General discussion of results and conclusions
Inverse relationship between retinal blood vessel caliber and oxygen level, creatinine level variations, and buildup in microorganisms during confinement of aviators in pure oxygen
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Inverse relationship between retinal blood vessel caliber and oxygen level, creatinine level variations, and buildup in microorganisms during confinement of aviators in pure oxygen
Effects of diet and pure oxygen environment on types and frequency of microorganisms in humans
Conversion of planetary environment by earth microorganisms to support human life
Life detection from phosphate and sulfur uptake and ATP production of microorganisms
Rocket and balloon-borne exposure study of terrestrial microorganism survival in space
Continuous synchronous culture of photosynthetic microorganism Chlorella cultivated in illuminated and dark stirred tanks
Planetary quarantine problem for Martian survival of microorganisms investigated, using systems analysis
Pyrolysis gas chromatography method for life detection and chemical identification of microorganisms
Growth of mixed cultures of microorganisms on mixed substrates
The Biomolecule Sequencer is a Commercial Off-The-Shelf device developed by Oxford Nanopore Technologies and implements a method of DNA sequencing unlike any other current sequencers. The device measures changes in electrical current through a nanopore depending on the sequence of the DNA strand that is passing through it. Since the technology is built on nanometer-scale ion pores, the hardware itself is exceptionally small (3 x 1 x 58 inches), lightweight (less than 120 grams with USB cable), and powered only by a USB connection. The sequencing device is permanent, while the flow cells, to which the samples are added, are periodically replaced. The goal of our upcoming technology demonstration on ISS is to provide evidence that DNA sequencing in space is possible, which holds the exciting potential to enable the identification of microorganisms, monitor changes in microbes and humans in response to spaceflight, and possibly aid in the detection of DNA-based life elsewhere in the universe.
Modern spacecraft design no longer involves only the Engineering Sciences. To get "the bugs" out of a system must now be taken literally to signify eradication of actual free-living microorganisms. A severe impact of a non-sterilized space vehicle could result in overt, reckless contamination, virtually eliminating a celestial body as a scientific base for the exobiological phases of space exploration. Recognizing the potentially damaging effects of septic launches, the National Aeronautics and Space Administration in December, 1960 authorized spacecraft sterilization and decontamination as National policy, thereby elevating the space effort onto a higher scientific plane. In the NASA directive, the basic reasons for sterilization were established, viz., "to preserve clues to the origin of life and of the universe, which may be hidden beneath the lunar strata or under the atmospheres of strange planets, to prevent inadvertent seeding of extraterrestrial surfaces by earth-life cultures, and to protect the earth from mutual contamination." In light of the foregoing, spacecraft sterilization should be regarded as the most significant single mission as well as design requirement of planetary space exploration --- especially if our objectives are primarily scientific.
Electromotive force production by microorganisms in a closed ecology for manned space flight
Physiological effects of space on animals, plants, microorganisms and biological substrates during suborbital and orbital flight
Problems of sampling for microorganisms in atmosphere
Wolf Trap device determining growth of microorganisms by measuring intensity of light scattered by organisms
Cytochemical studies of planetary microorganisms - deoxyribonuclease /dnase/ assay, fluorescein reactions, and enzyme activity
Approaches for analyzing possible life forms on other planets, noting detection of microorganisms or enzymes, radio contact and retracing life in laboratory
Coating for electronic components used in space applications has bacteriostatic qualities capable of hindering bacterial reproduction, both vegetative and sporulative viable microorganisms. It exhibits high electrical resistivity, a low outgassing rate, and is capable of restraining electronic components when subjected to mechanical vibrations.