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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 451 records · Page 25
All sourcesNASA NTRSDOE OSTI
DOE OSTI2019

US DOE In-Plant Trainings to Develop Expertise and Replicate Success

In-Plant Trainings (INPLTs) offered by the US Department of Energy Better Buildings, Better Plants (Better Plants) program are multi-day workshops performed by industry-recognized experts. INPLTs include both classroom- and field-based sessions that train attendees to identify energy conservation opportunities, quantify savings from these opportunities, and implement projects to realize the savings. These training workshops enhance the attendees’ understanding of working principles, knowledge of best practices, and capability to analyze the energy performance of industrial energy systems. INPLTs are competitively awarded and focus on a wide range of topics, including traditional industrial systems such as steam, process heating and motor-driven systems, and processes such as energy treasure hunts and municipal water and wastewater treatment. Partners that are awarded INPLTs can invite external stakeholders, such as employees of other manufacturers, utility representatives, and vendors. The Better Plants program had delivered 123 INPLTs as of spring 2019, trained more than 2,000 participants, and identified more than $37 million in energy cost savings. This paper provides an overview of INPLTs, some key statistics, the top identified energy savings opportunities, and success stories from INPLTs delivered from 2011 to 2018. Finally, the vision for future INPLTs is discussed based on participants’ feedback and the long-term and strategic goals of the Better Plants program.

Guo, Wei↗
DOE OSTI2020

Shape-preserving polymeric replication of biological matter

The present invention relates to a method of forming silica nanolayers on or within a biological sample, thereby capturing structural details that are present in the sample. The resultant silica composite or silica replica, in turn, can be employed as a template for a replica having those structural details captured in a polymer. Also provided herein are synthetic constructs including a polymeric replica, which can have any useful feature (e.g., oxygen-binding capacity).

36 MATERIALS SCIENCE↗
DOE OSTI2020

Optimizing software-directed instruction replication for GPU error detection

Software-only and software-hardware optimizations to reduce the overhead of intra-thread instruction duplication on a GPU or other instruction processor are disclosed. The optimizations trade off error containment for performance and include ISA extensions with limited hardware changes and area costs.

Hari, Siva↗
DOE OSTI2021

Shape-preserving polymeric replication of biological matter

The present invention relates to a method of forming silica nanolayers on or within a biological sample, thereby capturing structural details that are present in the sample. The resultant silica composite or silica replica, in turn, can be employed as a template for a replica having those structural details captured in a polymer. Also provided herein are synthetic constructs including a polymeric replica, which can have any useful feature (e.g., oxygen-binding capacity).

Kaehr, Bryan James↗
DOE OSTI2016

Construction of Nef-positive doxycycline-dependent HIV-1 variants using bicistronic expression elements

Conditionally replicating HIV-1 variants that can be switched on and off at will are attractive tools for HIV research. We previously developed a genetically modified HIV-1 variant that replicates exclusively when doxycycline (dox) is administered. The nef gene in this HIV-rtTA variant was replaced with the gene encoding the dox-dependent rtTA transcriptional activator. Because loss of Nef expression compromises virus replication in primary cells and precludes studies on Nef function, we tested different approaches to restore Nef production in HIV-rtTA. Strategies that involved translation via an EMCV or synthetic internal ribosome entry site (IRES) failed because these elements were incompatible with efficient virus replication. Fusion protein approaches with the FMDV 2A peptide and human ubiquitin were successful and resulted in genetically-stable Nef-expressing HIV-rtTA strains that replicate more efficiently in primary T-cells and human immune system (HIS) mice than Nef-deficient variants, thus confirming the positive effect of Nef on in vivo virus replication. - Highlights: • Different approaches to encode additional proteins in the HIV-1 genome were tested. • IRES translation elements are incompatible with efficient HIV-1 replication. • Ubiquitin and 2A fusion protein approaches allow efficient HIV-1 replication. • Doxycycline-controlled HIV-1 variants that encode all viral proteins were developed. • Nef stimulates HIV-rtTA replication in primary cells and human immune system mice.

60 APPLIED LIFE SCIENCES↗