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At least 487 records · Page 27

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DOE OSTI2022

Biosensor for branched-chain amino acid metabolism in yeast and applications in isobutanol and isopentanol production

Abstract Branched-chain amino acid (BCAA) metabolism fulfills numerous physiological roles and can be harnessed to produce valuable chemicals. However, the lack of eukaryotic biosensors specific for BCAA-derived products has limited the ability to develop high-throughput screens for strain engineering and metabolic studies. Here, we harness the transcriptional regulator Leu3p from Saccharomyces cerevisiae to develop a genetically encoded biosensor for BCAA metabolism. In one configuration, we use the biosensor to monitor yeast production of isobutanol, an alcohol derived from valine degradation. Small modifications allow us to redeploy Leu3p in another biosensor configuration that monitors production of the leucine-derived alcohol, isopentanol. These biosensor configurations are effective at isolating high-producing strains and identifying enzymes with enhanced activity from screens for branched-chain higher alcohol (BCHA) biosynthesis in mitochondria as well as cytosol. Furthermore, this biosensor has the potential to assist in metabolic studies involving BCAA pathways, and offers a blueprint to develop biosensors for other products derived from BCAA metabolism.

59 BASIC BIOLOGICAL SCIENCES↗

NASA NTRS2015

Ongoing Fixed Wing Research within the NASA Langley Aeroelasticity Branch

The NASA Langley Aeroelasticity Branch is involved in a number of research programs related to fixed wing aeroelasticity and aeroservoelasticity. These ongoing efforts are summarized here, and include aeroelastic tailoring of subsonic transport wing structures, experimental and numerical assessment of truss-braced wing flutter and limit cycle oscillations, and numerical modeling of high speed civil transport configurations. Efforts devoted to verification, validation, and uncertainty quantification of aeroelastic physics in a workshop setting are also discussed. The feasibility of certain future civil transport configurations will depend on the ability to understand and control complex aeroelastic phenomena, a goal that the Aeroelasticity Branch is well-positioned to contribute through these programs.

Bartels, Robert↗

DOE OSTI2026

A primer on forest structure measurement with lidar for ecologists

Light detection and ranging (lidar) technology has fundamentally advanced the way we measure forest structure, facilitating new insights into ecological processes. Lidar for forest ecology applications is deployed on multiple types of platforms that operate from the ground, air, or space, and each has associated strengths and limitations. Ideally, the choice of what kind of lidar to use in a particular study should be guided by the ecological question of interest; however, practical considerations of cost, data availability, and processing tools can be equally important. This synthesis is a practical introduction to how different lidar platforms characterize forest structure (e.g., tree size/location, wood volume, branching structure, aboveground biomass, leaf properties), designed for a general audience of ecologists (not remote sensing scientists) seeking an accessible introduction to the use of lidar. We also provide examples of novel ecological insights from recent lidar research and describe current limitations and areas of expected improvement. Last, we include an appendix of data collected from terrestrial, mobile, unoccupied aerial system, airplane, and satellite lidar platforms within a common temperate forest area, with associated code to allow new lidar users to visualize and manipulate data in R.

Cushman, KC [ORNL] (ORCID:0000000234641151)↗

DOE OSTI2020

Majorana-based quantum computing in nanowire devices

The boundary of one-dimensional topological superconductors might lead to the appearance of Majorana zero modes, whose nontrivial exchange statistics can be used for quantum computing. In branched nanowire networks, one can exchange Majorana modes by time dependently tuning topologically nontrivial parameter regions. In this paper, we simulate the exchange of four Majorana modes in T-shaped junctions made out of $\textit{p}$-wave superconducting Rashba wires. Specifically, we derive concrete experimental predictions for (quasi-)adiabatic braiding times and determine geometric conditions for successful Majorana exchange processes. Moreover, we prove that, in the adiabatic limit, the gating time needs to be smaller than the inverse of the squared superconducting order parameter and scales linearly with the gating potential. Furthermore, we show how to circumvent the formation of additional Majorana modes in branched nanowire systems, arising at wire intersection points of narrow junctions. Finally, we propose a multiqubit setup, allowing for universal quantum computing.

75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND↗

DOE OSTI2024

Search for the e + e − → ϕ χ c 1 ( 3872 ) process at BESIII

Based on 368.5 p b − 1 of e + e − collision data collected at center-of-mass energies 4.914 and 4.946 GeV by the BESIII detector, the e + e − → ϕ χ c 1 ( 3872 ) process is searched for the first time. No significant signal is observed and the upper limits at the 90% confidence level on the product of the Born cross section σ ( e + e − → ϕ χ c 1 ( 3872 ) ) and the branching fraction B [ χ c 1 ( 3872 ) → π + π − J / ψ ] at 4.914 and 4.946 GeV are set to be 0.85 and 0.96 pb, respectively. These measurements provide useful information for the production of the χ c 1 ( 3872 ) at e + e − colliders and deepen our understanding about the nature of this particle. Published by the American Physical Society 2024

Astronomy & Astrophysics↗

DOE OSTI2020

Functional and genetic markers of niche partitioning among enigmatic members of the human oral microbiome

Abstract Introduction Microbial residents of the human oral cavity have long been a major focus of microbiology due to their influence on host health and intriguing patterns of site specificity amidst the lack of dispersal limitation. However, the determinants of niche partitioning in this habitat are yet to be fully understood, especially among taxa that belong to recently discovered branches of microbial life. Results Here, we assemble metagenomes from tongue and dental plaque samples from multiple individuals and reconstruct 790 non-redundant genomes, 43 of which resolve to TM7, a member of the Candidate Phyla Radiation, forming six monophyletic clades that distinctly associate with either plaque or tongue. Both pangenomic and phylogenomic analyses group tongue-specific clades with other host-associated TM7 genomes. In contrast, plaque-specific TM7 group with environmental TM7 genomes. Besides offering deeper insights into the ecology, evolution, and mobilome of cryptic members of the oral microbiome, our study reveals an intriguing resemblance between dental plaque and non-host environments indicated by the TM7 evolution, suggesting that plaque may have served as a stepping stone for environmental microbes to adapt to host environments for some clades of microbes. Additionally, we report that prophages are widespread among oral-associated TM7, while absent from environmental TM7, suggesting that prophages may have played a role in adaptation of TM7 to the host environment. Conclusions Our data illuminate niche partitioning of enigmatic members of the oral cavity, including TM7, SR1, and GN02, and provide genomes for poorly characterized yet prevalent members of this biome, such as uncultivated Flavobacteriaceae.

60 APPLIED LIFE SCIENCES↗

DOE OSTI2024

Extrapolation of Type Ia Supernova Spectra into the Near-infrared Using Principal Component Analysis

Abstract We present a method of extrapolating the spectroscopic behavior of Type Ia supernovae (SNe Ia) in the near-infrared (NIR) wavelength regime up to 2.30 μ m using optical spectroscopy. Such a process is useful for accurately estimating K-corrections and other photometric quantities of SNe Ia in the NIR. A principal component analysis is performed on data consisting of Carnegie Supernova Project I & II optical and NIR FIRE spectra to produce models capable of making these extrapolations. This method differs from previous spectral template methods by not parameterizing models strictly by photometric light-curve properties of SNe Ia, allowing for more flexibility of the resulting extrapolated NIR flux. A difference of around −3.1% to −2.7% in the total integrated NIR flux between these extrapolations and the observations is seen here for most test cases including Branch core-normal and shallow-silicon subtypes. However, larger deviations from the observation are found for other tests, likely due to the limited high-velocity and broad-line SNe Ia in the training sample. Maximum-light principal components are shown to allow for spectroscopic predictions of the color-stretch light-curve parameter, s BV , within approximately ±0.1 units of the value measured with photometry. We also show these results compare well with NIR templates, although in most cases the templates are marginally more fitting to observations, illustrating a need for more concurrent optical+NIR spectroscopic observations to truly understand the diversity of SNe Ia in the NIR.

Astronomy & Astrophysics↗

NASA NTRS2002

Automation of Coordinated Planning Between Observatories: The Visual Observation Layout Tool (VOLT)

Fulfilling the promise of the era of great observatories, NASA now has more than three space-based astronomical telescopes operating in different wavebands. This situation provides astronomers with the unique opportunity of simultaneously observing a target in multiple wavebands with these observatories. Currently scheduling multiple observatories simultaneously, for coordinated observations, is highly inefficient. Coordinated observations require painstaking manual collaboration among the observatory staff at each observatory. Because they are time-consuming and expensive to schedule, observatories often limit the number of coordinated observations that can be conducted. In order to exploit new paradigms for observatory operation, the Advanced Architectures and Automation Branch of NASA's Goddard Space Flight Center has developed a tool called the Visual Observation Layout Tool (VOLT). The main objective of VOLT is to provide a visual tool to automate the planning of coordinated observations by multiple astronomical observatories. Four of NASA's space-based astronomical observatories - the Hubble Space Telescope (HST), Far Ultraviolet Spectroscopic Explorer (FUSE), Rossi X-ray Timing Explorer (RXTE) and Chandra - are enthusiastically pursuing the use of VOLT. This paper will focus on the purpose for developing VOLT, as well as the lessons learned during the infusion of VOLT into the planning and scheduling operations of these observatories.

Maks, Lori↗

NASA NTRS2005

Closed-Cycle Engine Program Used to Study Brayton Power Conversion

One form of power conversion under consideration in NASA Glenn Research Center's Thermal Energy Conversion Branch is the closed-Brayton-cycle engine. In the tens-of-kilowatts to multimegawatt class, the Brayton engine lends itself to potential space nuclear power applications such as electric propulsion or surface power. The Thermal Energy Conversion Branch has most recently concentrated its Brayton studies on electric propulsion for Prometheus. One piece of software used for evaluating such designs over a limited tradeoff space has been the Closed Cycle Engine Program (CCEP). The CCEP originated in the mid-1980s from a Fortran aircraft engine code known as the Navy/NASA Engine Program (NNEP). Components such as a solar collector, heat exchangers, ducting, a pumped-loop radiator, a nuclear heat source, and radial turbomachinery were added to NNEP, transforming it into a high-fidelity design and performance tool for closed-Brayton-cycle power conversion and heat rejection. CCEP was used in the 1990s in conjunction with the Solar Dynamic Ground Test Demonstration conducted at Glenn. Over the past year, updates were made to CCEP to adapt it for an electric propulsion application. The pumped-loop radiator coolant can now be n-heptane, water, or sodium-potassium (NaK); liquid-metal pump design tables were added to accommodate the NaK fluid. For the reactor and shield, a user can now elect to calculate a higher fidelity mass estimate. In addition, helium-xenon working-fluid properties were recalculated and updated.

Johnson, Paul K.↗

DOE OSTI2023

Multifunctional RF limiting amplifier

Multifunctional RF limiting amplifiers having various configurations and functions are disclosed. In a first configuration, the RF limiting amplifier includes an active load output circuit that allows one to adjust the output impedance based upon the anticipated connected load impedance. In a second configuration, the RF limiting amplifier includes a pair of emitter-followers to buffer the output of a first stage, allowing the RF limiting amplifier to drive one or more second stages. A third configuration includes a pair of RF limiting amplifiers with their outputs mixed to implement a down conversion function. The third configuration may be used to drive dual SAW resonators for detecting the presence of biological or chemical agents. The RF limiting amplifier may be implemented in either bipolar junction transistors or CMOS transistors.

Wessendorf, Kurt O.↗

DOE OSTI2023

Microbial production of high octane and high sensitivity olefinic ester biofuels

Abstract Background Advanced spark ignition engines require high performance fuels with improved resistance to autoignition. Biologically derived olefinic alcohols have arisen as promising blendstock candidates due to favorable octane numbers and synergistic blending characteristics. However, production and downstream separation of these alcohols are limited by their intrinsic toxicity and high aqueous solubility, respectively. Bioproduction of carboxylate esters of alcohols can improve partitioning and reduce toxicity, but in practice has been limited to saturated esters with characteristically low octane sensitivity. If olefinic esters retain the synergistic blending characteristics of their alcohol counterparts, they could improve the bioblendstock combustion performance while also retaining the production advantages of the ester moiety. Results Optimization of Escherichia coli isoprenoid pathways has led to high titers of isoprenol and prenol, which are not only excellent standalone biofuel and blend candidates, but also novel targets for esterification. Here, a selection of olefinic esters enhanced blendstock performance according to their degree of unsaturation and branching. E. coli strains harboring optimized mevalonate pathways, thioester pathways, and heterologous alcohol acyltransferases (ATF1, ATF2, and SAAT) were engineered for the bioproduction of four novel olefinic esters. Although prenyl and isoprenyl lactate titers were limited to 1.48 ± 0.41 mg/L and 5.57 ± 1.36 mg/L, strains engineered for prenyl and isoprenyl acetate attained titers of 176.3 ± 16.0 mg/L and 3.08 ± 0.27 g/L, respectively. Furthermore, prenyl acetate (20% bRON = 125.8) and isoprenyl acetate (20% bRON = 108.4) exhibited blend properties comparable to ethanol and significantly better than any saturated ester. By further scaling cultures to a 2-L bioreactor under fed-batch conditions, 15.0 ± 0.9 g/L isoprenyl acetate was achieved on minimal medium. Metabolic engineering of acetate pathway flux further improved titer to attain an unprecedented 28.0 ± 1.0 g/L isoprenyl acetate, accounting for 75.7% theoretical yield from glucose. Conclusion Our study demonstrated novel bioproduction of four isoprenoid oxygenates for fuel blending. Our optimized E. coli production strain generated an unprecedented titer of isoprenyl acetate and when paired with its favorable blend properties, may enable rapid scale-up of olefinic alcohol esters for use as a fuel blend additive or as a precursor for longer-chain biofuels and biochemicals.

09 BIOMASS FUELS↗

DOE OSTI2025

Benchmark microgravity experiments and computations for 3D dendritic-array stability in directional solidification

In this study, we present a comprehensive quantitative analysis of stability bands for dendritic arrays during directional solidification of a transparent succinonitrile-0.46 wt % camphor alloy, spanning a broad range of pulling velocities. Taking advantage of the microgravity environment aboard the International Space Station where most convection effects are suppressed, we obtain unique measurements that quantify the stable primary spacing range of spatially extended three-dimensional dendritic array structures under purely diffusive growth conditions. Through carefully designed velocity jump experiments and detailed examination of sub-grain boundary dynamics, we characterize key instabilities, including elimination and tertiary branching, shedding new light on the mechanisms governing dynamic dendritic spacing selection in extended 3D arrays. Phase field simulations are performed to characterize the stability limits of dendritic array structures for quantitative comparison with the flight experiments. Although the simulations capture general trends, significant deviations are noted at the upper stability boundary, indicating the influence of additional, unexplored factors. These findings contribute to a deeper understanding of dendritic growth dynamics and offer valuable benchmark data that could aid in refining predictive models and improving control of dendritic microstructures in metallurgical applications.

36 MATERIALS SCIENCE↗

DOE OSTI2024

Custodial symmetry breaking and Higgs boson signatures at the LHC

We discuss the simplest model that predicts a tree-level modification of the ρ parameter from a shift in the W -mass without changing the prediction for the Z mass. This model predicts a new neutral Higgs and two charged Higgses, with very similar masses and suppressed couplings to the Standard Model fermions. We discuss the decay properties of these new scalar bosons, and the main signatures at the Large Hadron Collider. Comparing these signatures for the first time to the latest measurements, we show that while masses around 200 GeV are excluded for some scenarios, over a wide range of model parameter space the new bosons can have a mass close to the electroweak scale without violating existing limits from experimental searches or destroying the agreement with measurements. We investigate the scenario where the new neutral Higgs is fermiophobic and has a large branching ratio into W gauge bosons and/or photons, and show that this could lead to a signal in the diphoton mass spectrum at low Higgs masses. We discuss the different signatures that can motivate new measurements and searches at the Large Hadron Collider. Published by the American Physical Society 2024

Astronomy & Astrophysics↗

DOE OSTI2024

Search for Inelastic Dark Matter in Events with Two Displaced Muons and Missing Transverse Momentum in Proton-Proton Collisions at s = 13 TeV

A search for dark matter in events with a displaced nonresonant muon pair and missing transverse momentum is presented. The analysis is performed using an integrated luminosity of 138 fb − 1 of proton-proton ( p p ) collision data at a center-of-mass energy of 13 TeV produced by the LHC in 2016–2018. No significant excess over the predicted backgrounds is observed. Upper limits are set on the product of the inelastic dark matter production cross section σ ( p p → A ′ → χ 1 χ 2 ) and the decay branching fraction B ( χ 2 → χ 1 μ + μ − ) , where A ′ is a dark photon and χ 1 and χ 2 are states in the dark sector with near mass degeneracy. This is the first dedicated collider search for inelastic dark matter. © 2024 CERN, for the CMS Collaboration 2024 CERN

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

NASA NTRS1998

Search for Interstellar Water in the Translucent Molecular Cloud Toward HD 154368

We report an upper limit of 9 x 10(exp 12)/sq cm on the column density of water in the translucent cloud along the line of sight toward HD 154368. This result is based on a search for the C-X band of water near 1240 A carried out using the Goddard High-Resolution Spectrograph (GHRS) of the Hubble Space Telescope. Our observational limit on the water abundance, together with detailed chemical models of translucent clouds and previous measurements of OH along the line of sight, constrains the branching ratio in the dissociative recombination of H3O(+) to form water. We find at the 3 sigma level that no more than 30% of dissociative recombinations of H3O(+) can lead to H2O. The observed spectrum also yielded high-resolution observations of the Mg II doublet at 1239.9 A and 1240.4 A, allowing the velocity structure of the dominant ionization state of magnesium to be studied along the line of sight. The Mg II spectrum is consistent with GHRS observations at lower spectral resolution that were obtained previously but allow an additional velocity component to be identified.

Spaans, Marco↗

NASA NTRS2002

Simultaneous Spectral and Timing Observations of Accreting Neuron Stars

The goal of this proposal is to perform simultaneous x-ray spectral and millisecond timing observations of accreting neutron stars to further our understanding of their accretion dynamics and in the hope of using these systems as probes of the physics of strong gravitational fields. NAG5-9104 is the successor grant to NAG5-8408. Observations using the Rossi X-Ray Timing Explorer (RXTE) and BeppoSAX were performed of 4U1702-429, 4U1735-44, and Cyg X-2. Unfortunately, only a small fraction of the approved observing time was obtained for the first two targets and the data are of limited scientific value. Data analysis has been completed on the observations of Cyg X-2. We discovered a correlation between the frequency of the horizontal branch oscillations (HBO) and a soft, thermal component of the x-ray spectrum likely associated with emission from the accretion disk. This correlation may place constraints on models of the oscillations. A paper based on these results appeared in the Astrophysical Journal.

Kaaret, P.↗

DOE OSTI2020

Impact Response of Cold Spray Deposited Materials

Solid-state cold spraying (CS) of metals and respective blends is becoming increasingly attractive compared to conventional high temperature processes due to the unique properties such as increased yield strength, low ductility, and differences in tensile and compressive strengths that result from microstructural features due to the CS process. Here we report the results of plate impact experiments applied to CS deposits of tantalum (Ta), niobium (Nb), and a tantalum- niobium blend (TaNb). These methods allowed for definition of the Hugoniot for each material type and allowed for assessment of the Hugoniot Elastic Limit (HEL). Scanning electron microscopy was used on recovered samples to characterize the fracture mechanism during spallation.

36 MATERIALS SCIENCE↗

DOE OSTI2022

Frhodo: A program for simulating chemical kinetic measurements and optimizing kinetic mechanisms

Chemical kinetic simulations are frequently used to extract kinetic and mechanistic information from experimental data by fitting simulations to the data. For simple systems of consecutive single channel reactions this is a relatively straightforward task. However, as the complexity of the system increases and particularly for systems involving competitive multi-channel reactions manually optimizing a mechanism to obtain the best fits to a range of experimental data quickly becomes a time-intensive and challenging process, especially when rate coefficients have pressure and temperature dependencies. There is considerable potential for exploiting automated optimization methods to rapidly screen reaction mechanisms and optimize the fit by adjusting rate coefficients within well-defined constraints. A new chemical kinetics simulation program, Frhodo, has been developed for this purpose. The program allows either manual simulation of individual experiments or fully automated optimization against a range of experimental data by adjusting user selected reactions within user defined constraints. Frhodo incorporates machine learning-based optimization routines that work by either minimizing standardized residuals or through Bayesian parameter estimation. Frhodo's optimization capabilities are demonstrated by reexamining two previously studied systems of relevance to combustion. Dissociation of diacetyl followed by recombination of methyl radicals is an example of a sequence of single channel, consecutive reactions. Pyrolysis of 2-methyl furan exemplifies multi-channel, unimolecular reactions that are often encountered and have temperature and pressure dependencies in the rate coefficients of the channels and branching ratios between them. For both systems the optimization routines resulted in solutions similar to the original studies. Finally, comments are made on the strengths and limitations of the approach.

0D simulation↗