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At least 451 records · Page 25

Population assignment of migratory Westslope Cutthroat Trout (WCT) in the Clark Fork–Pend Oreille River basin

Abstract Objective The Clark Fork–Pend Oreille River basin of northeastern Washington and the Idaho Panhandle historically supported a robust metapopulation of the Westslope Cutthroat Trout (WCT) Oncorhynchus lewisi, a western native salmonid of high cultural and economic value. The construction of impassible hydroelectric dams and smaller instream barriers has prevented the return of migratory WCT to spawning tributaries, leading to the fragmentation of this metapopulation over the past 100 years. One such impassible barrier is Albeni Falls Dam (AFD) near Newport, Washington, which was completed without fish passage capabilities in 1955. We sought to examine large-scale genetic patterns in the study area and determine the most likely spawning tributary of origin for migratory WCT captured below AFD. Methods We created a genetic baseline representative of populations within the Clark Fork–Pend Oreille River basin from upstream and downstream of the dam using 191 biallelic single-nucleotide polymorphism genetic markers. Our data set included 124 collections, which allowed for an examination of population structure and hatchery influence across the study area and provided a robust tool for population assignment. Population assignment tests were conducted using the program RUBIAS. Result Population assignment tests were successful for all pure WCT of unknown origin despite potential influence from hatchery lineages across the study area. Of 83 migratory WCT captured below AFD, approximately 80% were assigned to tributaries upstream of AFD with a posterior assignment probability of at least 90%. Only one fish was assigned to a tributary downstream of AFD. Conclusion Our results indicate that AFD disrupts the natural metapopulation dynamics of WCT populations in the basin. Passage for WCT at this barrier would reestablish metapopulation connectivity within the basin by allowing migratory individuals to make genetic contributions to populations upstream of the dam.

Wells, Craig D. (ORCID:0000000258513250)↗

PFLOTRAN-E4D: A parallel open source PFLOTRAN module for simulating time-lapse electrical resistivity data

Time-lapse electrical resistivity tomography (ERT) is finding increased application for remotely monitoring processes occurring in the near subsurface in three-dimensions (i.e. 4D monitoring). However, there are few codes capable of simulating the evolution of subsurface resistivity and corresponding tomographic measurements arising from a particular process, particularly in parallel and with an open source license. Herein we describe and demonstrate an electrical resistivity tomography module for the PFLOTRAN subsurface simulation code, named PFLOTRAN-E4D. The PFLOTRAN-E4D module operates in parallel using a dedicated set of compute cores in a master-slave configuration. At each time step, the master processes receives subsurface states from PFLOTRAN, converts those states to bulk electrical conductivity, and instructs the slave processes to simulate a tomographic data set. The resulting multi-physics simulation capability enables accurate feasibility studies for ERT imaging, the identification of the ERT signatures that are unique to a given process, and facilitates the joint inversion of ERT data with hydrogeological data for subsurface characterization. PFLOTRAN-E4D is demonstrated herein using a field study of stage-driven groundwater/river water interaction ERT monitoring along the Columbia River, Washington, USA. Results demonstrate the complex nature of changes subsurface electrical conductivity, in both the saturated and unsaturated zones, arising from water table changes and from river water intrusion into the aquifer. The results also demonstrate the sensitivity of surface based ERT measurements to those changes over time. PFLOTRAN-E4D is available with the PFLOTRAN development version with an open-source license at https://bitbucket.org/pflotran/pflotran-dev .

Environmental Molecular Sciences Laboratory↗

Injection and Monitoring at the Wallula Basalt Pilot Project

Continental flood basalts represent one of the largest geologic structures on earth but have received comparatively little attention for geologic storage of CO2. Flood basalt lava flows have flow tops that are porous, permeable, and have large potential capacity for storage of CO2. In appropriate geologic settings, interbedded sediment layers and dense low-permeability basalt rock flow interior sections may act as effective seals allowing time for mineralization reactions to occur. Previous laboratory experiments showed the relatively rapid chemical reaction of CO2-saturated pore water with basalts to form stable carbonate minerals. However, recent laboratory tests with water-saturated supercritical CO2 show that mineralization reactions occur in this phase as well, providing a second and potentially more important mineralization pathway than was previously understood. Field testing of these concepts is proceeding with drilling of the world’s first supercritical CO2 injection well in flood basalt being completed in May 2009 near the township of Wallula in Washington State and corresponding CO2 injection permit granted by the State of Washington in March 2011. Injection of a nominal 1000 MT of CO2 was completed in August 2013 and site monitoring is in progress. Well logging conducted immediately after injection termination confirmed the presence of CO2 predominantly within the upper flow top region, and showed no evidence of vertical CO2 migration outside the well casing. Shallow soil gas samples collected around the injection well show no evidence of leakage and fluid and gas samples collected from the injection zone show strongly elevated concentrations of Ca, Mg, Mn, and Fe and 13C/18O isotopic shifts that are consistent with basalt-water chemical reactions. If proven viable by this field test and others that are in progress or being planned, major flood basalts in the U.S., India, and perhaps Australia would provide significant additional CO2 storage capacity and additional geologic sequestration options in regions of these countries where conventional storage options are limited.

54 ENVIRONMENTAL SCIENCES↗

Reactivity of Redox Cycled Fe-bearing Subsurface Sediments Towards Hexavalent Chromium Reduction

Structural Fe(II) in clay minerals and natural sediments is known to reduce Cr(VI) to Cr(III), but the effect of redox-cycled Febearing natural sediments on Cr(VI) reduction kinetics is poorly understood. The objective of this study was to understand the kinetics and mechanisms of Cr(VI) reduction by Fe(II) in redox cycled natural sediment. Fe-bearing sediment was collected from the Ringold formation in the 300 area of Hanford, Washington, United States. Fe redox cycling of the sediment was accomplished via four cycles of bioreduction of structural Fe(III) in Hanford sediment and air oxidation of the resulting Fe(II). Bio-produced Fe(II) in Hanford sediment from each redox cycle was utilized to reduce Cr(VI) at three temperatures (10, 20 and 30° C). The initial rate of Cr(VI) reduction generally increased with each redox cycle, which was more pronounced at high temperatures. The amount of Fe(II) oxidized to the amount of Cr(VI) reduced was close to the expected stoichiometric ratio of 3. Aqueous concentrations of Si, Al, and Fe revealed some dissolution of the sediment after reaction with Cr(VI). Xray diffraction (XRD) and scanning electron microscopy (SEM) detected secondary mineral formation. Mössbauer data showed that the oxidation of Fe(II) was coupled with the reduction of Cr(VI) but no Feoxides/ oxyhydroxides formed. Transmission electron microscopy (TEM), electron energy loss spectroscopy (EELS), XANES, and EXAFS were performed for representative reduced Cr solids that formed from reduction of Cr(VI) by sediment-associated Fe(II) at 30oC. TEM revealed that the d-spacing of Cr-reacted montmorillonite at 30° C, a dominant Fe-bearing mineral in Hanford sediment, expanded from 10 Å to 13 Å. This layer expansion was likely due to intercalation of reduced Cr(III) into the interlayer space of the montmorillonite structure. EELS exhibited an L2 absorption peak at 586.0 eV and an L3 absorption peak at 577.0 eV, suggestive of Cr(III) in a hydroxide mineral phase. Similarly, XANES and EXAFS analyses confirmed Cr(VI) reduction to Cr(OH)3 at 30° C and indicated an edge-sharing coordination of the Cr(III) octahedra to 2-3 other Cr or metal ions. This study has important implications for understanding the reactivity of clay-rich sediment towards Cr(VI) reduction at contaminated sites and the stability of the reduced Cr(III).

Bishop, Michael E.↗

Line shape parameters of air-broadened water vapor transitions in the nu(1) and nu(3) spectral region

A Bruker IFS-120HR Fourier transform spectrometer located at the Pacific Northwest Laboratory (PNNL) in Richland, Washington was used to record a series of spectra of pure H2O and air-broadened H2O in the regions of the ¦1 and ¦3 bands (3450-4000 cm-1) at different pressures, mperatures and volume mixing ratios of H2O in air. 18 high-resolution, high signal- to-noise (S/N) ratio absorption spectra were recorded at T = 268, 296 and 353 K using two temperature-controlled absorption cells with path lengths of 9.906(1) and 19.95(1) cm. The resolution of the spectra recorded with the 9.906 cm and 19.95 cm absorption cells was 0.006 and 0.008 cm-1, respectively. A multispectrum nonlinear least squares fitting technique was employed to fit all the eighteen spectra simultaneously to retrieve 313 accurate line positions, 315 intensities, 229 Lorentz air-broadened half- width and 213 air-shift coefficients and their temperature dependences (136 for air-broadened width and 128 for air-shift coefficients, respectively). Room temperature self-broadened half-width coefficients for 209 transitions and self-shift coefficients for 106 transitions were also measured. Line mixing coefficients were experimentally determined for isolated sets of 10 transition pairs for H2O-air and 8 transition pairs for H2O-H2O using the off-diagonal relaxation matrix element formalism, and 85 quadratic speed dependence parameters were measured. Modified Complex Robert-Bonamy (MCRB) calculations of self-, and air-broadened (from N2- and O2-broadening) half-width and air-shift coefficients, and temperature dependence exponents of air-broadened half-width coefficients are made. The measurements and calculations are compared with each other and with similar parameters reported in the literature.

H2O, spectral line shapes, air-broadening, tempera↗

Radiation damage and annealing in plutonium tetrafluoride

Plutonium tetrafluoride that was separated prior to 1966 at the Hanford Site in Washington State was analyzed at the Pacific Northwest National Laboratory (PNNL) in 2015 and 2016. The plutonium tetrafluoride, as received, was an off-normal color and considering the age of the plutonium, there were questions about the condition of the material. These questions had to be answered in order to determine the suitability of the material for future use or long-term storage. Therefore, Thermogravimetric/Differential Thermal Analysis and X-ray Diffraction evaluations were conducted to determine the plutonium’s crystal structure, oxide content, and moisture content; these analyses reported that the plutonium was predominately amorphous and tetrafluoride, with an oxide content near ten percent. Freshly fluorinated plutonium tetrafluoride is known to be monoclinic. During the initial Thermogravimetric/Differential Thermal analyses, it was discovered that an exothermic event occurred within the material near 414°C. X-ray Diffraction analyses were conducted on the annealed tetrafluoride. The X-ray Diffraction analyses indicated that some degree of recrystallization occurred in conjunction with the 414°C event. The following commentary describes the series of Thermogravimetric/Differential Thermal and X-ray Diffraction analyses that were conducted as part of this investigation at PNNL, in collaboration with the University of Utah Nuclear Engineering Program.

McCoy, Kaylyn↗

Shared micromobility as a first- and last-mile transit solution? Spatiotemporal insights from a novel dataset

The first- and last-mile (FM/LM) problem is a major deterrent to public transit use. With the rise of shared micromobility options such as shared e-scooters in recent years, there is a growing interest in understanding their potential to serve as a last-mile transit solution. However, empirical data regarding the integrated use of shared micromobility and public transit have been limited so far. As a result, much is unknown regarding the spatiotemporal patterns and characteristics of shared micromobility trips serving as an FM/LM connection to transit. Here, this paper addresses these knowledge gaps by leveraging a novel dataset (i.e., the Spin post-ride survey dataset) that records thousands of transit-connecting shared e-scooter trips in Washington DC. Specifically, we used the dataset to reveal the spatiotemporal patterns of transit-connecting shared e-scooter trips in Washington DC, resulting in some major policy insights regarding the integral use of shared e-scooters and public transit. We further leveraged the dataset to validate if and to what extent a commonly applied buffer-zone approach can infer FM/LM micromobility trips accurately. Statistical tests showed that the actual FM/LM Spin e-scooter trips differ from inferred FM/LM Spin e-scooter trips in both spatial and temporal dimensions. This indicates that the common practice of inferring FM/LM micromobility trips with a buffer-zone approach can lead to inaccurate estimates of transit-connecting micromobility trips.

29 ENERGY PLANNING, POLICY, AND ECONOMY↗

Characterizing spatiotemporal dynamics in phenology of urban ecosystems based on Landsat data

Seasonal phenology of vegetation plays an important role in global carbon cycle and ecosystem productivity. In urban environments, vegetation phenology is also important because of its influence on public health (e.g., allergies), and energy demand (e.g. cooling effects). In this study, we studied the potential use of remotely sensed observations (i.e. Landsat data) to derive some phenology indicators for vegetation embedded within the urban core domains in four distinctly different U.S. regions (Washington, D.C., King County in Washington, Polk County in Iowa, and Baltimore City and County in Maryland) during the past three decades. We used all available Landsat observations (circa 3000 scenes) from 1982 to 2015 and a self-adjusting double logistic model to detect and quantify the annual change of vegetation phenophases, i.e. indicators of seasonal changes in vegetation. The proposed model can capture and quantify not only phenophases of dense vegetation in rural areas, but also those of mixed vegetation in urban core domains. The derived phenology indicators show a good agreement with similar indicators derived from the Moderate Resolution Imaging Spectroradiometer (MODIS) and in situ observations, suggesting that the phenology dynamic depicted by the proposed model is reliable. The vegetation phenology and its seasonal and interannual dynamics demonstrate a distinct spatial pattern in urban domains with an earlier (9–14 days) start-of season (SOS) and a later (13–20 days) end-of season (EOS), resulting in an extended (5–30 days) growing season length (GSL) when compared to the surrounding suburban and rural areas in the four study regions. There is a general long-term trend of decreasing SOS (-0.30 day per year), and increasing EOS and GSL (0.50 and 0.90 day per year, respectively) over past three decades for these study regions. The magnitude of these trends varies among the four urban systems due to their diverse local climate conditions, vegetation types, and different urban-rural settings. The Landsat derived phenology information for urban domains provides more details when compared to the coarse-resolution datasets such as MODIS, thus improves our understanding of human-natural systems interactions (or feedbacks) in urban domains. Such information is very valuable for urban planning in light of rapid urbanization and expansion of major metropolitans at the national and global levels.

60 APPLIED LIFE SCIENCES↗

Technetium and iodine aqueous species immobilization and transformations in the presence of strong reductants and calcite-forming solutions: Remedial action implications

At the Hanford Site in southeastern Washington State, radionuclide (Tc-99/I-129) laden liquid wastes have been discharged to ground, resulting in vadose zone contamination, which provides a continuous source of these contaminants to groundwater. The presence of multiple contaminants increases the complexity of finding viable remediation technologies to sequester vadose zone contaminants in situ and protect groundwater. Although previous studies have shown the efficiency of zero valent iron (ZVI) and sulfur modified iron (SMI) in reducing mobile Tc(VII) to immobile Tc(IV) and iodate incorporation into calcite, the coupled effects from simultaneously using these remedial technologies have not been previously studied. In this first-of-a-kind laboratory study, we used two efficient reductants (i.e., ZVI and SMI) and calcite-forming solutions to simultaneously remove aqueous Tc(VII) and iodate via reduction and incorporation, respectively. The results confirmed that Tc(VII) was rapidly removed from the aqueous phase via reduction to Tc(IV). ZVI removed Tc(VII) faster than SMI, although both had removed the same amount by the end of the experiments. Most of the aqueous iodate was rapidly transformed to iodide, and therefore was not incorporated into calcite, but instead remained in the aqueous phase. The iodate reduction to iodide was much faster than iodate incorporation into calcite, suggesting that this remedial pathway is not efficient in removing aqueous iodate when strong reductants are present. Other experiments suggested that iodate removal via calcite precipitation should occur first and then reductants should be added for Tc(VII) removal. Although ZVI can negatively impact microbial populations and thereby inhibit natural attenuation mechanisms, only changes in the makeup of the microbial community were observed. However, these changes in the microbial community may have an impact on remediation efforts in the long term that could not be seen in a short-term study. The results underscore the importance of identifying interactions between natural attenuation pathways and remediation technologies that only target individual contaminants.

Keywords: Tc removal, groundwater remediation, nuc↗

Phosphate-Induced Immobilization of Uranium in Hanford Sediments

Phosphate can be added to subsurface environments to immobilize U(VI) contamination. The efficacy of immobilization depends on the site-specific groundwater chemistry and aquifer sediment properties. Batch and column experiments were performed with sediments from the Hanford 300 Area in Washington State and artificial groundwater prepared to emulate the conditions at the site. Batch experiments revealed enhanced U(VI) sorption with increasing phosphate addition. X-ray absorption spectroscopy measurements of samples from the batch experiments found that U(VI) was predominantly adsorbed at conditions relevant to the column experiments and most field sites (low U(VI) loadings, <25 μM), and U(VI) phosphate precipitation occurred only at high initial U(VI) (>25μM) and phosphate loadings. While batch experiments showed the transition of U(VI) uptake from adsorption to precipitation, the column study was more directly relevant to the subsurface environment because of the high solid:water ratio in the column and the advective flow of water. In column experiments, nearly six times more U(VI) was retained in sediments when phosphate-containing groundwater was introduced to U(VI)-loaded sediments than when the groundwater did not contain phosphate. This enhanced retention persisted for at least one month after cessation of phosphate addition to the influent fluid. Sequential extractions and laser-induced fluorescence spectroscopy of sediments from the columns suggested that the retained U(VI) was primarily in adsorbed forms. These results indicate that in situ remediation of groundwater by phosphate addition provides lasting benefit beyond the treatment period via enhanced U(VI) adsorption to sediments.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

The Uranium from Seawater Program at the Pacific Northwest National Laboratory: Overview of Marine Testing, Adsorbent Characterization, Adsorbent Durability, Adsorbent Toxicity, and Deployment Studies

The Pacific Northwest National Laboratory’s (PNNL) Marine Science Laboratory (MSL) located along the coast of Washington State is evaluating the performance of uranium adsorption materials being developed for seawater extraction under realistic marine conditions with natural seawater. Two types of exposure systems were employed in this program: flow-through columns for testing of fixed beds of individual fibers and pellets and a recirculating water flume for testing of braided adsorbent material. Testing consists of measurements of the adsorption of uranium and other elements from seawater as a function of time, typically 42 to 56 day exposures, to determine the adsorbent capacity and adsorption rate (kinetics). Analysis of uranium and other trace elements collected by the adsorbents was conducted following strong acid digestion of the adsorbent with 50% aqua regia using either Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES) or Inductively Coupled Plasma Mass Spectrometer (ICP-MS). The ORNL 38H adsorbent had a 56 day adsorption capacity of 3.30 ± 0.68 g U/ kg adsorbent (normalized to a salinity of 35 psu), a saturation adsorption capacity of 4.89 ± 0.83 g U/kg of adsorbent material (normalized to a salinity of 35 psu) and a half-saturation time of 28 ± 10 days. The AF1 adsorbent material had a 56 day adsorption capacity of 3.9 ± 0.2 g U/kg adsorbent material (normalized to a salinity of 35 psu), a saturation capacity of 5.4 ± 0.2 g U/kg adsorbent material (normalized to a salinity of 35 psu) and a half saturation time of 23 ± 2 days. The ORNL amidoxime-based adsorbent materials are not specific for uranium, but also adsorb other elements from seawater. The major doubly charged cations in seawater (Ca and Mg) account for a majority of the cations adsorbed (61% by mass and 74% by molar percent). For the ORNL AF1 adsorbent material, U is the 4th most abundant element adsorbed by mass and 7th most abundant by molar percentage

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Delineating Facies Spatial Distribution by Integrating Ensemble Data Assimilation and Indicator Geostatistics with Level-Set Transformation

The facies-based approach has been widely adopted to delineate an aquifer into distinct geological units with unique distributions of hydraulic, physical, and/or chemical properties. The recent development in ensemble-based data assimilation methods allows both the direct and indirect data to be used to improve facies delineation. A major difficulty in those applications is to honor the spatial continuity and avoid overfitting after data assimilation. We introduce a new facies delineation framework to integrate ensemble data assimilation with traditional transition probability-based geostatistics. A level-set concept is used to parametrize discrete facies indicators and for updating facies shape. During the iterative data assimilation process, we impose spatial continuity by conditioning facies field generation on points selected adaptively based on their sensitivity to observation data. This reconditioning step is a key step to maintain spatial continuity and overcome over-fitting problems in inversion. We selected two examples to evaluate the performance of the new framework in estimating facies-based permeability field. The first example is a two-dimensional synthetic system with transient head data induced by pumping tests used for delineating two facies. The second example is a three-dimensional case with three facies, conceptualized from a field tracer experiment within the Columbia River corridor in Washington State, USA. Both examples demonstrate that the new method can adequately capture the spatial pattern of hydrofacies with reconditioning, which leads to the improved prediction of system behaviors.

Song, Xuehang↗

CreelCat, a Catalog of United States Inland Creel and Angler Survey Data

The United States Inland Creel and Angler Survey Catalog (CreelCat) contains a national compilation of angler and creel survey data collected by natural resource management agencies across the United States (including Washington, D.C. and Puerto Rico). These surveys are used to help inform the management of recreational fisheries, by collecting information about anglers including what they are catching and harvesting, the amount of effort they expend, their angling preferences, and demographic information. As of May 1, 2023, CreelCat houses over 14,729 surveys from 33 states, Puerto Rico, and Washington, D.C., comprising 235 data fields across 8 tables. These tables contain 235,015 records of fish catch and harvest metrics, 27,250 angler preference metrics, 14,729 records of survey characteristics, 13,576 records of effort metrics, and 409 records of angler demographics. Though individual creel surveys are often deployed to meet local science and management objectives, creel data aggregated across jurisdictions has the potential to address larger scale research and management needs.

59 BASIC BIOLOGICAL SCIENCES↗

Evaluating the WFIP2 updates to the HRRR model using scanning Doppler lidar measurements in the complex terrain of the Columbia River Basin

The wind-energy (WE) industry relies on numerical weather prediction (NWP) forecast models as foundational or base models for many purposes, including wind-resource assessment and wind-power forecasting. During the Second Wind Forecast Improvement Project (WFIP2) in the Columbia River Basin of Oregon and Washington, a significant effort was made to improve NWP forecasts through focused model development, to include experimental refinements to the High Resolution Rapid Refresh (HRRR) model physics and horizontal grid spacing. In this study, the performance of an experimental version of HRRR that includes these refinements is tested against a control version, which corresponds to that of the operational HRRR run by National Oceanic and Atmospheric Administration/National Centers for Environmental Protection at the outset of WFIP2. Furthermore, the effects of horizontal grid resolution were also tested by comparing wind forecasts from the HRRR (with 3-km grid spacing) with those from a finer-resolution HRRR nest with 750-m grid spacing. Model forecasts are validated against accurate wind-profile measurements by three scanning, pulsed Doppler lidars at sites separated by a total distance of 71 km. Model skill and improvements in model skill, attributable to physics refinements and improved horizontal grid resolution, varied by season, by site, and during periods of atmospheric phenomena relevant to WE. In general, model errors were the largest below 150 m above ground level (AGL). Experimental HRRR refinements tended to reduce the mean absolute error (MAE) and other error metrics for many conditions, but degradation in skill (increased MAE) was noted below 150 m AGL at the two lowest-elevation sites at night. Finer resolution was found to produce the most significant reductions in the error metrics.

17 WIND ENERGY↗

Estimating Cohort Survival through Tributaries for Salmonid Populations with Variable Ages at Migration

Abstract Survival of juvenile anadromous Pacific salmonids from their earliest age of seaward movement (“out-migration”) through the tributaries that connect their rearing grounds to larger-order rivers (“cohort survival”) is an important yet often unmonitored factor in the complex life history of these species. Populations with variable age at out-migration (e.g., steelhead Oncorhynchus mykiss) or multiple juvenile rearing strategies (e.g., Chinook Salmon O. tshawytscha) raise particular challenges in survival monitoring. A multiple-state release–recapture model is presented to estimate cohort survival for salmonid species with variable age at out-migration and demonstrated in two case studies. Annual releases of fish tagged with passive integrated transponder (PIT) tags from the same brood year (cohort) and their detections by instream PIT-tag detection arrays in tributaries and at hydroelectric dams are used to estimate survival through tributaries and the age composition of juvenile migrants reaching downstream sites. The 2010 cohort of Chinook Salmon from the Chiwawa River in Washington State had estimated survival of 0.59 to the middle Wenatchee River array in Tumwater Dam Reservoir (river distance of 30 km) and 0.12 to McNary Dam (364 km) on the Columbia River. The age composition shifted from 60.1% subyearlings at the Wenatchee River array to 100% yearlings at McNary Dam, reflecting the use of the Wenatchee River as a nonnatal rearing area, whereas McNary Dam is in the migratory corridor. The 2010 cohort of steelhead from the Twisp River in Washington State had estimated survival from the yearling stage of 0.30 to Rocky Reach Dam (146 km from the Twisp River mouth) on the Columbia River and 0.17 to McNary Dam (439 km). The low survival to the Columbia River resulted from both mortality and adoption of a resident life history and reflects the diverse population structure in the Twisp River. Received February 4, 2015; accepted June 11, 2015

Buchanan, Rebecca A.↗

Baryon number violation: from nuclear matrix elements to BSM physics

Processes that violate baryon number, most notably proton decay and $n\bar{n}$ transitions, are promising probes of physics beyond the Standard Model (BSM) needed to understand the lack of antimatter in the Universe. To interpret current and forthcoming experimental limits, theory input from nuclear matrix elements to UV complete models enters. Thus, an interplay of experiment, effective field theory, lattice QCD, and BSM model building is required to develop strategies to accurately extract information from current and future data and maximize the impact and sensitivity of next-generation experiments. Here, we briefly summarize the main results and discussions from the workshop ‘INT-25-91W: Baryon Number Violation: From Nuclear Matrix Elements to BSM Physics,’ held at the Institute for Nuclear Theory, University of Washington, Seattle, WA, 13–17 January 2025.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

Legacy Effects of Cropping System and Precipitation Influence the Core Camelina sativa Microbiome

Camelina ( Camelina sativa L.) is a potential biofuel crop and beneficial rotation crop in dryland cropping systems. Little is known about camelina microbiota or the legacy effect of soil origin/cropping system zones on camelina-associated microbiome assembly. To explore camelina-microbe associations, we grew camelina in the greenhouse using soil transplanted from 33 locations in the dryland wheat production area of eastern Washington. Bacterial, archaeal, and fungal communities from bulk soil, rhizosphere, and endosphere were characterized with 16S rRNA and internal transcribed spacer amplicon sequencing and were analyzed alongside site-specific climatic and edaphic data. We found that soil from the highest precipitation zone had higher alpha diversity than soil from the driest zone, but this effect was not seen in the greenhouse rhizosphere or endosphere. Plant compartment, cropping system zone, and soil origin all significantly influenced microbial composition, with soil pH and organic matter, as well as precipitation at origin, as major predictors. Analysis of abundance–occupancy distributions showed that the Actinobacteriota Aeromicrobium and Marmoricola and the fungus Pseudogymnoascus in the rhizosphere were plant-selected, while the endosphere was characterized by a number of Actinobacteriota, Rhizobium, and Clostridium. Sphingomonas amplicon sequence variants were also consistently enriched in the rhizosphere, suggesting that they are present in soils collected throughout eastern Washington and may represent good candidate biostimulants. Several lignin decomposing fungi had site-specific rhizospheric distributions, suggesting that they may be dispersal-limited or result from the legacy effect of long-term wheat cropping. Overall, this study contributes to our understanding of microbiome assembly in and on camelina roots while also highlighting the potential impact of cropping history on soil- and plant-associated microbiomes. [Formula: see text] The author(s) have dedicated the work to the public domain under the Creative Commons CC0 “No Rights Reserved” license by waiving all of his or her rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law, 2025.

Barnes, Elle M↗