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At least 469 records · Page 26

Multiple Probe Measurements at Uranus Motivated By Spatial Variability

Motivation: Spatial variations in the temperature field and composition of Uranus' atmosphere demonstrate a need for multiple entry probes to characterize vertical profiles in multiple locations. We will review variation of composition and temperature, which are produced by dynamical processes on a range of scales from global (polar anomalies, zonal bands) to regional (vortices, storms) [1–2]. In particular, the spatial variation of convective activity is not well understood based on existing remote sensing observations [3]. Understanding how these processes operate, and how they modulate variable composition, is key to constraining bulk atmospheric abundances. Abundances in turn provide cosmochemical constraints on planetary origins. Secondary probes at Uranus: Key measurements for secondary probes are temperature-pressure profiles, along with compositional profiles. Radio occultations are limited to shallow levels less than ~2 bar [4]. In situ temperature-pressure measurements (or atmospheric structure measurements) can unambiguously extend these results to deeper levels. Measurements with vertical resolution of at least 2 km are needed to characterize anomalies like the 1.2-bar feature from the Voyager 2 occultation at 2–6° S [4], which supports a range of temperature gradients depending on assumptions of composition. Simultaneous measurements of temperature, pressure, and composition are key to understanding this class of features. Species such as methane, hydrogen sulfide, and ammonia must be measured along a probe descent profile because they vary over several orders of magnitude due to the strong temperature dependence of their saturation vapor pressures [e.g., 5]. Results of these measurements can be interpreted to understand the potential for moist convective activity in the atmosphere. Lessons from the other giant planets: On Jupiter, the Galileo Probe's entry into a meteorologically distinct five-micron hot spot led many to interpret the local composition as column-stretched, so that well-mixed abundances were reached at deeper levels than in surrounding, unperturbed regions [6–8]. Ground-based microwave measurements and Juno data now indicate that the deep depletion of ammonia is a very widespread atmospheric characteristic not limited to 5-µm hot spots [9–11]. But in the absence of multiple probes, we do not know if the other volatiles H2S and H2O behave in the same way. The open questions for the Jupiter case strongly motivate sending multiple probes to Uranus. On Saturn, retrievals of NH3 and PH3 abundances at shallow levels vary with latitude [12], but it is unknown how deep these differences extend, which is why a Saturn probe (or probes) was a mission theme for NASA's New Frontiers 4 and 5 opportunities [13]. Challenges for secondary probes: Cost is an issue due to the perception that it involves sacrifices to other mission elements. Spacecraft trajectories may be constrained by needs for orbit insertion that limit probe deliveries to different latitudes, and additional limitations may be placed on communication windows for probe descent phases (particularly if multiple probes are released from the orbiter simultaneously) [14]. Finally, composition sensors for miniature secondary probes are not at the required technological maturity [15]. Mass spectrometers are typically too large, massive, and powerhungry, while smaller nanosensors are only beginning to be developed for planetary missions. Finally, the need for probe survival heating is most easily met by radioisotope heat sources, but these require regulatory approvals that are even more difficult to satisfy compared to standard environmental reviews [16], unless the secondary probe is designed as a core element of a mission. References: [1] Molter E.M., et al. (2021) PSJ, 2, 3. [2] Rowe-Gurney N., et al. (2021) Icar, 365, 114506. [3] Hueso R., Sánchez-Lavega A. (2019) SSRv, 215, 52. [4] Lindal G.F., et al. (1987) JGR, 92, 14987– 15001. [5] Simon A.A., et al. (2022) RemS, 14, 1518. [6] Atreya, S.K., et al. (1997) in The Three Galileos: The Man, the Spacecraft, the Telescope, pp. 249–260 (C. Barbieri et al., eds.). [7] Showman A.P., Ingersoll A.P. (1998) Icar, 132, 205–220. [8] Friedson A.J. (2005) Icar, 177, 1–17. [9] de Pater I., et al. (2001) Icar, 149, 66–78. [10] Li C., et al. (2017) GeoRL, 44, 5317–5325. [11] de Pater I., et al. (2019) Icar, 322, 168–191. [12] Fletcher L.N., et al. (2009) Icar, 202, 543–564. [13] National Research Council (2011) Vision and Voyages. [14] Sayanagi K.M., et al. (2020) SSRv, 216, 72. [15] Wong M.H. et al. (2021) BAAS, 53, 486. [16] Zide A., Mendoza-Hill A., Cheney D. (2022) COSPAR Abstracts H0.6-0012-22.

Michael H Wong↗

Matrix multiplication operations with data pre-conditioning in a high performance computing architecture

Mechanisms for performing matrix multiplication operations with data pre-conditioning in a high performance computing architecture are provided. A vector load operation is performed to load a first vector operand of the matrix multiplication operation to a first target vector register. A load and splat operation is performed to load an element of a second vector operand and replicating the element to each of a plurality of elements of a second target vector register. A multiply add operation is performed on elements of the first target vector register and elements of the second target vector register to generate a partial product of the matrix multiplication operation. The partial product of the matrix multiplication operation is accumulated with other partial products of the matrix multiplication operation.

97 MATHEMATICS AND COMPUTING↗

Multiplicity dependent 𝐽/𝜓 and 𝜓⁡(2⁢𝑆) production at forward and backward rapidity in 𝑝 + 𝑝 collisions at $\sqrt{𝑠}$ = 200 GeV

Recent measurements of 𝐽/𝜓 production as a function of event charged-particle multiplicity at the collision energies of both the Large Hadron Collider (LHC) and the Relativistic Heavy Ion Collider (RHIC) show enhanced 𝐽/𝜓 production yields with increasing multiplicity. One potential explanation for this type of dependence is multiparton interactions (MPI). We present the first study of potential autocorrelations at RHIC energies and forward and backward rapidity of self-normalized 𝐽/𝜓 yields and 𝜓⁡(2⁢𝑆) to 𝐽/𝜓 ratio, as a function of self-normalized multiplicity in 𝑝 + 𝑝 collisions. In addition, detailed pythia studies tuned to RHIC energies were performed to investigate the MPI impacts. We find that the PHENIX data at RHIC are consistent with recent LHC measurements and can only be described by pythia calculations that include MPI effects. The forward and backward 𝜓⁡(2⁢𝑆) to 𝐽/𝜓 ratio is found to be less dependent on the charged-particle multiplicity.

73 NUCLEAR PHYSICS AND RADIATION PHYSICS↗

ALTERNATIVES TO HELIUM-3 FOR NEUTRON MULTIPLICITY DETECTORS

Collaboration between the Pacific Northwest National Laboratory (PNNL) and the Los Alamos National Laboratory (LANL) is underway to evaluate neutron detection technologies that might replace the high-pressure helium (3He) tubes currently used in neutron multiplicity counter for safeguards applications. The current stockpile of 3He is diminishing and alternatives are needed for a variety of neutron detection applications including multiplicity counters. The first phase of this investigation uses a series of Monte Carlo calculations to simulate the performance of an existing neutron multiplicity counter configuration by replacing the 3He tubes in a model for that counter with candidate alternative technologies. These alternative technologies are initially placed in approximately the same configuration as the 3He tubes to establish a reference level of performance against the 3He-based system. After these reference-level results are established, the configurations of the alternative models will be further modified for performance optimization. The 3He model for these simulations is the one used by LANL to develop and benchmark the Epithermal Neutron Multiplicity Counter (ENMC) detector, as documented by H.O. Menlove, et al. in the 2004 LANL report LA-14088. The alternative technologies being evaluated are the boron-tri-fluoride-filled proportional tubes, boron-lined tubes, and lithium coated materials previously tested as possible replacements in portal monitor screening applications, as documented by R.T. Kouzes, et al. in the 2010 PNNL report PNNL-72544 and NIM A 623 (2010) 1035–1045. The models and methods used for these comparative calculations will be described and preliminary results shown

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗

Ultrafast Supercontinuum Spectroscopy of Carrier Multiplication and Biexcitonic Effects in Excited States of PbS Quantum Dots

We examine the population dynamics of multiple excitons in PbS quantum dots using spectrally resolved ultrafast supercontinuum transient absorption (SC-TA) measurements. We simultaneously probe the first three excitonic transitions. The transient spectra show the presence of bleaching of absorption for the 1S{sub h}-1S{sub e} transition, as well as transients associated with the 1P{sub h}-1P{sub e} transition. We examine signatures of carrier multiplication (multiple excitons arising from a single absorbed photon) from analysis of the bleaching features in the limit of low absorbed photon numbers (<N{sub abs} {approx} 10{sup -2}) for pump photon energies from two to four times that of the band gap. The efficiency of multiple-exciton generation is discussed both in terms of the ratio between early- to long-time transient absorption signals and of a broadband global fit to the data. Analysis of the population dynamics shows that bleaching associated with biexciton population is red shifted with respect to the single exciton feature, which is in accordance with a positive binding energy for the biexciton.

14 SOLAR ENERGY↗

The MARVEL assembly for neutron multiplication

A new multiplying test assembly is under development at Idaho National Laboratory to support research, validation, evaluation, and learning. The item is comprised of three stacked, highly-enriched uranium (HEU) cylinders, each 11.4 cm in diameter and having a combined height of up to 11.7 cm. The combined mass of all three cylinders is 20.3 kg of HEU. Calculations for the bare configuration of the assembly indicate a multiplication level of >3.5 (keff=0.72). Reflected configurations of the assembly, using either polyethylene or tungsten, are possible and have the capability of raising the assembly's multiplication level to greater than 10. This paper describes simulations performed to assess the assembly's multiplication level under different conditions and describes the resources available at INL to support the use of these materials. We also describe some preliminary calculations and test activities using the assembly to study neutron multiplication.

99 GENERAL AND MISCELLANEOUS↗

Nonlinear dynamic analysis of solution multiplicity of buoyancy ventilation in a typical underground structure

Buoyancy ventilation is widely used in underground buildings, such as underground hydropower stations. Multiple solutions of buoyancy ventilation may exist in those underground structures. In this study, we developed a transient model comprising an ordinary differential equation system to describe buoyancy ventilation patterns in typical two-zone underground structures. Additionally, the accuracy of the model was validated. Nonlinear dynamical analysis was conducted to study multiple steady-state airflow. According to mathematical derivation, the configuration of one local heat source at the bottom corner introduces two stable solutions. The criterion to determine the stability and existence of solutions for more general scenarios was developed. Using this criterion, we obtained the multiple steady states of any two-zone underground buildings for different stack height ratios and the strength ratios of the heat sources. Furthermore, this criterion can be adopted for the design of buoyancy ventilation or natural smoke ventilation systems. Designers can change the height ratio of the stack or the heat ratio of two zones to induce the desired ventilation patterns. Finally, a case study was conducted with field measurements to demonstrate the use of the nonlinear dynamical analysis method to investigate the multiple steady states of buoyancy ventilation. Through the case study, we validated that the proposed criterion could produce the same result as the nonlinear dynamical analysis.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

At-power subcritical multiplication in the Advanced Test Reactor during nuclear requalification testing

Power division information during nuclear requalification of the Advanced Test Reactor (ATR) is of considerable interest as an importance function for observed changes to core reactivity. The degree to which a given physical subdivision of a critical reactor acts as a neutron source for other lobes is not analytically characterized for general application. When ATR operates at power, individual power-producing lobes rely on each other as neutron sources in order to maintain constant power, which in general requires either exactly critical multiplication within a reactor or an external neutron source. Here, this work shows that fuel element and lobe powers in ATR can be related with subcritical multiplication theory. Subcritical multiplication factors are computed with a physically validated analytical method based on actual at-power operation, quantifying for each lobe its dependence on other lobes as an external neutron source. This explanation is significant for ATR due to the desire to irradiate a large variety of experiments simultaneously, each having its impact on the core neutron population. For any physical subdivision of any other critical reactor, it is likewise true that the subdivision undergoes only subcritical multiplication.

21 - SPECIFIC NUCLEAR REACTORS AND ASSOCIATED PLAN↗

Multiple scattering of surface waves by cavities in a half-space

Scattering of surface waves from multiple two-dimensional cavities at the surface of a homogenous, isotropic, linearly elastic half-space is analyzed in this work. For the case of multiple cavities, the scattered field is shown to be equivalent to the total radiation from the distributions of tractions, calculated from the incident wave, over the surfaces of the cavities. The multiple-scattering model is obtained from known single-scattering calculation for a cavity by the use of the self-consistent method. The second order approximation to the multiple-scattering problem by a random distribution of cavities is then considered and solved analytically. The vertical displacement at some distance from the cavities is calculated and verified by the solution of the same problem obtained by the boundary element method (BEM). The analytical and BEM results are graphically displayed and show good agreement when the depths of the cavities are small compared to the wavelength.

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗

Neutron Multiplicity Counting Moments for Fissile Mass Estimation in Scatter-Based Neutron Detection Systems

Neutron multiplicity counting (NMC) techniques are widely used for nuclear materials accountability and international safeguards applications to quantitatively evaluate characteristic properties pertaining to fissile material. Mathematical models for NMC moments have been previously derived for systems that use capture-based detectors; however, these models are not applicable when scatter-based detectors are used because of “neutron cross talk.” Neutron cross talk occurs when a single neutron scatters and deposits energy above threshold into multiple detectors causing spurious increase in multiplicity counts; this, in turn, has caused fissile mass to be overestimated when not treated. In this paper, we propose new mathematical models derived from point kinetics to correct for neutron cross-talk effects up to any arbitrary order N, where N denotes the maximum number of counts a single neutron can cause. The new models were used to estimate the fissile mass of plutonium metal and oxide samples with effective 240 Pu mass ranging from 2.5 to 250 g. The adequacy of the models was confirmed using simulations of a conceptual scatter-based neutron multiplicity counter (e.g., organic scintillators) using MCNPX v2.7e with the PoliMi fission event generating extension. The fissile mass estimates with no correction for neutron cross-talk events yielded an average relative deviation from the true 240 Pu eff mass of 55.94% and 84.56% for metal and oxide samples, respectively. When neutron cross-talk events of order N = 2 are included in the model, the fissile mass estimates yielded an average relative deviation of 11.89% for metal and 13.21% for oxide samples. Accounting for neutron cross-talk events of order N = 3 resulted in fissile mass estimates with an average relative deviation of 9.58% and 10.51% for metal and oxide samples, respectively. These mass estimates were compared to a reference case (i.e., no neutron crosstalk effects) that yielded an average relative deviation of 6.81% and 4.77% for metal and oxide samples, respectively. In conclusion, the discrepancy between the estimates from the proposed model and the reference case is attributed to the assumed value of N, which sets a finite upper bound on the order of cross-talk events the model treats (i.e., the model for N = 3 assumes that a neutron will never cause more than three counts).

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

THE ASTRALUX LARGE M-DWARF MULTIPLICITY SURVEY

We present the results of an extensive high-resolution imaging survey of M-dwarf multiplicity using the Lucky Imaging technique. The survey made use of the AstraLux Norte camera at the Calar Alto 2.2 m telescope and the AstraLux Sur camera at the ESO New Technology Telescope in order to cover nearly the full sky. In total, 761 stars were observed (701 M-type and 60 late K-type), among which 182 new and 37 previously known companions were detected in 205 systems. Most of the targets have been observed during two or more epochs, and could be confirmed as physical companions through common proper motion, often with orbital motion being confirmed in addition. After accounting for various bias effects, we find a total M-dwarf multiplicity fraction of 27% {+-} 3% within the AstraLux detection range of 0.''08-6'' (semimajor axes of {approx}3-227 AU at a median distance of 30 pc). We examine various statistical multiplicity properties within the sample, such as the trend of multiplicity fraction with stellar mass and the semimajor axis distribution. The results indicate that M-dwarfs are largely consistent with constituting an intermediate step in a continuous distribution from higher-mass stars down to brown dwarfs. Along with other observational results in the literature, this provides further indications that stars and brown dwarfs may share a common formation mechanism, rather than being distinct populations.

79 ASTRONOMY AND ASTROPHYSICS↗

AN ALMA SURVEY OF SUBMILLIMETER GALAXIES IN THE EXTENDED CHANDRA DEEP FIELD SOUTH: SOURCE CATALOG AND MULTIPLICITY

We present an Atacama Large Millimeter/submillimeter Array (ALMA) Cycle 0 survey of 126 submillimeter sources from the LABOCA ECDFS Submillimeter Survey (LESS). Our 870 {mu}m survey with ALMA (ALESS) has produced maps {approx}3 Multiplication-Sign deeper and with a beam area {approx}200 Multiplication-Sign smaller than the original LESS observations, doubling the current number of interferometrically-observed submillimeter sources. The high resolution of these maps allows us to resolve sources that were previously blended and accurately identify the origin of the submillimeter emission. We discuss the creation of the ALESS submillimeter galaxy (SMG) catalog, including the main sample of 99 SMGs and a supplementary sample of 32 SMGs. We find that at least 35% (possibly up to 50%) of the detected LABOCA sources have been resolved into multiple SMGs, and that the average number of SMGs per LESS source increases with LESS flux density. Using the (now precisely known) SMG positions, we empirically test the theoretical expectation for the uncertainty in the single-dish source positions. We also compare our catalog to the previously predicted radio/mid-infrared counterparts, finding that 45% of the ALESS SMGs were missed by this method. Our {approx}1.''6 resolution allows us to measure a size of {approx}9 kpc Multiplication-Sign 5 kpc for the rest-frame {approx}300 {mu}m emission region in one resolved SMG, implying a star formation rate surface density of 80 M{sub Sun} yr{sup -1} kpc{sup -2}, and we constrain the emission regions in the remaining SMGs to be <10 kpc. As the first statistically reliable survey of SMGs, this will provide the basis for an unbiased multiwavelength study of SMG properties.

79 ASTRONOMY AND ASTROPHYSICS↗

Angle-resolved photon-coincidence measurements in a multiple-scattering medium

We present angle-resolved correlation measurements between photons after propagation through a three-dimensional disordered medium. The multiple-scattering process induces photon correlations that are directly measured for light sources with different photon statistics. We find that multiple-scattered photons between different angular directions with angles much larger than the average speckle width are strongly correlated. The time dependence of the angular photon correlation function is investigated, and the coherence time of the light source is determined. Our results are found to be in excellent agreement with the continuous mode quantum theory of multiple scattering of light. The presented experimental technique is essential in order to study quantum phenomena in multiple-scattering random media, such as quantum interference and quantum entanglement of photons.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

Measurement of the Multiple-Muon Charge Ratio in the MINOS Far Detector

The charge ratio, Rμ=Nμ+/Nμ-, for cosmogenic multiple-muon events observed at an underground depth of 2070 mwe has been measured using the magnetized MINOS Far Detector. The multiple-muon events, recorded nearly continuously from August 2003 until April 2012, comprise two independent data sets imaged with opposite magnetic field polarities, the comparison of which allows the systematic uncertainties of the measurement to be minimized. The multiple-muon charge ratio is determined to be Rμ=1.104±0.006(stat)-0.010+0.009(syst). This measurement complements previous determinations of single-muon and multiple-muon charge ratios at underground sites and serves to constrain models of cosmic-ray interactions at TeV energies.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

Multiplicative Shot-Noise: A New Route to Stability of Plastic Networks

Fluctuations of synaptic weights, among many other physical, biological, and ecological quantities, are driven by coincident events of two “parent” processes. Here we propose a multiplicative shot-noise model that can capture the behaviors of a broad range of such natural phenomena, and analytically derive an approximation that accurately predicts its statistics. We apply our results to study the effects of a multiplicative synaptic plasticity rule that was recently extracted from measurements in physiological conditions. Using mean-field theory analysis and network simulations, we investigate how this rule shapes the connectivity and dynamics of recurrent spiking neural networks. The multiplicative plasticity rule is shown to support efficient learning of input stimuli, and it gives a stable, unimodal synaptic-weight distribution with a large fraction of strong synapses. The strong synapses remain stable over long times but do not “run away.” Our results suggest that the multiplicative shot-noise offers a new route to understand the tradeoff between flexibility and stability in neural circuits and other dynamic networks.

59 BASIC BIOLOGICAL SCIENCES↗

MPACT Fast Neutron Multiplicity System Design Concepts

This report documents work performed by Idaho National Laboratory and the University of Michigan in fiscal year (FY) 2012 to examine design parameters related to the use of fast-neutron multiplicity counting for assaying plutonium for materials protection, accountancy, and control purposes. This project seeks to develop a new type of neutron-measurement-based plutonium assay instrument suited for assaying advanced fuel cycle materials. Some current-concept advanced fuels contain high concentrations of plutonium; some of these concept fuels also contain other fissionable actinides besides plutonium. Because of these attributes the neutron emission rates of these new fuels may be much higher, and more difficult to interpret, than measurements made of plutonium-only materials. Fast neutron multiplicity analysis is one approach for assaying these advanced nuclear fuels. Studies have been performed to assess the conceptual performance capabilities of a fast-neutron multiplicity counter for assaying plutonium. Comparisons have been made to evaluate the potential improvements and benefits of fast-neutron multiplicity analyses versus traditional thermal-neutron counting systems. Fast-neutron instrumentation, using for example an array of liquid scintillators such as EJ-309, have the potential to either a) significantly reduce assay measurement times versus traditional approaches, for comparable measurement precision values, b) significantly improve assay precision values, for measurement durations comparable to current-generation technology, or c) moderating improve both measurement precision and measurement durations versus current-generation technology. Using the MCNPX-PoliMi Monte Carlo simulation code, studies have been performed to assess the doubles-detection efficiency for a variety of counter layouts of cylindrical liquid scintillator detector cells over one, two, and three rows. Ignoring other considerations, the best detector design is the one with the most detecting volume. However, operational limitations guide a) the maximum acceptable size of each detector cell (due to PSD performance and maximum-acceptable per-channel data throughput rates, limited by pulse pile-up and the processing rate of the electronics components of the system) and b) the affordability of a system due to the number of total channels of data to be collected and processed. As a first estimate, it appears that a system comprised of two rows of detectors 5" Ø $\times$ 3" would yield a working prototype system with excellent performance capabilities for assaying Pu-containing items and capable of handling high signal rates likely when measuring items with Pu and other actinides. However, it is still likely that gamma-ray shielding will be needed to reduce the total signal rate in the detectors. As a first step prior to working with these larger-sized detectors, it may be practical to perform scoping studies using small detectors, such as already-on-hand 3" Ø $\times$ 3" detectors.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

Neutron Detector Signal Processing to Calculate the Effective Neutron Multiplication Factor of Subcritical Assemblies

This report describes different methodologies to calculate the effective neutron multiplication factor of subcritical assemblies by processing the neutron detector signals using MATLAB scripts. The subcritical assembly can be driven either by a spontaneous fission neutron source (e.g. californium) or by a neutron source generated from the interactions of accelerated particles with target materials. In the latter case, when the particle accelerator operates in a pulsed mode, the signals are typically stored into two files. One file contains the time when neutron reactions occur and the other contains the times when the neutron pulses start. In both files, the time is given by an integer representing the number of time bins since the start of the counting. These signal files are used to construct the neutron count distribution from a single neutron pulse. The built-in functions of MATLAB are used to calculate the effective neutron multiplication factor through the application of the prompt decay fitting or the area method to the neutron count distribution. If the subcritical assembly is driven by a spontaneous fission neutron source, then the effective multiplication factor can be evaluated either using the prompt neutron decay constant obtained from Rossi or Feynman distributions or the Modified Source Multiplication (MSM) method.

73 NUCLEAR PHYSICS AND RADIATION PHYSICS↗

MicroRNAs as disease progression biomarkers and therapeutic targets in experimental autoimmune encephalomyelitis model of multiple sclerosis

Multiple sclerosis is an autoimmune neurodegenerative disease of the central nervous system characterized by pronounced inflammatory infiltrates entering the brain, spinal cord and optic nerve leading to demyelination. Focal demyelination is associated with relapsing-remitting multiple sclerosis, while progressive forms of the disease show axonal degeneration and neuronal loss. The tests currently used in the clinical diagnosis and management of multiple sclerosis have limitations due to specificity and sensitivity. MicroRNAs (miRNAs) are dysregulated in many diseases and disorders including demyelinating and neuroinflammatory diseases. A review of recent studies with the experimental autoimmune encephalomyelitis animal model (mostly female mice 6–12 weeks of age) has confirmed miRNAs as biomarkers of experimental autoimmune encephalomyelitis disease and importantly at the pre-onset (asymptomatic) stage when assessed in blood plasma and urine exosomes, and spinal cord tissue. The expression of certain miRNAs was also dysregulated at the onset and peak of disease in blood plasma and urine exosomes, brain and spinal cord tissue, and at the post-peak (chronic) stage of experimental autoimmune encephalomyelitis disease in spinal cord tissue. Therapies using miRNA mimics or inhibitors were found to delay the induction and alleviate the severity of experimental autoimmune encephalomyelitis disease. Interestingly, experimental autoimmune encephalomyelitis disease severity was reduced by overexpression of miR-146a, miR-23b, miR-497, miR-26a, and miR-20b, or by suppression of miR-182, miR-181c, miR-223, miR-155, and miR873. Further studies are warranted on determining more fully miRNA profiles in blood plasma and urine exosomes of experimental autoimmune encephalomyelitis animals since they could serve as biomarkers of asymptomatic multiple sclerosis and disease course. Additionally, studies should be performed with male mice of a similar age, and with aged male and female mice.

59 BASIC BIOLOGICAL SCIENCES↗