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At least 433 records · Page 24
All sourcesNASA NTRSDOE OSTI
DOE OSTI2024

Visualization of lignification in flax stem cell walls with novel click-compatible monolignol analogs

As an essential part of plant cell walls, lignin provides mechanical support for plant growth, enhances water transport, and helps to defend against pathogens. As the most abundant natural aromatic-based renewable resource on earth, its biosynthesis has always been a research focus, and it is still currently under study. In this study, the p-coumaryl alcohol analog (H ALK ) and the coniferyl alcohol analog (G ALK ) containing an alkyne group at the ortho position were synthesized and applied to lignification in vivo and in vitro. The incorporation of these novel lignin monomers was observed via fluorescence imaging. It was found that the two monolignol analogs could be incorporated in dehydrogenated polymers (DHPs) in vitro and in flax cell walls in vivo. The results showed that as the cultivation time and precursor concentration varied, the deposition of H and G-type lignin exhibited differences in deposition mode. At the subcellular scale, the deposited lignin first appears in the cell corner and the middle lamella, and then gradually appears on the cell walls. Furthermore, lignin was also found in bast fiber. It was demonstrated that these new molecules could provide high-resolution localization of lignin during polymerization.

59 BASIC BIOLOGICAL SCIENCES↗
NASA NTRS1972

Stem clutch for motor driven valve

Development of mechanical device to reduce possibility of damage to motor driven needle valve is discussed. Mechanical clutch is employed to allow slippage when needle valve reaches limit of travel. Operation of system for various conditions is described.

Blum, D. E.↗
NASA NTRS1974

Studies on Auditory and Vestibular End Organs and Brain Stem Nuclei

Cats were exposed to tones of 125, 1000, 2000, and 4000 Hz at sound pressure levels in the range 120 to 157.5 db, and for durations of one hour (1000, 2000, 4000 Hz) or four hours (125 Hz). Pure tone audiograms were obtained for each animal before and after exposure. Cochleas of animals were examined by phase-contrast microscopy. Extent of inner ear damage and range of frequencies for which hearing loss occurred increased as exposure tone was decreased in frequency. For example, exposure to 4000 Hz produced damage in a restricted region of the cochlea and hearing loss for a relatively narrow range of frequencies; exposure to 125 Hz produced wide-spread inner ear damage and hearing loss throughout the frequency range 125 to 6000 Hz.

Ades, H. W.↗
NASA NTRS1979

Variational description of the positive column with two-stem ionization

The ionization balance in diffusion dominated discharges which depends on both one and two step ionization processes is considered. The Spenke diffusion equation (D sq delta n + neutrino n + sq kn =0) describing such conditions is solved by the Rayleigh-Ritz variational method. Simple analytic approximations to the density profile, and the similarity relation between neutrino,k,D and the discharge dimensions, are derived for planar and cylindrical geometry, and compared with exact computations for certain limiting cases.

Crawford, F. W.↗