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At least 433 records · Page 24

Machine learning to predict biomass sorghum yields under future climate scenarios

Crop yield modeling is critical in the design of national strategies for agricultural production, particularly in the context of a changing climate. Forecasting yields of bioenergy crops at fine spatial resolutions can help to evaluate near-term and long-term pathways for scaling up bio-based fuel and chemical production, and for understanding the impacts of abiotic stressors such as severe droughts and temperature extremes on potential biomass supply. In this work we used a large dataset of 28,364 Sorghum bicolor yield samples (uniquely identified by county and year of observation), environmental variables, and multiple approaches to analyze historical trends in sorghum productivity across the USA. We selected the most accurate machine learning approach (a variation of the random forest approach) to predict future trends in sorghum yields under four greenhouse gas (GHG) emission scenarios and two irrigation regimes. We identified irrigation practices, vapor pressure deficit, and time (a proxy for technological improvement) as the most important predictors of sorghum productivity. Our results showed a decreasing trend of sorghum yields over future years (on average 2.7% from 2018 to 2099), with greater decline under a high GHG emissions scenario (3.8%) and in the absence of irrigation (4.6%). Geographically, we observed the steepest predicted declines in the Great Lakes (8.2%), Upper Midwest (7.5%), and Heartland (6.7%) regions. Our study demonstrates the use of machine learning to identify environmental controllers of sorghum biomass yield and predict yields with reasonable accuracy. These results can inform the development of more realistic biomass supply projections for bioenergy if sorghum production is scaled up. (c) 2020 Society of Chemical Industry and John Wiley & Sons, Ltd

09 BIOMASS FUELS↗

An analytical method for identifying synergies between behind-the-meter battery and thermal energy storage

Electric utilities build generation capacity to meet the highest demand period, and they often pass on the costs associated with these peaking generators to building owners through demand charges. Building owners can minimize these demand charges by shifting energy use away from peak periods with behind-the-meter storage. This storage can include batteries, which can directly shift the metered load, or thermal energy storage, which can shift thermal-driven electric loads like air conditioning. However, there is a lack of research on how best to combine battery and thermal energy storage. In this study, we develop an analytical sizing method to calculate the potential demand reduction and annualized cost savings for different combinations of thermal and battery energy storage sizes. We show that adding batteries to a thermal energy storage system can increase the total system's load shaving potential. This is particularly true when the building has onsite photovoltaic generation or electric vehicle charging, which add significant variability to the load shape. We also show that for a given total storage size, selecting a higher fraction of thermal energy storage can significantly lower the cycling of the battery, and therefore extend the battery life. This, combined with the expected lower first cost of thermal energy storage materials compared to batteries, shows that hybrid energy storage systems can outperform a standalone battery or standalone thermal storage system. Assuming the thermal storage has a capital cost 6x lower than the battery, our analysis shows that the optimal system is 71% thermal energy storage and 29% battery energy storage for a scenario with electric vehicle charging. The annualized cost savings for this system are $48.6 k/yr, whereas an equivalently sized standalone thermal energy storage system would provide annualized cost savings of $28.5 k/yr and a standalone battery would lead to savings of $8.72 k/yr. The hybrid system also reduces battery cycling by 52% compared to a standalone battery, extending battery lifetime.

25 ENERGY STORAGE↗

Metal additive manufacturing simulation across length, time, and computing scales

Metal additive manufacturing (AM) offers a unique opportunity for production of advanced materials and complex geometries. However, variability in microstructure and properties challenges conventional approaches to design, process optimization, qualification, and materials selection. Modeling and simulation can improve understanding of AM processing and materials, but also poses major challenges for existing computational methods. Simultaneously, modern scientific computing hardware has become increasingly complex, most notably with the adoption of hybrid architectures such as Graphical Processing Units (GPUs). If appropriately utilized, emerging computational capabilities provide an opportunity to reveal new insight into AM processing and the resulting material structure and properties. In this review we describe the computational AM landscape, identify critical gaps, and highlight opportunities to impact the development and application of AM. First, the requirements and challenges of representative AM problem statements will be defined. Here, these problems range from scientific studies to industrial applications and are designed to capture the breadth of challenges facing the AM community. Next, the current state of AM modeling and simulation is evaluated, broken down by enabling hardware and software, process simulation, microstructure simulation, and property simulation. Each section describes the diversity of simulation approaches and associated trade-offs in physical fidelity and computational expense. Each area is then assessed based on their suitability and readiness for current and developing computational architectures. Lastly, the greatest opportunities for future research and application are highlighted, including gaps in modeling capabilities, opportunities for near-term application, and key scientific challenges.

additive manufacturing↗

SEPATH: benchmarking the search for pathogens in human tissue whole genome sequence data leads to template pipelines

Background: Human tissue is increasingly being whole genome sequenced as we transition into an era of genomic medicine. With this arises the potential to detect sequences originating from microorganisms, including pathogens amid the plethora of human sequencing reads. In cancer research, the tumorigenic ability of pathogens is being recognized, for example, Helicobacter pylori and human papillomavirus in the cases of gastric non-cardia and cervical carcinomas, respectively. As of yet, no benchmark has been carried out on the performance of computational approaches for bacterial and viral detection within host-dominated sequence data. Results: We present the results of benchmarking over 70 distinct combinations of tools and parameters on 100 simulated cancer datasets spiked with realistic proportions of bacteria. mOTUs2 and Kraken are the highest performing individual tools achieving median genus-level F1 scores of 0.90 and 0.91, respectively. mOTUs2 demonstrates a high performance in estimating bacterial proportions. Employing Kraken on unassembled sequencing reads produces a good but variable performance depending on post-classification filtering parameters. These approaches are investigated on a selection of cervical and gastric cancer whole genome sequences where Alphapapillomavirus and Helicobacter are detected in addition to a variety of other interesting genera. Conclusions: We provide the top-performing pipelines from this benchmark in a unifying tool called SEPATH, which is amenable to high throughput sequencing studies across a range of high-performance computing clusters. SEPATH provides a benchmarked and convenient approach to detect pathogens in tissue sequence data helping to determine the relationship between metagenomics and disease.

Viral↗

Long-Term Infrasound Sensor Calibration and Characterization

Previous testing has shown that infrasound sensors deployed in the field can exhibit notable deviations from their nominal, lab-based calibrations. These variations may be due to changes in environmental conditions, long-term sensor drift, or other unresolved features. In early 2018, we installed two identical infrasound elements with five infrasound sensors at each element (Chaparral M50A, Chaparral M64LN, CEA/Martec MB2005, CEA/Seismowave MB3a, and Hyperion IFS-5113A). These sensors were accepted or under consideration for use in the International Monitoring System network of the Comprehensive Nuclear-Test-Ban Treaty. Each element had all sensors connected to a single digitizer and port to the atmosphere. We also recorded instrument enclosure air temperature and humidity and external air temperature. Using the MB2005 as the reference, we examine the relative sensor response (both magnitude and phase) as a function of time and frequency and compare it with quarterly laboratory calibrations and environmental conditions. Here, we find that the magnitude response for all sensors exhibits some variability in both the lab and field, with the amplitude variations often >5%. The field-based variations are more severe and occur on both long-term (months) and short-term (hours) timescales. Short-term variability correlates with changes in environmental conditions and is considerable (up to 25%) for the Chaparral M50A and noticeable (∼5%) for the French Alternative Energies and Atomic Energy Commission (CEA) MB3a. Long-term magnitude variability for the Chaparral M50A was up to 50% and does not clearly correlate with environmental conditions. The other sensors show some long-term magnitude offsets, but they have relatively stable responses in the conditions we examined. The MB3a also displays some frequency-dependent magnitude variability and shows a minor dependence on temperature. Phase estimates are stable and near zero for all sensors tested. These results strongly suggest sensor response and variability due to environmental conditions should be considered in future infrasound data interpretation and sensor selection and development.

Fee, David↗

A Machine-Learning Decision-Support Tool for Travel-Demand Modeling

Utility maximization(UM) models are the lifeblood of virtually all travel demand models (TDM) in practice. Be it the traditional travel demand models or more advanced activity-based models, utility maximization models are used extensively to model and predict myriad travel choices such as location choice, mode choice, route choice etc. More recently machine learning (ML) models are being applied in a variety of contexts to predict choice patterns (product suggestions on Amazon, restaurant suggestions on Yelp etc.,). In the TDM arena, there has been interest in incorporating ML models where they can enhance prediction accuracy. Though there have been sporadic efforts at comparing specific utility maximization models to machine learning models, there is a need for a standard comparison tool which can evaluate ML models against UM models for a given choice context. Addressing this need, we present a tool for applying an array of models including logit, nested logit, neural network, Naive Bayes and decision tree classifiers. The tool is specifically tailored to aid in the deciding the best model for a given choice context and can be used to choose an appropriate model family or to construct a model ensemble to improve upon current modeling standards in travel demand modeling. We test our proposed system on household vehicle count and work schedule targets from the 2017 National Household Travel Survey. Results demonstrate that for some variables, logit are not the most effective models, and the proposed system can aid in selecting a better model.

33 ADVANCED PROPULSION SYSTEMS↗

The brown dwarf population in the star-forming region NGC 2264

ABSTRACT The brown dwarf population in the canonical star-forming region NGC 2264 is so far poorly explored. We present a deep, multiwavelength, multiepoch survey of the star-forming cluster NGC 2264, aimed to identify young brown dwarf candidates in this region. Using criteria including optical/near-infrared (IR) colours, variability, Spitzer mid-IR colour excess, extinction, and Gaia parallax and proper motion (in order of relevance), we select 902 faint red sources with indicators of youth. Within this sample, we identify 429 brown dwarf candidates based on their IR colours. The brown dwarf candidates are estimated to span a mass range from 0.01 to 0.08 M⊙. We find rotation periods for 44 sources, 15 of which are brown dwarf candidates, ranging from 3.6 h to 6.5 d. A subset of 38 brown dwarf candidates show high-level irregular variability indicative of ongoing disc accretion, similar to the behaviour of young stars.

Pearson, Samuel↗

Investigation of Coincidence Counting for Improving Minimal Detectable Activity of 110m Ag in Single Particle Gamma Analysis

Post-irradiation examination (PIE) of fuel particles from the fourth Advanced Gas Reactor Fuel Development and Qualification (AGR) Program irradiation (AGR-5/6/7) is being performed at Oak Ridge National Laboratory (ORNL). Tristructural isotropic (TRISO)-coated particles and associated compacts for the AGR-5/6/7 experiment fabricated by BWX Technologies Nuclear Operations Group were formed into a graphite matrix compact and irradiated at the Advanced Test Reactor at Idaho National Laboratory. At ORNL, particles are deconsolidated from the graphite matrix compact and individually scanned for emitted gamma rays with the Irradiated Microsphere Gamma Analyzer (IMGA). The IMGA system comprises a single high purity germanium (HPGe) detector, an automated particle handling vacuum system, and an ORTEC DSPEC-50 digital spectrometer for gamma ray analysis. IMGA quantifies gamma ray-emitting fission product inventories of individual TRISO particles, and these inventories can be compared with the measured average inventories per particle and radionuclide inventories predicted by AGR-5/6/7 physics calculations to determine if a particle experienced radionuclide release. Details on IMGA data collection methods can be found in the literature. The TRISO particle’s SiC layer provides structural support, as well as a barrier for fission product release during irradiation or subsequent safety testing. A weakened or compromised SiC layer can be identified by the release of radionuclides, such as 137 Cs, which is detected by IMGA. However, select radionuclides, such as 90 Sr, 110 mAg, and 154 Eu have been shown to migrate through an intact SiC layer. Measurement of the radionuclide 110 mAg is significant as its release has been shown to be particularly sensitive to in-reactor conditions (e.g., temperature) with broad variable particle to-particle release behaviors observed within a single compact. As such, 110 mAg activity is often used for particle selection for comprehensive PIE as bounding 110 mAg retention particles are hypothesized to represent limits in particle behaviors within a compact. As TRISO particle fuel PIE activities continue over time, IMGA measurements of the 110 mAg inventory are eventually hindered because of its relatively short half-life (~250 days). As the fuel ages from its end of irradiation (EOI) date, the measurement uncertainty and minimum detectable activity (MDA) of 110m Ag increase because the detector background continuum begins to dominate. For particles from the second AGR irradiation experiment (AGR-2), the 110m Ag MDA was above 20% of the calculated average particle inventory after approximately five half-lives, and 110m Ag activity was no longer measurable with IMGA after approximately seven half-lives. Therefore, coincidence counting approaches have been explored to determine feasibility of leveraging new approaches to overcome limitations associated with increasing MDA over time.

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗

High-Resolution Tropical Channel Model Simulations of Tropical Cyclone Climatology and Intraseasonal-to-Interannual Variability

We tailored a tropical channel configuration of the Weather Research and Forecasting (WRF) Model to study tropical cyclone (TC) activity and associated climate variabilities. This tropical channel model (TCM) covers from 30°S to 50°N at 27-km horizontal resolution, with physics parameterizations carefully selected to achieve more realistic simulations of TCs and large-scale climate mean states. In this work we performed 15-member ensembles of retrospective simulations from 1982 to 2016 hurricane seasons. A thorough comparison with observations demonstrates that the TCM yields significant skills in simulating TC activity climatology and variabilities in each basin, as well as TC physical structures. The correlation of the ensemble averaged accumulated cyclone energy (ACE) with observations in the western North Pacific (WNP), eastern North Pacific (ENP), and North Atlantic (NAT) is 0.80, 0.64, and 0.61, respectively, but is insignificant in the north Indian Ocean (NIO). Moreover, the TCM-simulated modulations of El Niño–Southern Oscillation (ENSO) and the Madden–Julian oscillation (MJO) on the large-scale environment and TC genesis also agree well with observations. To examine the TCM’s potential for seasonal TC prediction, the model is used to forecast the 2017 and 2018 hurricane seasons, using bias-corrected sea surface temperatures (SSTs) from the CFSv2 seasonal prediction results. The TCM accurately predicts the hyperactive 2017 NAT hurricane season and near-normal WNP and ENP hurricane seasons when initialized in May. In addition, the TCM accurately predicts TC activity in the NAT and WNP during the 2018 season, but underpredicts ENP TC activity, in association with a poor ENSO forecast.

54 ENVIRONMENTAL SCIENCES↗

Investigation of Dynamics in BMIM TFSA Ionic Liquid through Variable Temperature and Pressure NMR Relaxometry and Diffusometry

A comprehensive variable temperature, pressure and frequency multinuclear ( 1 H, 2 H, and 19 F) magnetic resonance study was undertaken on selectively deuterated 1-butyl-3-methylimidazolium bis(trifluoromethylsulfonyl)amide (BMIM TFSA) ionic liquid isotopologues. This study builds on our earlier investigation of the effects of increasing alkyl chain length on diffusion and dynamics in imidazolium-based TFSA ionic liquids. Fast field cycling 1 H T 1 data revealed multiple modes of motion. Through calculation of diffusion coefficient (D) values and activation energies, the low- and high-field regimes were assigned to the translational and reorientation dynamics respectively. Variable-pressure 2 H T 1 measurements reveal site-dependent interactions in the cation with strengths in the order MD 3 > CD 3 > CD 2 , indicating dissimilarities in the electric field gradients along the alkyl chain, with the CD 2 sites having the largest gradient. Additionally, the α saturation effect in T 1 vs. P was observed for all three sites, suggesting significant reduction of the short-range rapid reorientational dynamics. This reduction was also deduced from the variable pressure 1 H T 1 data, which showed an approach to saturation for both the methyl and butyl group terminal methyl sites. Pressure-dependent D measurements show independent motions for both cations and anions, with the cations having greater D values over the entire pressure range.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Synthesis and analysis of separation processes for extracellular chemicals generated from microbial conversions

Recent advances in metabolic engineering have enabled the production of chemicals via bio-conversion using microbes. However, downstream separation accounts for 60–80% of the total production cost in many cases. Previous work on microbial production of extracellular chemicals has been mainly restricted to microbiology, biochemistry, metabolomics, or techno-economic analysis for specific product examples such as succinic acid, xanthan gum, lycopene, etc. In these studies, microbial production and separation technologies were selected apriori without considering any competing alternatives. However, technology selection in downstream separation and purification processes can have a major impact on the overall costs, product recovery, and purity. To this end, we apply a superstructure optimization based framework that enables the identification of critical technologies and their associated parameters in the synthesis and analysis of separation processes for extracellular chemicals generated from microbial conversions. We divide extracellular chemicals into three categories based on their physical properties, such as water solubility, physical state, relative density, volatility, etc. We analyze three major extracellular product categories (insoluble light, insoluble heavy and soluble) in detail and provide suggestions for additional product categories through extension of our analysis framework. The proposed analysis and results provide significant insights for technology selection and enable streamlined decision making when faced with any microbial product that is released extracellularly. The parameter variability analysis for the product as well as the associated technologies and comparison with novel alternatives is a key feature which forms the basis for designing better bioseparation strategies that have potential for commercial scalability and can compete with traditional chemical production methods.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Variable-speed supercharger for highly diluted internal combustion engines

An engine control system includes an internal combustion engine including a plurality of cylinders. An intake manifold is connected to the internal combustion engine. An exhaust manifold is connected to the internal combustion engine. A supercharger is connected to an air intake passage and the intake manifold and includes a variable speed drive. A throttle valve is disposed in the air intake passage. An exhaust passage is in connection with the exhaust manifold. A passive selective catalytic reduction catalyst system is in communication with the exhaust passage. An exhaust gas recirculation passage is in communication with the exhaust passage and the air intake passage and includes an exhaust gas recirculation valve. A controller controls the variable speed drive of the supercharger and the throttle valve and the exhaust gas recirculation valve based upon engine conditions.

42 ENGINEERING↗

Methods of making a component with variable magnetization and related components

A method of forming a component having a variation in saturation magnetization is presented. The method includes selectively diffusing nitrogen into a metallic component of a masked metallic component by exposing the masked metallic component to a nitrogen-rich atmosphere. The masked metallic component includes a patterned oxide layer formed on a surface of the metallic component, and the patterned oxide layer includes an oxide of a metal present in the metallic component. A related component is also presented.

36 MATERIALS SCIENCE↗

THE INFLUENCE OF CHANGES IN NUCLEAR COVARIANCE DATA ON THE CALCULATION OF Ck FOR HIGHLY ENRICHED URANIUM SOLUTION SYSTEMS

Since their initial development nearly 20 years ago, sensitivity/uncertainty (S/U) techniques have been increasingly applied to criticality safety validation. These techniques can generally be applied quickly and for a range of purposes after the initial investment of calculating sensitivities for a safety analysis model and a range of potential benchmark experiments. This paper discusses these applications and how they have been affected by changes in covariance data libraries.Critical experiment selection is the most prominent area in which S/U methods impact validation efforts. These methods allow for rigorous calculation of similarity between safety analysis models and potential benchmarks. The most widely used of these methods is the integral parameter ck, although other integral parameters such as E and some non-integral parameters have also been explored. The ck parameter calculates the fraction of nuclear data–induced uncertainty shared between an application and an experiment; this metric is predicated on the assessment that nuclear data errors are the most likely source of computational bias. Therefore, values of ck are inherently dependent on the nuclear covariance library used to calculate them and inevitably change when these data change.Past studies have shown how much the ck values can vary, especially in difficult validation cases like burnup credit. These significant changes are the result of differences in the relative uncertainties of nuclides with large sensitivities, and they imply differences in benchmark applicability to the safety analysis model. This paper demonstrates that more common criticality safety models involving single fissile species have significantly less variability of ck between covariance libraries, thus enhancing confidence in the application of S/U methods for experiment selection in these scenarios. This paper presents case studies examining the impact of these changes on some hypothetical safety analysis systems.

Clarity, Justin↗

First Measurement of Differential Charged Current Quasielastic-like $\nu_\mu$–Argon Scattering Cross Sections In Kinematic Imbalance Variables With The MicroBooNE Detector

We report the first measurement of flux-integrated multi-differential cross sections for charged-current events with muon neutrinos scattering on argon with solely a muon and a single proton in the final state as a function of kinematic imbalance variables. The measurement was carried out using the Booster Neutrino Beam at Fermi National Accelerator Laboratory within the MicroBooNE Liquid Argon Time Projection Chamber detector with an exposure of 6.79 × 10 20 protons on target. Events were selected to enhance the contribution of charged-current mesonless interactions with one proton detected in the final state. The data discussed here are reported in terms of multidifferential cross sections in kinematic imbalance variables, which are generally sensitive to nuclear effects. The double-differential results in these variables can provide an excellent handle to disentagle specific nuclear aspects not easily isolated via single differential cross sections. Our results pave a path towards identifying regions of the phase-space where future interaction modeling development and Monte Carlo neutrino generator tuning efforts should concentrate. EVENT SELECTION Neutrino oscillation measurements aim to extract neutrino mixing angles, mass differences, the value of the chargeparity violating phase in the lepton sector, and to search for new physics beyond the Standard Model [1, 2]. For that to be achieved, an unprecedented understanding of neutrino-argon interactions is of utmost importance since a growing number of neutrino oscillation experiments employ Liquid Argon Time Projector Chamber (LArTPC) neutrino detectors [3–6]. The accuracy to which these experiments can extract neutrino oscillation parameters requires a good understanding of the neutrino energy. Experimentally, this energy is deduced from the measured kinetic energies of particles that are emitted following the neutrino interaction in the detector. The kinematic properties of such finalstate particles reflect complex dynamics due to nuclear and initial-state effects of the interaction [7]. However, certain categories of nuclear effects can be isolated by variables built specifically to characterize the degeneracy between such effects [8–10]. This note reports cross sections in kinematic variables sensitive to nuclear effects using events with one detected muon with momentum 0.1 < pµ < 1.2 GeV/c, and exactly one proton with 0.3 < pp < 1 GeV/c. This signal definition includes events with any number of protons below 300 MeV/c, neutrons at any momenta, and charged pions with momentum lower than 70 MeV/c. This choice is guided by the fact that their experimental signature of correlated muon-proton pairs is fairly straightforward to reconstruct [11–22]. Such events primarily originate from chargedcurrent (CC) neutrino-nucleon quasielastic (QE) scattering interactions where the neutrino removes a single intact nucleon from the nucleus without producing any additional particles. This definition can also include contributions from interactions that lead to the production of additional particles that are absent from the final state due to nuclear effects, such as pion absorption, or have momenta that are below the experimental detection threshold.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

Optimizing event selection with the random grid search

In this paper, the random grid search (RGS) is a simple, but efficient, stochastic algorithm to find optimal cuts that was developed in the context of the search for the top quark at Fermilab in the mid-1990s. The algorithm, and associated code, have been enhanced recently with the introduction of two new cut types, one of which has been successfully used in searches for supersymmetry at the Large Hadron Collider. The RGS optimization algorithm is described along with the recent developments, which are illustrated with two examples from particle physics. One explores the optimization of the selection of vector boson fusion events in the four-lepton decay mode of the Higgs boson and the other optimizes SUSY searches using boosted objects and the razor variables.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

Guidelines and considerations for designing field experiments simulating precipitation extremes in forest ecosystems

1. Precipitation regimes are changing in response to climate change, yet understanding of how forest ecosystems respond to extreme droughts and pluvials remains incomplete. As future precipitation extremes will likely fall outside the range of historical variability, precipitation manipulation experiments (PMEs) are critical to advancing knowledge about potential ecosystem responses. However, few PMEs have been conducted in forests compared to short-statured ecosystems, and forest PMEs have unique design requirements and constraints. Moreover, past forest PMEs have lacked coordination, limiting cross-site comparisons. Here, we review and synthesize approaches, challenges, and opportunities for conducting PMEs in forests, with the goal of guiding design decisions, while maximizing the potential for coordination. 2. We reviewed 63 forest PMEs at 70 sites world-wide. Workshops, meetings, and communications with experimentalists were used to generate and build consensus around approaches for addressing the key challenges and enhancing coordination. 3. Past forest PMEs employed a variety of study designs related to treatment level, replication, plot and infrastructure characteristics, and measurement approaches.Important considerations for establishing new forest PMEs include: selecting appropriate treatment levels to reach ecological thresholds; balancing cost, logistical complexity, and effectiveness in infrastructure design; and preventing unintended water subsidies. Response variables in forest PMEs were organized into three broad tiers reflecting increasing complexity and resource intensiveness, with the first tier representing a recommended core set of common measurements. 4. Differences in site conditions combined with unique research questions of experimentalists necessitate careful adaptation of guidelines for forest PMEs to balance local objectives with coordination among experiments. We advocate adoption of a common framework for coordinating forest PME design to enhance cross-site comparability and advance fundamental knowledge about the response and sensitivity of diverse forest ecosystems to precipitation extremes.

Climate Extremes, drought, ecological thresholds, ↗

Population genomics of the eastern cottonwood ( Populus deltoides )

Abstract Despite its economic importance as a bioenergy crop and key role in riparian ecosystems, little is known about genetic diversity and adaptation of the eastern cottonwood ( Populus deltoides ). Here, we report the first population genomics study for this species, conducted on a sample of 425 unrelated individuals collected in 13 states of the southeastern United States. The trees were genotyped by targeted resequencing of 18,153 genes and 23,835 intergenic regions, followed by the identification of single nucleotide polymorphisms ( SNP s). This natural P. deltoides population showed low levels of subpopulation differentiation ( F ST = 0.022–0.106), high genetic diversity (θ W = 0.00100, π = 0.00170), a large effective population size ( N e ≈ 32,900), and low to moderate levels of linkage disequilibrium. Additionally, genomewide scans for selection (Tajima's D ), subpopulation differentiation ( X T X ), and environmental association analyses with eleven climate variables carried out with two different methods ( LFMM and BAYENV 2) identified genes putatively involved in local adaptation. Interestingly, many of these genes were also identified as adaptation candidates in another poplar species, Populus trichocarpa , indicating possible convergent evolution. This study constitutes the first assessment of genetic diversity and local adaptation in P. deltoides throughout the southern part of its range, information we expect to be of use to guide management and breeding strategies for this species in future, especially in the face of climate change.

54 ENVIRONMENTAL SCIENCES↗