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At least 505 records · Page 28
All sourcesNASA NTRSDOE OSTI
NASA NTRS1999

Analysis of MIR Condensate and Potable Water

Approximately fifty percent of the potable water supplied to the Russian cosmonauts, American astronauts, and other occupants of the current Russian Mir Space Station is produced by the direct recycle of water from humidity condensate. The remainder comes from ground supplied potable water that is delivered on a Progress resupply spacecraft, or processed fuel cell water transferred from the Shuttle. Reclamation of water for potable and hygiene purposes is considered essential for extended duration missions in order to avoid massive costs associated with resupplying water from the ground. The Joint U.S/Russian Phase 1 program provided the U.S. the first opportunity to evaluate the performance of water reclamation hardware in microgravity. During the Phase I program, the U.S. collected recycled water, stored water, and humidity condensate samples for chemical and microbial evaluation. This experiment was conducted to determine the potability of the water supplied on Mir, to assess the reliability of the water reclamation and distribution systems, and to aid in developing water quality monitoring standards for International Space Station.

Pierre, L. M.↗
NASA NTRS2003

Life in the Ice

The current Martian surface environment is extremely hostile to any known form of life. The combination of subfreezing temperature, low atmospheric pressure and high ultraviolet flux, combined with desiccated and possibly oxidizing soil, could destroy even the hardiest microorganisms. The Viking biology experiments are generally interpreted to indicate that the surface of Mars is currently devoid of life and organic molecules at the part-per-billion level. Speculation on the possibility of extant or preserved microbial life on Mars thus centers on refuges in some manner protected from the current surface environment, either in space or time. Terrestrial analogs include hydrothermal systems, lakes, caves and subsurface aquifers as well as more clement conditions in the distant past. We are examining the evidence for microbiology in Earth's glaciated polar regions as analogs to the polar caps of Mars. This research concerns the detection of microorganisms or their preserved remains at the surface and within polar glacial ice.

C C Allen↗
NASA NTRS1992

Effects of refrigerating preinoculated Vitek cards on microbial physiology and antibiotic susceptibility

Reference cultures of 16 microorganisms obtained from the American Type Culture Collection and four clinical isolates were used in standardized solutions to inoculate 60 cards for each test strain. A set of three ID and three susceptibility cards was processed in the Vitek AutoMicrobic System (AMS) immediately after inoculation. The remaining cards were refrigerated at 4 C, and sets of six cards were removed and processed periodically for up to 17 days. The preinoculated AMS cards were evaluated for microorganism identification, percent probability of correct identification, length of time required for final result, individual substrate reactions, and antibiotic minimal inhibitory/concentration (MIC) values. Results indicate that 11 of the 20 microbes tested withstood refrigerated storage up to 17 days without detectable changes in delineating characteristics. MIC results appear variable, but certain antibiotics proved to be more stable than others. The results of these exploratory studies will be used to plan a microgravity experiment designed to study the effect of microgravity on microbial physiology and antibiotic sensitivity.

Skweres, Joyce A.↗
NASA NTRSDate not supplied

SPACEFLIGHT-INDUCED CHANGES IN MICROBIAL VIRULENCE AND THE IMPACT TO THE HOST IMMUNE RESPONSE

INTRODUCTION Over the past 50 years, microorganisms have displayed unexpected responses relevant to infectious disease when grown in microgravity and microgravity analogue environments, including changes in final cell concentration, biofilm production, stress resistance, antibiotic sensitivity, gene expression, and virulence. In parallel, astronaut studies have characterized a persistent spaceflight-induced dysregulation of the human immune system; consisting of altered leukocyte distribution, reductions in T and NK cell function, altered cytokine profiles, and reactivation of latent herpesviruses. Further, astronauts have some degree of clinical incidence, primarily infectious disease episodes and atopic dermatitis. The impact of the microgravity environment on host-pathogen interactions and potential for clinical disease remains understudied and poorly characterized. SPECIFIC AIMS In the Host-Microorganism, Microbial Response aspect of this study, the following Specific Aims are being investigated, using the microbial pathogens, Salmonella enterica Enteritidis, Pseudomonas aeruginosa, Burkholderia cepacia, Streptococcus pneumoniae, and enterohemorrhagic Escherichia coli. Aim 1: Characterize the effect of spaceflight analogue culture on microbial pathogenesis-related stress responses and in vitro host-pathogen interactions. Analysis will include microbial stress responses as well as colonization and viability following pathogen challenge of three-dimensional (3-D) tissue co-culture models containing immune cells. We specifically selected obligate and opportunistic pathogens that are medically important and have been or are likely to be found aboard spacecraft. Aim 2: Characterize the effect of spaceflight analogue culture on the virulence potential of pathogenic microorganisms. Changes in virulence will be assessed using a mouse model of infection. SIGNIFICANCE The goal of this study is to use spaceflight analogue conditions to gain insight into the breadth of medically-significant International Space Station microorganisms that have altered virulence and the impact of those changes on the immune response of the host, including astronaut immune cells. This information will provide critical understanding into the impact of microgravity on potential alterations in microbial virulence and associated infectious disease risk to crew health during spaceflight missions.

C. M. Ott↗
NASA NTRS2001

Deep UV Native Fluorescence Imaging of Antarctic Cryptoendolithic Communities

An interdisciplinary team at the Jet Propulsion Laboratory Center for Life Detection has embarked on a project to provide in situ chemical and morphological characterization of Antarctic cryptoendolithic microbial communities. We present here in situ deep ultraviolet (UV) native fluorescence and environmental scanning electron microscopy images transiting 8.5 mm into a sandstone sample from the Antarctic Dry Valleys. The deep ultraviolet imaging system employs 224.3, 248.6, and 325 nm lasers to elicit differential fluorescence and resonance Raman responses from biomolecules and minerals. The 224.3 and 248.6 nm lasers elicit a fluorescence response from the aromatic amino and nucleic acids. Excitation at 325 nm may elicit activity from a variety of biomolecules, but is more likely to elicit mineral fluorescence. The resultant fluorescence images provide in situ chemical and morphological maps of microorganisms and the associated organic matrix. Visible broadband reflectance images provide orientation against the mineral background. Environmental scanning electron micrographs provided detailed morphological information. The technique has made possible the construction of detailed fluorescent maps extending from the surface of an Antarctic sandstone sample to a depth of 8.5 mm. The images detect no evidence of microbial life in the superficial 0.2 mm crustal layer. The black lichen component between 0.3 and 0.5 mm deep absorbs all wavelengths of both laser and broadband illumination. Filamentous deep ultraviolet native fluorescent activity dominates in the white layer between 0.6 mm and 5.0 mm from the surface. These filamentous forms are fungi that continue into the red (iron-rich) region of the sample extending from 5.0 to 8.5 mm. Using differential image subtraction techniques it is possible to identify fungal nuclei. The ultraviolet response is markedly attenuated in this region, apparently from the absorption of ultraviolet light by iron-rich particles coating the filaments. Below 8.5 mm the filamentous morphology of the upper layers gives way to punctate 1-2 micron particles evidencing fluorescent activity following excitation at both deep ultraviolet wavelengths.

Storrie-Lombardi, M. C.↗
NASA NTRS2004

Characterization of microbial and chemical composition of shuttle wet waste with permanent gas and volatile organic compound analyses

Solid-waste treatment in space for Advanced Life Support, ALS, applications requires that the material can be safely processed and stored in a confined environment. Many solid-wastes are not stable because they are wet (40-90% moisture) and contain levels of soluble organic compounds that can contribute to the growth of undesirable microorganisms with concomitant production of noxious odors. In the absence of integrated Advanced Life Support systems on orbit, permanent gas, trace volatile organic and microbiological analyses were performed on crew refuse returned from the volume F "wet" trash of three consecutive Shuttle missions (STS-105, 109, and 110). These analyses were designed to characterize the short-term biological stability of the material and assess potential crew risks resulting from microbial decay processes during storage. Waste samples were collected post-orbiter landing and sorted into packaging material, food waste, toilet waste, and bulk liquid fractions deposited during flight in the volume F container. Aerobic and anaerobic microbial loads were determined in each fraction by cultivation on R2A and by acridine orange direct count (AODC). Dry and ash weights were performed to determine both water and organic content of the materials. Experiments to determine the aerobic and anaerobic biostability of refuse stored for varying periods of time were performed by on-line monitoring of CO2 and laboratory analysis for production of hydrogen sulfide and methane. Volatile organic compounds and permanent gases were analyzed using EPA Method TO15 by USEPA et al. [EPA Method TO15, The Determination of Volatile Organic Compounds (VOCs) in Ambient Air using SUMMA, Passivated Canister Sampling and Gas Chromatographic Analysis,1999] with gas chromatography/mass spectrometry and by gas chromatography with selective detectors. These baseline measures of waste stream content, labile organics, and microbial load in the volume F Shuttle trash provide data for waste subsystem analysis and atmospheric management within the ALS Project. Published by Elsevier Ltd on behalf of COSPAR.

NASA Center KSC↗
NASA NTRS1987

System for Odorless Disposal of Human Waste

Conceptual system provides clean, hygienic storage. Disposal system stores human wastes compactly. Releases no odor or bacteria and requires no dangerous chemicals or unpleasant handling. Stabilizes waste by natural process of biodegradation in which microbial activity eventually ceases and ordors and bacteria reduced to easily contained levels. Simple and reliable and needs little maintenance.

Jennings, Dave↗
NASA NTRS2010

Long Duration Life Test of Propylene Glycol Water Based Thermal Fluid Within Thermal Control Loop

Evaluations of thermal properties and resistance to microbial growth concluded that 50% Propylene Glycol (PG)-based fluid and 50% de-ionized water mixture was desirable for use as a fluid within a vehicle s thermal control loop. However, previous testing with a commercial mixture of PG and water containing phosphate corrosion inhibitors resulted in corrosion of aluminum within the test system and instability of the test fluid. This paper describes a follow-on long duration testing and analysis of 50% Propylene Glycol (PG)-based fluid and 50% de-ionized water mixture with inorganic corrosion inhibitors used in place of phosphates. The test evaluates the long-term fluid stability and resistance to microbial and chemical changes

Le, Hung↗
NASA NTRS2019

The 2008 Mars Phoenix Lander Thermal and Evolved Gas Analyzer (TEGA) Dataset: Placing Easily Interpretable Evolved Gas Data on the Planetary Data System (PDS)

The Phoenix Scout Lander mission investigated the north polar region of Mars in 2008 with the goal to study the history of water, assess the past/present Martian climate, search for organics, and evaluate the potential for past/present microbial habit-ability on Mars. To accomplish this goal, the Phoenix Lander’s Thermal and Evolved-Gas Analyzer (TEGA) instrument assessed the gas composition of the Martian atmosphere and evaluated the mineralogy of the Martian regolith. The TEGA instrument consisted of eight small ovens connected to a 4 channel magnetic sector mass spectrometer. The ovens heated soil samples from ambient to 1000°C where the gases (e.g., H2O, CO2, etc.) evolved from thermal decomposition of mineral phases were analyzed by the mass spectrometer. Minerals thermally decomposed at characteristic temperatures and the evolving gases indicated the presence of perchlorate, carbonate, and hydrated phases in the Phoenix landing site soils.

Garcia, A. H.↗
NASA NTRS2005

Microbial Preservation in Sulfates in the Haughton Impact Structure Suggests Target in Search for Life on Mars

Microbes in Haughton Crater Sulfates: Impact craters are of high interest in planetary exploration because they are viewed as possible sites for evidence of life [1]. Hydrothermal systems in craters are particularly regarded as sites where primitive life could evolve. Evidence from the Miocene Haughton impact structure shows that crater hydrothermal deposits may also be a preferred site for subsequent colonization and hence possible extant life: Hydrothermal sulfates at Haughton are colonized by viable cyanobacteria [2]. The Haughton impact structure, Devon Island, Canadian High Arctic, is a 24 km-diameter crater of mid-Tertiary age. The structure preserves an exceptional record of impact-induced hydrothermal activity, including sulfide, and sulfate mineralization [3]. The target rocks excavated at the site included massive gypsum-bearing carbonate rocks of Ordovician age. Impact-remobilized sulfates occur as metre-scale masses of intergrown crystals of the clear form of gypsum selenite in veins and cavity fillings within the crater s impact melt breccia deposits [4]. The selenite is part of the hydrothermal assemblage as it was precipitated by cooling hot waters that were circulating as a result of the impact. Remobilization of the sulfate continues to the present day, such that it occurs in soil crusts (Fig. 1) including sandy beds with a gypsum cement. The sulfate-cemented beds make an interesting comparison with the sulfate-bearing sandy beds encountered by the Opportunity MER [5]. The selenite crystals are up to 0.3 m in width, of high purity, and transparent. They locally exhibit frayed margins where cleavage surfaces have separated. This exfoliation may be a response to freeze-thaw weathering. The selenite contains traces of rock detritus, newly precipitated gypsum, and microbial colonies. The rock detritus consists of sediment particles which penetrated the opened cleavages by up to 2cm from the crystal margins. Some of the detritus is cemented into place by gypsum, which must have been dissolved and reprecipitated from the host selenite.

Parnell, J.↗
NASA NTRS1996

Comparison of aerobically-treated and untreated crop residue as a source of recycled nutrients in a recirculating hydroponic system

This study compared the growth of potato plants on nutrients recycled from inedible potato biomass. Plants were grown for 105 days in recirculating, thin-film hydroponic systems containing four separate nutrient solution treatments: (1) modified half-strength Hoagland's (control), 2) liquid effluent from a bioreactor containing inedible potato biomass, 3) filtered (0.2 micrometer) effluent, and 4) the water soluble fraction of inedible potato biomass (leachate). Approximately 50% of the total nutrient requirement in treatments 2-4 were provided (recycled) from the potato biomass. Leachate had an inhibitory effect on leaf conductance, photosynthetic rate, and growth (50% reduction in plant height and 60% reduction in tuber yield). Plants grown on bioreactor effluent (filtered or unfiltered) were similar to the control plants. These results indicated that rapidly degraded, water soluble organic material contained in the inedible biomass, i.e., material in leachate, brought about phytotoxicity in the hydroponic culture of potato. Recalcitrant, water soluble organic material accumulated in all nutrient recycling treatments (650% increase after 105 days), but no increase in rhizosphere microbial numbers was observed.

NASA Discipline Life Support Systems↗
NASA NTRS1992

Analysis of debris from Spacelab Space Life Sciences-1

Airborne microbiological and particulate contamination generated aboard Spacelab modules is a potential safety hazard. In order to shed light on the characteristics of these contaminants, microbial and chemical/particulate analyses were performed on debris vacuumed from cabin and avionics air filters in the Space Life Sciences-1 (SLS-1) module of the Space Transportation System 40 (STS-40) mission 1 month after landing. The debris was sorted into categories (e.g., metal, nonmetal, hair/fur, synthetic fibers, food particles, insect fragments, etc.). Elemental analysis of particles was done by energy dispersive analysis of x rays (metals) and Fourier transform infrared spectroscopy (nonmetals). Scanning electron micrographs were done of most particles. Microbiological samples were grown on R2A culture medium and identified. Clothing fibers dominated the debris by volume. Other particles, all attributed to the crew, resulted from abrasions and impacts during missions operations (e.g., paint chips, plastic, electronic scraps and clothing fibers). All bacterial species identified are commonly found in the atmosphere or on the human body. Bacillus sp. was the most frequently seen bacterium. One of the bacterial species, Enterobacter agglomerans, could cause illness in crew members with depressed immune systems.

Caruso, S. V.↗
NASA NTRS2018

EVA Swab Tool to Support Planetary Protection and Astrobiology Evaluations

When we send humans to search for life on other planets, we'll need to know what we brought with us versus what may already be there. To ensure our crewed systems meet planetary protection requirements?and to protect our science from human contamination?we'll need to assess whether microorganisms may be leaking or venting from our spacecraft. Microbial sample collection outside of a pressurized spacecraft is complicated by temperature extremes, low pressures that preclude the use of laboratory standard (wetted) swabs, and operation either in bulky spacesuits or with robotic assistance. A team at the National Aeronautics and Space Administration (NASA) recently developed a swab kit for use in collecting microbial samples from the external surfaces of crewed spacecraft, including spacesuits. The Extravehicular Activity (EVA) Swab Kit consists of a single swab tool handle and an eight-canister sample caddy. The design team minimized development cost by re-purposing a heritage Space Shuttle tile repair handle that was designed to quickly snap into different tool attachments by engaging a mating device in each end effector. This allowed the tool handle to snap onto a fresh swab end effector much like popular shaving razor handles can snap onto a disposable blade cartridge. To disengage the handle from a swab, the user performs two independent functions, which can be done with a single hand. This dual operation mitigates the risk that a swab will be inadvertently released and lost in microgravity. Each swab end effector is fitted with commercially available foam swab tips, vendor-certified to be sterile for Deoxyribonucleic Acid (DNA). A microbial filter installed in the bottom of each sample container allows the container to outgas and re-pressurize without introducing microbial contaminants to internal void spaces. Extensive ground testing, post-test handling, and sample analysis confirmed the design is able to maintain sterile conditions as the canister moves between various pressure environments. To further minimize cost, the design team acquired extensive ground test experience in a relevant flight environment by piggy-backing onto suited crew training runs. These training runs allowed the project to validate tool interfaces with pressurized EVA gloves and collect user feedback on the tool design and function, as well as characterize baseline microbial data for different types of spacesuits. In general, test subjects found the EVA Swab Kit relatively straightforward to operate, but identified a number of design improvements that will be incorporated into the final design. Although originally intended to help characterize human forward contaminants, this tool has other potential applications, such as for collecting and preserving space-exposed materials to support astrobiology experiments.

planetary protection↗
NASA NTRS2017

Impact of Biofilms on the Design and Operation of ISS Life Support Systems

Biofilm growth has been an ongoing issue for US and Russian water systems on the International Space Station, and is a critical issue for exploration missions in which water systems must be designed to accommodate dormant periods of up to one year. On ISS, Russian condensate plumbing has previously clogged with biomass, requiring condensate plumbing to now be regularly replaced. In the US Segment, the release of biofilm from the Water Processor waste tank has clogged a solenoid valve downstream of the tank, resulting in the costly replacement of the inlet separator and process pump. Subsequent management of the biofilm in the waste tank involves restrictions on tank cycles to limit the release of biomass and an additional filter to protect downstream components. Engineering personnel are now evaluating concepts to better manage the biomass, including the use of microbial inhibitors and UV LEDs. Though current ISS operations could likely be sustained for the duration of ISS, a more effective method must be developed for managing the growth and release of biomass in future exploration vehicles. Biofilm management for future missions is complicated by the requirement to accommodate extended periods of dormancy during which time the water system will be stagnant. The current approach under consideration is to flush the waste water with product water to reduce the organic content followed by use of microbial inhibitors or UV. However, other concepts may also be developed based on ongoing research.

Carter, Donald Layne↗
NASA NTRS1986

The Antarctic cold desert and the search for traces of life on Mars

The cryptoendolithic microoganisms that live inside rocks in the frigid Ross Desert of Antarctica can serve as a terrestrial model for what may have happened to life forms on Mars when the planet became dry and cold. Trace fossils of microbial rock colonization exist in Antarctica, and similar structures could have formed on Mars. In some respects, such trace fossils could be an easier target for life-detection systems than fossils of cellular structures.

Friedmann, E. I.↗
NASA NTRS1994

Influence of suspension on the oxidative burst by rat neutrophils

The influence of spaceflight on the oxidative burst of neutrophils is not known. The present study was designed to evaluate the influence of antiorthostatic suspension, a ground-based modeling system designed to simulate certain aspects of weightlessness that occur after spaceflight, on the capacity of rat neutrophils to express the oxidative burst, an important host defense mechanism against microbial pathogens. Rats were suspended in whole body harnesses in the antiorthostatic orientation for a 3- or 7-day period. Control rats were suspended orthostatically or allowed to remain in vivarium cages without the attachment of any suspension materials. After suspension, peripheral blood was harvested and neutrophils were isolated by density gradient centrifugation. The enriched neutrophil preparations were stimulated with N-formyl-methionyl-leucine-phenylalanine and phorbol myristic acid to induce the oxidative burst. It was found that neutrophils isolated from suspended animals released the same levels of superoxide anion as did vivarium control animals that were not suspended, indicating that whole body suspension did not alter this aspect of rat neutrophil function.

Non-NASA Center↗
NASA NTRS2022

Co-leveraging Scientific Advances in Space Biology and Astrobiology Towards Achieving NASA’s Life Science Objectives

Executive Summary: Distinct lines of scientific inquiry drives the separation of NASA’s fundamental life science research into Space Biology and Astrobiology. This division developed as a way to place life scientists alongside experts in the physical constraints that define the acclimation, adaptation and evolution of biology systems relevant to their respective subjects. For astrobiology, integration with disciplines such as geology, geochemistry, astronomy, planetary science, etc., enables a comprehensive assessment of the physical environment and its co-evolution with biological processes. Space Biology’s co-location with Physical Sciences places life science researchers adjacent to experts in the physical phenomena associated with microgravity and spaceflight, enabling an understanding of how the spaceflight environment affects biological systems. Despite this separation, aspects of both disciplines have converged on a similar, fundamental objective: to describe and understand the dynamics of complex living communities in the contexts of their physical environments. While the environmental systems and timescales are dramatically different, continuing to motivate the separation into distinct fields, similarities in the underlying objective present opportunities to find efficiencies, reduce overlap, and minimize duplication of effort. Space Biology and Astrobiology share a common need to understand microbial physiology in extreme environments – whether the ‘built’ spaceflight environment or the natural environments in which many astrobiology studies are conducted. In particular, open questions in each discipline require the development of quantitative frameworks, applicable at the ecosystem level, that support predictive capabilities for environments where observations are sparse. Additionally, both disciplines have a need to prepare, detect, and analyze the (potential) biological signal in complex samples-often in a completely autonomous fashion. The next decade will see NASA Space Biology moving to understand and describe the effects of the beyond low-earth orbit (BLEO) spaceflight environment on living systems. This new direction will dramatically reduce the opportunities for ground-based analysis of space-flown samples, driving space biology investigations towards fully autonomous experiments and missions. At the same time, astrobiology life detection missions aimed at detecting biosignatures on Mars and icy moons in the outer solar system could benefit from fully automated sample processing and analysis. There are opportunities to leverage instrument and method development between both disciplines within the context of these BLEO missions.

Astrobiology↗