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At least 433 records · Page 24
All sourcesNASA NTRSDOE OSTI
DOE OSTI2024

Leptothrix ochracea genomes reveal potential for mixotrophic growth on Fe(II) and organic carbon

ABSTRACT Leptothrix ochracea creates distinctive iron-mineralized mats that carpet streams and wetlands. Easily recognized by its iron-mineralized sheaths, L. ochracea was one of the first microorganisms described in the 1800s. Yet it has never been isolated and does not have a complete genome sequence available, so key questions about its physiology remain unresolved. It is debated whether iron oxidation can be used for energy or growth and if L. ochracea is an autotroph, heterotroph, or mixotroph. To address these issues, we sampled L. ochracea -rich mats from three of its typical environments (a stream, wetlands, and a drainage channel) and reconstructed nine high-quality genomes of L. ochracea from metagenomes. These genomes contain iron oxidase genes cyc2 and mtoA, showing that L. ochracea has the potential to conserve energy from iron oxidation. Sox genes confer potential to oxidize sulfur for energy. There are genes for both carbon fixation (RuBisCO) and utilization of sugars and organic acids (acetate, lactate, and formate). In silico stoichiometric metabolic models further demonstrated the potential for growth using sugars and organic acids. Metatranscriptomes showed a high expression of genes for iron oxidation; aerobic respiration; and utilization of lactate, acetate, and sugars, as well as RuBisCO, supporting mixotrophic growth in the environment. In summary, our results suggest that L. ochracea has substantial metabolic flexibility. It is adapted to iron-rich, organic carbon-containing wetland niches, where it can thrive as a mixotrophic iron oxidizer by utilizing both iron oxidation and organics for energy generation and both inorganic and organic carbon for cell and sheath production. IMPORTANCE Winogradsky's observations of L. ochracea led him to propose autotrophic iron oxidation as a new microbial metabolism, following his work on autotrophic sulfur-oxidizers. While much culture-based research has ensued, isolation proved elusive, so most work on L. ochracea has been based in the environment and in microcosms. Meanwhile, the autotrophic Gallionella became the model for freshwater microbial iron oxidation, while heterotrophic and mixotrophic iron oxidation is not well-studied. Ecological studies have shown that Leptothrix overtakes Gallionella when dissolved organic carbon content increases, demonstrating distinct niches. This study presents the first near-complete genomes of L. ochracea , which share some features with autotrophic iron oxidizers, while also incorporating heterotrophic metabolisms. These genome, metabolic modeling, and transcriptome results give us a detailed metabolic picture of how the organism may combine lithoautotrophy with organoheterotrophy to promote Fe oxidation and C cycling and drive many biogeochemical processes resulting from microbial growth and iron oxyhydroxide formation in wetlands.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2023

Assessing Methodologies for Detecting Water Intrusion in Wall Systems: Phase 2

Studies by the University of Florida, the Environmental Protection Agency (EPA) and the U.S. Department of Housing (HUD) have revealed that there is a substantial fraction of commercial and residential buildings that have been exposed to moisture resulting in damage or durability problems. Water intrusion into building envelope components leads to a variety of undesirable conditions such as mold, wood rot, corrosion, and aesthetic damage. Tests methods that are presently used to evaluate the amount of water intrusion into a building envelope component are usually qualitative in nature. For example, ASTM E 331, Standard Test Method for Water Penetration of Exterior Windows, Curtain Walls, and Doors by Uniform Static Air Pressure Difference requires that you “observe and record points of water leakage, if any.” This test was originally developed to assess the performance of fenestration products but is commonly adapted to evaluate other enclosure assemblies. However, when it is typically used for walls, this procedure is limited to recognizing if the moisture is visually observable from the backside side of the sheathing. It does not address moisture that is absorbed in the layers of the building envelope component, which could impact the durability of the assembly. Clearly a quantitative means of determining water penetration would improve the quality of this type of test and assist with better understanding the resultant impact on enclosure assemblies. In 2018-20, Oak Ridge National Laboratory, in conjunction with the Air Barrier Association of America, initiated a research project to address this issue. The purpose of that study was to evaluate nine different methods of detecting moisture intrusion through a wall assembly. air and water barrier. The wall assemblies included metal frame construction faced with gypsum sheathing and both self-adhered and fluid applied air and water barriers (AWB) were evaluated for this exercise. This project did not test the efficacy of the different AWBs, rather, fasteners were purposely installed in various ways to foster water penetration and activate the different methods of detection. Each detection method was evaluated for five features that included simplicity of use, cost of implementation, whether the method was quantitative or subjective, accuracy, and applicability. A scale of green/yellow/red was used to assess each feature where green was acceptable, yellow was borderline, and red was not to be pursued at this time. This report covers additional research that has been undertaken to extend the activities initiated in this earlier project with refinements for specific detection methods and considerations for expansion related to field versus laboratory testing standards.

42 ENGINEERING↗
DOE OSTI2024

LLNL FESP Theory Highlights: November 2024

This talk presented a refined understanding of plasma-facing thermionic emitter cathodes by examining the limitations of the conventional space charge-limited model, which overlooks ion trapping dynamics within the virtual cathode. While conventional theory distinguishes between temperature-limited and space charge-limited regimes based on electron current constraints, recent findings reveal that trapped ions can form a quasi-neutral "second plasma," expanding upstream and interacting with the primary plasma. These effects are amplified in non-planar geometries, such as cylindrical or spherical sheaths, where trapped ions create a positive feedback loop with the upstream plasma. To address these phenomena, a new "Aid-and-Compete" model was developed, offering insights into current enhancement and sheath mode transitions relevant for plasma devices, including emissive cathodes in electric propulsion, fusion applications, thin film deposition systems, and plasma diagnostics.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗
DOE OSTI2023

Testing Various Cement Formulations under Temperature Cycles and Drying Shrinkage for Low-Temperature Geothermal Wells

Low-enthalpy geothermal wells are considered a sustainable energy source, particularly for district heating in the Netherlands. The cement sheath in these wells experiences thermal cycles. The stability of cement recipes under such conditions is not well understood. In this work, thermal cycling experiments for intermediate- and low-temperature geothermal well cements have been conducted. The samples were cured either under ambient conditions or under realistic pressure and temperature for 7 days. The samples did not show any signs of failure after performing 10 cycles of thermal treatment between 100 °C and 18 °C. We also tested cement formulations under drying conditions. Drying shrinkage is caused by a reduction in the water content of cement, which leads to capillary forces that can damage cement. Such circumstances lead to tensile stresses causing radial cracks. Most samples exhibited cracks under low humidity conditions (drying). Fiber reinforcement, especially using short PP fibers, improved the cement’s resilience to temperature and humidity changes. Such additives can improve the longevity of cement sheaths in geothermal wells.

36 MATERIALS SCIENCE↗
DOE OSTI2024

On the mechanisms of J c increment and degradation in high- J c Ba122 tapes made by different processing methods

We compared the grain and grain boundary (GB) nanostructures in two Ba122 tapes with similarly high J c . The Ag-sheathed tape made by hot pressing has larger, more plate-like grains with better c-axis alignment but has more GBs blocked by FeAs and Ba–O. In contrast, the tape made by cold pressing with an Ag-Sn/stainless steel sheath possesses fewer plate-like grains and weaker grain alignment but has more continuous current paths with clean physically well-connected GBs. Our nanostructural comparison emphasizes the strong need to achieve both good grain alignment and clean GBs for further J c improvement of Ba122 tapes.

Kametani, F.↗
DOE OSTI2020

NGC 4869 in the Coma Cluster: Twist, Wrap, Overlap, and Bend

The upgraded Giant Metrewave Radio Telescope (GMRT) has been used to image the head–tail radio galaxy NGC 4869 in the Coma cluster with an angular resolution of 6.″26 at 250–500 MHz and 2.″18 at the 1050–1450 MHz bands. The archival legacy GMRT data have also been used to image the source with angular resolutions from 4.″9 to 21.″8 at 610 MHz, 325 MHz, 240 MHz, and 150 MHz. We find that the ∼200 kpc scale radio morphology consists of five distinct regions with the clear presence of a pinch at ≈1.′4 (=38.8 kpc) and a ridge at ≈3.′4 (=94.2 kpc) from the head. The sharp bend by ∼70° at ∼3.′5 (=97 kpc) from the head is possibly due to projection effects. The radio spectra show progressive spectral steepening as a function of distance from the head and there is possibly re-acceleration of the synchrotron electrons and perhaps also magnetic field regeneration in the 6″–208″ (=2.8–96.1 kpc) region of the jet. We report a steep spectrum sheath layer enveloping a flat spectrum spine, hinting at a transverse velocity structure with a fast-moving spine surrounded by a slow-moving sheath layer. We also derive the lifetimes of the radiating electrons and equipartition parameters. A plausible explanation for the characteristic feature, a ridge of emission perpendicular to the direction of tail is the flaring of a straight, collimated radio jet as it crosses a surface brightness edge due to Kelvin–Helmholtz instabilities.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2026

Distortion of a Relativistic Jet Echoing a Magnetic Flux Eruption

Magnetized accretion onto spinning black holes (BHs) can accumulate a large magnetic flux across the event horizon and launch a pair of relativistic jets via the Blandford–Znajek mechanism. In the magnetically saturated (arrested) state, excess magnetic flux is ejected from the BH in episodic magnetic flux eruptions, which result in a significant yet temporary reduction of jet power. We analyze results of a high-resolution 3D general-relativistic magnetohydrodynamic numerical simulation of geometrically thick magnetically saturated accretion onto a high-spin Kerr BH for a single cycle of magnetic flux eruption and accumulation. We show that following an eruption, a weakened jet develops a strong helical distortion with a distinct structure of magnetic fields—the poloidal field along the jet core is unaffected by the eruption; while toroidal field lines, ejected from the BH during the eruption and later readvected onto it, form poloidal “bypasses” along the inner jet sheath. Such a distortion may appear in sources fed by geometrically thick accretion flows as an asymmetric superluminal knot, strongly interacting with the jet sheath along an oblique working surface. The jet section repowered by magnetic flux reaccumulated on the BH is tilted by a few degrees, implying significant variations in radiation boost towards observers of BL Lac blazars. The intrinsic structure of the jet spine is consistent with axisymmetric semi-analytical models.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2021

Reconnection-driven Particle Acceleration in Relativistic Shear Flows

Particle energization in shear flows is invoked to explain nonthermal emission from the boundaries of relativistic astrophysical jets. Yet the physics of particle injection, i.e., the mechanism that allows thermal particles to participate in shear-driven acceleration, remains unknown. With particle-in-cell simulations, we study the development of Kelvin–Helmholtz (KH) instabilities seeded by the velocity shear between a relativistic magnetically dominated electron–positron jet and a weakly magnetized electron–ion ambient plasma. Here, we show that, in their nonlinear stages, KH vortices generate kinetic-scale reconnection layers, which efficiently energize the jet particles, thus providing a first-principles mechanism for particle injection into shear-driven acceleration. Our work lends support to spine-sheath models of jet emission—with a fast core/spine surrounded by a slower sheath —and can explain the origin of radio-emitting electrons at the boundaries of relativistic jets.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2021

Efficacy of a portable, moderate-resolution, fast-scanning differential mobility analyzer for ambient aerosol size distribution measurements

Abstract. Ambient aerosol size distributions obtained with a compact scanning mobility analyzer, the “Spider” differential mobility analyzer (DMA), are compared to those obtained with a conventional mobility analyzer, with specific attention to the effect of mobility resolution on the measured size distribution parameters. The Spider is a 12 cm diameter radial differential mobility analyzer that spans the 10–500 nm size range with 30 s mobility scans. It achieves its compact size by operating at a nominal mobility resolution R=3 (sheath flow = 0.9 L min−1; aerosol flow = 0.3 L min−1) in place of the higher ratio of sheath flow to aerosol flow commonly used. The question addressed here is whether the lower resolution is sufficient to capture key characteristics of ambient aerosol size distributions. The Spider, operated at R=3 with 30 s up- and downscans, was co-located with a TSI 3081 long-column mobility analyzer, operated at R=10 with a 360 s sampling duty cycle. Ambient aerosol data were collected over 26 consecutive days of continuous operation, in Pasadena, CA. Over the 17–500 nm size range, the two instruments exhibit excellent correlation in the total particle number concentrations and geometric mean diameters, with regression slopes of 1.13 and 1.00, respectively. Our results suggest that particle sizing at a lower resolution than typically employed may be sufficient to obtain key properties of ambient size distributions, at least for these two moments of the size distribution. Moreover, it enables better counting statistics, as the wider transfer function for a given aerosol flow rate results in a higher counting rate.

54 ENVIRONMENTAL SCIENCES↗
NASA NTRS1970

Improved shielding termination adapter for electrical cable connectors

Termination adapter replaces braid ring and ensures permanent attachment and grounding of sheath wires. The inner ferrule of the termination is slipped inside the exposed ends of the sheath wires and the outer ferrule is placed over the wires and crimped in place to secure the wires for grounding.

Vanasse, M. A.↗
NASA NTRS1972

Ion heating mechanism in a modified Penning discharge

Ions with Maxwellian energy distributions and kinetic temperatures ranging from 20 eV to 7 keV have been observed in a modified Penning discharge operating in the steady state. Investigation of the plasma revealed two distinct spoke-like concentrations of charge rotating with different velocities in the sheath between the plasma and the anode ring. The faster spoke consists of electrons rotating with the E/B drift velocity, where E is the electric field and B is the magnetic field strength. The slow spoke consists of ions, the thermal velocity of which is observed to be proportional to the spoke velocity. The experimental data are consistent with a model whereby the ion drift velocity in this spoke, corresponding to kilovolt ion energies, is Maxwellianized by strong electrostatic turbulence in the sheath. Theoretical expressions are derived for the frequency of the electron and ion spoke rotation, for the ion kinetic temperature, and for the ion heating efficiency as functions of the discharge parameters. These expressions are shown to be consistent with extensive experimental data.

Roth, J. R.↗
NASA NTRS1974

Calibration of a cylindrical RF capacitance probe

The calibration is considered of an RF antenna capacitance probe carried aboard the RAE-1 spacecraft and the correction of the probe for external effects, believed to be primarily due to local positive ion sheaths and/or photoelectron sheaths surrounding the antenna. The RAE-1 spacecraft was launched in July 1968 into a 5850-km. Circular orbit of 121-degree inclination and carried several antenna and radiometer systems covering a frequency range of 0.2 to 9.2 MHz for radio astronomical studies. The RF capacitance probe measurements discussed utilized a 37-meter electric dipole antenna formed by two monopoles made of silver-coated beryllium-copper alloy tapes formed into hollow cylindrical tubes 1.3 cm in diameter.

Mosier, S. R.↗
NASA NTRS1974

Plasma waves in the dayside polar cusp. II - Magnetopause and polar magnetosheath

During the outbound pass of Nov. 1, 1968, Ogo 5 sporadically encountered the low-altitude polar cusp at low magnetic latitudes. The spacecraft remained in the cusp beyond six earth radii, and it then traversed the interface region between the magnetospheric cusp and the magnetosheath. Two large scale discontinuities were detected in this sheath-cusp transition region, and several possible interpretations are evaluated here. At 1427 UT, local changes in magnetic field orientation and the variation in ULF magnetic power spectral density were typical of shifts detected at the magnetopause, although the spacecraft did not traverse a true boundary of warm plasma at this point. The second discontinuity, detected at 1456 UT, resembled a collisionless shock, and it was characterized by observations of intense, impulsive VLF electric field bursts and rapid local variations in both total ion flux and differential electron flux. The simplest interpretation is that Ogo 5 had traversed a standing shock within the sheath.

Scarf, F. L.↗
NASA NTRS1974

Electric potential on solid spheres in a plasma

Derivation of the general expression for the potential on a solid sphere immersed in a plasma, showing the dependence of the potential on the radius (a) of the sphere and the radius (s) of the plasma sheath that develops around the sphere. In the limit where the radius a is much larger than the sheath thickness s-a, the well-known result for the potential on an infinite wall in contact with a plasma is recovered from this expression. At the other extreme, where s is much larger than a, the result derived by Spitzer (1941) for the potential on spherical grains in the interstellar plasma is obtained. Since the surface of the sphere forms a sink for the charged particles, there is a net drift of the plasma towards the surface. The effect of this drift on the potential is examined. Finally, for very small metallic spheres, an effect leading to a revision of the potential is discussed. This effect consists in a lowering of the potential barrier for the electrons due to the image force. The various effects limiting the potential on spheres are discussed.

De, B. R.↗
NASA NTRS1975

Io-accelerated electrons and ions

A description is given of a model in which the Jupiter moon Io interacts with the Jovian magnetosphere through plasma sheaths in the vicinity of Io. Several recent results available from Pioneer 10 are used to revise a Io sheath model suggested by Gurnett (1972) and developed by Hubbard et al. (1974). The revised model is used to suggest an explanation for a number of observations. Attention is also given to other phenomena which might be detectable with the aid of future experiments.

Shawhan, S. D.↗
NASA NTRS1976

Substorm phenomena and the uniqueness of the geostationary orbit for observation of dynamic processes in the magnetosphere

During the Spring equinox, both ATS-6 and ATS-5 were in geosynchronous orbit with a separation of about 11 deg longitude. A ground-based campaign was undertaken by Eather, Mende, and Judge to observe aurora at the foot of the ATS field line with a variety of instruments. Initial results support the injection boundary hypothesis of Mauk and McIlwain. Also during this period, the Cs-ion thrusters on both spacecraft were exercized in a deliberate attempt to control the spacecraft potential. Some success was achieved, and the presence of a sheath with a potential minimum about ATS-5 has been demonstrated (the sheath about ATS-6 is observed directly).

Deforest, S. E.↗
NASA NTRS1977

Theoretical aspects of sounder antenna operation

The fundamental problem of calculating the radiation from an electric dipole immersed in a magnetoplasma is one of solving Maxwell's equations coupled to the self-consistent field-particle equations describing the plasma. For very small applied antenna voltages, the plasma dynamics may be linearized, and the plasma reaction currents computed self-consistently in terms of the voltage on the antenna. Thus, the short dipole can be idealized as a point dipole of moment P sub o = C sub a, V sub o el, where C sub a is the antenna element-to-plasma capacitance for a sheath of dimensions roughly several plasma Debye lengths and el is the dipole element's length. In actual practice, it is at this point that the first difficulty with the antenna theory arises. Even for small signals, the sheath around such an antenna will exhibit a complex impedance. For larger signals, nonlinear effects appear. Attemps are made to solve this problem.

Fredricks, R. W.↗
NASA NTRS1977

Induced emission of Jupiter's decametric radiation by Io-accelerated electrons

A source mechanism for the Io-modulated component of the Jovian decametric radiation is proposed on the basis of the model where electrons in the Io flux tube (IFT) can be accelerated by Io's sheath. It is suggested that a significant fraction of the Io-sheath-accelerated electrons can have pitch angles greater than the atmospheric loss cone and therefore become trapped in the IFT. These electrons have flat helical orbits near their mirror points and can give rise to induced emission of extraordinary-mode radiation with frequencies close to the local electron gyrofrequency. The excitation mechanism is primarily due to the interaction of electromagnetic waves with the electrons via a relativistic gyroresonance which arises because of the momentum dependence of the gyrofrequency. Emphasis is put on the case of nearly perpendicular propagation because it is consistent with the observed source regions and beaming pattern of the decametric emissions. The energy requirement is satisfied within the context of the theory. It is also shown that emission occurs in regions close to the planet above the ionosphere.

Wu, C. S.↗