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At least 469 records · Page 26

Determination of a suitable molar absorption coefficient (ε) for lignin analysis of fibrous plants using the CASA method

In this study, the benchmarking and applicability of the CASA method for the analysis of fibrous plants, such as flax, hemp, and jute, is evaluated. Lignin is a phenolic biopolymer present in plant cell walls and is composed of three primary monomeric units, designated G, S, and H. Various factors, such as the genetic variability, influence the relative proportions of these units in plant samples. Recently, the cysteine-assisted sulfuric acid (CASA) method has been introduced as a rapid method for the quantification of lignin in wood samples. The aim of this study is to establish a suitable molar absorption coefficient (ε, L·g –1 ·cm –1 ) to adapt the CASA method for use with annual plant fibers. This investigation was motivated by the technical advantages of the CASA method, including higher throughput, lower reaction temperatures, and ecological benefits due to the absence of carcinogenic, mutagenic, or reprotoxic (CMR) substances and the need for minimal sample quantities. In this method, lignin solubilization is facilitated by cysteine, which is an amino acid that enhances the reaction kinetics, using a one-hour incubation period. However, as with any spectrophotometric technique, the CASA method depends on a molar absorption coefficient (ε) that varies according to the ratio of the aromatic units within the polymer. To evaluate the suitability of CASA for quantifying lignin in economically significant plant fibers, we investigate the impact of the unit ratios on the accuracy of ε. The results are compared with those of two widely recognized lignin analytical methods: the Klason method, which is a gravimetric reference method, and the acetyl bromide soluble lignin method, which is the most commonly used spectrophotometric approach. The final ε obtained in this study reveals a relative difference in lignin content ranging from –8 % to +9 % based on a comparison between the CASA and Klason methods across different industrial hemp varieties. As a result, using known G:S ratios in annual fibrous plants, ε can be estimated from our results.

59 BASIC BIOLOGICAL SCIENCES↗

The Integrated Impact of Diet on Human Immune Response, the Gut Microbiota, and Nutritional Status During Adaptation to a Spaceflight Analog

Spaceflight impacts human physiology, including well documented immune system dysregulation. Diet, immune function, and the microbiome are interlinked, but diet is the only one of these factors that we have the ability to easily, and significantly, alter on Earth or during flight. As we understand dietary impacts on physiology more thoroughly, we may then improve the spaceflight diet to improve crew health and potentially reduce spaceflight-associated physiological alterations. It is expected that increasing the consumption of fruits and vegetables and bioactive compounds (e.g., omega-3 fatty acids, lycopene, flavonoids) and therefore enhancing overall nutritional intake from the nominal shelf-stable, fully-processed space food system could serve as a countermeasure to improve human immunological profiles, the taxonomic profile of the gut microbiota, and nutritional status, especially where currently dysregulated during spaceflight. This interdisciplinary study will determine the effect of the current shelf-stable spaceflight diet compared to an "enhanced" shelf-stable spaceflight diet (25% more foods rich in omega-3 fatty acids, lycopene, flavonoids, and more fruits, and vegetables in general). The NASA Human Exploration Research Analog (HERA) 2017 missions, consisting of four 45-day missions with closed chamber confinement and realistic mission simulation in a high-fidelity mock space vehicle, will serve as a platform to replicate mission stressors and the effects on crew biochemistry, immunology, and the gut microbiome. Bio sampling of crewmembers is scheduled for selected intervals pre- and in-mission. Data collection also includes dietary intake recording. Outcome measures will include immune markers (e.g., peripheral leukocyte distribution, inflammatory cytokine profiles, T cell function), the taxonomic and metatranscriptomic profile of the gut microbiome, and nutritional status biomarkers and metabolites. Statistical evaluations will determine physiological and biochemical shifts in relation to nutrient intake and study phase. To date, sample collection has been completed for 2 crewmembers from the first mission, aka Campaign 4 Mission 1. Mission 2 was terminated after 22 days due to effects of Hurricane Harvey, and sample collection was not completed. Sample collection will continue for Campaign 4 Mission 3 and 4 prior to comprehensive sample analysis. Beneficial improvements will provide evidence of the impact of diet on crew health and adaptation to this spaceflight analog, and will aid in the design and development of more-efficient targeted dietary interventions for exploration missions.

Douglas, G. L.↗

Principles, Techniques, and Applications of Tissue Microfluidics

The principle of tissue microfluidics and its resultant techniques has been applied to cell analysis. Building microfluidics to suit a particular tissue sample would allow the rapid, reliable, inexpensive, highly parallelized, selective extraction of chosen regions of tissue for purposes of further biochemical analysis. Furthermore, the applicability of the techniques ranges beyond the described pathology application. For example, they would also allow the posing and successful answering of new sets of questions in many areas of fundamental research. The proposed integration of microfluidic techniques and tissue slice samples is called tissue microfluidics because it molds the microfluidic architectures in accordance with each particular structure of each specific tissue sample. Thus, microfluidics can be built around the tissues, following the tissue structure, or alternatively, the microfluidics can be adapted to the specific geometry of particular tissues. By contrast, the traditional approach is that microfluidic devices are structured in accordance with engineering considerations, while the biological components in applied devices are forced to comply with these engineering presets. The proposed principles represent a paradigm shift in microfluidic technology in three important ways: Microfluidic devices are to be directly integrated with, onto, or around tissue samples, in contrast to the conventional method of off-chip sample extraction followed by sample insertion in microfluidic devices. Architectural and operational principles of microfluidic devices are to be subordinated to suit specific tissue structure and needs, in contrast to the conventional method of building devices according to fluidic function alone and without regard to tissue structure. Sample acquisition from tissue is to be performed on-chip and is to be integrated with the diagnostic measurement within the same device, in contrast to the conventional method of off-chip sample prep and subsequent insertion into a diagnostic device. A more advanced form of tissue integration with microfluidics is tissue encapsulation, wherein the sample is completely encapsulated within a microfluidic device, to allow for full surface access. The immediate applications of these approaches lie with diagnostics of tissue slices and biopsy samples e.g. for cancer but the approaches would also be very useful in comparative genomics and other areas of fundamental research involving heterogeneous tissue samples.

Wade, Lawrence A.↗

Kinetic analysis of Cas12a and Cas13a RNA-Guided nucleases for development of improved CRISPR-Based diagnostics

Bacterial CRISPR systems provide acquired immunity against invading nucleic acids by activating RNA-programmable RNases and DNases. Cas13a and Cas12a enzymes bound to CRISPR RNA (crRNA) recognize specific nucleic acid targets, initiating cleavage of the targets as well as non-target (trans) nucleic acids. Here, we examine the kinetics of single-turnover target and multi-turnover trans-nuclease activities of both enzymes. High-turnover, non-specific Cas13a trans-RNase activity is coupled to rapid binding of target RNA. By contrast, low-turnover Cas12a trans-nuclease activity is coupled to relatively slow cleavage of target DNA, selective for DNA over RNA, indifferent to base identity, and preferential for single-stranded substrates. Combining multiple crRNA increases detection sensitivity of targets, an approach we use to quantify pathogen DNA in samples from patients suspected of Buruli ulcer disease. Results reveal that these enzymes are kinetically adapted to play distinct roles in bacterial adaptive immunity and show how kinetic analysis can be applied to CRISPR-based diagnostics.

59 BASIC BIOLOGICAL SCIENCES↗

Improving the Parameterization of Cloud and Rain Microphysics in E3SM using Novel Observationally-Constrained Bayesian Approach (Final Technical Report)

In this project, we sought to develop new cloud and rain microphysics frameworks within the Energy Exascale Earth System Model (E3SM). This work encompassed two primary avenues of research: 1) Further development of a Bayesian-based scheme called BOSS (Bayesian Observationally-constrained Statistical-physical Scheme) to represent cloud and rain microphysics, testing it in realistic high-resolution cloud models, and implementing it in E3SM; 2) Development of a methodology utilizing machine learning to enable computationally tractable use of tractable use of Markov chain Monte Carlo sampling for Bayesian parameter estimation in Earth system and cloud models. In this project, we adapted the BOSS microphysics scheme, originally formulated for rain-only, to include all liquid-phase microphysical processes for cloud and rain, in particular the processes that mediate between these two categories, for example the conversion from cloud to rain through collision and coalescence of drops. We constrained the scheme via comparison and testing against a detailed model that explicitly represents the evolution of cloud and rain particles, called a bin microphysics scheme.

54 ENVIRONMENTAL SCIENCES↗

An Efficient Implementation of Forward-Backward LMS Adaptive Line Enhancer

An efficient implementation of the forward-backward least mean square (FBLMS) adaptive line enhancer is presented. Without changing the characteristics of the FBLMS adaptive line enhancer, the proposed implementation technique reduces multiplications by 25 percent and additions by 12.5 percent in two successive time samples in comparison to that of the direct implementation in both prediction and weight control. It can be applied to acquisition and tracking in both stationary and nonstationary environments.

adaptive line enhancers signal acquisition trackin↗

Tactile Sensing for Dexterous Robotic Hands

Robotic systems will be used as precursors to human exploration to explore the solar system and expand our knowledge of planetary surfaces. Robotic systems will also be used to build habitats and infrastructure required for human presence in space and on other planetary surfaces . Such robots will require a high level of intelligence and automation. The ability to flexibly manipulate their physical environment is one characteristic that makes humans so effective at these building and exploring tasks . The development of a generic autonomous grasp ing capability will greatly enhance the efficiency and ability of robotics to build, maintain and explore. To tele-operate a robot over vast distances of space, with long communication delays, has proven to be troublesome. Having an autonomous grasping capability that can react in real-time to disturbances or adapt to generic objects, without operator intervention, will reduce the probability of mishandled tools and samples and reduce the number of re-grasp attempts due to dropping. One aspect that separates humans from machines is a rich sensor set. We have the ability to feel objects and respond to forces and textures. The development of touch or tactile sensors for use on a robot that emulates human skin and nerves is the basis for this discussion. We will discuss the use of new piezo-electric and resistive materials that have emerged on the market with the intention of developing a touch sensitive sensor. With viable tacti le sensors we will be one step closer to developing an autonomous grasping capability.

Martin, Toby B.↗

Developing compact tuning fork thermometers for sub-mK temperatures and high magnetic fields

There is a growing demand for experiments on calorimetric and thermal transport measurements at ultra-low temperatures below 1 mK and high magnetic fields up to 16 T. Particularly, milligram-sized solid samples are of great interest. Here, we present the development of scalable thermometers based on quartz tuning fork resonators immersed in liquid 3 He and adapt hydrodynamic models to provide an improved description of temperature dependence in the high viscosity regime between 1 and 10mK. We demonstrate successful thermometer operation and discuss the feasibility of fast and compact thermal probes suitable for small samples.

47 OTHER INSTRUMENTATION↗

Precision Local Burnup Assessment Through Dynamic Peak Fitting in Atom Probe Tomography for Depleted, Enriched, and Irradiated Metallic and Ceramic Fuels

Abstract Burnup estimation in nuclear fuels is vital for evaluating fuel performance, transportation, and safe fuel storage. Accurate assessments of burnup from service period and spent fuels involve tracking the consumption of fissile isotopes of uranium (U) offering a direct insight into energy changes within the fuels especially for thermal spectrum reactors. In current approach, mass spectroscopic technique in atom probe tomography (APT) is utilized for accurate quantification of U isotopes. Quantification of U peaks in mass spectrum is performed on asymmetric shapes due to delayed signals, known as thermal tails, particularly for poorly conducting samples analyzed in laser mode. In this study, we introduce a novel quantification tool for isotopic analysis from APT datasets by developing a fitting algorithm based on shapes of the peaks. A MATLAB-based dynamic peak fitting toolbox is developed and designed to adapt to various peak shapes, ensuring accurate quantification of U isotopes. The effectiveness of this approach is demonstrated in standard Ni-Cr sample, depleted and enriched U samples, and U-based fuels with different burnup levels. The viability of this approach for isotopic quantification is demonstrated on both metallic and ceramic fuels.

Burnup↗

Fungal diversity and function in metagenomes sequenced from extreme environments

Fungi are increasingly recognized as key players in various extreme environments. Here we present an analysis of publicly-sourced metagenomes from global extreme environments, focusing on fungal taxonomy and function. The majority of 855 selected metagenomes contained scaffolds assigned to fungi. Relative abundance of fungi was as high as 10% of protein-coding genes with taxonomic annotation, with up to 289 fungal genera per sample. Despite taxonomic clustering by environment, fungal communities were more dissimilar than archaeal and bacterial communities, both for within- and between-environment comparisons. Relatively abundant fungal classes in extreme environments included Dothideomycetes, Eurotiomycetes, Leotiomycetes, Pezizomycetes, Saccharomycetes, and Sordariomycetes. Broad generalists and prolific aerial spore formers were the most relatively abundant fungal genera detected in most of the extreme environments, bringing up the question of whether they are actively growing in those environments or just surviving as spores. More specialized fungi were common in some environments, such as zoosporic taxa in cryosphere water and hot springs. Relative abundances of genes involved in adaptation to general, thermal, oxidative, and osmotic stress were greatest in soda lake, acid mine drainage, and cryosphere water samples.

60 APPLIED LIFE SCIENCES↗

Meissner-London Susceptibility of Superconducting Right Circular Cylinders in an Axial Magnetic Field

Analysis of magnetic susceptibility of nonellipsoidal samples is a long-standing problem in experimental studies of magnetism and superconductivity. Here the quantitative description of the Meissner-London response (no Abrikosov vortices) of right circular cylinders in an axial magnetic field is given. Three-dimensional adaptive finite-element modeling is used to calculate the total magnetic moment ( m ) in a wide range of London penetration depth ( λ ) to sample size ( a ) ratios, λ /a. Furthermore, by fitting numerical solutions, the closed-form empirical magnetic susceptibility is obtained involving only sample dimensions and λ, thus providing a recipe for determining the London penetration depth from the precision measurements of the magnetic susceptibility. Detailed protocols of the experimental data analysis using the developed approach are given. The results can be readily extended to the most frequently used cuboid-shaped samples.

3-dimensional systems↗

Adaptive control strategies for cooperative dual-arm manipulators

Three strategies for adaptive control of cooperative dual-arm robots are discussed. Implementation of these adaptive controllers does not require the use of complex mathematical models of the arm dynamics or knowledge of the arm dynamic parameters or load parameters. These strategies have simple structures, and are computationally fast for on-line implementation with high sampling rates. In all three cases, the coupling effects between the arms through the load are treated as disturbances which are rejected by the adaptive controllers while following desired commands in a common frame of reference. Simulation results demonstrate the usefulness of the controllers.

Seraji, H.↗

Mercury Speciation of Hanford 241-AP-107 Tank Waste Samples - 20384

The concentration of key mercury species in the Hanford tank waste has not been previously measured. Based on process knowledge, an estimated inventory of 2,100 kg of mercury is assumed and was distributed in varied amounts across 149 single-shell tanks (SSTs) and 28 double-shell tanks (DSTs). Limited analyses of individual SSTs and DSTs for total mercury were all below detection, yielding upper limits in concentration estimates based solely on instrument detection limits, resulting in an uncertain mercury inventory for Hanford tanks. Accurate and reliable chemical speciation and quantification is desired to anticipate, predict, and abate the mass movements of various mercury species through the Hanford waste processing flowsheet. Highly sensitive separation and quantification methods for total, elemental, and monomethyl mercury species, in tank waste, have been developed at the Pacific Northwest National Laboratory's (PNNL) Radiochemical Processing Laboratory (RPL). A non-radiological environmental method to quantify mercury species has been adapted for application to Hanford tank 241-AP-107 raw supernatant feed, filtered feed, and cesium-decontaminated samples. These samples represent feed inventory and pretreatment process effluents prior to immobilization by vitrification. This method separates and preconcentrates total, elemental, and monomethyl mercury onto selective solid substrates with little-to-no radioactive background. The solid samples can then be released from strict radiological control, allowing for analytical work to be conducted in low-level radiological facilities. Separation of mercury species was demonstrated at PNNL's RPL in Richland, Washington before transport and analytical quantification using cold vapor atomic fluorescence spectrometry (CVAFS) at PNNL's Marine Science Laboratory (MSL) in Sequim, Washington. Additional studies were conducted to assess the stability of monomethyl mercury in distilled matrices and preconcentrated onto the Carbotrap{sup R} solid substrate to assess sample degradation over time during transport. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

Maneuver Design and Calibration for the Genesis Spacecraft

Genesis is the fifth mission selected as part of NASA's Discovery Program. The objective of Genesis is to collect solar wind samples for a period of approximately two years while in a halo orbit about the Earth-Sun L I point. At the end of this period, the samples are to be returned to a specific recovery point on the Earth for subsequent analysis. This goal has never been attempted before and presents a formidable challenge in terms of mission design and operations, particularly planning and execution of propulsive maneuvers. To achieve a level of cost-effectiveness consistent with a Discovery-class mission, the Genesis spacecraft design was adapted to the maximum extent possible from designs used on earlier missions, such as Mars Global Surveyor (MGS) and Stardust, another sample collection mission. The spacecraft design for Genesis is shown. Spin stabilization was chosen for attitude control, in lieu of three-axis stabilization, with neither reaction wheels nor accelerometers included. This precludes closed-loop control of propulsive maneuvers and implies that any attitude changes, including spin changes and precessions, will behave like translational propulsive maneuvers and affect the spacecraft trajectory. Moreover, to minimize contamination risk to the samples collected, all thrusters were placed on the side opposite the sample collection canister. The orientation and characteristics of thrusters are indicated. For large maneuvers (>2.5 m/s), two 5 lbf thrusters will be used for delta v, with precession to the burn attitude, followed by spin-up from 1.6 to 10 rpm before the burn and spin down to 1.6 rpm afterwards, then precession back to the original spin attitude. For small maneuvers (<2.5 m/s), no spin change is needed and four 0.2 lbf thrusters are used for Av. Single or double 360 deg. precession changes are required whenever the desired delta v falls inside the two-way turn circle (about 0.4 m/s) based on the mass properties, spin rate and lever arm lengths based on thruster locations. In such instances, delta v resulting from spacecraft precession cannot be used effectively as a component of the desired delta v, and must therefore be removed by precessing at least one complete revolution around the turn circle. To eliminate cross-track execution errors, a second revolution in the opposite direction would also be performed. This paper will address the design of propulsive maneuvers in light of the aforementioned challenges and other constraints. Maneuver design will be performed jointly by the Navigation Team at JPL and the Spacecraft Team at LMA, based on the process indicated . Typical maneuver timelines will be presented which address considerations introduced by attitude changes. These include nutation, which is introduced by precessing or spinning down and must be given sufficient time to damp out prior to execution of subsequent events, as well as sun and earth pointing constraints, which must be considered to ensure sufficient spacecraft power and to minimize telecommunications interruptions, respectively. The paper will include a description of how individual propulsive maneuvers are resolved into components to account for delta v from translational burns and spacecraft attitude changes required to carry out such maneuvers. Contributions to maneuver delta v arising from attitude changes, based on mass properties for the period just after launch, are indicated. Similar curves will be presented spanning all mission phases from launch through return. A set of closed-form equations for resolving maneuver components, base on a specific delta v required for correction or deterministic changes to the spacecraft trajectory will be presented, as well. In addition to nominal maneuvers, special calibration maneuvers are planned to improve open-loop modeling of maneuvers and to reduce execution errors. Uncalibrated execution errors are indicated. Such errors could be reduced by 50% or more over the course of the mission. Special calibrations are of particular importance for the return leg of the mission, since the sample canister must be returned to a specific location within the Utah Test and Training Range (UTTR) for mid-air retrieval. An entry angle tolerance of no less than +/- 0.08 deg. is required to achieve this objective. Biasing of the final return maneuvers coupled with a specific maneuver mode to use a series of well-characterized spin changes to effect these maneuvers is part of the current Genesis baseline mission plan. Another important objective of calibrations is to better characterize precession maneuvers. Such maneuvers are part of most propulsive maneuvers, but are also required periodically to maintain sun-pointing for power or daily during solar-wind pointing during collection periods. Although relatively small, such maneuvers will have a significant cumulative impact on orbit determination, particularly in the halo portion of the mission. The current mission design also calls for three stationkeeping maneuvers during each halo orbit of approximately six months duration. These stationkeeping maneuvers may be sufficiently small that single or double 360 deg. precession changes may be required. Because there are no accelerometers on board the spacecraft, calibration can only be performed with the aid of ground-based radiometric tracking. To establish a high degree of accuracy in characterizing the magnitude of burns, the spacecraft spin axis should be along the line of sight to the Earth, providing Doppler measurements with <1 mm/sec accuracy in S-Band. Emission constraints allow such alignment only during certain portions of the mission when the Earth-spacecraft-sun geometry is favorable. The impact of precessions, or burns at times when geometry is not favorable, can be assessed by reconstruction of the spacecraft trajectory using tracking arcs of several days before and after the event.

Williams, Kenneth E.↗

HBMax: Optimizing Memory Efficiency for Parallel Influence Maximization on Multicore Architectures

The goal of influence maximization is to select k most-influential vertices or seeds in a network, where influence is defined by a given diffusion process. The problem has a number of important applications such as viral marketing, information spread, and epidemic control. Although computing optimal seed set is NP-Hard, due to the submodular nature of the problem efficient approximation algorithms exist. However, even state-of-the-art parallel implementations are limited by a sampling step that incurs large memory footprints. This in turn limits the problem size reach and approximation quality. In this work, we study the memory footprint of the sampling process collecting reverse reachability information in the IMM algorithm over large real-world social networks. We present an adaptive and memory-efficient optimization approach for a state-of-the-art multi-threaded parallel influence maximization algorithm. Our approach,HuffMax, uses a portion of the reverse reachable (RR) sets collected by the algorithm to learn the characteristics of the graph. Then, it compresses the intermediate reverse reachability information with Huffman coding, and queries directly on the compressed data to preserve the memory savings obtained through compression. We also propose an efficient sampling strategy based on the distribution of RR sets, which can further reduce the computation time for typical social networks with long-tail distributions. Considering a NUMA architecture, we scale up our solution on 128-core CPUs and reduce the memory footprint by up to 45.7% with negligible time overhead (or even faster) and without perceivable loss of accuracy.

Chen, Xinyu↗

Robotic sampling system for an unmanned Mars mission

A major robotics opportunity for NASA will be the Mars Rover/Sample Return Mission which could be launched as early as the 1990s. The exploratory portion of this mission will include two autonomous subsystems: the rover vehicle and a sample handling system. The sample handling system is the key to the process of collecting Martian soils. This system could include a core drill, a general-purpose manipulator, tools, containers, a return canister, certification hardware and a labeling system. Integrated into a functional package, the sample handling system is analogous to a complex robotic workcell. Discussed here are the different components of the system, their interfaces, forseeable problem areas and many options based on the scientific goals of the mission. The various interfaces in the sample handling process (component to component and handling system to rover) will be a major engineering effort. Two critical evaluation criteria that will be imposed on the system are flexibility and reliability. It needs to be flexible enough to adapt to different scenarios and environments and acquire the most desirable specimens for return to Earth. Scientists may decide to change the distribution and ratio of core samples to rock samples in the canister. The long distance and duration of this planetary mission places a reliability burden on the hardware. The communication time delay between Earth and Mars minimizes operator interaction (teleoperation, supervisory modes) with the sample handler. An intelligent system will be required to plan the actions, make sample choices, interpret sensor inputs, and query unknown surroundings. A combination of autonomous functions and supervised movements will be integrated into the sample handling system.

Chun, Wendell↗