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At least 451 records · Page 25

Biological Sterilization of Returned Mars Samples

Martian rock and soil, collected by robotic spacecraft, will be returned to terrestrial laboratories early in the next century. Current plans call for the samples to be immediately placed into biological containment and tested for signs of present or past life and biological hazards. It is recommended that "Controlled distribution of unsterilized materials from Mars should occur only if rigorous analyses determine that the materials do not constitute a biological hazard. If any portion of the sample is removed from containment prior to completion of these analyses it should first be sterilized." While sterilization of Mars samples may not be required, an acceptable method must be available before the samples are returned to Earth. The sterilization method should be capable of destroying a wide range of organisms with minimal effects on the geologic samples. A variety of biological sterilization techniques and materials are currently in use, including dry heat, high pressure steam, gases, plasmas and ionizing radiation. Gamma radiation is routinely used to inactivate viruses and destroy bacteria in medical research. Many commercial sterilizers use Co-60 , which emits gamma photons of 1.17 and 1.33 MeV. Absorbed doses of approximately 1 Mrad (10(exp 8) ergs/g) destroy most bacteria. This study investigates the effects of lethal doses of Co-60 gamma radiation on materials similar to those anticipated to be returned from Mars. The goals are to determine the gamma dose required to kill microorganisms in rock and soil samples and to determine the effects of gamma sterilization on the samples' isotopic, chemical and physical properties. Additional information is contained in the original extended abstract.

Allen, C. C.↗

A New Compression Method for FITS Tables

As the size and number of FITS binary tables generated by astronomical observatories increases, so does the need for a more efficient compression method to reduce the amount disk space and network bandwidth required to archive and down1oad the data tables. We have developed a new compression method for FITS binary tables that is modeled after the FITS tiled-image compression compression convention that has been in use for the past decade. Tests of this new method on a sample of FITS binary tables from a variety of current missions show that on average this new compression technique saves about 50% more disk space than when simply compressing the whole FITS file with gzip. Other advantages of this method are (1) the compressed FITS table is itself a valid FITS table, (2) the FITS headers remain uncompressed, thus allowing rapid read and write access to the keyword values, and (3) in the common case where the FITS file contains multiple tables, each table is compressed separately and may be accessed without having to uncompress the whole file.

Pence, William↗

Objective estimates based on experimental data and initial and final knowledge

An extension of the method of Jaynes, whereby least biased probability estimates are obtained, permits such estimates to be made which account for experimental data on hand as well as prior and posterior knowledge. These estimates can be made for both discrete and continuous sample spaces. The method allows a simple interpretation of Laplace's two rules: the principle of insufficient reason and the rule of succession. Several examples are analyzed by way of illustration.

Rosenbaum, B. M.↗

Reflector Surface Error Compensation in Dual-Reflector Antennas

By probing the field on a small subreflector at a minimal number of points, the main reflector surface errors can be obtained and subsequently used to design a phase-correction subreflector that can compensate for main reflector errors. The compensating phase-error profile across the subreflector can be achieved either by a surface deformation or by the use of an array of elements such as patch antennas that can cause a phase shift between the incoming and outgoing fields. The second option is of primary interest here, but the methodology can be applied to either case. The patch array is most easily implemented on a planar surface. Therefore, the example of a flat subreflector and a parabolic main reflector (a Newtonian dual reflector system) is considered in this work. The subreflector is assumed to be a reflector array covered with patch elements. The phase variation on a subreflector can be detected by a small number of receiving patch elements (probes). By probing the phase change at these few selected positions on the subreflector, the phase error over the entire surface can be recovered and used to change the phase of all the patch elements covering the subreflector plane to compensate for main reflector errors. This is accomplished by using a version of sampling theorem on the circular aperture. The sampling is performed on the phase-error function on the circular aperture of the main reflector by a method developed using Zernike polynomials. This method is based upon and extended from a theory previously proposed and applied to reflector aperture integration. This sampling method provides for an exact retrieval of the coefficients of up to certain orders in the expansion of the phase function, from values on a specifically calculated set of points in radial and azimuthal directions in the polar coordinate system, on the circular reflector aperture. The corresponding points on the subreflector are then obtained and, by probing the fields at these points, a set of phase values is determined that is then transferred back to the main reflector aperture for recovering the phase function. Once this function is recovered, the corresponding phase function on the subreflector is calculated and used to compensate for main reflector surface errors. In going back and forth between sub and main reflectors, geometrical (ray) optics is employed, which even though it ignores edge diffraction and other effects, is shown to be accurate for phase recovery.

Jamnejad, Vahraz↗

A PCR Based Microbial Monitoring Alternative Method of Detection and Identification of Microbes Aboard ISS

Previous research has shown that microorganisms and potential human pathogens have been detected on the International Space Station (ISS) with additional introduction of new microflora occurring with every exchange of crew or addition of equipment and supplies. These microbes are readily transferred between crew and subsystems (i.e. ECLSS, environmental control and life support systems). As this can be detrimental to astronaut health and optimal performance of ISS systems, monitoring of systems such as ECLSS to include identification of microbial contaminants could prevent adverse effects on human health and life support systems. Current monitoring on ISS is laborious and utilizes culture based methods followed by sample return to Earth for complete analysis. Future, long-distance spaceflight missions will require real-time monitoring capabilities that enable efficient and rapid assessments of the microbial environment allowing for expedited decisions and more targeted response to cope with anomalies. Polymerase chain reaction (PCR), a molecular microbial monitoring method was chosen and numerous PCR instruments investigated for their potential to perform in microgravity conditions. Using ISS as a test bed for PCR verification in microgravity will enable NASA to assess whether molecular based microbiological sensors may be components of reliable, closed-loop life support and habitation systems in spacecraft, enhancing infrastructure capabilities through increased efficiency, reliability, and time savings by enabling sample analysis on orbit. NASA selected the Water Monitoring Suite as one of the rapid spaceflight hardware demonstration activities utilizing a streamlined process to minimize the time required to fly experimental flight hardware. The RAZOR EX (BioFire Defense, Salt Lake City, UT) system was part of the water monitoring suite and is a commercial off-the-shelf (COTS) real-time PCR instrument designed for field work. The RAZOR EX was originally designed for Department of Defense (DoD) under a small business innovative research (SBIR) grant and is ruggedized, compact and provides a rapid, sample to answer in less than an hour. PCR assays using a fluorescent probe were optimized and spiked with known concentrations of DNA (Pseudomonas aeruginosa) ranging from 0.002 to 20 ng. PCR reagents were lyophilized and configured in customized pouches and tested for flight readiness. Three types of water were used to rehydrate the reagents and demonstrate the fidelity of the PCR reaction in microgravity. Molecular grade deionized water served as a control while filtered and unfiltered ISS potable water served to test for chemical or biological inhibitors. All three types were compared to parallel ground test results. Nine tests were run on ISS (3 of each water type) and the critical threshold cycle (Ct) was compared to parallel ground tests completed at Kennedy Space Center, FL and Johnson Space Center, TX. All concentrations of Pseudomonas aeruginosa DNA were detected. A comparison of the Ct produced in real time PCR indicated similarity between flight and ground samples. There appeared to be no significant difference between flight or ground PCR reactions or between any of the three water types. This testing demonstrated the ability to perform molecular testing during spaceflight operations with similar sensitivity. It will allow for future ground development of molecular protocols and minimize the need for spaceflight testing. Future testing will include development of additional targets including environmental and health related organisms.

Christina Khodadad↗

Learning process mapping heuristics under stochastic sampling overheads

A statistical method was developed previously for improving process mapping heuristics. The method systematically explores the space of possible heuristics under a specified time constraint. Its goal is to get the best possible heuristics while trading between the solution quality of the process mapping heuristics and their execution time. The statistical selection method is extended to take into consideration the variations in the amount of time used to evaluate heuristics on a problem instance. The improvement in performance is presented using the more realistic assumption along with some methods that alleviate the additional complexity.

Ieumwananonthachai, Arthur↗

Apparatus and method for centrifugation and robotic manipulation of samples

A device for centrifugation and robotic manipulation of specimen samples, including incubating eggs, and uses thereof are provided. The device may advantageously be used for the incubation of avian, reptilian or any type of vertebrate eggs. The apparatus comprises a mechanism for holding samples individually, rotating them individually, rotating them on a centrifuge collectively, injecting them individually with a fixative or other chemical reagent, and maintaining them at controlled temperature, relative humidity and atmospheric composition. The device is applicable to experiments involving entities other than eggs, such as invertebrate specimens, plants, microorganisms and molecular systems.

Vellinger, John C.↗

Multi Model Monte Carlo with Python (MXMCPy)

Multi Model Monte Carlo with Python (\mxmc {}) is a software package developed as a general capability for computing the statistics of outputs from an expensive, high-fidelity model by leveraging faster, low-fidelity models for speedup. Motivated by uncertainty propagation problems where classical Monte Carlo (MC) simulation is computationally intractable, various multi-model MC approaches have recently emerged that yield unbiased estimators with significantly reduced variance relative to MC for the same cost. These existing methods include multi-level Monte Carlo (MLMC), multi-fidelity Monte Carlo (MFMC), and approximate control variates (ACV). Given a fixed computational budget and a collection of models with varying cost/accuracy, each method seeks a sample allocation strategy across the models that results in an estimator with optimal variance reduction. \mxmc {} is a versatile tool that enables convenient access to many existing multi-model MC approaches within one modular and extensible package. With \mxmc {}, users can easily compare existing methods to determine the best choice for their particular problem, while developers have a basis for implementing and sharing new variance reduction approaches. This report introduces the \mxmc {} software, providing a summary of the problem-solving workflow for users as well as a brief overview of the code layout for developers.

Geoffrey F Bomarito↗

Application of a Bayesian Framework for Plasticity Model Selection

Interpretable Machine Learning (IML) has performed well when tasked with deriving constitutive material models. However, IML has been shown to prefer models that overfit noise in data, which tends to lead to bloat and a decrease in interpretability. Due to these issues, the ability of IML to reliably derive models that fit the data and are both interpretable and generalizable is limited. A method developed recently has shown promise to improve upon traditional IML by using a Bayesian fitness definition for the evolution of free-form models with non-deterministic parameters. This framework was developed for genetic-programming-based symbolic regression(GPSR) and involves model parameter estimation using Sequential Monte Carlo sampling (SMC).The method has demonstrated a reduction in bloat when dealing with noisy data in comparison to conventional GPSR. The results of this framework applied to stress-strain data for copper show models that more effectively predict the experimental data better than was previously shown with GPSR.

plasticity↗

Lunar rilles: A catalog and method of classification

A representative sample of lunar rilles observed on Lunar Orbiter 4 and 5 photographs is presented. A method of classification of rilles is described which will provide a means of quantitatively grouping rilles of similar planemetric shape. The method is based on a finite Fourier approximation of the edge of the rille. It provides an adequate approximation to all planemetric characteristics of the rille except rille recurvature.

Oberbeck, V. R.↗

A remark on the theory of measuring thermal diffusivity by the modified Angstrom's method

A theory of the measurement of the thermal diffusivity of a sample by the modified Angstrom method is developed for the case in which radiative heat loss from the end surface of the sample is not negligible, and applied to measurements performed on lunar samples. Formulas allowing sample thermal diffusivity to be determined from the amplitude decay and phase lag of a temperature wave traveling through the sample are derived for a flat disk sample for which only heat loss from the end surface is important, and a sample of finite diameter and length for which heat loss through the end and side surfaces must be considered. It is noted that in the case of a flat disk, measurements at a single angular frequency of the temperature wave are sufficient, while the sample of finite diameter and length requires measurements at two discrete angular frequencies. Comparison of the values of the thermal diffusivities of two lunar samples of dimensions approximately 1 x 1 x 2 cm derived by the present methods and by the Angstrom theory for a finite bar reveals them to differ by not more than 5%, and indicates that more refined data are required as the measurement theory becomes more complicated.

Horai, K.-I.↗

Improved Compression of Wavelet-Transformed Images

A recently developed data-compression method is an adaptive technique for coding quantized wavelet-transformed data, nominally as part of a complete image-data compressor. Unlike some other approaches, this method admits a simple implementation and does not rely on the use of large code tables. A common data compression approach, particularly for images, is to perform a wavelet transform on the input data, and then losslessly compress a quantized version of the wavelet-transformed data. Under this compression approach, it is common for the quantized data to include long sequences, or runs, of zeros. The new coding method uses prefixfree codes for the nonnegative integers as part of an adaptive algorithm for compressing the quantized wavelet-transformed data by run-length coding. In the form of run-length coding used here, the data sequence to be encoded is parsed into strings consisting of some number (possibly 0) of zeros, followed by a nonzero value. The nonzero value and the length of the run of zeros are encoded. For a data stream that contains a sufficiently high frequency of zeros, this method is known to be more effective than using a single variable length code to encode each symbol. The specific prefix-free codes used are from two classes of variable-length codes: a class known as Golomb codes, and a class known as exponential-Golomb codes. The codes within each class are indexed by a single integer parameter. The present method uses exponential-Golomb codes for the lengths of the runs of zeros, and Golomb codes for the nonzero values. The code parameters within each code class are determined adaptively on the fly as compression proceeds, on the basis of statistics from previously encoded values. In particular, a simple adaptive method has been devised to select the parameter identifying the particular exponential-Golomb code to use. The method tracks the average number of bits used to encode recent runlengths, and takes the difference between this average length and the code parameter. When this difference falls outside a fixed range, the code parameter is updated (increased or decreased). The Golomb code parameter is selected based on the average magnitude of recently encoded nonzero samples. The coding method requires no floating- point operations, and more readily adapts to local statistics than other methods. The method can also accommodate arbitrarily large input values and arbitrarily long runs of zeros. In practice, this means that changes in the dynamic range or size of the input data set would not require a change to the compressor. The algorithm has been tested in computational experiments on test images. A comparison with a previously developed algorithm that uses large code tables (generated via Huffman coding on training data) suggests that the data-compression effectiveness of the present algorithm is comparable to the best performance achievable by the previously developed algorithm.

Kiely, Aaron↗

Options for Robust Airfoil Optimization under Uncertainty

A robust optimization method is developed to overcome point-optimization at the sampled design points. This method combines the best features from several preliminary methods proposed by the authors and their colleagues. The robust airfoil shape optimization is a direct method for drag reduction over a given range of operating conditions and has three advantages: (1) it prevents severe degradation in the off-design performance by using a smart descent direction in each optimization iteration, (2) it uses a large number of spline control points as design variables yet the resulting airfoil shape does not need to be smoothed, and (3) it allows the user to make a tradeoff between the level of optimization and the amount of computing time consumed. For illustration purposes, the robust optimization method is used to solve a lift-constrained drag minimization problem for a two-dimensional (2-D) airfoil in Euler flow with 20 geometric design variables.

Padula, Sharon L.↗

New Computational Methods for the Prediction and Analysis of Helicopter Noise

This paper describes several new methods to predict and analyze rotorcraft noise. These methods are: 1) a combined computational fluid dynamics and Kirchhoff scheme for far-field noise predictions, 2) parallel computer implementation of the Kirchhoff integrations, 3) audio and visual rendering of the computed acoustic predictions over large far-field regions, and 4) acoustic tracebacks to the Kirchhoff surface to pinpoint the sources of the rotor noise. The paper describes each method and presents sample results for three test cases. The first case consists of in-plane high-speed impulsive noise and the other two cases show idealized parallel and oblique blade-vortex interactions. The computed results show good agreement with available experimental data but convey much more information about the far-field noise propagation. When taken together, these new analysis methods exploit the power of new computer technologies and offer the potential to significantly improve our prediction and understanding of rotorcraft noise.

Strawn, Roger C.↗

Determination of the isotopic composition of atmospheric methane and its application in the Antarctic

A procedure for establishing the C-13/C-12 ratio and the C-14 abundance in the atmospheric methane is discussed. The method involves air sample collection, measurement of the methane mixing ratio by gas chromotography followed by quantitative conversion of the methane in the air samples to CO2 and H2O, and analysis of the resulting CO2 for the C-13/C-12 ratio by stable isotope ratio mass spectrometry and measurement of C-14 content by accelerator mass spectrometry. The carbon isotropic composition of methane in air collected at Baring Head, New Zealand, and in air collected on aircraft flights between New Zealand and Antarctica is determined by the method, and no gradient in the composition between Baring Head and the South Pole station is found. As the technique is refined, and more data is gathered, small seasonal and long-term variations in C-13 are expected to be resolved.

Lowe, David C.↗

A New Approach for Checking and Complementing CALIPSO Lidar Calibration

We have been studying the backscatter ratio of the two CALIPSO wavelengths for 3 different targets. We are showing the ratio of integrate attenuated backscatter coefficient for cirrus clouds, ocean surface and liquid. Water clouds for one month of nightime data (left:July,right:December), Only opaque cirrus classified as randomly oriented ice[1] are used. For ocean and water clouds, only the clearest shots, determined by a threshold on integrated attenuated backscatter are used. Two things can be immediately observed: 1. A similar trend (black dotted line) is visible using all targets, the color ratio shows a tendency to be higher north and lower south for those two months. 2. The water clouds average value is around 15% lower than ocean surface and cirrus clouds. This is due to the different multiple scattering at 532 nm and 1064 nm [2] which strongly impact the water cloud retrieval. Conclusion: Different targets can be used to improve CALIPSO 1064 nm calibration accuracy. All of them show the signature of an instrumental calibration shift. Multiple scattering introduce a bias in liquid water cloud signal but it still compares very well with all other methods and should not be overlooked. The effect of multiple scattering in liquid and ice clouds will be the subject of future research. If there really is a sampling issue. Combining all methods to increase the sampling, mapping the calibration coefficient or trying to reach an orbit per orbit calibration seems an appropriate way.

Josset, Damien B.↗

Characterization of Carbon Dioxide Washout Measurement Techniques in the Mark-III Space Suit

It is essential to provide adequate carbon dioxide (CO2) washout in a space suit to reduce the risks associated with manned operations in space suits. Symptoms of elevated CO2 levels range from reduced cognitive performance and headache to unconsciousness and death at high levels of CO2. Because of this, NASA imposes limits on inspired CO2 levels for space suits when they are used in space and for ground testing. Testing and/or analysis must be performed to verify that a space suit meets CO2 washout requirements. Testing for developmental space suits has traditionally used an oronasal mask that collects CO2 samples at the left and rights sides of the mouth. Testing with this mask resulted in artificially elevated CO2 concentration measurements, which is most likely due to the dead space volume at the front of the mask. The mask also extends outward and into the supply gas stream, which may disrupt the washout effect of the suit supply gas. To mitigate these problems, a nasal cannula was investigated as a method for measuring inspired CO2 based on the assumptions that it is low profile and would not interfere with the designed suit gas flow path, and it has reduced dead space. This test series compared the performance of a nasal cannula to the oronasal mask in the Mark III space suit. Inspired CO2 levels were measured with subjects at rest and at metabolic workloads of 1000, 2000, and 3000 BTU/hr. Workloads were achieved by use of an arm ergometer or treadmill. Test points were conducted at air flow rates of 2, 4, and 6 actual cubic feet per minute, with a suit pressure of 4.3 psid. Results from this test series will evaluate the accuracy and repeatability across subjects of the nasal cannula collection method, which will provide rationale for using a nasal cannula as the new method for measuring inspired CO2 in a space suit. Proper characterization of sampling methods and of suit CO2 washout capability will better inform requirements definition and verification techniques for future CO2 washout limits in space suits

Meginnis, I↗

Multimode Acoustic Research

There is a need for high temperature containerless processing facilities that can efficiently position and manipulate molten samples in the reduced gravity environment of space. The goal of the research is to develop sophisticated high temperature manipulation capabilities such as selection of arbitrary axes rotation and rapid sample cooling. This program will investigate new classes of acoustic levitation in rectangular, cylindrical and spherical geometries. The program tasks include calculating theoretical expressions of the acoustic forces in these geometries for the excitation of up to three acoustic modes (multimodes). These calculations are used to: (1) determine those acoustic modes that produce stable levitation, (2) isolate the levitation and rotation capabilities to produce more than one axis of rotation, and (3) develop methods to translate samples down long tube cylindrical chambers. Experimental levitators will then be constructed to verify the stable levitation and rotation predictions of the models.

Barmatz, M.↗