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At least 505 records · Page 28

The tubulin structure, a quarter of a century later

This retrospective on the 25th anniversary of the publication of the first structure of tubulin is shaped by my own personal experiences rather than being a strict and complete historical account of the event. It is a reflection on how working in science felt many years ago, on the struggles and the joys of reaching for the high-hanging fruit, and, ultimately, on how relevant or not our personal scientific contributions are to the broader scientific community. Writing it brought back memories of my unique and sadly lost postdoctoral advisor Ken Downing, who dreamt of this structure and brought it to fruition against all odds.

59 BASIC BIOLOGICAL SCIENCES↗

Divergent selection and climate adaptation fuel genomic differentiation between sister species of Sphagnum (peat moss)

Abstract Background and Aims New plant species can evolve through the reinforcement of reproductive isolation via local adaptation along habitat gradients. Peat mosses (Sphagnaceae) are an emerging model system for the study of evolutionary genomics and have well-documented niche differentiation among species. Recent molecular studies have demonstrated that the globally distributed species Sphagnum magellanicum is a complex of morphologically cryptic lineages that are phylogenetically and ecologically distinct. Here, we describe the architecture of genomic differentiation between two sister species in this complex known from eastern North America: the northern S. diabolicum and the largely southern S. magniae. Methods We sampled plant populations from across a latitudinal gradient in eastern North America and performed whole genome and restriction-site associated DNA sequencing. These sequencing data were then analyzed computationally. Key Results Using sliding-window population genetic analyses we find that differentiation is concentrated within ‘islands’ of the genome spanning up to 400 kb that are characterized by elevated genetic divergence, suppressed recombination, reduced nucleotide diversity and increased rates of non-synonymous substitution. Sequence variants that are significantly associated with genetic structure and bioclimatic variables occur within genes that have functional enrichment for biological processes including abiotic stress response, photoperiodism and hormone-mediated signalling. Demographic modelling demonstrates that these two species diverged no more than 225 000 generations ago with secondary contact occurring where their ranges overlap. Conclusions We suggest that this heterogeneity of genomic differentiation is a result of linked selection and reflects the role of local adaptation to contrasting climatic zones in driving speciation. This research provides insight into the process of speciation in a group of ecologically important plants and strengthens our predictive understanding of how plant populations will respond as Earth’s climate rapidly changes.

58 GEOSCIENCES↗

Recent evolution of risk analyses in atomic bomb survivor studies: new methods and applications

Abstract Several decades ago a dramatic leap forward occurred in the development and application of statistical methods for modeling radiation risk at the Radiation Effects Research Foundation (RERF). Poisson regression analysis for grouped person-year cohort data and the linear excess relative risk model were introduced, and subsequently a devoted software system, Epicure® (https://www.hirosoft.com), was developed by researchers at RERF and at the U.S. National Cancer Institute. Numerous advancements in understanding radiation effects on humans were made possible with these methods, which are still the state-of-the-art for risk assessment at RERF and have remained part of the standard toolbox for radiation—and other environmental—epidemiological studies worldwide. Nevertheless, as our understanding of radiation risk has increased, so have the breadth and depth of questions that require answers based on emerging data that are not amenable to these conventional methods. This overview briefly recounts the conventional methods and then describes our recent diversification into the use or development of new statistical approaches to meet the challenges of burgeoning biological data and emerging mechanistic information. We briefly discuss the development and application of new methods, current and planned, that are part of the RERF Statistics Department’s role in supporting institution-wide research, especially in our collaborations involving the Life Span Study, Adult Health Study, and First-generation Offspring Clinical Study. Some approaches to modeling and assessing radiation risk with newer methods mentioned herein have already been published, while some are still in development or are only beginning at the proposal stage.

Oncology↗

Shared governance in the plant holobiont and implications for one health

Abstract The holobiont Holobiont theory is more than 80 years old, while the importance of microbial communities for plant holobionts was already identified by Lorenz Hiltner more than a century ago. Both concepts are strongly supported by results from the new field of microbiome research. Here, we present ecological and genetic features of the plant holobiont that underpin principles of a shared governance between hosts and microbes and summarize the relevance of plant holobionts in the context of global change. Moreover, we uncover knowledge gaps that arise when integrating plant holobionts in the broader perspective of the holobiome as well as one and planetary health concepts. Action is needed to consider interacting holobionts at the holobiome scale, for prediction and control of microbiome function to improve human and environmental health outcomes.

59 BASIC BIOLOGICAL SCIENCES↗

Phage display and other peptide display technologies

ABSTRACT Phage display technology, which is based on the presentation of peptide sequences on the surface of bacteriophage virions, was developed over 30 years ago. Improvements in phage display systems have allowed us to employ this method in numerous fields of biotechnology, as diverse as immunological and biomedical applications, the formation of novel materials and many others. The importance of phage display platforms was recognized by awarding the Nobel Prize in 2018 ‘for the phage display of peptides and antibodies’. In contrast to many review articles concerning specific applications of phage display systems published in recent years, we present an overview of this technology, including a comparison of various display systems, their advantages and disadvantages, and examples of applications in various fields of science, medicine and the broad sense of biotechnology. Other peptide display technologies, which employ bacterial, yeast and mammalian cells, as well as eukaryotic viruses and cell-free systems, are also discussed. These powerful methods are still being developed and improved; thus, novel sophisticated tools based on phage display and other peptide display systems are constantly emerging, and new opportunities to solve various scientific, medical and technological problems can be expected to become available in the near future.

Jaroszewicz, Weronika (ORCID:0000000302948682)↗

Comparative genome analyses suggest a hemibiotrophic lifestyle and virulence differences for the beech bark disease fungal pathogens Neonectria faginata and Neonectria coccinea

Abstract Neonectria faginata and Neonectria coccinea are the causal agents of the insect-fungus disease complex known as beech bark disease (BBD), known to cause mortality in beech forest stands in North America and Europe. These fungal species have been the focus of extensive ecological and disease management studies, yet less progress has been made toward generating genomic resources for both micro- and macro-evolutionary studies. Here, we report a 42.1 and 42.7 mb highly contiguous genome assemblies of N. faginata and N. coccinea, respectively, obtained using Illumina technology. These species share similar gene number counts (12,941 and 12,991) and percentages of predicted genes with assigned functional categories (64 and 65%). Approximately 32% of the predicted proteomes of both species are homologous to proteins involved in pathogenicity, yet N. coccinea shows a higher number of predicted mitogen-activated protein kinase genes, virulence determinants possibly contributing to differences in disease severity between N. faginata and N. coccinea. A wide range of genes encoding for carbohydrate-active enzymes capable of degradation of complex plant polysaccharides and a small number of predicted secretory effector proteins, secondary metabolite biosynthesis clusters and cytochrome oxidase P450 genes were also found. This arsenal of enzymes and effectors correlates with, and reflects, the hemibiotrophic lifestyle of these two fungal pathogens. Phylogenomic analysis and timetree estimations indicated that the N. faginata and N. coccinea species divergence may have occurred at ∼4.1 million years ago. Differences were also observed in the annotated mitochondrial genomes as they were found to be 81.7 kb (N. faginata) and 43.2 kb (N. coccinea) in size. The mitochondrial DNA expansion observed in N. faginata is attributed to the invasion of introns into diverse intra- and intergenic locations. These first draft genomes of N. faginata and N. coccinea serve as valuable tools to increase our understanding of basic genetics, evolutionary mechanisms and molecular physiology of these two nectriaceous plant pathogenic species.

Salgado-Salazar, Catalina↗

Worldwide Population Genomics Reveal Long-Term Stability of the Mitochondrial Genome Architecture in a Keystone Marine Plant

Mitochondrial genomes (mitogenomes) of flowering plants are composed of multiple chromosomes. Recombination within and between the mitochondrial chromosomes may generate diverse DNA molecules termed isoforms. The isoform copy number and composition can be dynamic within and among individual plants due to uneven replication and homologous recombination. Nonetheless, despite their functional importance, the level of mitogenome conservation within species remains understudied. Whether the ontogenetic variation translates to evolution of mitogenome composition over generations is currently unknown. Here we show that the mitogenome composition of the seagrass Zostera marina is conserved among worldwide populations that diverged ca. 350,000 years ago. Using long-read sequencing, we characterized the Z. marina mitochondrial genome and inferred the repertoire of recombination-induced configurations. To characterize the mitochondrial genome architecture worldwide and study its evolution, we examined the mitogenome in Z. marina meristematic region sampled in 16 populations from the Pacific and Atlantic oceans. Our results reveal a striking similarity in the isoform relative copy number, indicating a high conservation of the mitogenome composition among distantly related populations and within the plant germline, despite a notable variability during individual ontogenesis. Our study supplies a link between observations of dynamic mitogenomes at the level of plant individuals and long-term mitochondrial evolution.

(sub)stoichiometric shifting↗

Rapid Shifts in the Temperature Dependence of Locomotor Performance in an Invasive Frog, Xenopus laevis , Implications for Conservation

Abstract Temperature is a critical abiotic factor impacting all aspects of the biology of organisms, especially in ectotherms. As such, it is an important determinant of the potential invasive ability of organisms and may limit population expansion unless organisms can physiologically respond to changes in temperature either through plasticity or by adapting to their novel environment. Here, we studied the African clawed frog, Xenopus laevis, which has become invasive on a global scale. We compared adults from an invasive population of western France with individuals from two populations in the native range in South Africa. We measured the thermal dependence of locomotor performance in adults given its relevance to dispersal, predator escape, and prey capture. Our results show significant differences in the limits of the 80% performance breadth interval for endurance with the French population showing a left shift in its limits congruent with the colder climate experienced in France. The French invasive population was introduced only about 40 years ago suggesting a rapid shift in the thermal physiology. Given that all individuals were acclimated under laboratory conditions at 23°C for 2 months this suggests that the invasive frogs have adapted to their new environment. These data may allow the refinement of physiologically informed species distribution models permitting better estimates of future ranges at risk of invasion.

Araspin, Laurie↗

Tilted outer and inner structures in edge-on galaxies?

ABSTRACT Tilted and warped discs inside tilted dark matter haloes are predicted from numerical and semi-analytical studies. In this paper, we use deep imaging to demonstrate the likely existence of tilted outer structures in real galaxies. We consider two SB0 edge-on galaxies, NGC 4469 and NGC 4452, which exhibit apparent tilted outer discs with respect to the inner structure. In NGC 4469, this structure has a boxy shape, inclined by ΔPA ≈ 3° with respect to the inner disc, whereas NGC 4452 harbours a discy outer structure with ΔPA ≈ 6°. In spite of the different shapes, both structures have surface brightness profiles close to exponential and make a large contribution (∼30 per cent) to the total galaxy luminosity. In the case of NGC 4452, we propose that its tilted disc likely originates from a former fast tidal encounter (probably with IC 3381). For NGC 4469, a plausible explanation may also be galaxy harassment, which resulted in a tilted or even a tumbling dark matter halo. A less likely possibility is accretion of gas-rich satellites several Gyr ago. New deep observations may potentially reveal more such galaxies with tilted outer structures, especially in clusters. We also consider galaxies, mentioned in the literature, where a central component (a bar or a bulge) is tilted with respect to the stellar disc. According to our numerical simulations, one of the plausible explanations of such observed ‘tilts’ of the bulge/bar is a projection effect due to a not exactly edge-on orientation of the galaxy coupled with a skew angle of the triaxial bulge/bar.

Mosenkov, Aleksandr V.↗

SDSS-IV MaNGA: Bayesian analysis of the star formation history of low-mass galaxies in the local Universe

ABSTRACT We measure the star formation histories (SFHs) of a sample of low-mass galaxies with M* < 109 M⊙ from the SDSS-IV MaNGA survey. The large number of IFU spectra for each galaxy are either combined to reach a high signal to noise ratio or used to investigate spatial variations. We use Bayesian inferences based on full spectrum fitting. Our analysis based on Bayesian evidence ratio indicates a strong preference for a model that allows the presence of an old stellar population, and that an improper model for the SFH can significantly underestimate the old population in these galaxies. The addition of near-infrared photometry to the constraining data can further distinguish between different SFH model families and significantly tighten the constraints on the mass fraction in the old population. On average more than half of the stellar mass in present-day low-mass galaxies formed at least 8 Gyr ago, while about 30 per cent within the past 4 Gyr. Satellite galaxies on average have formed their stellar mass earlier than central galaxies. The radial dependence of the SFH is quite weak. Our results suggest that most of the low-mass galaxies have an early episode of active star formation that produces a large fraction of their present stellar mass.

Zhou, Shuang↗

The shape of SN 1993J re-analysed

SN 1993J is one of the best-studied Type IIb supernovae. Spectropolarimetric data analyses were published over two decades ago at a time when the field of supernova spectropolarimetry was in its infancy. Here, we present a new analysis of the spectropolarimetric data of SN 1993J and an improved estimate of its interstellar polarization (ISP) as well as a critical review of ISP removal techniques employed in the field. The polarization of SN 1993J is found to show significant alignment on the q - u plane, suggesting the presence of a dominant axis and therefore of continuum polarization. We also see strong line polarization features, including H β, He i λ5876, H α, He i λ6678, He i λ7065, and high velocity (HV) components of He i λ5876 and H α. SN 1993J is therefore the second example of a stripped-envelope supernova, alongside iPTF13bvn, with prominent HV helium polarization features, and the first to show a likely HV H α contribution. Overall, we determine that the observed features can be interpreted as the superposition of anisotropically distributed line forming regions over ellipsoidal ejecta. We cannot exclude the possibility of an off-axis energy source within the ejecta. These data demonstrate the rich structures that are inaccessible if solely considering the flux spectra but can be probed by spectropolarimetric observations. In future studies, the new ISP corrected data can be used in conjunction with 3D radiative transfer models to better map the geometry of the ejecta of SN 1993J.

79 ASTRONOMY AND ASTROPHYSICS↗

A panoramic view of the Local Group dwarf galaxy NGC 6822

ABSTRACT We present a panoramic survey of the isolated Local Group dwarf irregular galaxy NGC 6822. Our photometry reaches ∼2–3 mag deeper than most previous studies and spans the widest area around the dwarf compared to any prior work. We observe no stellar overdensities in the outskirts of NGC 6822 to V ∼ 30 mag arcsec−2 and a projected radius of 16.5 kpc. This indicates that NGC 6822 has not experienced any recent interaction with a companion galaxy, despite previous suggestions to the contrary. Similarly, we find no evidence for any dwarf satellites of NGC 6822 to a limiting luminosity MV ≈ −5. NGC 6822 contains a disc of H i gas and young stars, oriented at ∼60○ to an extended spheroid composed of old stellar populations. We observe no correlation between the distribution of young stars and spheroid members. Our imaging allows us to trace the spheroid to nearly 11 kpc along its major axis, commensurate with the extent of the NGC 6822 globular cluster system. We find that the spheroid becomes increasingly flattened at larger radii, and its position angle twists by up to 40○. We use Gaia EDR3 astrometry to measure a proper motion for NGC 6822, and then sample its orbital parameter space. While this galaxy has spent the majority of its life in isolation, we find that it likely passed within the virial radius of the Milky Way ∼3–4 Gyr ago. This may explain the apparent flattening and twisting observed in the outskirts of its spheroid.

79 ASTRONOMY AND ASTROPHYSICS↗

Stellar migration and chemical enrichment in the milky way disc: a hybrid model

ABSTRACT We develop a hybrid model of galactic chemical evolution that combines a multiring computation of chemical enrichment with a prescription for stellar migration and the vertical distribution of stellar populations informed by a cosmological hydrodynamic disc galaxy simulation. Our fiducial model adopts empirically motivated forms of the star formation law and star formation history, with a gradient in outflow mass loading tuned to reproduce the observed metallicity gradient. With this approach, the model reproduces many of the striking qualitative features of the Milky Way disc’s abundance structure: (i) the dependence of the [O/Fe]–[Fe/H] distribution on radius Rgal and mid-plane distance |z|; (ii) the changing shapes of the [O/H] and [Fe/H] distributions with Rgal and |z|; (iii) a broad distribution of [O/Fe] at sub-solar metallicity and changes in the [O/Fe] distribution with Rgal, |z|, and [Fe/H]; (iv) a tight correlation between [O/Fe] and stellar age for [O/Fe] > 0.1; (v) a population of young and intermediate-age α-enhanced stars caused by migration-induced variability in the Type Ia supernova rate; (vi) non-monotonic age–[O/H] and age–[Fe/H] relations, with large scatter and a median age of ∼4 Gyr near solar metallicity. Observationally motivated models with an enhanced star formation rate ∼2 Gyr ago improve agreement with the observed age–[Fe/H] and age–[O/H] relations, but worsen agreement with the observed age–[O/Fe] relation. None of our models predict an [O/Fe] distribution with the distinct bimodality seen in the observations, suggesting that more dramatic evolutionary pathways are required. All code and tables used for our models are publicly available through the Versatile Integrator for Chemical Evolution (VICE; https://pypi.org/project/vice).

79 ASTRONOMY AND ASTROPHYSICS↗

Using action space clustering to constrain the recent accretion history of Milky Way-like galaxies

ABSTRACT In the currently favoured cosmological paradigm galaxies form hierarchically through the accretion of satellites. Since a satellite is less massive than the host, its stars occupy a smaller volume in action space. Actions are conserved when the potential of the host halo changes adiabatically, so stars from an accreted satellite would remain clustered in action space as the host evolves. In this paper, we identify recently disrupted accreted satellites in three Milky Way-like disc galaxies from the cosmological baryonic FIRE-2 simulations by tracking satellites through simulation snapshots. We try to recover these satellites by applying the cluster analysis algorithm Enlink to the orbital actions of accreted star particles in the z = 0 snapshot. Even with completely error-free mock data we find that only 35 per cent (14/39) satellites are well recovered while the rest (25/39) are poorly recovered (i.e. either contaminated or split up). Most (10/14 ∼70 per cent) of the well-recovered satellites have infall times <7.1 Gyr ago and total mass >4 × 108M⊙ (stellar mass more than 1.2 × 106 M⊙, although our upper mass limit is likely to be resolution dependent). Since cosmological simulations predict that stellar haloes include a population of in situ stars, we test our ability to recover satellites when the data include 10–50 per cent in situ contamination. We find that most previously well-recovered satellites stay well recovered even with 50 per cent contamination. With the wealth of 6D phase space data becoming available we expect that cluster analysis in action space will be useful in identifying the majority of recently accreted and moderately massive satellites in the Milky Way.

79 ASTRONOMY AND ASTROPHYSICS↗

Quenching time-scales in the IllustrisTNG simulation

ABSTRACT The time-scales for galaxy quenching offer clues to its underlying physical drivers. We investigate central galaxy quenching time-scales in the IllustrisTNG 100-1 simulation, their evolution over time, and the pre-quenching properties of galaxies that predict their quenching time-scales. Defining quenching duration τq as the time between crossing specific star formation rate (sSFR) thresholds, we find that ${\sim} 40{{\ \rm per\ cent}}$ of galaxies quench rapidly with τq < 1 Gyr, but a substantial tail of galaxies can take up to 10 Gyr to quench. Furthermore, 29 per cent of galaxies that left the star-forming main sequence (SFMS) more than 2 Gyr ago never fully quench by z = 0. While the median τq is fairly constant with epoch, the rate of galaxies leaving the SFMS increases steadily over cosmic time, with the rate of slow quenchers being dominant around z ∼ 2–0.7. Compared to fast quenchers (τq < 1 Gyr), slow-quenching galaxies (τq > 1 Gyr) were more massive, had more massive black holes, had larger stellar radii, and accreted gas with higher specific angular momentum (AM) prior to quenching. These properties evolve little by z = 0, except for the accreting gas AM for fast quenchers, which reaches the same high AM as the gas in slow quenchers. By z = 0, slow quenchers also have residual star formation in extended gas rings. Using the expected relationship between stellar age gradient and τq for inside-out quenching we find agreement with Mapping Nearby Galaxies at Apache Point Observatory (MaNGA) integral field unit (IFU) observations. Our results suggest the accreting gas AM and potential well depth determine the quenching time-scale.

79 ASTRONOMY AND ASTROPHYSICS↗

A tale of a tail: a tidally disrupting ultra-diffuse galaxy in the M81 group

ABSTRACT We present the discovery of a giant tidal tail of stars associated with F8D1, the closest known example of an ultra-diffuse galaxy (UDG). F8D1 sits in a region of the sky heavily contaminated by Galactic cirrus and has been poorly studied since its discovery two decades ago. The tidal feature was revealed in a deep map of resolved red giant branch stars constructed using data from our Subaru Hyper Suprime-Cam survey of the M81 Group. It has an average surface brightness of μg ∼ 32 mag arcsec−2 and can be traced for over a degree on the sky (60 kpc at the distance of F8D1) with our current imagery. We revisit the main body properties of F8D1 using deep multiband imagery acquired with MegaCam on CFHT and measure effective radii of 1.7–1.9 kpc, central surface brightnesses of 24.7–25.7 mag, and a stellar mass of ∼7 × 107M⊙. Assuming a symmetric feature on the other side of the galaxy, we calculate that 30–36 per cent of F8D1’s present-day luminosity is contained in the tail. We argue that the most likely origin of F8D1’s disruption is a recent close passage to M81, which would have stripped its gas and quenched its star formation. As the only UDG that has so far been studied to such faint surface brightness depths, the unveiling of F8D1’s tidal disruption is important. It leaves open the possibility that many other UDGs could be the result of similar processes, with the most telling signatures of this lurking below current detection limits.

Žemaitis, Rokas (ORCID:0000000285660491)↗

Spectral study of star-forming rings in S0 galaxies of Dorado group – NGC 1533 and NGC 1543

ABSTRACT We have fulfilled a detailed long-slit spectroscopic analysis for two SB0 galaxies – NGC 1533 and NGC 1543, – belonging to the Dorado group. Our spectral data reveal asymmetric decoupled kinematics of the stars and ionized gas in these barred lenticular galaxies that give evidences for external origin of the gas in the rings. We have calculated the star formation rates in the rings by using the ultraviolet fluxes of the rings corrected for the foreground and intrinsic absorption; and we have estimated parameters of the stellar populations in the inner parts of the galaxies confirming that they are old – except the nucleus of NGC 1543, which demonstrates signatures of rejuvenation less than 5 Gyr ago.

Proshina, Irina S.↗

Understanding the origin of early-type dwarfs: the spectrophotometric study of CGCG014−074

ABSTRACT Early-type dwarf galaxies constitute a prevalent population in the central regions of rich groups and clusters in the local Universe. These low-luminosity and low-mass stellar systems play a fundamental role in the assembly of the luminous galaxies observed today, according to the Lambda cold dark matter hierarchical theory. The origin of early-type dwarfs has been linked to the transformation of disc galaxies interacting with the intracluster medium, especially in dense environments. However, the existence of low-luminosity early-type galaxies in low-density environments presents a challenge to this scenario. This study presents a comprehensive photometric and spectroscopic analysis of the early-type dwarf galaxy CGCG014−074 using deep Gemini GMOS (Gemini Multi-Object Spectrograph) data, focusing on its peculiarities and evolutionary implications. CGCG014−074 exhibits distinct features, including a rotating inner disc, an extended stellar formation with a quiescent phase since about 2 Gyr ago, and the presence of boxy isophotes. From the kinematic analysis, we confirm CGCG014−074 as a nucleated early-type dwarf galaxy with embedded disc. The study of its stellar population parameters using different methods provides significant insights into the galaxy’s evolutionary history. These results show an old and metal-poor nucleus (${\sim}9.3$ Gyr and $\mathrm{[Z/H]}\sim -0.84$ dex), while the stellar disc is younger (${\sim}4.4$ Gyr) with a higher metallicity ($\mathrm{[Z/H]}\sim -0.40$ dex). These distinctive features collectively position CGCG014−074 as a likely building block galaxy that has evolved passively throughout its history.

Astronomy & Astrophysics↗