Advanced manned launch system study status
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N-ethylmaleimide (NEM) inhibits the ATPase of H. saccharovorum in a nucleotide protectable manner. The bulk of 14C-NEM is incorporated into subunit 1. Inhibition kinetics indicated a single binding site. To determine the sequence around this site, cyanogen bromide peptides of NEM-labeled ATPase enzyme were prepared and separated on Tris-Tricine gels. Autoradiography indicated that the NEM binding site is probably located in a fragment of Mr 10-12 K. This result will be confirmed by N-terminal sequencing of the peptide. Since the cysteinyl residue, to which NEM is bound, may be located at the C-terminal end, purification and proteolytic treatment of the 10 K peptide will be required. One inhibitor of V-type ATPases, fluoresceinisothiocyanate (FITC) inhibited also the ATPase of H. saccharovorum. Preliminary results indicated protection against inhibition by nucleotides. Localization of the binding sited to the major subunits is in progress. An extraction procedure for the membrane sector of the ATPase complex of H. saccharovorum yielded a preparation which was enriched in a peptide of Mr 5 500. Experiments to test the immunological crossreaction with subunit c from the Escherichia coli F-type ATPase and the labeling with 14C-DCCD are currently carried out. Polyclonal antiserum to the smaller of the major subunits of the ATPase from H. saccharovorum (subunit ll) reacts in Western blots strongly with the alpha and beta subunits of the F1 ATPase of E. coli, suggesting highly conserved regions on both types of ATPases. To elucidate further the regions of homology, cyanogen bromide peptides of the beta subunits were prepared for sequence analysis.
The Alternate Propulsion Subsystem Concepts contract had five tasks defined for the first year. The tasks were: F-1A Restart Study, J-2S Restart Study, Propulsion Database Development, Space Shuttle Main Engine (SSME) Upper Stage Use, and CER's for Liquid Propellant Rocket Engines. The detailed study results, with the data to support the conclusions from various analyses, are being reported as a series of five separate Final Task Reports. Consequently, this volume only reports the required programmatic information concerning Computer Aided Design Documentation, and New Technology Reports. A detailed Executive Summary, covering all the tasks, is also available as Volume 1.
N-ethylmaleimide (NEM) inhibits the ATPase of H. saccharovorum in a nucleotide protectable manner. The bulk of C-14 NEM is incorporated into subunit one. Cyanogen bromide cleavage of labeled subunit one indicated that NEM bound to a peptide of a Mr of about 8,900. Thus, Cys 262 (H. salinarium numbering) may be the NEM binding site. Cyanogen bromide fragments have been submitted for sequencing. To prove the presence of three Cys residues in subunit one, alkaline cleavage following treatment with NTCB was carried out. Thiol reagents such as p-chloro mercuri phenyl sulfonate also inhibited the ATPase. However, this inhibition was not nucleotide-protectable, suggesting a different location and role for the PCMS-sensitive Cys. The proteolipid which was extracted with chloroform/methanol from the membranes of H. saccharovorum cross-reacted with an antiserum against subunit c (the DCCD-binding protein) of Escherichia coli. Following labeling of membranes from H. saccharovorum with C-14 DCCD under conditions, which inhibited ATP synthesis, the isotope was incorporated into one protein of Mr of about 6,500. Thus, the proteolipid of H. saccharovorum and the DCCD-labeled peptide may be identical. If so, these results suggest that the proteolipid is a component of the membrane sector of an archaeal F-type ATP synthase.
During the past decade, a growing need to conduct regional assessments of long-term trends of ecosystem behavior and the technology to meet this need have converged. The Century model is the product of research efforts initially intended to develop a general model of plant-soil ecosystem dynamics for the North American central grasslands. This model is now being used to simulate plant production, nutrient cycling, and soil organic matter dynamics for grassland, crop, forest, and shrub ecosystems in various regions of the world, including temperate and tropical ecosystems. This paper will focus on the philosophical approach used to develop the structure of Century. The steps included were model simplification, parameterization, and testing. In addition, the importance of acquiring regional data bases for model testing and the present regional application of Century in the Great Plains, which focus on regional ecosystem dynamics and the effect of altering environmental conditions, are discussed.
The purpose of the TA-2 contract was to provide advanced launch vehicle concept definition and analysis to assist NASA in the identification of future launch vehicle requirements. Contracted analysis activities included vehicle sizing and performance analysis, subsystem concept definition, propulsion subsystem definition (foreign and domestic), ground operations and facilities analysis, and life cycle cost estimation. This document is part of the final report for the TA-2 contract. The final report consists of three volumes: Volume 1 is the Executive Summary, Volume 2 is Technical Results, and Volume 3 is Program Cost Estimates. The document-at-hand, Volume 1, provides a summary description of the technical activities that were performed over the entire contract duration, covering three distinct launch vehicle definition activities: heavy-lift (300,000 pounds injected mass to low Earth orbit) launch vehicles for the First Lunar Outpost (FLO), medium-lift (50,000-80,000 pounds injected mass to low Earth orbit) launch vehicles, and single-stage-to-orbit (SSTO) launch vehicles (25,000 pounds injected mass to a Space Station orbit).
The purpose of the TA-2 contract was to provide advanced launch vehicle concept definition and analysis to assist NASA in the identification of future launch vehicle requirements. Contracted analysis activities included vehicle sizing and performance analysis, subsystem concept definition, propulsion subsystem definition (foreign and domestic), ground operations and facilities analysis, and life cycle cost estimation. The basic period of performance of the TA-2 contract was from May 1992 through May 1993. No-cost extensions were exercised on the contract from June 1993 through July 1995. This document is part of the final report for the TA-2 contract. The final report consists of three volumes: Volume 1 is the Executive Summary, Volume 2 is Technical Results, and Volume 3 is Program Cost Estimates. The document-at-hand, Volume 3, provides a work breakdown structure dictionary, user's guide for the parametric life cycle cost estimation tool, and final report developed by ECON, Inc., under subcontract to Lockheed Martin on TA-2 for the analysis of heavy lift launch vehicle concepts.
The dicyclohexyl carbodiimide (DCCD)- binding site of the membrane ATPase from Halobacterium saccharovorum was investigated during earlier periods of this Cooperative Agreement and was localized to a cyanogen bromide fragment of subunit 2 from amino acids 379 (Glu) to 442 (Met). Although the exact position of the reactive amino acid (probably a glutamic acid) has not yet been determined, the data, together with recently obtained immuno reactions and sequences of Cyanogen Bromide (CNBr) fragments from E.coli F-ATPase, suggested subunit interactions in the halobacterial ATPase which had not been recognized before. They also provided evidence for the presence of a gamma subunit in the halobacterial ATPase, and for a stretch of a amino acids similar to the 'catch' between beta and gamma in bovine F-ATPase. The evolutionary implications of these findings are twofold: first, halobacterial (or archaebacterial) ATPases appear as complex as those from higher organisms - no simpler versions of these membrane enzymes are known to date; second, a monophyletic origin of the energy-transducing ATPases is becoming more apparent, and - together with other data - the split into V- and F-ATPases may have occurred much later than had been previously thought (i.e., after the split into Archaea and Bacteria). Other work included the characterization of an extremely halophilic isolate (Halococcus salifodinae ) from Permian salt sediments. This organism appeared to be an autotrophic halobacterium; its incorporation of C02 was investigated.
The Alternate Propulsion Subsystem Concepts contract had seven tasks defined for this report. The tasks were: F-1A Restart Study, J-2S Restart Study, Propulsion Database Development, SSME Upper Stage Use, CERs for Liquid Propellant Rocket Engines, Advanced Low Cost Engines, and Tripropellant Comparison Study. The detailed study results, with the data to support the conclusions from various analyses, are being reported as a series of five separate Final Task Reports. Consequently, this volume only reports the required programmatic information concerning Computer Aided Design Documentation, and New Technology Reports. A detailed Executive Summary, covering all the tasks, is also available as Volume I of this report.
Propulsion for acceptable supersonic passenger transport aircraft is primarily impacted by the very high jet noise characteristics of otherwise attractive engines. The mixed flow turbofan, when equipped with a special ejector nozzle seems to be the best candidate engine for this task of combining low jet noise with acceptable flight performance. Design, performance, and operation aspects of mixed flow turbofans are discussed. If the special silencing nozzle is too large, too heavy, or not as effective as expected, alternative concepts in mixed flow engines should be examined. Presented herein is a brief summary of efforts performed under cooperative agreement NCC3-193. Three alternative engine concepts, conceived during this study effort, are herein presented and their limitations and potentials are described. These three concepts intentionally avoid the use of special silencing nozzles and achieve low jet noise by airflow augmentation of the engine cycle.
This study defines a family of advanced technology Stratified Charge Rotary Engines (SCRE) appropriate for the enablement of the development of a new generation of general aviation aircraft. High commonality, affordability, and environmental compatibility are considerations influencing the family composition and ratings. The SCRE family is comprised of three engines in the 70 Series (40 cu in. displacement per rotor), i.e. one, two, and four rotor and two engines in the 170 Series (105 cu in. displacement per rotor), i.e., two and four rotor. The two rotor engines are considered the primary engines in each series. A wide power range is considered covering 125 to 2500 HP through growth and compounding/dual pac considerations. Mission requirements, TBO, FAA Certification, engine development cycles, and costs are examined. Comparisons to current and projected reciprocating and turbine engine configurations in the 125 to 1000 HP class are provided. Market impact, estimated sales, and U.S. job creation (R&D, manufacturing and infractures) are examined.
In this study, new technology engines were defined in two power classes: a 200 hp class, for a light, 4-place personal aircraft, and a 1500 pound thrust class for a twin-engined, 6 place business jet type aircraft. The engines were evaluated for retrofitting suitable current production aircraft for comparison to the existing engines. The engines were evaluated for performance using a typical mission for each aircraft, as well as a variant mission to further appraise performance.Issues of cost, safety, maintenance, and reliability were also addressed. Manufacturing plans were then constructed.
A CFD analysis was performed on a Blended Wing Body (BWB) aircraft with advanced, turbofan engines analyzing various inlet configurations atop the aft end of the aircraft. The results are presented showing that the optimal design for best aircraft fuel efficiency would be a configuration with a partially buried engine, short offset diffuser using active flow control, and a D-shaped inlet duct that partially ingests the boundary layer air in flight. The CFD models showed that if active flow control technology can be satisfactorily developed, it might be able to control the inlet flow distortion to the engine fan face and reduce the powerplant performance losses to an acceptable level. The weight and surface area drag benefits of a partially submerged engine shows that it might offset the penalties of ingesting the low energy boundary layer air. The combined airplane performance of such a design might deliver approximately 5.5% better aircraft fuel efficiency over a conventionally designed, pod-mounted engine.
The volatilization of silica (SiO2) to silicon hydroxides and oxyhydroxides because of reaction with water vapor is important in a variety of high-temperature corrosion processes. For example, the lifetimes of silicon carbide (SiC) and silicon nitride (Si3N4) - based components in combustion environments are limited by silica volatility. To understand and model this process, it is essential to have accurate thermodynamic data for the formation of volatile silicon hydroxides and oxyhydroxides.
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