Engineering Papers⌕ Search

SEARCH · Engineering Papers

Results for “bidirectional”

Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 433 records · Page 24

JPSS-1 VIIRS Solar Diffuser Witness Sample BRF Calibration Using a Table-Top Goniometer at NASA GSFC

In support of the prelaunch calibration of the Joint Polar Satellite System-1 (JPSS-1) Visible Infrared Imaging Radiometer Suite (VIIRS), the Bidirectional Reflectance Factor (BRF) and Bidirectional Reflectance Distribution Function (BRDF) of a VIIRS solar diffuser (SD) witness sample were determined using the table-top goniometer (TTG) located in the NASA GSFC Diffuser Calibration Laboratory (DCL). The BRF of the sample was measured for VIIRS bands in the reflected solar wavelength region from 410 nm to 2250 nm. The new TTG was developed to extend the laboratory’s BRF and BRDF measurement capability to wavelengths from 1600 to 2250 nm and specifically for the VIIRS M11 band centered at 2250 nm. We show the new features and capabilities of the new scatterometer and present the BRF and BRDF results for the incident/scatter test configuration of 0°/45° and for a set of angles representing of the VIIRS on-orbit solar diffuser calibration. The BRF and BRDF results of the SD witness were used to assist in finalizing the set of BRF values of J1 VIIRS SD to be used on-orbit. Comparison of the BRF results between the JPSS-1 VIIRS SD witness sample and the flight SD panel was made by varying different sample clocking orientations and by analyzing the ratio of BRF to total hemispherical reflectance in effort to minimize the uncertainty of the extrapolated flight BRF value at 2250 nm. Furthermore, differences between the prelaunch BRF results and those used in the VIIRS on-orbit BRF lookup table were examined to improve the VIIRS BRF calibration for future missions.

Zeng, Jinan↗

The Harmonized Landsat and Sentinel-2 Surface Reflectance Data Set

The Harmonized Landsat and Sentinel-2 (HLS) project is a NASA initiative aiming to produce a VirtualConstellation (VC) of surface reflectance (SR) data acquired by the Operational Land Imager (OLI) and MultiSpectral Instrument (MSI) aboard Landsat 8 and Sentinel-2 remote sensing satellites, respectively. The HLS products are based on a set of algorithms to obtain seamless products from both sensors (OLI and MSI): atmospheric correction, cloud and cloud-shadow masking, spatial co-registration and common gridding, bidirectional reflectance distribution function normalization and spectral bandpass adjustment. Three products are derivedfrom the HLS processing chain: (i) S10: full resolution MSI SR at 10 m, 20 m and 60 m spatial resolutions; (ii)S30: a 30 m MSI Nadir BRDF (Bidirectional Reflectance Distribution Function)-Adjusted Reflectance (NBAR);(iii) L30: a 30 m OLI NBAR. All three products are processed for every Level-1 input products from Landsat 8/OLI (L1T) and Sentinel-2/MSI (L1C). As of version 1.3, the HLS data set covers 10.35 million km2 and spans from first Landsat 8 data (2013); Sentinel-2 data spans from October 2015. The L30 and S30 show a good consistency with coarse spatial resolution products, in particular MODIS Collection 6 MCD09CMG products (overall deviations do not exceed 11%) that are used as a reference for quality assurance. The spatial co-registration of the HLS is improved compared to original Landsat 8 L1T and Sentinel 2A L1C products, for which misregistration issues between multi-temporal data are known. In particular, the resulting computed circular errors at 90% for the HLS product are 6.2 m and 18.8 m, for S10 and L30 products, respectively. The main known issue of the current data set remains the Sentinel-2 cloud mask with many cloud detection omissions. The cross-comparison with MODIS was used to flag products with most evident non-detected clouds. A time series outlier filtering approach is suggested to detect remaining clouds. Finally, several time series are presented to highlight the high potential of the HLS data set for crop monitoring.

Landsat Sentinel-2↗

Coupled Retrieval of Aerosol Properties and Surface Reflection Using the Airborne Multi-Angle SpectroPolarimetric Imager (AirMSPI)

The Airborne Multi-angle SpectroPolarimetric Imager (AirMSPI) has been flying aboard the NASA ER-2 high altitude aircraft since October 2010. In step-and-stare operation mode, AirMSPI acquires radiance and polarization data at 355, 380, 445, 470*, 555, 660*, 865*, and 935 nm (* denotes polarimetric bands). The imaged area covers about 10 km by 10 km and is observed from 9 view angles between ±67° off of nadir. We have developed an efficient and flexible code that uses the information content of AirMSPI data for a coupled retrieval of aerosol properties and surface reflection. The retrieval was built based on the multi-pixel optimization concept, with the use of a hybrid radiative transfer model that combines the Markov Chain and adding/doubling methods. The convergence and robustness of our algorithm is ensured by applying constraints on (a) the spectral variation of the Bidirectional Polarization Distribution Function (BPDF) and angular shape of the Bidirectional Reflectance Distribution Function (BRDF); (b) the spectral variation of aerosol optical properties; and (c) the spatial variation of aerosol parameters across neighboring image pixels. Our retrieval approach has been tested using over 20 AirMSPI datasets having low to moderately high aerosol loadings (~0.02<AOD550-nm<~0.45) and acquired during several field campaigns. Results are compared with AERONET aerosol reference data. We also explore the benefits of AirMSPI’s ultraviolet and polarimetric bands as well as the use of multiple view angles.

Xu, Feng↗

Tools for Performing SBG hyperspectral Observing System Simulation Experiment

One of NASA’s Decadal Survey mission, Surface Biology and Geology (SBG), will include a hyperspectral remote sensing imager, which has a very high spatial resolution and a wide spectral coverage (from UV to Near IR). Unprecedented large data volumes will be generated by the SBG hyperspectral instrument. Before the launch of the new satellite, an Observing System Simulation Experiment (OSSE) can be used to study different designs of the new satellite system. One of the key components in an OSSE study is a radiative transfer model (RTM) or forward model. In this presentation, we will describe a Principal Component-based Radiative Transfer Model (PCRTM) which is capable of simulating atmospheric (TOA) radiance or reflectance spectra from far IR to visible and UV spectral regions (50 wavenumber to 30000 wavenumber) quickly and accurately. Multiple scattering from multiple layers of clouds/aerosols are included in the model. The PCRTM has a very good accuracy relative to reference line-by-line radiative transfer models (LBLRTM), and it saves 3-4 orders of magnitude computational time relative to LBLRTM or MODTRAN. The PCRTM model has been successfully used to analyze large volumes of data from hyperspectral sensors such as AIRS, CrIS, and IASI. It has also been used to perform OSSE studies for the Climate Absolute Radiance and Refractivity Observatory (CLARREO) mission. Another useful tool for the OSSE is surface Bidirectional Reflectance Distribution Function (BRDF) database. It is very crucial for the SBG OSSE to include realistic BRDF spectra. Currently, most of the surface reflectance spectra such as those in the ECOSIS and ECOSTRESS are measured at specific observation geometries. We have developed a hyperspectral bidirectional reflectance (HSBR) model which combines Ross-Li BRDF model with the existing reflectance spectral libraries using a principal component analysis. This HSBR model can provide realistic BRDF spectra under various observation conditions. It can also be used to generate realistic BRDF spectra using measurements from multi-band imagers or spectrometers such as MODIS or VIIRS.

Xu Liu↗

Compact Electric Bi-Directional Pulley Actuator

While significant work has been dedicated to the study of potential uses of in situ resources once mined, the methods to mine the raw materials has not been fully explored. This paper presents the first attempt at producing a drop in replacement for hydraulic cylinders on heavy construction equipment with an electromechanical actuator utilizing pulleys for the conversion of a single motor’s rotational motion to bidirectional linear motion. By utilizing a twin block and tackle arrangement and paired spools, the system can generate bidirectional motion nearly identical to a hydraulic cylinder. More importantly, the use of rope builds in shock resistance to the system which is often a weak point for screw style actuators. Significant work remains to refine the concept into flight hardware, but the concept shows promise. The produced actuator can be applied or scaled for near direct replacement of hydraulic cylinders on terrestrial mining and construction equipment for faster development of off Earth use.

Kevin Peterson↗

Polarimetric sensitivity of light-absorbing carbonaceous aerosols over ocean: A theoretical assessment

Visible-light-absorbing carbonaceous aerosols within the boundary layer affect the radiance and polarization states of the radiation at the top of the atmosphere. Remote sensing from suborbital and satellite-based platforms utilizes these radiance and polarization signals to retrieve the key properties of these aerosols. Recent retrieval algorithms have shown a progressive trend toward including multi-angular and multi-spectral polarimetric measurements to produce better retrieval accuracy in comparison to those using measurements based on a single viewing angle. Here, we perform a theoretical investigation of the top of atmosphere (TOA) radiance-related reflectance factor (bidirectional reflectance factor (BRF)) and the two types of polarimetry-related factors (polarized bidirectional reflectance factor (pBRF) and the degree of linear polarization (DoLP)) for different types of atmospheric light-absorbing carbonaceous aerosols as a function of particle size distribution. We selected three polarimetric bands corresponding to those utilized by NASA's Airborne Multiangle SpectroPolarimetric Imager (AirMSPI)—near-UV (470 nm), visible (660 nm), and near-infrared (865 nm)—for our simulations which were performed over ocean surface using the successive order of scattering (SOS) algorithm coupled to a Lorenz-Mie aerosol optics model. The analysis of particle phase matrix elements indicates a close relationship between the angular dependencies of DoLP and associated phase matrix components at the shortest polarimetric band (470 nm). Using Jacobian analysis, we find that the radiance- and polarimetry-related reflectance factors of weakly light-absorbing aerosols, such as brown carbon, are more sensitive to changes in particle size and imaginary refractive index in comparison with those of black carbon, which is strongly light-absorbing. Our results suggest that the DoLP data could be used by future retrieval algorithms for reliably estimating microphysical properties of absorbing carbonaceous aerosols with imaginary refractive index less than 0.4.

refractive index↗

An Ensemble Investigation of the Causes for Regional Air-Quality Model Critical Load Exceedances Prediction Variability in European and North American Domains Using Diagnostics From Phase 4 of the Air Quality Model Evaluation International Initiative

We summarize tentative findings from multi air quality model ensembles for the years 2009 and 2010 in Europe (EU), and 2010 and 2016 in North America (NA), under AQMEII-4. The model predictions of sulphur and nitrogen deposition were used to estimate exceedances of critical loads for acidification and eutrophication, to show the extent to which the ensemble members agree in the magnitude and the trend of ecologically meaningful impacts. Model exceedance variability was analyzed using AQMEII-4 diagnostics. Evaluation against concentration and wet deposition observations, coupled with these diagnostics, identified specific process representations as the causes for variability between model predictions and for reduced model performance. All models predicted reductions in ecosystem acidification impacts in North America between the years 2010 and 2016, in accord with SO2 emissions reduction legislation which started in 2010 (SO2 SIP) However, all models in EU and NA domains had net negative biases for wet deposition of sulphur and nitrogen relative to observations. The wet S deposition average mean bias for the NA ensemble was -0.17 eq ha-1 d-1, and for the EU ensemble -1.15 eq ha-1 d-1. The NA daily wet deposition average mean bias for NH4+ was -0.37 eq ha-1d-1; EU -1.19 eq ha-1 d-1. The daily NA wet NO3- deposition average mean bias was -0.24 eq ha-1d-1; EU -0.69 eq ha-1 d-1. The members of the ensemble diverged (factor of 10) in their North American predictions for Ndep and consequently their eutrophication exceedances. The models with the highest eutrophication predictions also predicted the highest levels of gas-phase ammonia dry deposition (standard deviation of ammonia dry deposition flux across ensemble members was larger than the ensemble average). These models also had negative biases of predicted ammonia concentrations; average mean biases of -0.63 (satellite NH3) and -0.85 ppbv (surface NH3) compared to ensemble averages of -0.30 and -0.34 ppbv. Diagnostics showed that these differences resulted from the manner in which bidirectional ammonia fluxes were parameterized within these models. The second largest source of NA eutrophication prediction variability were models with positive biases in particulate ammonium and nitrate concentrations, and higher particle nitrogen deposition levels ( particle ammonium concentration bias +0.35 ug m-3; ensemble bias +0.15 ug m-3). We believe two factors may have led to these latter overestimates: higher levels of fine mode particle nitrate formation compared to other models (due to the use of an inorganic heterogeneous chemistry algorithm which did not take base cation chemistry into account), and updates to particle dry deposition velocities carried out in the absence of concurrent updates to wet scavenging algorithms. The relative importance of dry gas, dry particulate, and wet deposition towards total sulphur and nitrogen deposition totals differed between EU and North American domains, though all models had negative biases in wet deposition as noted above. Parallel and subsequent work suggests that multiphase hydrometeor scavenging may improve model wet deposition performance. An increased research focus is recommended for four model processes: multiphase hydrometeor scavenging, ammonia bidirectional fluxes, base cation chemistry and emissions, and particle dry deposition.

regional air-quality model↗

High-Frequency Signature-Based Fault Detection for Future MV Distribution Grids: Preprint

Increased deployment of Distributed Energy Resources (DERs) and microgrids are significantly changing the operation of distribution grid. Increasing penetration of power electronics based DERs also raise several operational problems such as protection coordination issues, voltage raise, recloser synchronization, stability, and frequency control. Legacy systems are designed for unidirectional power flow. Bidirectional power flows, and varying short circuit levels are some of the major challenges in implementing these legacy protection schemes for future power grid with high penetration of power electronics based DERs. Inverter based DER’s can contribute to a maximum of 2 p.u. fault current. Interconnection standards might require even lower fault current contribution. If these protection related challenges are not addressed, it will result in limiting the penetration of DERs in distribution feeders. In this paper, the feasibility of high frequency signature generated by transients is evaluated to identify and locate faults in a distribution system. The paper will discuss the need for an accurate line modeling approach to accurately capture the high frequency signatures. These high frequency signatures are naturally occurring electromagnetic transients for events such as switching events in the network, faults events, etc. This is different than that implemented in transmission systems as the proposed approach utilizes advanced signal processing techniques to retrieve valuable information from the high frequency signatures generated during faults and does not just rely on time delay measurements between reflections of the high frequency signals. In the full paper, information of a modified IEEE distribution feeder test system to accurately model the high frequency signatures will be provided. The distribution lines will be modeled using universal line modeling (ULM) technique in an electromagnetic transients program (EMTP) modeling platform. Different test cases will be simulated and these test cases will include different fault types, multiple fault locations, capacitor bank switching, etc. The feeder system modeled for these simulations will also include power electronics based DERs. Additionally, experimental results demonstrating the high frequency signatures generated due to a transient event in a medium voltage (13.8 kV) distribution line will also be presented in the full paper. Furthermore, the analysis combined with simulation and experimental results in the full paper will help demonstrate the feasibility and efficacy of the proposed protection scheme. The developed protection scheme will not just help solve problems generated by bidirectional current flow, varying short-circuit current capacity, but also help in proliferation of DERs in distribution systems.

41 EE - Solar Energy Technologies Office (EE-4S)↗

Field validation of isotropic analytical models for simulating fabric shades

Fabric roller shades are common shading materials used in commercial and residential buildings. Accurately characterizing and modeling shades helps practitioners select the appropriate product and its control strategy based on climate and occupants' priorities, such as visual comfort and view to outdoors. Previous studies established a generalized method for modeling complex fenestration systems using data-driven tabulated bidirectional scattering distribution functions. However, deploying such a method at scale to all fabric shading products on the market is too costly and time-consuming. Analytical models that are based on a limited set of measurements (e.g., normal-normal and normal-hemispherical visible transmittance and reflectance, and directional cut-off angles) can be used to model the wide variety of shading products on the market. This study evaluates the performance of two isotropic analytical models, Roos-Wienold and Modified-Kotey, for modeling fabric roller shades, with a focus on the model's ability to predict occupant visual comfort. The performance evaluation was conducted through laboratory and field measurements and simulations. The results showed that both models are sufficient for predicting vertical illuminance at seated eye-level. Roos-Wienold model was able to predict binary visual comfort classification (glare/no-glare) under a wide range of luminance conditions, while Modified-Kotey model did not perform as well under high-contrast low-adaptation conditions. Both models are insufficient in predicting visual comfort at a four-point scale (e.g., imperceptible, perceptible, disturbing, intolerable). The two isotropic models become less accurate when the fabric exhibits high anisotropy.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

Field validation of data-driven BSDF and peak extraction models for light-scattering fabric shades

Shading and daylighting systems affect cooling, heating, and lighting energy use by modulating solar radiation through the building façade. Characterizing shading systems holistically and accurately helps designers and engineers evaluate shading systems to achieve energy and non-energy performance goals. These complex fenestration systems can be modeled using Bidirectional Scattering Distribution Functions (BSDF), which map incident radiation to hemispherical distributions of outgoing radiation. Data-driven, tabulated BSDFs are derived from interpolated goniophotometer measured data, then sampled during the raytracing calculation. A peak extraction (PE) algorithm was developed to circumvent limits in BSDF angular resolution, where the specular peak is extracted during simulation by evaluating the BSDF in the through direction and surrounding region. The objective of this study was to validate this measurement and modeling workflow using field monitored data from a full scale testbed with eleven installed fabrics of different weaves, openness factors, and colors and assess the accuracy of the workflow under different adaptation and contrast conditions. Test conditions were limited to clear sky conditions with the sun in the field of view. Results showed that, for tensor tree datasets, vertical illuminance, solar luminance (2.5° apex), and daylight glare probability (DGP) were predicted to within a mean bias error (MBE) error of -456 lx (-12.3%), -3.46e5 (-38.4%), and -0.042 (-7.8%) when full PE occurred. With a binary classification of glare/ no glare, DGP was predicted accurately with a true positive rate of 0.98 and true negative rate of 1.0 using tensor tree data and less accurately with Klems BSDF data, particularly for cases of no glare. The workflow may be of insufficient accuracy to distinguish borderline performance between fabrics using the four-point glare scale, particularly under low adaptation, high contrast daylit conditions. Errors were due to reductions in peak shape and intensity across the BSDF interpolation and data reduction workflow. Future work is needed to better preserve measurement fidelity during interpolation and sampling, which in turn will improve PE performance.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

Advocating for view and daylight in buildings: Next steps

With the exponential growth in population and commensurate increased density in urban cities, access to daylight and views to nature is being severely curtailed in buildings. In parallel, increasingly urgent demands to sharply reduce building energy use and associated greenhouse gas emissions are being made to mitigate climate change. There are many challenges and performance tradeoffs associated with the building facade (i.e., daylight and view versus solar and glare control); increased prioritization of health and well-being as a fundamental human requirement could adversely affect building energy-efficiency. Given the current state of knowledge on the effects of daylight and view on health and well-being in buildings, we identify critical needs in research, tools and technologies that if satisfied may enable more effective use of daylight and view in buildings within the constraints of climate change. Lack of knowledge regarding the complex causal mechanisms of window views on human factors is a severely limiting factor in forward progress. Current models and methods to derive bidirectional scattering distribution functions (BSDFs) will need to be modified. Developers of energy-efficient window technologies will need more guidance to shape product development. Advanced window technologies and integrated design can enable attainment of both health and well-being and net zero energy goals, but considerable work will be needed to make such options turnkey and broadly available.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

Light scattering by V 4 O 7 film across the metal–insulator transition

The experimental study of the angle-resolved hemispherical light scattering by V 4 O 7 film within a broad temperature range across metal–insulator transition reveals complex structural reorganization of the film deposited on the c-cut sapphire crystal. The bidirectional scattering distribution function and the surface autocorrelation function were obtained from scattering data to visualize statistics of the spatially resolved contributions of optical inhomogeneities in normal and lateral directions to the surface. The measurements reveal an anisotropic surface roughness distribution due to the twinned domain structure, with significant anisotropy changes across the phase transition. The V 4 O 7 film deposited on sapphire leads to a polydomain structure, minimizing elastic strain energy with distinct multiscale distributions of surface domains. Near T c , the material shows the lowest roughness but the highest lateral disorder of the surface.

36 MATERIALS SCIENCE↗

Design, Optimization, and Validation of GaN-Based DAB Converter for Active Cell Balancing in BTMS Applications

This paper focuses on the design of a bidirectional dual active bridge (DAB) DC/DC converter that utilizes Gallium Nitride (GaN) switches as active components. In the existing literature, MOSFET-based DAB for active cell balancing is available, but GaN-based DAB converter for active cell balancing is still new. The proposed modular isolated GaN-based DAB converter is designed as an individual module of active cell balancing for behind-the-meter storage (BTMS) applications, targeting high-power charging stations. Modular isolated converters are connected to each cell (low voltage bus), and each cell is connected in series to build up a battery module. According to the reference current command of supervisory control, each DAB converter can transfer power back and forth through the high voltage (HV) bus to balance the State of Charge (SoC) between the cells. Each module DAB converter is designed at a 50 W power rating. Switch power and transformer losses are analyzed for different switching frequencies, showing the optimum switching frequency for minimum losses. Furthermore, the procedure to select the required gate driver and the PCB layout optimization are discussed. Finally, the DAB performance analysis of GaN-based DAB and Si-based DAB is provided for a battery module operating with a LiFeMnPO4 prismatic cell with 3.2V 20Ah rated values.

active cell balancing↗

Optimized Highly Efficient SSCB Using Organic Substrate Packaging for Electric Vehicle Applications

Solid State Circuit Breakers (SSCBs) are an attractive protection solution for their arcless current interruption and fast actuation speeds over mechanical breakers. This paper proposes a Bidirectional SSCB (BSSCB) with a thermally defined and digitally controlled current time profile for fault protection in EV and other low-voltage DC systems. The paper proposes an organic packaging approach utilizing flex circuitry to develop a reliable, cost-effective power module for BSSCBs. The paper studies transient heat transfer in the power modules using finite element analysis (FEA). An RC themal ladder network is extracted to define a fusing curve. To demonstrate and verify the design, a 1kV/50 A SiC MOSFET BSSCB prototype is fabricated and tested, having a power density of 60 W/cm3 and 4x reduction in form factor over presently researched breakers. Also, given are results for 750 V/150 A operation showing interruption in 2.4 μs.

organic power packaging↗

Analysis and Control of a Three-Port Converter with High-Frequency AC Link for Hybrid Unmanned/Autonomous Aerial Vehicles

In this study, a three-port resonant converter with a high-frequency isolation link for hybrid unmanned/autonomous aerial vehicle (UAV/AAV) applications is analyzed. The rectified engine-generator set output supplies the first input ports while the other is connected to a battery energy storage system. Input ports consist of half-bridge high-frequency inverters, whereas output port utilizes a semi-bridgeless active rectifier. This rectifier is formed by replacing rectifier low-side diodes with active switching devices. A phase-shift duty-cycle control is implemented for switching signals to regulate the power share and flow between ports. The proposed control system implemented achieves zero voltage switching (ZVS), reduces switching losses, and minimum current flow optimization ensures regulation in a wide range of output power. Compared to the existing bidirectional pulse width modulation (PWM) converters, the proposed system decreases the number of power conversion stages and provides centralized control without needing communications between the ports. The proposed control technique operating in closed-loop control achieves the power flow between ports under various load conditions. Finally, theoretical analysis demonstrates the proposed converter’s viability and laboratory experimental validations at 500 W full power.

33 ADVANCED PROPULSION SYSTEMS↗

Data-Driven Affinely Adjustable Robust Volt/VAr Control

Recent years have seen the increasing proliferation of distributed energy resources with intermittent power outputs, posing new challenges to the voltage management in distribution networks. To this end, this paper proposes a data-driven affinely adjustable robust Volt/VAr control (AARVVC) scheme, which modulates the smart inverter’s reactive power in an affine function of its active power, based on the voltage sensitivities with respect to real/reactive power injections. To achieve a fast and accurate estimation of voltage sensitivities, we propose a data-driven method based on deep neural network (DNN), together with a rule-based bus-selection process using the bidirectional search method. Our method only uses the operating statuses of selected buses as inputs to DNN, thus significantly improving the training efficiency and reducing information redundancy. Finally, a distributed consensus-based solution, based on the alternating direction method of multipliers (ADMM), for the AARVVC is applied to decide the inverter’s reactive power adjustment rule with respect to its active power. Only limited information exchange is required between each local agent and the central agent to obtain the slope of the reactive power adjustment rule, and there is no need for the central agent to solve any (sub)optimization problems. Finally, numerical results on the modified IEEE-123 bus system validate the effectiveness and superiority of the proposed data-driven AARVVC method.

24 POWER TRANSMISSION AND DISTRIBUTION↗

Transcriptomic and metabolomic changes in postharvest sugarbeet roots reveal widespread metabolic changes in storage and identify genes potentially responsible for respiratory sucrose loss

Endogenous metabolism is primarily responsible for losses in sucrose content and processing quality in postharvest sugarbeet roots. The genes responsible for this metabolism and the transcriptional changes that regulate it, however, are largely unknown. To identify genes and metabolic pathways that participate in postharvest sugarbeet root metabolism and the transcriptional changes that contribute to their regulation, transcriptomic and metabolomic profiles were generated for sugarbeet roots at harvest and after 12, 40 and 120 d storage at 5 and 12°C and gene expression and metabolite concentration changes related to storage duration or temperature were identified. During storage, 8656 genes, or 34% of all expressed genes, and 225 metabolites, equivalent to 59% of detected metabolites, were altered in expression or concentration, indicating extensive transcriptional and metabolic changes in stored roots. These genes and metabolites contributed to a wide range of cellular and molecular functions, with carbohydrate metabolism being the function to which the greatest number of genes and metabolites classified. Because respiration has a central role in postharvest metabolism and is largely responsible for sucrose loss in sugarbeet roots, genes and metabolites involved in and correlated to respiration were identified. Seventy-five genes participating in respiration were differentially expressed during storage, including two bidirectional sugar transporter SWEET17 genes that highly correlated with respiration rate. Weighted gene co-expression network analysis identified 1896 additional genes that positively correlated with respiration rate and predicted a pyruvate kinase gene to be a central regulator or biomarker for respiration rate. Overall, these results reveal the extensive and diverse physiological and metabolic changes that occur in stored sugarbeet roots and identify genes with potential roles as regulators or biomarkers for respiratory sucrose loss.

59 BASIC BIOLOGICAL SCIENCES↗