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NASA NTRS1994

Permeability of membranes to amino acids and modified amino acids: mechanisms involved in translocation

The amino acid permeability of membranes is of interest because they are one of the key solutes involved in cell function. Membrane permeability coefficients (P) for amino acid classes, including neutral, polar, hydrophobic, and charged species, have been measured and compared using a variety of techniques. Decreasing lipid chain length increased permeability slightly (5-fold), while variations in pH had only minor effects on the permeability coefficients of the amino acids tested in liposomes. Increasing the membrane surface charge increased the permeability of amino acids of the opposite charge, while increasing the cholesterol content decreased membrane permeability. The permeability coefficients for most amino acids tested were surprisingly similar to those previously measured for monovalent cations such as sodium and potassium (approximately 10(-12)-10(-13) cm s-1). This observation suggests that the permeation rates for the neutral, polar and charged amino acids are controlled by bilayer fluctuations and transient defects, rather than partition coefficients and Born energy barriers. Hydrophobic amino acids were 10(2) more permeable than the hydrophilic forms, reflecting their increased partition coefficient values. External pH had dramatic effects on the permeation rates for the modified amino acid lysine methyl ester in response to transmembrane pH gradients. It was established that lysine methyl ester and other modified short peptides permeate rapidly (P = 10(-2) cm s-1) as neutral (deprotonated) molecules. It was also shown that charge distributions dramatically alter permeation rates for modified di-peptides. These results may relate to the movement of peptides through membranes during protein translocation and to the origin of cellular membrane transport on the early Earth.

Review↗

NASA NTRS2012

An Overview of Scientific and Space Weather Results from the Communication/Navigation Outage Forecasting System (C/NOFS) Mission

The Communication/Navigation Outage Forecasting System (C/NOFS) Mission of the Air Force Research Laboratory is described. C/NOFS science objectives may be organized into three categories: (1) to understand physical processes active in the background ionosphere and thermosphere in which plasma instabilities grow; (2) to identify mechanisms that trigger or quench the plasma irregularities responsible for signal degradation; and (3) to determine how the plasma irregularities affect the propagation of electromagnetic waves. The satellite was launched in April, 2008 into a low inclination (13 deg), elliptical (400 x 850 km) orbit. The satellite sensors measure the following parameters in situ: ambient and fluctuating electron densities, AC and DC electric and magnetic fields, ion drifts and large scale ion composition, ion and electron temperatures, and neutral winds. C/NOFS is also equipped with a GPS occultation receiver and a radio beacon. In addition to the satellite sensors, complementary ground-based measurements, theory, and advanced modeling techniques are also important parts of the mission. We report scientific and space weather highlights of the mission after nearly four years in orbit

Pfaff, R.↗

NASA NTRS1992

Hydrogen leak detection in the Space Shuttle

This study focuses on a helium gas jet flowing into room air. Measurements of helium concentration and velocity in the jet-air mixture are reported. The objective is to learn about jet characteristics so that dynamically similar hydrogen leaks may be located in the Space Shuttle. The hazardous gas detection system (HGDS) in the mobile launch pad uses mass spectrometers to monitor the shuttle environment for leaks. The mass spectrometers are fed by long sample tubes which draw gas from the payload bay, mid body, aft engine compartment and external tank. The overall purpose of this study is to improve the HGDS especially in its potential for locating hydrogen leaks. A rapid-response leak detection experiment was designed, built, and tested, following on the work done in this program last summer. The apparatus included a Perkin Elmer MGA-1200 mass spectrometer and air velocity transducer, both monitored by a Macintosh IIFX computer using LabVIEW software. A jet of helium flowing into the lab air simulated a gas leak. Steady helium or hydrogen-nitrogen jets were logged for concentration and velocity, and the power spectral density of each was computed. Last year, large eddies and vortices were visually seen with Schlieren imaging, and they were detected in the time plots of the various instruments. The response time of the MGA-1200 was found in the range of 0.05 to 0.1 sec. Pulsed concentration waves were clearly detected at 25 cycles per sec by spectral analysis of MGA data. No peaks were detected in the power spectrum, so in the present study, 10 Hz bandwidth-averaged power levels were examined at regular frequency intervals. The practical consequences of last year's study are as follows: sampling frequency should be increased above the present rate of 1 sample per second so that transients could be observed and analyzed with frequency response methods. Many more experiments and conditions were observed in this second summer, including the effects of orifice diameter, jet velocity, sample tube design, radial effects, vertical flow, and low hydrogen concentrations (1 percent). A frequent observation was that the power spectrum, calculated from the Fourier transform of concentration fluctuations, gives a separate piece of information from concentration. Many of the tests suggest that power is high where mixing occurs at the helium-air interface. This fact is apparently independent of the concentration level, which could be high or low, but depends on the sample location relative to the jet (leak) origin, whereas high concentration may be due to a strong leak far away or a small leak close to the sample tube. If the power is low for any concentration level, this would signify helium is arriving at the sample tube by diffusion, not chaotic mixing caused by the jet interaction with air. The practical result is to propose a modification of the HGDL mass spectrometer data sampling and software so that sampling rates could be capable of observing at least 25 Hz fluctuations.

Barile, Ronald G↗

NASA NTRS2006

Ultra-fine-scale filamentary structures in the Outer Corona and the Solar Magnetic Field

Filamentary structures following magnetic field lines pervade the Sun's atmosphere and offer us insight into the solar magnetic field. Radio propagation measurements have shown that the smallest filamentary structures in the solar corona are more than 2 orders of magnitude finer than those seen in solar imaging. Here we use radio Doppler measurements to characterize their transverse density gradient and determine their finest scale in the outer corona at 20-30 R(circled dot operator), where open magnetic fields prevail. Filamentary structures overly active regions have the steepest gradient and finest scale, while those overlying coronal holes have the shallowest gradient and least finest scale. Their organization by the underlying corona implies that these subresolution structures extend radially from the entire Sun, confirming that they trace the coronal magnetic field responsible for the radial expansion of the solar wind. That they are rooted all over the Sun elucidates the association between the magnetic field of the photosphere and that of the corona, as revealed by the similarity between the power spectra of the photospheric field and the coronal density fluctuations. This association along with the persistence of filamentary structures far from the Sun demonstrate that subresolution magnetic fields must play an important role not only in magnetic coupling of the photosphere and corona, but also in coronal heating and solar wind acceleration through the process of small-scale magnetic reconnection. They also explain why current widely used theoretical models that extrapolate photospheric magnetic fields into the corona do not predict the correct source of the solar wind.

Sun : Corona↗

NASA NTRS2016

Influence of Molecular Simulation Model Accuracy on the Interfacial Properties of an Ionic Liquid: Overview of Recommended Practices

Increasing the energy storage capability of ionic liquid supercapacitors will require better understanding of ion-electrode interactions. We have probed the influence of these interactions on the structure and differential capacitance of of an ionic liquid ([EMIM][BF4]) at an ideal graphite interface as a function of model accuracy. Of note, differential capacitance is determined through newly derived and validated fluctuation formulas. In terms of model accuracy, we test electrostatic techniques, electrode charging techniques, and electrolyte interatomic potentials. For electrostatic summations, we employ high cost, high fidelity techniques as well as less expensive, approximate techniques for summation in slab geometry. For electrode charging, uniform, constant-charge and environmentally responsive, constant-potential conditions are employed. For the ionic liquid, constant charge and atomically polarizable models are employed. We comment on the role of model accuracy on the structure and energetics of the electric double layer as well as on the magnitude and shape of differential capacitance.

simulations↗

NASA NTRS2023

Structured Type III Radio Bursts Observed in Interplanetary Space

Context . The last few decades have seen numerous studies dedicated to fine structures of type III radio bursts observed in the meter–decameter wavelengths. Most of the explanations of the structured radio emission involve the propagation of electron beams through the strongly inhomogeneous plasma in the low corona. To date, only a few type III bursts with fine structures, observed at hecto-kilometric wavelengths, have been reported. Aims . We report here the existence of numerous structured type III radio bursts observed during the STEREO era by all three WAVES instruments on board STEREO A, B, and Wind. The aim of the study is to report and classify structured type III bursts, and to present the characteristics of their fine structures. The final goal is to try to understand the physical mechanism responsible for the generation of structured radio emission. Methods . In this study we used data from all available spacecraft, specifically STEREO and Wind. We employed 1D density models to obtain the speed of the source of type III radio emission, the electron beam. We also performed a spectral analysis of the fine structures in order to compare their characteristics with the metric–decametric fine structures. Results . The presented similarities of the type III fine structures in the metric to decametric and interplanetary wavelengths indicate that the physical processes responsible for the generation of structured type III radio bursts could be the same, at heights from the low corona to the interplanetary range. We show that the observed structuring and intermittent nature of the type III bursts can be explained by the variation in the level of density fluctuations, at different distances from the Sun.

Non-thermal radiation mechanisms↗

NASA NTRS2006

Ice Mass Fluctuations and Earthquake Hazard

In south central Alaska, tectonic strain rates are high in a region that includes large glaciers undergoing ice wastage over the last 100-150 years [Sauber et al., 2000; Sauber and Molnia, 2004]. In this study we focus on the region referred to as the Yakataga segment of the Pacific-North American plate boundary zone in Alaska. In this region, the Bering and Malaspina glacier ablation zones have average ice elevation decreases from 1-3 meters/year (see summary and references in Molnia, 2005). The elastic response of the solid Earth to this ice mass decrease alone would cause several mm/yr of horizontal motion and uplift rates of up to 10-12 mm/yr. In this same region observed horizontal rates of tectonic deformation range from 10 to 40 mm/yr to the north-northwest and the predicted tectonic uplift rates range from -2 mm/year near the Gulf of Alaska coast to 12mm/year further inland [Savage and Lisowski, 1988; Ma et al, 1990; Sauber et al., 1997, 2000, 2004; Elliot et al., 2005]. The large ice mass changes associated with glacial wastage and surges perturb the tectonic rate of deformation at a variety of temporal and spatial scales. The associated incremental stress change may enhance or inhibit earthquake occurrence. We report recent (seasonal to decadal) ice elevation changes derived from data from NASA's ICESat satellite laser altimeter combined with earlier DEM's as a reference surface to illustrate the characteristics of short-term ice elevation changes [Sauber et al., 2005, Muskett et al., 2005]. Since we are interested in evaluating the effect of ice changes on faulting potential, we calculated the predicted surface displacement changes and incremental stresses over a specified time interval and calculated the change in the fault stability margin using the approach given by Wu and Hasegawa [1996]. Additionally, we explored the possibility that these ice mass fluctuations altered the seismic rate of background seismicity. Although we primarily focus on evaluating the influence of ice mass changes since the end of the little Ice Age, the study is partially motivated by paleoseismic evidence from Yakataga and Kodiak regions which suggests that earlier glacier retreat may be associated with large earthquakes [Sauber et al., 2000; Carver et al., 2003].

Sauber, J.↗

NASA NTRS1974

Surface pressure fluctuations in hypersonic turbulent boundary layers

The surface pressure fluctuations on a flat plate model at hypersonic Mach numbers of 5.2, 7.4 and 10.4 with an attached turbulent boundary layer were measured using flush mounted small piezoelectric sensors. A high frequency resolution of the pressure field was achieved using specially designed small piezoelectric sensors that had a good frequency response well above 300 KHz. The RMS pressures and non-dimensional energy spectra for all above Mach numbers are presented. The convective velocities, obtained from space time correlation considerations are equal to 0.7 U sub infinity. The results indicate the RMS pressures vary from 5 to 25 percent of the mean static pressures. The ratios of RMS pressure to dynamic pressure are less than the universally accepted subsonic value of 6 x 10/3. The ratio decreases in value as the Mach number or the dynamic pressure is increased. The ratio of RMS pressure to wall shear for Mach number 7.4 satisfies one smaller than or equal to p/tau sub w smaller than or equal to three.

Raman, K. R.↗

NASA NTRS1980

Infrared heterodyne receivers with IF responses approaching 5 GHz

Specialized coherent 10.6 micrometer infrared receivers were developed with infrared frequency responses approaching 5 GHz. Carbon dioxide lasers were employed for these applications because of their availability, stability, and high average power levels. Heterodyne receivers which employ extrinsic photoconductive Ge:Cu(Sb) mixers were developed for measurements of carbon dioxide laser scattering to determine: (1) the drive lower hybrid wave density fluctuations; and (2) the plasma ion temperature of the dense Tokamak plasmas.

Wolczok, J. M.↗

NASA NTRS1984

A study of dynamic measurements made in the settling chamber of the Langley 0.3-meter transonic cryogenic tunnel

Tests have been conducted in a cryogenic wind tunnel settling chamber using a fast response instrumentation to measure the possible existence of temperature fronts due to a sudden or step change in the rate of liquid nitrogen injection into the circuit. No indications of such fronts were obtained using three different techniques to change the rate of nitrogen injection. The normalized pressure and velocity fluctuations at two total temperatures and over a large range of Mach numbers and Reynolds numbers were about 2 x 10 to the -7th to 2 x 10 to the -6th and about 1.8 to 3 percent, respectively. There was no evidence of liquid nitrogen droplets in the flow down to a total temperature of 140 K. The pressure fluctuation power spectra from a pressure transducer correlated with the fan blade passage through the eighth harmonic of the fundamental frequency.

Johnson, C. B.↗

NASA NTRS1989

How robust are distributed systems

A distributed system is made up of large numbers of components operating asynchronously from one another and hence with imcomplete and inaccurate views of one another's state. Load fluctuations are common as new tasks arrive and active tasks terminate. Jointly, these aspects make it nearly impossible to arrive at detailed predictions for a system's behavior. It is important to the successful use of distributed systems in situations in which humans cannot provide the sorts of predictable realtime responsiveness of a computer, that the system be robust. The technology of today can too easily be affected by worn programs or by seemingly trivial mechanisms that, for example, can trigger stock market disasters. Inventors of a technology have an obligation to overcome flaws that can exact a human cost. A set of principles for guiding solutions to distributed computing problems is presented.

Birman, Kenneth P.↗

NASA NTRS1987

Force, mass, and energy budgets of the Crary Ice Rise complex, Antarctica

The stress, mass, and energy-dissipation budgets of Crary Ice Rise are analyzed using field data collected during the 1983-1985 austral summers and in previous field programs. In addition, the net back pressure and ice-discharge rate along the grounding lines of ice streams are calculated to assess the effect of the ice rise on the surrounding flow. Comparison of the ice-rise budgets with the analysis of grounding-line data confirms the influence of the ice rise on ice-sheet stability, and suggests that Crary Ice Rise may have formed recently in response to an acceleration of one of the ice streams. It is concluded that feedback between ice-stream acceleration and ice-rise formation may control the future evolution of the above ice stream and promote long-term grounding-line stability in the face of strong natural fluctuations.

Macayeal, D. R.↗

NASA NTRS1981

The electrical conductivity of the Earth's upper mantle as estimated from satellite measured magnetic field variations

Low latitude magnetic field variations (magnetic storms) caused by large fluctuations in the equatorial ring current were derived from magnetic field magnitude data obtained by OGO 2, 4, and 6 satellites over an almost 5 year period. Analysis procedures consisted of (1) separating the disturbance field into internal and external parts relative to the surface of the Earth; (2) estimating the response function which related to the internally generated magnetic field variations to the external variations due to the ring current; and (3) interpreting the estimated response function using theoretical response functions for known conductivity profiles. Special consideration is given to possible ocean effects. A temperature profile is proposed using conductivity temperature data for single crystal olivine. The resulting temperature profile is reasonable for depths below 150-200 km, but is too high for shallower depths. Apparently, conductivity is not controlled solely by olivine at shallow depths.

Didwall, E. M.↗

NASA NTRS1992

Near-wall response in turbulent shear flows subjected to imposed unsteadiness

Rapid-distortion theory is adapted to introduce a truly unsteady closure into a simple phenomenological turbulence model in order to describe the unsteady response of a turbulent wall layer exposed to a temporarily oscillating pressure gradient. The closure model is built by taking the ratio of turbulent shear stress to turbulent kinetic energy to be a function of the effective strain. The latter accounts for the history of the flow. The computed unsteady velocity fluctuations and modulated turbulent stresses compare favorably in the 'non-quasi-steady' frequency range, where quasi-steady assumptions would fail. This suggests that the concept of rapid distortion is especially appropriate for unsteady flows. This paper forms the basis for acoustical studies of the problem to be reported elsewhere.

Mankbadi, Reda R.↗

NASA NTRS1992

Time dependent heat transfer rates in high Reynolds number hypersonic flowfields

Time dependent heat transfer rates have been calculated from time dependent temperature measurements in the vicinity of shock-wave boundary-layer interactions due to conical compression ramps on an axisymmetric body. The basic model is a cylindrical body with a 10 degree conical nose. Four conical ramps, 20, 25, 30, and 35 degrees serve as shock wave generators. Flowfield surveys have been made in the vicinity of the conical ramp vertex, the separation point, and the reattachment point. A significant effort was made to characterize the natural frequencies and relative powers of the resulting fluctuations in heat transfer rates. This research effort, sponsored jointly by NASA and the Air Force, was conducted in the Air Force Flight Dynamics Directorate High Reynolds Facility. The nominal freestream Mach number was 6, and the freestream Reynolds numbers ranged from 2.2 million/ft to 30.0 million/ft. Experimental results quantify temperature response and the resulting heat transfer rates as a function of ramp angle and Reynolds number. The temperature response within the flowfield appears to be steady-state for all compression ramp angles and all Reynolds numbers, and hence, the heat transfer rates appear to be steady-state.

Flanagan, Michael J.↗

NASA NTRS2003

An Experimental Investigation of Unsteady Surface Pressure on an Airfoil in Turbulence

Measurements of fluctuating surface pressure were made on a NACA 0015 airfoil immersed in grid generated turbulence. The airfoil model has a 2 ft chord and spans the 6 ft Virginia Tech Stability Wind Tunnel test section. Two grids were used to investigate the effects of turbulence length scale on the surface pressure response. A large grid which produced turbulence with an integral scale 13% of the chord and a smaller grid which produced turbulence with an integral scale 1.3% of the chord. Measurements were performed at angles of attack, alpha from 0 to 20 . An array of microphones mounted subsurface was used to measure the unsteady surface pressure. The goal of this measurement was to characterize the effects of angle of attack on the inviscid response. Lift spectra calculated from pressure measurements at each angle of attack revealed two distinct interaction regions; for omega(sub r) = omega b / U(sub infinity) is less than 10 a reduction in unsteady lift of up to 7 decibels (dB) occurs while an increase occurs for omega(sub r) is greater than 10 as the angle of attack is increased. The reduction in unsteady lift at low omega(sub r) with increasing angle of attack is a result that has never before been shown either experimentally or theoretically. The source of the reduction in lift spectral level appears to be closely related to the distortion of inflow turbulence based on analysis of surface pressure spanwise correlation length scales. Furthermore, while the distortion of the inflow appears to be critical in this experiment, this effect does not seem to be significant in larger integral scale (relative to the chord) flows based on the previous experimental work of McKeough suggesting the airfoils size relative to the inflow integral scale is critical in defining how the airfoil will respond under variation of angle of attack. A prediction scheme is developed that correctly accounts for the effects of distortion when the inflow integral scale is small relative to the airfoil chord. This scheme utilizes Rapid Distortion Theory to account for the distortion of the inflow with the distortion field modeled using a circular cylinder.

Mish, Patrick F.↗

NASA NTRS1992

The maintenance of low-frequency atmospheric anomalies

A linear diagnostic analysis of four prominent low-frequency atmospheric perturbation patterns in a GCM is carried out to determine the mechanism responsible for the maintenance of these patterns. Results indicate that interactions between the low-frequency patterns and the time-mean zonal asymmetries of the model climate are crucial to the maintenance of the patterns. Of equal importance are anomalous fluxes from transients. Vorticity fluxes due to bandpass (1-7 days) fluctuations ranging over broad sectors of the globe were found to be the most important components of the maintenance of flux anomalies. The nonlinear interactions of the low-frequency deviations with themselves were only of secondary importance.

Branstator, Grant↗

NASA NTRS1995

Small scales formation via Alfven wave propagation in compressible nonuniform media

In weakly dissipative media governed by the magnetohydrodynamics (MHD) equations, any efficient mechanism of energy dissipation requires the formation of small scales. The possibility to produce small scales has been studied by Malara et al. in the case of MHD disturbances propagating in an incompressible and inhomogeneous medium, for a strictly 2D geometry. We extend the work of Malara et al. to include both compressibility and the third component for vector quantities. Using numerical simulations we show that, when an Alfven wave propagates in a compressible nonuniform medium, the two dynamical effects responsible for the small scales formation in the incompressible case are still at work: energy pinching and phase-mixing. Moreover, the interaction between the initial Alfven wave and the inhomogeneity gives rise to the formation of compressible perturbations (fast and slow waves or a static entropy wave). Some of these compressive fluctuations are subject to the steepening of the wave front and become shock waves, which are extremely efficient in dissipating their energy, their dissipation being independent of the Reynolds number. A rough estimate of the typical times which the various dynamical processes take to produce small scales and then to dissipate the energy show that these times are consistent with those required to dissipate inside the solar corona the energy of Alfven waves of photospheric origin.

Malara, F.↗