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The NASA Artemis program has brought significant change to the agency’s human exploration strategy over the last several years. The human exploration of Mars remains an ultimate objective of the overall human exploration strategy. However, how the agency intends to execute initial human exploration of Mars has shifted to better align with current policy. These changes find their way into the design of key architecture elements, such as the Mars Ascent Vehicle (MAV). Of the numerous changes in the conceptual human Mars architecture, two in particular have had significant impact on the design of the human MAV: the desire for minimal surface infrastructure for initial human Mars missions, and technology investment timelines in support of initial human missions to Mars. The first leads to a surface mission architecture targeting a 30-day surface duration with two crewmembers. The second drives out in-situ resource utilization (ISRU) from the initial sortie. As a result, the reference MAV design for initial human Mars missions in the Human Exploration and Operations Mission Directorate’s Strategic Analysis Cycle 2021 (SAC21) is based around supporting the return of 2 crew from the surface of Mars, without surface ISRU. This has led to several design changes since 2019, when details of a reference human MAV were last published. The MAV concept for SAC21 consists of a two-stage vehicle capable of supporting two crew for 84 hours. This duration is derived from a baseline ascent trajectory targeting an apoapsis rendezvous with the habitation element in a nominal 5-sol Mars aggregation orbit. With the goal of minimizing technology investments for the initial surface sortie removing ISRU from the trade space, the MAV must now be landed either fully loaded with propellant, or with some portion of the propellant off-loaded. Landing a partially-fueled MAV will require additional surface assets to perform robotic propellant transfer on the surface of Mars from a pre-emplaced propellant depot. Furthermore, cryogenic fluid management and storage for extended duration with no losses represents additional technology investment that would have to be made to support cryogenic-based propellant concepts that have been the baseline in past architectures. As a result, the SAC21 architecture utilizes a storable propellant-based MAV propulsion system to minimize these potential technology investments incurred by cryogenic-based propulsion systems. Recent efforts also focused on evaluating the sensitivity of key design parameters, such as landing site latitude, elevation, and local atmospheric conditions. All play a large role in determining the predicted propellant requirements of the MAV. Understanding their potential impacts is important because of the rippling effects of changes to the MAV design on other architecture elements, such as entry, descent, landing, and transportation systems. Results indicate up to +2% to -4% wet mass variation due to landing site latitude, with higher latitudes greater than 70 degrees North resulting in greater than 6% wet mass increases. The MAV saw reduced impact due to atmospheric design parameters, which only accounting for -0.6% to +0.4% wet mass variation.