7% of the currently constructed disposal volume. According to the design of ERDF, the facility has the ability to be expanded as needed. As a condition of the DAS, disposal operation within ERDF must be in accordance with the waste acceptance criteria (ERDF-00011) that provide specific radionuclide disposal limits, waste form restrictions, and descriptions of acceptable waste packages in compliance with the requirements of DOE M 435-1.1. The ERDF waste acceptance criteria stipulate that waste destined for disposal at ERDF be controlled based on source, physical form, and contaminant concentration and activity levels. There have been no changes to the physical configuration of ERDF or to the waste forms (source, physical form, etc.). No new unreviewed disposal question screenings or evaluations have been generated in this reporting period. Therefore, there are no noted impacts to the PA, CA, DAS, or RWMB resulting from the evaluations and screenings. Sum-of-fractions analysis shows that the disposed inventory meets both the concentration and inventory threshold requirements. A sum-of-fractions value is computed for ERDF sensitive radionuclides contributing to the all pathways and the air pathway inventory limits. Computed values were 7.63E-03 and 1.49E-03, respectively. The disposed waste inventory remained well under the PA imposed limits, as shown in Table 4 and Table 5 in the main text of this report. Required monitoring was satisfactorily completed during the fiscal year reporting period. Compliance with performance objectives were met as each of the reported values were well below the established limit. Overall, there are no substantive changes to primary PA assumptions or changes to the PA analysis conclusion; therefore, compliance with DOE O 435.1 and the DAS is maintained.

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

PNNL Richland Campus Radionuclide Air Emissions Report for Calendar Year 2021

This report documents radionuclide air emissions that result in the 2021 highest effective dose equivalent (EDE) to an offsite member of the public, referred to as the maximally exposed individual (MEI). The report has been prepared in compliance with the Code of Federal Regulations, Title 40, Protection of the Environment, Part 61, National Emission Standards for Hazardous Air Pollutants, Subpart H, “National Emission Standards for Emissions of Radionuclides Other than Radon from Department of Energy Facilities” and Washington Administrative Code Chapter 246-247, “Radiation Protection–Air Emissions.” The dose to the PNNL Richland Campus MEI from routine major and minor point source emissions in 2021 from PNNL Richland Campus sources is 1.7E-5 mrem (1.7E-7 mSv) EDE. The dose from all fugitive sources is 1.2E-6 mrem (1.2E-8 mSv) EDE. The dose from radon emissions is 2.1E-9 mrem (2.1E-11 mSv) EDE. No nonroutine emissions occurred in 2021. The total radiological dose to the MEI from all PNNL Richland Campus radionuclide emissions, including fugitive emissions and radon, is 1.8E-5 mrem (1.8E-7 mSv) EDE, or more than 100,000 times less than the federal and state standard of 10 mrem/yr, with which the PNNL Richland Campus is in compliance.

40 CFR 61 Subpart H↗

Idaho National Laboratory’s FY 2021 Greenhouse Gas Report

A greenhouse gas (GHG) inventory is a systematic approach to account for the production and release of certain gases generated by an institution from various emission sources. The gases of interest are those that climate science has identified as related to anthropogenic global climate change. This document presents an inventory of GHGs generated during Fiscal Year (FY) 2021 by Idaho National Laboratory (INL)—a Department of Energy (DOE) sponsored entity located in southeastern Idaho. In recent years, concern has grown about the environmental impact of GHGs. This, together with a desire to decrease harmful environmental impacts, would be enough to encourage the calculation of an inventory of the total GHGs generated at INL. Additionally, INL has a desire to see how its emissions compare with similar institutions, including other DOE national laboratories. Executive Order 13834 requires that federal agencies and institutions track and report GHG emissions where required. INL’s GHG inventory was calculated according to methodologies identified in federal GHG guidance documents using operational control boundaries. It measures emissions generated in three scopes: (1) INL emissions produced directly by stationary or mobile combustion and by fugitive emissions, (2) the share of emissions generated by entities from which INL purchased electrical power, and (3) indirect or shared emissions generated by outsourced activities that benefit INL (occurring outside INL’s organizational boundaries but are a consequence of INL’s activities). This inventory found that INL generated 81,185.05 metric tons (MT) of CO 2 equivalent (CO 2 e) emissions during FY 2021. The following conclusions were made from looking at the results of the individual contributors to INL’s FY 2021 GHG inventory: Electricity (including the associated transmission and distribution losses) is the largest contributor to INL’s GHG inventory, with over 50% of the CO 2 e emissions; Other sources with high emissions were mobile combustion (fleet fuels), employee commuting, stationary combustion (facility fuels), and waste disposal (fugitive emissions from the onsite landfill); Sources with low emissions were waste disposal (contracted disposal), fugitive emissions from refrigerants, wastewater treatment (onsite and contracted), and business ground travel (in personal and rental vehicles). This report details the methods behind quantifying INL’s GHG inventory and discusses lessons learned on better practices by which information important to tracking GHGs can be tracked and recorded. It is important to note that because this report differentiates between those portions of INL that are managed and operated by Battelle Energy Alliance, LLC (BEA) and those managed by other contractors, it includes only INL’s activities overseen by BEA. It is assumed that other contractors will provide similar reporting for those activities they manage, where appropriate.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

Analysis Program 2021 Annual Progress Report

During fiscal year 2021 (FY 2021), the U.S. Department of Energy Vehicle Technologies Office (VTO) funded analysis projects supportive of VTO’s goals to pursue early-stage research in vehicle and mobility system technologies to reduce petroleum dependence, increase energy reliability and security, improve transportation affordability, and promote economic growth. VTO analysis projects result in a foundation of data, analytical models, and applied analyses that provide insights into critical transportation energy problems and assist in research investment prioritization and portfolio planning. This document presents a brief overview of VTO analysis efforts and progress for projects funded in FY 2021. Each of the progress reports includes project objectives, approach, and highlights of the technical results that were accomplished during the fiscal year (FY).

33 ADVANCED PROPULSION SYSTEMS↗

Batteries (2021 Annual Progress Report)

This document summarizes the progress of VTO battery R&D projects supported during the fiscal year 2021 (FY 2021). In FY 2021, the DOE VTO battery R&D funding was approximately $\$$115 million. Its R&D focus was on the development of high-energy batteries for EVs as well as very high-power devices for hybrid vehicles. The electrochemical energy storage roadmap (which can be found at the EERE Roadmap web page2) describes ongoing and planned efforts to develop electrochemical storage technologies for EVs. To advance battery technology, which can in turn improve market penetration of PEVs, the program investigates various battery chemistries to overcome specific technical barriers, e.g., battery cost, performance, life (both the calendar life and the cycle life), its tolerance to abusive conditions, and its recyclability/sustainability. VTO R&D has had considerable success, lowering the cost of EV battery packs to $\$$185/kWh in 2019 (representing more than 80% reduction since 2008) yet even further cost reduction is necessary for EVs to achieve head-to-head cost competitiveness with ICEs (without Federal subsidies). In addition, today’s batteries also need improvements in such areas as their ability to accept charging at a high rate, referred to as extreme fast charging (XFC) (15 minute charge) – to provide a “refueling” convenience similar to ICEs, and the ability to operate adequately at low temperatures. Research into “next-gen lithium-ion” batteries which would provide such functionalities is one of the R&D focus areas. VTO is funding research on both “next gen” chemistries (which employ an alloy anode and/or a high voltage cathode) and beyond lithium-ion (BLI) chemistries (which can, for example, employ a lithium metal anode).

25 ENERGY STORAGE↗

Electrification 2021 Annual Progress Report

During fiscal year 2021 (FY 2021), the U.S. Department of Energy (DOE) Vehicle Technologies Office (VTO) funded early-stage research, development, demonstration, & deployment (RDD&D) projects that address Batteries and Electrification of the U.S. transportation sector. The VTO Electrification Sub-Program is composed of Electric Drive Technologies, and Grid Integration activities. The Electric Drive Technologies group conducts R&D projects that advance electric motors and power electronics technologies. The Grid and Charging Infrastructure group conducts R&D projects that advance grid modernization and electric vehicle charging technologies. This document presents a brief overview of the Electrification Sub-Program and progress reports for its R&D projects. Each of the progress reports provide a project overview and highlights of the technical results that were accomplished in fiscal year (FY) 2021.

33 ADVANCED PROPULSION SYSTEMS↗

Hanford Site Revegetation Monitoring Report for Fiscal Year 2021

This report describes the monitoring of areas revegetated by the River Corridor Closure Contractor (RCCC) and CH2M Hill Plateau Remediation Company (CHPRC) that were transitioned to Mission Support Alliance (MSA) in 2017. These sites, along with sites revegetated by MSA between 2017 and 2020, were transitioned to Hanford Mission Integration Solutions (HMIS) in 2021. Site monitoring is a continuance of efforts performed by the RCCC from fiscal year (FY) 2007 through FY 2020. This report contains data collected in 2021 documenting the recovery of revegetation areas associated with the Comprehensive Environmental Response, Compensation, and Liability Act of 1980 cleanup of National Priorities List waste sites and restoration of lands disturbed by ongoing Site mission activities at the Hanford Site in Richland, Washington. It contains vegetation monitoring data for 52 sites selected to be representative sites for areas planted between the years of FY 2007 and FY 2021.

54 ENVIRONMENTAL SCIENCES↗

Feedstock-Conversion Interface Consortium Annual Review of Research – FY 2021

Led by the U.S. Department of Energy’s Bioenergy Technologies Office, the Feedstock-Conversion Interface Consortium (FCIC) is a multi-lab collaboration that develops first-principles-based knowledge and tools to understand, quantify, and mitigate the effects of feedstock and process variability across the bioenergy value chain, from the field and forest through downstream conversion. The FCIC recently published its Annual Review of Research Fiscal Year (FY) 2021 report, which presents an overview of the research accomplishments of FCIC members in FY 2021, covering both the low- and high-temperature conversion pathways and all three process areas: Feedstock harvest and storage Preprocessing Conversion. The key research achievements of each task are presented, along with planned FY 2022 work that will build on the FY 2021 accomplishments.

09 BIOMASS FUELS↗

Precursor Analysis Report: Blackmatter Ransomware Attack on New Cooperative 2021

The BlackMatter Ransomware Attack on New Cooperative 2021 Precursor Analysis Report leverages publicly available information about the New Cooperative cyber attack and catalogs anomalous observables for each technique employed in the attack. This analysis is based upon the methodology of the Cybersecurity for the Operational Technology Environment (CyOTE) program. The BlackMatter ransomware was first identified in July 2021 and is reported to have infected more than 50 corporations around the world. , The Iowa-based grain cooperative, New Cooperative, was impacted by the BlackMatter ransomware on or before 18 September 2021. The adversary likely resided on New Cooperative’s networks for 15 days prior to encrypting its network and demanding New Cooperative pay $5.9 million in ransom by 25 September to unlock systems and prevent 1 terabyte (TB) of sensitive data from being publicly released. It is not clear if New Cooperative paid the ransom. The full impact of the ransomware attack is not known; however, according to New Cooperative’s general manager, the attack caused the company’s automated processes to revert back to processes used in the 1970s. , As of 6 October, only 50 percent of New Cooperative’s operations were utilizing automated processes. The company took eight weeks to rebuild the entire network and information technology (IT) systems from the ground up, which puts the date of fully recovery around 13 November. Researchers and analysts identified 20 unique techniques utilized during the attack with a total of 404 observables using MITRE ATT&CK® for Industrial Control Systems. The CyOTE program assesses observables accompanying techniques used prior to the triggering event to identify opportunities to detect malicious activity. If observables accompanying the attack techniques are perceived and investigated prior to the triggering event, earlier comprehension of malicious activity can take place. Seventeen of the identified techniques used during the New Cooperative cyber attack were precursors to the triggering event. Analysis identified 360 observables associated with these precursor techniques, 284 of which were assessed to have an increased likelihood of being perceived in the 15 days preceding the triggering event. The response and comprehension time could have been reduced if the observables had been identified earlier. The information gathered in this report contributes to a library of observables tied to a repository of artifacts, data sources, and technique detection references for practitioners and developers to support the comprehension of indicators of attack. Asset owners and operators can use these products if they experience similar observables or to prepare for comparable scenarios.

45 MILITARY TECHNOLOGY, WEAPONRY, AND NATIONAL DEF↗

SPRUCE Ground Observations of Phenology in Experimental Plots, 2021

This data set consists of one comma separated (*.csv) file containing phenological transition dates, as derived from direct observations of vegetative and reproductive phenology recorded by a human observer, from the SPRUCE experiment during 2021, the sixth full year of whole-ecosystem warming (Hanson et al. 2017). Both spring and autumn phenological events are included. Since April 2016, human observers have been directly tracking the phenology of both woody and herbaceous species on a weekly schedule within the SPRUCE experimental chambers, these data are reported in annual ground observations data sets (see Related Data Sets). The observed date reported here is the first survey date in 2021 on which an event/phenophase was definitively observed. This data set also contains a companion file in HTML (*.html) format containing figures showing the relationship between the day of year and temperature treatment for different phenological phases by species for 2021. User note: Ground observations of phenology from 2016-2020 are available. See the user guide for links to all other ground phenology datasets.

54 ENVIRONMENTAL SCIENCES↗

SPRUCE Sphagnum Growth and Photosynthesis Responses to Shading Treatments, 2021

This dataset reports growth, water content, nitrogen concentration, and photosynthesis of Sphagnum grown under shade cloth of different density in replicated plots adjacent to the Spruce and Peatland Responses Under Changing Environments (SPRUCE) experimental study plots located in the S1-Bog. Data are reported for May to October 2021. Investigations were instigated from questions arising in the SPRUCE experiment where the Sphagnum-shrub-spruce/larch ecosystem is exposed to air and peat warming in combination with elevated atmospheric CO2. SPRUCE is located at the 8.1-ha S1 Bog forest site in northern Minnesota, 40 km north of Grand Rapids, in the USDA Forest Service Marcell Experimental Forest (MEF). Thirty plots (35 × 35 cm) were established in May, 2021, in three blocks on unused large plots on the S-1 bog. Data are reported for growth of Sphagnum angustifolium/fallax and S. divinum growing in plastic columns within the shade plots. Growth is reported as dry mass of new tissue measured when the columns were harvested in October 2021. Additional measurements in the final harvest datasetinclude Sphagnum water content, new stem length, mass per unit length, and nitrogen content. Stem extension was measured periodically during the summer and reported in a separate datasetas stem length. Photosynthesis of Sphagnum angustifolium/fallax samples from hollows under low and high shade was measured in the laboratory. Responses of sphagnum to shading are important for understanding the future of peatland systems under the encroachment of shrubs and other woody plant species. This dataset contains three data files in comma-separated values (.csv) format. Additional metadata are provided: three data dictionaries and a file-level metadata file in comma-separated values (.csv) format and a user guide in PDF (*.pdf) format.

carbon dioxide flux↗