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DOE OSTI2019

Testing dark matter and modifications to gravity using local Milky Way observables

Galactic rotation curves are often considered the first robust evidence for the existence of dark matter. Yet, even in the presence of a dark matter halo, other galactic-scale observations, such as the Baryonic Tully-Fisher Relation and the Radial Acceleration Relation, remain challenging to explain. This has motivated long-distance, infrared modifications to gravity as an alternative to the dark matter hypothesis as well as various DM theories with similar phenomenology. In general, the standard lore has been that any model that reduces to the phenomenology of MOdified Newtonian Dynamics (MOND) on galactic scales explains essentially all galaxy-scale observables. We present a framework to test precisely this statement using local Milky Way observables, including the vertical acceleration field, the rotation curve, the baryonic surface density, and the stellar disk profile. We focus on models that predict scalar amplifications of gravity, i.e., models that increase the magnitude but do not change the direction of the gravitational acceleration. We find that models of this type are disfavored relative to a simple dark matter halo model because the Milky Way data necessitates a substantial amplification of the radial acceleration with little amplification of the vertical acceleration. We conclude that models which result in a MOND-like force struggle to simultaneously explain both the rotational velocity and vertical motion of nearby stars in the Milky Way.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

DOE OSTI2020

Hotspot parameter scaling with velocity and yield for high-adiabat layered implosions at the National Ignition Facility

Here, in this paper, we present a study on hotspot parameters in indirect-drive, inertially confined fusion implosions as they proceed through the self-heating regime. The implosions with increasing nuclear yield reach the burning-plasma regime, hotspot ignition, and finally propagating burn and ignition. These implosions span a wide range of alpha heating from a yield amplification of 1.7–2.5. We show that the hotspot parameters are explicitly dependent on both yield and velocity and that by fitting to both of these quantities the hotspot parameters can be fit with a single power law in velocity. The yield scaling also enables the hotspot parameters extrapolation to higher yields. This is important as various degradation mechanisms can occur on a given implosion at fixed implosion velocity which can have a large impact on both yield and the hotspot parameters. The yield scaling also enables the experimental dependence of the hotspot parameters on yield amplification to be determined. The implosions reported have resulted in the highest yield (1.73×10 16 ±2.6%), yield amplification, pressure, and implosion velocity yet reported at the National Ignition Facility.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

DOE OSTI2021

Bandwidth extension and conversion efficiency improvements beyond phase matching limitations using cavity-enhanced OPCPA

The conversion efficiency and phase matching bandwidth of ultrafast optical parametric amplification (OPA) are constrained by the dispersion and nonlinear coefficient of the employed crystal as well as pulse shaping effects. In our work we show that an enhancement cavity resonant with the pump seeded at the full repetition rate of the pump laser can automatically reshape the small-signal gain in optical parametric chirped-pulse amplification (OPCPA) to achieve close-to-optimal operation. This new method termed cavity-enhanced OPCPA or C-OPCPA significantly increases both the gain bandwidth and the conversion efficiency, in addition to boosting gain for high-repetition-rate amplification. The goal in C-OPCPA is to arrive at a condition of impedance matching at all temporal coordinates, such that, in the absence of linear losses, all the incident pump power is dissipated in the nonlinear loss element, i.e., converted to signal and idler. The use of a low finesse enhancement cavity resonant with a low average power (<1W) and a high repetition rate (78MHz) pump source is shown to achieve more than 50% conversion efficiency into signal and idler from the coupled pump in an optical parametric process, whereas an equivalent amount of pump power in a single-pass configuration leads to negligible conversion. Additionally, the gain bandwidth is extended by a factor of 3-4 beyond the phase-matching limit. Our empirical observations are corroborated by a numerical analysis of depletion optimizing the single-pass case, which assesses the underlying impedance matching that is responsible for the observed performance improvements.

47 OTHER INSTRUMENTATION↗

DOE OSTI2022

Effect of the pump beam profile and wavefront on the amplified signal wavefront in optical parametric amplifiers

We present a theoretical and experimental analysis of the signal phase introduced by the pump-beam wavefront and spatial profile during optical parametric amplification (OPA) process. The theory predicts the appearance of an additional wavefront in the amplified signal beam that is proportional to the spatial derivative of the pump-beam wavefront. The effect of the pump-beam profile on the signal-beam wavefront is also investigated. Our experiments tested these theoretical predictions by comparing the wavefront of the signal beam before and after amplification in a multi-joule broadband OPA. The measured signal wavefront was shown to have the expected dependence on the pump-beam profile and wavefront. These results can be considered when designing petawatt-scale ultrabroadband optical parametric chirped-pulse–amplification systems.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

DOE OSTI2019

Predicting Ionizing Radiation Exposure (LDRD Final Report)

Rapid, low-cost, easy-to-use devices are needed for screening large populations potentially exposed to ionizing radiation following accidental or intentional release of radioactive materials. Measurements of radiation responsive messenger RNA (mRNA) biomarkers using fieldable devices could provide such a tool. mRNA expression level changes may be useful for discriminating between exposed individuals and the worried well and estimating the radiation dose received even days after exposure. Loop mediated isothermal amplification, or LAMP, is an established nucleic acid amplification technique that provides key advantages for use in low cost, portable instruments. We proposed to use LAMP to determine if the expression level of one or a small number of radiation responsive biomarkers can be used to discriminate unexposed cells from ionizing radiation (IR)-exposed cells. If successful, this methodology could potentially be transitioned to portable detection technology for use in population screening after large-scale events. Our approach involved designing quantitative, real-time, reverse transcriptase loop-mediated isothermal amplification (RTLAMP) assays for a panel of published IR-responsive mRNA biomarkers and control genes. This assay panel was then tested on mRNA isolated from white blood cells, lymphocyte cell lines and samples from internally dosed individuals. We used standard curves to quantify mRNA copy number along with the comparative Ct method to measure relative mRNA expression changes. We were able to generate quantitative and comparative gene expression changes for many of the samples evaluated. Although promising in its potential to measure gene expression, the technique suffered when attempting to amplify target genes with very low background RNA expression levels, in particular, FDXR in controls samples. Future work should focus on optimizing the RT-LAMP reaction for low RNA expression level targets allowing for the generation of validated assay panels.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2026

MOD-Amp System Design Spring: Spring 2026 – Georgetown University, SYSM–5620

High-energy lasers (HELs) play an important role in both national defense and scientific research. In defense applications, HELs are used for target detection, tracking, and engagement. In research environments, they support studies of extreme physical conditions relevant to fusion energy and plasma science. These systems depend on the amplification of light through stimulated emission of radiation, allowing optical energy to be increased to the levels required for operation. This amplification occurs when light passes through an energized gain medium that receives energy from an external optical or electrical source. To achieve the desired output, laser systems often use multiple amplification stages, including high-gain preamplifiers and lower-gain power or booster amplifiers. At Lawrence Livermore National Laboratory (LLNL) and other national laboratories, many large-aperture laser amplifier systems are aging and rely on system-specific hardware, obsolete technologies, and incomplete documentation. These legacy systems create challenges for maintenance, supportability, and long-term operation. Their lack of standardization also increases the difficulty of sustaining reliable performance over time. As this infrastructure continues to age, the likelihood of unplanned downtime grows, which can negatively affect both national security missions and scientific research programs that depend on dependable HEL capabilities. The purpose of this document is to demonstrate the application of systems engineering fundamentals and design thinking through the development of a laser amplifier case study. The proposed system concept is intended as an academic exercise and not as a finalized engineering design. As a result, the development presented in this document is incomplete and may contain technical assumptions or errors that would require further investigation before any real-world implementation.

42 ENGINEERING↗

DOE OSTI2026

Energy Partition in Collisionless Counterstreaming Plasmas

Fast, counterstreaming plasma outflows drive magnetic field amplification, plasma heating, and particle acceleration in numerous astrophysical environments, from supernova remnant shocks to active galactic nuclei jets. Understanding how, in the absence of Coulomb collisions, energy is redistributed between the different plasma species remains a fundamental open question. We use three-dimensional, fully kinetic simulations to investigate energy partition in weakly magnetized counterpropagating plasmas. Our results reveal a complex interplay between different processes, where at early times the Weibel instability drives a first stage of magnetic field amplification and at late times the kinking of current filaments drives a second amplification stage via a dynamo-type mechanism. Electrons are heated primarily during the latter phase through magnetic pumping. By the time the flows thermalize, we observe that the final temperature ratio T e /T i and energy partition depend on the ion-to-electron mass ratio. For electron–proton flows, the electron thermal energy only reaches up to a few percent of the initial ion kinetic energy.

Marret, Alexis [SLAC National Accelerator Laborato↗

DOE OSTI2018

Optimizing de novo genome assembly from PCR-amplified metagenomes

Background. Metagenomics has transformed our understanding of microbial diversity across ecosystems, with recent advances enabling de novo assembly of genomes from metagenomes. These metagenome-assembled genomes are critical to provide ecological, evolutionary, and metabolic context for all the microbes and viruses yet to be cultivated. Metagenomes can now be generated from nanogram to subnanogram amounts of DNA. However, these libraries require several rounds of PCR amplification before sequencing, and recent data suggest these typically yield smaller and more fragmented assemblies than regular metagenomes. Methods. Here we evaluate de novo assembly methods of 169 PCR-amplified metagenomes, including 25 for which an unamplified counterpart is available, to optimize specific assembly approaches for PCR-amplified libraries. We first evaluated coverage bias by mapping reads from PCR-amplified metagenomes onto reference contigs obtained from unamplified metagenomes of the same samples. Then, we compared different assembly pipelines in terms of assembly size (number of bp in contigs ≥ 10kb) and error rates to evaluate which are the best suited for PCR-amplified metagenomes. Results. Read mapping analyses revealed that the depth of coverage within individual genomes is significantly more uneven in PCR-amplified datasets versus unamplified metagenomes, with regions of high depth of coverage enriched in short inserts. This enrichment scales with the number of PCR cycles performed, and is presumably due to preferential amplification of short inserts. Standard assembly pipelines are confounded by this type of coverage unevenness, so we evaluated other assembly options to mitigate these issues. We found that a pipeline combining read deduplication and an assembly algorithm originally designed to recover genomes from libraries generated after whole genome amplification (single-cell SPAdes) frequently improved assembly of contigs ≥ 10kb by 10 to 100-fold for low input metagenomes. Conclusions. PCR-amplified metagenomes have enabled scientists to explore communities traditionally challenging to describe, including some with extremely low biomass or from which DNA is particularly difficult to extract. Here we show that a modified assembly pipeline can lead to an improved de novo genome assembly from PCR-amplified datasets, and enables a better genome recovery from low input metagenomes.

Roux, Simon↗

DOE OSTI2018

Optimizing de novo genome assembly from PCR-amplified metagenomes

Background: Metagenomics has transformed our understanding of microbial diversity across ecosystems, with recent advances enabling de novo assembly of genomes from metagenomes. These metagenome-assembled genomes are critical to provide ecological, evolutionary, and metabolic context for all the microbes and viruses yet to be cultivated. Metagenomes can now be generated from nanogram to subnanogram amounts of DNA. However, these libraries require several rounds of PCR amplification before sequencing, and recent data suggest these typically yield smaller and more fragmented assemblies than regular metagenomes. Methods: Here we evaluate de novo assembly methods of 169 PCR-amplified metagenomes, including 25 for which an unamplified counterpart is available, to optimize specific assembly approaches for PCR-amplified libraries. We first evaluated coverage bias by mapping reads from PCR-amplified metagenomes onto reference contigs obtained from unamplified metagenomes of the same samples. Then, we compared different assembly pipelines in terms of assembly size (number of bp in contigs ≥ 10kb) and error rates to evaluate which are the best suited for PCR-amplified metagenomes. Results: . Read mapping analyses revealed that the depth of coverage within individual genomes is significantly more uneven in PCR-amplified datasets versus unamplified metagenomes, with regions of high depth of coverage enriched in short inserts. This enrichment scales with the number of PCR cycles performed, and is presumably due to preferential amplification of short inserts. Standard assembly pipelines are confounded by this type of coverage unevenness, so we evaluated other assembly options to mitigate these issues. We found that a pipeline combining read deduplication and an assembly algorithm originally designed to recover genomes from libraries generated after whole genome amplification (single-cell SPAdes) frequently improved assembly of contigs ≥ 10kb by 10 to 100-fold for low input metagenomes. Conclusions: PCR-amplified metagenomes have enabled scientists to explore communities traditionally challenging to describe, including some with extremely low biomass or from which DNA is particularly difficult to extract. Here we show that a modified assembly pipeline can lead to an improved de novo genome assembly from PCR-amplified datasets, and enables a better genome recovery from low input metagenomes.

Roux, Simon↗

DOE OSTI2019

Optimizing de novo genome assembly from PCR-amplified metagenomes

Background. Metagenomics has transformed our understanding of microbial diversity across ecosystems, with recent advances enabling de novo assembly of genomes from metagenomes. These metagenome-assembled genomes are critical to provide ecological, evolutionary, and metabolic context for all the microbes and viruses yet to be cultivated. Metagenomes can now be generated from nanogram to subnanogram amounts of DNA. However, these libraries require several rounds of PCR amplification before sequencing, and recent data suggest these typically yield smaller and more fragmented assemblies than regular metagenomes. Methods. Here we evaluate de novo assembly methods of 169 PCR-amplified metagenomes, including 25 for which an unamplified counterpart is available, to optimize specific assembly approaches for PCR-amplified libraries. We first evaluated coverage bias by mapping reads from PCR-amplified metagenomes onto reference contigs obtained from unamplified metagenomes of the same samples. Then, we compared different assembly pipelines in terms of assembly size (number of bp in contigs ≥ 10 kb) and error rates to evaluate which are the best suited for PCR-amplified metagenomes. Results. Read mapping analyses revealed that the depth of coverage within individual genomes is significantly more uneven in PCR-amplified datasets versus unamplified metagenomes, with regions of high depth of coverage enriched in short inserts. This enrichment scales with the number of PCR cycles performed, and is presumably due to preferential amplification of short inserts. Standard assembly pipelines are confounded by this type of coverage unevenness, so we evaluated other assembly options to mitigate these issues. We found that a pipeline combining read deduplication and an assembly algorithm originally designed to recover genomes from libraries generated after whole genome amplification (single-cell SPAdes) frequently improved assembly of contigs ≥10 kb by 10 to 100-fold for low input metagenomes. Conclusions. PCR-amplified metagenomes have enabled scientists to explore communities traditionally challenging to describe, including some with extremely low biomass or from which DNA is particularly difficult to extract. Here we show that a modified assembly pipeline can lead to an improved de novo genome assembly from PCR-amplified datasets, and enables a better genome recovery from low input metagenomes.

59 BASIC BIOLOGICAL SCIENCES↗

NASA NTRS1980

Coherent substructure of turbulence near the stagnation zone of a bluff body

The evolution of freestream turbulence in crossflow about a circular cylinder was studied in order to identify the existence of a coherent substructure which is the outcome of the amplification of freesteam turbulence by the stretching mechanism in diverging flow about a bluff body. Visualization of the flow events revealed the selective stretching of cross-vortex tubes and the emergence of an organized turbulent flow pattern near the cylinder stagnation zone. Significant amplification of the total turbulent energy of the streamwise fluctuating velocity was consistently monitored. Realization of selective amplification at scales larger than the neutral scale of the stagnation flow was indicated by the variation of the discrete streamwise turbulent energy. A most amplified scale, characteristic of the energy containing eddies within the coherent substructure and commensurate with the boundary-layer thickness, was detected. Penetration of the amplified turbulence into the cylinder boundary layer led to the retardation of separation and to a concurrent decrease in the drag coefficient at subcritical cylinder-diameter Reynolds numbers.

Sadeh, W. Z.↗

NASA NTRS1980

Strouhal number influence on flight effects on jet noise radiated from convecting quadrupoles

The paper reports a complementary extension of previous work to include the high-frequency features reflected in the discussion of the higher Strouhal number influence on flight effects. It is found that, in addition to the usual features of flight effects on noise from ordinary flows, the high Strouhal number flows exhibit some more interesting features which are uniquely characteristic to them. The additional features are as follows: (1) Flight effects are more favorable to hot jets than to cold jets; (2) the higher the Strouhal number of the jet flow, the lesser the forward arc amplification due to flight; (3) as the Strouhal number increases, the peak amplification angle in the forward quadrant and the peak suppression angle in the aft quadrant move toward 90 deg and get closer, thus reducing the amplification exposure to a constricted angular region; (4) the silence zone is disturbed and displaced from its normal position parallel to the jet flow to give rise to multiple crossings of flight curves with the static line; and (5) the occurrence of multiple crossings is a strange phenomenon solely characteristic of high Strouhal number with high subcritical jet flows in flight.

Dash, R.↗

NASA NTRS1981

Gortler vortices and transition in wall boundary layers of two Mach 5 nozzles

The onset of transition in the wall boundary layers of two axisymmetric Mach 5 wind-tunnel nozzles has been measured under conditions of extremely low incident disturbance levels. The range of test unit Reynolds numbers, based on conditions at the nozzle exit, was from 6 x 10 to the 6th power m to 2.5 x 10 to the 7th power m. When the nozzle walls were maintained in a polished and clean condition, transition moved gradually upstream as the test Reynolds number was increased. When transition occurred in the supersonic concave wall region, the values of the local Gortler parameter at transition varied from about 5 to 6, whereas the momentum thickness Reynolds number varied from about 750 to 1050. Oil flow patterns obtained near the exit of the nozzles indicated that Gortler vortices were always present when the wall boundary layers were laminar. Calculations for the growth of Gortler vortices based on new results from linear theory for supersonic flat-plate profiles gave amplification ratios to transition from e to the 4th power to e to the 15th power. Possible reasons for this wide range in amplification ratios are discussed, but no definite conclusions are yet possible regarding the values of n in a simple e to the nth power type theory for the assumed linear amplification of Gortler vortices to transition in supersonic nozzles.

Beckwith, I. E.↗

NASA NTRS1981

Jet oscillations caused by vorticity interactions with shock waves

A linear theory is developed for the amplification of disturbances along a jet containing shock waves. The theory indicates that near grazing angles (i.e., wave angles near 90 deg) horizontal vorticity is greatly amplified after passing through the two shock waves that exist in a shock cell. The cumulative amplification and the mode that is amplified most can be obtained if the changes in shock parameters from cell to cell are known. Rapid rates of growth of disturbances are exhibited by shadowgraphs and rates of angular displacement of about 10 are observed. The linear two-dimensional theory also indicates that such rates of amplification occur, and that the behavior of a two-dimensional jet is qualitatively similar to that of a round jet.

Parthasarathy, S. P.↗

NASA NTRS1982

Tone-excited jet: Theory and experiments

A detailed study to understand the phenomenon of broadband jet-noise amplification produced by upstream discrete-tone sound excitation has been carried out. This has been achieved by simultaneous acquisition of the acoustic, mean velocity, turbulence intensities, and instability-wave pressure data. A 5.08 cm diameter jet has been tested for this purpose under static and also flight-simulation conditions. An open-jet wind tunnel has been used to simulate the flight effects. Limited data on heated jets have also been obtained. To improve the physical understanding of the flow modifications brought about by the upstream discrete-tone excitation, ensemble-averaged schlieren photographs of the jets have also been taken. Parallel to the experimental study, a mathematical model of the processes that lead to broadband-noise amplification by upstream tones has been developed. Excitation of large-scale turbulence by upstream tones is first calculated. A model to predict the changes in small-scale turbulence is then developed. By numerically integrating the resultant set of equations, the enhanced small-scale turbulence distribution in a jet under various excitation conditions is obtained. The resulting changes in small-scale turbulence have been attributed to broadband amplification of jet noise. Excellent agreement has been found between the theory and the experiments. It has also shown that the relative velocity effects are the same for the excited and the unexcited jets.

Ahuja, K. K.↗

NASA NTRS1984

Jet noise modification by the 'whistler nozzle'

The farfield noise characteristics of a subsonic whistler nozzle jet are measured as a function of Mach number (0.25, 0.37, and, 0.51), emission angle, and excitation mode. It is shown that a whistler nozzle has greater total and broadband acoustic power than an excited contraction nozzle; and that the intensity of far-field noise is a function of emission angle, Mach number, and whistler excitation stage. The whistler nozzle excitation produces broadband noise amplification with constant spectral shape; the broadband noise amplification (without associated whistler tones and harmonics) increases omnidirectionally with emission angle at all Mach numbers; and the broadband amplification factor decreases as Mach number and emission angle increase. Finally the whistler nozzle is described as a very efficient but inexpensive siren with applications in not only jet excitation but also acoustics.

Hasan, M. A. Z.↗

NASA NTRS1985

Far-field noise of a subsonic jet under controlled excitation

The role of large-scale coherent structures in broadband jet noise suppression and amplification under controlled excitation are studied experimentally. It is found that suppression occurs only at low Reynolds and Mach numbers, when the boundary layer at the jet exit is laminar; the optimum St(Theta) (St, based on the initial shear-layer momentum thickness) value is 0.017, at which the excitation results in a quick roll-up and transition of the laminar shear-layer vortices, yielding coherent structures similar to those existing at high speeds. At the asymptotic level the broadband jet noise can only be amplified by the excitation. The amplification is inferred to be maximum for excitation in the St(D) (St based on the jet diameter) range of 0.65-0.85. Finally, it is noted that the pairing process induced by the excitation is at the origin of the broadband noise amplification.

Zaman, K. B. M. Q.↗

NASA NTRS1986

The influrence of an optical receiving system on statistical characteristics of a lidar signal

The effects connected with correlation of direct and backward waves propagating through the same randomly inhomogeneous media can be observed along the path with refection in a turbulent atmosphere. In particular, the mean intensity of the reflected wave can increase in comparison with the wave propagating in the forward direction at a doubled distance; the intensity fluctuations can become stronger. These effects depend on the strength of optical turbulence, as well as on the diffraction sizes of the exit apertures of the source and the reflector. However, the focusing of radiation reflected with a receiving telescope leads, in some cases, to the fact that the dependence of amplification effects on the parameters becomes essentially different. This should be taken into account when alayzing the lidar signals. The effect of backscattering amplification and amplification of the intensity fluctuations is discussed.

Zuev, A. E.↗